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Caving - Science topic

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A flexible pavement/bitumen road alongside an unlined water canal is damaged. the road is caving in as the soil underneath it at the embankment end start flowing towards the slope of the channel and flood level of water in the channel has also risen due to heavy rains. Now the soil underneath at the near end of embankment got loose and start flowing in the channel. a crack has developed along the edges of the road. What is this phenomenon called?
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Looking at the images, there are a fair few bricks along the edge. Suggests that there was some attempt to built a retaining wall along the edge of the road. Probably founded on poorly compacted fill. The rain has caused this to slump or scour away causing the loss of support to the road. This then becomes a slip failure due to loss of support.
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Hi
Does anyone know a better way of dry packing Pt-Alumina powder into a short column (used for online reduction) other than hand packing? We are suspecting the column might be caving in due to uneven packing.
Thanks!
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Hand packing is a common method for dry packing powder into a column, but it can result in uneven packing and lead to issues like caving in of the column. To achieve more uniform packing of the Pt-Alumina powder, you could try the following alternative methods:
  1. Vibrational Packing: This method involves using a vibration table or shaker to settle the powder particles into a more uniform and dense packing. The column is placed on the vibration table and vibrated at a controlled frequency and amplitude while the powder is slowly poured into the column. This method can result in a more uniform and compact packing of the powder.
  2. Centrifugal Packing: This method involves spinning the column around its central axis while slowly pouring in the powder. The centrifugal force generated during spinning causes the powder to settle towards the outer wall of the column, resulting in a denser and more uniform packing. However, this method requires specialized equipment and may not be suitable for all column sizes and shapes.
  3. Pneumatic Packing: This method involves using compressed air or gas to push the powder into the column. The column is placed vertically with a porous plate at the bottom and the powder is poured into the top of the column. Compressed air or gas is then introduced from below the porous plate to push the powder into the column. This method can result in a more uniform and compact packing, but it requires specialized equipment and may not be suitable for all powder types.
Overall, the choice of the most appropriate method will depend on various factors such as the column size, the powder properties, and the available equipment. It is important to carefully control the packing conditions to achieve a uniform and dense packing of the powder.
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Bat Conservation
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dear Anna,
in this issue "Migration Status of Bats in Ukraine" there is very interesting article by Tomasz Postawa "Migration activity of bats during hibernation"
Tomasz use loggers "OSNET". for details ask him: Tomasz Postawa
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Natural resources like streams, valleys, caves, forests etc abound in southeastern Nigeria. These ecotourist attractions are not developed and the contemporary use of these resources has many negative effects? Why has government of southeastern Nigeria not shown interest in developing these natural ecotourist attractions? Can Public-Private-partnership offer any hope in developing these attractions?
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It refers to cooperation agreements between the state and the private sector for the provision of public services. By combining the social function of the state and the profit motivation of the private sector, it provides rapid implementation of a large number of projects in the public interest. Corruption is the most discussed issue about this cooperation.
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Reading the application of muon tomography applied in caves, our group are searching for a CCC portable detector. Thanks in advance
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Dear prof. Raul Perez-Lopez,
we have developed portable CCC-based muon detectors for speleological applications in Wigner RCP, Budapest, Hungary. If you are interested in the application of this technology, please feel free to contact me. My e-mail address is the following: olah.laszlo@wigner.mta.hu
Best regards,
Laszlo Olah
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something explains cutting shapes and size with respect to borehole problems (cavings)
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Yes that is true, Thanks a lot Luigi Moroni
appreciate it.
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Recently I took a photo of this whip spider in a northern peruvian cave (1000 metres above sea level). Could you please help me to identify the Genus and Family? Thank you very much.
Greatings from Peru.
Stefan
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If it's a Heterophrynus armiger, it could be the first record in Peru?:
What do you think José A. Ochoa ?
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Our cave-dwelling bats research group in CEBS (UFLA/Brazil) is applying this concept to the bats of the caves here in Brazil, and has generated very interesting results.
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Nice! It's good to talk with someone with different experience in the same research line...
In my study site, wonderfully and peculiarly, there are so many ... but so many caves. that there are no patterns. Moreover, the dominance of a few common and too many rare species made the beta diversity equivalent to turnover and complementarity (Cardos et al., 2012). Combined with the intense nocturnal visitation is as if all caves were one. Like a complex network linking different species, their behaviors, among various food availabilities, competition intensities, and so on...
In fact, these are the same variables that you will analyze right? How are you thinking to colect this data? I always take into thinking that to spend time and money to limit other features (besides the cave), I need to know how the dynamics of it all works. Sorry, I sometimes get excited!
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Does anyone know where is it possible to find Hazelton's papers on cave fauna from Great Britain in pdf format?
Hazelton, M. (1955) Biological Supplement of the Cave Research Group of Great Britain. Part 1 (1938–39).Cave Research Group Newsletter 52
Hazelton, M. (1956) Biological Supplement of the Cave Research Group of Great Britain. Part 2a (1940–46).Cave Research Group Newsletter 58-59
Hazelton, M. (1956) Biological Supplement of the Cave Research Group of Great Britain. Part 2b (1940–46).Cave Research Group Newsletter 60-61
Hazelton, M. (1958) Biological Supplement of the Cave Research Group of Great Britain. Part 3 (1947).Cave Research Group Newsletter 72-77
Hazelton, M. (1959) Biological Supplement of the Cave Research Group of Great Britain. Part 4 (1948–49).Cave Research Group Newsletter 79-80
Hazelton, M. (1960) Biological Supplement of the Cave Research Group of Great Britain. Part 5 (1950–53).Cave Research Group Newsletter 81
Thanks in advance!
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Hello,
I have some calcite speleothem samples which I believe are certainly older than 2 Ma, probably older than 5 Ma (a cosmogenic 26Al/10Be dating of a sediment from a lower cave gave an age of 4,2 Ma). I am looking for a laboratory able to date these samples with the U-Pb method. Can you suggest me some laboratories ?
With many thanks in advance.
Miguel Borreguero
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We do speleothem dating at MIT by both the U-series method (McGee Lab) and the U-Pb method (Bowring Lab). It is the isotope dilution technique using a combination of MC-ICPMS and TIMS instruments.
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Hi,
Is there anybody who has any knowledge about the Lepidopahtogenic Fungi? This butterfly was found in a cave. It was covered with this pathogenic fungal body. (Pictures are attached.)
Thank you in advance for your attention.
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Cordyceps sinensis was shown in 2007 by nuclear DNA sampling to be unrelated to most of the rest of the members of the genus; as a result it was renamed Ophiocordyceps sinensis and placed in a new family, the Ophiocordycipitaceae, as was the "Cordyceps unilateralis" Other species previously included in the genus Cordyceps have now been placed in the genus Tolypocladium.[citation needed]
Cordyceps and Metacordyceps spp. are now thought to be the teleomorphs of a number of anamorphic, entomopathogenic fungus "genera" such as: Beauveria (Cordyceps bassiana), Lecanicillium, Metarhizium and Nomuraea.[citation needed]
When a Cordyceps fungus attacks a host, the mycelium invades and eventually replaces the host tissue, while the elongated fruit body (ascocarp) may be cylindrical, branched, or of complex shape. The ascocarp bears many small, flask-shaped perithecia containing asci. These, in turn, contain thread-like ascospores, which usually break into fragments and are presumably infective.
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Hi all,
I am hoping to get some help with identifying the particles in the attached images. All photos were taking in polarized light, scales should be on the images.
A bit of context: the thin sections are of a speleothem sample from the south coast of South Africa. The cave is a sea cave in a granite host rock. The source carbonates for the speleothem are calcretes and aeolianites on top of the granite.
Most of the sample is Calcite with a microcrystalline to micritic fabric. Besides the particles in question, there is a lot of fine sand-sized quartz in the sample. I am suspecting that the cave was partly open when this speleothem formed (ages are Holocene 7.8 ka- 0.7 ka BP). The sand may have been washed in by high waves from the beach and the particles could originate from within the cave, host rock, or from the beach/shoreline environments. The color made me think it was maybe something organic, but It doesn't have any cell structures as wood or other plant materials would have. It also doesn't seem to be bone. I would be grateful for any help.
Kerstin
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Daar Kerst in,
Not sure about the clay. The extinction pattern does not seem to match. I woud hazard they may be Ca, Fe(III) phosphates. They are not always fluorescent, so you may need to check wit SEM-EDX, micro-XRF or similar techniques.
All the best,
Hans
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Can someone tell me whether any other species make drawings or effigies of themselves (or of things in their environment)? I am aware that many species recognize familiar patterns. But do any other species advertently "make" patterns - to induce responses unnaturally (to compel recognition)? Thank you for any help.
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Usually creating artifacts of one kind or another is considered a specifically human trait, whether creating an axe head or a clay pipe. Utilisation does not necessarily exclude art as pointed out. A mental map tends to be involved, plus use of hand and eye.
Although we now know that many animals use tools, and some shape them, invariably they serve single contingent purposes. (Does that though exclude them completely from art?)
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I am doing research for a mercury-related consultancy and a national inventory of mercury sources and releases in Central America. During the research, I have collected informal evidence that there are some 'natural' sources of metallic mercury (not cinnabar) in caves and certain areas with high volcanic activity. According to the interviews and informal chats that were conducted, these sources / deposits of mercury have been known since decades and metallic mercury has been traded illegally during years. I have reviewed different information sources, databases and books and I'm unable to find information whether metallic mercury (liquid mercury) can be found in nature (yes, the silver liquid flowing) or not.
Do you have any idea about this topic? Thank you.
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Hi Julio,
Yes, liquid natural elemental mercury does occur. A well known source is the production of elemental Hg with natural gas production which then condenses. A lesser known source is the production of elemental mercury during in (deeply) anoxic aquifers where Hg2+ (and Hg+ for that matter) can start to act as an electron acceptor. This process results in the production of liquid elemental mercury.
Hope this helps! If you're interested in the flow behaviour of elemental liquid mercury in soils/aquifers, I have a few recent articles on the topic in my profile.
Best regards,
Niels
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Looking at the Invertebrate Taphonomy within a cave system to help address questions of human evolution. The caves used are frequented by hyena and I would like to prevent them from accessing the carcass so only Invertebrate caused damage occurs.
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Thank you for that Mesfin. I will keep that in mind.
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I've already started my sampling for Philippine tarantula species, and so far, what I have encountered are mostly terrestrial tarantulas that are found near riparian areas and those in caves, sometimes semi-arboreals too, those of which that have tunneled in tree holes 1-2 ft from ground (tunnels have signs of continuing down to the ground). I aim to find bigger species of tarantula ( my samples range from small to very tiny) for the purpose of my study, and I heard that "Arboreals" are mostly bigger. Even so, it is really difficult to find them in the wild and most of my sampling are conducted during the day, I also tried sampling twice at two different locations-- agroeco and secondary forest during night time , but I must say it was equally difficult, if not, then more. I hope to gain knowledge on how to spot them quickly and so if anyone can share if there are any efficient traps that you know of or any factors ( elevation, time of the day, preferred vegetation, habitat preferences etc.) that you think I have to consider as well, any at all, I would like to ask them from you, it would so much help me in my study. Thank you very much! :)
Note: Philippines is a tropical country ^^
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Flight interception traps sometimes also have arboreal tarantulas inside
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The local Earth magnetic field deformation is an efficient approach to identify the localisation of heart if the sediment contain iron even in trace concentration. The recent methodology development done for the Fraux cave and used in the Bruniquel cave allow to obtain very nice data. This approach is also efficient for more classical archaeological excavation site such yours.
regards
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Fire i.e. the temperature elevation above +/- 250°C of sediments or soils materials generate iron phases transformations (if weakly stable phases contemning iron are present) and so magnetic minerals enhancement. This magnetic minerals enhancement can be monitoring with contact sensors for magnetic susceptibility or magnetic viscosity measure. Thermoremanent magnetization is also acquired. This remanent magnetization and the induce magnetization generate by the local earth magnetic field are the origin of the local deformation of the magnetic field. Unfortunately, this signal is low compare to iron artefacts. For prehistorical fire, the signal is often undetectable beyond 1 m distance of the source, even before. So it is important to make survey very close to the surface. In such condition, it is difficult to obtain significant signal with magnetometer carried by UAV. The best is to measure total field variation. For this purpose (total field), in my opinion, optical pumping magnetometer are the most adapted for archaeology survey nowadays. The advantage geomagnetic survey compare to magnetic susceptibility is that you can detect sources even if it is covered by few decimeter of materials.
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If time’s dimensionality is similar to or the same as the dimensionality of a direction in 3 dimensional space, then what is the underlying nature of time? Minkowski’s space time concept implies that the 4th dimension (which he assigns to time) should be treated as just one more of 4 dimensions for a 4 dimensional metric. The 4/3 law implies the same thing particularly with respect to the equipartition of energy. The 4/3 law appears to account for dark energy. Is time a shadow (of the underlying reality) cast on the cave wall? If it is, what is it a shadow of? Expansion of the universe?
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 In the theory of relativity, we define an observer who has two instruments; one for measuring the length and the other for measuring the time. The demand of the principle of relativity then implies that all possible observers must have the identical instruments. In the Einstein's theory of relativity , the instrument measuring time has one important role; that is to measure the time' t' taken by a light ray to reach the point (x,y,z) from the origin(o,o,o). It is this time 't' which   defines the forth coordinates of the four dimensional space-time in the Einstein's theory (special and general ) relativity.
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I am looking for the published works having any data on fishes in marine caves outside the Mediterranean i.e. in any other sea. Looks very scarce. I would be very grateful for the pdfs or complete references.
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Sorry..
It looks very scarce.
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I think I would be necessary to start a Field-Project on the Caves of southern Catalonia with Bronze Age Occupation  (Cova del Janet, etc.) studying Morphology, Space, Geological Features using modern Techniques. There are excellent older Studies written by Salvador Vilaseca, but as an Arcaheologist studying the Pottery of these Sites, I would like to know how these Places are like and how they might appear in tridimensional Computer Views.
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La verdad es que yo no trabajo sobre este período, quizás le interesaría contactar con Jordi Diloli, profesor de la Universitat Rovira i Virgili de Tarragona, quien quizás podría ayudarle.
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If you knew the steady-state 222Rn concentration in cave air, i.e., the maximum concentration that would develop in the cave if there were no air exchange, then from that, plus measurement of the rate of cave venting, the 222Rn concentration of vented air, and the time (about 19 days) it takes 222Rn in air to reach steady state with the surrounding rock, you could calculate a minimum total volume of air in the system. If measuring the steady state concentration were difficult of impossible (as it probably would be), you could get the same information by looking at changes in 222Rn verses changes in air venting rate, on seasonal to annual timescales. This is not a project I am interested in, but I thought I’d throw the idea out for discussion in case someone else is.
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Dear Joseph,
I know of no one  publshing an article concerning using 222Rn to estimate/calculate the volume of a karst system.  
One probable problem for not using this method immediately comes to mind.  Its the specific gravity of 222Rn vs that of air ( ~29).  As you can see, by the difference between these two numbers, your Rn atoms would act as if they were small, lead, B.B's  They'd sink thru the air column like small rocks.
Even CO2, with a molecular weight of but 44, quickly settles and layers along the floor of caves.  Thus, we get a very few caves - containing "Bad Air".  222Rn would settle even faster.
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Instead, you can calculate the volume of Karst systems - using gravity waves, diurnals, and last using the High/Low barometric pressure cells that cross over the U.S.A.
Gravity waves, in the atmosphere, tend to be caused by the Jet Stream, or major storms  These are low amplitude pressure waves - having a short period normally about 10 to 60 seconds long.  It takes a good micro-barometer, with a detection capacity of from 0.01 to 0.1 mm Hg, to catch and measure them.  An article on how to make a home made "Speleobarometer" was publised in the National Speleological Socities "Bulletin" about 1955-58.  
I used the data from that article - to calculate the minimum volume of Breathing Cave, Va. As I remember, the cave has a barometric volume somewhere between 20 -40 million cubic feet.  Gravity waves cycle, but they are not repeatable.  They vary in both amplitude and also period length - shiftying character even as often as every half cycle.
The diurnal is a longer pressure event - caused by the sun heating the land and atmosphere, during the day.  This heat expands the atmosphere, and lowers the barometric pressure by several mm of Hg.  Then, when the sun sets, the land and atmosphere cool off - causing the atmosphere to contract and increase in pressure.
At Lechuguilla Cave, N.M. this effect generated barometric cave winds of +/- 10-15 mph.  ... in roughly a 24-hr cycle.  Using the Ideal gas law, I calculated that Lechuguilla Cave had a minimum volume of 1.5 Billion cubic feet.
Lechuguilla Cave, though, responds much better and stronger - to the High and Low pressure cells that move across New Mexico.  I put together an expedition to measure the cave responding to these deeper, stronger pressure cycles.  
Lechuguilla Cave initially was measured sucking in at 43 mph.  The barometric wind then slowed, stalled, and reversed direction blowing out.  It blew out for 84 hours before slowing, nulling and once again reversing.  During that time the average wind velocity blowing out was 33 mph.  (It hit a peak velocity blowing out at over 49 mph.)
Using the Ideal Gas Law, Lechuguilla Cave has a minimum Karst system volume of 3.2 billion cubic feet.  By using its nominal 200 square feet of cross section - of its 'Halls", this cave could be as long as 2,400 miles.  Currently Lechuguilla is the 7'th longest surveyed cave in the World - at 138.3 miles in surveyed length.  (www.caverbob.com/wlong.htm)  Its calculated to be hidden within 45-square miles of land.
The largest Karst cave system currently known is - "The Black Hills Cave System" of South Dakota.  This cave system is believed to 'ring' the Black Hills anticline uplift, hidden within the Pahasapa Limestone.  The southern section has two entrances - lying within Wind Cave National Park, and Jewel Cave National Monument.  (Herb Conn, "Barometric Cave Winds of Wind and Jewel Cave" published about 1966 in the National Speleological Society Bulletin".)
These two entrances lie 25 miles apart.  The minimum volume of this southern section of Karst System is 4.5 billion cubic feet.  It is estimated now that the cave surveyors of these two caves have surveyed but 3% or so of the entire cave.  Jewel Cave, with a current surveyed length of 181.89 miles - is the 3'rd longest surveyed cave in the World; while Wind Cave, with a current surveyed cave length of 142.75 miles - is the 6'th longest surveyed cave in the World. Thus, this section of the cave system could be over 7,000 miles long, underlying 145-square miles of Minnelusa Sandstone caprock.
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Outside the U.S.A, the only other place I know of that have caves with measurable barometric winds - are those in S.W. Australia, East of Perth.  The "Aussies" call them, "Barometric Breathers".  Some of these caves have very strong cave winds - but it appears to result more from having a  "very porous" limestone.   
-Bruce Zerr
Director
Tennessee Cave Survey
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1950s-1990s, esp excavation methods for open air and cave sites.
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There's also Marc Groenen's Pour une histoire de la Préhistoire which provides a non-exhaustive summary of the history of excavation methods.
And some more about Leroi-Gourhan's techniques by Paul Courbin (1987):
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I have a flac3D model of coal mine. The panel is of 200*200m size. Boundary of model taken to be 200m away from boundary of the panel on all sides. Progressive face advance is being carried out alongwith caving. How can I be sure that boundary effects are not altering my stress distribution?
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Increase the size and do several parametric study to ensure the stress distribution doesn't change with the model size. 
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The first signs of art date from more than 30.000 years ago (e.g. cave art) whereas the first signs of writing (e.g. Tamil) appeared let say ca. 5000 years ago?
Any idea why artistic humans waited such a long period before they decided to start writing?
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Andras,
Yes, until the 19th/20th century in most of the world, reading and writing was used by the elite of society (politicians, clergy, royalty, merchant princes) as a means of controlling the masses. As I have noted before on RG, colonial Mexico is a prime example where baron ranchers and other powerful people would go to "university" in order to learn enormous and complex words in which to use in front of their workers as a means to show the "peons" how much more intelligent and worthy of leading the "commoners" they were.
I believe that this has been propagated by some university systems even now, when you get questions like "What is macrogamete?" or "Define staphylorrhaphy" or "What is flagitiousness?" on college GRE exams. As if the everyday person, even with a minor degree, knows these obscure words, much less needs to use them in everyday conversation.
But getting back to the question - as previously noted, writing arises when a society reaches a certain technological/social complexity. Some will use the Inca as argument against this, but the Inca are almost unique, for they used knots on cordage/string to convey words. Thus, like cuneiform, their "writing" used a system of relationships between the same basic element to convey letters/words. The need to count, to keep track of numbers/inventory, is seen by many to have been the basis for the rise of the written language in several cultures. Some of the first "writing" that we find are marks on clay tablets, some with stamps/seals to show author.
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I found it near Petrota village under stones. As I know its type locality is Maroneia cave and species was described by Riedel in 1985. Can anyone tell me where I can find a PDF of this paper?
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Dilian,
I don't have the answer to this, but I am guessing that the type specimens will be in Riedel's collections in Warsaw (the actual collections have been moved to repository outside the city.) Try contacting Beata Pokryszko at Wroclaw, who has used the collections to deal with Caucasian material. I am not sure of the etiquette of posting email addresses online, but if you cannot find her through University of Wroclaw webpage, email me through researchgate. She may well have a copy of the original Riedel 1985 paper in Malak. Abh. Dresden, 10: 139-147. I am sure this will give the details. I think Wroclaw has a complete run of this journal.
 I will be in London on Feb 3, and if I have time, I will check the holdings there.
My guess is that it was collected by Riedel himself, since he did much work on cave faunas in Greece.
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It was found in Morgado Superior Cave, in the Nabão valley (Central Portugal).
In this Cave we have a 195 MNI, and some artefacts, This is one of them. This place has akready 4 AMS dates from Chalcolithic.
I woul appreciate some help.
Tanks a lot Best Wishes
Ana 
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Dear Ana,
This is what is called in Portuguese "um punção losânguico" in French "allene lozanguique" typical of the Early Bronze Age. See, for example, "Senna-Martinez, J.C. 1994. "Subsídios para o estudo do Bronze Pleno na Estremadura Atlântica: (1) A alabarda de tipo «Atlântico» do Habitat das Baútas (Amadora)", in: Zephyrus, XLVI-XLVII, pp.149-170.
Best wishes,
J. C. Senna-Martinez
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Caves and karsts are unique habitat type that harbor a diverse and unique taxa but currently imperiled by human exploitation. It has been a practice among conservationist to use a surrogate taxa in conserving and protecting habitat, in caves, among the numerous taxa what would be the best candidate?
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Like Mary, I also agree with Marcelo and Kendra. Bats could provide nutrients for cave animals but that is exclusively for those coprophagous. If the cave is disturbed, the bats could easily move from one location to another, they could easily find new suitable cave habitats. Invertebrates, which dominated the subterranean ecosystem, have limited movement, most especially the stygobitic animals (cave-obligate aquatic species). They are very sensitive even to slight cave disturbance therefore they could be affected very easily. Moreover, endemism of this stygobites are very high. So considering everything, stygobites are overall more prone to extinction. Cheers!
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I have some findings in the Bulgarian caves. Years before the Russian scientist Zhadin (1952) described the genus Spaeleopisidium from the caves.
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Hi David, Many thanks! In Bulgaria till now I found only Pisidium personatum, but some forms are strange... Stygobiotic snails are many species, in contrast, me described about 15 new species and even some new genera! Possibly the situation in the clams is not the same... After the Zhadin description of genus Speleopisidium from Caucasus, it was synonimized wth Euglesa by Korniushin (relatives of P. personatum)... As I said to my colleague and friend before: interesting is in front of us!
Cheers!
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I recently saw the two species by feeding, but I wonder if they can share the same caves or other shelters to sleep, reproduce and care for the offspring.
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Hi, the two species were usually recognized a competitors to some degree. There was a long history of Norway rat pushing back the Rattus rattus. However, in recent years, there are some news about regaining some territories by Black rat.  It seems possible, that one way of adaptation of the two species is the increase of mutual tolerance of the two species. They would have to work out however, the way to share the niche, or to avoid confrontation.
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For paleo-shoreline demarcation.
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Mylroie J E and Carew J I 1995 describe flank margin caves from the Bahamas formed in the saline/freshwater mixing zone--maybe helpful for what you need---paper title is: 'Karst development on carbonate islands/Speleogenesis and Evolution of Karst Aquifers 1. In Unconformities in Carbonate Strata-Their Recognition and the significance of Associated Porosity (eds. D A Budd, A H Saller and P A Harris), pp 55-76. American Association of Petroleum Geologists' Memoir no 63.
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Is anyone interested in joining a research activity including discovery and dating of ancient quarries along the path of the ancient Via Appia route (central-Southern Italy)?
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What about SISKA, Switzerland, Philipp Hauselmann: http://www.isska.ch/En/portrait/index.php?page=ph
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Can somebody tell me the proper site for speleothem sampling?
I am little bit confused whether is taken from the location where it is equilibrium with external atmosphere or deep from the cave where humidity is near about 100. Some one told me the sample collected near from the cave entrance are most significant for the climatic study because it records the diurnal variation of the atmosphere. but here the kinetic fractionation also goverened the precipitation process please tell me the exact things.
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This question almost requires a book to reply to... The short answer is: "stalagmites are considered to be isotopic recorders of the average climate of the past provided that they grew (i) in deep parts of the cave where temperature is constant and equal with the multi-annual mean temperature (MAAT); (ii) RH>95%, i.e. no kinetic fractionation; (iii) they can be dated with a resolution that matches the isotopic profiling"
This being said, forget about stalagmites in the entrance zone (unless the "entrance" is something like 20x20 cm hole which was excavated by speleologists or something like that). Also, one must understand the genesis of the cave before hammering stalagmites... What is today a "deep zone" may not have been in the past. For example, what we think is a "deep zone" may correspond to a former entrance of the cave in the past. Understanding cave genesis is paramount.
I don't want to upset anybody, but often people that work on stalagmites taken from entrance areas are not speleologists and they just grab the first stal they stumble upon. And then they come to the conclusion that equilibrium fractionation does not exist because, guess what? it isn't supposed to exist in the well ventilated areas anyway... So, go deep in the cave, choose a nice, "quiet" spot (for instance, a side passage, away from underground rivers and corresponding ventilation), then look for your stal. Hit it gently withyour hammer or with the chisel - listen to the sound: it should be high pitched. Hit many other stals until you choose one. Look at them in counter-light, try to see how nice and clear is the internal structure. If possible, take a small core from the base of the stalagmite and take it to the lab. It will give you an age estimate and (more important) the U-content, so you know what to expect in terms of age resolution.
Sometimes it is not possible to do the coring, do the preliminary date, and come back for sampling. So, you just have to use your best judgement before "hitting to kill". If there are broken stals around, look at their sections to have an idea what you can expect. If you can identify their roots, take the broken ones and hammer for the roots. If you decide to break a standing stal, document everything well. Look at the feeding point. If it's a soda straw, break also 1-2 cm of the straw tip for stable isotope analysis. If the feeding point comes from a curtain, then you will probably have lateral deviations in the profile of the stal - this is not good news, maybe you find something better. If the dripping point is very high, again this is not good news - the splash will deposit a lot of calcite on the sides of the stalagmite instead of the center, where you want it.
Ideally, what you want to get is a stalagmite that was fed constantly by a buffered aquifer (i.e. the signal averages the occasional storm events, etc.), with a low and steady drip rate (perhaps 1 drop every minute) from a source that does not allow much splash. We want the calcite to have the time to "grow" with minimum lateral loss and as constantly as possible.
Finally, a word about the expectations on climate reconstructions from speleothems. It is true that sometimes one may find speleothems that show annual laminae or seasonal couplets. It is true that we are now able to sample and measure stable isotope values to micrometer scale. However, any climatic interpretation will be hampered by the dating accuracy. So, for a Holocene sample and a 2SD error of +/-1% we can get dates within a +/- 100 yrs error. We must acknowledge this limitation - any stable isotope value we've got can be anywhere within, say, +/-200 yrs. Laminae backcounting and other methods alike are interesting but they cannot account for small hiatuses (e.g. 1-2-3 exceptionally drought years). To put it straight, we cannot ask speleothems more they can do. Let's keep the expectation within the geological timescale where +/-100 years for the Holocene would be absolutely great! 
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These cultured isolates were obtained from a cave. Our experience has shown that cave organisms are difficult, and occasionally impossible to subculture to pure cultures.
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As Olga suggested, you can easily use High throughput sequencing to identify who' s present in your culture. I would suggest performing Multi Locus  Sequencing Technology (MLTS) on NGS to specifically identify the siderophore producers in your culture
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Does some one know if there is any record of precipitation isotope in westerlies controlled region? For example, Xinjiang or Central Asia. There are plenty of records of monsoon precipitation isotope records, such as cave deposits.
The timescale better restricted since LGM or the Holocene. Thanks for your help.
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There has been a recent effort to explore Central Asian caves and retrieve speleothemes to map the regions under predominant westerlies or monsoon influence. I know that Sebastian Breitenbach (ETH Zurich) is working on the analysis of samples from Uzbekistan. You can find some isotope data  (∂18O ∂D) in a conference paper (ICS 2013) he uploaded in the link below.
You might find additional ressource on his profile page: https://www.researchgate.net/profile/Seb_Breitenbach
Good luck