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Background Neuroblastoma is a deadly childhood cancer, and MYCN -amplified neuroblastoma (MNA-NB) patients have the worst prognoses and are therapy-resistant. While retinoic acid (RA) is beneficial for some neuroblastoma patients, the cause of RA resistance is unknown. Thus, there remains a need for new therapies to treat neuroblastoma. Here we exp...
Article
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Dengue is a tropical disease caused by the Dengue virus (DENV), a positive-sense, single stranded RNA virus of the family Flaviviridae, which is transmitted by Aedes mosquitoes. The occurrence of dengue has grown dramatically around the globe in recent decades, and it is rapidly becoming a global burden. Furthermore, all four DENV serotypes cocircu...
Article
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Oxysterol-binding protein (OSBP)-related protein (ORP) 6, a member of subfamily III in the ORP family, localizes to membrane contact sites between the endoplasmic reticulum (ER) and other organelles and functions in non-vesicular exchange of lipids including phosphatidylinositol-4-phosphate (PI4P) in neurons. In this study, we searched for the lipi...
Article
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Doxorubicin (Dox) is a highly effective chemotherapeutic agent employed in the handling of hematological and solid tumors. The effective use of Dox in cancer therapy has been seriously limited due to its well-known cardiotoxic side effects, mainly mediated by oxidative damage. Therefore, the identification of an effective and safe antagonist agains...
Preprint
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Life is a nonequilibrium phenomenon: metabolism provides a continuous supply of energy that drives nearly all cellular processes. However, very little is known about how much energy different cellular processes use, i.e. their energetic costs. The most direct experimental measurements of these costs involve modulating the activity of cellular proce...
Article
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Cell techniques undergo rapid advancement across different areas of biomedical research [...]
Chapter
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In this chapter, we compare the development of two brown algae, the tiny filamentous brown alga Ectocarpus sp. and the large parenchymatous brown alga Saccharina latissima. Owing to their major morphological and life cycle differences, broadly described in this chapter, these algae are now two useful models for comparative studies on embryogenesis,...
Article
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Background Glioblastoma (GBM; grade IV glioma) is characterized by a very short overall survival time and extremely low 5-year survival rates. We intend to promote experimental and clinical research on rationale and scientifically driven drug repurposing. This may represent a safe and often inexpensive way to propose novel pharmacological approache...
Article
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Surface layers (S-layers) are proteinaceous crystalline coats that constitute the outermost component of most prokaryotic cell envelopes. In this study, we have investigated the role of metal ions in the formation of the Caulobacter crescentus S-layer using high-resolution structural and cell biology techniques, as well as molecular simulations. Ut...
Research Proposal
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ANR) for up to 30 months /Team UrGenT, IRSET-Inserm U1085, Rennes, France. OFFER: We offer a postdoctoral position in a multidisciplinary environment that will provide opportunities for impactful research. As part of an ANR funded-research project, the recruited candidate will develop researches on the mechanisms responsible for the establishment o...
Preprint
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Background Glioblastoma (GBM; grade IV glioma) is characterized by a very short overall survival time and extremely low 5-year survival rates. We intend to promote experimental and clinical research on rationale and scientifically driven drug repurposing. This may represent a safe and often inexpensive way to propose novel pharmacological approache...
Article
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Hydroxytyrosol (HT), the major phenolic compound in olive oil, is attracting increasing interest for its beneficial properties including a notable antioxidant and anti-inflammatory power. In this study, using a combination of biophysical and cell biology techniques, we have tested the role of HT in the formation of advanced glycation end-products (...
Conference Paper
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RATIONALE: Mesothelial to mesenchymal transition (MesoMT) is one of the crucial mechanisms underlying pleural fibrosis, which can result in restrictive lung disease. DOCK2 plays important roles in immune functions and inflammatory processes, however, whether it plays a role in pleural fibrosis particularly MesoMT remains unknown. METHODS: Immunohi...
Article
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Adenoviruses contain dsDNA covalently linked to a terminal protein (TP) at the 5′end. TP plays a pivotal role in replication and long-lasting infectivity. TP has been reported to contain a nuclear localisation signal (NLS) that facilitates its import into the nucleus. We studied the potential NLS motifs within TP using molecular and cellular biolog...
Article
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Deterioration of insulin secretion and pancreatic beta-cell mass by inflammatory attacks is one of the main pathophysiological features of type 2 diabetes (T2D). Therefore, preserving beta-cell mass and stimulating insulin secretion only in response to glucose for avoiding the hypoglycemia risks, are the most state-of-the-art option for the treatme...
Thesis
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Mitochondria are organelles in eukaryotic cells, which through oxidative phosphorylation (OXPHOS) produces most of the ATP used to drive cellular processes. The organelle contains its own genetic material, mitochondrial DNA (mtDNA), which encodes 13 key components of the OXPHOS machinery. For its maintenance and expression, mtDNA is dependent on a...
Conference Paper
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The loss of b-cell function and b-cell death is key feature of type 2 diabetes (T2M). Different hypothesis is presented to contribute to this disease including amyloid formation by the human islet amyloid polypeptide (hIAPP). Despite the prevalence of T2M on the world, there are no therapeutic strategies for the treatment or prevention of amylin am...
Article
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Beta‐cell death is the key feature of type 2 diabetes mellitus (T2DM). One hypothesis for the mechanism of this feature is amyloid formation by the human Islet Amyloid Polypeptide (hIAPP). Clinical trials and population studies indicate the healthy virtues of the Mediterranean diet, especially the extra virgin olive oil (EVOO) found in this diet. T...
Article
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Despite the availability of antibiotics and vaccines, many intractable infectious diseases still threaten human health across the globe. Uncontrolled infections can lead to systemic inflammatory response syndrome and the excessive production of inflammatory cytokines, known as a cytokine storm. As cytokines also play necessary and positive roles in...
Article
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Negeviruses (NVs) are a recently discovered taxon of enveloped, positive sense, single-stranded RNA viruses, infecting blood-sucking insects. While classical arthropod-borne (arbo)viruses like dengue and Chikungunya infect both insects and vertebrates, NVs are restricted to insects and do not have any known vertebrate host and are thus classified a...
Article
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Hydroxytyrosol (HT), one of the main phenolic components of olive oil, has attracted considerable interest for its biological properties, including a remarkable antioxidant and anti-inflammatory power and, recently, for its ability to interfere with the amyloid aggregation underlying several human diseases. We report here a broad biophysical approa...
Preprint
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When diagnosed early, people with breast cancer often have a very good prognosis. But when cancer cells begin to metastasize, treatment becomes more difficult. Metastasis begins with the formation of a pre-metastatic niche (PMN), which has favorable conditions to direct the growth and migration of tumor cells. Inhibiting this process can prevent ca...
Article
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Loss of β-cell function and β-cell death is the key feature of type 2 diabetes mellitus (T2DM). One hypothesis for the mechanism of this feature is amyloid formation by the human islet amyloid polypeptide (hIAPP). Despite the global prevalence of T2DM, there are no therapeutic strategies for the treatment of or prevention of amylin amyloidosis. Cli...
Article
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Over the course of three months (December 2019-March 2020) the pandemic of the Coronavirus Disease 2019 (COVID-19) (1), caused by a zoonotic virus (2), the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), has raised multiple concerns and triggered an unprecedented and deep impact across multiple areas of biomedical research, especially...
Article
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Background: Formation and maintenance of appropriate neural networks require tight regulation of neural stem cell proliferation, differentiation, and neurogenesis. microRNAs (miRNAs) play an important role in brain development and plasticity, and dysregulated miRNA profiles have been linked to neurodevelopmental disorders including autism, schizop...
Article
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Background & aims: Functional interactions between MOR and DOR represent a potential target for novel analgesics and may drive the effects of the clinically-approved drug Eluxadoline for the treatment of diarrhea-predominant irritable bowel syndrome (IBS-D). Although the enteric nervous system (ENS) is a likely site of action, the co-expression an...
Technical Report
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The term OMICS refers to scientific disciplines that study different types of biological molecules that make up complete biological systems. The suffix “-omics” comes from the Greek word “-ome,” which means “whole.” Recent advances in laboratory methods, known as high-throughput techniques, allow for automation and thus increased efficiency in clas...
Chapter
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Dark microglia, a recently described phenotype, are found in high numbers in nonhomeostatic conditions (e.g., Alzheimer’s disease pathology, aging, chronic stress). As a specific protein marker has not yet been defined, they cannot be studied using conventional cellular biology techniques. They are recognized by their unique ultrastructural feature...
Article
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Flow cytometry is a popular cell biology technique that utilizes laser-based technology to count, sort, and profile cells in a heterogeneous fluid mixture. Using a flow cytometer machine, cells or other particles suspended in a liquid stream are passed through a laser light beam in single file fashion, and interaction with the light is measured by...
Article
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Recent findings call for the critical overview of some incorrectly used plant cell and tissue culture terminology such as dedifferentiation, callus, totipotency, and somatic embryogenesis. Plant cell and tissue culture methods are efficient means to preserve and propagate genotypes with superior germplasm as well as to increase genetic variability...
Article
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About 20% of prostate cancer (PCa) patients progress to metastatic disease. Metabolic syndrome (MeS) is a pathophysiological disorder that increases PCa risk and aggressiveness. C-terminal binding protein (CTBP1) is a transcriptional corepressor that is activated by high-fat diet (HFD). Previously, our group established a MeS/PCa mice model that id...
Thesis
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Primary Ciliary Dyskinesia (PCD) is one of the ‘ciliopathies’, genetic disorders affecting either cilia structure or function. PCD is a rare recessive disease caused by defective motile cilia. Affected individuals manifest with neonatal respiratory distress, chronic wet cough, upper respiratory tract problems, progressive lung disease resulting in...
Conference Paper
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Background: Cytoplasmic tail (CT) of HIV-1 Env contains several motifs that are highly conserved across different HIV-1 clades and play critical roles in modulating multiple functions. One of the widely accepted approaches to produce soluble Env form or to enhance Env expression at cell surface is to truncate the CT. Here, we have studied naturally...
Article
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Dimethyl sulfoxide (DMSO) is a small molecule with polar, aprotic and amphiphilic properties. It serves as a solvent for many polar and nonpolar molecules and continues to be one of the most used solvents (vehicle) in medical applications and scientific research. To better understand the cellular effects of DMSO within the concentration range commo...
Article
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High-fat diet is associated with hypercholesterolemia and seminal alterations in White New Zealand rabbits. We have previously reported disorders in the development of the manchette-acrosome complex during spermiogenesis and decreased testicular efficiency in hypercholesterolemic rabbits. On the other hand, olive oil incorporated into the diet impr...
Conference Paper
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Introdução: O ácido hialurônico (AH) atua nas fases inflamatória, proliferativa e remodeladora da cicatrização(1), enquanto fatores de crescimento de fibroblasto (FCF) agem na regeneração cutânea(2). Receptores ativados de FCF fosforilam resíduos de tirosina que mediam vias de sinalização intracelular, tais como PI3K-AKT, e resultados ex vivo suger...
Poster
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Introdução: O ácido hialurônico (AH) atua nas fases inflamatória, proliferativa e remodeladora da cicatrização(1), enquanto fatores de crescimento de fibroblasto (FCF) agem na regeneração cutânea(2). Receptores ativados de FCF fosforilam resíduos de tirosina que mediam vias de sinalização intracelular, tais como PI3K-AKT, e resultados ex vivo suger...
Article
Full-text available
O ácido hialurônico (AH) atua nas fases inflamatória, proliferativa e remodeladora da cicatrização, enquanto fatores de crescimento de fibroblasto (FGF) agem na regeneração cutânea. Receptores ativados de FGF fosforilam resíduos de tirosina que mediam vias de sinalização intracelular, tais como PI3K-AKT, e resultados ex vivo sugerem sua relevância...
Article
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Current anti-cancer strategy takes advantage of tumour specific abnormalities in DNA damage response to radio- or chemo-therapy. Inhibition of the ATR/Chk1 pathway has been shown to be synthetically lethal in cells with high levels of oncogene-induced replication stress and in p53- or ATM- deficient cells. In the presented study, we aimed to elucid...
Conference Paper
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Introduction Hypoxia (low oxygen) is associated with worst prognosis and therapy resistance in most solid tumours. Hypoxia occurs in ~50% of breast cancer, most frequently in triple negative breast cancer (TNBC), the most aggressive subtype. Hypoxia drives tumour aggressiveness through HIF, which activates genes that promote adaptation to hypoxia,...
Book
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This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology...
Chapter
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Immunolocalization for transmission electron microscopy is a powerful technique to identify subcellular localization of proteins. This can be combined with molecular and physiological data in order to have a complete overview of protein function. However, optimal sample preservation is required to avoid artefacts. When using chemically fixed sample...
Article
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Induced pluripotent stem cells (iPSCs) offer the potential to generate tissues with ethnic diversity enabling toxicity testing on selected populations. Recently, it has been reported that endothelial progenitor cells (EPCs) derived from umbilical cord blood (CB) or adult peripheral blood (PB) afford a practical and efficient cellular substrate for...
Article
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Cellular processes are complex and result from the interplay between multiple cell types and their environment. Existing cell biology techniques often do not allow for accurate interpretation of this interplay. Using a quantitative imaging-based approach, we present a high-content protocol for characterizing the dynamic phenotypic responses (i.e. m...
Chapter
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The E. coli inducible lysine decarboxylase LdcI is an important acid stress response enzyme, whereas the AAA+ ATPase RavA is involved in multiple stress response pathways. A complex between these two proteins is thought to counteract acid stress under starvation in E. coli. We solved the crystal structure of the RavA monomer and combined it with a...
Article
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This paper describes the implementation of the Scientific Literacy in Cell Biology (SLCB) curriculum in an undergraduate biology laboratory course. The SLCB curriculum incorporated the reading and discussion of primary literature into hands-on and collaborative practical experiences. It was implemented in five stages over an 11-week period, during...
Book
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The completion of the human and other organisms’ genome sequencing project is an important development in the history of biological sciences in general and cytogenetics in particular. It happened because of many techniques that are developed time to time. Learning these techniques will permit students to cope up with the basic principles and will p...
Article
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Oxidative stress and reactive oxygen species (ROS) are common to many fundamental responses of plants. Enormous and ever-growing interest has focused on this research area, leading to an extensive literature that documents the tremendous progress made in recent years. As in other areas of plant biology, advances have been greatly facilitated by dev...
Presentation
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A pesar de que las personas de 80 años y más constituyen la gran mayoría con la enfermedad de Alzheimer (EA), son las menos representadas en los ensayos clínicos. Se han dado diferentes argumentos. Sin embargo, no se ha prestado suficiente atención a la posible interacción entre los procesos celulares y moleculares subyacentes al envejecimiento y l...
Article
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Cryo-electron microscopy (cryo-EM) is a structural molecular and cellular biology technique that has experienced major advances in recent years. Technological developments in image recording as well as in processing software make it possible to obtain three-dimensional reconstructions of macromolecular assemblies at near-atomic resolution that were...
Article
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This study established a goat embryonic kidney (GEK) cell line which was susceptible to influenza virus. Kidney cells were separated from fetus of Matou goat and GEK cell line was established by cell biology techniques. Tumorigenicity of GEK cell line was tested by tumorigenesis assay, and its marker protein expression was tested by immunohistochem...
Article
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Background: The incidence of cutaneous malignant melanoma continues to rise, and once the disease metastasizes it is almost inevitably fatal. We recently reported that a large miRNAs cluster on human chromosome 14q32, implicated in many types of cancers, is significantly down-regulated in melanoma. miR-377, one of the miRNAs located within this cl...
Article
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Peroxisomal biogenesis and function critically depends on the import of cytosolic proteins carrying a PTS1 sequence into this organelle upon interaction with the peroxin Pex5p. Recent structural studies have provided important insights into the molecular recognition of cargo proteins by Pex5p. Peroxisomal import is a key feature in the pathogenesis...
Article
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Background and purpose: The non-nucleoside analogue reverse transcriptase inhibitor efavirenz is associated with hepatic toxicity and metabolic disturbances. Although the mechanisms involved are not clear, recent evidence has pinpointed a specific mitochondrial action of efavirenz accompanied by the induction of an endoplasmic reticulum (ER) stres...
Article
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Invasive breast carcinomas are a group of malignant epithelial tumors characterized by the invasion of adjacent tissues and propensity to metastasize. The interplay of signals between cancer cells and their microenvironment exerts a powerful influence on breast cancer growth and biological behavior(1). However, most of these signals from the extrac...
Article
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RESUMEN Introducción: Los materiales de uso odon-tológico son sometidos a diferentes pruebas para determinar su compatibilidad, bioac-tividad, y demostrar que son aptos para permanecer en el medio oral sin producir una respuesta adversa. Con tal propósito ac-tualmente en el mundo se emplean distintas técnicas como los cultivos celulares, técni-ca...
Article
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To assess the utility of the desmopressin (DDAVP) test in the diagnosis and follow-up of a cyclical Cushing's disease (CCS) case. Laboratory tests included morning and midnight serum cortisol levels, 24h urine free cortisol excretion, midnight salivary cortisol levels, serum cortisol levels after low (1mg) and high (8mg) dexamethasone, plasma ACTH...
Article
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A review of therapeutic effects in preclinical and clinical studies suggests that concordance between large animal (pig=78%), small laboratory animal (53%) and in vitro (57%) results with those observed in humans is only partial. Pig models of wound healing provide major advantages over other animal models. Since the vast majority of wound-healing...
Article
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Significance: A review of therapeutic effects in preclinical and clinical studies suggests that concordance between large animal (pig = 78%), small laboratory animal (53%) and in vitro (57%) results with those observed in humans is only partial. Pig models of wound healing provide major advantages over other animal models. Since the vast majority o...
Article
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Background In dicotyledonous plant, the first asymmetric zygotic division and subsequent several cell divisions are crucial for proembryo pattern formation and later embryo development. Arabinogalactan proteins (AGPs) are a family of extensively glycosylated cell surface proteins that are thought to have important roles in various aspects of plant...
Chapter
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Adult mammalian brain preserves neural stem cells (NSCs) that contribute to postnatal neurogenesis. The last decade has seen a tremendous progress in the identi fi cation and analysis of NSCs in the mouse brain. A handful of molecular and cell biology techniques have been applied to con fi rm the existence and to address the functions of NSCs in th...
Article
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The functional annotation of genomes, construction of molecular networks and novel drug target identification, are important challenges that need to be addressed as a matter of great urgency1-4. Multiple complementary 'omics' approaches have provided clues as to the genetic risk factors and pathogenic mechanisms underlying numerous neurodegenerativ...