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Cell Line - Science topic

Established cell cultures that have the potential to propagate indefinitely.
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Publications related to Cell Line (10,000)
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A recent article pointed out COVID vaccines are ordinary means. I note two things that make this argument even stronger. First, a better source for one point. Second, the Church has avoided ever saying that merely testing on fetal cell lines is a reason Catholics might avoid that medical product.
Preprint
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Although population-scale databases have expanded to millions of protein-coding variants, insight into variant mechanisms has not kept pace. We present PROD-ATAC, a high-throughput method for discovering the effects of protein-coding variants on chromatin. A pooled library of variants is expressed in a disease-agnostic cell line, and single-cell AT...
Article
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Novel amino-substituted pyridoquinazolinone derivatives have been designed and synthesized as potential c-MYC G-quadruplex (G4) ligands, employing an efficient methodology. All the new compounds exhibited moderate to good antiproliferative activity against the human osteosarcoma U2OS cell line. NMR and docking experiments revealed that the recently...
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This manuscript explores the safety and potential efficacy of glucosodiene, an isomeric alkaline glucose compound, as a therapeutic agent for cancer treatment. The study focuses on conducting safety tests using BJ1 cells (Normal Skin Fibroblasts) and evaluates the cytotoxic effects of glucosodiene on these cells. The results indicate that glucosodi...
Article
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Eight rifamycin-related polyketides were isolated from the culture broth of a marine-derived bacterium Salinispora arenicola, including five known (2–5 and 8) and three new derivatives (1, 6, and 7). The structures of the new compounds were determined by means of spectroscopic methods (HRESIMS and 1D, 2D NMR) and a comparison of their experimental...
Article
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The mechanisms of cell death due to electroporation are still not well understood. Recent studies suggest that cell death due to electroporation is not an immediate all-or-nothing response but rather a dynamic process that occurs over a prolonged period of time. To investigate whether the dynamics of cell death depends on the pulse parameters or ce...
Preprint
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Identifying non-coding regulatory variants in the human genome remains a challenging task in genomics. Recently we advanced our leading regulatory variant database, RegulomeDB, to its second version. Building upon this comprehensive database, we developed a novel machine-learning architecture with stacked generalization, TLand, which utilizes Regul...
Article
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Certified RNA reference materials are indispensable for assessing the reliability of RNA sequencing to detect intrinsically small biological differences in clinical settings, such as molecular subtyping of diseases. As part of the Quartet Project for quality control and data integration of multi-omics profiling, we established four RNA reference ma...
Poster
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Applications of polymer nanoparticles in delivery of genes to an inner ear cell line.
Poster
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Iraqi Journal of Science College of Science – University of Baghdad Certificate of Reviewing a Scientific Article Awarded To Prof. Dr. Saif Jabbar Yasir AL-Mayah For Reviewing the Manuscript Entitled: The anti-cancer impact of genetically engineered Newcastle disease virus expressing GFP gene against U87-MG cell line
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Iraqi Journal of Science College of Science – University of Baghdad Certificate of Reviewing a Scientific Article Awarded To Prof. Dr. Saif Jabbar Yasir AL-Mayah For Reviewing the Manuscript Entitled: The anti-cancer impact of genetically engineered Newcastle disease virus expressing GFP gene against U87-MG cell line We Highly Appreciate your Va...
Preprint
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To fulfill the potential of small interference RNA (siRNA) therapeutics, diverse and efficient delivery platforms for siRNA are urgently needed. Extracellular vesicles (EVs) are endogenous, cell secreted nano-vehicles which have been explored for functional siRNA delivery, yet impurities in EVs preparation and lack of efficient siRNA loading method...
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Abnormalities of the long arm of chromosome 1 (1q) represent the most frequent secondary chromosomal aberrations in Burkitt lymphoma (BL) and are observed almost exclusively in EBV-negative BL cell lines (BL-CLs). To verify chromosomal abnormalities, we cytogenetically investigated EBV-negative BL patient material, and to elucidate the 1q gain impa...
Article
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Isolation of African swine fever virus (ASFV) is a critical step towards the identification, titration, characterization, and even modification of the virus. Therefore, it is important to identify a suitable cell line that supports the efficient replication of ASFV for these purposes. This should be achieved even when starting with a low virus load...
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Targeting the molecular chaperone HSP90 and the anti-apoptotic proteins MCL1 and BCL2 may be a promising novel approach in the treatment of acute myeloid leukemia (AML). The HSP90 inhibitor PU-H71, MCL1 inhibitor S63845, and BCL2 inhibitor venetoclax were assessed as single agents and in combination for their ability to induce apoptosis and cell de...
Preprint
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Although mounting evidence demonstrated that pancreas is infected by SARS-CoV-2 the severity and pathophysiology of pancreatic COVID-19 disease are still unclear. Here we investigated the consequences of SARS-CoV-2 infection of the pancreas and the role of Placenta-associated protein-8 (PLAC8). Our data showed pancreatic damage in patients who died...
Research
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This issue contains four scientific papers exploring the Software Influence of Taheri Consciousness Fields and the Existence of the Mind at the Cellular Level.
Article
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The influence of Taheri Consciousness Fields (TCFs) as non-material/non-energetic fields on various cell lines with different morphologies has been investigated. In the present study, we used violin probability density graphs to visualize the distribution of obtained data and to establish a better interpretation about the behavior of cell lines und...
Article
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Cytotoxic activity of Galantamine HBr against human cervical adenocarcinoma cell line HeLa in different concentrations (1.875 μМ ÷ 30 μМ) was evaluated. Inhibition of HeLa cell growth after treatment with Galantamine HBr and index of cell viability were determined. From the experimental results was proven that the drug exerts cytotoxic activity tow...
Article
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Cytotoxic activity of Galantamine HBr against human cervical adenocarcinoma cell line HeLa in different concentrations (1.875 μМ ÷ 30 μМ) was evaluated. Inhibition of HeLa cell growth after treatment with Galantamine HBr and index of cell viability were determined. From the experimental results was proven that the drug exerts cytotoxic activity tow...
Article
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In continuation of previous research, the anticancer screening of formulation of herbal syrup of Vinca extract was performed. The formulation of herbal syrup was performed as per reported literature method. The anticancer cancer screening was performed on two Human cancer cell lines. These cell lines were Human Lung Cancer cell line A549 and Human...
Article
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In continuation of previous research, the anticancer screening of formulation of Herbal syrup of Turmeric extract was performed. The formulation of herbal syrup was performed as per reported literature method. The anticancer cancer screening was performed on two Human cancer cell lines. These cell lines were Human Lung Cancer cell line A549 and Hum...
Poster
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synthesis and characterization of silver nanoparticles using Myricetin. Myricetin is a well-known flavonoid applied to colorectal cell lines.
Preprint
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This protocol describes a standard procedure to generate a stable cell line for a DNAJC5 knockdown using shRNA.
Preprint
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Nuclear RNA binding proteins (RBPs) are difficult to study because they often belong to large protein families and form extensive networks of auto- and cross-regulation. They are highly abundant and often localize to condensates with a slow turnover, requiring long depletion times or knockouts that cannot distinguish between direct and indirect or...
Preprint
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The CRISPR-Cas nickase system for genome editing has attracted considerable attention owing to its safety, efficiency, and versatility. Although alternative effectors to Cas9 have the potential to expand the scope of genome editing, their application has not been optimized. Herein, we used an enhanced CRISPR-Cas12a nickase system to induce mutation...
Preprint
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This protocol is being used to test the antibody , as well as . Please note that after multiple rounds of optimization, anti-LRRK2 antibody generates significant non-specific signals in the LRRK2 KO cell lines so this antibody may not be reliable for Immunofluorescence staining experiments. The protocol can be used for general IF experiments.
Article
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This study was designed to investigate the correlation between the impulse by dielectrophoretic force applied inside a dividing cell during alternating electric fields therapy and the inhibition of cell proliferation. Distributions of the electric field and dielectrophoretic force inside a dividing cell were calculated using the finite element meth...
Article
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Objetivo: Estandarizar la prueba de microneutralización (MNT) para detección de anticuerpos neutralizantes contra el virus dengue serotipo 2 (DENV-2) en la línea celular Vero-76. Materiales y métodos: Se evaluaron diferentes concentraciones celulares (0,6 x105 cel/mL, 0,9 x105 cel/mL, 1,2 x105 cel/mL), porcentajes de 2%, 3% y 4% de suero bovino fet...
Research
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problem and understanding of cells and lines = Cell line = Matrix.
Preprint
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Here we describe a new public pharmacogenetic (PGx) annotation database of a large (n=3202) and diverse biospecimen collection of 1000 Genomes Project cell lines and DNAs. The database is searchable with a user friendly, web-based tool ( www.coriell.org/StarAllele/Search ). This resource leverages existing whole genome sequencing data and PharmVar...
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Aim: Ovarian cancer is a serious malignancy with high prevalence and mortality. Methods: We isolated and characterized an ovarian high-grade serous cancer cell line (M4) from a tumor of a Vietnamese patient with ovarian carcinoma. Results: The M4 cancer cell line showed good proliferation and stability in culture. Morphologically, the M4 cells...
Poster
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#Colorectal#Cancer#HT-29drug adopted_cell_lines 5-FU Oxaliplatin
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Numerous mechanisms have shown that long noncoding RNAs (lncRNAs) promote the development of colorectal cancer (CRC), but the role of lnc-LRRTM4 in the progression of CRC remains unclear. In this article, we found that lnc-LRRTM4 was highly expressed in CRC tissues and cell lines and that lnc-LRRTM4 could promote the proliferation and metastasis of...
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Four new meroterpenoids, Clavilactone M-P, possessing novel aminoglycoside moiety (1-4) and a 10-membered carbocycle fused with an α,β-epoxy-γ-lactone, were isolated from Clitocybe clavipes, a basidiomycete. Their structures with absolute configurations were determined by extensive analysis of their spectroscopic data, and the ECD method. All the i...
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La ateroesclerosis es una patología multifactorial de progreso lento y silente en el que intervienen las células endoteliales, células musculares lisas vasculares, adipocitos, células inflamatorias y el flujo sanguíneo. Los microRNAs (miRNAs) son reguladores esenciales de las vías metabólicas inmersas en el desarrollo de esta patología por lo que s...
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Since its inception, primary retinal cultures have been an in vitro tool for modeling the in vivo environment of the retina for biological studies on development and disease. They offer simple and controlled experimental approaches when compared to in vivo models. In this review we highlight the strengths and weaknesses of primary retinal culture m...
Preprint
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Background At present, the clinical diagnosis and treatment of pancreatic cancer (PC) are challenging, and there are still in gaps in the knowledge about its malignant progression. In particular, there is not enough information about the mechanism by which exosomes released by PC cells are significantly increased and involved in promoting malignant...
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Circular RNAs (circRNAs), covalently closed RNAs that originate from back-splicing events, participate in the control of several processes, including those that occur in the development of pathological conditions such as cancer. Hereby, we describe circAFF1, a circular RNA overexpressed in alveolar rhabdomyosarcoma. Using RH4 and RH30 cell lines, a...
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Advantages of cloned Hoxb8 neutrophil-like cells are discussed and contrasted with weaknesses of human HL-60 and PLB-985 neutrophil-like cell lines, and shared and distinct features of primary murine and human neutrophils are summarized.
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The ITM2B-related retinal dystrophy (ITM2B-RD) was identified within patients carrying the autosomal dominant variant [c.782A > C, p.(Glu261Ala)] in ITM2B from whom induced pluripotent stem cell (IPSC) lines were previously generated. Here, we report the generation of three isogenic control iPSC lines from the derived affected subject cell line (IT...
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Finding cancer-driver genes has been a central theme of cancer research. We took a different perspective; instead of considering normal cells, we focused on cancerous cells and genes that maintained abnormal cell growth, which we named cancer-keeper genes (CKGs). Intervening CKGs may rectify aberrant cell growth, making them potential cancer therap...
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The dynamics of some karyotype characteristics throughout the spontaneous neoplastic evolution of embryonal fibroblasts of different mouse strains was studied using a differential staining of chromosomes. The independent variability of chromosome modal numbers, the number of polyploid cells in the cell line, the number of chromosomal rearrangements...
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Reactive oxygen species (ROS) homeostasis is crucial for leukaemogenesisand deregulation would hamper leukaemic progression. Although the regulatory effects of RUNX1/ETO has been extensively studied, its underlying molecular mechanims in ROS production in t(8,21) AML is yet to be fully elucidated. Here, we report that RUNX1/ETO could directly contr...
Poster
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The human cell line HCT-8 has been extensively used in studies to evaluate different aspects of CRC, including genetic predisposition and antitumor drug development. However, no genomic characterization of this cell line has been performed so far. Thus, in order to confirm the occurrence of genomic changes in HCT-8 that validate it as a model for s...
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Latar Belakang: Kanker payudara merupakan jenis kanker dengan angka kejadiannya menempati posisi pertama terbanyak di Indonesia dibandingkan dengan jenis kanker lain. Daun sirsak yang mengandung acetogenin memiliki efek sitotoksik terhadap sel kanker payudara T47D. Objektif: Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun sirsak te...
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The mutagenic potential of Terminalia arjuna hydroalcoholic bark extract was evaluated using Ames test with strains TA98, TA100, TA102, TA1535, and TA1537. The genotoxic potential was assessed by performing Chromosomal Aberration test with cell lines of CHO. In the assessment of mutagenic potential by the Ames test, hydroalcoholic extract of Termin...
Article
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The mechanisms by which DNA alleles contribute to disease risk, drug response, and other human phenotypes are highly context-specific, varying across cell types and different conditions. Human induced pluripotent stem cells are uniquely suited to study these context-dependent effects but cell lines from hundreds or thousands of individuals are requ...
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Herein, we report the design and synthesis of novel 7-aza-coumarine-3-carboxamides via scaffold-hopping strategy and evaluation of their in vitro anticancer activity. Additionally, the improved non-catalytic synthesis of 7-azacoumarin-3-carboxylic acid is reported, which features water as the reaction medium and provides a convenient alternative to...
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Objective: To estimate the anticancer activity of ethanolic extract of Zostera Marina. Materials and Methods: Insilico toxicology was evaluated by PASS. The anticancer activity of Zostera marina lamouroux was evaluated by MTT assay against Human colorectal carcinoma cell line (HCT116). Results and Discussion: Insilico toxicological results are show...
Preprint
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Proteomics of Wilm's tumor and Neuroblastoma cell lines
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NF2 (moesin–ezrin–radixin-like [MERLIN] tumor suppressor) is frequently inactivated in cancer, where its NF2 tumor suppressor functionality is tightly coupled to protein conformation. How NF2 conformation is regulated and how NF2 conformation influences tumor suppressor activity is a largely open question. Here, we systematically characterized thre...
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Here it is mathematically shown that higher-order polynomials are needed to have a single formula that describes the survival of all cell lines at all dose ranges, that the Linear Quadratic model is insufficient, and that the β parameter of this model is dependent on the dose range used for curve fitting. Based on the Taylor series and two mathemat...
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Propolis as natural resources from bee has complex biological and chemical depending the plant around the hives. This study aimed to find out cytotoxic activity of n-hexane fractions of propolis from Madu Efi’s bee farm N-hexane and the maceration method were used to make the fraction. Using the MTT method on WiDr cell lines for 24 and 48 hours, cy...
Article
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Here it is mathematically shown that the Linear Quadratic model is insuffcient to adequately describe the survival curve of some cell lines, and that the β parameter of this model is dependent on the dose range used for curve fitting. Therefore, higher-order polynomials are needed to have a single formula that describes the survival of all cell lin...
Article
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The objective of this study was to examine the cytotoxic effect of Barleria longiflora L.f stem extract on cervical cancer (HeLa) cell lines. HeLa cells were cultured in EMEM medium and incubated with different concentrations (12.5, 25, 50, 100 and 200 µg/ml) of ethanol extract of Barleria longiflora L.f. The method used for this research to determ...