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Biotechnology
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I am a student of biotechnology and an independent SARS CoV-2 researcher from India. For finding the academia research status and analysis of the integration of innovation with research, I have created a set of Multiple choice type questions about your experience as a researcher. The google form requires nothing but your honesty and openness for research. Feel free to ask questions and DM. The questions will assist in gauging the level of innovation and writing in academia.
If possible, please do forward this little form to your fellow researchers and other amazing scientists. I would be highly grateful.
Thank you
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Hello sir, Asif Bilal
Just saw your response. It means a lot to me. Thank you so much for your time.
If possible, could you please forward the survey to other amazing scientists, It would help me a lot.
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Hi,
I wonder if anyone knows how to use sample release reagent from Sansure Biotech ?
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Hi,
The supplier claims that their "sample release reagent" (nucleic acid release technology), can lyse pathogens at room temperature very fast, with no need to heating, centrifuging or replacing tubes, the sample DNA/RNA can be extracted quickly through simple operations. The reagent is applied for the pretreatment of nucleic acid molecules, to release them from specimens, then the released nucleic acids can be used for clinical in vitro diagnosis or for the detection through appropriate apparatus.
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Long story short, I need to degrade 30ug of RNA and i need to do it at 4C. I want to use only as much RNAse A as necessary.
So if performing the reaction at 4C, how long of incubation time and how much RNAse A would i need to degrade 30ug of RNA?
For example, would 1ug of RNAse A(20ug/ml conc.) for 15min at 4C be enough?
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I agree with Kyle Skalenko that experimentation is needed but first contact the company to sort out whether they work in enzyme units or Kunitz units to define the activity of your rnaseA, Then assume that the activity drops by a factor of 2 for every 10c drop in temperature to get an approximation of how much enzyme or increased time will be needed
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Hi!
Dear colleagues and respected seniors, I am searching for a biochemical reaction to identify the km and vmax values for the said enzyme, which converts alpha carotene into Lutein, but still I am not able to identify, so if anyone could help to find it, give me a reference article/ paper please, I will be very thankful to al of you.
Thanks and best regards
Mr. Rafi Ullah Khan
PhD candidate (Biotechnology)
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Try looking through the references here:
Unfortunately, there are no kinetic data listed, suggesting that none of the references will contain a suitable assay.
If hydroxylation results in a measurable change in the absorbance or fluorescence spectra of the carotenoid, you may be able to follow the reaction by that means.
You may have to use a chromatographic separation to measure product concentrations, possibly combined with mass spectrometry if you can't fully separate the substrate and product chromatographically. The insolubility of the substrate may also be a problem, though, since detergents needed to keep the substrate in solution will could be incompatible with the analysis of the reaction by HPLC or LC-MS.
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Hello...
Two months ago, I bought pyrocystis fusiformis culture for my research. Unfortunately, no bioluminescence was observed, and the culture seemed to be deficient from the start.
Now I'm trying to buy a Pyrocystis fusiformis culture again and found a lab called PyroFarms, but before I order, I wanted to check if anyone had any past experience with them. or alternatively, if you could name any other trustworthy sources where I could buy a P. fusiformis culture, I would be grateful.
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Pyro Farms, highly recommended
Joel
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As all the world is busy in discovering cure for COVID-19, so why not radiation? COVID-19 has been reported to effect the human's lungs and liver (specific tissues), So, what if those parts are exposed to radiation for the rapid recovery of the patients? Is it possible? Or, is there any possibility for radiotherapy of COVID-19?
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I couldn't see much paper where plant breeders use biochemical such as proline content Malondialdehyde (MDA) and dyes such as NBT or DAB( for ROS detection) for screening stress-tolerant accession on a large scale (100-200 which I suppose is possible to do). Are not these methods better than phenotyping grain yield, biomass, plant height, NDVI, LAI, etc?
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For the genome selection and have a good final product you may arrange a true and real programme of plant breeding.
I recommend to go to CYMMIT and ask for one of the excellent programmes that they have in their research center.
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I am Janu Newar, Ph.D. Scholar from School of Biotechnology, KIIT University, Bhubaneswar, Odisha. Our lab is basically working on proteins from biological sources. This work involves the identification of some proteins. I would like to request some help with the Agilent 6530 Q-TOF instrument.
For identification of the protein, we are using a glass insert of 0.2ml volume (Part no: 5188-5390). My query is can we use 0.025ml of the sample volume in this insert and can inject 0.02ml of sample in the column. If not, then how much lower volume can we use in this 0.2ml insert?
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You may need to play around with the needle depth setting in the method, so the needle would go fairly close to the bottom without actually touching the bottom. Also reduce the draw rate so the liquid on the side wall has the opportunity to coalesce.
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I have 100 uM concentration of ssDNA-FQ with molecular weight ~ 2400, and100 uM of 6-FAM with a molecular weight ~376. While performing a fluorescence assay, I get higher fluorescence intensity with cleaved ssDNA-FQ than 6-FAM. I am not sure that with the same concentration of ssDNA-FQ and 6-FAM whether I have the same number of molecules or not. Has anybody experienced anything like this before? Please let me know. Thanks in advance.
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Srisruthi
The extiction coefficients (Abs) can be different when Fam attaching to different molecules. The concentration of ssDNA-FQ (Fam lablled ssDNA, right?) can be determined by abs@260(DNA) or by abs@498nm(Fam), this may result difference in concentrtion.
The Fam you used may be only 80-90% pure, with some no fluorescent impurity in.
For concentration of dye, extiction coefficient is used to dertimined the concentration.
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I want to do dialysis purification for the cyclic peptide 2K after its modification using membrane dialysis MWCO 1K but I am worry that the cyclic structure has a smaller size than its linear one and ca pass through the membrane.Is the cyclic structure has bigger or smaller size compared with the linear structure?
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Cyclization significantly reduces Stokes radius. Adam B Shapiro is right. I sometimes include 1% HOAC for the desalting column, as it decreases non-specific binding.
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Direct experiences with Biotechnological and Biomedical Approach? Therapies, long-term implications?
I'm not looking for definitions but protocols and methods that you personally have made and put into practice. Any information you have is welcome.
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masala nimada
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By using this cytokine kit I am getting high absorbance value in my positive controls. Recommended values for positive controls are 1.5-2.5, but I am getting values around 3.0-3.5.
In some of my negative control I have absorbance more than the experimental sample. Eg. negative control value 0.04 and sample value 0.01.
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hello everyone,
how could I calculate the results of my patients using Qiagen Multi-Analyte Elisarray Cytokine kit?
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I will start an SSF for fish and I want to know what is the best inoculum size to start with ? Is that different from one bacteria strain to another?
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There is what we call optimization, especially with response surface methodology (RSM) or artificial neural network (ANN). With this method, I determine how much of every factor is required to significantly contribute to the biosynthesis of a microbial metabolite. I could give you a list of my materials. Whether you use Bacillus or not, a lot depends on the phase of production; whether growth-associated or non-growth-associated.
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I am planning a fluid dynamic experiment and wonder what tubing material is biocompatible for cell culturing experiment. Also wonder if there is a tubing material that can be sterilize.
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Len Leonid Mizrah Thank you for the details that you provided, I will check it.
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What are the limitations and disadvantages of Real-Time PCR (RT-PCR)?
What is a more specific and sensitive technique that can be used in the laboratory instead, particularly in cancer diagnosis?
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Limitations of End-Point PCR
Agarose gel results are obtained from the end point of the reaction. End-
point detection is very time consuming. Results may not be obtained for
days. Results are based on size discrimination, which may not be very
precise. As seen later in the section, the end point is variable from
sample to sample. While gels may not be able to resolve these
variabilities in yield, real-time PCR is sensitive enough to detect these
changes. Agarose Gel resolution is very poor, about 10 fold. Real-Time
PCR can detect as little as a two-fold change!
Some of the problems with End-Point Detection:
 Poor Precision
 Low sensitivity
 Short dynamic range < 2 logs
 Low resolution
 Non - Automated
 Size-based discrimination only
 Results are not expressed as numbers
 Ethidium bromide for staining is not very quantitative
 Post PCR processing
As like the conventional PCR, there are three main steps in real-time PCR;
Denaturation
Annealing
Extension
Denaturation occurs at 94°C where the double-stranded DNA is denatured and two single-stranded DNA is generated. The DNA is melted.
This single-stranded DNA is the sight of the annealing for the primers in the later step of the amplification.
Annealing occurs at 55°C to 66°C in which the sequence-specific primer bind to the single-stranded DNA. Along with it, the fluorescent dye or the probe bind to the DNA sequence too.
Extension occurs at 72°C at which the Taq DNA polymerase activated highest. In this step, the Taq adds dNTPs to the growing DNA strand.
Note: if the amplicons are less, combine the extension step with the annealing step (for real-time PCR only).
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Novozyme 234 is the best enzyme for the isolation of protoplast but in recent days the Novozyme 234 are not commercially available. So is there any other method (enzymatic) for this isolation?
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You can use chitinase and cellulase CP.
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Dear scientists and researchers. I ask those who have knowledge of the names of Free of charge, indexed scientific journals in the field of biotechnology; gives us links to those Journals.
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There are good free Biotechnology Journals. I suggest you access the following pages below. They are specific tools of the best publishers, which enable authors to find appropriate Journals for their submission. It's easy to use, briefly, you add the title and abstract of the paper and some Journals that publish works within the scope of your work will be suggested.
I hope I helped you.
Journals Elsevier Finder
Wiley Online
Taylor & Francis
Springer
Yours sincerely,
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The bio-technologies today are becoming important part of the industry and science research development and innovation. In case of brain tumor or injuries, apart from surgical intervention, the data may also be collected via noninvasive bio-signals such as EEG, transmitted, stored and analysed in e-Health Electronic Health Record. What are future research directions in bio-technology driven bio computing?
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This question is very wide, hence, in the following, it gets narrowed into two specific areas: biosignal prediction of ECG recordings, and the theory of robust massive parallel computations.
We arrived at the point when complex systems measure enables us to distill a very rich set of information from biosignals. As an example, in some cases the information provided by ECG recordings is so rich that we can predict arrhythmia up to one hour before their actual onset in a rabbit model, see the paper of Kroc & Bobir.
The second very deep area of my research deals with developing a better understanding of robust emergent computations that occur in massively parallel computational environments, including biology. In plain words, the main problem in all biology describing mathematical models is their lack of resilience to the faults occurring within the underlying, unreliable computing medium.
This topic get studied in the paper dealing with a cellular automaton called the 'Game of Life' and its robust generalization called r-GoL, see the relevant paper when interested and Python code available on the RG profile. Surprisingly, a cellular automaton that is highly resilient to the faults of underlying computing massively parallel information processing medium was found.
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Couldn't find any fresh publications explaining this. Also nobody usually says what strand encodes all ORFs of HBV.
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HBV genome (DNA) is organized in a highly condensed manner and all the genes are encoded within four partially overlapping open reading frames (ORFs), namely as; S (surface), C (core), P (polymerase) and X (X protein) and include 7 proteins: HBcAg, HBeAg, HBx, polymerase (pol) and the three different sizes HBsAg- the small (S), medium (M) and large (L) proteins.
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Probiotics can be used as dietary supplements or drugs to treat diseases. The genetically modified probiotics will offer much more beneficial features than the wild type probiotics. Does FDA approve genetically modified probiotics? When do you think they will approve? What scientific questions do we need to resolve before FDA can approve? Can we use genetically modified probiotics as dietary supplements without FDA approval?
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The GM probiotics, like other GMO, have both their pros and cons depending on the method (s) used to make them.
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Is it possible to use Artificial Intelligence (AI) in Biological and Medical Sciences to search databases for potential candidate drugs/genes to solve global problems without first performing animal studies?
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We hypothesize that future generations of Artificial Intelligence (AI) technologies specifically adapted for biological sciences will help enable the reintegration of biology.
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Can anyone suggest a method to quantify proteins in melanin containing samples?
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Please refer to the research article given below.
Highlight:
Using several assay methods, synthetic eumelanin prepared by autooxidation of L-beta-(3,4-dihydroxyphenyl) alanine and a natural melanin isolated from dog hair melanosomes were tested in model experiments to assess their possible interference in protein determination. The degree of interference was assessed by comparing the data obtained with the melanin samples with those derived from measurements of bovine serum albumin. In the common biuret and Lowry methods melanin interferes by falsely increasing the values obtained; the addition of Folin reagent only after melanin removal, as suggested by Doezema, decreased but did not eliminate melanin interference. Methods working at acid pH such as those according to Salo and Honkavaara with Ponceau S or Sedmak and Grossberg or Spector using Coomassie blue G-250 proved much better.
Although melanins adsorbed a small amount of dye from the reaction systems in these procedures, their sensitivity to proteins makes the melanin interference negligible. Such procedures can therefore be recommended for protein determination in the presence of melanin.
References:
Sedmak and Grossberg.
Spector
Best.
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Dear all,
I am re-writing now my previous question.
Are you aware if there is any protease with very low efficiency to cleavage IgG ?
Are you aware of any publication?
Regards,
Daniel
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Papain or other specific protease-functionalized gold nanoparticles may be useful.
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Our quantum dot(g-C3N4/CQDs) was prepared from melamine and then exposed to phenylbronic acid(PBA) for the next steps of the experiment - whose fluorescent properties were quenched (Figure 1) but after 5 days and quantum dot solution dialysis with PBA (1000D) A little of its fluorescent property back, what is your analysis?
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Is there a scientific journal concerned with biology, microbiology, biotechnologies or medicinal plants, issued monthly or every two months, and is ifree of charge?
Is there an accredited scientific journal concerned with biology, microbiology, biotechnologies or medicinal plants, issued monthly or every two months, and is it free of charge?
With thanks
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Journal concerned with biology, microbiology, biotechnologies
1. International Journal of Microbiology and Biotechnology
2.Journal of Microbiology, Biotechnology and Food Sciences
3.Applied Microbiology and Biotechnology
4.World Journal of Microbiology and Biotechnology - Springer
5.Journal of Applied Biology and Biotechnology
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I am working in molecular diversity and want to make a dendogram, what is the best software easily available for this?
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PAST - a free software for scientific data analysis is easily available and a very neat software...
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I am trying to get CRISPR/Cas9 working in my lab. I have a strain of Aspergillus that expresses the Cas9, and I need a way of introducing the gRNA.
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George Matthews thank you very much!
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When we are focusing on our interest in the field is obvious to affect the GPA but is said that the GPA is prime for the selection characteristics for foreign study does it really matters or it is a myth?
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I agree Pallavi manna
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I dissolved indomethacin (Santa Cruz Biotech - SC-200503) in DMSO and prepared stock of 25 mg/mL
In my study, I inject 10 mg/kg i.p., injection volume 0,5 mL and I work with 250-300 g rats.
When I diluated the stock solution with saline (%0,9 NaCl), the diluated solution has been crystallized.
How can I avoid that?
Open for any suggestions.
My kind regards.
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What makes biotech stocks spike? will it be a good case, if you are trying to create a big company and sell its shares on stocks markets1?
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Hi,
I want to remove thiol molecules on a gold substrate. After searching, I found couple of method described below;
1. NH4OH–H2O2–H2O solution aim is to initiate the oxidation of the thiol compound
2. Thermal desorption (above 200 ◦C)
3. Ultraviolet light and ozone (O3)
Do you have any experience about these methods. If your answer is yes, can you share your experience with us? Thank you for sharing and helping,
Best Regards,
Osman
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I would recommend going for ethanol washing followed by oxygen plasma cleaning under a vacuum.
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I read several papers where researchers use several strains of the same species for analysis of recombination or selection pressure however there are few papers in which researchers use the multiple species belonging to one 'Genus', not the multiple strains that belong to one 'Species'. What difference is expected in the result and how it can be interpreted.
I want to know how my pathogenic organism 'Xyz abcde' is evolved (recombination and selection pressure). 5 different strains of 'Xyz abcde' is sequenced while 9 organisms in Genus 'Xyz' is sequenced.
Which dataset I should use to proceed and why? How it will make difference in the analysis?
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The answer is found in this study:
J Virol. 2006 Nov; 80(22): 11124–11140.
Published online 2006 Sep 6. doi: 10.1128/JVI.01076-06
PMCID: PMC1642140
PMID: 16956935
Recombination and Selection in the Evolution of Picornaviruses and Other Mammalian Positive-Stranded RNA Viruses
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Genomic and biotechnological studies, as mentioned, using RNA polymerase and RNA are preferred over DNA studies (using DNA and DNA polymerase II) because the RNA polymerase does not need a primer(?) Would a primerless DNA polymerase II not need an an RNA primer.(?)
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oh, there is one. or better said, there are two. the first one was posted by Liang Jiang earlier. the next one is here
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crRNA is responsible for recognizing and binding the sequences next to protospacer-adjacent motif (PAM), NGG, on the target DNA, whereas tracrRNA is essential to maintain cas9 nuclease activity. and most of the miRNA does not contain the PAM sequence (5’-NGG-3) 4. so how can you target them by using CRISPR Cas system?
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thank you dear Dr Sun Xin for your suggestion
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Metabolic rewiring and epigenetic remodeling, which are closely linked and reciprocally regulate each other, are among the well-known cancer hallmarks. Studies have reported use of Onco-metabolites to metabolically reprogram the epigenetic of cancer. I was wondering what might be major limitations of such techniques?
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Hello. This topic is not exactly my field, so I cannot give you a satisfactory answer. I will be happy to follow all the news and discussions in this field.
Regards, Zlata Felc.
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in cancer therapy, sometimes you have to target multi-targets to understand the molecular pathway of the specific protein. CHyMErA can target multi targets but it is not safe and has a high risk of extra mutation!
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thank you dear Dr Murali Mohana Rao Singuru
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I am working on a Plastic Bioremediation Project and need to pre-treat the plastic waste samples before further work can be done.
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Biswajit Debnath Thank you so much! The chapter has everything I need.
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These are the structures from plant powders when observed under binocular microscope. Can anyone help me identify these
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Just try to add more data and also a scale bar. That will help.
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Disclaimer: I am not a microbiologist by profession or training and this is a curious/knowledge question
I have read some old articles that suggest the inoculum concentration to be 10^5 according to EUCAST.
I have also read some review articles suggesting 10^8 (0.5McFarland standard)- citing CLSI.
I am not able to download CLSI guidelines, can anyone help me get a copy to read about broth dilution and initial cell density actually prescribed?
Please let me know if these standards have changed? What cell concentration do researchers commonly use to read MIC (in North America or elsewhere)
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Hi there everyone,
I'd like to take your opinion from your experience and observation. I'm currently looking to advance my career. I work as a research assistant/technologist in Molecular Medicine and have an MSc in Biotechnology and BSc in Pharmaceutical Sciences.
What options are there (apart from PhD), to develop my career, and not always be an RA?
Thank you :)
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You can become an application specialist. If you have a sound knowledge of your subject, you can attend courses on biological techniques and instrumentation, and develop your skills further in latest instrument handling and techniques. Attend workshops and conferences organized by companies. Techniques in molecular biology (genomics) such as NGS or in cytogenetics are very much popular in academics and industry. Meanwhile, you can do an MBA course in your free time so that you can be well placed in your career. Not necessary that you should do a PhD. However, obtaining a PhD degree is always at an advantage as it will help you climb the ladder of success much faster.
All the best.
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I have a task which I need to recover rhamnolipid by using solvent extraction. I have gone through some papers but some of them using different solvent for rhamnolipid recovery, which are 1) methanol: chloroform: acetone (1:1:1 v/v) and 2) methanol: chloroform (2:1 v/v). Do they affect the rhamnolipid yield? Which solvent should I use?
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Transfections using chemical transfection reagents rely on electrostatic interactions to bind with nucleic acids and to target cell membranes. what is the best and efficient transfection reagent?
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For example, DNA has functional groups such as methyl groups and nanoparticles can interact with them and alter them. I was wondering if we have any technique by which we can quantify them or maybe do a qualitative analysis. Any leads would be great, Thank :)
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I have to measure the concentration of a specific protein inside the blood. And Unfortunately, our lab does not have enough facilities to perform western blotting, so can we use ELISA instead?
What is the best way or techniques to have accurate results????
Can ELISA be used instead of Western-Blotting to measure protein concentration?
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Chromatographic methods can also answer. MS or UV detection after appropriate seperation may also handle. Both methods are able to serve quantitative results after convenient sample preparation and protein isolation (generaly immunoaffinity based techniques at complex matrix such as blood)...There are hybride methods which combine ELISA preparation and MS detection (IA-LCMS). The easiest and most reliable way of doing chromatographic analysis of targeted protein is the quantitative proteomic approach if your facility has triple quad MS system. In this case you may pick a couple of signature peptides for your target after HRMS peptide profiling or by employing in silico digest to create theoritical peptides. Using Skyline software you can create MS method acqusition and it is ready to perform quantitative proteomic analysis for you protein which can be mentioned as highly specific...
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Would proteomics studies matter in drought tolerance screening for legume crop accessions?
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Hallo,
Proteomics research related to the effects of water deficits on plants might be productive, but only if done using the strictest methods to control and measure the water status of plants. May I suggest reading:
Genetic engineering to improve plant performance under drought: physiological evaluation of achievements, limitations, and possibilities
David W. Lawlor
Journal of Experimental Botany, Volume 64, Issue 1, January 2013, Pages 83–108, https://doi.org/10.1093/jxb/ers326
Which explains the problem and a way of dealing with it.
Regards
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Good research is based on good relationship between the mentor or supervisor and the scholar. What are the qualities a supervisor or mentor must have to have a healthy and friendly environment in the laboratory?
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Please have look on our(Eminent Biosciences (EMBS)) collaborations.. and let me know if interested to associate with us
Our recent publications In collaborations with industries and academia in India and world wide.
Our Lab EMBS's Publication In collaboration with Universidad Tecnológica Metropolitana, Santiago, Chile. Publication Link: https://pubmed.ncbi.nlm.nih.gov/33397265/
Our Lab EMBS's Publication In collaboration with Moscow State University , Russia. Publication Link: https://pubmed.ncbi.nlm.nih.gov/32967475/
Our Lab EMBS's Publication In collaboration with Icahn Institute of Genomics and Multiscale Biology,, Mount Sinai Health System, Manhattan, NY, USA. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/29199918
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Our Lab EMBS's Publication In collaboration with Bharathiyar University, Coimbatore-641046, Tamilnadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27919211
Our Lab EMBS's Publication In collaboration with LPU University, Phagwara, Punjab, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/31030499
Our Lab EMBS's Publication In collaboration with Department of Bioinformatics, Kerala University, Kerala. Publication Link: http://www.eurekaselect.com/135585
Our Lab EMBS's Publication In collaboration with Gandhi Medical College and Osmania Medical College, Hyderabad 500 038, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27450915
Our Lab EMBS's Publication In collaboration with National College (Affiliated to Bharathidasan University), Tiruchirapalli, 620 001 Tamil Nadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27266485
Our Lab EMBS's Publication In collaboration with University of Calicut - 673635, Kerala, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/23030611
Our Lab EMBS's Publication In collaboration with NIPER, Hyderabad, India. ) Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/29053759
Our Lab EMBS's Publication In collaboration with King George's Medical University, (Erstwhile C.S.M. Medical University), Lucknow-226 003, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/25579575
Our Lab EMBS's Publication In collaboration with School of Chemical & Biotechnology, SASTRA University, Thanjavur, India Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/25579569
Our Lab EMBS's Publication In collaboration with Safi center for scientific research, Malappuram, Kerala, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30237672
Our Lab EMBS's Publication In collaboration with Dept of Genetics, Osmania University, Hyderabad Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/25248957
Our Lab EMBS's Publication In collaboration with Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Hyderabad Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/26229292
Sincerely,
Dr. Anuraj Nayarisseri
Principal Scientist & Director,
Eminent Biosciences.
Mob :+91 97522 95342
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So my last year project is Drug Efflux Pumps and Persistence in Methicillin Resistant Staphylococcus aureus and we gonna focus on persister cells to study the path way of antimicrobial resistance...my question is how can i link bioinformatics and some coding to this project without requiring wgs cause it's not an option inside our lab !I need a small yet beneficial technique/ tools in small scale that i can learn and implement by my self .PS I love programming in general but im still new to bioinformatics so i need help to link my passion for coding and my field "biotechnology"
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Please have look on our(Eminent Biosciences (EMBS)) collaborations.. and let me know if interested to associate with us
Our recent publications In collaborations with industries and academia in India and world wide.
Our Lab EMBS's Publication In collaboration with Universidad Tecnológica Metropolitana, Santiago, Chile. Publication Link: https://pubmed.ncbi.nlm.nih.gov/33397265/
Our Lab EMBS's Publication In collaboration with Moscow State University , Russia. Publication Link: https://pubmed.ncbi.nlm.nih.gov/32967475/
Our Lab EMBS's Publication In collaboration with Icahn Institute of Genomics and Multiscale Biology,, Mount Sinai Health System, Manhattan, NY, USA. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/29199918
Our Lab EMBS's Publication In collaboration with University of Missouri, St. Louis, MO, USA. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30457050
Our Lab EMBS's Publication In collaboration with Virginia Commonwealth University, Richmond, Virginia, USA. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27852211
Our Lab EMBS's Publication In collaboration with ICMR- NIN(National Institute of Nutrition), Hyderabad Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/23030611
Our Lab EMBS's Publication In collaboration with University of Minnesota Duluth, Duluth MN 55811 USA. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27852211
Our Lab EMBS's Publication In collaboration with University of Yaounde I, PO Box 812, Yaoundé, Cameroon. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30950335
Our Lab EMBS's Publication In collaboration with Federal University of Paraíba, João Pessoa, PB, Brazil. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30693065
Our Lab EMBS's Publication In collaboration with collaboration with University of Yaoundé I, Yaoundé, Cameroon. Publication Link: https://pubmed.ncbi.nlm.nih.gov/31210847/
Our Lab EMBS's Publication In collaboration with University of the Basque Country UPV/EHU, 48080, Leioa, Spain. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27852204
Our Lab EMBS's Publication In collaboration with King Saud University, Riyadh, Saudi Arabia. Publication Link: http://www.eurekaselect.com/135585
Our Lab EMBS's Publication In collaboration with NIPER , Hyderabad, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/29053759
Our Lab EMBS's Publication In collaboration with Alagappa University, Tamil Nadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30950335
Our Lab EMBS's Publication In collaboration with Jawaharlal Nehru Technological University, Hyderabad , India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/28472910
Our Lab EMBS's Publication In collaboration with C.S.I.R – CRISAT, Karaikudi, Tamil Nadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30237676
Our Lab EMBS's Publication In collaboration with Karpagam academy of higher education, Eachinary, Coimbatore , Tamil Nadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30237672
Our Lab EMBS's Publication In collaboration with Ballets Olaeta Kalea, 4, 48014 Bilbao, Bizkaia, Spain. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/29199918
Our Lab EMBS's Publication In collaboration with Hospital for Genetic Diseases, Osmania University, Hyderabad - 500 016, Telangana, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/28472910
Our Lab EMBS's Publication In collaboration with School of Ocean Science and Technology, Kerala University of Fisheries and Ocean Studies, Panangad-682 506, Cochin, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27964704
Our Lab EMBS's Publication In collaboration with CODEWEL Nireekshana-ACET, Hyderabad, Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/26770024
Our Lab EMBS's Publication In collaboration with Bharathiyar University, Coimbatore-641046, Tamilnadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27919211
Our Lab EMBS's Publication In collaboration with LPU University, Phagwara, Punjab, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/31030499
Our Lab EMBS's Publication In collaboration with Department of Bioinformatics, Kerala University, Kerala. Publication Link: http://www.eurekaselect.com/135585
Our Lab EMBS's Publication In collaboration with Gandhi Medical College and Osmania Medical College, Hyderabad 500 038, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27450915
Our Lab EMBS's Publication In collaboration with National College (Affiliated to Bharathidasan University), Tiruchirapalli, 620 001 Tamil Nadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27266485
Our Lab EMBS's Publication In collaboration with University of Calicut - 673635, Kerala, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/23030611
Our Lab EMBS's Publication In collaboration with NIPER, Hyderabad, India. ) Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/29053759
Our Lab EMBS's Publication In collaboration with King George's Medical University, (Erstwhile C.S.M. Medical University), Lucknow-226 003, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/25579575
Our Lab EMBS's Publication In collaboration with School of Chemical & Biotechnology, SASTRA University, Thanjavur, India Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/25579569
Our Lab EMBS's Publication In collaboration with Safi center for scientific research, Malappuram, Kerala, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30237672
Our Lab EMBS's Publication In collaboration with Dept of Genetics, Osmania University, Hyderabad Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/25248957
Our Lab EMBS's Publication In collaboration with Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Hyderabad Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/26229292
Sincerely,
Dr. Anuraj Nayarisseri
Principal Scientist & Director,
Eminent Biosciences.
Mob :+91 97522 95342
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We, at Ethiopian Biotechnology Institute, are planning to have a mini-server to be used for storing omics data generated locally/or as collaborative efforts and for bioinformatics analyses. Currently, we do not have computer expert in order to help us list materials required for the development of the server. I appreciate any help related to the list of the requirements, what to install on the machine, how to integrate the machines to boost storage capacity and running speed, how to manage the system after installation, etc?
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Please have look on our(Eminent Biosciences (EMBS)) collaborations.. and let me know if interested to associate with us
Our recent publications In collaborations with industries and academia in India and world wide.
Our Lab EMBS's Publication In collaboration with Universidad Tecnológica Metropolitana, Santiago, Chile. Publication Link: https://pubmed.ncbi.nlm.nih.gov/33397265/
Our Lab EMBS's Publication In collaboration with Moscow State University , Russia. Publication Link: https://pubmed.ncbi.nlm.nih.gov/32967475/
Our Lab EMBS's Publication In collaboration with Icahn Institute of Genomics and Multiscale Biology,, Mount Sinai Health System, Manhattan, NY, USA. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/29199918
Our Lab EMBS's Publication In collaboration with University of Missouri, St. Louis, MO, USA. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30457050
Our Lab EMBS's Publication In collaboration with Virginia Commonwealth University, Richmond, Virginia, USA. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27852211
Our Lab EMBS's Publication In collaboration with ICMR- NIN(National Institute of Nutrition), Hyderabad Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/23030611
Our Lab EMBS's Publication In collaboration with University of Minnesota Duluth, Duluth MN 55811 USA. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27852211
Our Lab EMBS's Publication In collaboration with University of Yaounde I, PO Box 812, Yaoundé, Cameroon. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30950335
Our Lab EMBS's Publication In collaboration with Federal University of Paraíba, João Pessoa, PB, Brazil. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30693065
Our Lab EMBS's Publication In collaboration with collaboration with University of Yaoundé I, Yaoundé, Cameroon. Publication Link: https://pubmed.ncbi.nlm.nih.gov/31210847/
Our Lab EMBS's Publication In collaboration with University of the Basque Country UPV/EHU, 48080, Leioa, Spain. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27852204
Our Lab EMBS's Publication In collaboration with King Saud University, Riyadh, Saudi Arabia. Publication Link: http://www.eurekaselect.com/135585
Our Lab EMBS's Publication In collaboration with NIPER , Hyderabad, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/29053759
Our Lab EMBS's Publication In collaboration with Alagappa University, Tamil Nadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30950335
Our Lab EMBS's Publication In collaboration with Jawaharlal Nehru Technological University, Hyderabad , India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/28472910
Our Lab EMBS's Publication In collaboration with C.S.I.R – CRISAT, Karaikudi, Tamil Nadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30237676
Our Lab EMBS's Publication In collaboration with Karpagam academy of higher education, Eachinary, Coimbatore , Tamil Nadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30237672
Our Lab EMBS's Publication In collaboration with Ballets Olaeta Kalea, 4, 48014 Bilbao, Bizkaia, Spain. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/29199918
Our Lab EMBS's Publication In collaboration with Hospital for Genetic Diseases, Osmania University, Hyderabad - 500 016, Telangana, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/28472910
Our Lab EMBS's Publication In collaboration with School of Ocean Science and Technology, Kerala University of Fisheries and Ocean Studies, Panangad-682 506, Cochin, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27964704
Our Lab EMBS's Publication In collaboration with CODEWEL Nireekshana-ACET, Hyderabad, Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/26770024
Our Lab EMBS's Publication In collaboration with Bharathiyar University, Coimbatore-641046, Tamilnadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27919211
Our Lab EMBS's Publication In collaboration with LPU University, Phagwara, Punjab, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/31030499
Our Lab EMBS's Publication In collaboration with Department of Bioinformatics, Kerala University, Kerala. Publication Link: http://www.eurekaselect.com/135585
Our Lab EMBS's Publication In collaboration with Gandhi Medical College and Osmania Medical College, Hyderabad 500 038, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27450915
Our Lab EMBS's Publication In collaboration with National College (Affiliated to Bharathidasan University), Tiruchirapalli, 620 001 Tamil Nadu, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/27266485
Our Lab EMBS's Publication In collaboration with University of Calicut - 673635, Kerala, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/23030611
Our Lab EMBS's Publication In collaboration with NIPER, Hyderabad, India. ) Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/29053759
Our Lab EMBS's Publication In collaboration with King George's Medical University, (Erstwhile C.S.M. Medical University), Lucknow-226 003, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/25579575
Our Lab EMBS's Publication In collaboration with School of Chemical & Biotechnology, SASTRA University, Thanjavur, India Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/25579569
Our Lab EMBS's Publication In collaboration with Safi center for scientific research, Malappuram, Kerala, India. Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/30237672
Our Lab EMBS's Publication In collaboration with Dept of Genetics, Osmania University, Hyderabad Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/25248957
Our Lab EMBS's Publication In collaboration with Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Hyderabad Publication Link: https://www.ncbi.nlm.nih.gov/pubmed/26229292
Sincerely,
Dr. Anuraj Nayarisseri
Principal Scientist & Director,
Eminent Biosciences.
Mob :+91 97522 95342
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Demographers estimate that by 2050, the number of people on Earth will reach 10 billion. With such a number of people, the agricultural economy, logistics of food supplies and people's eating habits will have to change. It is likely that economics will force these processes, which will result in the transition of the majority of humanity to nutrition mainly based on vegetable and vegetarian diets. Meat production is many times more expensive than the production of cereals, fruits and vegetables. In addition, according to scientific research and the theory of futurologists, the production of traditional meat, e.g. pork and beef, may be replaced by the production of protein from insect breeding. Research shows that there are more proteins in the bodies of insects than in traditional meat dishes. In addition, the logistics of food supplies, agri-food products will have to improve. Systems for matching agricultural and reptile production to the current needs of the industry and the nutritional needs of people will be improved so as to reduce the scale of food wastage. The biggest threat to the implementation of this plan may be unexpected atmospheric phenomena, natural disasters, droughts, hurricanes, tropical heat in the areas in which agricultural crops have been cultivated so far. In addition, industrial exploitation of arable land and climate change causes soil depletion and the disintegration of areas suitable for agricultural production. Therefore, it will be necessary to continue the technological progress in the production of crops, in biotechnology, in the creation of new plant varieties resistant to pests and adverse climatic changes.
Please, answer, comments. I invite you to the discussion.
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With rising income food consumption patterns also change. Calorie intakes of poor and rich people are surprisingly similar, but rich people consume more protein. This adds about a further 1 percent growth to food demand which means that the world will need to produce approximately two percent more food annually if today’s poor become rich. The growth of supply needed for the future about 2 percent annually has to come mainly from available farmland to avoid an overly negative impact on fragile ecosystems. This requires finance, investments, innovation, and knowledge to improve the yields at existing farmlands. The yield gap between what’s needed and what’s being produced is still very high. On the other hand, reducing food waste can have a significant impact on the availability of food. Reducing food waste can improve the efficiency of food value chains and help to distribute food more evenly to those in need.
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Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target mutations. and now I would like to know how can I design a specific gRNA for it?
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Reality may increasingly hurt – #Genomic #targetedtherapy
Changes between 2006 and 2020
-eligibility from 5.13% to 13.60% *only*
-response from 2.73% to 7.04% *only*
The authors use in their discussion different paraphrasing: “the percentage of eligibility for and response to genome-targeted drugs increased 54% and 28%, respectively
which from the objective point of view is correct, but this implies a major improvement, although the reality of inefficiency is different
Some may state *the house of cards collapse of the breakthrough* ?
Otherwise today's basics of biotechnological power politics in created hypes of landmarks, breakthroughs, which might be seen in the accordance of the Latin epic poem, The Aeneid by Virgil:
parcere subiectis, et debellare superbos’ (I believe it at any rate)
References
Article Haslam et al. (2021) Ann Oncol 2021, DOI 10.1016/j.annonc.2021.04.003
The Aenedid from Virgil Vergil (19 BC), Aeneis, Liber sextus, Vers 847-853.
#medicine #health #medicalsciences #breakthroughs #landmarks #hallmark #hype #criticalthinking
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Dear Pr Brücher,
Thank you for sharing with us these useful informations.
Best regards,
Pr Hambaba
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Common fragile sites (CFSs) are large chromosomal regions that exhibit breakage on metaphase chromosomes upon replication stress. As a result, they become preferentially unstable at the early stage of cancer development and are hotspots for chromosomal rearrangements in cancers.
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I prepared a 24:1 mixture of chloroform and isoamyl alcohol to make 100 ml solution. So I added the 24 ml chloroform and 1ml isoamyl alcohol and together to them added the vol 75ml with dd water. What can be the reson for the two layers formed and troubleshooting for it !!
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Hi,
you should not add any water to the preparation. The two phases are formed by the organic (chloroform) and aqueous (water) liquids. Thus use only 24ml chloroform and 1ml isoamyl alcohol.
Best,
Norman
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Greetings,
I have more than 100 bacterial isolates, and I want to identify them. Would you like to suggest to be the best way for their 16s rRNA identification (most probably by 27F and 1492R universal primers), concerning; 1) reliability, 2)time taken, 3)cost/price, and 4)service provider from KSA.
Thanks.
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You can classify your bacteria up to a certain level by various microbial and biochemical techniques. But to identify the bacteria to the genus and species level, you need to do 16S rRNA gene sequencing. You can try sanger dideoxy sequencing method. If you are using applied bio-systems sequencer, You will get 2 trace files .abi1 and .abi2(fwd and reverse) files. You may use DNA baser software to assemble both the forward and reverse trace files. After assembly you will get the contigs sequence in fasta format and then perform a nucleic acid blastn against 16S ribosomal RNA sequences(Bacteria and Archea). You can identify your bacteria.
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I have made a fluorescent protein biosensor that is sensitive to temperature. I will need to measure the signal at 30 degrees by flow cytometer. But normally, samples were run at room Tem. And the sheath fluid is also at room Tem. How can I measure the biosensor by flow cytometer at tem higher than room tem? Someone told me that the time from sample to the detectors is within mili seconds so it should be fine but I am not sure about that. Anyone has any suggestion?
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Sorry, these questions are not my specialty
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Hi, everyone!
I'm looking for an untouched area or highly emerged problem that humans (probably farmers, pharmaceutical industry-related) facing recently or might be faced in the near future which can be sorted out by marine microbiological or biotechnological resources.
or please suggest to me any resource related to this matter. your valuable comments are highly appriciated.
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Can turmeric be used as a substitute for the other comercially availble antifungal agents during plant tissue culture?
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I have not had the experience of using turmeric as an antimicrobial agent in PTC. In our country, this substance is much more expensive than conventional disinfectants. On the other hand, we know that turmeric has antimicrobial properties, but it is a natural phenolic compound and its negative effect on cell growth and proliferation has been reported. Also, in my opinion, since this yellow substance reduces the transparency of the culture medium and the environment becomes opaque, I preffer to use the transparent materials.
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I have used wild type E. Coli BL21 DE3 (non-transformed cell) as control for my fluorescence experiment and measured the fluorescence value in a spectrofluorophotometer. As time increases, control fluorescence value also increased along with growth of the cell. I am curious to know how come the wild type E. Coli BL21 DE3 generates value in spectrofluorophotometer as it does not harbor any plasmid? Kindly help me to overcome the control fluorescence.
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This phenomenon has been nicely quantified here https://hal.archives-ouvertes.fr/hal-01628395/document
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Hello everyone and thank you for reading.
For a student research project, I need data/information concerning the environmental impacts of sulphur and CO2 removal technologies used in biogas plants. The technologies analyzed in our paper are absorption in water, chemical absorption using NaOH/FeCl3/Fe(OH)3, adsorption with activated carbon, membranes and biotechnological approaches (oxygen dosing, biotrickling filter, bioscrubber).
As this description is very broad and our paper should just give an overview, I would be very thankful for any sources.
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Hi guys. This is a great topic to discuss, in that way, to answer this question, I suggest reading the following articles:
and...
Let me know if they were helpful to you folks doing a recommendation and citation of them.
Best Regards.
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Phylogenetic analysis after 16-S r-RNA amplification many times indicate discovery of new bacterial species. What are the steps involved in confirming discovery of a new bacterial species and naming it ?
S S Maitra , associate professor , School of Biotechnology , JNU,.
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Dear Subhrangsu Maitra,
You can import the nucleotide sequence in NCBI (https://www.ncbi.nlm.nih.gov/) and blast it. The results of blasting indicates the sequence identity of the new microorganism. If it is a new microorganism, it will be identified by NCBI.
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Biosynthesis of nano particles by bacteria is a competitive advantage with which they can kill other bacteria.But why the nano particles that is biosynthesis is not toxic for the host bacteria ?
I want to know how host bacteria protect themselves?
How does the host bacterium detoxify the biosynthetic nano particles?
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I am working on the strain Penicillium chrysogenum, but I have a problem with cultivation in a liquid medium and it doesn't grow or its growth is not properly. As we have a general incubator (33c and 162rpm) I use this incubator for the project. we have also an incubator that has not shaker but I can change its temperature. I prepare the culture medium that is attached under this question and I pour it into a flask and autoclave it at 121 c (all of the materials in one flask). I use solid culture as inoculum (one loop). 33c and 162rpm.
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You can change the medium that you are using for growing this fungus.
I think that PDA medium is very suitable for growing this fungus.
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Vitis Vinifera : Transcription factor proteins NAC1, NAC08, NAC17, NAC26 already showed a resistance against abiotic and biotic stresses via different articles.
My Vitis Vinifera NAC36 gene might have same function like them ? What the best way to start with this comparison ?? and is it possible ??
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Hi everyone,
Do anyone have experience in detecting MMP9 expression? I want to detect MMP9 expression in plant extract-treated HCT116 cells, I used antibodies from Santa Cruz Biotech, diluted 1:1000. I got two bands at around 22 kDa, 45 kDa. But in my reference, MMP9 will appear at 92 kDa. Could anyone help me to explain why I get these bands? Thank you very much for your help.
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CRISPR-Cas13 can be used for the Detection and Treatment of COVID-19. Is CRISPR-Cas13 more advanced technology than CRISPR-Cas9? Will be the CRISPR-Cas13 cancel the CRISPR-Cas9 ? Is CRISPR-Cas13 a more safe method than CRISPR-Cas9?
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Sometimes all of scientific articles are not available in google search even in another search engine. But when I intended to do a new work, so how can I know that this is first time. Even though, it may be that the work has been done before but it did not appear in my search engine. So, what could basis that we can say this is first time work or research.
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Mirza Mienur Meher sure I agree with all comments about search details: databases (Google Scholar is apparently the most comprehensive and free), keywords, reading recent reviewers, etc. But the truth is that you cannot confirm by 100% the novelty of your research. Always there is a chance of unintentional plagiarism. It is NOT a crime because every real research contains something very individual/personal and original. A thoughtless work in science can be 100% plagiarism, but thoughtful creative research - never! More than 99% of published papers are not absolutely novel, it is OK to have just a few novel elements. More important is the confirmation that your data agree with already available knowledge. It means that you must repeat research done previously rather than avoid it!
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Dear RG members, kindly share your thoughts.
Although both Oxford-AstraZeneca's Covishield vaccine and the indigenously-developed Covaxin of Bharat Biotech are given approval by Drugs Controller General of India (DCGI) back in January 2021, still majority people are preferring Covishield vaccine over Covaxin.
Is this because of the Types of the Vaccines (Viral vector (Covishield), Inactivated (Covaxin) etc.) and their corresponding effectiveness? Or there are some other issues e.g., severe side effects and less antibody development against newer variants/strains?
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India’s Bharat Biotech Says Vaccine Is Effective Against Delta Variant. Company said its vaccine demonstrated an overall efficacy of 77.8% in Phase 3 clinical trials https://www.google.com/amp/s/www.wsj.com/amp/articles/indias-bharat-biotech-says-vaccine-is-effective-against-delta-variant-11625325879
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We are using pJET1.2 vector (Thermo CLoneJET PCR Cloning kit) for cloning 1400bp fragment. We have blunted our insert following kit protocol. According to kit self ligated vector should not grow on plate due to lethal gene. But we are getting 8 out of 10 colonies to be false ligated. Can anyone please tell what can be the possible reason? We have used insert:vector in 3:1 ratio. Gel image after colony PCR is attached.
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It’s 2021 And we have started using this cloning kit. Initially we had problems like multiple bands appearing in the cloned products. However this was solved with increasing the ligation time. Now the problem is that, it works completely fine with smaller sized inserts (below 1kb) but not with larger ones. We are still getting multiple bands.
can any one suggest me?
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Hi Everyone
Could you please name journals which publish review article related to Algae Biotechnology for free within less duration.
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You can try Trends in Biotechnology (IF: 14.343)
Thanks
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