Bone Regeneration - Science topic
Bone Regeneration is a renewal or repair of lost bone tissue. It excludes BONY CALLUS formed after BONE FRACTURES but not yet replaced by hard bone.
Questions related to Bone Regeneration
Where is information about the solubility of bioactive glass 45c5 and apatite-wollastonite? Why can't it be found?
Where is information about which calcium phosphate compounds are used and for what purpose? Thank you!
Which works contain information about the mechanical as well as biological properties of the cerabone AW biomaterial, namely biodegradation. bioactivity, biocompatibility, osteoconduction, osteoinduction? Thank you!
It is necessary to select biomaterials (hydroxyapatite, 45c5 bioglass and A/W bioglass) using a hierarchical method, but I cannot find indicators of biocompatibility, biodegradation, osteoconductivity of these materials. What studies or standards have information about these indicators of these materials? Thank you!
Want to design a nanocomposite-hydrogel containing growth factors and drug molecule which enhance bone regeneration. But this hydrogel will not contain stem cells.
Can this hydrogel be used as a scaffold to regenerate bone tissue in an affected area?
Are stem cells essential to make scaffold for bone regeneration?
If anyone knows the process, please help me to understand.
I am about to synthesis and characterize a bone scaffold to be used for bone regeneration and bone cancer therapy with the ability to release drug and oxygen for a better functionality, which structure can be more effective for my system, a hydrogel scaffold or a 3D printed or f-dried scaffold?
I prepared 3D porous composite scaffold (nano hydroxyapatite-PLGA) for bone regeneration and seed it with cells. First i checked the cell viability, now i want examine the cells inside the scaffold and stain them. I tried to embedde my scaffold in parafin wax but during sectioning the scaffold came out crushed & powdery. Is there is any way to section my seeded scaffolds ? Or i need to try cryosectioning, it may give better sections? Any recommendations..
In response to this question, I can only find experience based recommendations in the literature that differed from as early as 3 weeks to as late as 12 weeks. There is a single animal experiment from Japan (Uji, 1996) in cats that compare one week and 8 weeks, but the experimental model does not really mimic that of a cleft. Recently, a couple of studies addressed the issue of moving teeth into the post distraction bone regenerate which may be considered quite similar. Does anybody know any research-based recommendations done on cleft material?
i read in a book that metaloproteinases secreted fom osteoblasts are required in bone resorption to remove osteoid
Copper and Cobalt can improve osteogenic differentiation of mesenchymal cells, but can it be somehow connected with hyaluronic acid?
According to WHO, "the categories of xenotransplantation procedures include the following:
Solid organ xenotransplantation; cell and tissue xenotransplantation; extracorporeal perfusion ; exposure to living animal-derived material "
What resources or articles would you recommend for their procedures?
Dear professors, I have a question about rare earth elements in the bone repair process. As we all know, rare earth elements like lanthanides are toxic, but I have seen some reports that low concentrations of rare earth elements will promote it to a certain extent The differentiation of osteoblasts and the inhibition of the reproduction of osteoclasts. Is there a certain basis for this statement? Can experiments in this area be tried? Or that rare earth elements are heavy metals and are not beneficial to bone regeneration.
If the professor knows the answer, I hope you can answer it for me. Thank you very much!
Space availability is less and miniature sized expanders are required
We need this cell line for some of our experiments related to bone regeneration urgently. If somebody can give us a stock then that will be very useful. Thanks
In my research, we want to fabricate scaffold of collagen type I and nano-hydroxyapatite for bone regeneration. For that, we want to extract collagen type I from pig skin (the tissue might be changed). From reviews I've learned that salting out is the best way to extract collagen type I for it's not require hydrolysis.
Does salting out is the best way? If you have any protocol for extraction, please send me. I'll be thankful for insights and tips for the procedure.
Both may appear radiopaque
Can you help to solve a problem?
What compounds should we prescribe locally to induce a better osseointegration with implants?
Biphosphonates and BMPx (as a GF) are good candidates for a local use, but may be there are some new compounds that can enhance bone formation? Novel studies suggest dlk1 protein. Most likely it would be an expensive process.
Would be useful to use local antibiotic and anti-inflammatory drugs as well to decrease an imflammatory response and to reduce an external tissue damage?
Thank you for your help!
I understand what water swelling behavior means, but how is that related with a materials properties to assist in bone regeneration ?
I need to perform the aforementioned analysis in one of my projects, but I don't have too much experience in doing that. Where could I find a tutorial on the topic?
Better yet, should anyone know an experienced pathologist that could perform this analysis, I would be willing to hire his/her services.
What are the most popular bone biomarker proteins for bone regeneration please? thanks in advance.
Hi Can I test bone regeneration in vitro using cells other than bone stem cells? for example, using cancer cell line and testing bone biomarkers? thanks
I am now doing a study that relate to bone regeneration. I want to ensure the procedure for the ALP assay which are faster and cheaper. I did review some previous study on ALP Assay. Some of study are using Kit for the assay which is easier, however that price for the Kit is quite expensive. Meanwhile , the other study also shows ALP assay by doing it manually. I want think to do it manually, but i am afraid there will be a step that i will misconduct during the experiment. So, here i want to know which of the method is preferable for me to proceed the test. Is anyone are familiar with ALP assay for bone regeneration (osteoblast).
Does telescopic transphyseal nailing like Fassier-Duval prevent secondary torsion deformities of femur or tibia in children with osteogenesis imperfecta during residual growth? I am especially interested in cases after deformity correction osteotomies. Thank you.
I want to evaluate the two aforementioned parameters (bone regeneration and angiogenesis) in rabbits, using DCE-MRI, histology and micro-CT. I have taken a look at the literature, but there doesn't seem to be a consensus. Therefore, using a few papers as references, I've decided to evaluate these parameters at 2, 3, 4 and 6 weeks after surgery. Are these time-points adequate?
I'm working on a project about tibia's fractures and I need to know the mechanical proprieties (Elastic Modulus, Poisson's Ratio, Mass Density, Yield Strength) of the Callus Bone (Regenerated Bone). Thanks
I intend to evaluate bone regeneration and angiogenesis in rabbits undergoing bone grafting with different materials. I look into the literature and I've found studies using 1, 2, 4 defects. I would like to use four 8mm defects, but I'm afraid that they might be too close to one another. Could that interfere on healing? Should I use less defects?
I'm working on an animal experiment to evaluate whether DCE-MRI can be used to monitor angiogenesis during bone regeneration. For that, I'll use 16 rabbits, which will be randomly divided into 4 groups of 4, according to the time of euthanasia. I'll create four bone defects in the rabbits' calvaria and fill them with two experimental materials (Bio-Oss and BoneCeramic), a positive (autogenous bone), and a negative (blood clot) control. Two weeks after surgery, a group of 4 rabbits will be submitted to DCE-MRI and then sacrificed for ex-vivo micro-CT and histological analysis (current gold standards, to which I will compare DCE-MRI). After 3 weeks, another group of 4 rabbits will undergo the same procedure, another group at 4 weeks and finally the last group at six weeks. Animals will be anesthetized with a combination of ketamine/xylazine for surgery and with a combination of fentanyl/propofol for imaging.
Do you use platelet lysate injection to accelerate bone consolidation during distraction osteogenesis?? if yes how many times??
Dear friends, please help me to find a reference to the healing of the bone tissue of the skull. Dates trepanation healing I need. Thank you!
I would ask if anyone can explain me how and if there is amodifciation in bone density due to repetitive microtrauma and after fracture.
If these alteration exist, is it possible to clinically palpate it?
Thanks for the answer
Autologous bone grafts
I want model bone remodeling by titanium nano tubes in comsol multiphysics software, and I need to model osseointegration between bone regeneration and Implant contains TiO2 nano tubes.
What is required boundary conditions?
Bone regeneration between cell and material.
Various bone graft and alloplastic materials are available on the market. It is very difficult to select the best one for regenerative osseous surgery. Since we cannot do histopathology for confirmation of bone regeneration. Same as to select GTR membrane. Some absorbable membrane are dissolved very rapidly too.
I am using injectable gel system for my studies. Will there be any soft tissue in-growth in the defect area?
Acemannan is an extract from Aloe vera
Over the past three decades great strides have been made in the field of periodontal regeneration. Reviews of the literature identify many surgical techniques and materials that have been used successfully to obtain new clinical and histological attachment. Although to date the goal of complete, predictable regeneration has not been attained, the literature has clearly demonstrated the clinical feasibility and histological possibility of periodontal regeneration with many of the procedures. But now the question arises: are those true?
I am looking for some MATLAB or Python codes for bone remodeling based on stresses due to daily activities. I came across many papers where people have used these in their study. Is there anyone who has shared these codes on free domain or are open access?
We are in the fourth stage of our investigation and the results look very promising.
Bisphosphonates and PTH increase bone formation, but they don't seem to be capable to control bone resorption.
Which one will be provide more reliable results and minimal errors during procedure?
I need to perform either of these to show there is increased protein/mRNA expression because of scaffold which I incorporated in rat skull bone.
Which stain demonstrates new bone and ostoid in Technovit 7200 resin ground and polished sections?
There are several time-groups (4 weeks, 8 weeks, 6 months, 9 months) and several alloys. Which IHC-marker should be most informative to study and compare in these groups? Especially in 6 and 9 months?
After a tooth extraction, an implant was used after 6 months but it does not work because of a failure in bone healing. The implant was removed. I would like to use HBOT to improve the bone healing before using a new implant. Few articles are published on this subject but some of them suggest a positive effect of HBOT.