Questions related to Chemical Neuroanatomy
I do stereotaxic injections into mPFC of mice brains. I've been trying to figure out a way to overlay a mouse brain atlas outline onto images of brain slices in order to determine the accuracy and precision of injections and if our expression pattern is within our target area.
I think there's a way to use ImageJ to do it, but I haven't had any luck as of yet.
I was wondering what programs and methods others have used and if they could provide either a brief walk through or provide a link to a paper or source that describes the process.
The image I've attached is a great example of what my end result should look like. I just don't know how to get there.
Thanks in advance.
Does anyone have a method to determine the difference by morphology alone i.e. without the use of an additional label such as synapsin for terminals or tau for axons? Our neurons are filled with virally-expressed eYFP.
Hi, I am trying to mark (immunohistochemically) blood vessels of bird brains. I have slices (thickness 50um) of the tissue and, to mark, I am trying to use antibodies that work in the basement membrane. I have tried anti-laminin (sigma) and anti-collagen IV (polyclonal, abcam), but none of these has worked (sometimes it marks only in some regions) in the bird tissue. Someone has already tried to mark brain vasculature in birds? Someone with any suggestions? I'll be very grateful with any help.
PS. I am doing an imunofluorescence work - as I'll get the images on the imuno-fluorescent apotome microscopy.