Science topic
Biosensors - Science topic
Device combination of biorecognition receptor and physicochemical transducer
Questions related to Biosensors
2025 5th International Conference on Bioinformatics and Intelligent Computing (BIC 2025) will be held on January 10-12, 2025 in Shenyang, China.
Conference Website: https://ais.cn/u/2qqmQv
---Call for papers---
The topics of interest for submission include, but are not limited to:
1. Bioinformatics
Algorithms, models, software, and tools in Bioinformatics
Any novel approaches to bioinformatics problems
Applications to diagnosis, prognosis, treatment, monitoring and rehabilitation
Biological devices/Biosensors and Molecular Diagnostics
Computational proteomics......
2. Bioinformatics & Intelligent Computing
Algorithms and Software Tools
Algorithms, models, software, and tools in Bioinformatics
Biostatistics and Stochastic Models
Computational evolutionary biology
Computational Intelligence
Computational Molecular Systems......
3. Intelligent Computing
Designing and building hardware & software systems processing,structuring
Managing various kinds of information
Scientific research
Making computer systems behave intelligently
Creating and using communications & entertainment media
---Publication---
All accepted full papers will be published in ACM ICPS (ACM International Conference Proceeding Series) and will be submitted to EI Compendex / Scopus for indexing.
---Important Dates---
Full Paper Submission Date: December 18, 2024
Notification Date: December 25, 2024
Final Paper Submission Date: December 30, 2024
Conference Dates: January 12-14, 2025
--- Paper Submission---
Please send the full paper(word+pdf) to Submission System:
I have designed and simulated a FinFET based biosensor for sensing biomolecules with high dielectric constant (k) i.e. k ranging from 2-10 with the help of Silvaco Atlas. The simulation of FinFET biosensor is converging and working fine when the cavity is filled with air i.e. when k=1. However, when the cavity is filled with high k biosensor molecules the simulation is not converging although we have adjusted the meshing and used electric field dependent mobility and transport models and other recombination and tunneling models for the simulation. We have used the Newton and Gummel methods to solve the models in the simulation. Can anyone give me his/her most valuable suggestions to resolve out the problem issue?
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer (thickness is around 300-900 nm). This polymer layer acts as a transducer, where it was developed to detect target components (bacteria in particular). This biosensor chip is used for (non-faradaic) capacitive detection of target bacteria. The EIS measurements are performed with PBS solution without using any redox probes (under 0 DC voltage), and two electrode concept is employed.
Regarding the AC amplitude, some reports in literature mentioned that higher (e.g., >50mV) AC amplitude is required due to the insulating polymer layer. Would you agree on this approach? How important do you think to optimise the AC amplitude to achieve the best sensing performance?
What are the key points to be considered while deciding the AC amplitude and How do you decide an optimal AC amplitude for non-faradaic electrochemical impedance spectroscopy?
Has anyone worked on nanomaterials for biosensors? What challenges did you face in terms of sensitivity and specificity?
Recently, I have been working on photonic sensors, and my latest project involves an innovative design for a cancer detection sensor using a photonic ring resonator structure in a label-free setup. In biosensors, three key parameters determine sensor quality: Sensitivity, Quality Factor (QF), and Figure of Merit (FOM). My unique optimization approach has resulted in approximate values as follows:
- Sensitivity (S): ~42-48 nm/RIU
- Quality Factor (QF): ~480,000
- Figure of Merit (FOM): ~13,000
Additionally, the structure achieves an impressive FWHM of 0.003 nm. For comparison, ideal ranges for these parameters typically are:
- Sensitivity: 50–300 nm/RIU (ideal: 200–500 nm/RIU)
- QF: 10,000–100,000 (ideal: >100,000)
- FWHM: 0.01–0.1 nm (ideal: <0.01 nm)
- FOM: 10–1000 RIU⁻¹ (ideal: >1000 RIU⁻¹ for high-precision sensors).
As the saying goes, “every achievement has its cost,” and there are always trade-offs in design. The high QF and FOM in my design highlight exceptional sensor performance, although the Sensitivity remains within an average range. Given these results, I am interested in feedback from the community—do you think these results are promising enough to submit for publication in journals focused on photonic sensors?
Please find attached the formulas for these parameters.
Your insights and suggestions would be greatly appreciated!
Hello everyone,
I’m exploring research in Virtual Reality (VR) combined with psychology, specifically to support Virtual Environment for Rehabilitation Therapy (VERT) for my master's thesis research. I wanted to understand the latest advancements, key research areas, or potential applications in this field. Could anyone provide insights or recommend recent studies or directions where VR is being effectively integrated with psychological therapy?
Thank you for your time and guidance
Best regards,
Stefanus Benhard
Electrochemistry, Biosensor
Can you imagine being able to control a virtual environment with your emotions? Or having a game adapt to your needs based on how nervous you get? More and more immersive Virtual Reality applications are incorporating biosensors, especially in the academic world. This combination can help improve human performance and well-being.
Immersive Virtual Reality (iVR) has revolutionized how we interact with digital environments due to its immersive capabilities, but when combined with a biofeedback system, this technology reaches a new level of interaction and personalization.
Biofeedback is a technique that acts as an immediate reflection of an individual's physiological response to stimuli.
HOW DOES BIOFEEDBACK WORK IN AN IVR APPLICATION?
- A VR application is created, and a biofeedback system is planned.
- Biosensors are selected to capture a physiological signal (such as HR or breathing).
- These data are analysed using algorithms (artificial intelligence) to interpret the individual's emotional state. For example, an increase in heart rate could indicate stress.
- The interpreted information is displayed in the virtual environment through visual or auditory systems that can be more obvious (such as directly showing the HR) or more subtle (modifying the environment or the difficulty of the experience).
- This biofeedback system helps the individual understand their physiological response and become aware of how to control it.
WHAT DO PEOPLE DO WITH THIS COMBINATION? We conducted a review of 560 studies to analyze how biosensors are used in combination with iVR, and here's what we discovered:
- There is no consensus on how to use this combination or how to design applications.
- This combination is predominantly used in Psychology and Medicine, although its use is also growing in areas such as Education or Risk Prevention.
- The most monitored signals are HR (53.3%), EDA, and EEG. Surprisingly, eye-tracking is not used as much despite being incorporated into some virtual reality devices.
- Mostly, Desktop 6DOF devices are used because, being connected to a computer, they have more power and greater capacity to connect in real-time with biosensors.
- Experiences are mostly passive (41.6%), using biosensors only to view physiological data and not for interaction. On the other hand, 40.5% of experiences are interactive. However, only in 17.3% of experiences is a biofeedback system used that utilizes biosensor data for interaction.
This is a very promising field because it combines several disciplines such as software development and medicine or education. We need to continue working to establish a common framework for the use of these biosensors and explore what else they can offer us. The possibilities are limitless!!
You can find these results and much more in our recent paper titled "A systematic review of wearable biosensor usage in immersive virtual reality experiences" in the journal Virtual Reality: https://doi.org/10.1007/s10055-024-00970-9
Hello everyone,
I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights.
Experimental Setup:
- Electrodes: Gold interdigitated electrodes (IDE) with a 1) 50 µm gap and width; 2) 200 µm gap and width.
- Solutions: DI water and 0.1X PBS buffer solution.
- Measurement Parameters: 100 mV amplitude, frequency range from 100 Hz to 10 MHz.
- Device: Microchamber with 350 µm height placed in a controlled humidity chamber. Cu tape with conductive epoxy is used to connect the device to the EIS spectrometer..
- Duration: Measurements taken at 2-minute intervals for 1 to 5 hour.
Observation:
- The impedance values show an increasing trend over time for 0.1X PBS buffer solution and decreasing trend for DI water irrespective of electrode size and gap.
- For comparison, a 1 kΩ resistor measured under the same conditions shows no change in impedance over time.
Question: Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit decreasing and increasing trend over time, while a 1 kΩ resistor does not show any change? What factors could be contributing to this behavior in my system? Is there anything related with 2 probe (working and counter electrode) system over 3 probes (working, counter and reference electrode)? If yes, then is there a way to attach a reference electrode to HP 4194A? We have EZstat-pro Potentiostat Galvonostat in our lab.
Thank you for your help!
What is the difference between reaction and interaction in chemistry? Would you please provide me with the details?
except BSA and in printed screen electrodes
Most of the research articles mentioned biosensor stabilty study conducted for 21 days Or a month. Is it a standard period of time to test the working stabilty of a biosensor?
Regarding AR2G sensors, If i need to do kinetic assay for binding between analyte molecule (such as nanoparticles have free NH2 and NH groups) and protein, (the nanoparticles are larger than the protein sample )
Are nanoparticles should be loaded over the biosesnsor (in assay running buffer) before exposed to the protein sample to assay the binding kietics?
- If yes, in this case the, theassay flow will flipped, I mean nanoparticles will be in loading step, while the protein sample will placed in the association step.
- If no and we have to follow Sartorius technical note, the protein should be loaded over the the biosensor (in acetate buffer) in loading ster, then exposed to nanoparticles in association step.
How I have to designe my experiment ?
Is BSA in running buffer will do non-speciefic bind ?
I need help from expert in Octet R8.
I have already worked in this field to detect the volatile organic compound in lung cancer using biosensor so now what further work can i do in the field of biosensor
Hello! Our team is developing a magnetoelastic biosensor for virus detection. We want to use a Fe-Ni magnetostrictive alloy (for example, Metglas 2826), but we can't find where to order it in low quantity for experimental tests. Metglas doesn't reply to our quote requests and another manufacturer (Vacuumschmelze) offers minimal order quantity of 450 KG.
Does anyone have experience of ordering this kind of materials in low quantities for lab tests?
Biosensors are developing day by day due to their applications. Each of these sensors is used in specific fields. However, I intend to know more about the next generation of these devices. Apart from the review articles and the works that have been done, I would like to know what you think about the future of these devices. For example, what features will they have? Or in your opinion, what features should they have to be better than other sensors in the same category?
Write me any ideas or comments you have about this. Thank you
I have used the nanoporous gold electrode to check cyclic voltammetry (CV) response in ferricyanide solution.
Now, I want to use the same electrode to check response of interaction with a protein after functionalizing the electrode surface with a thiolated receptor.
This second experiment will be conducted in PBS.
What could be a good protocol for cleaning the electrode before doing this experiment ?
So far I have only cleaned the electrode in PBS after doing the CV in ferricyanide.
Hy, I want to know proper protocol for biosensor preperation as well as methodology for chitosan biosensor preperation.
I am writing a book on biosensors for agrifood sector and I am looking for a researcher working on the use of drones for biosensor/sensor application in precision farming.
Currently I am working on SPR-PCF biosensor for biochemical detection , i am stuck in comsol multiphysics to find the sensitivity of SPR-PCF sensor how to do the analysis where to put the formula and how to check the results in comsol please someone suggest me the solution and please give the detailed explaination for that
I designed a aptasensor for tRF detection. I need to know what could be the usual practice for tRF concentration range to have a convincing application for plasma tRF detection? I need to understand it in ng/mL unit. I have the understanding that the tRF concentration would be in the range of 0.01-10 ng/mL for plasma, 50 ng/mL for urine, and 0.1-10 ng/mL for cerebrospinal fluid (CSF) samples.
I want to double check with someone who worked in this field and has some experience.
Molecular biology is, unfortunately, not my strong suit.
Thanks.
Biosensors, Electro-Chemical Sensors, Ionic conductivity
Hello
I need it urgently
Can you give me articles in the field of aflatoxin that are both biophysical and bioinformatics?(for example biosensor)
I'm developing a lectin biosensor. I have optimized all steps of the construction based on the impedance signal (delta Rct = Rct analyte – Rct blank) of one concentration of the analyte.
Now, I'm trying to do one calibration curve.
My biosensor does not distinguish different concentrations of my analyte. I tried to incubate the biosensor with the analyte for 30min and 5min, and I have the solutions more concentrated grouped and the solutions less concentrated grouped too.
What do you recommend?
Dear all
I have used UVO (UV-Ozone) treatment to create -OH end on SiO2 surface. It seems to work pretty well, but I can't find any articles explaining the exact mechanism. In my opinion, H2O vapor in the air might be the one supporting H atoms to form -OH ends. However, hydroxylation occurs anyway after quite a long gas purge with N2+O2 gas. My setup may not purge the air perfectly, but I'm still questioning the source of H atom during -OH end formation. If you know the mechanism of hydroxylation of SiO2 with UVO or have some articles about it, please let me know.
Thanks.
Lead magnesium niobate–lead titanate (Pb(Mg1/3Nb2/3)O3)0.65–
(PbTiO3)0.35 (PMN-PT) piezoelectric plate sensor (PEPS). We currently use high precision wire saw with PMN-PT piece mounted on the wax substrate to cut huge pieces into small size to make sensors out of it. The main problem is that, the yield of the cutting is very bad. After the sensor are cut, they are very small and fragile so handling is a big problem ( currently we use weighing paper to move the sensors). Is there an alternative way to handle small, fragile and thin PMN-PT?
Certain Elsevier journals like Biosensors and Bioelectronics: X and Analytica Chimica Acta: X are open access companions of their original counterparts. These companion journals do not have an impact factor but just a cite score.
Will these journals get an impact factor in the future?
I'm currently developing photoelectrochemical biosensors which require me to do EIS. The system I'm using is Autolab and I understand that EIS should be done under OCP for it to be more accurate. I tried finding online on the step to step procedures on this but to no avail.
I am looking for published data on the variation / strength / reach of the electric field around the working electrode for a typical 2/3 electrode electrochemical biosensor. Specifically , I am interested to model the electric field to understand the distance from the electrode where its effect / strength becomes negligible.
I want to understand how this limits the performance of biosensors, specifically those which measure impedance , capacitance etc.
i have uploaded image about question.i want to design some biosensor in electrochemistry. i need two chemical molecules (A & B). A is conjugated on a surface by some linker and B is connected to a electrode surface. when the linker breaks, A and B should joint each other especially with targeted linkage. A should have a property to induce electric current in B.
please guide me which molecules can be A and B?
I want to determine the glucose concentration of my samples using the Biosensor. However, my samples contain glucose oxidase (GOD) in order to remove hydrogen peroxide from my samples.
The principle of the biosensor is that the glucose in the sample generates hydrogen peroxide by glucose oxidase in the enzyme electrode. Then, the generated hydrogen peroxide is oxidized by the platinum anode. The glucose concentration is then determined by the change of the currect value according to this oxidation.
Because my samples contain glucose oxidase, the glucose concentration decreases.
I determined the glucose concentration of a known sample, having a concentration of 1.9 g/L. However, when GOD is added to my sample, the concentration decreases to 1.75 g/L .
Therefore, I want to remove glucose oxidase prior to using the Biosensor. How can I do this?
I am having some issues with Bovine serum I have purchased and am wondering if there is a better serum to purchase that others have had better success using? any advice would be appreciated.
I want to calculate the LOD for this sensor, based on the figures in this table.
I know that LOD is 3 x std LOB
But what is a method to decipher LOD from this data set?
Thanks
I'm using COMSOL Multiphysics SW for the design of biosensor.
I want to add peizoresistive material for the sensing purpose. But there is no peizoresistive Material in materials library. So, I hope I can add it from outside. But I don't know how add.
Hello everyone,
I had written two papers. One of the paper is on analog performance of GAA MOSFET and second one is on bio sensing performance of GAA MOSFET.
Both these papers are simulation based. I had sent them to various journals but unfortunately got rejected due to absence of any device physics( I am working on device physics in my current work-next paper).
I want to know if any Scopus or SCI based journal that can possibly accept these papers. I am really depressed since its been more than a year but its getting rejected. Any Scopus journal will also work but should be recognized.
Please, suggest me some journals seniors and respected people. Kindly help me.
DOMAIN- Electronics(VLSI) and MOSFET based Biosensors
In an ionic solution a charged surface also attracts counterions from the solution, forming an electrical double layer (EDL). This EDL effectively screens off the charge, and in physiologically relevant conditions, the characteristic charge screening length (Debye length) is less than a nanometer (nm). Thus, in high ionic strength solutions, charge based (DC) detection is fundamentally impeded. What are established solutions to overcome this problem?
A pH-responsive hydrogel was synthesized and dried. It swells at higher pH and shrinks at lower pH. How can this behavour be utilized in making a biosensing device?
Moreover, if superparamagnetic iron oxide nanoparticles are embedded in the pH-sensitive hydrogel, how can this material be used in making a biosensor?
Hi everyone,
I am working on a lateral flow rapid test using the sandwich method. I tried different parameters to get the result in the full test but no success. However, it works in the half test format.
When there is no sample and conjugate pad and I put the antigen and conjugate AuNPs in a well and run the half strip, it works and when I run the full strip it did not even show a faint line.
Any idea about what to do to solve the problem.
P.S. I want to use the strip for detecting the antigen in 100 ng/mL concentration.
The unique physicochemical properties of CNTs make them among prime candidates for numerous applications in biomedical fields including drug delivery, gene therapy, biosensors, and tissue engineering applications.
Limitations of carbon dot or carbon dot based products for commercialization.
We have designed a dual enzyme electrochemical biosensor that generates glucose at the end and ultimately to H2O2 to generate the signal with help of glucose oxidase. We would like to challenge the proposed biosensor in serum samples however, glucose that is already presented in those samples in high concentrations I suppose can interfere with the signal. Can someone suggest how to avoid the interference?
I am currently determining EIS spectra on different carbon-based electrodes. For this, I use a PalmsensPS4 at pretty standard conditions in a three electrode setup (0.1 Hz to 100 kHz) and measure against OCP in a 5mM [Fe(CN6)]3- / [Fe(CN6)]4- (1:1) in phosphate buffered saline (PBS) at pH 7. I then use EIS Spectrum analyser to fit the curves. One type of my electrodes results in data that can be easily fitted with a standard electrochemical Randles cell (yellow in figure). The other type, however, seems to have lower impedance overall, and very low charge-transfer resistance in particular, and I cannot seem to find a model that fits it (blue in figure). When I modify the electrodes with PEDOT, I get the same picture.
I have some questions relating to this:
(1) How can I explain, in EIS terms, what happened to these electrodes?
(2) Which model can I use to generate useful fits?
(3) Are there any papers in which these strange EIS curves have been obtained and are explained?
(4) Is it worth to change experimental conditions and what would you change to obtain meaningful data? I was planning to change the electrolyte concentration and / or the potential (though I am not sure if that would give any meaningful results).
Hello Sabine Kerstin Lengger
Don't mention it. We're happy to help. Like the medievals said, "respondeo dicendum":
(1) You can see your Bode plot (the -Phase one) with some peaks in this plot. I've attached here your plot with the max points I've told you earlier. So, in both experiments you have three maximum points in phase angle: in high frequencies (circa 105 Hz, which is the double layer phenomena in the electrode/surface region), another one in middle frequencies (circa 103 Hz, which is the charge transfer reaction), and another one in lower frequencies (between 100 Hz and 10-1 Hz, which is the electrochemical diffusional region). Since then, you have some interesting things here: now you can undersand better which goes on your electrode's surface, since there you have two behaviors: at high frequency the blue plot shows a very tiny semicapacitive circle (as seen in Nyquist's plot) but vanishes in the yellowed plot; the middle frequencies increased the phase angle (which means a decrease of resistive behavior of your electrode's surface), and finally in lower frequencies the diffusion changes between blue and yellow. So, maybe your modification improved the performance of ferri/ferro electrochemical reaction. Being a SPE electrode, the mass transfers changes a lot. For instance, the diffusion changes for a "semi-infinite" to a finite diffusion due to the diminshing surface area of your electrode. So, the classical Randles diffusional electrochemical circuit won't apply in your case and everything in your system looks fine.
(2) Yes, that's OK. The KK test isn't so easy to understand but their results are. See, your test showed a residual error less than 3% in all frequency range, and your KK data shows which I assumed in my answer: your system looks good.
(3) Yeah, impedance is beautiful but the current literature lacks on those experimental things. But I really like to discuss those electrochemical things in my research papers, and maybe sometime I'll publish some literature regarding this.
Hope it helps, and fell free to discuss anytime. 🤓
I am trying to get an accurate measurement of the size of a commercial carboxyl coated QD, vendor states the size is between 15-20nm. The sample is in a Borate Buffer at pH9.0, the same buffer the QDs were packaged. The scan has an intensity distribution: 70+/-111.1nm; volume distribution: 2.6+/-2.1nm; number distribution: 1.5+/-0.5nm. I am wondering why the volume and number distribution are reading low and not closer to the 15-20nm range as the vendor states.
Hi!
I am having some DNA probe conjugated on gel surface (polyacrylamide) and I want to perform reverse transcription and PCR after the DNA probe capture target RNA. Yet the volume of water absorbed in the gel cause a problem. How should I calculate and decide the amount of reaction mix to use? should I directly add same volume, 2X concentration onto the gel (20ul gel in a well)?
Thanks!
Hi!
It is commonly used of silica membrae membrane in DNA extraction column to bind and release DNA. Yet I wonder can I simply use extraction tube to filter and collect ~20um beads (conjugated with DNA), then take out the whole membrane for the downstream reverse transcription, exonuclease and PCR. Will the membrane block or impede the enzyme to access the DNA on spheres? The membrane will be fully immersed in the reaction mix.
Thanks!
organic field effect transistors OFETs for biosensors, chemical and optical sensors.
Is it necessary to do SAM before Fibronectin? With this modification, how long does it take for cells to spread on top of an electrode?
I have made a fluorescent protein biosensor that is sensitive to temperature. I will need to measure the signal at 30 degrees by flow cytometer. But normally, samples were run at room Tem. And the sheath fluid is also at room Tem. How can I measure the biosensor by flow cytometer at tem higher than room tem? Someone told me that the time from sample to the detectors is within mili seconds so it should be fine but I am not sure about that. Anyone has any suggestion?
Recently, I am working on a new project for developing the glucose biosensor based on electrochemical measurement. I don't have too much experience in electrochemical analysis and electrochemical biosensors, so I would like to ask some questions and I hope some experienced researcher can help me out.
1. I read several papers related to the glucose biosensor and in the papers, most of them were using the Prussian Blue as the mediator for transferring the electron from the enzyme (glucose oxidase) to the electrode. However, the synthesis of the Prussian Blue required dissolving the K3[Fe(CN)6] in the hydrogen chloride solution ( which is a strongly acidic solution). And I am worried about is there any risk of mixing the K3[Fe(CN)6] with HCl? For example, is there any possibility that there will be the release of HCN?
2. I want to use chronoamperometry for the measurement but I don't know how to select the appropriate potential for the measurement? I think I will do the measurement with the selected potential and based on the current change, I can determine the glucose concentration. So should I do the CV firstly and then which potential should I choose? Potential of peak current in the CV or others?
Thank you so much for the reading. And please give me some ideas. Thank you in advance.
Respected all, can u please suggest me blocking buffers for DNA based biosensors other than BSA and mercaptohexanol ?
QuestionAsked August 11, 2015can we calculate from slope of the linear concenterationI calculate sensitivity and limit of detection for biosensor?
Relation ship between sensitivity with electrode area and slope of the probe
I fabricate some gel bead as a kind of biosensor with DNA probe conjugated. However I found there was some kind of dust induced during fabrication. Can I use a stainless still mesh to filter (The reason not the plastic ones is that in my experience it has high affinity to DNA)? If the affinity is high then alternatively how can I isolate my bead with the dust (in centrifuge both appear in sediment)?
I want to know that how biology can be related (in any way) for the study of MOSFET biosensors or FET based biosensors. Any suggestion will be highly appreciated.
Many biosensors can be used to sense the infectious material, what is the extent of the usage of the optical biosensors specifically
Hi,
I am working on biosensor with mvenous and mcherry. I fix the cells at different time points image them and then bleach it to remove the signals of sensor and then stain with Edu and DAPI. Now I have to use cell profiler to analyze them. Can someone guide me how to use the cell profiler to analyze it?
Thank You
I've followed many articles to find out the relationship of various concentrations and the potential of GSH or GSSG in different cellular organelles but it's quite confusing for me to find out how do we convert these to different entities to each other?
Can someone please explain the Nernst equation to find out the potential of different cellular compartments?
Because I need to find out some nano biosensors to study.
it is under a module toxicology and biosensors
Hello,
I'm interested in research in fiberoptic biosensors, MEMS AFM microscopy, photonic biosensors.
If it is no problem could you suggest the cutting-edge area research fiberoptic biosensors, MEMS AFM microscopy, photonic biosensors for a research proposal?
Currently, I am trying to simulate a surface plasmon resonance (SPR) biosensor on a multilayer structure using FDTD Lumerical software. I am a beginner and have never used this software before. Are there any tutorials related to SPR simulation using this software? This document was very meaningful in completing my research.
Thank you
In a typical paper around microalgae biosensor, an increase in current was illustrated with time by chronoamperometry. But, my observations showed a decrease in current with time and I wonder what problem may occur?
Could it be cause of electrochemical method or the kind of microalgae species?
Please share me your experiment and knowledge.
can we calculate from slope of the linear concenteration
I am working on a project in which I need to do the molecular dynamics simulation of electrochemical DNA-based Biosensors using LAMMPS. Can anyone help me?
I am thinking of isolating the protein from soil or leaves sample with the pesticide.
But I am quite lost with the minimal medium I would use, Do anyone has a paper or an advice on the best way to go about this?
The antibodies to the protein of interest should be attached to the biosensor and thereafter this biosensor should evaluate the amounts of this protein in the organism's liquids.
I would like to fabricate a nanosensor for sensing application of biological molecules like cholestrol, uric acid etc
Please discuss how can I select a suitable polymer and the nanofiller (two dimensional material, MXene etc) to develop a new composite sensor material which would give good results using electrochemical workstation of three electrode system
Expecting valuable suggestions
Hi!
I am trying to pack one bead per drop using microfluid. I previously assumed that just adjust bead concentration in accordance to how many drop per minute. Yet this route failed. I think I need to read more on packing cell or bead in microfluid, yet I didnot find many materials. Could you recommend any paper/protocol/note/piece?
Thank you!
Dear friends
I am studying on the biosensor about SARS-CoV-2 infection. I investigate the main Protease (Mpro). could you please inform me "what is the proteolytic product of "Mpro" at the end of the reaction? for example pH changes via proton (H+) releasing or other factor?
Thanks in advance
I realized many papers use scan rate 50mV/s or 100mV/s. But how do actually they determine the right scan rate to use throughout the experiment? What are the measurements that I should take into account in choosing it?
Dear colleagues
I have troubles to read out the correct anodic peak currents from my cyclic voltammogram since ORR is pushing down my baseline. Is there anything (besides substracting a proper blank) that i can do (mathematically?!) to get correct peak currents? Nicholsons empiric equation only applies to reverse scans, as far as i understood, and doesn't help me...
Despite the fact of the tremendous number of research papers in the literature dealing with the usage of aptamers as molecular probes in biosensing, and despite the fact that this technology is almost 30 years old, there is only just few companies producing aptasensors and aptaessays and the majority of them are startups, and almost no commercial biosensor or kits is currently available in the market.
Can anyone explain to me why there is such gap?
I am trying to make a DNA sensor but I am detecting non specific binding. Does anyone know a better blocking agent for DNA sensors based on rGO, apart from BSA or MCH?
What media or salt solution (HBSS, EBSS, Tyrode) can be used for real-time intracelular h2o2 imaging? I am using a genetically-encoded biosensor based on roGFP and have to find the media that wouldn't have much buffering capacity as well as compounds acting like antioxidants, since it is important to see how the cells alone are coping with h2o2 adition.
Estimated duration of the experiment - 1-1.5 h.
Cells: ipsc-derived neurons. Standart Neurobasal+B27 media was designed to protect neurons from any kind of oxidative damage and contains loads of antioxidants, so h2o2 don't get the chance to get inside the cell.
Hi to all
I immobilized a peroxidase on a new synthesized MOF. The reviewer of a journal paper asked me to consider the inhibitory activity and specific affinity of my immobilized enzyme in the introduction. How I can answer this comment of the reviewer for a journal paper revision? What does it mean? In my opinion, these items are related to biosensors. I reported the influence of immobilization on km in my article but I have no idea about the inhibitory activity.
Electrochemical microrna biosensor
I was wondering if there is any commercial LSPR biosensor based on gold nanoparticles aggregation for clinical usage, especially as a point of care testing (POCT).
how to estimate the response time of the electrochemical impedance spectroscopy biosensor
I'm currently trying to develop a biosensor to detect covid19 infection. However, I don't want to be exposed to the real virus for a real practical test. Is there any way, to simulate viruses so that a researcher can use these simulators without worrying so much about being infected?
I was wondering how to calculate the copy number for a viral protein from the protein concentration? I.e. for 1 mg covid protein (N or S protein) what would be the copy number? Thanks in anticipation.
For a range of concentrations of protein, I am getting the I-V (Current-voltage) data from a Keithley sourcemeter. Accordingly, I am getting the resistance as the response of protein-protein binding on carbon nanotube on my biosensor. What formula should I use to get normalized response versus concentration gradient? Is there any good textbook on this to show the calculations step by step from scratch? Thanks in advance.
In research publications, it is often said that electrochemical sensors and biosensors are simple, portable, and easy-to-use. However, optical sensors (mainly colorimetry and fluorescence) are widely used in real clinical analysis, not electrochemical (except glucose sensor). ELISA, PCR, or whatever the biosensors, colorimetry, or fluorescence read-out are preferable, while electrochemical biosensors are seldom preferred by clinicians, why?
I am trying to design a boron nitride based biosensor that can sense the abnormality of microbial activities on fish skin. I am very curious that is it possible to have such an aptamer that can detect the behavior of microbes? Instead of knowing what they are, I care about what they do.
Can we do semi quantitative detection in lateral flow ?
Say for example if we have X concentration of antigen present in body and we want to detect above the X concentration of it in lateral flow , can we do that ??
I have come across a lateral flow device of SD biosensor in which they say if there is more than 20 ng/ml concentration of CRP in body it will produce colour.
Do anyone has idea how we can do that ?
Dear all
My research is on biosensors. Now I have to use gold nanoparticles and PAMAM dendrimers to modify the electrode.
I am struggling to understand the interaction between the two maybe it's because of the structure of the dendrimers.
Can you kindly assist, clarify the chemistry between these two please.
Hi,
I am looking for substantial evidence of effect of geomerty of flow channels (microfluidics) on the sensitivity and feasibility of Surface Plasmon Resonance (SPR) Biosensors with respect to different applications (whole cell, protein or DNA detection). I know one from Prof. Jiri Homola.
I need to design my own microfluidic channels for SPR sensing applications. Please guide me.
Thanks :)
I'm developing an impedimetric biosensor for lipase.
I want to know the most important Characterization for imprinted polymer molecular print in biosensor for formaldehyde detection.
Biosensors are successfully used for the quantitative estimation of several biologically important substances
Any one had developed product like Blood Glucometer for detection of COVID-19 infection
There are a large number of sensor technologies available to detect a particular type of analytes.
I understand every platform has some limitation and some advantages among others.
In current situation which type of biosensors (FET/elctro-chemical/luminescent-based/electro-luminescence) provide more reliability to become a part of future point-of-care?
Could you please link me with an article related to the CNT based bio sensors?I am not expert enough due to my background to find out.
I have following materials :
1. Glutaraldehyde 25%
2. 3-amino propyl (terimethoxysilane )97 %
3. Glocuse oxidase
4.Any kind of agent acids
Please kindly help me to find out the concentrations and steps by linking me to a related article or any kind of reports,
Personal Regards
I would like to immobilize boronic acid (3-APBA) solution on Grade 4 Chr Cellulose Chromatography paper. Which technique I should do?
Dear All,
I am working on research related to fabrication of glucose biosensor by using screen printed carbon electrodes (SPCE). I modified the working electrode with nanocomposite and then immobilised glucose oxidase-glutaraldehyde mixture on the modified SPCE.
Here are the detailed steps:
1. Preparation of nanocomposite.
2. Mix 20 μL of the 2% glutaraldehyde solution with 50 μL of Glucose oxidase. The mixture was allowed to sit for 30 minutes at room temperature before use.
3. Drop cast 3 μL of the nanocomposite onto the working electrode (WE) of SPCE.
Immobilization of Glucose oxidase-glutaraldehyde mixture:
1. Drop cast 3 μL of glucose oxidase-glutaraldehyde mixture on the modified SPCE
2. Allow the solution to adsorb onto the modified SPCE for 24 hours at room temperature.
3. After 24 hours, wash the unbound glucose oxidase from the working electrode using 0.01M PBS (pH 7).
4. Let the SPCE to dry at room temperature.
The MSDS mentioned that optimum storage temperature of glucose oxidase is -20°C.
As I am performing the immobilization of Glucose oxidase-glutaraldehyde mixture at room temperature for 24 hours, will it affect the stability of the glucose oxidase?
Plus, should I be storing the SPCE with immobilised glucose oxidase at room temperature or at cool temperature (probably 4°C)?
Help appreciated!! Thanks
I'm interested in very small changes of human skin temperature and so far I didn't found anything better than 0.1°C. I know there are a lot of sensors with a higher resolution but this unfortunatelly doesn't tell me anything about repeatability and precision. In a nutshell, I am searching for a sensor with high accuracy, high precision and high repeatability and would be glad to get some hints from the community.
I am trying to understand the use of interdigitated electrodes for use in biosensors and wondering: how to choose a configuration(geometric parameters) to start? Can I simply get a random one and try to optimize?
