Breeding - Science topic

HELLO!

I WORK IN A COMPANY THAT IS WORKS IN AVICULTURE BREEDING, BUT NOW, IT HAS SOME INVESTMENTS IN THE AQUACULTURE FIELD AND I GOT THE CHANCE TO BE A SENIOR PARTNER IN A NEW PROJECT WHICH IS SEABREAM BREEDING, "DORADO FISH" AND THEY ASKED ME TO GATHER INFORMATION ON HOW DOES IT WORKS.

• HOW DO WE GET STARTED AND WHAT ARE THE DIFFICULTIES THAT WE WILL FACE?

• WHO ARE THE COMPANIES THAT WE CAN IMPORT LARVAE AND MATERIALS FROM THEM?

• HOW WE CAN FEED?

THERE IS A LOT OF QUESTIONS, HOPE I GET ANSWERS.

Many farmers still use landraces which give lower yields. For this reason, many are not convinced to cultivate this crop. What are the challenges in breeding for higher yields in this crop? Please help me to unpack this.

I am on the hunt for the following paper that I cannot seem to access online:

Jensen and Jensen 1969. On the Breeding Biology of African Cuckoos. Ostrich 40: 163-181.

Would anyone that has access to this paper be willing to send me a digital copy?

I am looking into ideas for a tank setup & parameters. I am looking to breed for environmental studies.

The selection accuracy in genomic selection (GS) is often calculated as correlation between true genetic value and estimated breeding value. Is it possible to have negative values as selection accuracy? Ideally in a normal correlation, the coefficient ranges from -1 to 1. in GS we expect high selection accuracy, is it not weird to have negative correlation values? What could have responsible for the negative?

Oyster breeding research project

Ladies and Gentlemen

Hello

I am Masi Chakhmaghi from Iran.

since 2018, in order to protect the environment for the future, I started working in the field of mealworm breeding and started a small farm, which I faced many problems.My main problem is the production line of the farm, which has a low yield. Is it possible to help me?

To breed rice for drought tolerance, is it feasible to start selection in the F2 by picking out offspring harbouring certain molecular markers, given that it is expected that these QTLs will continue segregating in the subsequent generations? Or is it better to keep selfing a hybrid population until the F5 or F6 and then start selection?

Generally it is said that indigenous breeds are resistant to different agents leading to various diseases including physiological abnormalities but is it due to some inherent merits like very low production etc and if production will be at par with exotic ones, will they still reveal such resistant or fail or even perform worse than exotic ones which I believe. Has it been proved with detailed research trials/evidence based or simply based on meager reports/findings.

Genetic engineering should be seen as one of the many tools available for use by plant breeders to improve crop varieties so that we increase food production, control pests, and improve farm profits.

ANIMAL BREEDING estimations of breeding values.

Say, I have two topics;

1. the survival of kittens and the parent's breed

2. the government's contribution to service provision

Thank you

Any lab uses a specific breeding scheme (Tg male or female to breed with nonTg parent) to generate Thy1-APPPS1 (Jucker) mice heterozygous pups to investigate amyloidosis? Please let me know!

need to know about the breeding.

Being from the same field of insect breeding, can I have a video of this project?

I am looking for the WGS of a variety of Chickens (Gallus gallus) such as Rode Island Red or White Leghorn. I realize there might not be a great diversity between these breeds but I would like to study these differences. I have already tried NCBI and MGRAST, are there other resources I should consult ?

Just curious question for those of you working on biodiversity and breeding: it generally advised that when we do a PCA, that data be standardized to bring down all the features considered to a common scale without distorting the differences in the range of the values. However, we often have “a feeling” that certain characters (mostly those that can be readily measured or appreciated) are more important than other in distinguishing one genotype from another; i.e. color of the petals of the flower or color of the fruit. Is there a way one can “tilt” the method (the software) into systematically giving more weight to those “decisive” characters? If yes, how one would decide on the weight he/she should give to each independent variable?

I am trying to self pollinate commercial varieties but I don't have the maintenance.

Dear All, I have a data set  from an RNA seq. experiment to compare treated Vs control group in several breeds of animals. In each breed, I want to see how is the extent of the sepration between treatment vs control groups based on 18.000 genes being DE between the two groups. In another word, in which breed was the effect of treatment the greatest ?. Usually one visualize this using MDS plot, but I can not estimate this visually. In my MDS analyses (either the one that run from inside edgeR or the one run on the CPM TMM normalized counts) I could see sepration between treated vs control groups in two breeds.

The question is 1) How can I estimate numerically the divergence of the two groups in each breed so that I can tell the difference in treatment effect in among breeds. I have actually tried calculating the Euclidean distance among control and treated individuals within each breed, but it gave a distance number between each individual within each group in a pairwise manner, so what is the best method to show an overall dissimilarity between control and treatment given the distance between each pairs of individuals of the groups. I also thought about correlation coefficient, but it gave strange results.

I have another question: Are there any relation between the common dispersion (estimated during the DE analyses in EdgeR) among genes in one breed and the number of sig DE genes identified in this breed and the degree of sepration between the two groups in the same breed ? in another word, Is this right that if for instance I found that in breed A the two groups have greater dissimilarity than in breed B, Should I expect higher dispersion for the genes or higher number of sig DE genes in breed A than in B ?

Any comment would be helpful

Hello,

I am using speed breeding for generation advancement in the genomic selection pipeline of durum wheat. in the growth chamber the conditions are the following :

-photoperiod of 22 hours light/2 hours darkness

-humidity 70%

-LED light.

At flowering time I started to see strange spike morphology (as shown in the pictures ). without producing seeds at the end of the cycle ( sometimes 1 or 2 seeds per spike )

Can anyone help me to understand why I am getting this kind of spikes ?

Is there a better method than ultrasound technology that can be relied upon in determining the quality of meat, in order to preserve the breeding values ​​of the ancient Burqi sheep herd?

Greetings to all those interested and eager to help.

In short: During 12 breeding seasons, my colleagues and I researched the nesting of one bird species on an area of ​​about 11,000 hectares. We spent the first two years looking exclusively for territories/nests. We recorded a total of 34 different territories/nests. For the next ten years, we had in mind to monitor the reproductive parameters (laying dates, number of eggs, number of offspring, etc.) for all 34 territorial pairs found. However, due to the vast study area, hard mountain relief, bad weather conditions and lack of time in general, we did not visit every territorial pair every year (we did it completely randomly). So, in some years, we followed the nesting parameters in only five territories, while in others, we managed to monitor up to 20 territories and collect reproductive data. For each year collected data table contained: year, number of controlled nests, number of nests with incubation, number of successful pairs, number of fledglings, productivity and nesting success. We defined productivity as the number of fledged juveniles divided by the number of successful nesting attempts. Nesting success was defined as the number of fledged juveniles divided by the total number of nesting attempts during one calendar year. My question is whether it is possible and in what way (statistical modelling, simple formula, etc.) to express the population trend for the entire monitored population with the help of partially collected reproductive data? So is it possible to project a ten-year overall population trend based on annual productivity and nesting performance data?

Thanks in advance for the comments, suggestions and literature.

Sincerely yours,

Among the cultivated primroses, I observed a plant with 5 stigmas and styles. I could not find a report on this. Does anyone know the reason for this feature? Is there a report on this?

Hi everyone,

there are many studies which prove that the breeding success of raptor populations (or other animals) declines while the breeding densitiv increases. The most common explanation is the habitat heterogeneity hypothesis, saying that additional individuals have to use non optimal habitats when the carrying capacity ot the population is almost reached.

Is there any evidence that individuals from these populations tend to leave their birth areal and search for other habitats in greater distances? Or are raptor populations mainly regulated by their breeding success?

Cheers Tim

Hello all, I am analyzing body mass between small mammals and testing if it varies between sexes, reproductive status (breeding/non-breeding), and season (wet/dry). Would it be alright to have one variable called reproductive class as a factor with 4 variables: reproductive male, reproductive female, non-reproductive male, non-reproductive female. Is it possible to then use this in a linear model like body mass ~ reproductive class * season ?

Does sex and reproductive status have to be separate terms?

Additionally if I have significant differences between males and females, can I group the two sexes together to see if body mass overall changes with season?

Thank you

C4 rice variety development project, involving an international consortium of researchers..

Dear Research,

Here I am finding methodology for pollen processing steps in okra or lady's finger.

suggestions and answers are appreciated.

Thank you

Pollen/ovule ratio is used to estimate breeding system of a plant species. Is that estimation reliable for all plants?

Hi, I want to know which method is most accurate in providing estimates of chlorophyll content.

Can abiotic glycine be a source of organic matter for soils? We want to know the role of glycine in life-forming processes, and to do this, it's worth looking into how glycine affects breeds. We would also appreciate any literature on the subject.

Why are most of the breeding efforts regarding apples involved in breeding apples that are Red or yellow with no russeting? Historically this was not the case and breeding efforts were often in the opposite direction, what caused the shift?

I’m new to the publishing world and I’m hoping to get some advice on the most appropriate model to use to statistically analysing my data please. To summarise the project, I deployed four AudioMoths (which are audio recording devices) at four different sites in the middle of the Davies’ tree frog’s breeding season (one AudioMoth per site). There is virtually no published research on the species and the frog’s daily calling activity has not been described. The aim of the paper is to describe the daily calling activity of the species (i.e., what time of day does the frog call at) and I am hypothesising that the frog will peak in calling activity in the hours just after sunset (i.e., between 7-9 pm).

My AudioMoths recorded 5-mins at the start of every half an hour in a day for a 10 day period. I used an automated sound recognition software called Kaleidoscope Pro to extract the frog’s call from all the audio data. I then calculated how many calls there were in each hour of the day (so there’s two lots of 5 min recordings per hour) and then took the average number of calls per hour from all 10 days for each detector (so each hour has a sample size of 10, 1 hour for each of the 10 days). I then combined the data from all 4 AudioMoths to get an overall average number of calls for each hour of each day (so each hour now has a sample size of 40). I then turned the average number of calls per hour to a percentage of calls per hour and created the attached graph with standard error bars.

What I’m hoping to get help with is how to appropriately test if there is a difference between the number of calls in each of the 24 hours per day? Would I use an ANOVA initially to see if there is a difference in the number of calls between any of the hours and then follow it up with a post-hoc test to find out where the differences are? Or would it be best to compare the percentage of calls per hour and use a chi-squared test? Or is there another better option? What problems arise when I’m comparing 24 different groups (because there are 24 hours in a day)? I’m mainly wanting to know how best to show statistically that there is a peak in calling between 7-9pm (if there is in fact a statistical peak)? If there is a statistically significant peak in calling between 7-9pm, then future field surveys can survey in this time period to maximise the probability of detecting the species.

I’d love to hear your thoughts on this if you have the time please! I’d also really appreciate to hear your explanation as to why you suggest the approach you do please?

Now a days, speed breeding is beIng used to advance segregating generations by taking 4-6 generations in a year generally to develop RILs and other mapping population. should a breeder use this technique for varietal development program or not ? Why

We all are facing the problem of increasing climatic change and abiotic stress is increasing day by day and organic agriculture is getting challenged so how the science community will face this all.

I wonder is there any information about the mutation in intron regions related to phenotype.

Which new/modern softwares are being used for SSR analysis ? i mean for genetic diversity, to estimate inbreeding or out breeding guess of population in plants

We are working with white-winged snowfinches, alpine bird species breeding in rock crevices, roofs and skilift pylons and struggle to access nests in some of the deeper cavities (50 cm to 1 m). We are using an endoscope, but it is often difficult to access nests in deep cavities when they are really contorted (as we dont know the internal structure of the cavities).

We are mainly interested in counting the number of chicks, but also to place ibuttons for temperature measurements if someone has an idea how to place (and retreat!) them.

I am interested to know which methods people working on similar cavity-breeding birds (preferably rock crevices, as we are facing unique problems ina ccessability and cavity structure as f.i. woodpeckers will not have) use to gather nest information (if at all?).

Thank you very much in advance, Christian

Do you know any successful example of Hologenomics as a breeding approach for beneficial microbial traits incorporation in any crop plants???

We currently have three breeding strategies going. We have the following strategies:

A-strategy: Triple transgenic mice are being backcrossed onto a C57BL/6 background to produce mice for breeding

B-strategy: Single transgenic mice are being expanded for breeding with A-mice (C57BL/6 background)

C-strategy: A-mice (male only) X B-mice (female only) are being bred.

Two triple transgenic female mice (in A-strategy) have died either while giving birth or about to and two female B-mice (in C-strategy) have died either while giving birth or about to.

Other breeding going on in our facility have been normal and okay, with no deaths. The lab we received the mice from have not reported problems like this.

Literature primarily focuses on pups dying and we have now moved the mice to the room where there is least human traffic to minimise stress.

Any help or advice would be greatly appreciated!

Does it help for work to discover the potential capabilities of native breeds and follow selection programs for their genetic traits effectively in facing the current challenges of climate change?

I am designing an experiment using same buck on different numbers of does but at different times. What minimum period should be rested?

The availability of relatively large number of disease resistant varieties compared to insect resistant cultivars suggest that incorporating disease resistance is relatively easy. This is also evident from the list of registered donors and available literature. Should it be understood that breeding for disease resistance is easier than that for insect resistance?

If a breed is under risk of being extinction and it is being maintained in an organised farm (Effective population size till now manitained) but its revealed inbreeding depression in term of some functional traits. No other population of this breed are available in their breeding tract.

Organizations working

Methodology

Example: rearing in breeding cages or ground cages?

I want to know if anyone has any experience regarding roles of Kisspeptins in regulating the onset of puberty, and possible role in enhancing conception in heifers during the first breeding season

We have been conducting one continuous camera trap survey (2019 - present) with (n=40) camera traps set up across our study site. The objective is to determine prey availability in terms of demographic classes. However, since the majority of the prey species are not identifiable to individual, we are limited to unmarked species. Furthermore, we are open to the idea of using models that require population closure but will have to violate the assumption as we are purposefully comparing prey availability between breeding and non-breeding seasons.

Thank you in advance.

Dear,

I need publications to discuss the "adaptation" of native (local) animal breeds to harsh environmental conditions. In a different aspect than usual. In particular, under poor feeding conditions when cows shorten lactation and not (or slightly) lose body weight. Which is the opposite to Holstein-Friesian and other high-yielding breeds. I know this is an archaic topic. I have some publications about adaptation of African breeds but there it rather concerns heat stress. Since it can be difficult to discuss about cattle I will be satisfied with other ruminant species as well.

Is the determination of LC50 of any compound in Zebra fish during the breeding season cause any problems?

Hello,

we are searching for publications on the occurrence of European nightjar (Caprimulgus europaeus) in solar parcs. Does the species breed and/or feed there? How do the photovoltaic modules affect the occurrence of the species?

Thank you for your replies.

Hello I am looking for papers regarding the history of the breeding programs of haskap (Lonicera caerulea) in Russia and Japan. I have been having trouble finding such papers. I would be quite grateful for any information that can be provided.

Thank you.

What is the current status of biofortification programs in chickpea. Recent progress in this research area. How many percentage of chickpeas are biofortified with agronomic, transgenic and breeding methods?

FUHML(mm) FS(cN/tex) MV FU(%) SF(%) FE(%)

The marshes are tremendously important because of the essential services they provide, such as : habitat for wildlife, fishes , turtles and invertebrates species . Nutrients in the marsh support an abundance of plant and animal life, including serving as the breeding  ground for economically important fishes we eat.

Is this use molecular weight or binary data for this software. I was using molecular weight and got error 32.

I'm looking for some standard literature on Asteraceae pollination and methods to conduct breeding system experiments on Asteraceae flowers.

The new study provides insights on how the new strains of COVID may have originated. All three of the new strains have changes to their spike protein which make them more transmissible, deadly, or immune-resistant.

if the initial body weight of chicks differs significantly between two breeds of broiler , so should we apply covariance to compare the effect of breeds on final body weight ?

I need a program to detect mutation in DNA and compare results from different breeds or species.

Thanks in Advance

Maize (Zea mays L.), a crop with very much of nutritional & dietary importance doesn't have good yield & productivity(2.84t/ha in Nepal & 5.82t/ha in world in 2019 ) (FAOSTAT, 2021)as the plant is tall & do not bears any tillers. And the demand of Maize cereals as food & concentrate will increase a geometrical ration on future. The study on breeding to make Maize; a tillering type of cereal crop had not been heard by me. So, I want to ask what are the research the aforementioned subject?

A question on mouse crossing: Do you think it is possible to breed two genetic mutations (6kb apart) at a homologous locus onto a same chromosome, or in cis? Because the crossover between two mutations may require meiosis recombination, I thus plan to interbreed heterozygous A-b/a-B littermates, and genotyping all pups with long range PCR. Do you think this is a feasible plan? How many breeding pairs / scale would you recommend? Thanks!

One of the challenges in onion breeding under tropical condition is flowering. Our first step is to develop onion lines capable of flowering all throughout the year.

At University, we are doing an assignment on the development of certain behaviours from wolves to dogs. My friend made an excellent point. When wolves were domesticated, they were bred to remove aggression and to increase tameness. But when people now breed dogs for aggression, then they lose control over their dogs, lose the tameness (to an extent) there fore are almost "undoing" domestication with their dog.

I just wondered if there were any studies that have looked into this, or if anyone had any thoughs on the subject.

I am planning to collect popular local maize varieties and carry out conventional maize breeding (bulking method). I don't have a clear idea about it.

I am looking for a protocol which is simple but effective in getting highest number of embryos after uterine flushing

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that were then crossed. For future planning, is it reasonable to expand a Cas9 KI+/+ Cre+/+ colony and then breed these animals to a Cas9 KI+/+ Cre-/- mice? Then all pups will be Cas9+/+ Cre-/+. The experiment require a large quantity of male mice.

I cordially invite researchers to contribute an MS on “Value Addition in Biofuel Production Using Innovative Breeding Techniques” for the forthcoming edited book entitled “Technologies in Breeding Field Crops” (Springer Publication). Kindly send your email id for a formal invitation.

Now a days, speed breeding is becoming famous for rapid varietal development through rapid generation advancement.

However, it's applicability and feasibility for everyone and everywhere is still not wider. What you think about it? How will it be useful in future breeding for crop improvement.?

Animal breeding is all about big data management and handling. The big data management is not possiable without application of computer in animal breeding. As data analysis and interpretation has pivotalrole in animal breeding. Hence, to have insight about the softwares used by animal breeders for data handling and analysis is necessory for a breeder is very important process.

Traditional breeding methods are one of the most important methods for obtaining new varieties of sesame. However, it takes a long time. Are there ways to reduce the time required to obtain a variety of sesame?

Hello friends Good time. I need some issues with the genetically modified goat breeds(composite or synthesis) in the world. Please help me.

I have cultured chital on live feed of 2-3 GM with a SD of 5000 in 0.7 ha. after 2 month culture the total weight is approx 15-17 GM...and tilapia of 250-350 GM....now we are assuming that tilapia is breeding in the chital pond and the tilapia young one is fed by chital...but recently we are getting few species of chital dying due to unidentified reason. please help me figur it out to resolve the issue...pics are ATTACHED

Why animals are (cow, dog, goat etc.) matting only before breeding?

I am doing semantic analysis of science and grey literature publications in the field of animal genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art terminology in chicken, goat, sheep, cattle genetics and breeding research. Can you advise reference publications/standards on characterizing animal genetics research and development activities, and the outputs it generates?