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i am synthesizing the anti-corrosion coating DLC. i need to know the difference of using these tests to study the properties of the coat. for instance, the XPS just tests the surface of the coat and can illustrate the elemental and bonding properties of the surface of the coating. so what about the others
I investigated the corrosion resistance of Mg alloys with the PEO coating. When I compared the results from electrochemical impedance spectroscopy (EIS), namely Rp values were much higher compared to the Rn (noise resistance) obtained from Electrochemical noise (EN) measurements. Could you help me to explain this phenomenom because literature says these characteristics should be in correlation.
Thanks for any comments.
I have 3 substrates
p-si (0.001-0.01 ohm cm), n-si (1-10 ohm cm) and glass.
When I use si wafer as a substrate during RF sputtering I have noticed that the edges are not coated and coating is near the center part of the substrate. I cannot give any tape for thickness measurement also. Will this problem be in case of glass substrates too. Is it possible to bias a glass substrate?
I ran the single cell PEM fuel cell (Air-H). I employed stainless steel (uncoated) bipolarplate (BPP). basically, the conductivity is less than normal metal BPP. The galvanostat EIS test was run on this fuel cell. finally I saw the results like the attached image. the 3 EIS tests was run in AC 1A, 0.1 A and 0.01 A. the style of all are the same. Also, I ran EIS test with gold coated BPP and the result was normal (2 semi circles). I am sure about the connection and setup of test and fuel cell.
We use 990 ul of antibody solution and then add 0.4mg EDC and 1.1.mg sulfo-NHS (in MES) and react for 15 minutes at room temperature, before adding it to the amine surface for immobilization. Now when we are looking to scale this process to coat tens and hundreds of units for clinical studies, we are unable to follow this process due to manual labour required for each step, and are trying to automate it at lab scale.
Firstly I wanted to know how long can this solution provide the same performance at Room temperature? Secondly, how can we increase the shelf life for this to longer period (at least 30-45 minutes) to ensure high precision and repeatability? We don't have budget for a fluid injection and dosing system for automation.
I want to know if the OTS comes in solid or liquid state and also how to make the OTS solution, which solvent to use for the desired purpose.
I want to coat APTES on pHEMA thin film. I have prepared a Silanization solution (5% APTES in 95% EtOH) and the pH was adjusted to 5 by acetic acid. But it is not work. Can anyone help me provide some suggestions?
I am planning to work with acidic piranha solution made from sulphuric acid 98% and hydrogen peroxide 30% (volumetric ratio 3:7).
I worked with this solution before, but this time I want to use it for cleaning of photolithographic masks (soda-lime glass with chrome layer), which is a standard oparation in photomask cleaning.
Standard usage on piranha etch comprise teflon holder to hold/support mask/wafer/sample. But in some applications tweezers could also be desirable (single wafers instead of batches, small volumes of piranha solution).
My question is: which type of tweezers should I choose for this job (what material), especially for operating large surfaces like wafers or lithographic masks? I did not find consistient and comprehensive info about it.
Teflon tweezers seems sturdy and uneasy to use (manipulating glass mask).
Stainless steel could be susceptible for corrosion. Has anyone had experience with it?
Stainless steel coated with PTFE seems viable, but what about scratching PTFE and exposing stainless steel to corrosion?
Maybe some other materials could be used for the job?
I will appreciate any feedback.
What is the best way to remove the residue of an alginate gel from a production line which is used to coat sauages?
Since the residue is very stubborn, which chemicals would work best to remove this gel-like substance?
Looking forward to cleaning suggestions!
Synthesis of tio2 and sio2 nanoparticles using sol gel method, what should be the ideal PH?
Most of the articles do not write how much PH should be.
i want to do stainless steel coating by tio2 and sio2 nanoparticles for air treatment
Hi, my name is Bram Wolters and I'm a student of the University of Applied Sciences Arnhem and Nijmegen in the Netherlands. I'm working on a project to make sustainable disposable items for the foodservice industry. The disposable items are made of reed, include forks, knives, spoons, cups, hamburger boxes, bowls etc. We want to pulp it, shape it, cut it and dry it. Only I don't really know which binding material and coating to use. I believe it is possible to use cellulose, but have no expert to confirm. Can someone help me?
We were measuring Cassiterite samples on our Jeol FEG-EPMA (15kV, 15nA, focussed beam) - using a cassiterite primary Std to standardize Sn. On the unknowns (also cassiterite), we got very high totals of 107-108%... Any ideas what might cause this?
The Nb-Ta oxides we analysed on the same routine gave good results.
Porous primary cassiterite std?
Bad polishing/carbon coating of the Std holder?
Something to do with the matrix corrections (ZAF)?
Suggestions/tips much appreciated.
I am currently working on FE simulation of cold spray with TiN coated using Johnson-Cook material . However, I've been unable to find the JC values. Can anyone provide these values?
I am having trouble sputter coating Mg on glass substrate. The adhesion is terrible during lift-off and I lose significant yield. I tested the same process with a silicon wafer, and the results were extraordinary but terrible with glass.
I know I can use an adhesion layer like titanium, but at this stage, I want pure magnesium structures.
Is there anyone with experience regarding this problem?
we coated titanium gold by sputtering method, recently the surface is very bad, maybe it's because the surface is not clean? soiled?
Hello. Thank you for reading my question.
Now, I need hydrophilic wire for my experiment system.
So I consider aluminium wire and plan to coat it hydrophilic.
I would like to ask you how to hydrophilic coat the Aluminium wire surface.
I would like to make contact angle below 45 degrees.
Thank you for reading.
Hello, I would like to do dip coating for MOF material on fiber, cellulose and so on.
MOF is kind of very fine power, and It was very difficult to do coating.
Could you advise ? and Any coating idea ?
Ex Situ or in Situ growing seems to be cost inefficient.
Dip Coating will be simple and cheapest way to coating.
Any interaction idea btw substrate and MOF ?
Binder can be block the pore ?
Hello, everyone. I want to process a metal mirror. This type of mirror is used at 60kPa, 370 degrees Celsius with up to 200ppm concentration of SO3 gas.
I currently have three options for making this mirror. One method is to use aluminum alloy as a substrate and gold as a coating. Another method is to use copper as a base material and gold as a coating. The third method is to use copper as the base material, chrome as the intermediate coating, and gold as the outer coating. I wonder which one is better for long-term use? Besides, what better way than these three? thank you
May some of you performed staining for immunofluorescence of PBMCs in 96-well plate? As the cells are not going to be cultured, only stained in these plates for confocal microscopy, we are planning to centrifuge (600 g, RT, 6 min) cells in 96 well plate to attach them to the surface of the plate, however I am not sure if this is enough to attach them. I know there are coating plates, but for decreasing costs we are searching alternatives.
Where should I start?
What solvents can I use for extraction?
What methods should I use to identify them?
What method should I use to determine their molecular structures?
Does the coating thickness affect the XRD peak intensity?
I am growing astrocytes from the striatum of adult (70 days) male Sprague Dawley rats. I use laminin+poly-D-lysine to coat T75 flasks on which the astrocytes could attach. Of late, the astrocytes are unable to adhere to the flask surface and are dying off. I have tried using a new coating solution, and new plates but nothing seems to help.
Could anyone please help!!
I am trying to coat a film of Barium and Zirconium precursors over a glass but I get this type of circles (see picture attached), and the film does not seems to be uniform.
Recently, after each coating, the plating cavity appears blue purple, we use Agron and N2 to cover the yellow color.
These publications indicate that the logarithm of the metal concentration in the electrolyte solution, depending on the logarithm of the metal concentration in the coating during electrodeposition, is linear, which indicates a mechanical mixture in the coating (copper and zinc separately, not brass). I did not find any relevant data in foreign sources, do you have any information on this?
1) Рыбин А.А. Электроосаждение сплава олово-индий из сульфатных
электролитов с органическими добавками: диссертация кандидата технических,
наук, Москва, 2015.
2) Валеева А.Х., Валеев И.Ш. Электроосаждение сплава SnSbCu на медь
из электролита с различным содержанием хлорида сурьмы // Известия высших
учебных заведений. Физика. - 2015. - Т. 58. - № 6. - С. 121-124.
3) Федотьев Н.П., Бибиков Н.Н., Вячеславов П.М., Грихилеc С.Я.
Электролитические сплавы. М.–Л.: 1962. 312 c.
I took SEM pictures of my clean 0.2um pore size PES membrane filter. The first picture is coated with 5nm gold, and the second is with 20 nm carbon. Both pictures are 2500X magnification, and the scale is 4um. I wonder why the two pictures look significantly different. The picture I coated with gold doesn't look like the pore size is 0.2um.
I want to perform coating of PMMA-ceramic composite on metallic substrate. but the coating gets detached after it is completely dry. can I add any adhesive to get proper adhesion? kindly suggest?
You can think coating adhesive and then producing packaging tape with the BOPP Film.
I am trying to validate DNA Aptamer-cell (Bacterial cell) interaction in vitro with Biotin-streptavidin assay.
- DNA aptamer (41 bp) is labelled with Biotin on 3' end. After incubating aptamer with whole bacterial cell, I am trying to separate aptamer bound cells using Streptavidin coated magnetic beads.
- Then Isolating DNA of the separated cells and gene specific PCR to validate the specific binding of bacterial cells to aptamer.
- But I am facing few hurdles meanwhile. Would love to discuss with expert in the field of Aptamers.
Kindly share and help me connect with expert.
What are the Scale dependent & non dependent parameters in Coating process ?
We are finding a hard time to strip of residual HSQ ( Fox 16) after using it as a mask in etching? We are coating 600 nm thick HSQ and writing with 3000 microC/cm^2 dose at 1 nA current. We are developing in 25% TMAH and after development process we can strip off it easily by dipping it to 6:1 BOE ( buffered oxide etchant ). However, when we are doing argon sputtering in oxford etcher and using developed HSQ as a mask layer, we are unable to remove residual HSQ even if we dipped into 6:1 BOE for 25 mins. Your guidance will be highly appreciated. Thanks
1) For conformal coating on electronics, what will happen if there is a very thin coating of say only 1-5 micrometers with process point of view?
2) Will this solve the purpose of environmental protection and basic insulation?
3) Will this thin layer be fragile to be handled during processing?
4) Will there be any fouling of this layer during its application?
I have recently started working with mammalian cell lines, especially HepG2 cells for which I'm culturing cells in Nunc coated T25 flasks(Cat No. 156397) with 89% DMEM, 10%FBS & 1% Pen Strep.
The media is changed every 3rd day and cells are split once reached 70-80% confluency at split ratio 1:3.
Before, trypsinization, I washed cells twice with 1X PBS.
I earlier used 0.05% Trypsin EDTA for detaching cells by keeping it at 37C for 5 minutes but a maximum of 70% of cells did not detach.
I then used 0.25% Trypsin-EDTA solution and kept it at 37C for 15 minutes and tapped the flask vigorously, still, around 50% cells did not detach.
Kindly help me with this problem.
Thanks in advance.
Magnetic decantation was tried but the particles were found to be present in the supernatant. Centrifugations did not work. Trying to go with dialysis (using haemodialysers) and looking for the right pump to use.
We are using SiO2/TiO2 as materials for AR coating. Due to the limit from the tool, the TiO2 index is always lower than target.
I am wondering to switch to another low index material, MgF2 seems popular. What would be the advantages and disadvantages of switch from SiO2 to MgF2?
Thanks a lot.
I ask suggestions for differentiation medium and differentiation days. In addition, the possible use of matrices and coating.
I am trying to coat conducting materials like graphite, graphene, graphene oxide on glassy carbon electrode, but the coating is unstable and uniform. I have been using PVDF as binding agent and i have tried various solvents to disperse GO, rGO and graphite.
The coating is very fragile and some times get removed easily.
Need some expert opinion. Electrolyte is 1M KOH
I'm curious to see if anyone has experience using Sylgard or Parafilm to coat their pipettes when doing whole-cell voltage clamp. I found that this technique has frequently been present since the late 90s on voltage clamp papers, and I'm wondering if it's worth doing to reduce capacitance on my own cells. I am patching on Purkinje neurons in both dissociation and slice preparations, and recording INa current.
If someone could point me to a paper or just describe their experiences here it would be greatly appreciated! I'm not exactly sure where to start (how much to add? where to add? what techniques work best? do you apply either under a dissection scope?) so please be descriptive as possible.
ich try to figur out how Formula A) [PICTURE 1] has come about.
I try to messure the water uptake of coatings and found this formula without context.
I tryed formula B) [PICTURE 2] and came to nearly the same results. (the understandable formula)
When i have the capacity at 10 kHz (according to the formula) i go with it in Formula 3) [PICTURE 3] [Brasher–Kingsbury] and get my water uptake of the coating.
Why do you use a coating buffer containing carbonate / b-carbonate for ELISA?
Do you use a combination of carbonate and bi-carbonate?
Or do you just use one of them? If yes, when do you use carbonate and when b-carbonate?
I have synthesized carbon coated material. however when I performed TGA, there is weight gain in my bare sample and weight loss in carbon coated sample?
We need collagen IV coated plates in order to culture primary queratinocytes. However, the usual supplier of this kind of material (Corning) is not supplying it until, at least, 2024. Does anyone know any other supplier that could serve these products as soon as possible in Spain?
I want to isolate the macrophage cell membrane and then coated it on nanoparticles. There are many protocols for the isolation of cell membrane vesicles, but I want to separate the macrophage cell membrane and next prepare cell membrane cracks. In addition, protocols suggest different hypotonic lysis buffers for macrophage cell membrane isolation (20 mM Tris-HCl, 10 mM KCl, 2 mM MgCl2, and 1 EDTA-free mini protease inhibitor tablet or 1 mmol L −1 NaHCO3, 0.2 mmol L −1 EDTA and 1 mmol L −1 PMSF or Tris-magnesium buffer (TM buffer, pH 7.4, 0.01 M Tris and 0.001 M MgCl2), which I don’t know the best one. After cell lysis, the protocols are followed by sucrose gradient or differential centrifugation, or sonication. I am worried about confirming the cell membrane after isolation and the best protocol for membrane isolation and saving them besides coating the nanoparticles with macrophage cell membrane cracks.
Any help is appreciated!
Suppose we have two combined structures that are conductive but not electrically connected to each other (please check the figure). Is there any way we can selectively coat only one structure with a polymer? Or coat one structure with one polymer while coating the other structure with another polymer?
I am radiolabeling silica nanoparticles with a certain radioistope, however after purification I found that the activity was still in the eppendorfs, means that my particles get stuck to the tubes, although I am using low adhesion eppendorfs.
So what is the best way to prevent adhesion ? coating with hydrophilic polymers to polysterens ? and how to do so ?
I am testing various coating concentration of our recombinant antibody in a direct sandwich ELISA. I am testing coating concentrations of 75, 150, and 300 ng/mL with a standard (His-tagged protein) and human serum (containing native protein) diluted 1:10 in PBS. When the coating concentration is increased from 75 ng/mL to 150 ng/mL, the optical density measurements increase by about 14% for the recombinant protein standard, yet for the serum samples the optical density readings double. Moving from a coating concentration of 150 ng/mL to 300 ng/mL, the optical density measurements increase by about 5% for the recombinant protein standard while they increase by 50% for the serum protein.
When I coated copper foil with anode slurry, the surface seemed uneven, and tiny grains were seen. like the following image. I can't understand why. I tried to solve this problem (for example by increasing the time of mixing), but the methods didn't work.
Thanks for any suggestion
In the fabrication of solar cell can i use antireflection coating nanowire as a transparent conductive oxide ?
I recently performed an experiment where I coated a CD3 antibody on a 96 well (flat-bottom, TC treated) plate at a concentration of 4 µg/ml (50 µl per well) for 2h at 37°C, 5% CO2. Afterwards, I rinse my plate 1x with PBS and plate my cells asap.
This is something that I do routinely, and my specific type of cells always reacts in a very standard/comparable way (strong activation together with CD28 4 µg/ml). However, this time my cells were not activated properly despite coating my plate with CD3 and adding CD28 in the culture media.
The only difference between now and previous times is that after coating my plate with CD3 for 2h, I rinsed my wells and then left my plate with the coating for another 2-3h in the flow without any fluid in the wells. Extra info: the antibody is not expired and all other steps were performed exactly as in previous experiments (no temperature variations, same incubation time ect.)
Could this explain why my cells were not properly activated? And does anybody have experience with storing a CD3-coated plate for a longer period of time?
Thank you in advance for all input.
Some professional painting contractors apply primer coating in a building wall. After that they apply top coating even after delaying 4 to 5 months of primer coating. If it is delayed such a long time will the top coat adhere properly on primer coating surface? In such process will the longevity of top coating not be affected?
As I know, metal oxide materials(Al2O3, WO3 ....) have very low reactivity.
Also they have high melting point.
If we calcine those metal oxide with cathode material(NCM) under 500℃, it will not be happen chemical reaction between NCM and metal oxide dopant.
Is it right?
If it is true, how can NCM be coated by metal oxide dopants? Just physical coating by surface energy?
In that case, is high surface energy of NCM advantages for better surface coating?
If not, please explain well-known mechanism with reference :)
I hope your happy day!
I have been culturing human astrocytes derived from brain samples. Do not have any problem growing them on T25, T75, and multi-well plates (Poly D lysine coated). But, when I grow them on coverslips coated with PDL, astrocytes detach and I am barely left with any cells on the coverslips. This happened many times despite troubleshooting. Astrocytes adhere very nicely on coverslips coated with PDL, but they detach easily after PFA fixation and washes with PBS. All the steps have been performed very gently to avoid cell detachment. But nothing worked. So, I seeded human astrocytes directly on the chambered slides (without PDL coating), and I faced the same problem. I couldn't figure out where the problem is. I checked many papers that mentioned growing primary mouse and human astrocytes on PDL-coated coverslips.
I request experts who worked on human astrocytes to kindly suggest what I should do.
I would like to immobilize fungal cells (Candida and Saccharomyces cerevisiae) onto the surface of glass beads in order to use them, after the immobilization, as the inoculum to obtain a liquid colture.
I have found a procedure that uses Polyethylenimine (PEI), that seems to be what I am looking for but I can't find any information on the kind of PEI that is used.
Here you can find the article that proposed this procedure in the first place (1986) where they used "0.2% PEI solution". In this paper no specifics were given on the kind of PEI used and papers published in the following years were not specific on the PEI used either.
I am not familiar with PEI and I don't really know what I should look for and there seems to be a whole lot of different options:
Can anyone help?
I would like to know the method of spheroid formation using PC-3 (prostate cancer cell lines)
I could not achieve the super hydrophobicity with epoxy/ ZnO coating, even after treating with stearic acid. I am looking for insight.
I am trying to coat gold nanostars with mesoporous silica shell but many AuNSs are coated with silica instead of one individual nanostar. Anyone has any idea why and what I can do to have one nanostar coated with silica.
Is the sol-gel coating formed on a carbon steel surface by dip coating its coupon in a chemical bath of HfCl4+ ionic surfactant solution followed by heating at 550 degrees celsius can be named Hf conversion coating on a carbon steel surface?
I would like to make a collagen I coating.
Is it possible to adopte directly a buffer solution to dissolve collagen powder reaching a specific concentration?
Or do I necessarily have to use an acid, such as acetic or hydrochloric, first and then dilute it with buffer?
Thank you in advance.
If anybody has prepared a coating buffer before, please help me with these 3 queries.
1. What method do you use for sterilization of coating buffer?
2. Can you store the coating buffer at room temperature?
3. What is the shelf life of self-prepared coating buffers.
In the case of having hydrophobic iron oxide nanoparticles coated with oleic acid, what happens when conducting the phase transfer process in order to add a polymer (PEG) on its surface? Does the oleic acid remain on the surface of the IONPs or does the PEG take its place? And what kind of chemical interactions happen there (how is the bonding between the chemical compounds made) ?
Thank you for your time and answers!
Based from your experience, I would like to ask if it is usual that there are dead and floating transfected HEK293 cells after seeding in PLK-coated coverslip?
In my experiments, I used 0.025 mg/mL of PLK to coat a 12 mm coverslip in a 24-well plate. Then, I washed it 4 times. Then, in my seeding experiments, I made sure that (1) I used a warm DMEM medium, (2) gently shake the seeded cells, and (3) minimize the bubble formation during resuspension.
I thought this would reduce the number of dead floating cells, but I still got dead and floating cells that aggregate in the middle. Nonetheless, I still got healthy cells that were attached in the coverslip.
Hoping for your insightful answers.
I have prepared the material for the removal of heavy metals from the wastewater. Now, I want to test it over the filter. How can I coat the material on the filter? Either should I dip the filter into the prepared material or pour it on the filter paper?
I'm trying to simulate a shear tensile test in abaqus by using a single lap joint speciment, and couldn't figure out a proper way to put a lair of coating on the adherends. I've tried the skin method to add the coating lair, but somehow the simulation result turned out as the image is shown, which seem the adhesive lair deformed in an abnormal way.
Can someone teach me how to properly use the skin, and also tell me why the epoxy lair deformed in such way?
I am trying to coat PEDOT:PSS on plastics but it is very difficult to put the solution in plastics (thickness vs binder). What should I add to this part?
hi everyone, I am working on differentiation of fibroblasts to iPSCs. I am planning to. use matrigel coated plates, but am a little lost on what concentration I should use. I am using Corning matrigel (REF 356234). The batch I have has a protein concentration of 8.9 mg/mL. the data sheet just says to not dilute to less than 3 mg/mL. in your experience what isna good concentration to use for this application?
I am currently developing a high hardness coating for PET and the problem now is that the coating can be very hard (pencil hardness 6H), but it is so brittle that it cracks easily when bent.
The main materials I use are urethane acrylate and nano silica sol, and The thickness of UV-cured coating is about 20um. Is there any way to maintain the hardness of the coating while increasing its flexibility ?
Can you give me some advices ?
thank you a lot !
Can anyone please help in suggesting some relevant papers on "polymer-coated hydride particles" that give the product in a powdered form, not a composite?
- Which criteria should be considered in order to distribute the copper thickness homogeneously (uniformly) while copper plating is done by electrolysis?
- We want to coat the copper film as uniformly thick as possible on a flat metal bar. What should we pay attention to?
- What is your suggestion for a book/article about this?
I am currently manufacturing a graphite||LiFePO4 battery using a LiPF6 electrolyte. Both are coated on copper and aluminum foils respectively. I assembled them in a split test cell in inert conditions. As assembled OCV is 0.4 volts. At a charging rate of 0.008 mA, the voltage rises upto 3.6 volts, however, as soon the charging is stopped, voltage falls back to 0.4 volts again.
It would really help if the respective experts guide in this regard.
what influence causes the coating in the electrochemical deposition process to be inhomogeneous
I want to coat a palladium-nickel alloy with a ratio of 92-8. The machine I have access to has an one electron beam and four crucibles.
I have two solutions.
1- Separate coating of palladium and nickel and then annealing in vacuum.
2- Placing palladium and nickel in a crucible and coating palladium-nickel alloy.
Which do you think is more suitable?
Is there any other solution?
I tried to use PVDF as a binder for my material but it did not dissolve well but it can be dissolved in PTFE but i can not understand the ratio's as PTFE is thick binder and the consistency of the end product is very thick and cant be coated on foil.
To check on oil based coating on plastic sheet