Cell Line - Science topic

This study aimed to elucidate the immunosuppressive characteristics of Cholangiocarcinoma or CCA by investigating the role of culture supernatants from four selected CCA cell lines, representing two major types of CCA (intra and extrahepatic CCA), on the induction of M2-polarized macrophages. The specific signaling molecules and underlying mechanis...

This protocol details steps taken to create stably expressing cell lines in RAW 264.7 cells.

This protocol details steps taken to create KO cell lines in RAW 264.7 cells.

One of a cell's behavioural characteristics is migration. Cell migration is a major contributor to cancer-related mortality and plays a significant role in the spread of metastatic tumours. The purpose of this study was to evaluate the invasive characteristics and migration traits of acquired gemcitabine-resistant BT 549 Gem100nM breast cancer cell...

These authors contributed equally to this work. Abstract: Methionine is an essential amino acid. Dietary methionine restriction is associated with decreased tumor growth in preclinical studies and extended lifespans in animal models. The mechanism by which methionine restriction inhibits tumor growth while sparing normal cells is not fully understo...

BACKGROUND Currently, intrahepatic cholangiocarcinoma (ICC) poses a continuing, significant health challenge, but the relationship has yet to be established between ICC and the proteasome 26S subunit non-ATPase 6 (PSMD6). AIM To investigate the protein expression and clinicopathological significance of PSMD6 in ICC. METHODS The potential impact of...

Human essential proteins (HEPs) are indispensable for individual viability and development. However, experimental methods to identify HEPs are often costly, time consuming and labor intensive. In addition, existing computational methods predict HEPs only at the cell line level, but HEPs vary across living human, cell line and animal models. Here we...

Los agentes quimioterapéuticos Doxorrubicina y el Paclitaxel han sido cuestionados desde hace algún tiempo, por los efectos negativos observados en algunos pacientes, cuando se usan en concentraciones altas. Sin embargo, estudios han demostrado que productos de origen natural muestran sinergia con estos fármacos reduciendo la dosis. En función de e...

Molecular hybrids of diversely functionalized quinoline and sulfonamide have been designed. Multistep synthetic strategies have been used for the synthesis. The anti‐cancer properties have been evaluated against various cancer cell lines including HCT116, A549, U2OS, CCRF‐CEM, Jurkat, MOLT‐4, RAMOS, and K562. Non‐cancer cell lines MRC‐5 and BJ were...

To improve our understanding of multi-drug therapies, cancer cell line panels screened with drug combinations are frequently studied using machine learning (ML). ML models trained on such data typically focus on predicting synergy scores, which support drug development and repurposing efforts but have limitations when deriving personalized treatmen...

The capacity of the F3K Digitalis purpurea cell line to biotransform hydroquinone into β-arbutin was tested. The experimental protocol involved the sequential addition of hydroquinone to the culture medium, and testing different precursor (4-, 5-, and 6 mM) and sucrose concentrations (3 % and 6 %) under different lighting conditions (16-hour photop...

Independently of the pathways or circuits deregulated in cancer cells, these present altered transcription patterns, often also direct consequence of deregulation of transcription factors. In this sense, also the RNA Polymerase complexes responsible for transcription can be affected in cancers. We find that upregulations of RNA Polymerase II subuni...

A complex study regarding biomechanical features of spheroids generated from different glioblastoma cell lines. The assays have been designed to obtain whole knowledge of this kind of three-dimensional cell culture; and potential applications in cancer treatment are also explored.

Production of pseudotyped virus an subsequent transduction.

The method utilized is Trans well migration assay.

the effect of pomegranate extract on mcf-7 cell lines ( genetic researches)

the effect of pomegranate extract on mcf-7 cell lines and lymphocyte (genetic research)

La implantación de una célula maligna o de una colonia metastásica indica indefectiblemente la respuesta combinada de las células tumorales y de las células sanas y es la consecuencia final de un proceso activo, continuo, complejo y multiescalonado denominado Cascada metastásica. Uno de los puntos importantes es detener este proceso antes de que se...

Targeted therapeutics for high-risk cancers remain an unmet medical need. Here we report the results of a large-scale screen of over 11,000 molecules for their ability to inhibit the survival and growth in vitro of human leukemic cells from multiple sources including patient samples, de novo generated human leukemia models, and established human le...

This protocol describes the metabolic labeling of mitochondrial translation in cultured cells (such as IMR90) using an amino acid L-Homopropargylglycine (L-HPG).

Here we update our previous poster by adding additional data that suggests that some ERBB4-dependent BRAF WT melanoma cell lines are also dependent on ERBB2. Moreover, we present data indicating that one ERBB4-dependent BRAF WT melanoma cell line is independent of EGFR and ERBB2. We postulate mechanisms for differential dependence on ERBB2 among ER...

Understanding the relationships among genes, compounds, and their interactions in living organisms remains limited due to technological constraints and the complexity of biological data. Deep learning has shown promise in exploring these relationships using various data types. However, transcriptomics, which provides detailed insights into cellular...

Here, we describe protocols for analysis of the inducible protein aggregate agDD-GFP in HeLa-dCas9 and HEK293T cells under a variety of conditions and in various genetic backgrounds. agDD-GFP is stabilized by a small molecule (shield-1, S1) and washout of S1 leads to agDD-GFP aggregation in minutes. By monitoring aggregates after S1 washout by flow...

Background Few studies have explored the expression profile of RPL3 in breast cancer (BRCA). Our research provided an in-depth analysis of RPL3 expression patterns in BRCA, highlighting its significance for therapy prediction and prognosis. Methods RPL3 was notably elevated in malignant cells, and its expression level was closely associated with t...

Allele-specific, monoallelic expression in diploid organisms represents an extreme case of allelic imbalance resulting from incompatibility between cis- and trans-elements. Due to haploinsufficiency, such monoallelic expression can lead to sporadic genetic diseases. In mice, allelic imbalances can be introduced into F1 offspring from inbred strains...

Introduction Bladder cancer is the ninth most common tumour entity worldwide. Aberrant expression of different keratins has been described in bladder cancer, which is used for diagnostic purposes, but it can also have prognostic value. However, not all keratins have been analysed in bladder cancer, and whether keratins are important for cell viabil...

In the present investigation urea-based small molecules with unsymmetrical substitutions were synthesised and their in vitro cytocoxic activity was assessed against Human breast cancer (MCF7) cell lines. The title compounds (UDa-UDl) were synthesised in two steps and their in vitro cytotoxic activity was evaluated using MTT assay method. All the co...

Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase‐based reporter cell line capable of detecting the activities of IFN‐Is, IFN‐II, and IFN‐IIIs. The reporter cell line exhibits a 12...

Human induced pluripotent stem cell-derived models are a well-established preclinical tool, with the ability to retain the genetics of the individual from which they are derived. Here we comment on the global representation and accessibility of such cellular tools from African population groups.

Precancerous cells are present in all human bodies. Various environmental triggers can promote the development of cancer. Microplastics, an emerging concern, may potentially act as one such trigger, contributing to cancer initiation or progression. Studies have confirmed the presence of microplastics within the human body. This raises concerns abou...

Background: First-generation anti-SARS-CoV-2 monoclonal antibodies (mAbs) used for prophylaxis or therapeutic purposes in immunocompromised patients have been withdrawn because of the emergence of resistant Omicron variants. In 2024, 2 novel mAbs, VYD222/Pemivibart and AZD3152/Sipavibart, were approved by health authorities, but their activity agai...

The editing efficiencies of prime editing (PE) using ribonucleoprotein (RNP) and RNA delivery are not optimal due to the challenges in solid-phase synthesis of long PE guide RNA (pegRNA) (>125 nt). Here, we develop an efficient, rapid and cost-effective method for generating chemically modified pegRNA (125–145 nt) and engineered pegRNA (epegRNA) (1...

Proteins operate within dense interconnected networks, where interactions are necessary both for stabilising proteins and for enabling them to execute their molecular functions. Remarkably, protein-protein interaction networks operating within tumour cells continue to function despite widespread genetic perturbations. Previous work has demonstrated...

Deciphering cellular responses to genetic perturbations is fundamental for a wide array of biomedical applications. However, there are three main challenges: predicting single-genetic-perturbation outcomes, predicting multiple-genetic-perturbation outcomes and predicting genetic outcomes across cell lines. Here we introduce Subtask Decomposition Mo...

This poster demonstrates the development of drug-resistant cancer cell lines and their application in cancer research.

Natural killer (NK) cells are candidates for adoptive cell therapy, and the protocols for their activation and expansion profoundly influence their function and fate. The complexity of NK cell origin and feeder cell cues impacts the heterogeneity of expanded NK (eNK) cells. To explore this, we compared the phenotype and function of peripheral blood...

Despite extensive research, the molecular role of AGR2 in the progression and metastasis of colorectal cancer (CRC) has not been fully characterized. We used quantitative mass spectrometry (SWATH MS) to identify differentially expressed proteins in paired CRC cell models of the SW480 and SW620 cell lines in response to AGR2 protein level manipulati...

Objectives Ionizing radiation is still one of the most effective treatment options for various cancers. It is possible to reduce the side effects of this effective treatment method and increase the chance of success by elucidating the responses it creates at the molecular level in the cell. This study aims to investigate of the molecular effects of...

The following study was conducted to generate a transgenic Sf9 cell line for rapid and easy virus quantification in the baculovirus expression system (BES). The hr3 (homologous region 3) and 39K and p10 promoters were used as the expression structures to induce rapid and intense expression of the enhanced green fluorescent protein gene in cells in...

Background Chromosome organization plays an important role in biological processes such as replication, regulation, and transcription. One way to study the relationship between chromosome structure and its biological functions is through Hi-C studies, a genome-wide method for capturing chromosome conformation. Such studies generate vast amounts of...

A series of 6 small PAMAM dendrimers (G0-dendrimers) differing in the size, polarity and flexibility has been synthesized. The toxicity has been investigated in three different human cancer cell lines (HeLa, MCF-7, THP-1) and the endothelial skin cell line HMEC-1 in order to evaluate their potential as vehicles for drug delivery.

Here, we describe a transwell assay using Boyden chambers as an indirect co-culture method, where two cell lines are grown together but separated by a porous membrane. This assay was used to determine the effect of hypoxic, tumoral EVs in cells from the tumor microenvironment without direct contact between them, focusing on intercellular adhesion a...

Введение. Один из возможных механизмов метастазирования, лежащий в основе онкогенеза меланомы представляет собой изменение экспрессии профиля miRNAs. Характер экспрессии miRNAs в клеточных линиях меланоцитов меланомы оцениваются как потенциальные биологические маркеры этапов метастазирования меланомы. Цель. Определить в клеточных линиях меланомы ко...

Here in we describe the synthesis and characterization of a series of thiosemicarbazone platinacycles. Their activity towards HCT116 and A2780 cancer cell lines, as well as normal fibroblasts was explored...

This study investigated the applicability of the area of spheroids and hypoxic regions for efficient evaluation of drug efficacy using machine learning (ML). We initially developed a high-throughput detection method to obtain the area of spheroids and hypoxic regions that can handle over 10 000 images per hour with an error rate of 2%–3%. The ML mo...

Extensive research into platinum-based chemotherapeutics has been underway for decades with ruthenium-based complexes emerging as interesting and potent candidates. Even still, there is no evidence of a single mechanism of action across all synthesized and tested Ru-based complexes, prompting the continuance of research in this field. In addition,...

Prostate cancer (PCa) is the leading cause death from cancer in men worldwide. Approximately 30% of castrate-resistant PCa become refractory to therapy due to neuroendocrine differentiation (NED) that is present in <1% of androgen-sensitive tumors. First-in-class imipridone ONC201/TIC10 has shown clinical activity against midline gliomas, neuroendo...