Science topic
Citrus sinensis - Science topic
Citrus sinensis is a plant species of the genus CITRUS, family RUTACEAE that provides the familiar orange fruit which is also a source of orange oil.
Questions related to Citrus sinensis
We do not intend to dry the peel as we assume some components might be lost.
I am showing two curves here, both reaching convergence. Blue (force constant of 250 kJ/mol/nm and velocity 0.01 nm/ps) and orange (force constant of 500 kJ/mol/nm and velocity 0.05 nm/ps). To the best of my knowledge, it is preferable to go for the lowest values because we don't want to perturb the system too much. But with the blue plot, it is not smooth. I do see a small plateau/microstate. I don't know if that's ok.

We are having a big problem of citrus decline problem.
I am trying to investigate the thermal stability of a protein I work with using the TSA with SYPRO Orange. However, even though my protein produces a peak at about 43 degrees, it shows very high fluorescence at lower temperatures. What could be the possible reason for this high background? How can I optimize the assay?
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable, because the scale is numerical and is basically a value for pigmentation (making it a numerical variable) or if it is ordinal, because the score suggests some sort of order. Can anyone help?
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a contamination (for example mold spores)?
Thank you in advance for your help!


First titanium nanoparticles were made through green synthesis.
To perform photocatalysis of methyl orange dye, different light sources were used.
We added 0.05g(50mg) of TiO2 nanoparticles, 0.5 ml hydrogen peroxide to a 50ml solution of methyl orange in a set of two beakers, stirred in the dark for half an hour to achieve the absorption - dis equilibrium.
One beaker was kept under the UV light of biosafety cabinet since we do not have a photoreactor. But after 3 hours of irradiation, it did not degrade the dye.
The other beaker was kept under tungsten bulb as the light source with a distance of 5 cm on a constant stirrer for 3 hours.
But after 3 hours of exposure to light there was no sign of degradation.
We had balance the pH also by adding HCL dropwise to make the methyl orange dye solution acidic (4-5 pH).
The experiment does not yield result in either of the above mentioned light sources. What are the factors that need to be changed or are missing for dye degradation to occur.
What are the merits of using Methyl Orange rather than other Dyes in photocatalytic degradation?
We´ve been using the Picro Sirius Red staining for several years now (done in out core facility) to show fibrosis development.
However suddenly our stainings, which usually had a yellow background turned orange, which makes the red fibers much harder to adequately quantify.
Our core facility hasn´t strayed from the usual protocol and no solutions were replaced or anything done differently than before, at least according to them.
We´ve done several stainings by hand, all of which have come out orange.
Does anyone have any idea how exactly this might have happened or how to make our background lighter yellowish as it was before?
I want to stain acute brain slices with calcium orange and DAPI, but I am not sure whether these two kinds of dye can be added together or not.
I have a recipe of loading dye (10 mM EDTA, 1X TBE, pinch of Xylene cynol and Orange G dye which is made upto 10 ml with 100% Formamide) but somehow the bands of the nucleotide product (enzymatically degraded DNA) are not distinct and well defined or fuzzy. I use 1 X TBE buffer (freshly prepared), the volt used to run the gel is 1500-2000 Volt or 25-40 W. Could you please suggest the recipe of loading dye or other factors that could give crisp bands for publication purpose. Any advice would be highly appreciated.
Thank you in advance
Prem
What kind of chemical is used as an anti-mold agent in orange wrapping paper?
Need this for my research please also indicate which article it was from, thank you.
I am doing comet assay for gills of fish, the fish were exposed to pesticides primarily.The problem i am facing that the cells are not picking stain.Can anyone tell that while using acrydine orange in order to stain DNA of gills.which concentration we should use?
In September this year, I noticed an orange/green coloured scum layer in a slow-running river (see pictures) close to my hometown (Deinze, Belgium) containing green and orange clusters, as can be seen at the close-range picture of the bloom.
Microscopical analysis revealed a micture of Microcystis-colonies (a bloom-forming cyanobacterium, the green part of the scum layer) and an unknown organism of which I have no clue what it could be. I encountered a similar (mixed) bloom about 10 years ago in a recreative lake in Flanders (see report-snippet in attachment).
It might be some micro-algae, or spores/pollen (but in this season?) infected by an aquatic fungus, or both structures (cells and what looks like hyphae) might concern the same organism (a fungus?). I however doubt it concerns a micro-algae.
Since than, I received more records of such scum layers from the nearby river Scheldt, and I personally saw something quite similar in the river Moldau at Prague three weeks ago. I hope someone familiar with this organism can tell me what it is?
Many thanks!
Jeroen





+3
Anyone knows exact dye name with code and other information. Reactive orange with 418 nm wavelength.?
I have prepared an azo dye with orange colour in polar solvents. As is seen in the absorption spectra, absorption is in the UV region and up to around 400 nm in the visible. However, an orange colour solution typically shows absorbance in the 500-600 nm range. This dye also shows a green specular reflectance in a dry solid form. Could anyone please explain the possible reasons for these two phenomena?
What is the best dye to use for Automated Liquid Handling System dispensing verification? Currently i am using Orange G. but if you have a good data with other dyes, please share it with me. Thank you.
hello evryone,
when a polymer has a narrow( fig below) distribution, the macromolecules don't flow in the same way, so this will impact the polymer's viscosity, which can lead to extrusion distortion.
On the other hand, if the polymer's distribution is wide( fig below), there is consistency in the viscosity, so the orange skin ( fig below) effect is hardly noticeable. is it true ?


jurnal hukum adat mengenai pembagian harta warisan menurut prespektiv islam kelompok saya terdiri dari 2 orang yakni:
raihan ramadhan hayatuddin (2210611085)
rasendrya hendarwin (2210611386)
I know that both Orange G and Bromophenol blue are negatively charged dyes and that Orange G may migrate faster in an agarose gel.
I found a resource that says Orange G can be used in Native-PAGE and in a DNA PAGE but I cannot find any resources that say whether the two dyes are interchangeable in a protein SDS-PAGE.
Any insight would be greatly appreciated.
As the melting point of methyl orange solution is 300-degree cel, I want to use methyl orange solution as a solvent for the synthesis of conducting polymer. Please discuss.
As the question already implies, i made a mistake during restriction enzyme digestion and added the 6x loading dye to the DNA insert fot checking on the gel. 10ul was added to the gel for checking but the other 60ul that was supposed to be used for ligation later on also has the 6x loading dye added to a concentration of 1x. Will this be a problem later? Will the dye be removed during the GeneJet PCR purifications later? How should i proceed? All input is welcome.
Kind regards.
This is CD20 staining (orange) in mice spleen,
Plz notice the weak stain in white circle compare with the brighter stain in other area.
what's the reason?
Hi All, I am using cell mask orange dye to label HEK 293 T cells plasma membrane. I am using 5 ug of dye as final concentration and incubating cells for 10 min at 37•C. I am facing issue with dye internalisation. I did staining before fixation as well as after fixation. In both case All dye internalize. So here is the protocol I am using:
1. Growing cells
2. wash with PBS
3. Stained with cell mask orange for 10 min
4. washing 3 time with PBS
5. Fixation with 4% PFA
6. Washing with PBS.
7. Stored in PBS,Same day Imaging.
Note: Some time I pellet down the cells after second step and then stained the cell suspension. At the end step I just mount the sample on slide.
How can I prevent dye internalization in cell? what are the possible reason of this issue? Please let me know.
For my project, I use MG63 cell line. These cells grew well in the first passage. But after the second passage, there were many dead cells and the culture medium became turbid and powdery (The culture medium placed in the incubator alone, its turbidity did not increase). Also, the culture medium was cultured on agar to determine bacterial contamination but did not show any contamination. In the next days, the attached cells also detached and died. Also, after changing the culture medium, the orange color of the medium quickly changes to pink within an hour (fresh complete medium contains 10% serum, 1% NEAA, 1% L-glu and 1% pen/strp). What is the cause of this cell death and change in color and turbidity of the culture media? Thankful
I am using sypro orange in thermal melt experiments and am seeing a peak at 52C in the no protein controls, only when I do a full plate assay. I have seen that people mention dye binding to plastic is a problem in DSF – what does this background look like? Is it a peak or just high background? I am using Hepes Buffered Saline pH 7.4 and 20X final Sypro in a BioRad Opus384 qPCR machine and their recommended 384 well pcr plates. I get a really strong peak at 52C – is this something that you have seen?
Thanks, any feedback would be greatly appreciated!
Hi all! There is something that always calls my attention when I am working with Eosin Y, an organic dye and a photocatalyst. The color of its solutions depends on the concentration and the solvent. But also, and homogeneous solution in water/tBuOH, for example, is orange within the body but pink on the edge of the solution!
If you have any comments, want to share your experience I would appreciate it.
Thanks in advance!
Leo!
Please somebody tell the absorbance value recorded for all dyes for ppm... Specially for RhB, methyl violet, methylene blue, or methyl orange.. Tell the absorbance value recorded..
Recently i made 5 dilute solution of methyl orange in water, then measured their surface tension. results showed that surface tension increased by increasing the concentration. dose any body know the reason?
So our lab cultures about 2L of ExpiCHO a week in varying volumes from 25mL to 100mL. In the last 2 weeks we have had 4 flasks (all 100mL) show this weird growth behavior. Other transfections from the same day appear normal, and used the same media. All cultures were properly given 1st and 2nd feeds.
I personally did not perform the transfection, so issues involving human error are possible, but it supposedly was performed routinely without problems.
I am unsure as to what could even have happened, and what is exactly going on in the flask. It appears to be aggregation, and the culture looks to have grown since transfection date. Indeed while the one was a more brownish color, but have the indicative "orange" color of a viable culture, as to a pale yellow of a dead culture.



I want to clone eGFP gene in PUC19 plasmid using benchling app. I am trying to add my insert but one of its sticky end is not compatible. Can somebody tell me which enzyme will i choose from the highlighted orange enzymes? need help in the clonning steps. can somebody guide me in this. i have attached the images. The insert has to be added between BamHl and Ecor1. I have already added my backbone. Now i have to add insert.


Hello everyone, I have been trying to grow ZF4 cells we ordered from ATCC. We are having a really hard time. First of all, it will grow really slowly and after some days they will die. I have been using pre-warmed DMEM: F-12 Medium +10% FBS + Pen/strep. Also, I have to change the media frequently. It will change the color to orange in a day or so. Does anyone have a similar experience? How did you fix it?
I'm working with Trichoderma species that are very close, around 97~98% identity, I wanted to know what exactly the colors indicate and what their implications are for each partition that the program presents.


I want to use an agricultural production model to simulate the yield of oranges for different scenarios. However, I can't find a model that contains an orange crop file or a perennial crop that can inspire me. Help me please.
Calibration curve methodology from APHA
We have to PIT TAG adult Epinephelus coioides for identification purposes.
I want the procedure how to isolate probiotic potential lactic acid bacteria from Fruits
I used Alizarin red staining on osteoblasts without a CPC quantification method. Do you think I can quantify the red/orange color with Image J ? How ?
I have added 10-15 % KOH to a 80:20 mix of 2-(2-Ethoxyethoxy)ethanol: 2-Butoxyethanol.
However, after a few hours to a day it starts to go from colorless to yellow to light orange to eventually a deep dark brown.
My questions are:
Why does this happen?
How do I prevent this from happening?

Hi All, I have many breast tissue samples and have stained them with two chromogens (Red and Yellow) and there is co-localization areas (orange) that I am interested to quantify as well. What would be the best approach to quantify these three colors intensity in each sample?
Thank you in advance
I was clearing a cabinet and I have found this plate from November 2021.
It is LB Agar with Neb10 competent cells.
The contaminants seem bright orange.

I transformed E coli JM109 with pMV361 (Kan resistance) and had a negative control (transformed with water) and 10 plates of the bacteria transformed with the plasmid. Those 10 plates have barely 1 or 2 colonies with the phenotype expected for E. coli, but the negaive control has over 7 colonies some of them kind of orange.
I would expect that if the plates were not correctly made, every one of them would be contaminated. But they have what we expected, very little grow, white, round.
Attached the picture of the negative control plate.

I have a regression problem with two objective variables or outputs (named E & r). I made a model for every objective separately. I used Gaussian processes regression.
I obtained prediction for both objectives as can be seen in the attached images (error bar shows standard deviation).
Title of the plots shows R2 & RMSE of prediction. There is a categorical variable in dataset (Mixer) which has two values (50L, 2400L), shown by different colors on the plot.
Next, I calculated R2 & RMSE separately for every Mixer (shown in the legend in the attached images).
As you can see, for objective "E", RMSE of Mixer 2400L (blue color) is less than RMSE of Mixer 50L (orange color). But, its R2 is very low which is surprising for me. I expect that when RMSE is lower, R2 should be higher.
And for objective "r", RMSE of both Mixers are almost similar. But, R2 of Mixer 2400L is much lower.
I have only one assumption about this phenomenon. Reason of low R2 is because of lower No. of samples for Mixer 2400L.
No. of Observations:
- Total : 119
- Mixer 50L : 106
- Mixer 2400L : 13
If you have any idea. please let me know.




I have a multi-objective minimization problem and the final objective function is written as: F = f1 + f2. However, f1 and f2 are the error functions of two different quantities: orientation error (degrees) and position error (meters). I would like to minimize both of them at the same time.
The questions is: how can I properly scale, or make dimensionless, f1 and f2 before computing F in order to make them comparable (not to add oranges with apples)?
The main difficulty is that I do not know a-priori their range of variation (i.e. orientation and position errors). On the contrary, the ranges of variation of the "original" quantities (i.e. orientation and position) are known from the experimental data.
How is this problem commonly solved in the optimization practice?
Thank you,
Marco
below picture is my analysis model. because at corner part, there are many studs inserted, so such partition is needed. however at the orange color part, at first, it is possible to make a sweep mesh, but because of the influence of the previous partitions, it is difficult to make a mesh like picture 2. After do partition After the partition in the corner, a sketch like Picture 2 appeared in the orange part. And it is impossible to delete it using remove redendant entities. how to solve it,, ?


Orange G is also a dye which is used in nucleic acid electrophoretic separation can other dyes like bromophenol blue can also be used? if not why? and which dye can be used in place of orange G.
I have attached below the literature for reference
Hi there,
I'm designing an experiment (not necessarily to be conducted) in which the primary source of nitrogen within large monocultures is manipulated, and yield of crop is measured over time. The objective is to determine the feasibility of using peanuts as a primary source of nitrogen on large-scale agricultural operations.
Site: large-scale (~200 ha) orange grove in Florida.
Treatments: one plot (1-ha) with only peanuts, one plot (1-ha) with some peanuts plus strategically applied synthetic fertilizer.
Control: one plot (1-ha) with no change in fertilizer regime.
Data will be collected from 5 subplots (400m2) per plot (15 total).
Recorded from each subplot will be number of trees and number of boxes of oranges yielded per tree. This data will be scaled up to estimate number of trees per hectare and number of boxes of oranges yielded per hectare.
Feasibility would be determined by change in yield over time. Little variation in average monthly, seasonal, and overall yield per plot would indicate potential feasibility of using peanuts as fertilizer. Great variation between experimental plots and control plot may indicate that peanuts are not suitable sources of nitrogen for monoculture oranges.
What would be the appropriate statistical test to analyze this data? I'm thinking a single-factor ANOVA will tell me differences between treatments, but how can I compare data per treatment over time to determine overall change? Repeated measures ANOVA?
Any suggestions/examples would be appreciated.
Thank you.
need to know the concentration calculation for my test
I am conduct my final project which is adsorption process of methyl orange using sugarcane bagasse as adsorbent. However, percentage of dye removal that I get was negative value because the final concentration that I get from adsorbance reading is higher than initial concentration. What should I do to get positive value of percentage dye removal ? Is it relate with the concentration of methyl orange or the amount adsorbent?
The figure shows the FTIR analysis for Plastic bag sample after three months of incubation with a fungal strain under special conditions. As demonstrated all T% at wave lengths in orange labels refer to standard FTIR polyolefins specifically LDPE as stated by Wiley Spectra Base (2020) in http://spectrabase.com/spectrum/IWxs9GBuNp. The other minor dips appearing in the carbonyl and double bond region blue labeled, and those in the finger-print region labeled in green were confusing, so please I need your help. FTIR of control (untreated Plastic bag) in red curve and that of treated in blue curve.

I need the detail of method such as the type of solvent and the wavelength . I want the steps in detail.
With special reference to India and its neighbouring country.
Sweet Orange, mandarin etc.
I would like some advise from those who have worked doing XRF for metamorphic rocks with large (centimetric-size) garnet samples. I have done XRF analysis to make pseudosections from these (these are meta-volcanic rocks (Amph, Opx,Plag,Grt,Bt,Ti-Fe oxides)), but I have obtained probably abnormal high Fe % values (~19%), and was advised to make again the bead for XRF. I was wondering what special procedure should I do to make powder from my sample. Do I need to make powder out of a large volume of sample? If so, what is specified as a 'large' volume of sample. Also, there is some yellow/orange alteration (oxides?) in some of the crushed sample, do I need to take away the yellow/orange alteration from it? I Would greatly appreciate any advise!
We are interested in evaluate the nitrate levels in leaves of sweet orange trees. Do you know a method to do it? We are particularly interested in one that demand few leaves.
Thank you
Luciana Marques de Carvalho
Methyl orange in its structure has a ion of Na+. my question is, after dissolving methyl orange in water, what will happen to that ion? Dose it form NaOH in reaction with water ? consequently, dose the H+ make a combination with methyl Orange? Or if not, do you know what will take place?
The solution of PGMs is include Pd,Pt and Ru ions, it is a solution with orange color. I has a problem for determination of chlorine ion in this solution by titration ( with AgNO3 ), the amount of chlorine ions is very low and due to color of solution, I cannot recognize final point of titration. please help me to found better method.
Recently I have followed a well accepted method to synthesize Cerium (4) alkoxide, The procedure is attached, I have used procedure number 4, but I obtained nothing but some orange small agglomerates stuck to the bottom of RB, Could you please help me with the possible reasons of this problem? and also is it important to follow the amount of precursors? I am thinking to multiply the amount of precursors (considering the stoichiometric relation ) with maintaining the amount of THF, otherwise in one round procedure the yield CeAlkoxide is only approximately 1 g, Does it lead to any problem?
I have coded an ML project stock price prediction, but unfortunately my predicted values follows the training data to some extent and then shows a straight line. Why is this so?
I have attached the code and the graph. Kindly guide me.
Orange line shows training data, green shows test data. Y-axis shows closing price and X-axis shows the date

After extraction of the essential oil, I found a high percentage of limonene presenting more than 90%.
Can I separate the pure limonene from the minority that presents less than 10%?
what would be the recommended protocol of acridine orange staining on hydrogel seeded cells by looking in Fluorescent microscopy. can someone have an idea about the concentration of a stock solution of AO, How much AO/PI to use, and what Quantity of cells to use for hydrogel?
We have used batch mode ultrasonic assisted adsorption experiments were conducted to study the methyl orange dye removal efficiency using some adsorbent. For each experiment, 100 mL of MO dye solution with prefixed concentration and pH were taken into a beaker and a suitable adsorbent dose was mixed with the solution. Thereafter the mixture solution was sonicated for a prefixed time by using an ultrasonic bath reactor (Rivotek, India) operated at an ultrasound frequency of 30 kHz. The ultrasonic bath reactor was having heater power of 130 W and ultrasound power of 50W, respectively. However the heater power was not used in this study as all the experiments were conducted at room temperature. The volume of water in the ultrasonic bath was 1.5 L.
Now we need to measure the intensity of ultrasound in the reaction vessel (i.e. in the beaker itself) during the reaction. Please provide the steps to measure the intensity of ultrasound in the beaker.
after dilute acid hydrolysis of orange peel should I neutralize the hydrolysate before or after dosage of sugar?
I am currently working on LAMP. Since the original mastermix volume was for 100 reactions and to prevent continuous freeze thaw of the mastermix we put the mastermix into smaller volumes and store at 20 degree celsius until we are using it. However, the mastermix in the tubes turned orange instead of remaining pink. Is there any reason why it is turning orange or is there any way to prevent it from turning orange.
Related to data mining/data science, I recently discovered Orange (https://orange.biolab.si/) a high level very powerful free toolkit. Using it is very simple and you can test different models in order to evaluate the best results. However, when writing paper to a Journal, is it plausible to use it instead of R Studio (for instance) or other tools (PyTorch, Weka, TensorFlow)? Or Orange is best indicated just for teaching?
Hi all, I conducted a binary log regression(yes/no) on 2 independent variables (categorical) called 'Color' and 'Income' using SPSS.
For 'Color':value1=blue , value2=orange , value3= green.
My reference category is Value3=green.
My question is how do I find out if a specific Color is sig or not since SPSS will always use one of the value as baseline and the results become a comparison of 'odds of value 2 vs odds of value 3 being a YES'. I will like to know if value2=Orange is significant in affecting the dependent variable result.
Is recoding each value in 'Color' into a binary variable (independent) then performing the test again correct?
This is a rough sketch of the SPSS output ' Variables in the equation' table:
Color
Color(1)
Color(2)
Kindly conduct the papers and mechanism and methods of Extraction of Bioactive Compounds from Orange Processing By-Products
So i have data something like this in SPSS at the moment:
case 1: orange, bananas, mangoes
case 2: bananas, oranges, mangoes
So the first respondent ranked orange as best fruit whilst second ranked banana as the best fruit.
- The variables i used were strings i.e. o, b and m, should this be a problem?
- Secondly, how do i assign rank to a particular column e.g. orange should be ranked 1 as it is in left most column, when the data was imported from sogosurvey ? See ranking.png for the actual data
- Also, is there a quick way to add replacement variable e.g. if the case has value "x" transform it to "yes" and if empty convert it to "no"? The empty cells mean not selected (=no OR 0) and M-multivariate course selected (=yes OR 1) in emptycells.png.
Thanks for your help.
How much raw materials are required for producing 1 kg of final products for each of the following?
1. Orange jam 2. Orange Jelly 3. Orange Marmalade 4. Tomato sauce 5. green Chilli Sauce 6. Tomato puree 7. Ginger paste 8. Canned pineapple
I am mixing sodium hydroxide with a stabilizing agent PVP and the solution turns a brown orange. Does that mean that a reaction is taking place? Is there a formation of some sort of gel? The pH is very basic (11-12).
Hi
I wondering about how to interpret results in model view (after double cliking) on results, when paireswise chart with orange color of pairs, and the table after with some values with orange too (Adj. sig)
I have a dependent variable called: Organizational loyalty
I have also categorical variable called: job category (1. execustion, 2 maitrise, 3 cadre, 4 cadre superieur, 5 Hors categorie) in english (1 execution, 2 mastery, 3 manager, 4 senior manager, 5 post category manager)
I hope I was clear in my question
Two new postings:
16. Can Orange County, CA Begin Opening this Week?
This was a high profile article in the Orange County Business Journal and explains that CA and OC have not (yet) been hit that hard, but still very vulnerable particularly as LA is now hitting epidemic proportions
17. Weekly Update: Kudos to NY and NJ
Discusses new updates with U.S. lagging the rest of the world, but at least NY and NJ make real progress toward recovery.
#covidresearch #covidmodeling #coronavirusupdate
We use 200 W tungsten lamp as source of light for degradation 20 mL methyl orange solution (10 ppm). How can calculate the irradiation intensity at the catalyst surface (mW/cm2)?
Does anyone have advice on how to quantify the cells of the anterior pituitary from normal PAS and Orange Staining? I am aware that this is outdated because we have immunohistochemistry, but I am searching to see if there are any publications with the use of Image J or any other software for counting cells.
My boss is using MMSE to test schizophrenia patients' cognitive function. However, the version has a total score of 39. He hopes me to find the origin of the version, but all I found is the version with a 30 maximum score.
The version we are using includes questions like: can you tell me the similarities between oranges and apples? can you tell me the differences between tables and chairs?
Has anyone seen or heard about this version? That would be super helpful!!!!!
Given a time series with Events (chart patterns), I want to assess whether events (chart patterns) occur systematic or randomly in a time series.
My idea is to
- identify patterns in a given time series
- simulate multiple new time series
- identify patterns in the simulated time series
- compare the results
The key question is how to compare the results. The events (chart patterns) have a specific length and can be overlapping (as illustrated in the image).
I could easily just use the number of events in the original time series and compare them to the count/number of events in the simulated time series by simulating enough time series to construct a confidence interval.
But this would not account for clustering, overlapping and the size of events. Hence, it is not enough to just compare the count/number of events (patterns) but also if there is clustering (margin between events) or differences in the size of the patterns.
I could do the same test for different statistics. But I am wondering if there is a more elegant way.
The image is an example with orange lines being events of different size/length in time. They are overlapping but do not seem to be clustered. If they would be clustered they might only appear in the beginning and end of the time series.
My question is:
How to test if events (with different size, overlapping, and maybe clustered) occur systematic in a time series or randomly?
Are there any similar problems in other disciplines?

I am working on research to apply six sigma in controlling the quality of the products of an industrial company, I did a case study
The supervisor asked me to use machine learning in the search (eg orange tool)
Do you have ideas or suggestions
Why are concrete and red-brick buildings cyan in infrared false color (IRFC) images?
Most building materials are made of soil (e.g. red tile roofs or concrete), thus most urban areas have the spectral signature of a soil.
Nevertheless, in IRFC satellite images we usually see bright urban areas in Bright Cyan instead of Dark Brown/LightBrown to Orange or Whitish. why is that?
I was looking for methods for the carotenoids analysis in the urchins' gonads, however, I found that all methods lacked important information such as the initial sample weight, the ratio of sample to solvent, the time for the sample to settle after adding solvents such as n-hexane or Petroleum Ether. Moreover, there is a wide variety of solvents or mixture of solvents used for the same species and each give different results. Also, as mentioned in Chandrika Liyana-Pathirana et al. (2002), after separation of the carotenoid extract in the hexane layer, the reference says to transfer the hexane to 50 ml volumetric flask and make up the volume with hexane, but my extract was already 65 ml, so when I calculate the total carotenoid with the equation, should I use 50 ml or 65 ml?
I have another question, when I have a treatment of triplicate, to measure carotenoid, should I mix samples from all triplicates or should I measure one individual from each replica and then calculate the mean?
My feeding experiment yielded many variation in the color of gonads within the same treatment. So should I discard the very odd color, for example the red, as it occurred in 2 individuals only who appeared diseased and loosing spines, while the remaining samples fluctuated between medium orange roe to dull orange?
I would much appreciate your help with the above specific details in order to be able to run an accurate analysis.
Thank you in advance.
its colorimetric detection after interacting a analye with nanoparticles color rapidly change from yellow to orange. after 15 min of interaction take of a absorption spectra only quenching (decrease in absorption intensity) was observed large nanoaggregates was formed which confrmed by DLS. after 45 min visible aggregates are seen in solution. why shift not appear ?
I am trying to prepare CuN(CN)2 (=Cu[dca] = Copper(I) dicyanamide) by reducing an aqueous Cu(II) solution to Cu(I) with K2[S2O5] then adding stoichiometric amounts of Na[dca]. However upon addition of the reducing agent I form an orange/brown precipitate in a green solution, I believe this is as a result of the disproportionation of newly formed Cu(I) to Cu (orange/brown precipitate) and Cu(II).
The overall reaction for the reduction is as follows:
Cu2+ + 2HSO3- + 2H2O --> Cu+ + 2HSO4- + 4H+
And the disproportionation:
2Cu+(aq) --> Cu(s) + Cu2+
Are there any other reducing agents which may work better or any ways to stabilise the Cu(I) formed in solution to prevent the disproportionation and allow further reaction with Na[dca]?
Hi everyone!
I need to study the interaction between a protein and a drug. However, the ligand binds to the hydrophobic regions of the protein, just as SYPRO Orange. Thus, I do not know if the compound and the dye will compete for the same binding sites. Is it possible to characterize the protein-ligand interaction in this conditions?
Thank you all in advance!
I use Qubit to quantify my DNA material, and I have never had quantification problems. However a few weeks ago my lab bought a new Qubit reactive which has a different color (slightly pink) in comparison to the first one (orange), and since that, we realized that there is a big difference in the quantification lectures between both kits.
I am performing in vitro anti-inflammatory assay: inhibiton of albumin denaturation by organic extract and purified fractions from natural material. Since my samples are colored (red, orange, yellow), I keep getting negative vaules, thus indicating pro-inflammatory rather than anti-inflammatory effect of extract/fractions. Concentration range of samples is 1 mg/ml to 62 ug/ml and the turbidity (absorbance at 660 nm) is higher with the increase of concentration. The results are the same even when I subtract the absorbance of blank (pure extract/fractions which almost do not absorb at wavelength mentioned above).
I am calculating the inhibition according to formula:
% Denaturation inhibition = (1−D/C)×100%
Where D is the absorbance reading of the test sample, and C is the absorbance reading without test sample (negative control).
I am measurng turbidity in 96-well plate; the solvent is methanol.
I was wondering if I am doing something wrong with my experiment setup or do you have any ideas how to avoid this problem?
According to the literature, there should be compounds in my extract/fractions that have already showed anti-inflammtory effects.
I used FITC Isomer I and ethanol and APTES under argon. The precipitate is bright orange. I want to use this for synthesis of nanoparticles (dye conjugated) and the precipitate will not help in this case.
I previously dispersed anhydrous FeCl3 in chloroform and turned gray to black and used it for thiophene polymerization. Now that I repeat the same method, the color of iron chloride in chloroform is orange and thiophene polymerization is not done either.
I am doing some simulations with the dyes dispersed in a PMMA and I want to test the device with different dye concentration (ppm). In order to do so, I need the molar absorptivity/molar extinction coefficient so that I can relate it to the transmittance and absorbance. I have the absorption and emission spectra of these Dyes, but I do not know their molar absorptivity/molar extinction coefficient, thus I can not compute the concentration (ppm) that I am using in the sample for the simulations. Does anyone know where can I found this data? I tried searching on the internet, but I did not find anything.
We use one probe with orange (Alexa555) dye to bind to the centromere of one chromosome and another probe with green (Alexa488) dye to bind to a gene. But in some nuclei we can see 5-10 spots that show both colours. However, in other nuclei in the same slide, there are only 2 orange and 2 green spot separately, as we expect it. What could cause this result?
Dear all,
I would like to know if the inner part of an immerged object is cleaned by an ultrasonic bath cleaner ?
In my schme, is the green part cleaned ? (orange and blue are two liquids.)

A while ago during a project I stumbled upon something interesting what happened when I left an aqueous solution of sodium salicylate (pH 14) in sunlight for an extended amount of time. The solution started the turn orange and eventually a dark red colour. I was curious to what this was so I decided replicate the conditions and try to isolate the formed product. The colour of the formed product seems to be dependent on the pH of the solution it is in, being red in basic solutions while yellow in acidic environments, the transition between the two seems to be around a pH of 7. The component itself also fluoresces in UV light. I found a articles describing observations on that the reaction took place under the influence of ultraviolet light, in a basic environment and that the reaction consumed oxygen from the air.
After isolating the component I was left with a off white powder, with possibly some NaCl (which can be seen in the spectrum later on) left in there from the neutralization steps and the removal of the salicylate (precipitating it out as salicylic acid by lowering the pH), most of that had been removed. A TLC, done a few days ago suggests that this is one single component and that no more salicylate is present in the sample. I took an FTIR spectrum of the sample, see the attached files.
While I can do interpretation, I am not that great at it. From what I can see here the arene is still there while both the hydroxyl and the carboxyl group are not present anymore. There does seem to be an aldehyde in the structure. (If my interpretation is correct, of course.)
Does anyone here know what reaction could be going on in this case?
Thanks in advance,
Dagomar Schreuder
I am trying to conduct a heterogeneous Fenton reagent using a natural iron oxide with Methyl orange as my contaminant of choice.
I have not seen any Methyl orange degradation even after 7 hours of reaction time. Does it matter when I add the hydrogen peroxide, should I rather let the solution equilibrate for an hour before adding the hydrogen peroxide. Should I rather use H2SO4 to adjust the pH?
When pbi2 is dissolved in dmf, it does not get 100% clear and there is some slight cloudiness. Also, when I create a one-step mapbi3 with these precursors, I'm getting orange cloudiness instead of clear yellow. Where is the problem? Do I have problems with precursors or solvent ?What can I do?
#perovskite #PbI2
I have tried several solvents such as DMF, THF, chloroform, and m-cresol. Out of these solvents, I think m-cresol mixed better with the polypyrrole powder where the solution changes from dark orange to black. Polypyrrole did not mix or dissolve with DMF, THF, and chloroform. The amount of polypyrrole mixed with m-cresol is actually very less where a considerable amount of polypyrrole powder left after filtration. For electrospinning, I use PU as a carrier for polypyrrole, but to no avail, I could not obtain polypyrrole electrospun fibers.
Thank you for your suggestion and opinion.
Hello! I am currently culturing SH-SY5Y cells and I have noticed change in the cells cultures over the last few weeks. Can someone please me identify what these are and if they are normal in SH-SY5Y cells?
Yellow and blue arrow image:
- Blue arrow: Is that an S-type cell? Is that normal to find in a SH-SY5Y cell culture?
- Yellow arrow: What is that?
Orange, Black, green arrow image:
- Orange arrow: little dots scattered all over the floor of the plate. What could it be?
- black arrow: finding a lot of circular dots. What are these?
- Green arrow: What is that? Is it normal?
Last question: Is it normal to have both S and N-type cells in a SH-SY5Y culture? Is there a way to separate the two?


I have an older bottle of formamide that has been maintained at 4C in amber glass, but which is contaminated with a denser orange liquid. This contaminant, while it's certainly liquid, doesn't seem to want to separate out into a discrete phase, but instead rolls around as a mass of individual droplets of ~1-3mm diameter. Does anybody have any ideas what this could be?
Thanks!