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New transplant programme starting in a small African nation, but they will need support and training in the examination of biopsies. A digital 'whole slide' scanner would allow virtual biopsy images to be shared with expertise in the UK. If anyone knows of a machine not being used and willing to donate, please get in touch!
Best wishes,
Benedict Phillips
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نعم يوجد
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Which one has the biggest impact on the diagnosis of diseases, pathology or medical laboratory science?
Who knows more details on the diagnosis?
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Medical laboratory science involves running clinical tests on patient samples. This requires a 2 or 4 year degree. While medical laboratory scientist play a key role in providing information for diagnosis, we do not actually diagnose people. A pathologist holds a medical degree, and thus would be more involved in making a diagnosis.
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In my lectin haemaglutination assay by doing 2-fold serial dilution I have to add 50ul of trypsinized rabbit erythrocytes instead of normal rabbit erythrocytes and also I have heard that the wells need to be coated with EDTA. Can we do the EDTA coating ourselves? Please explain. Thank you. Also can we use direct human blood for the assay. If yes then how to store it till we do the assay?
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Hi did you find any answer for your question?
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I can not get back to grow up my MDA-MB-436 cells after freezing in liquid nitrogen tank.I used 7% DMSO with FBS for freezing medium. But when i do thawing again my cells, they didnot alive again.
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My advisor advise to me to use 7% DMSO, the problem occured only in MDA-MB-436 cell lines only , so I have idea to change the DMSO % from 7% to 10%, Thanks for your Suggestion Sister Nguyen Tu,,,,, Dear Magdalena Matysiak,,I cant solve this problem.
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We are looking for ways to support our novice preceptors, whether it be through education, administrative support, or other factors.
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Mentoring novice preceptors by an experienced clinical preceptor with in-depth clinical teaching and evaluation.
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I have tried digitonin, but seems it also break mitochondria outer membrane. Any one has better suggestions?
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I do not know exactly what are your intentions in this matter.
- If mitochondria are to be used for "proteomics" then consider mechanical cell breaking methods and differential centrifugation. Be aware that the yield and purity are always "disappointing".
- Now if you intend to deal with mitochondrial function and plan to access to mitochondria inside cell an alternative to digitonin are permeabilizing toxins, I know that "Seahorse" commercializes some kit about that. But getting back to digitonin:
Too much digitonin solubilizes everything, mitochondrial inner membrane is relatively resistant to digitonin because of low/no cholesterol is present. The situation of outer membrane is different. Digitonin has been used in the past to prepare "mitoplasts" which are "mitochondria" that have lost their outer membrane.
Now how to detemine the concentration of digitonin that would permeabilize plasma membrane and respect the mitochondrial inner membrane? A possible strategy is the following: Measure respiratory activity of cells (oxygen consumption) in a medium compatible with "isolated mitochondria". 
1) Add rotenone ≤1µM in this respiring preparation, cellular respiration should stop.
2) Now add succinate in the medium, little/nothing should happens as succinate is not permeant through a normal/healthy plasma membrane. If respiration resumes it reveal the presence of cells with a damaged membrane.
Note that you may invert 1 and 2 (succinate first).
3) Add gradually digitonin, and when respiration resumes this means that succinate can get in and thus plasma membrane is made permeable to small molecules. As far as I remember with a # 10e6 cell / ml suspension this took place with concentrations of digitonin in the 5-50 µM range.
Note that  the  stability of this respiratory rate is likely to be dependent on the presence of both ADP and Phosphate in the medium as intracellular small molecules are expected to leak out and to dilute in the large extracellular volume.
Good luck with your exps.
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Where can we get the plastic nozzole spray which can be fitted onto syringe head for clinical application of cells
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you can get that spray in good price from this well-known shopping site Alibaba and here is their link: http://www.alibaba.com/showroom/plastic-spray-nozzles.html
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i have to set a group of positive control with DP vaccine, i find it but now i face with a big problem!! the unit of this vaccine set on LF (flocculation) , and I don't know how can I change it to mg/ml and actually this control group is too important for me to show my result and compare with it.
i read papers and protocols a lot but i found that there is no coefficient to convert it, because its not constant.
I thank you to show me solution. its really necessary. my mice is going to be older and older.
Regard.
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4. CONTENTS
Country of origin of biological material: Denmark.
Liquid tetanus toxoid, non-adsorbed, was donated to NIBSC in June 2004
by Statens Serum Institute (SSI), Copenhagen, Denmark. 1 ml of toxoid
per ampoule was freeze-dried at NIBSC in November 2004, with a total of
5,900 ampoules prepared and 5,856 available for use. The material is a
purified tetanus toxoid (of purity > 1000 Lf/mg pN) stabilised with
trehalose. The average weight of the ampoule content was determined as
0.026 g of dry weight +/- 1.0%. Mean residual moisture content was
determined as 0.92%. Each ampoule contains 0.1M NaCl and 1%
trehalose.
so 1 LF  = 26 mg
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Can anyone please share their lab protocol when handling Adenovirus experiments. I need to do one experiment using adenovirus and wanted to know If that would contaminate the hood and incubator, can I use a common hood and incubator or that would contaminate other cells. Thank you
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You are welcome Ola
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I have already a polyethylene lid which is not efficient. I tried to use parafilm but the solvent still evaporates. I tried to put Aluminum foil then the lid on to make it a quick fit, but the solvent still evaporates.
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The teflon stopper is a best solution with the conventional cells on the market. However, take care about the change of the volume as result of the temperature change. In a large temperature range it can influence the concentration substantially.
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I need information about the right amount of the reagents and sample
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I have sent you a link where you can find materials and method to measure IMA.. we have published paper on it.. Hope it will help you to measure with less cost..
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I want to use Tetanus toxoid as positive control for my allergen experiment. But I need the concentration in micrograms/ml. What I have found in our stock is Lf or flocculation limit. Can anyone help me solving the matter of how to convert this Lf to micrograms/ml?
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And some additional material also at: