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Blotting Techniques - Science method

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Publications related to Blotting Techniques (710)
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Article
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Biofilm infection has been identified as a crucial factor of the pathogenesis of chronic wound, but wound biofilm diagnosis remains as an unmet clinical need. We previously proposed a modified wound blotting technique using Alcian blue staining for biofilm detection that was characterized as being non-invasive, time-saving, non-expansive, and infor...
Article
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Background Hydatid cyst (HC) is the larval stage of the canine intestinal tapeworm (cestode), Echinococcus granulosus. In addition to the high global economic cost to livestock farming, the infection can lead to dangerous problems for human health. Therefore, research into new diagnosis and treatment approaches is valuable. This study is set out to...
Article
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Mesenchymal stem cells (MSCs) are multipotent cells found in various tissues and are easily cultivated. For use in clinical protocols, MSCs must be expanded to obtain an adequate number of cells, but a senescence state may be instituted after some passages, reducing their replicative potential. In this study, we report a case where MSC derived from...
Article
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Background Flower color is one of the main characteristics of ornamental plants. Aurones are light yellow flavonoids produced in the petals of a limited number of plant species including snapdragon ( Antirrhinum majus ). As a commercially-recognized species, African violet can be found in various colors except yellow. This research, aiming at chang...
Article
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Background: Facioscapulohumeral muscular dystrophy is the third most commonly found type of muscular dystrophy. The aim of this study was to correlate the D4Z4 repeat array fragment size to the orofacial muscle weakening exhibited in a group of patients with a genetically supported diagnosis of FSHD. Methods: Molecular genetic analysis was perfo...
Article
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Obesity is a risk factor associated with age-related disorders that accelerate aging, and it increases the risk of metabolic diseases. Therefore, this study was conducted to investigate the association of leukocyte telomere length (LTL) with the presence of higher body weight in middle-aged females and males. The study subjects comprised 160 (80 co...
Article
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Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection. This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology. Method Lectin microarray containing 56 lectins was used t...
Article
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Leptospirosis a public health problem and an endemic zoonosis in Brazil, is diagnosed by serological methods. Therefore, low-cost and easy to execute methodologies with good/high sensitivity, such as Dot Blot, are an important diagnostic tool. The aim of this study was to standardize and validation the dot-blot technique for the serological diagnos...
Article
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Background Recent developments in abscopal effect strongly support the use of radiotherapy for the treatment of metastatic disease. However, deeper understanding of the molecular mechanisms underlying the abscopal effect are required to best benefit a larger proportion of patients with metastasis. Several groups including ours, reported the involve...
Article
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Tomato (Solanum lycopersicum L.) yield is severely affected by Fusarium fungal disease. To improve the resistance of tomato against Fusarium oxysporum f. sp. lycopersici (FOL), Escherichia coli katE gene was transformed into two tomato cultivars, namely Castle Rock and Super strain B, via Agrobacterium tumefaciens; the transformation efficiency was...
Preprint
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Background. Recent developments in abscopal effect strongly support the use of radiotherapy for the treatment of metastatic disease. However, deeper understanding of the molecular mechanisms underlying the abscopal effect are required to best benefit a larger proportion of patients with metastasis. Several groups including ours, reported the involv...
Article
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Buffalo calves have a high adverse effect on public health, specifically zoonotic Giardiasis in Egypt. The current study was carried out to investigate the use of enzyme-linked immunoelectrotransfer blot technique (EITB) as a preliminary step for the diagnosis of giardiasis before genotyping. For this purpose, fecal and blood samples were collected...
Preprint
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Rapid modifications have been done to solve issues regarding conventional western blotting(WB). Advanced kits and instrumentations introduced by multiple biotech companies have automated, programmed and made blotting protocols more accurate in a ten-year period. Microfluidic system and capillary electrophoresis have made western blotting much non-c...
Article
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B-biotype whitefly Bemisia tabaci (Gennadius) reported for first time in pumpkin fields of northern parts of Karnataka by using symptomatic and biochemical diagnosis asays such as silverleaf disorder and filter paper dot blot techniques which can able to distinguish infestation of B biotype white-fly in plants from Indian indigenous biotypes easily...
Article
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Background Haptoglobin (Hp), an acute-phase protein, is known as a potential diagnostic biomarker in human diseases. Two alleles of Hp (Hp1 and Hp2) exist in humans allowing three phenotypes (Hp1-1, Hp2-1, and Hp2-2), which influence the biophysical and biological properties of Hp. Objective This work aimed to investigate the variation of serum le...
Article
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In the recent times, we have witnessed some of the major outbreaks of various diseases in human beings and animals and the ultimate conclusion drawn is, the traditional diagnostic tools and techniques are not up to the mark at all particularly for a rapid diagnosis. Hence, there is a need of dynamic techniques with a wide spectrum of applicatio...
Article
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The use of enzyme linked immunosorbent assay (ELISA) for the detection of plant viruses is well documented. It proved to be a very valuable detection tools for the plant viruses. The efficiency of the ELISA technique was for practical purpose independent of the ratio of antibodies to antigen. This avoids the necessity of making specific enzyme conj...
Article
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Increasing evidence indicates that chemotherapy results in long-term effects on cognitive dysfunction in some cancer survivors. While many studies have established the domains of cognition and corresponding regions in the brain most affected, little is revealed about the potential molecular mechanisms that mediate these adverse changes after treatm...
Article
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Oxidative modification of proteins in photosystem II (PSII) exposed to high light has been studied for a few decades, but the characterization of protein radicals formed by protein oxidation is largely unknown. Protein oxidation is induced by the direct reaction of proteins with reactive oxygen species known to form highly reactive protein radicals...
Article
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Human Papillomavirus Genotyping among Different Cervical Smears in Duhok/Iraq Abstract Background/Objective: Cervical cancer ranks the second among the most common gynecologic cancers. This study was established to determine the distribution of cervical HPV genotypes among different Pap readings in Duhok/Iraq. Methods: Between January and September...
Article
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Human Papillomavirus Genotyping among Different Cervical Smears in Duhok/Iraq. Asian Pac J Cancer Prev. 2019;20 (7):2059-2064. DOI:10.31557/APJCP.2019.20.7.2059 Intisar S. Pity1, Hanaa M. Abdo2, Amer A. Goreal3 ABSTRACT Objectives: This study was established to determine the distribution of cervical HPV genotypes among different Pap readings in Duh...
Conference Paper
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Myoglobin is a hemoprotein with the important role of being the binding and storage agent of oxygen in tissues. That is why it is important to study the existence of myoglobin in hypoxia-tolerant animals, such as the green turtle (Chelonia mydas). This study aims to detect myoglobin protein in green turtle organs which consume highly concentrated O...
Article
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Background/objective: Cervical cancer ranks the second among the most common gynecologic cancers. This study was established to determine the distribution of cervical HPV genotypes among different Pap readings in Duhok/Iraq. Methods: Between January and September-2016, HPV-DNA was tested in 64 women. Genotyping was carried out by the hybridization...
Article
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Fibrinolytic enzyme gene (fibE) is widely conserved among Bacillus spp. belonging to group I species. This is encoding a serine-like enzyme (FibE) secreted in extracellular medium. This present work aims to assess the molecular usefulness of this novel conserved housekeeping gene among group I Bacillus spp. to identify and discriminate some related...
Article
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Hydatidosis has been recognized as one of the most important zoonotic diseases in different parts of the world, including Iran. Serological methods that can detect hydatidosis with high sensitivity and specificity are have high value. In this study, the antigen B fluid of hydatid cysts was isolated and Dot-Blot technique performed on serum samples...
Article
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Infectious coryza (IC) is nearly a curse for poultry farmers, when occurs concurrently with several pathogens causing swollen head syndrome. The disease is caused by Avibacterium paragallinarum, which inflicts an initial damage to the nasal and respiratory epithelium. This facilitates the progression of disease pathology across the nasal cavity, th...
Article
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Nerve growth factor (NGF) is a neurotrophic factor that is functional in the survival, maintenance and differentiation of nervous system cells. This protein has three subunits, of which the beta subunit has the main activity. Its structure consists of a cysteine knot motif made up of beta strands linked by disulfide bonds. It can be used as a thera...
Cover Page
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TABLE OF CONTENTS Section 1: Protocol for Flow Cytometry Section 2: Protocol for DNA isolation/extraction with CTAB method Section 3: Polymerase Chain Reaction Analysis/protocol Section 4: Writing PCR Programme Section 5: Protocol for Southern Blotting Techniques Section 6: Protocol for Total RNA Isolation Section 7: Protocol for Protein Extracti...
Article
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Introduction: In carious teeth, transforming growth factor beta 1 (TGF-β1) is released from the dentin matrix and possibly activated in an acidic environment. Conversely, EDTA solutions with a neutral to slightly alkaline pH are used in clinics to promote cell homing in regenerative endodontic procedures. We hypothesized that citric acid (CA) might...
Article
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Background: Allergic sensitisation towards cashew nut often happens without a clear history of eating cashew nut. IgE cross-reactivity between cashew and pistachio nut is well described; however, the ability of cashew nut-specific IgE to cross-react to common tree nut species and other Anacardiaceae, like mango, pink peppercorn, or sumac is largel...
Article
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Development of a dot blot technique for detection of vitellogenin in sea bass (Lates calcarifer) for using as a biomarker of endocrine disrupting chemicals (EDCs) exposure to the environment. Dot blot technique by using a specific monoclonal antibody to vitellogenin of sea bass (MAb-sea bass VTG F3-23) can be measured vitellogenin levels at 7.8 ng....
Article
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Ischemia-reperfusion (I/R)-related disorders, such as stroke, myocardial infarction, and peripheral vascular disease, are among the most frequent causes of disease and death. Tissue injury or death may result from the initial ischemic insult, primarily determined by the magnitude and duration of the interruption in blood supply and then by the subs...
Article
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Northern blot analysis detects RNA molecules immobilized on nylon membranes through hybridization with radioactive 32P-labeled DNA or RNA oligonucleotide probes. Alternatively, nonradioactive Northern blot relies on chemiluminescent reactions triggered by horseradish peroxidase (HRP) conjugated probes. The use of regulated radioactive material and...
Article
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Introduction: Accurate identification of the peripheral margin of cutaneous basal cell and squamous cell carcinomas plays a crucial role in ensuring complete excision of the skin cancer. The recommended margin of excision for cutaneous malignancies varies in the current guidelines. The aim of this study was to assess the success rate of peripheral...
Preprint
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We aimed to evaluate a multiparametric test system for diagnosing autoimmune liver diseases in a Bulgarian cohort of patients. Methods: We investigated serum samples of 67 consecutive patients: twenty with autoimmune liver diseases (autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), and primary sclerosing cholangitis (PSC), fourty-seven w...
Article
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Negative stain electron microscopy (EM) allows relatively simple and quick observation of macromolecules and macromolecular complexes through the use of contrast enhancing stain reagent. Although limited in resolution to a maximum of ~18 - 20 Å, negative stain EM is useful for a variety of biological problems and also provides a rapid means of asse...
Article
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HPV infection is known to cause cervical cancer. This study aimed to identify the variant of HPV genotypes of cervical precancerous lesions from low grade squamous intraepithelial lesion (LSIL) and high grade squamous intraepithelial lesion (HSIL). This was an explorative study using formalin fix paraffin embedded (FFPE) from cervical precancerous...
Article
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Background: Heat-labile (LT) and heat-stable (ST) toxin variants of enterotoxigenic Escherichia coli (ETEC) are enterotoxins associated with diarrhea among children in Abuja, Nigeria. Enterotoxigenic Escherichia coli is also known as a major etiological agent of diarrheal disease among travelers in developing countries. Continuous identification of...
Article
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Background: Canola is an agro-economically oilseed crop. Yield loss due to fungal disease of stem rot caused by Sclerotinia sclerotiorum is a serious problem in canola cultivation. Thaumatin-like proteins are large groups of the pathogenesis-related proteins which provide resistance to the fungal infection in response to invading pathogens and play...
Article
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Objectives: To explore the independent associations of sedentary behavior and physical activity with telomere length among Chinese adults. Methods: Data on total time of sedentary behavior, screen-based sedentary behavior (including television watching and computer or phone use), moderate to vigorous physical activity, and dietary intake of 518...
Article
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Arabinogalactan proteins (AGPs) are a highly diverse family of glycoproteins that are commonly found in most plant species. However, little is known about the physiological and molecular mechanisms of their function. AGPs are involved in different biological processes such as cell differentiation, cell expansion, tissue development and somatic embr...
Article
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The channel catfish virus (CCV) is a pathogenic herpesvirus that infects channel catfish Ictalurus punctatus in pond aquaculture in the southeastern USA. Mannose-binding lectin (MBL), an innate immune protein, could play an important role in the innate response of channel catfish by binding to CCV. Cell cultures of CCV were grown in channel catfish...
Article
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248 248 Bionatura • Volumen 2 / Número 1 • http://www.revistabionatura.com INVESTIGACIÓN Identidad de la vacuna contra Streptococcus pneumoniae " Quimi-Vio " mediante la técnica Dot Blot empleando los anticuerpos monoclonales contra los polisacáridos capsula-res 1,5, 6B, 14 y 19F de la bacteria. Identity of the " Quimi-Vio " Streptococcus pneumonia...
Article
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The present study was initiated to quantify the Isoflavones content that accumulated in soybean transgenics with RnPPARγ gene by using high performance liquid chromatography. The effects of methanolic leaves extract of transformed soybean in 3T3-L 1 adipocyte cell line. The RnPPARγ transgenic soybean variety JS335 was developed and transgenics were...
Article
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The present study was initiated to quantify the Isoflavones content that accumulated in soybean transgenics with RnPPARγ gene by using high performance liquid chromatography. The effects of methanolic leaves extract of transformed soybean in 3T3-L 1 adipocyte cell line. The RnPPARγ transgenic soybean variety JS335 was developed and transgenics were...
Poster
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Cotton leaf curl disease (CLCuD) is the main cause of the decline of cotton production across the globe, especially in India, Pakistan, and China. CLCuD is caused by five distinct species of cotton leaf curl virus (CLCuV). Two unique alpha and beta satellite molecules are associated with the main genome of CLCuV and are responsible for the disease...
Article
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Bispecific antibodies (BsAbs), with the ability to recognize two different epitopes simultaneously, offer remarkable advantages in bioassays, cancer therapy, biosensors, and enzyme electrodes. Preparation and purification of BsAbs in adequate quantities remains a major hurdle in their use in various applications. Poor yield is also the principal li...
Article
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N⁶-Methyladenosine (m⁶A) modifications of RNA are diverse and ubiquitous amongst eukaryotes. They occur in mRNA, rRNA, tRNA, and microRNA. Recent studies have revealed that these reversible RNA modifications affect RNA splicing, translation, degradation, and localization. Multiple physiological processes, like circadian rhythms, stem cell pluripote...
Article
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In the past two decades, DNA techniques have been increasingly used in the laboratory diagnosis of tuberculosis (TB). The (sub) species of the Mycobacterium tuberculosis complex are usually identified using reverse line blot techniques. The resistance is predicted by the detection of mutations in genes associated with resistance. Nevertheless, all...
Article
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The straightforward assessment of fluorescent compound interactions with proteins in living cells is an important issue in biomedical research, clinical diagnostics and drug development. In-gel detection of compound-protein–bound complexes is often impossible due to the extinguishing of fluorescence in aqueous environments. This study demonstrates...
Article
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The article summarizes over 20 years of experience of a reference lab in fragile X mental retardation 1 gene (FMR1) molecular analysis in the molecular diagnosis of fragile X spectrum disorders. This includes fragile X syndrome (FXS), fragile X-associated primary ovarian insufficiency (FXPOI) and fragile X-associated tremor/ataxia syndrome (FXTAS),...
Conference Paper
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Thalassemia is a disorder in the globin chain as a result , the hemoglobolin. Leads to reduced a ability to carry oxygen , anemia , splenomegaly,Bone deformity and the detection techniques for couples before marriage is. In the present study ,40 of 130 patients with Thalassemia that were Medical cooperate in this research were selected as, was used...
Book
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This book covers the undergraduate and postgraduate courses in genetics prescribed by the Veterinary Council of India. This book would be useful to the students and teachers of Animal Genetics and Breeding. This will also be useful for those preparing for competitive examinations such as Agricultural Research Services (ARS) examination of the India...
Article
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A major problem in forest clonal productivity is the loss of morphogenetic capability with the increasing age of plants. However, despite of the importance of loss of morphogenetic competence, very little research has been done about the underlying mechanisms involved in this process. For this reason, a gene expression analysis using dot blot techn...
Article
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Background & objectives: The amount of foetal haemoglobin that persists in adulthood affects the clinical severity of haemoglobinopathies including β-thalassaemia major and sickle cell anaemia (SCA). The present study was undertaken to analyse β-thalassaemia as well as SCA patients for the single nucleotide polymorphism (SNP), rs11886868 (T/C) in B...
Article
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A major problem in forest clonal productivity is the loss of morphogenetic capability with the increasing age of plants. However, despite of the importance of loss of morphogenetic competence, very little research has been done about the underlying mechanisms involved in this process. For this reason, a gene expression analysis using dot blot techn...
Article
Full-text available
A major problem in forest clonal productivity is the loss of morphogenetic capability with the increasing age of plants. However, despite of the importance of loss of morphogenetic competence, very little research has been done about the underlying mechanisms involved in this process. For this reason, a gene expression analysis using dot blot techn...