Science method

Chromatography - Science method

Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
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Publications related to Chromatography (10,000)
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Poster
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Oğul otu olarak da bilinen melisa (Melissa officinalis L.) bitkisinin düşük ve orta düzey sinir sistemi sorunlarının giderilmesine yardımcı olduğu bilinmektedir. Aktarlarda yaprakları kolay şekilde bulunabilmesine rağmen isim ve koku benzerliği sebebiyle bu tür bitkisel ürün satan yerlerde Melissa officinalis yerine halk arasında limon otu (Alloysi...
Article
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Biopharmaceuticals and their production are on the rise. They are needed to treat and to prevent multiple diseases. Therefore, an urgent need for process intensification in downstream processing (DSP) has been identified to produce biopharmaceuticals more efficiently. The DSP currently accounts for the majority of production costs of pharmaceutical...
Poster
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The manuscript titled "m R.N.A. PURIFICATIONS PROCESS : affinity chromatography , magnetic beads and graphene coated. Large scale Production and toxicological aspects" is very well written and has been accepted. Your article will get published soon as possible.
Article
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La purificación de enzimas celulasas constituye un paso crítico para su producción debido a que a menudo resulta ser muy costoso. El objetivo de este estudio fue evaluar la precipitación salina de celulasas producidas por Aspergillus niger UC33 en fermentación en estado sólido, para incrementar el rendimiento y pureza de la enzima con el menor cost...
Conference Paper
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Con il termine “polifosfati” viene indicata una categoria di additivi alimentari ammessi in diverse tipologie di prodotti di origine animale in quanto migliorano l’aspetto e la consistenza dei prodotti. La determinazione analitica di questi additivi è particolarmente complessa e molti autori hanno dimostrato che gli attuali metodi normati non sono...
Poster
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Il Panel sui rischi biologici (BIOHAZ) dell’EFSA, in una recente opinione scientifica ha sottolineato come l’identificazione di un prodotto di carne ottenuto da separazione meccanica (CSM) ha importanza sia dal punto di vista della qualità che della sicurezza alimentare. Lo stesso Panel ha sottolineato la necessità di dover identificare e classific...
Article
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Los Benzoxazinoides (Bx) son compuestos químicos derivados de ácidos hidroxámicos, con propiedadesaleloquímicas y antimicrobianas. En este estudio se reporta el efecto de Azopirillum brasilensesobre la producción de Bx en plántulas de trigo. El análisis por Cromatografía de Gasesacoplada a Espectrometría de Masas (CG/EM) de extractos de plántulas d...
Article
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Mariinskaya Gallery named after Maria Shapovalenko" fb.me/MariinskayaGallery, https://www.facebook.com/groups/435405866923911/media/albums, NGO "Information for all"), Russia, 121096, Moscow, POB 44. www.ifap.ru, , +7 (918) 443-00-43, +7 (988) 955-61-32, +7 (928) 434-20-84. papa888@list.ru, latyshev-1970@inbox.ru, olegl@ioso.ru, ima.
Preprint
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This study was planned to research the in vivo effects of lead (Pb) ions and sodium tetraborate (Na 2 B 4 O 7 ) on G6PD and 6PGD, which are some of the enzymes of the pentose phosphate pathway which carries vital importance for metabolism, and GR and GST, which are glutathione metabolism enzymes, and the in vitro effects of the same agents on the 6...
Preprint
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Simulated Moving Bed (SMB) chromatography is a well-known technique for the resolution of several high-value-added compounds. Parameters identification and model topology definition are arduous when one is dealing with complex systems such as a Simulated Moving Bed unit. Moreover, the large number of experiments necessary might be an expansive-long...
Article
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Bispecific antibodies (bsAbs), though possessing great therapeutic potential, are extremely challenging to obtain at high purity within a limited number of scalable downstream processing steps. Complementary to Protein A chromatography, polishing strategies play a critical role at removing the remaining high molecular weight (HMW) and low molecular...
Article
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Research on superheavy elements enables probing the limits of nuclear existence and provides a fertile ground to advance our understanding of the atom’s structure. However, experimental access to these atomic species is very challenging and often requires the development of new technologies and experimental techniques optimized for the study of a s...
Chapter
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Os impactos causados pelos microplásticos só podem ser efetivamente avaliados se as etapas de coleta, processamento e análises sejam realizadas de maneira apropriada. Hoje, a falta de normas, que deveriam delimitar os procedimentos metodológicos a serem seguidos, é, sem dúvida, uma grande limitação para o estudo desse contaminante. Apesar disso, um...
Article
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S-nitrosothiols (RSNOs) are a group of sulfur-containing compounds biologically involved in nitric oxide (NO˙) release and signalling pathway. NO˙ plays important physiological and pharmacological activities, particularly in vasodilation and in inducing muscle relaxation. Several RSNO compounds have been detected in biological systems, and many of...
Article
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In both linear and nonlinear chromatography, the lumped kinetic model is a suitable model for predicting elution bands when appropriate equilibrium functions and mass transfer coefficients are accessible. This model also works well in the case of gradient elution chromatography if variations in the equilibrium functions due to changes in the mobile...
Preprint
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In the present study, the mutual diffusivity D 11 in binary mixtures of water with technical polydisperse poly(ethylene) glycol (PEG) blends with molar masses of (1000, 4000, or 6000) g⋅mol ⁻¹ as well as with a purified monodisperse PEG homolog with a polymerization number of 21 and a molar mass of 943 g⋅mol ⁻¹ was investigated by heterodyne dynami...
Article
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Pollution by highly toxic contaminants poses a great threat to the aquatic environment and human life. Bimetallic materials have been proven to be efficient for the removal of such contaminants. In this study, the bimetallic Fe0/Ni0/alginate beads have been prepared using solvothermal technique followed by polymerization of alginate. Full-factorial...
Article
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This experiment is aimed at revealing the main characteristics of N2O emission from soybean in black soil and clarifying the relationship between N2O emission and environmental factors; in this experiment, static chamber-gas chromatography field observation method was used; meanwhile, three different drip irrigation amounts (300 mm, 350 mm, and 400...
Article
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The chromatographic column is the core of a high-performance liquid chromatography (HPLC) system, and must have excellent separation efficiency and selectivity. Therefore, functional modification materials for monolithic columns have been rapidly developed. This study is a systematic review of the recently reported functionalized monolithic columns...
Article
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Salah satu tanaman dari Kalimantan yaitu bawang dayak (Eleutherine bulbosa (Mill.) Urb.) secara empiris dapat digunakan untuk mengobati berbagai penyakit. Umbi bawang dayak diketahui mempunyai senyawa marker golongan naftokuinon yaitu elecanacin, eleutherin, eleutherol dan eleutherinon. Tujuan penelitian ini untuk menganalisis secara kualitatif gol...
Article
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Une substance à odeur de fraise/soufré est caractéristique de certains cépages non itis Vinifera. Cette substance sété mise en évidence par chromatographie en phase gazeuse , associéeà l'olfactométrie, à la photométrie de flamme et à la spectométrie de masse. Elle est formée par réaction chimique entre le furanéol (composé typique de cépages blancs...
Article
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A highly efficient technology for generating new monoclonal single-domain recombinant antibodies (nanobodies) was used to obtain a panel of nanobodies recognizing human apo- and/or holo-transferrin. This article is devoted to the primary analysis of the properties of two different variants of the new nanobodies obtained by us, as well as to the dem...
Book
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As nanomaterials’ unique properties can improve detection sensitivity and miniaturize the devices used in analytical procedures, they can substantially affect the evaluation and analysis ability of scientists and researchers and foster exciting developments in separation science. The book includes chapters on such crucial topics as the use of nanom...
Article
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Despite huge potential, automation of synthetic chemistry has only made incremental progress over the past few decades. We present an automatically executable chemical reaction database of 100 molecules representative of the range of reactions found in contemporary organic synthesis. These reactions include transition metal–catalyzed coupling react...
Article
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Espécies do gênero Ocimum, também conhecidas como manjericão e/ou alfavaca, são utilizadas para a prevenção de diversas enfermidades, como temperos culinários e aromatizantes. Neste estudo, três espécies deste gênero foram avaliadas quanto à composição química e o fator de proteção solar (FPS) dos óleos essenciais extraídos das folhas das espécies...
Chapter
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Algumas espécies pertencentes ao gênero Machaerium (Fabaceae) são de uso etnofarmacológico, como é o caso de Machaerium acutifolium Vogel. Esta planta é utilizada na medicina popular no tratamento de dor e inflamações. Muitas espécies de plantas medicinais, se empregadas e extraídas indiscriminadamente, podem ser levadas à extinção, assim como o re...
Article
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Introduction: Different types of Snehapaka have been mentioned in Sarangdhar Samhita such as Mrudu Paka, Madhyama Paka, Khara Paka, Dagdha Paka, Ama Paka, and Gandha Paka. Further, different uses of sneha according to its paka had been mentioned in which Madhyama Paka and Khara Paka are stated to be used for internal (Pana) and external (Abhyang),...
Article
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The characterization and modelling of membrane chromatography processes require the axial dispersion coefficient as a relevant and effective intrinsic property of porous media, instead of arbitrary assumptions on pore size distribution. The dispersion coefficient can be easily measured by experiments completely independent of chromatographic tests....
Conference Paper
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Dişli ve Karabağlar (Bolvadin) çevresinde kömür ve bitümlü şeyl varlıklarına yönelik olarak öncel çalışmalar vardır. Ayrıca, çalışma alanı yakınında detaylı etütleri yapılmamış bir kömür mostrası bulunmaktadır. Bu nedenle, bölgedeki kömür ve bitümlü şeyllerden türemesi muhtemel hidrokarbonların, uygun jeolojik koşullara sahip olan Emirdağ’daki form...
Article
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A relação entre medidas restritivas de mobilidade urbana devido à pandemia por COVID-19 e a poluição do ar atmosférico resultou em estudos sobre o impacto das medidas de proteção contra o avanço da doença na qualidade do ar. Neste contexto, amostras de material particulado fino foram obtidas em período de restrição parcial e relaxamento de mobilida...
Conference Paper
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Los derrames de petróleo representan una problemática para las autoridades responsables de regular este tipo de siniestros ambientales. Esto se debe a que una vez que los hidrocarburos han impactado sobre sistemas acuáticos comienzan a actuar sobre ellos diferentes procesos de meteorización que modifican su composición. Como resultado se dificulta...
Article
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Sulfonamides are a classic group of chemotherapeutic drugs with a broad spectrum of pharmacological action, including anticancer activity. In this work, reversed-phase high-performance liquid chromatography and biomimetic chromatography were applied to characterize the lipophilicity of sulfonamide derivatives with proven anticancer activities again...
Preprint
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Asparaginyl endopeptidase is a useful biocatalyst for site-specific protein bioconjugation. However, existing recombinant protocol is lengthy requiring cap removal of the zymogenic enzymes through acid activation and extra chromatographic steps. Here, we describe an activation-free approach for AEP preparation where the cap and core domains are pre...
Article
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The convective transport rate of polymers through confined geometries depends on their size, allowing for size-based separation of polymer mixtures (chromatography). Here, we investigate whether mixtures of active polymers can be separated in a similar manner based on their activity. We use thin, living Tubifex tubifex worms as a model system for a...
Technical Report
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Goals • To apply modern, automated electrolytic eluent generation to the separation of carboxylic acids by ion-exclusion chromatography to increase the ease of use • To develop an inexpensive and environmentally friendly external chemical regeneration for suppressed conductivity detection in high-performance ion-exclusion chromatography
Poster
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The microorganisms responsible for the biotransformation of expanded perlite into organic-like substrates were analyzed for compatibility, interspecific synergy (bacteria, fungi, diazotrophs), the organic content (by chromatography), humic-like fractions, siderophores, enzyme complex, seed germination and the growing of plantlets. The results refle...
Article
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Rheological analysis using multiple frequencies and different temperatures and X-ray analysis were performed on previously synthesized paramylon acetate laurates and paramylon acetate myristates with three different degrees of substitution [DSs; 0.56 or 0.50 (high), 0.39 or 0.35 (moderate), 0.20 or 0.19 (low)] of long-chain acyl groups to gain insi...
Article
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Centrifugal partition chromatography (CPC) and countercurrent chromatography (CCC) are two preparative techniques mainly used for the isolation and purification of natural products. While CPC benefits from a larger sample capacity, CCC typically provides better peak resolutions and hereby higher purities. In this study, we aimed to combine both adv...
Article
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Traditional Chinese medicines (TCMs) have been widely used in clinical and healthcare applications around the world. The characterization of the phytochemical components in TCMs is very important for studying the therapeutic mechanism of TCMs. In the analysis process, sample preparation and instrument analysis are key steps to improve analysis perf...
Article
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Metal- or acid-catalyzed intramolecular hydroamination and Cope-type intramolecular hydroamination, a distinct concerted approach using hydroxylamines, typically suffer from significant synthetic limitations. Herein we report a process for intramolecular hydroamination that uses a redox-enabled strategy relying on efficient in situ generation of hy...
Article
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In order to improve the weak optical performance of gold nanoparticles and realize the signal amplification of lateral flow chromatography test strips, individual gold nanoparticles (AuNPs) were aggregated into gold nanoparticle aggregates through functional groups around polyamidoamine (PAMAM) dendrimers. A signal-amplified aptamer-based lateral f...
Article
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Although the Republic of Croatia is a relatively small country geographically, it can boast numerous scientists who have left indelible marks in various scientific fields. However, this paper is exclusively about chromatography as one of the most important analytical techniques of our time. The development of chromatography in the Republic of Croat...
Article
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Darbeli Elektromanyetik Alan (PEMF) elektromanyetik alanların iyonlaştırıcı olmayan formlarından biri olup nörodejeneratif bozuklukların semptomlarının tedavisi gibi çeşitli tıbbi problemler için alternatif bir tedavi olarak kullanılmaktadır. Çalışmanın amacı, yüksek doz hidrojen peroksit (H2O2) ile muamele edilen insan nöroblastoma hücre hattında...
Article
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Na perspectiva de uma inclusão educacional no contexto de educação especial é discutido o papel da escola no acesso ao conhecimento dos alunos com deficiência e como é contemplado esse processo de ensino-aprendizagem. Nesse sentido, o objetivo desta pesquisa é desenvolver uma flexibilização em prática experimental de ensino de ciências para alunos...
Article
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Chromatography is a technique widely used in the purification of biopharmaceuticals, and generally consists of several chromatographic steps. In this work, Hydrophobic Interaction Chromatography (HIC) is investigated as a polishing step for the purification of therapeutic proteins. Adsorption mechanisms in hydrophobic interaction chromatography are...
Article
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In order to use mechanistic models in chromatography, model parameters must be exact. Mechanistic models have a difficult time simulating the harvest of cleared cells. Another major roadblock is a lack of specimen complex and time to establish all of the desired criteria. This research was done in order to develop a process that starts with complex...
Article
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An extended method of semidiscrete high-resolution finite volume is used in this paper to obtain numerical solutions for a formulated nonlinear lumped kinetic model of liquid chromatographic process to examine the effect of chromatographic column overloading gradient elution considering core-shell particles. The model constitutes linear solvent str...
Presentation
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Chemical studies are a hot and challenging topic in the superheavy element (SHE) research field. The drastically decreasing production cross sections and half-lives with increasing proton number result in a small number of detected events. The chemical study of volatile superheavy elements Cn (Z = 112) and Fl (Z = 114), which have closed electron-s...
Book
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RESUMO Tendo em vista os vários problemas ambientais e de saúde que o uso crescente de agrotóxicos vem causando, é necessário a otimização de técnicas que visem a sua rápida degradação. A atrazina é um herbicida utilizado no controle de ervas daninhas, principalmente nas culturas de milho e cana-de-açúcar. Nesse sentido, o objetivo deste estudo foi...
Article
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RESUMO Tendo em vista os vários problemas ambientais e de saúde que o uso crescente de agrotóxicos vem causando, é necessário a otimização de técnicas que visem a sua rápida degradação. A atrazina é um herbicida utilizado no controle de ervas daninhas, principalmente nas culturas de milho e cana-de-açúcar. Nesse sentido, o objetivo deste estudo foi...
Article
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The development of chromatographic science in Ukraine is due to the widespread use of chromatography to solve practical problems. The centers of chromatographic research in Ukraine are in large cities: Kyiv, Kharkiv, Lviv, Odessa, and others. Along with the development of chromatographic research methods, analysis methods for control of food and ag...
Article
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We propose a new ‘active particle’ system in which the particles are in fact polymer-like. We experimentally study the rheology of long, slender, and entangled living worms (Tubifex Tubifex, or ‘sludge worms’). Performing classical rheology experiments on this entangled polymer-like system, we find that the rheology is qualitatively similar to that...
Research
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The distribution of synthetic pyrethroids in the surface waters was assessed by measuring the concentration in water samples. For this purpose water was collected agricultural field CCS HAU, Hisar. Water samples (200 mL) were treat cyhalothrin extraction of the pyrethroid from the water. chromatography equipped 90 days with half (0.001 Copyright ©...
Article
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Knowledge of the chemical-physical reactions that determine the main degradation behaviour of artists' alkyd paints represents one of the main problems within the museum exhibitions. The collection and interpretation of these data on degradation phenomena, especially after ozone exposure at different relative humidity values, can be useful for thei...
Article
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Large-scale target cell isolation from patient blood preparations is one of the critical operations during drug product manufacturing for personalized cell therapy in immuno-oncology. Use of high-affinity murine antibody coated magnetic nanoparticles that remain on isolated cells is the current standard applied for this purpose. Here, we present th...
Article
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Traditional column liquid chromatography in the overwhelming majority of cases is based on the use of equilibrium adsorption and desorption processes in flow systems. In this case, the selectivity of separation of compounds is determined primarily by the combination of chemical properties of the sorbent, eluent and sorbate or, in other words, by th...
Poster
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Forensic identification of human use compounds in municipal storm water by solid phase extraction liquid chromatography tandem mass spectrometry
Article
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Research on the effect of storage time on blood quality has been conducted. The purpose of this study is to determine the quality changes caused by the length of storage. The test parameter is the change in blood to become HIV positive. The blood testing process is carried out by immune chromatography on blood that has been stored for 2 weeks, 3 we...
Article
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The determination of the concentrations of the organic compounds in ambient air using reference methods is a time-consuming process. The samples must be collected on a specific sorbent material and analyzed. In the best-case scenario, the analysis may be performed on site by portable chromatography techniques; otherwise, transport and laboratory an...
Article
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Process chromatography modelling for process development, design, and optimization as well as process control has been under development for decades. Still, the discussion of scientific potential and industrial applications needs is open to innovation. The discussion of next-generation modelling approaches starting from Langmuirian to steric mass a...
Article
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En el petróleo existe un grupo de moléculas conocidas como biomarcadores capaces de permanecer estables durante las agresivas condiciones en las que aquel se forma. En el presente trabajo se analizó la estabilidad ambiental de los biomarcadores de un crudo sometido a intemperización artificial en agua de mar y suelo, con el objetivo de probar su ut...