Questions related to Biosensors
i have uploaded image about question.i want to design some biosensor in electrochemistry. i need two chemical molecules (A & B). A is conjugated on a surface by some linker and B is connected to a electrode surface. when the linker breaks, A and B should joint each other especially with targeted linkage. A should have a property to induce electric current in B.
please guide me which molecules can be A and B?
I want to determine the glucose concentration of my samples using the Biosensor. However, my samples contain glucose oxidase (GOD) in order to remove hydrogen peroxide from my samples.
The principle of the biosensor is that the glucose in the sample generates hydrogen peroxide by glucose oxidase in the enzyme electrode. Then, the generated hydrogen peroxide is oxidized by the platinum anode. The glucose concentration is then determined by the change of the currect value according to this oxidation.
Because my samples contain glucose oxidase, the glucose concentration decreases.
I determined the glucose concentration of a known sample, having a concentration of 1.9 g/L. However, when GOD is added to my sample, the concentration decreases to 1.75 g/L .
Therefore, I want to remove glucose oxidase prior to using the Biosensor. How can I do this?
I am having some issues with Bovine serum I have purchased and am wondering if there is a better serum to purchase that others have had better success using? any advice would be appreciated.
I want to calculate the LOD for this sensor, based on the figures in this table.
I know that LOD is 3 x std LOB
But what is a method to decipher LOD from this data set?
I'm using COMSOL Multiphysics SW for the design of biosensor.
I want to add peizoresistive material for the sensing purpose. But there is no peizoresistive Material in materials library. So, I hope I can add it from outside. But I don't know how add.
Currently I am working on SPR-PCF biosensor for biochemical detection , i am stuck in comsol multiphysics to find the sensitivity of SPR-PCF sensor how to do the analysis where to put the formula and how to check the results in comsol please someone suggest me the solution and please give the detailed explaination for that
I had written two papers. One of the paper is on analog performance of GAA MOSFET and second one is on bio sensing performance of GAA MOSFET.
Both these papers are simulation based. I had sent them to various journals but unfortunately got rejected due to absence of any device physics( I am working on device physics in my current work-next paper).
I want to know if any Scopus or SCI based journal that can possibly accept these papers. I am really depressed since its been more than a year but its getting rejected. Any Scopus journal will also work but should be recognized.
Please, suggest me some journals seniors and respected people. Kindly help me.
DOMAIN- Electronics(VLSI) and MOSFET based Biosensors
In an ionic solution a charged surface also attracts counterions from the solution, forming an electrical double layer (EDL). This EDL effectively screens off the charge, and in physiologically relevant conditions, the characteristic charge screening length (Debye length) is less than a nanometer (nm). Thus, in high ionic strength solutions, charge based (DC) detection is fundamentally impeded. What are established solutions to overcome this problem?
A pH-responsive hydrogel was synthesized and dried. It swells at higher pH and shrinks at lower pH. How can this behavour be utilized in making a biosensing device?
Moreover, if superparamagnetic iron oxide nanoparticles are embedded in the pH-sensitive hydrogel, how can this material be used in making a biosensor?
I am working on a lateral flow rapid test using the sandwich method. I tried different parameters to get the result in the full test but no success. However, it works in the half test format.
When there is no sample and conjugate pad and I put the antigen and conjugate AuNPs in a well and run the half strip, it works and when I run the full strip it did not even show a faint line.
Any idea about what to do to solve the problem.
P.S. I want to use the strip for detecting the antigen in 100 ng/mL concentration.
Many biosensor schemes have been developed as an alternative to classical methods, please explain?
The unique physicochemical properties of CNTs make them among prime candidates for numerous applications in biomedical fields including drug delivery, gene therapy, biosensors, and tissue engineering applications.
We have designed a dual enzyme electrochemical biosensor that generates glucose at the end and ultimately to H2O2 to generate the signal with help of glucose oxidase. We would like to challenge the proposed biosensor in serum samples however, glucose that is already presented in those samples in high concentrations I suppose can interfere with the signal. Can someone suggest how to avoid the interference?
I am currently determining EIS spectra on different carbon-based electrodes. For this, I use a PalmsensPS4 at pretty standard conditions in a three electrode setup (0.1 Hz to 100 kHz) and measure against OCP in a 5mM [Fe(CN6)]3- / [Fe(CN6)]4- (1:1) in phosphate buffered saline (PBS) at pH 7. I then use EIS Spectrum analyser to fit the curves. One type of my electrodes results in data that can be easily fitted with a standard electrochemical Randles cell (yellow in figure). The other type, however, seems to have lower impedance overall, and very low charge-transfer resistance in particular, and I cannot seem to find a model that fits it (blue in figure). When I modify the electrodes with PEDOT, I get the same picture.
I have some questions relating to this:
(1) How can I explain, in EIS terms, what happened to these electrodes?
(2) Which model can I use to generate useful fits?
(3) Are there any papers in which these strange EIS curves have been obtained and are explained?
(4) Is it worth to change experimental conditions and what would you change to obtain meaningful data? I was planning to change the electrolyte concentration and / or the potential (though I am not sure if that would give any meaningful results).
I am trying to get an accurate measurement of the size of a commercial carboxyl coated QD, vendor states the size is between 15-20nm. The sample is in a Borate Buffer at pH9.0, the same buffer the QDs were packaged. The scan has an intensity distribution: 70+/-111.1nm; volume distribution: 2.6+/-2.1nm; number distribution: 1.5+/-0.5nm. I am wondering why the volume and number distribution are reading low and not closer to the 15-20nm range as the vendor states.
I am having some DNA probe conjugated on gel surface (polyacrylamide) and I want to perform reverse transcription and PCR after the DNA probe capture target RNA. Yet the volume of water absorbed in the gel cause a problem. How should I calculate and decide the amount of reaction mix to use? should I directly add same volume, 2X concentration onto the gel (20ul gel in a well)?
It is commonly used of silica membrae membrane in DNA extraction column to bind and release DNA. Yet I wonder can I simply use extraction tube to filter and collect ~20um beads (conjugated with DNA), then take out the whole membrane for the downstream reverse transcription, exonuclease and PCR. Will the membrane block or impede the enzyme to access the DNA on spheres? The membrane will be fully immersed in the reaction mix.
Is it necessary to do SAM before Fibronectin? With this modification, how long does it take for cells to spread on top of an electrode?
I have made a fluorescent protein biosensor that is sensitive to temperature. I will need to measure the signal at 30 degrees by flow cytometer. But normally, samples were run at room Tem. And the sheath fluid is also at room Tem. How can I measure the biosensor by flow cytometer at tem higher than room tem? Someone told me that the time from sample to the detectors is within mili seconds so it should be fine but I am not sure about that. Anyone has any suggestion?
Recently, I am working on a new project for developing the glucose biosensor based on electrochemical measurement. I don't have too much experience in electrochemical analysis and electrochemical biosensors, so I would like to ask some questions and I hope some experienced researcher can help me out.
1. I read several papers related to the glucose biosensor and in the papers, most of them were using the Prussian Blue as the mediator for transferring the electron from the enzyme (glucose oxidase) to the electrode. However, the synthesis of the Prussian Blue required dissolving the K3[Fe(CN)6] in the hydrogen chloride solution ( which is a strongly acidic solution). And I am worried about is there any risk of mixing the K3[Fe(CN)6] with HCl? For example, is there any possibility that there will be the release of HCN?
2. I want to use chronoamperometry for the measurement but I don't know how to select the appropriate potential for the measurement? I think I will do the measurement with the selected potential and based on the current change, I can determine the glucose concentration. So should I do the CV firstly and then which potential should I choose? Potential of peak current in the CV or others?
Thank you so much for the reading. And please give me some ideas. Thank you in advance.
Respected all, can u please suggest me blocking buffers for DNA based biosensors other than BSA and mercaptohexanol ?
I fabricate some gel bead as a kind of biosensor with DNA probe conjugated. However I found there was some kind of dust induced during fabrication. Can I use a stainless still mesh to filter (The reason not the plastic ones is that in my experience it has high affinity to DNA)? If the affinity is high then alternatively how can I isolate my bead with the dust (in centrifuge both appear in sediment)?
When designing an experimental setup and we want to buy a certain chemical, for example; let's say we want to buy penicillin; cas number: 61-33-6.
Then we realize that we don't have enough money for the experiment with this chemical. But related chemicals such as
113-98-4 (mono-potassium salt)
69-57-8 (mono-hydrochloride salt)
are cheaper and we decide to purchase one of these two products. But of course their cas numbers are different.
How logical and reliable would it be to continue the experiments in this way and try to make a publication?
Let's say I asked this situation to write an article in the field of biosensors.
I want to know that how biology can be related (in any way) for the study of MOSFET biosensors or FET based biosensors. Any suggestion will be highly appreciated.
I am working on biosensor with mvenous and mcherry. I fix the cells at different time points image them and then bleach it to remove the signals of sensor and then stain with Edu and DAPI. Now I have to use cell profiler to analyze them. Can someone guide me how to use the cell profiler to analyze it?
I've followed many articles to find out the relationship of various concentrations and the potential of GSH or GSSG in different cellular organelles but it's quite confusing for me to find out how do we convert these to different entities to each other?
Can someone please explain the Nernst equation to find out the potential of different cellular compartments?
I'm interested in research in fiberoptic biosensors, MEMS AFM microscopy, photonic biosensors.
If it is no problem could you suggest the cutting-edge area research fiberoptic biosensors, MEMS AFM microscopy, photonic biosensors for a research proposal?
Currently, I am trying to simulate a surface plasmon resonance (SPR) biosensor on a multilayer structure using FDTD Lumerical software. I am a beginner and have never used this software before. Are there any tutorials related to SPR simulation using this software? This document was very meaningful in completing my research.
In a typical paper around microalgae biosensor, an increase in current was illustrated with time by chronoamperometry. But, my observations showed a decrease in current with time and I wonder what problem may occur?
Could it be cause of electrochemical method or the kind of microalgae species?
Please share me your experiment and knowledge.
I am thinking of isolating the protein from soil or leaves sample with the pesticide.
But I am quite lost with the minimal medium I would use, Do anyone has a paper or an advice on the best way to go about this?
The antibodies to the protein of interest should be attached to the biosensor and thereafter this biosensor should evaluate the amounts of this protein in the organism's liquids.
I would like to fabricate a nanosensor for sensing application of biological molecules like cholestrol, uric acid etc
Please discuss how can I select a suitable polymer and the nanofiller (two dimensional material, MXene etc) to develop a new composite sensor material which would give good results using electrochemical workstation of three electrode system
Expecting valuable suggestions
I am trying to pack one bead per drop using microfluid. I previously assumed that just adjust bead concentration in accordance to how many drop per minute. Yet this route failed. I think I need to read more on packing cell or bead in microfluid, yet I didnot find many materials. Could you recommend any paper/protocol/note/piece?
I am studying on the biosensor about SARS-CoV-2 infection. I investigate the main Protease (Mpro). could you please inform me "what is the proteolytic product of "Mpro" at the end of the reaction? for example pH changes via proton (H+) releasing or other factor?
Thanks in advance
I realized many papers use scan rate 50mV/s or 100mV/s. But how do actually they determine the right scan rate to use throughout the experiment? What are the measurements that I should take into account in choosing it?
I am trying to make a DNA sensor but I am detecting non specific binding. Does anyone know a better blocking agent for DNA sensors based on rGO, apart from BSA or MCH?
I have troubles to read out the correct anodic peak currents from my cyclic voltammogram since ORR is pushing down my baseline. Is there anything (besides substracting a proper blank) that i can do (mathematically?!) to get correct peak currents? Nicholsons empiric equation only applies to reverse scans, as far as i understood, and doesn't help me...
Despite the fact of the tremendous number of research papers in the literature dealing with the usage of aptamers as molecular probes in biosensing, and despite the fact that this technology is almost 30 years old, there is only just few companies producing aptasensors and aptaessays and the majority of them are startups, and almost no commercial biosensor or kits is currently available in the market.
Can anyone explain to me why there is such gap?
I have a short stretch of 20 aa with strong binding affinity for a compound. I am trying to de some whole cell flourescence or visible biosensing technique based on this peptide. Can someone suggest a reliable genetic trick for the protein biosensors?
What media or salt solution (HBSS, EBSS, Tyrode) can be used for real-time intracelular h2o2 imaging? I am using a genetically-encoded biosensor based on roGFP and have to find the media that wouldn't have much buffering capacity as well as compounds acting like antioxidants, since it is important to see how the cells alone are coping with h2o2 adition.
Estimated duration of the experiment - 1-1.5 h.
Cells: ipsc-derived neurons. Standart Neurobasal+B27 media was designed to protect neurons from any kind of oxidative damage and contains loads of antioxidants, so h2o2 don't get the chance to get inside the cell.
Hi to all I immobilized a peroxidase on a new synthesized MOF. The reviewer of a journal paper asked me to consider the inhibitory activity and specific affinity of my immobilized enzyme in the introduction. How I can answer this comment of the reviewer for a journal paper revision? What does it mean? In my opinion, these items are related to biosensors. I reported the influence of immobilization on km in my article but I have no idea about the inhibitory activity.
In research publications, it is often said that electrochemical sensors and biosensors are simple, portable, and easy-to-use. However, optical sensors (mainly colorimetry and fluorescence) are widely used in real clinical analysis, not electrochemical (except glucose sensor). ELISA, PCR, or whatever the biosensors, colorimetry, or fluorescence read-out are preferable, while electrochemical biosensors are seldom preferred by clinicians, why?
I was wondering if there is any commercial LSPR biosensor based on gold nanoparticles aggregation for clinical usage, especially as a point of care testing (POCT).
I'm currently trying to develop a biosensor to detect covid19 infection. However, I don't want to be exposed to the real virus for a real practical test. Is there any way, to simulate viruses so that a researcher can use these simulators without worrying so much about being infected?
I was wondering how to calculate the copy number for a viral protein from the protein concentration? I.e. for 1 mg covid protein (N or S protein) what would be the copy number? Thanks in anticipation.
For a range of concentrations of protein, I am getting the I-V (Current-voltage) data from a Keithley sourcemeter. Accordingly, I am getting the resistance as the response of protein-protein binding on carbon nanotube on my biosensor. What formula should I use to get normalized response versus concentration gradient? Is there any good textbook on this to show the calculations step by step from scratch? Thanks in advance.
I am trying to design a boron nitride based biosensor that can sense the abnormality of microbial activities on fish skin. I am very curious that is it possible to have such an aptamer that can detect the behavior of microbes? Instead of knowing what they are, I care about what they do.
Can we do semi quantitative detection in lateral flow ?
Say for example if we have X concentration of antigen present in body and we want to detect above the X concentration of it in lateral flow , can we do that ??
I have come across a lateral flow device of SD biosensor in which they say if there is more than 20 ng/ml concentration of CRP in body it will produce colour.
Do anyone has idea how we can do that ?
My research is on biosensors. Now I have to use gold nanoparticles and PAMAM dendrimers to modify the electrode.
I am struggling to understand the interaction between the two maybe it's because of the structure of the dendrimers.
Can you kindly assist, clarify the chemistry between these two please.
I am looking for substantial evidence of effect of geomerty of flow channels (microfluidics) on the sensitivity and feasibility of Surface Plasmon Resonance (SPR) Biosensors with respect to different applications (whole cell, protein or DNA detection). I know one from Prof. Jiri Homola.
I need to design my own microfluidic channels for SPR sensing applications. Please guide me.
I want to know the most important Characterization for imprinted polymer molecular print in biosensor for formaldehyde detection.
There are a large number of sensor technologies available to detect a particular type of analytes.
I understand every platform has some limitation and some advantages among others.
In current situation which type of biosensors (FET/elctro-chemical/luminescent-based/electro-luminescence) provide more reliability to become a part of future point-of-care?
Could you please link me with an article related to the CNT based bio sensors?I am not expert enough due to my background to find out.
I have following materials :
1. Glutaraldehyde 25%
2. 3-amino propyl (terimethoxysilane )97 %
3. Glocuse oxidase
4.Any kind of agent acids
Please kindly help me to find out the concentrations and steps by linking me to a related article or any kind of reports,
I am working on research related to fabrication of glucose biosensor by using screen printed carbon electrodes (SPCE). I modified the working electrode with nanocomposite and then immobilised glucose oxidase-glutaraldehyde mixture on the modified SPCE.
Here are the detailed steps:
1. Preparation of nanocomposite.
2. Mix 20 μL of the 2% glutaraldehyde solution with 50 μL of Glucose oxidase. The mixture was allowed to sit for 30 minutes at room temperature before use.
3. Drop cast 3 μL of the nanocomposite onto the working electrode (WE) of SPCE.
Immobilization of Glucose oxidase-glutaraldehyde mixture:
1. Drop cast 3 μL of glucose oxidase-glutaraldehyde mixture on the modified SPCE
2. Allow the solution to adsorb onto the modified SPCE for 24 hours at room temperature.
3. After 24 hours, wash the unbound glucose oxidase from the working electrode using 0.01M PBS (pH 7).
4. Let the SPCE to dry at room temperature.
The MSDS mentioned that optimum storage temperature of glucose oxidase is -20°C.
As I am performing the immobilization of Glucose oxidase-glutaraldehyde mixture at room temperature for 24 hours, will it affect the stability of the glucose oxidase?
Plus, should I be storing the SPCE with immobilised glucose oxidase at room temperature or at cool temperature (probably 4°C)?
Help appreciated!! Thanks
I'm interested in very small changes of human skin temperature and so far I didn't found anything better than 0.1°C. I know there are a lot of sensors with a higher resolution but this unfortunatelly doesn't tell me anything about repeatability and precision. In a nutshell, I am searching for a sensor with high accuracy, high precision and high repeatability and would be glad to get some hints from the community.
I am trying to understand the use of interdigitated electrodes for use in biosensors and wondering: how to choose a configuration(geometric parameters) to start? Can I simply get a random one and try to optimize?
When testing a biosensor with different concentrations of antigen, it is said that the electrode should be flushed with PBS. Is PBS alone sufficient or the washing protocol with PBS+Tween 20 and PBS should be completed each time?
I'm preparing a biosensor from p- type silicon , i think etching is easier , it does not need illumination , but i need to know in scientific manner , it using p- type more affordable ? because i see that most of previous work the have chosen p- type also
I need nitrocellulose membrane for lateral flow assay for antigen detection. Having searched for other companies, they are only specialized for western blotting. So can the same nitrocellulose membrane be used for LFA also?
I'm currently studying the detection of uric acid using square wave analysis.
In several articles that I read, the uric acid (UA) was diluted using deionized water. However, from my experiment, it is impossoble to dilute the UA using DW only. I was also advised to use 0.2M NaOH, but the problem still cannot be solved.
Please assist. Thank you.