Science topic

Columbidae - Science topic

Columbidae is a family in the order COLUMBIFORMES, comprised of pigeons or doves. They are BIRDS with short legs, stout bodies, small heads, and slender bills. Some sources call the smaller species doves and the larger pigeons, but the names are interchangeable.
Questions related to Columbidae
  • asked a question related to Columbidae
Question
4 answers
Hello, everyone
I've recentely isolated bacteria from pigeon pea (Cajanus cajan) nodules on YMA medium and then transferred some colonies to YMB (Yeast Mannitol Broth) medium. This is the first time I'm using this medium, and I'm a bit unsure about working with it. After 48 hours of inoculation in liquid medium, I noticed that most of the tubes were slightly turbid but not very much, especially when compared to other more "yellowish" media. They still seem quite translucent. I would like to know if this is normal and whether my bacteria have actually grown in the medium.
Thank you in advance for your assistance.
Best regards, Daniel
Relevant answer
Answer
Yeast Mannitol Broth (YMB) is a commonly used medium for cultivating rhizobia, the bacteria associated with legume nodules, including those of pigeon pea (Cajanus cajan). Understanding the appearance of YMB after bacterial inoculation and growth can help you determine whether your bacteria have successfully grown in the medium. Here is a comprehensive and detailed explanation of what to expect and how to assess bacterial growth in YMB:
Appearance of YMB Medium After Bacterial Inoculation
1. Initial Observation:
  • Fresh YMB: Before inoculation, YMB is typically clear and light amber in color. It is a nutrient-rich medium designed to support the growth of rhizobia and other similar bacteria.
  • After Inoculation: Upon inoculating the YMB with bacterial colonies, you might initially see no change in the medium's appearance. This is normal as the bacteria need time to acclimate and begin dividing.
2. Turbidity Development:
  • Slight Turbidity: After 48 hours of incubation, slight turbidity is a positive sign indicating bacterial growth. Rhizobial cultures in YMB can sometimes appear only slightly turbid, especially if the initial inoculum was not very dense.
  • Comparison with Other Media: YMB may not become as turbid as other media like LB (Luria-Bertani) broth, which can support rapid and dense bacterial growth. YMB's composition supports rhizobial growth at a rate that may result in moderate turbidity.
3. Factors Affecting Turbidity:
  • Inoculum Size: The initial number of bacteria transferred to the YMB medium affects the rate and extent of turbidity. A smaller inoculum will result in slower growth and less turbidity.
  • Incubation Conditions: Temperature, shaking speed (if using a shaker), and the initial health of the bacterial colonies all influence the growth rate. Optimal conditions for rhizobial growth are typically around 28-30°C with moderate shaking to ensure aeration.
  • Growth Phase: Bacteria in the exponential phase will produce more noticeable turbidity compared to those in the lag phase. It might take a few days for visible turbidity to develop fully.
Confirming Bacterial Growth in YMB
1. Visual Inspection:
  • Translucency: Slight turbidity and a translucent appearance are normal. Compare inoculated tubes with an uninoculated control tube of YMB to detect subtle differences in turbidity.
  • Sediment Formation: After a longer incubation period, you might observe a slight sediment at the bottom of the tube, which is another indication of bacterial growth.
2. Microscopic Examination:
  • Smear Preparation: Take a small sample from the YMB tube and prepare a smear on a microscope slide. Stain it with a simple stain (e.g., crystal violet) to visualize the bacterial cells under a microscope.
  • Cell Morphology: Confirm the presence of bacteria by checking for the characteristic morphology of rhizobia (e.g., rod-shaped, sometimes with slight pleomorphism).
3. Viable Count:
  • Plating on Solid Medium: To quantify bacterial growth, you can perform a serial dilution of the YMB culture and plate it on YMA (Yeast Mannitol Agar) or another suitable solid medium.
  • Colony Counting: After incubation, count the colonies to estimate the number of viable bacteria present in the YMB culture.
4. Growth Curve Analysis:
  • Spectrophotometry: Measure the optical density (OD) at 600 nm (OD600) using a spectrophotometer at regular intervals. Plotting OD600 over time will give you a growth curve, indicating different growth phases (lag, log, stationary).
  • Interpreting OD Values: An increase in OD600 over time indicates bacterial growth. Typical OD600 values for visible turbidity in rhizobial cultures might range from 0.1 to 1.0, depending on the growth conditions.
Troubleshooting Growth Issues
1. Medium Quality:
  • Check Medium Composition: Ensure that the YMB medium was prepared correctly and that all components were added in the right proportions. Autoclaving should be done properly to sterilize the medium without degrading nutrients.
  • pH Levels: Verify the pH of the medium, as rhizobia prefer slightly acidic to neutral pH (around 6.8-7.0).
2. Inoculum Quality:
  • Viability of Initial Culture: Confirm that the bacterial colonies transferred from YMA to YMB were viable and actively growing. Old or stressed colonies may take longer to adapt and grow in a new medium.
3. Incubation Conditions:
  • Temperature and Aeration: Ensure that the incubation temperature is optimal for rhizobial growth (typically 28-30°C) and that the cultures are adequately aerated if using a shaker.
Summary
  • Slight Turbidity: It is normal for YMB to show slight turbidity after 48 hours, indicating bacterial growth.
  • Comparison: Compare with an uninoculated control to assess turbidity.
  • Microscopic Confirmation: Check for bacterial cells under a microscope.
  • Viable Count: Plate dilutions on solid medium to confirm growth.
  • Growth Curve: Measure OD600 to monitor growth over time.
By following these guidelines, you can confidently assess whether your bacteria have grown in YMB medium and ensure that your experimental conditions are optimal for their cultivation.
  • asked a question related to Columbidae
Question
4 answers
Dear Researchers,
I am reaching out to share my latest work called Recursive Data Compression (2nd draft). I am fully aware of the skepticism that accompanies groundbreaking claims, especially those that propose solutions to problems deemed insurmountable or impossible to answer. Yet, it is in the spirit of scientific exploration and the relentless pursuit of knowledge that I invite you to engage with my research, even though I foresee some ridicule.
This work is not just an academic exercise but a genuine attempt to expand the boundaries of our understanding of data compression. I encourage you to approach this paper with an open mind, to delve beyond the surface, and to contribute your valuable insights and critiques. (Note: Please don't mention Pigeon Holes or Kolmogorov unless you have read the paper).
Your expertise however is crucial to the refinement and progression of my research. I am seeking not just validation but constructive feedback, challenging questions, and rigorous debate that can only come from the sharing of ideas.
Please give this paper a chance, look past any preconceptions, and join me in a dialogue that could potentially reshape our approach to data compression.
I am looking forward to your thoughts and feedback.
Warm regards,
Archie
Relevant answer
Answer
Nope. It will (can) always be the exact same data array. You seem to get it.
  • asked a question related to Columbidae
Question
4 answers
This discussion focuses on two questions:
1. How to technically teach the process of writing code and what are the implications of generative AI on this process?
2. Would you begin teaching data analysis with GUI tools (like excel or google spreadsheets) or dove straight into programming?
I am very interested in hearing your opinion
Relevant answer
Answer
I would start right away to teach data analysis using GNU R, without spreadsheets. There are good learning materials, as https://r4ds.had.co.nz/ (Tidyverse approach) or https://github.com/matloff/fasteR (base R). Generative AI is not relevant here in my opinion, first you need to understand data summarization, filtering, grouping, ploting, all the basics. Then statisitical inference. Maybe in your next courses it would be good for students to teach them SPSS / SAS / whatever is used in the industry. Spreadsheets are great for accounting tasks.
  • asked a question related to Columbidae
Question
5 answers
The spacing among different accessions of pigeon pea to control cross-pollination among those accessions.
Relevant answer
Answer
Ok, probably wind and insects are the major agents.
  • asked a question related to Columbidae
Question
4 answers
Good day everyone, I would just like to ask if anyone has any protocols or information related to Lugols staining, in relation to pigeon pea seeds? I have found some protocols related to staining the leaves of various plants. The difficulty arises when looking for a protocol specifically for seeds.
  • asked a question related to Columbidae
Question
5 answers
I would possibly like help in extracting RNA from pigeon pea leaves. I am currently using the Maxwell 16 Lev plant RNA extraction kit. I have conducted 2 extractions yet both yielding low concentration (+/- 50 ng/ul) and purity (1.4- 1.6). I also treated my cDNA with thermofishers DNase treatment kit yet although the purity improves, I am still unable to visualise my samples on a 1% gel. I plan on synthesizing cDNA from this RNA and cloning into pGEMT Easy afterwards.
I am all ears for any possible troubleshooting.
Relevant answer
Answer
Thank you everyone for the replies, I have managed to extract high-quality RNA (720ng/ul A260/280 ratio of 2,07) by following the same protocol but centrifuging my samples after they have been processed by the RNA robot. This allows for any metal particles to form a pellet and thus I can aliquot my supernatant into another tube. This seems to work well for me. Once again thank you all for your help!
  • asked a question related to Columbidae
Question
4 answers
The foraging behavior of European honey bee on Pigeon pea......?
Relevant answer
Answer
  • asked a question related to Columbidae
Question
6 answers
Hey everyone, I just need some help with finding homologs for an organisms I am research. These would be the ADP Glucose Pyrophosporylase gene and the Glucan Water Dekinase genes, I have obtained the Arabidopsis sequences for the genes and now I want to use the Homolog function on Legume IP V3 to find the related genes in Pigeon peas (Cajanus cajan).
I have been trying to change my parameters but nothing seems to help.
Relevant answer
Answer
  • asked a question related to Columbidae
Question
11 answers
We're starting to use touchscreens with pigeons in an arrangement I have not used before, so I need your expertise. The arrangement is a large touchscreen at the other end of the experimental chamber from the magazine. The Python autoshaping program that I have written does the usual things -- presents a "key" of randomized colors and locations on the black screen, followed by food delivery. The chambers we use are lit with normal room lighting
Will this work? It worries me that magazine training will engender hanging around by the hopper, so detecting the key onset may be difficult.
If any of you have expertise in autoshaping under such conditions, I'd appreciate any information
Thanks - michael
Relevant answer
  • asked a question related to Columbidae
Question
3 answers
Trying to record tutorial videos from lightboard. Presently video is to be flipped 1800 using video editing software. trying to use optics to flip the video so that live classes can be conducted.
  • asked a question related to Columbidae
Question
1 answer
Effect of intercropping of Sorgum with Pigeon pea on the growth attributes like plant height,number of tillers , dry matter composition, and yield attributes like number of panicles,number of grains per panicle ,weigt of panicle,test weight etc
Relevant answer
Answer
Взаимный посев Сорго с голубиным горохом положительно влияет на обоих культур .
  • asked a question related to Columbidae
Question
3 answers
what effect can be seen on Pigeon pea intercropping system when planted on two different directions like North-east and South-west
Relevant answer
Answer
there will be quantitative changes in the composition of the chemical element
  • asked a question related to Columbidae
Question
8 answers
Which soap is better for skin like dove, lux, lifebuoy etc?
Relevant answer
Answer
Patanjali Aloevera is the best soap.
  • asked a question related to Columbidae
Question
7 answers
One observation is that in any city (those I know) you have humans, of course, but you also have many animals.
Statistics of humans start with population data (for instance demographics) and continue with behavioural data, observed as how humans behave socially or asocially in the smart city environment.
There is however an additional case of life in a smart city: animals. First, animals considered to be "companions" (some of them being "stray", after escaping or being "wild" anyway) in particular gogs and cats. Others like birds flying freely in the sky of the city, could be very different ones: pigeons, sea-gulls, etc. Then you have rats and mice, and insects.
What can be gained from observing these animals about:
-air quality?
[Paris has an ongoing project with bees living on the roof of Tour Montparnasse, one of the tallest skyscrapers of the city. If the population of bees is stable or grows, the air quality is likely to be good. If not, then one has a concern]
-climate/temperature and humidity
-spread of diseases
sampling from stray cats and dogs? foxes (case of Greater London area)
sampling from domestic animals?
Would there be an interest to observe animals rather than humans or vice-versa, for a deeper understanding of the smart city's parameters?
PLEASE CONTRIBUTE your view and experience
Relevant answer
Answer
adding fishes and algs in rivers and ponds of the city
  • asked a question related to Columbidae
Question
3 answers
I was working with the isolation of Alternaria from the pigeon pea. Presently, I got the plates containing the conidia of A. alternata and A. tenuissima in the one culture plate. Please suggest me the ways to separate both these species and get the pure culture of each of these.
Relevant answer
Answer
You can use a spore dilution method to get single-spore colonies, as Alex said above, or you can use the intriguingly weird method that we in the powdery mildew community use to get single spores: by attaching a human eyelash to a glass rod with wax, and, under a dissecting scope, catching a single spore on the tip of the eyelash. Alternaria has chains of spores just like powdery mildews, so it will be possible to catch the tip-most spore in a chain and deposit it on a fresh plate of agar.
  • asked a question related to Columbidae
Question
4 answers
It is known that prey birds have frontal vision (attack) whereas pigeons have lateral vision (defense). Do we have other principles observed among animals, mammals, insects, etc?
Keywords: biology, visual perception, food chain, survival instinct
Relevant answer
Answer
The human vision is particular and I don't think can be compared with other animal race vision. It's a "straight" binocular vision of maximum 220° divided in 4 areas: foveal area, attentive watch area, induced vision area and lateral vision.
As far as you move from the foveal area, the objects perceived will become more grey going slowly to the dark and from distinctive image to totally blurred image.
The attentive watch area, the induced vision area and the lateral vision compose the peripheral vision.
The leading part of the peripheral vision is to detect visual "mass" and to transmit a message to the foveal vision area saying "Hey! Look! There is "something" to see here!".
The leading part of the foveal vision is to catch is "to call" your eyes and your head to turn where the peripheral vision has detected the visual "mass" and to transmit the different "signs" of the perceived image (color, features, shapes, ...) an electric signal through the optic nerve in the to the sensorial memory who will analyze the semiological data and "preselect" the research elements needed by the working memory.
The human eye does not analyze anything, it only transmits the visual information.
Here are 2 links (sorry in French) that explain what I am talking about.
As urban ethologist, I use many times these visual scales in public spaces audits to help architects in terms of use of semiological elements that correspond to the human hierarchy of ocular capture especially in areas like airports, railways stations, commercial malls, etc.
I hope my definition is understandable and not too much scientific.
Wishing you a nice reading.
  • asked a question related to Columbidae
Question
3 answers
Animal self-awareness usually be tested with the classical mirror test ( The mirror test is a measure of self-awareness developed by Gordon Gallup Jr in 1970, and animals which have passed the mirror test are common chimpanzees, bonobos, orangutans, dolphins, elephants, humans and possibly pigeons. ). However, the mirror test may not appropriate for test the self-awareness for invertebrates , especially arthropods, as visual signals usually be not crucial for these creatures, or they may not care about some marked points on their body etc. Is there any possible methods to test the self-awareness of these organisms?
Relevant answer
Answer
One thing you might consider is that, while passing the mirror test is fairly difficult, failing it is easy. You could first expose the animals to a mirror and see if any of them attempt to socialize, mate with, or attack the mirror. This would still assume that arthropods rely on visual cues, but if they do it would serve as a good first step. It would be unfortunate to design a more robust experiment around a mantis shrimp, for example, only to have it attack its reflection right away
  • asked a question related to Columbidae
Question
5 answers
The work published by Oseghale et al (2017), observed that Tropical crops response to photoperiod under natural condition is not the issue of hourly incremental rate (i.e. 8h, 9h, 10h, 11h, 12h or 13h) but a matter of few minutes changes (5 minutes). Consequently, all the previous crop models that used afore mentioned rate will have to be reviewed if they are to be relevant to the natural photoperiod condition in the tropics. The study observed that photoperiod modifies the temperature response only during the reproductive phase of the crop contrary to the views of Setiyono et al. (2007) and others that photoperiod modifies the whole plant development rate. The photoperiod and temperature effects were observed to be that of sensitivity and not accumulation. The accumulation concept (rate of development) used by Setiyono et al. (2007) did not capture supra-optimal photoperiod response routines and therefore, observations had to be limited to the sub- or near-optimal photoperiod range. Secondly, their development rate used mean photoperiod values of pre-flowering stage (a long development stage) whereas this study used a fortnight time step (one development divided into several stages). The sensitivity concept used in this growth rate study was able to capture the supra-optimal photoperiod values for each landrace (Oseghale et al., 2017)
Photoperiod sensitivity appears to be essential to delay flowering (through subtractive effect on temperature Oseghale et al., 2017) sufficiently enough to allow adequate biomass accumulation. This seems to be the reason why bambara groundnut, guinea corn, pigeon pea and soybean produced longer vegetative growth when sown in June closer to (longer days) and less vegetative growth when sown in September (shorter days) in latitude 6o N of equator regardless of whether they. are long or short duration types (early or late maturing types). Roberts et al. (1999) has also observed that sorghum landraces grown in Nigeria always flower at approximately the same calendar time (i.e. homeostasis of flowering date). Yet the proponent of accumulation photothermal theory assumes dry matter partitioning depends mainly on temperature and is not directly influenced by photoperiod. And therefore, considers temperature as the most important factor in crop adaptation to severely unpredictable weather patterns but failed to sufficiently acknowledge the colossal impart of photoperiod on floral growth and crop adaptation.
This study also found a robust predictive capacity of photothermal growth rate model across ranges of both cultivars and environments. Hence it is considered the most appropriate model for simulating management x genotype scenarios in environments varying with photoperiod and temperature.
The current use of growth rate in this study rather than development rate explains well the qualitative short-day response. We considered there to be sufficient evidence to justify a re-evaluation of the growth rate for development rate in evaluating effect of photoperiod on photoperiod sensitive crops. Early flowering or precocity does not always determine optimum yield. Precocity is not a yard stick for measuring yield, but the abundance of flowers and pods is. Timing of optimum flower/pod growth rate determines optimum yield per plant; hence the expression of optimum flower/pod/fruit growth rate is an expression of optimum crop yield and therefore most appropriate for simulating likely consequences of alternative crop management cultivar choice strategies.
Qualitative photoperiod sensitivity provides a mechanism that contributes to the homeostasis of flowering/podding at a given location that is independent of variable sowing dates (Roberts et al., 1996). Tropical farmers know that some crops (e.g. indigenous sorghum, pigeon pea) have qualitative short-day photoperiod response from their experience. How can they be convinced through agricultural decision support system that these crops are quantitative in response? Bearing in mind that these farmers will have to transform facts and figures into practices.
Relevant answer
Answer
Mr. Oseghale , the cardinal temperature regularly calculate from the intersection of the two linear regression lines of growth rate at sup optimum and super optimum temperatures . The optimum calculated from the mixm growth related to temperature .
The development can divided to many stages for example pollination , fruit set , colour itc.
  • asked a question related to Columbidae
Question
5 answers
what is the type of salmonella cause salmonellosis in pigeons?
Relevant answer
Answer
Hi,
More species of salmonella such as Salmonella enterica subsp. enterica serovar Typhimurium.
Look in attach file
  • asked a question related to Columbidae
Question
2 answers
Can someone suggest me a website which can generate PigeonCad images from the pigeon code (synthetic biology language)? I made my code and pictures with the PigeonCad online tool but the http://pigeon.synbiotools.org site is not allowing me to create high resolution pictures which would be sufficient for publication.
Relevant answer
Answer
I suggest you try DNAplotlib from the Gorochowsky Lab - they wrote a good introduction to it and you get much more features than with PigeonCad.
  • asked a question related to Columbidae
Question
5 answers
What happened to this pigeon's wing feathers?
Is it a genetically problem? Or a dietary deficiency?
What is your suggestion for treatment?
-It's sisters and brothers show this appearance too.
Relevant answer
Answer
Check for Ragged condition, which is genetical.
  • asked a question related to Columbidae
Question
1 answer
Found at a home which pigeons live near the windows
Relevant answer
Answer
Argasidae or soft tick.
Looks to be Otobius megnini. But there are many types of soft ticks.
  • asked a question related to Columbidae
Question
1 answer
Dear Fruit-dove biologists, I try to gather data for a Population Viability Analysis of Ptilinopus coralensis. Do you have such data: maximum age reproduction, sexual maturity, mortality %, etc. for other Ptilinopus sp. This is for a conservation project. Thanks for your knowledge!
Relevant answer
Answer
or from other pigeons, doves, etc. I realize that nothing is available for fruit-doves.
  • asked a question related to Columbidae
Question
6 answers
Does anyone know of a good phylogeny for the columbiformes? Most work seems to be at finer scales within the order.
Relevant answer
Answer
Yup, all of those, and  a couple more:
Heupink et al. BMC Evolutionary Biology 2014, 14:136
Cibois et al. Molecular Phylogenetics and Evolution 70 (2014) 442–453.
But somehow OneZoom still seems to be the most complete - maybe Lapiedra et al 2013 is to be used if one needs a dedicated phylogeny.