Questions related to Biotechnology
I am a student of biotechnology and an independent SARS CoV-2 researcher from India. For finding the academia research status and analysis of the integration of innovation with research, I have created a set of Multiple choice type questions about your experience as a researcher. The google form requires nothing but your honesty and openness for research. Feel free to ask questions and DM. The questions will assist in gauging the level of innovation and writing in academia.
If possible, please do forward this little form to your fellow researchers and other amazing scientists. I would be highly grateful.
Long story short, I need to degrade 30ug of RNA and i need to do it at 4C. I want to use only as much RNAse A as necessary.
So if performing the reaction at 4C, how long of incubation time and how much RNAse A would i need to degrade 30ug of RNA?
For example, would 1ug of RNAse A(20ug/ml conc.) for 15min at 4C be enough?
Dear colleagues and respected seniors, I am searching for a biochemical reaction to identify the km and vmax values for the said enzyme, which converts alpha carotene into Lutein, but still I am not able to identify, so if anyone could help to find it, give me a reference article/ paper please, I will be very thankful to al of you.
Thanks and best regards
Mr. Rafi Ullah Khan
PhD candidate (Biotechnology)
Two months ago, I bought pyrocystis fusiformis culture for my research. Unfortunately, no bioluminescence was observed, and the culture seemed to be deficient from the start.
Now I'm trying to buy a Pyrocystis fusiformis culture again and found a lab called PyroFarms, but before I order, I wanted to check if anyone had any past experience with them. or alternatively, if you could name any other trustworthy sources where I could buy a P. fusiformis culture, I would be grateful.
As all the world is busy in discovering cure for COVID-19, so why not radiation? COVID-19 has been reported to effect the human's lungs and liver (specific tissues), So, what if those parts are exposed to radiation for the rapid recovery of the patients? Is it possible? Or, is there any possibility for radiotherapy of COVID-19?
I couldn't see much paper where plant breeders use biochemical such as proline content Malondialdehyde (MDA) and dyes such as NBT or DAB( for ROS detection) for screening stress-tolerant accession on a large scale (100-200 which I suppose is possible to do). Are not these methods better than phenotyping grain yield, biomass, plant height, NDVI, LAI, etc?
I am Janu Newar, Ph.D. Scholar from School of Biotechnology, KIIT University, Bhubaneswar, Odisha. Our lab is basically working on proteins from biological sources. This work involves the identification of some proteins. I would like to request some help with the Agilent 6530 Q-TOF instrument.
For identification of the protein, we are using a glass insert of 0.2ml volume (Part no: 5188-5390). My query is can we use 0.025ml of the sample volume in this insert and can inject 0.02ml of sample in the column. If not, then how much lower volume can we use in this 0.2ml insert?
I have 100 uM concentration of ssDNA-FQ with molecular weight ~ 2400, and100 uM of 6-FAM with a molecular weight ~376. While performing a fluorescence assay, I get higher fluorescence intensity with cleaved ssDNA-FQ than 6-FAM. I am not sure that with the same concentration of ssDNA-FQ and 6-FAM whether I have the same number of molecules or not. Has anybody experienced anything like this before? Please let me know. Thanks in advance.
I want to do dialysis purification for the cyclic peptide 2K after its modification using membrane dialysis MWCO 1K but I am worry that the cyclic structure has a smaller size than its linear one and ca pass through the membrane.Is the cyclic structure has bigger or smaller size compared with the linear structure?
Direct experiences with Biotechnological and Biomedical Approach? Therapies, long-term implications?
I'm not looking for definitions but protocols and methods that you personally have made and put into practice. Any information you have is welcome.
By using this cytokine kit I am getting high absorbance value in my positive controls. Recommended values for positive controls are 1.5-2.5, but I am getting values around 3.0-3.5.
In some of my negative control I have absorbance more than the experimental sample. Eg. negative control value 0.04 and sample value 0.01.
I am planning a fluid dynamic experiment and wonder what tubing material is biocompatible for cell culturing experiment. Also wonder if there is a tubing material that can be sterilize.
What are the limitations and disadvantages of Real-Time PCR (RT-PCR)?
What is a more specific and sensitive technique that can be used in the laboratory instead, particularly in cancer diagnosis?
Novozyme 234 is the best enzyme for the isolation of protoplast but in recent days the Novozyme 234 are not commercially available. So is there any other method (enzymatic) for this isolation?
Dear scientists and researchers. I ask those who have knowledge of the names of Free of charge, indexed scientific journals in the field of biotechnology; gives us links to those Journals.
The bio-technologies today are becoming important part of the industry and science research development and innovation. In case of brain tumor or injuries, apart from surgical intervention, the data may also be collected via noninvasive bio-signals such as EEG, transmitted, stored and analysed in e-Health Electronic Health Record. What are future research directions in bio-technology driven bio computing?
Probiotics can be used as dietary supplements or drugs to treat diseases. The genetically modified probiotics will offer much more beneficial features than the wild type probiotics. Does FDA approve genetically modified probiotics? When do you think they will approve? What scientific questions do we need to resolve before FDA can approve? Can we use genetically modified probiotics as dietary supplements without FDA approval?
I am re-writing now my previous question.
Are you aware if there is any protease with very low efficiency to cleavage IgG ?
Are you aware of any publication?
Our quantum dot(g-C3N4/CQDs) was prepared from melamine and then exposed to phenylbronic acid(PBA) for the next steps of the experiment - whose fluorescent properties were quenched (Figure 1) but after 5 days and quantum dot solution dialysis with PBA (1000D) A little of its fluorescent property back, what is your analysis?
Is there a scientific journal concerned with biology, microbiology, biotechnologies or medicinal plants, issued monthly or every two months, and is ifree of charge?
Is there an accredited scientific journal concerned with biology, microbiology, biotechnologies or medicinal plants, issued monthly or every two months, and is it free of charge?
I am trying to get CRISPR/Cas9 working in my lab. I have a strain of Aspergillus that expresses the Cas9, and I need a way of introducing the gRNA.
When we are focusing on our interest in the field is obvious to affect the GPA but is said that the GPA is prime for the selection characteristics for foreign study does it really matters or it is a myth?
I dissolved indomethacin (Santa Cruz Biotech - SC-200503) in DMSO and prepared stock of 25 mg/mL
In my study, I inject 10 mg/kg i.p., injection volume 0,5 mL and I work with 250-300 g rats.
When I diluated the stock solution with saline (%0,9 NaCl), the diluated solution has been crystallized.
How can I avoid that?
Open for any suggestions.
My kind regards.
I want to remove thiol molecules on a gold substrate. After searching, I found couple of method described below;
1. NH4OH–H2O2–H2O solution aim is to initiate the oxidation of the thiol compound
2. Thermal desorption (above 200 ◦C)
3. Ultraviolet light and ozone (O3)
Do you have any experience about these methods. If your answer is yes, can you share your experience with us? Thank you for sharing and helping,
I read several papers where researchers use several strains of the same species for analysis of recombination or selection pressure however there are few papers in which researchers use the multiple species belonging to one 'Genus', not the multiple strains that belong to one 'Species'. What difference is expected in the result and how it can be interpreted.
I want to know how my pathogenic organism 'Xyz abcde' is evolved (recombination and selection pressure). 5 different strains of 'Xyz abcde' is sequenced while 9 organisms in Genus 'Xyz' is sequenced.
Which dataset I should use to proceed and why? How it will make difference in the analysis?
Genomic and biotechnological studies, as mentioned, using RNA polymerase and RNA are preferred over DNA studies (using DNA and DNA polymerase II) because the RNA polymerase does not need a primer(?) Would a primerless DNA polymerase II not need an an RNA primer.(?)
crRNA is responsible for recognizing and binding the sequences next to protospacer-adjacent motif (PAM), NGG, on the target DNA, whereas tracrRNA is essential to maintain cas9 nuclease activity. and most of the miRNA does not contain the PAM sequence (5’-NGG-3) 4. so how can you target them by using CRISPR Cas system?
Metabolic rewiring and epigenetic remodeling, which are closely linked and reciprocally regulate each other, are among the well-known cancer hallmarks. Studies have reported use of Onco-metabolites to metabolically reprogram the epigenetic of cancer. I was wondering what might be major limitations of such techniques?
in cancer therapy, sometimes you have to target multi-targets to understand the molecular pathway of the specific protein. CHyMErA can target multi targets but it is not safe and has a high risk of extra mutation!
I am working on a Plastic Bioremediation Project and need to pre-treat the plastic waste samples before further work can be done.
Disclaimer: I am not a microbiologist by profession or training and this is a curious/knowledge question
I have read some old articles that suggest the inoculum concentration to be 10^5 according to EUCAST.
I have also read some review articles suggesting 10^8 (0.5McFarland standard)- citing CLSI.
I am not able to download CLSI guidelines, can anyone help me get a copy to read about broth dilution and initial cell density actually prescribed?
Please let me know if these standards have changed? What cell concentration do researchers commonly use to read MIC (in North America or elsewhere)
Hi there everyone,
I'd like to take your opinion from your experience and observation. I'm currently looking to advance my career. I work as a research assistant/technologist in Molecular Medicine and have an MSc in Biotechnology and BSc in Pharmaceutical Sciences.
What options are there (apart from PhD), to develop my career, and not always be an RA?
Thank you :)
I have a task which I need to recover rhamnolipid by using solvent extraction. I have gone through some papers but some of them using different solvent for rhamnolipid recovery, which are 1) methanol: chloroform: acetone (1:1:1 v/v) and 2) methanol: chloroform (2:1 v/v). Do they affect the rhamnolipid yield? Which solvent should I use?
Transfections using chemical transfection reagents rely on electrostatic interactions to bind with nucleic acids and to target cell membranes. what is the best and efficient transfection reagent?
For example, DNA has functional groups such as methyl groups and nanoparticles can interact with them and alter them. I was wondering if we have any technique by which we can quantify them or maybe do a qualitative analysis. Any leads would be great, Thank :)
I have to measure the concentration of a specific protein inside the blood. And Unfortunately, our lab does not have enough facilities to perform western blotting, so can we use ELISA instead?
What is the best way or techniques to have accurate results????
Can ELISA be used instead of Western-Blotting to measure protein concentration?
Good research is based on good relationship between the mentor or supervisor and the scholar. What are the qualities a supervisor or mentor must have to have a healthy and friendly environment in the laboratory?
So my last year project is Drug Efflux Pumps and Persistence in Methicillin Resistant Staphylococcus aureus and we gonna focus on persister cells to study the path way of antimicrobial resistance...my question is how can i link bioinformatics and some coding to this project without requiring wgs cause it's not an option inside our lab !I need a small yet beneficial technique/ tools in small scale that i can learn and implement by my self .PS I love programming in general but im still new to bioinformatics so i need help to link my passion for coding and my field "biotechnology"
We, at Ethiopian Biotechnology Institute, are planning to have a mini-server to be used for storing omics data generated locally/or as collaborative efforts and for bioinformatics analyses. Currently, we do not have computer expert in order to help us list materials required for the development of the server. I appreciate any help related to the list of the requirements, what to install on the machine, how to integrate the machines to boost storage capacity and running speed, how to manage the system after installation, etc?
Demographers estimate that by 2050, the number of people on Earth will reach 10 billion. With such a number of people, the agricultural economy, logistics of food supplies and people's eating habits will have to change. It is likely that economics will force these processes, which will result in the transition of the majority of humanity to nutrition mainly based on vegetable and vegetarian diets. Meat production is many times more expensive than the production of cereals, fruits and vegetables. In addition, according to scientific research and the theory of futurologists, the production of traditional meat, e.g. pork and beef, may be replaced by the production of protein from insect breeding. Research shows that there are more proteins in the bodies of insects than in traditional meat dishes. In addition, the logistics of food supplies, agri-food products will have to improve. Systems for matching agricultural and reptile production to the current needs of the industry and the nutritional needs of people will be improved so as to reduce the scale of food wastage. The biggest threat to the implementation of this plan may be unexpected atmospheric phenomena, natural disasters, droughts, hurricanes, tropical heat in the areas in which agricultural crops have been cultivated so far. In addition, industrial exploitation of arable land and climate change causes soil depletion and the disintegration of areas suitable for agricultural production. Therefore, it will be necessary to continue the technological progress in the production of crops, in biotechnology, in the creation of new plant varieties resistant to pests and adverse climatic changes.
Please, answer, comments. I invite you to the discussion.
Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target mutations. and now I would like to know how can I design a specific gRNA for it?
Reality may increasingly hurt – #Genomic #targetedtherapy
Changes between 2006 and 2020
-eligibility from 5.13% to 13.60% *only*
-response from 2.73% to 7.04% *only*
The authors use in their discussion different paraphrasing: “the percentage of eligibility for and response to genome-targeted drugs increased 54% and 28%, respectively”
which from the objective point of view is correct, but this implies a major improvement, although the reality of inefficiency is different
Some may state *the house of cards collapse of the breakthrough* ?
Otherwise today's basics of biotechnological power politics in created hypes of landmarks, breakthroughs, which might be seen in the accordance of the Latin epic poem, The Aeneid by Virgil:
‘parcere subiectis, et debellare superbos’ (I believe it at any rate)
Article Haslam et al. (2021) Ann Oncol 2021, DOI 10.1016/j.annonc.2021.04.003
The Aenedid from Virgil Vergil (19 BC), Aeneis, Liber sextus, Vers 847-853.
#medicine #health #medicalsciences #breakthroughs #landmarks #hallmark #hype #criticalthinking
Common fragile sites (CFSs) are large chromosomal regions that exhibit breakage on metaphase chromosomes upon replication stress. As a result, they become preferentially unstable at the early stage of cancer development and are hotspots for chromosomal rearrangements in cancers.
I prepared a 24:1 mixture of chloroform and isoamyl alcohol to make 100 ml solution. So I added the 24 ml chloroform and 1ml isoamyl alcohol and together to them added the vol 75ml with dd water. What can be the reson for the two layers formed and troubleshooting for it !!
I have more than 100 bacterial isolates, and I want to identify them. Would you like to suggest to be the best way for their 16s rRNA identification (most probably by 27F and 1492R universal primers), concerning; 1) reliability, 2)time taken, 3)cost/price, and 4)service provider from KSA.
I have made a fluorescent protein biosensor that is sensitive to temperature. I will need to measure the signal at 30 degrees by flow cytometer. But normally, samples were run at room Tem. And the sheath fluid is also at room Tem. How can I measure the biosensor by flow cytometer at tem higher than room tem? Someone told me that the time from sample to the detectors is within mili seconds so it should be fine but I am not sure about that. Anyone has any suggestion?
I'm looking for an untouched area or highly emerged problem that humans (probably farmers, pharmaceutical industry-related) facing recently or might be faced in the near future which can be sorted out by marine microbiological or biotechnological resources.
or please suggest to me any resource related to this matter. your valuable comments are highly appriciated.
Can turmeric be used as a substitute for the other comercially availble antifungal agents during plant tissue culture?
I have used wild type E. Coli BL21 DE3 (non-transformed cell) as control for my fluorescence experiment and measured the fluorescence value in a spectrofluorophotometer. As time increases, control fluorescence value also increased along with growth of the cell. I am curious to know how come the wild type E. Coli BL21 DE3 generates value in spectrofluorophotometer as it does not harbor any plasmid? Kindly help me to overcome the control fluorescence.
Hello everyone and thank you for reading.
For a student research project, I need data/information concerning the environmental impacts of sulphur and CO2 removal technologies used in biogas plants. The technologies analyzed in our paper are absorption in water, chemical absorption using NaOH/FeCl3/Fe(OH)3, adsorption with activated carbon, membranes and biotechnological approaches (oxygen dosing, biotrickling filter, bioscrubber).
As this description is very broad and our paper should just give an overview, I would be very thankful for any sources.
Phylogenetic analysis after 16-S r-RNA amplification many times indicate discovery of new bacterial species. What are the steps involved in confirming discovery of a new bacterial species and naming it ?
S S Maitra , associate professor , School of Biotechnology , JNU,.
Biosynthesis of nano particles by bacteria is a competitive advantage with which they can kill other bacteria.But why the nano particles that is biosynthesis is not toxic for the host bacteria ?
I want to know how host bacteria protect themselves?
How does the host bacterium detoxify the biosynthetic nano particles?
I am working on the strain Penicillium chrysogenum, but I have a problem with cultivation in a liquid medium and it doesn't grow or its growth is not properly. As we have a general incubator (33c and 162rpm) I use this incubator for the project. we have also an incubator that has not shaker but I can change its temperature. I prepare the culture medium that is attached under this question and I pour it into a flask and autoclave it at 121 c (all of the materials in one flask). I use solid culture as inoculum (one loop). 33c and 162rpm.
Vitis Vinifera : Transcription factor proteins NAC1, NAC08, NAC17, NAC26 already showed a resistance against abiotic and biotic stresses via different articles.
My Vitis Vinifera NAC36 gene might have same function like them ? What the best way to start with this comparison ?? and is it possible ??
Do anyone have experience in detecting MMP9 expression? I want to detect MMP9 expression in plant extract-treated HCT116 cells, I used antibodies from Santa Cruz Biotech, diluted 1:1000. I got two bands at around 22 kDa, 45 kDa. But in my reference, MMP9 will appear at 92 kDa. Could anyone help me to explain why I get these bands? Thank you very much for your help.
Sometimes all of scientific articles are not available in google search even in another search engine. But when I intended to do a new work, so how can I know that this is first time. Even though, it may be that the work has been done before but it did not appear in my search engine. So, what could basis that we can say this is first time work or research.
Dear RG members, kindly share your thoughts.
Although both Oxford-AstraZeneca's Covishield vaccine and the indigenously-developed Covaxin of Bharat Biotech are given approval by Drugs Controller General of India (DCGI) back in January 2021, still majority people are preferring Covishield vaccine over Covaxin.
Is this because of the Types of the Vaccines (Viral vector (Covishield), Inactivated (Covaxin) etc.) and their corresponding effectiveness? Or there are some other issues e.g., severe side effects and less antibody development against newer variants/strains?
We are using pJET1.2 vector (Thermo CLoneJET PCR Cloning kit) for cloning 1400bp fragment. We have blunted our insert following kit protocol. According to kit self ligated vector should not grow on plate due to lethal gene. But we are getting 8 out of 10 colonies to be false ligated. Can anyone please tell what can be the possible reason? We have used insert:vector in 3:1 ratio. Gel image after colony PCR is attached.