Blood Glucose - Science topic

T2D is quite a complex scenario with up- and down-regulation of cytokine expressions. In case of poor controlled T2D (contribution of hyperglycemia/high blood glucose and glycated serum proteins/AGEs, which anti-inflammatory biomarkers exhibit a downregulated expression?

The company Hela Glucose (Movano) is developing a ring-shaped product that promises to measure blood glucose using spectroscopy. The question is whether this would be the most accurate and non-invasive way to measure blood glucose, and if not, what would be the most accurate and non-invasive method?

After lapse of a few months, we recently attempted insulin-tolerance tests in C57BL/6N female mice at 6 months of age. We began, years ago, with Humulin-r at 0.75 U/kg. In our recent tests we used the same protocol, but the only response was a slight increase in blood glucose! We tried several higher doses. At 1.75 U/kg a very slight decrease was observed, but not until 60 minutes. We purchased a non-clinical preparation of insulin from a company that sells a lot of growth factors and cytokines. It produced the puzzling increase in blood glucose at both 0.75 and 1.25 U/kg. Has anyone else had a problem with insulin efficacy of late? I can't believe all our mice are suddenly insulin resistant!

I am currently working towards developing a model for type 2 diabetes, using fructose and streptozotocin (STZ). Prior to injecting my C57BL/6 mice with STZ, we tested blood glucose with a tail-prick, using an AccuChek Instant glucometer. As per methods used in relevant literature, I fasted my mice for 4 hours prior to checking blood glucose (BG). The mean BG in my normal control, which did not receive fructose water was around 8 mmol/L, a BG akin to mild hyperglycaemia. Bear in mind, this group is not due for induction, and received a normal diet of commercially available mice pellets. For the second BG test, I fasted my animals for 6 hours, and got the same if not similar results.

Please assist.

My study requires the induction of STZ in 30 Sprague-Dawley rats (either sex) to develop type 2 diabetes. My protocol was:

1) Fast the rats overnight.

2) Prepared STZ solution in ice-cold citrate buffer (0.1 M, pH 4.5) and injected i.p. within 5 minutes of dissolving it. Dose used was 50 mg/kg.

3) Fed them with 10% fructose water for 24 hours post injection and gave food ad libitum.

4) Checked for blood glucose levels (BGL) 4 days after injection. Rats were fasted for 24 hours before checking the blood glucose levels.

When compared to the normal BGL, some of my rats had their BGLs around the normal fasting BGL range (4.0 - 6.5 mmol/L) while some were above 11 mmol/L.

Could there be any explanation to this? I had my supervisor's help to inject the rats as he has mastered the i.p injection technique and thus, error in the technique could be ruled out.

We measure the mouse blood glucose level by the normal human blood-biochemistry Roche mechanism. But we get the absolutely wrong value of the blood glucose level.

Normal 10 weeks C57BL/6 mouse (DO NOT have any treatment). Mean of the glucose level is around 40 mM. There must be something wrong.

World Health Organization — Diabetes, a chronic condition, is diagnosed and monitored with blood glucose testing. Learn more about the different types of diabetes on WHO's Official Website. Advice for the public.

My study focuses on developing a mouse model for the type II diabetes, narrowing in on a condition resembling chronic, late-stage diabetes. As such, blood glucose levels would need to be measured consistently over the course of the 16-week experimental period. I will be using C57BL/6 mice. With the welfare of the animals at the fore, I would like to know if it is safe to perform fasting blood glucose tests on a weekly basis. Will it overstress the animals, and perhaps serve as a confound the overall development of the condition?

Can one have a hyperglycemic coma? (I thought it was only for hypo)

I was back in the hospital after a syncopal episode again. Blood glucose 420-550

We are currently using Ketamine and Xylazine to anesthetize the mice for imaging purposes. However, upon injection, we see an increase in the blood glucose levels, which in turn, prevents us from inducing glucose stimulation at our desired time point. Is there any anesthetic material that doesn't interfere with pancreatic beta cells or elevate blood glucose levels?

Any insights will be very helpful.

Hi guys,

I'm working on a mouse model (using C57BL/6J) that needs high-fat diet (HFD). Recently I plan to perform ipGTT, just testing blood glucose. My colleague thought 1g of glucose/kg body mass was fine.

But some research suggested a higher amount of glucose for those mice fed with HFD.

One study recommended 1.5-2g/kg. (doi:10.2147/DMSO.S234665)

Another study suggested 2g/kg, but orally. (doi:10.1152/ajpendo.90617.2008) Can anyone explain the reason why we should choose a higher dose of glucose? Or maybe 1g/kg is also appropriate?

Many thanks. .

Hello.

We are currently observing the in vivo environment of mice at different glucose levels and

For this reason, we are currently measuring blood glucose levels in mice that have been administered anesthesia beforehand and treated with an open abdomen.

When we perform the laparotomy, we inject 30% glucose through a needle into the stomach of the mice.

However, the blood glucose level did not rise at all even after waiting 30 minutes after administration, and the mice died during the procedure after being warmed up to the 38°C range with a heater.

We are looking for a way to raise the blood glucose level in order to finally observe the glucose uptake in the pancreas, but do you have any good ideas on how to administer it?

Incidentally, we are currently considering direct glucose administration via tail vein, but no one has tried it and we are still in the consideration stage.

I will collect blood samples from streptozotocin induced diabetic model mice and I want to measure their blood glucose level from plasma. Which tube is best without any interference specially I will then use the GOD-POD enzyme spectrometric method to measure the blood glucose level.

I used 0.5 mg/kg glyburide in 1%DMSO for diabetic rats but it doesnt reduce blood glucose and i dont know why ?

rats was injected by 50mg/kg stz for diabetes induction

To calculate the sample size, I need to assume the smallest "r" with clinical relevance (error = 5% and power = 80%). My question is, which is that value? I searched for other papers to interpret correlation strength properly, but I had no results. Could you please suggest any?

I have collected data from 9 participants during the phases. The first phase the participants were asked to record their sleep throughout the night and fast from the evening before the lab. Blood glucose was then tested for 3 hours following a sugary drink. The second phase the participants were asked to restrict there sleep to 4-5 hours the night before and fast as well. The no participants would then have their blood glucose tested for 2 hours after consumption of sugary drink. The third phase the participants sleep was restricted and while in the lab they were asked to consume caffeinated coffee. Their blood Glucose was tested after the consumption of the sugary drink.

This study will therefore answer two important questions: (1) Does caffeinated coffee intake further impair blood sugar control after acute sleep restriction? And (2) Does caffeinated coffee supress appetite when consumed following sleep restriction? The participants were asked to fill out a 5 question Sprite questionnaire before each blood glucose test.

There are plethora of data on the concordance or dis-concordance between Fasting Blood Glucose and Hb1Ac in the clinical diagnosis of Type 2 Diabetes (T2D). Which of the two assay or test is the most specific and sensitive in the diagnosis of T2D

Does HOMA measure insulin sensitivity or resistance?

My SD rats have fasting blood sugar (16-25 mmol/l) after 6 weeks of  STZ-induced diabetes (60 mg/kg) . Can anyone give me the range for FBG in STZ-induced rats?

I am doing a machine learning project which determines the blood glucose level using laser light. I am training my data by actually measuring blood glucose levels using a glucose meter and taking the value of laser light passed through the fingertips, also taking into account the factors such as age and gender. So, during testing, my model will take 3 inputs, light intensity of laser light that has been passed through the finger, age, and gender, and will give the value of blood glucose level as output. Given that the input and output variables do not have a linear relationship, which machine learning algorithm will suit best for this problem.

I heard a lot of rumours that cinnamon helps a lot about reducing the level of sugar in the blood. Any confirmation about this idea?

Blood glucose testing is an integral test to measure glucose homeostasis in the body. It is commonly used both for screening and diagnosis of diabetes. In addition, serial blood glucose testing, also called "self monitoring of blood glucose (SMBG)" is a common tool performed by patients with diabetes for the assessment of their glycaemic status. However, blood glucose testing represents an unpleasant experience for many people. Can salivary glucose be used as an effective alternative to blood glucose testing for screening, diagnosis and follow-up of diabetes?

Hi all,

We are trying to establish a mouse model of diabetic nephropathy (DN) using the injection of 40 mg/kg of STZ in five consecutive days. Although the blood glucose levels were increased (300-600 mg/dL), they showed very low albuminuria and no sign of kidney damage in pathology passements after 3-5 months. We used two mouse strains, DBA2/j and C57 males, but we could not establish the model in none of the strains. Would you help us how to solve this problem?

Best Regards,

Maryam Abedi

We perform research into diabetes using insulin that may lead to impaired awareness of hypoglycaemia. We run a protocol to induce recurrent hypoglycaemia in rats using insulin over several days (currently to a max 13 concurrent days, or four weeks of three hypos per week). This involves taking blood samples for hormone analysis at least twice on a few hypo sessions (~200 µL is considered a good volume), and performing blood glucose tests up to three or four times per hypo (lance the tail to obtain blood droplet for blood glucose meter test).

Blood samples are taken through a scalpel cut near the tail tip.

Blood glucose is through the same wound, or using a lancet, also near the tail tip.

After a few days the tail becomes very sore and makes it very difficult to get samples, and the stress to the animal may confound the results.

Is anyone else doing rat work that requires this frequency and similar blood sampling?

Do you know an approach that is less impactful for the rat, staff and results?

Many thanks

Julian

In 2002, Dr. Kratz published a study of laboratory results from marathon runners with an average age of 49. Almost 60%or 22 out of 37 runners sampled from the Boston Marathon had a blood glucose level outside of the normal reference range (70-110 mg/dL) (Kratz)

A study from Austria of still older endurance athletes in their sixties found 47% were either prediabetic (22 out of 47) or diabetic (1 out of 47). In this small sample, these athletes had a higher proportion of hyperglycemia, compared to a heavier, age-matched group of controls, . (Haslacher)

From my review of the literature, this phenomenon may be due to increased hepatic release of glucose. Dr. Drouin found increased glucagon receptor sensitivity stemming from increased hepatic glucagon density in age-matched trained/untrained subjects.

I want to examine the relationship between the prevalence and risk factors of diabetics and socioeconomic factors. But doesn't able to locate Fasting Blood Glucose measurement in DHS dataset. How it is possible to measure?

it is under a module toxicology and biosensors

Hello everyone,

In order to study the effect of a certain drug on blood glucose, 10 patients were randomly selected and their blood glucose and insulin levels were measured for 5 days in a row. If you want to know the correlation between insulin level and blood glucose, how to analyze it?

Hi, as stated. Can you still used an expired Fluoride-Oxalate tube for blood glucose and lactate examination?

I notice that there might be a problem in getting blood sample because the tube's vacuum might not work as well. But would the result of blood glucose and lactate examination be reliable if the tube had been expired for around 1 month?

Insulin is the only known hormone that can directly promote anabolism and reduce blood glucose levels.

I am curious as to whether there exist other hormones with the same function as insulin? If so, how would you identify and examine the effectiveness of this hormone? Please list any detailed techniques and methods if possible.

Note: I would prefer not to use algorithms as this is only a small project. Please guide me through this, thank you.

I gave C57BL / 6 mice a solvent containing 17% DMSO by 0.1 mL i.p injection for 28 days. I saw that their weight and blood glucose levels decreased. Does anyone know the effect of DMSO on glucose metabolism?

With the increasing prevalence of diabetes world wide, and with the presence of high proportions of undiagnosed diabetes, do we need to perform annual fasting blood glucose testing as a screening step for undiagnosed diabetes.

Hello. I have a dataset containing three groups: low cholesterol, medium cholesterol and high cholesterol. Each group has about twenty participants. For each participant I have their mean nocturnal blood gluocse level for 4-6 different days. I would ideally like to compare mean nocturnal blood glucose levels between the three different cholesterol groups. I'm aware that unless I take a mean nocturnal blood glucose across the 4-6 days for each participant and then compare these values then using a one way ANOVA would violate the assumption of independant samples, but doing this would lose a lot of useful data. Is there a way that I can include all of the participant's individuals nights in a comparison between the cholesterol groups? Many thanks

At what minimum levels of rabbits and rats' blood glucose will lead to a coma? How much minimum glucose levels required for rabbits and rats alertness

I induced diabetes with STZ (40 mg/kg) after 4 weeks of high fat diet in Wistar rats. After 72 hours, i checked the FBG of all the rats and majority of the rats had FBG above 350 mg/dl. After a week i rechecked the FBG of the diabetes model group and i discovered that their blood glucose had dropped to normal, between 105-145, while the groups that are receiving treatment still showed higher FBG. Three days later i rechecked the non-fasting blood glucose of the diabetes model group and the least value was 480 mg/dl. Over the course of 3 weeks i discovered that their FBG is usually less than 150 mg/dl while their non fasting blood glucose is usually above 480 mg/dl. I have re-injected STZ but this phenomena is still the same. Does any one have any suggestion or experience about this situation

Adherence to frequent blood glucose testing by patients with diabetes via self monitoring of blood glucose assumed critical importance as it enables both patients and their physicians to have a view on blood glucose profile. This is of utmost importance in patients treated by insulin and/ or agents with hypoglycaemic potencies. However, many patients dislike frequent traumatic blood glucose testing and find it too uncomfortable and even painful. This is especially the case in children and adolescents. For this reason patients not infrequently deny testing which may adversely affect the management of diabetes. To overcome the problem of traumatic finger pricking to test blood glucose, several non-truamatic devices for self monitoring of blood glucose, totally avoid piercing the skin, have been discovered and marketed and found their way to practical use. Can painless methods for self monitoring of blood glucose increase the adherence of patients with diabetes to blood glucose testing?

Can you tell me if we use a human glucometer to measure blood glucose in rats, then what is the normal blood sugar level in rats?

I have only female mice and blood glucose do not exceed 170 mg/dl

while male showed results more than 250 mg/dl in previous experiment

If values of a specific feature for all samples in class A are statistically significant than values of the same feature of all samples in class B, would that give me any information or intuition regarding the outcome of classification? For example if you measure the blood glucose of 100 obese mice and in 100 normal mice and do ANOVA and find that the glucose level of obese mice is statistically significant than normal mice, would that give you any info about the output of classification?

The dose I use - 65mg/kg i.p.:1.The rats were approximately 240 mg

2.they were on fasting 12-16 hrs before injection

3.After induction of diabetes they were given sucrose(15g/L) solution for 48 hrs.

3. I didnt use slow acting human insulin, while i need untreated rats for control group.In 2 days 25% of rats were dead, 75% have got blood glucose 31-33 mM/L . After 4 days of induction the most were dead.

If blood samples are to be stored frozen for some time till the analysis of blood glucose levels .. is it accurate to use frozen serum or it must be stored in grey tubes ? For how long ? And does it need any processing in the grey vacutainers before storage?

Dear researchers/professors,

For my master's thesis I prepared insulin loaded nanoparticles (100 IU/kg) and gave them to rats via oral route. To compare my formulation I also gave insulin sc (2 IU/kg) to rats and collected their blood. When I measure the blood glucose level I saw the change and I put the blood in eppendorf tubes that has heparin inside. After centrifugation I collected plasma and used human insulin ELISA kit. The calibration solution created the color perfectly but my samples gave no color or absorbance so I wanted to get your opinion about what might cause this failure?

Thank you.

This is about a different approach to fighting cancer basically cancer cells must have glucose to survive. Following a ketogenic diet, the glucose drops and then using the administration of diabetic drugs to drop and keep blood glucose levels below 60 mg the healing process should start.

I would like to ask if you know people who tried this diet approach as detailed as possible & in the first place can somehow affect the patient they are any circumstances for that?

Can be this a way to heel cancer ? Are there researches that confirm this process or is the book written without evidence and is everything in theory?

Regards,

What is the injectable range of insulin for daily administration to normalize high sugar level in Albino rats?

What is the Blood glucose range for rats?

We're presently working on the effects of monosodium glutamate on body weight & blood glucose levels; the experimental animals are wistar rats.

We administered it in solution during the am hours but they didn't seem interested in drinking the MSG-laced water until during the pm hours.

How best do we feed them MSG daily and at what time of the day?

I have a list of blood glucose levels before and after a missed dose. These are compared the optimal range. I would like to obtain a p value in order to see whether the missed dose had a significant impact on the blood glucose levels.

Estimation of HbA1c based on the blood glucose values.

I am planning to find the integration of the blood glucose readings over time and use the result to find the HbA1c value.

I am sure TIND is real, but I am wondering why the German guidelines of diabetes treatment seem to ignore the facts and allow freshly diagnosed patients with high Hb1Ac to reduce their blood glucose fast and risk pain, suffering and, possibly, induce severe complications of too fast reductions of blood glucose. How is it in other countries, are there more modern recommendations?

short report: http://www.neurologytimes.com/diabetes/treatment-induced-neuropathy-diabetes-underrecognized-rise/page/0/1

So, we are trying to induce T1DM, and need to measure the blood glucose of the pregnant female mice. Rather than cutting the tail (we were guessing this may interrupt their pregnancy), can we measure blood glucose level in amniotic fluid during the harvest if embryo instead?

We want to measure the blood glucose in amniotic fluid at E14.5.

And I'm wondering, at this stage, whether the blood glucose is more closer to the maternal BG, or the embryonic BG?

Or in other words, at what stage, the BG of amniotic fluid is still similar to mother's BG?

Actually I had fed HFD to C57BL/6J with or without a drug. The result of that experiment I got that the drug suppresed HFD-induced metabolic syndrome according to body weight, fasting blood glucose, GTT, ITT, energy expenditure and so on. But one thing is not matched with all of other phenotypes. I fed HFD for more than 22 weeks and epididymal fat tissue mass was smaller than drug treated mice. Also HFD only fed (non drug treated) mice's eWAT show that some part of tissue looks like yellow and hard and severe inflammation. I think it is come from long-term HFD-induced fibrosis of fat tissue. Is that right?

If someone knows about it please let me know. Even already reported reference paper is also good for me.

Thank you.

I am 33 weeks pregnant and a type 1 diabetic. I have been told by doctor's that I am already starting to have contractions but no dilation or indications of delivering soon. Since the contractions could have started my blood glucose levels have been impossible to bring down to an acceptable level or difficult to increase to an appropriate level. Any advice on how to better control the changes in my blood glucose level, as they change at random times during the day?

Dear all, I am interested in establishing model for gestational diabetes. I used alloxan (i.p. 120mg/kg) to induce diabetes in female rats. Does anyone has experience about the reversibility during pregnancy? How to maintained hyperglycemia if there is restoring of blood glucose level? Another alloxan injection?

Hypoglycemia is a condition with the blood glucose level below the threshold of 70 mg/dL [1] and is considered serious adverse effect of insulin based treatment for type1 diabetes (T1D) patients. One of the earliest manifestations of hypoglycemia is the involuntary shaking of the human body such as the fingertips.

Paper:

R. McCrimmon, “Glucose Sensing During Hypoglycemia: Lessons From the Lab,” Diabetes Care, vol. 32, no. 8, pp. 1357–1363, Aug. 2009.

American Diabetes Association Workgroup on Hypoglycemia, “Defining and Reporting Hypoglycemia in Diabetes: A report from the American Diabetes Association Workgroup on Hypoglycemia,” Diabetes Care, vol. 28, no. 5, pp. 1245–1249, May 2005.

I was looking into different literature and I want to know if there is a guideline for fasting glucose level for OGTT glucose level. It seems like mice with >8mM (fasting) and >7.8mM (OGTT) are considered diabetic 

What are the best statistical tools to analyse the reliability and validity of a diagnostic instrument?. Which gives the data in the ratio scale and the instrument is used for measuring the as body weight, blood pressure, blood glucose etc.

My research domain is "Blood glucose monitoring and insulin suggestion for diabetic patient". Is there any suitable data set OR i can generate my own by using any blood glucose model ?

I'm starting a research on mice to measure HbA1c levels and would want to get results in a few weeks (6-10weeks).

Your OGTT curves suggest that 4-h blood glucose concentrations dropped below fasting even in controls? That does not look real does it?

Hi

I'm running a small study to compare between the readings of different finger-pricking devices that measure blood glucose levels. Each study participant takes two measurments before and after their breakfast (two blood glucose readings, one from each device). So that's 4 readings per day for a total of 12 days = 48 data points for each participant.

What kinds of graphs do you suggest I plot? And what statistical analysis is applicable, aside from Bland-Altman Analysis?

Hi everyone,

I would like to ask, is it possible if resistant maltodextrin or other resistant starch increases postprandial blood glucose? Is there any synergestic interaction between starch and fiber?

Whats the reason behind the diabetes incidence (blood glucose level less than 250 g% in db/db mice (of less than 50% of the animals ) in a db /db mice on B6 background even after 8 weeks of either sex but having obese phenotype ?

Does circulating blood glucose concentration limit rates of aerobic ATP production. i.e. can supplementation of glucose result in an increased ATP availability during recovery from aerobic exercise?

why extract of okra lowers blood glucose and serum lipids?

We are running hyperinsulinemic euglycemic clamp in mice. But I have always been thinking about what does euglycemia means. Should the fasting glucose value of each individual mouse be the guide for the blood glucose you want to achieve at steady state or should you decide beforehand that every mouse should achieve the same blood glucose values at steady state of the clamp. If a group of mice have lower fasting glucose value than another group, then this first group might need lower GIR to maintain their “own” euglycemic value, but if you would have clamped all mice at the same blood glucose values the group with lower fasting glucose values will probably have a higher GIR. How do you usually do in mouse experiments using the glucose clamp technique?

there are several methods to making animal model of diabetes, but if we want to make a model of diabetes with desirable blood glucose, how it is possible?

rat blood glucose level

I need data contains blood glucose , blood pressure and location of patients during a day.

I'll be so thankful to anyone can help me,,

here is my e-mail address:

To my knowledge we use Clarke and Parke error grid analysis to see analytical accuracy or clinical accuracy of the performance of blood glucose (BG) meter with different borders of the zones. My question is, can we use Clarke and Parke grid analysis for non-invasive glucose solution prediction? I am looking forward. Many thanks

I have given a bolus (drink containing sugar) and measured HOMA and QUICKI indexes post-bolus for up to 5hrs every hour.

I know both HOMA and QUICKI work on fasting insulin and glucose values, does this mean my data is invalid as I have given my participants a drink to consume?

I am estimating power for an experiment using AUC of blood glucose response over 120 minutes as the primary outcome. I'm struggling to find good quality reliability data for this, in particular I need an estimate of likely within-person variation. There's lots on GI but that isn't directly helpful.

In my laboratory we only have the conditions to use small rodents, rats or mice (or cell lines).

In your opinion, what is the best model for studying the long-term effects of gestational diabetes on the offspring? There is so much diversity of protocols and diets with such different compositions ...

is there any cut off value of blood glucose to say that a rodent is diabetic?

Thank you in advance for sharing your knowledge

I have read several articles that aloe vera has antioxidant properties that has the potential to lower blood glucose levels. I was wondering if there are other plants that could have the same properties to serve as an alternative.

In a clinical setting to measure fasting blood glucose, is it better to use serum or capillary blood?

I have 2 rat experimental groups. Their weights were taken before and after 17-18h starvation. 

Group1 showed less body weight loss and a tendency of increasing of blood glucose level compared to the Group2.

Can anyone help me to understand what it means, please?

Thank you so much.

Best Regards,

Mario

I need some sample papers

animal is induced using stz (40 mg/kg) and nad(110mg/kg). when we checked the fasiting blood glucose one day it has decreased week later it has increased. pls clarify.

3 days after the induction of the dibetes using nicotinamide (110mg/kg) and streptozotocin(40mg/kg) the animal blood glucose was checked and it was treated with metformin(300 mg/kg). Glucose check was done for every 7 days with animal being fasted for 6 hours. first week FBG was found to be 168, 100 and 274. Metformin treatment was continued.   after one week when animal checked with fasting blood glucose again it showed 600+.

I got a few blood glucose values below 50 mg/dl after a 16h fasting and wonder if anyone else got similar results. 

From RBC we can calculate average levels of blood glucose levels , over three months, using the hemoglobin A1c test and we can measure somatic levels of glutathione. Several formulas exist to determine levels of ionized calcium using serum Calcium, Phosphorus, and Albumin. Using erythrocytes, I posit, would provide an average of ionized calcium levels - instrumental in managing arthritis, cardiovascular pathology, and, finally, cancer 

I want to do an intervention. Info about web-linked test kits would be valuable.

HbA1c or glucose.