Science topic

Cactaceae - Science topic

The cactus plant family of the order Caryophyllales, subclass Caryophyllidae, class Magnoliopsida. Cacti are succulent perennial plants well adapted to dry regions.
Questions related to Cactaceae
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Greetings!
I´m on a proyect about cacti pollination. My team and I want to learn about pollen techniques to obtain permanent samples, as well with some stain procedure, once extracting it from collected floral visitors.
I´ve readed about acetolysis and another technique using glycerin and hot water.
Any suggestons and experiences are welcome!
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I used light acetolysis, first wash with glacial acetic acid to avoid forming insoluble Cellulose octacetate, and then several water washes, then embed in glycerol or continue drying the growin series of alcohols and then silicon oil of 12,000 centistokes or more viscose if you prefer. both glycerol and silicon oil have pros and cons. My preparations of 1970 with glicerol have expanded a couple of microns; the ones from 1971 in silicon oil have remained mostly unchanged, but preparation is longer and some alcohols such as xylene and benzene are bad for your health and real protection is needed (an efficient fumes hood to begin with). It has been a long while that I don't prepare pollen reference slides. I hope this helps. Best regards
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I want to carried out a study of karyology in cuban cactus and I would like to know advice from experienced people in this field
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For research regarding phytoremediation using cactaceae, specifically gymnocalycium.
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No Cactaceae have not the reputation to hyperaccumulate heavy metals.
Regards
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I know Qiagen makes plant specific extraction kits, but has anyone tried the DNeasy blood and tissue kit with plant material?
Thanks, Matt
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I have not, I use the plant kits. Why do you ask? If you're asking because you can't afford the plant kit, you might look up an old plant protocol and make the reagents yourself. Otherwise I'd suggest you do a side-by-side comparison of blood/tissue versus plant kits.
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Bonjour,
I am looking for informations about the growth speed of Melocactus cephalium.
Would you have data, references ?
seasonnal greetings
marc
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Warm thanks and best seasonnal greetings to all of you who answered
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I 'm trying to find a dichotomous key to cactus (covering all genus) as well as the document where the defining characteristics of the Cactaceae genus are defined. Is there any working group who defines the characteristics to be met by a cactus to be included in a specif genus? any oficial organism?
Thank you in advance
Best regars,
Elena
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Im working with dragonfruit stems under shadow to see his response.
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Mr. Calero,
Apart from the responses of plants to shading which were already mentioned (by Mr. Zervoudakis and Mr. Chhogyel), in addition, shading can cause decreased flowering (general rule) and increased specific leaf area. Regarding the better response of plants to shading, in terms of the appearance and growth of new stems, as you referred, please have in mind that plants adopt their strategy in order to increase their competitive ability under reduced light regimes. The latter may help you to interpret your results. Also, the attached files may be useful to your research.
I wish you quick progress in your research. 
Best Regards,
A. Matsoukis
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I am writing a protocol to determine the biomass of Cactaceae and have hit a road block in finding the allometric equations that determine the biomass of the genus Cylindropuntia. Does anyone have a paper they could send me or a researcher I could ask? Thanks! 
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2. Equation
340.308 * (3.1416 * (CR) * (CR) * (CH))^1.115
Output
Biomass
Reference
Búrquez, A., A. Martínez, S. Núñez, T. Quintero y A. Aparicio. 2010. Aboveground biomass in three Sonoran Desert communities: Variability within and among sites using replicated plot harvesting. Journal of Arid Environments 74 (10): 1240-1247.
Reference Year
2012
 3. Equation
356.983 * (3.1416 * (CR) * (CR) * (CH))^1.416
Output
Biomass
Reference
Búrquez, A., A. Martínez, S. Núñez, T. Quintero y A. Aparicio. 2010. Aboveground biomass in three Sonoran Desert communities: Variability within and among sites using replicated plot harvesting. Journal of Arid Environments 74 (10): 1240-1247.
Reference Year
2011
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I found it in Kåseberga, in the grazing site close to Ales Stenar, Southern Sweden. It looks like a cactus.
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I agree with the identification Onopordum acanthium L.
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I'm trying to do a nucleic acid extraction from cacti. Has anyone worked with that? I'm facing problems to extract DNA and RNA from the aerial part, mainly due to the amount of mucilage. Most of the references about the subject do extract (D/R)NA from fruits, but I need to do the extraction very early after germination.
If any of you have worked with this and have a reference or any suggestion, it will be much appreciated.