Bioremediation - Science topic
Bioremediation is the use of microorganism metabolism to remove pollutants. Some examples of bioremediation technologies are phytoremediation, bioventing, bioleaching, landfarming, bioreactor, composting, bioaugmentation, rhizofiltration, and biostimulation.
Questions related to Bioremediation
I would like to get your opinions based on your experience on what other utilities can enzymes (Which can degrade petroleum hydrocarbons) be used for rather than cleaning the oil spills?
Culture media for Bioacclimitization of Cd and Cr by indigenous bacterial strains.
I was recently asked a question on whether biodegradation of microplastics into smaller plastic particles (e.g. nanoplastics) by microbial communities actually increases the toxicity of plastic particles and thus not a commendable bioremediation approach?
My immediate response was that the extent of biodegradation is of great importance when deciding on the effectiveness of bioremediation techniques. Given that the biodegradation process continues until mineralization of plastic polymers, then this approach could be confidently backed up. What are your insights on the matter? How could we defend bioremediation approaches knowing that degradation of microplastics might give rise to smaller plastic particles with behaviors that are harder to control and predict?
Any contribution is appreciated.
How bioremediation play an eco- friendly approach for environment pollution management? How are bacteria used for bioremediation and water environment?
I want to know the extraction of pesticide protocol from the soil sample in a suitable solvent so that it can be analyzed using GC-MS/LC-MS or HPLC for the presence of pesticide.
I recently joined as a Guest editor in Frontiers in Environmental Chemistry where I need to find team members as a co-editor to launch a research topic related to Biocatalysis, Bioremediation, Enzyme engineering, Microbial Enzymes, and Enzyme Immobilization. If anyone intrested to join mail me at email@example.com.
Will mail the detailed information to that person
I'm using Sporosarcina pasteurii to remove heavy metals from wastewater by producing metal carbonates. The issue I encounter is that high metal concentrations (i.e. Co 2g/L) strongly inhibit bacterial growth and activity.
One of the existing solutions is to isolate another already metal-tolerant strain (such as Lysinibacillus sphaericus). (source :
I have read that it is possible to adapt a bacterial culture to a high concentration of metals by serial acclimatisation, where the bacteria are successively grown in a medium of increasing metal concentration. (source : 10.1016/j.wasman.2018.07.010)
Can this method be adapted to ureolytic bacteria? Are there any examples?
If not, what other methods would you suggest?
Thank you !!
I have seen a great number of research papers reporting the use of pathogens such as Klebsiella pneumoniae for the production of biosurfactant for various use. But releasing such pathogenic strains directly in the environment may show pathogenicity in human and other animals. Hence, I am interested to know from the experts of this field that how do they justify the use of such pathogens for the production of biosurfactants?
I am looking for conditions and criteria for developing a microbial consortium for the purpose of bioremediation
Hi, friends.....I am working on white-rot fungi for bioremediation. the medium that I use is czapex. unfortunately, I don't see any growth while their enzymes are producing!!
These fungi have good growth on PDA and PDB medium but not in czapex. while all of the papers have reported with czapex
I am working on a Plastic Bioremediation Project and need to pre-treat the plastic waste samples before further work can be done.
Am currently undertaking bioremediation of hydrocarbon in soil using biostumulating agents (biochar). However, much has been talk about bacterial in this regards that is why i wish to check for fungi if possible...
I have heard that injections of yeast and molasses can slowly clean up a site contaminated with dry-cleaning solvents. Does anyone know of any scientific literature supporting this? Any information on this or any other methods that do not require landfilling of contaminated soil would be very helpful. We are more interested in soil than groundwater, but information on methods for either would help. Thanks.
In ex-situ bioremediation water aeration is essential for effective microbial activity. How can I estimate the amount of water to add to each amendment mixture during bioremediation process to obtain effective remediation for the chosen length of period?
Bioaccumulation is actually uptake of substances by organisms from their surrounding environment. It is a metabolically active and energy driven process. If this is to be considered as a type of bioremediation then it is to be accepted that bioaccumulation of pollutants by lower organisms and their subsequent transfer to higher trophic level (biomagnification) is also bioremediation ! And we humans are also partaking in this process !
So it would be more appropriate to consider biodegradation, biosorption, photo remediation etc. as executing processes of bioremediation.
I am engaged in remediation of industrial wastewater using biological methods like phytoremediation, but I am confused in choosing a suitable plant for phytoremediation of industrial wastewater. Please can anyone guide me for choosing the suitable plant for phytoremediation of industrial wastewater? In addition, what criteria should be used in selection of plant too?
Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend (insilico)?
I noticed that one of my novel species falls into the phylogenetic cluster of Pseudomonas aeruginosa and is closely related to P. alcaligenes (which was isolated from human blood samples and is also responsible for bioremediation purposes) based on genome tree.
Does this affiliation can be trusted with respect to the pathogenecity tendency?
Hello every one
My question is about how much roatation of centrifuge machine is required to process the sample taken out from flask incubated to study the bioremediation capacity of an bacterial strain.
and details of chemicals required for it.
I'm working on Bioremediation in a storm water drain which is receiving domestic sewage from nearby areas where proper sanitation facilities are not available. This drain is not a channelized structure means width and depth are not same along the complete length of 14 Km. It's receiving sewage through 5 lateral lines also. Total flow in the drain is 120 MLD (Million Litre per day).
I'm using Bioremediation process with strict and facultative anaerobic bacteria. My question is:
Can we calculate Mass balance in such drain where dimensions can't be calculated properly?
Secondly the concentrations of major pollutants are also changing tremendously with the flow?
If anyone having experience working such project please advice me for the same.
Bioremediation of plastic and polythene is a major environmental concern. In order to get rid of this utility of enzymes need to know. Please suggest recent development/literature for the same. Thank You..
Dr. Vinaya Tari
Bioremediation of diesel oil-contaminated soil-related question. possible ways of isolation would be greatly appreciated.
Bioremediation of environmental pollution/contamination/degradation in the less developed nations
Why microbial consortium is more effective in bioremediation of contaminants than the individual strains?
We need nigerian local substrates apart from spent mushroom for the bioremediation of crude oil polluted soil.
We want organic based substrates with high nutrient levels.
How do I interprete B and AT for metal adsorption? I found B and AT value 1.05 and 49.08 respectively. please tell the importance of the above mentioned parameters in biosorption?
I am working on a project where I am testing the resistance of a certain bacteria to copper and was testing it by adding copper at varying concentrations to r2a agar. However, despite sitting in a cold room for a week the agar at 0.08 M Cu 2+ is completely liquified and 0.00321 M Cu2+ is like a slushy. The other agar concentrations solidified. Is this happening because of the copper concentration and is there a way around this?
Many researches on Bioremediation are done in the Laboratory under a controlled environment, temperature, nutrient, and so on.. But.. How do we actualize bioremediation on the field where these factor can not be controlled, how do we ensure the survival on these microbes when introduced invivo?
My area of research is also bioremediation of pharmaceutical components in waste water samples. My query regarding to is there any cost effective method for analysis and detection of pharmaceutical into waste water .
my another query is on which basis i should select site for sampling
kindly assist me, I shall be highly obliged to you sir.
I am growing pesticide-remediating bacteria on minimal media (LeMasters Richards minimal media) where pesticide is the sole carbon source. The bacteria grows in a nice chunk so I know I have enough.
I've used the phenolchloroform with CTAB method, the Qiagen Powersoil kit, and sonication using Colvaris. I've been in contact with the companies to adjust the protocols to see if I get any results, nothing. I do multiple PBS washes of the bacteria every time. I tried heating the bacteria up and incubating the bacteria in lysozyme. Nothing seems to work and I don't understand why.
I ended up getting a little bit of that bacteria and growing it in LB which is isolated just fine; however, I am interested in eventually getting transcriptome information so I need the isolation to be in the minimal media.
Does anyone have an idea of why I get no DNA from this bacteria growing with pesticide, or other possible tests I can perform?
I am trying to search some literature on genes involved in degradation of classical naphthenic acids (NA). I have previously worked with CYP and ALK gene family but they are mostly studied for n-alkanes degradation, i.e. hydroxylases. Here, my question is that what is the scope of these genes being involved in the degradation of NAs. The substrate range for these genes is mostly up to C22, and they generally target straigh chain compounds; however, NA are often higher than C18 and are cyclic carboxylic acids. Can someone comment on it based on her/his experience? I would love to hear speculative answers/suggestions too. And if some knows that which genes are specifically involved in NA degradaton, or similar model compounds, I would appreciate to know that as well.
Thank you very much!
By applying a patented technology we can induce aerobic condition in the groundwater of a remediation site. Simultaneously we can influence the redox potential. I am struggling to find a reference in the literature that point toward an optimal potential for the metabolism of these two contaminants to occur in AEROBIC condition.
Several studies mention using agar amended with mercuric chloride when isolating mercury resistant bacteria. Is there a lab method available for preparation of these plates? If no, what are some details to consider when working with mercuric chloride agar?
Why are trace elements or vitamins not included in some of the bacterial minimal media like Bushnell Hans media?
Are trace elements not essential for bacterial growth?
If, for example, any carbon source like Tryptophan is used in such a minimal media without any trace element, will the Tryptophan degrading bacteria will grow in it?
In immobilized algae beads, nutrients such as NH4 and PO4 get adsorbed by the immobilizing matrix (such as alginate), following which they are assimilated by the microalgae via process of photosynthesis. In most research studies, the removal of nutrients by immobilized algae beads is much higher than blank beads. So is the assimilation process faster than the adsorption process?
The composition of the BH medium is as follows:
MgSO4 - 0.2g/L
CaCl2 - 0.02g/L
KH2PO4 - 1g/L
K2HPO4 - 1g/L
(NH4)2SO4 - 1g/L
and FeCl3 - 0.05g/L
I am currently working on hydrocarbon degradation studies using microorganisms. I'm worrying that the hydrocarbons might attack the nylon membrane filter. Thanks for the response!
I am presently working on a bioremediation experiment on PAH contaminated sediments with ammendment of various organic nutrients and results show high increase in PAHs (both low and high molecular weight) at the end of the treatment . There is certainly an interference with other organic metabolites because for each of the various bioremediation treatments, the higher the bacteria abundance, the higher the increase in PAH at the end.
I am presently trying to rinse the sediments twice with deionised water before analysis to solve the problem of interference. What else could I do?
I am pursuing my PhD degree from vit university, india. I wish to apply for short term visiting fellowship (3-9 months) in different countries (except USA,UK, FRANCE, KOREA). My domain of research includes the study of microbial diversity in high background radiation area and their adaptive strategy. I passionately want to work in the field of radio ecology and bioremediation of radioactive waste which are similar to my research interest.
I look forward to any suggestion of research laboratory/ institutes/ universities related to my field of research.
Thank you all in advance.
Hello everybody I have a question for my small research. Which is a better parameter to describe the effect of heavy metal toxicity in plants- is it biomass (wet weight) or dry weight?or both are appropriate ? And if both of the parameters are appropriate what is the relation between the parameters and heavy metal toxicity in plants ?
Thank you, and sorry if I am asking too much
Currently, we are in a process of editing a forthcoming publication entitled "Pesticide contamination of freshwater and soil environs: impacts, threats and sustainable remediation approaches", to be published by Apple Academic Press (AAP), Exclusive worldwide distribution by CRC Press, a Taylor & Francis Group.
Following are some tentative titles
Environment and Pesticide pollution
Pesticide pollution vis a vis Human Health
Chemical Pesticides: integrated methods in assessment and monitoring
Pesticide pollution: risk assessment and vulnerability
Bioavailability and bioindicators of pesticides
Pesticide contamination in water: Perspectives and Concerns
Pesticide pollution in soil: Exposure and hazards
Pesticide bioaccumulation: A threat to ecosystem services
Bio-pesticides: Importance and Challenges
Bio-magnification: Process and associated threats
Pesticide contamination and Agriculture
Bio-pesticides and Organic Agriculture
Integrated Pest Management vis-a-vis Bio-pesticides
Biocontrol Agents in organic agriculture
Pesticide pollution: Management and Challenges
Pesticide Remediation: methods and importance
Pesticide bioremediation: issues and challenges
Microbiological aspects of pesticide remediation
Advances in pesticide bioremediation technology
Role of biotechnology in pesticide remediation
Phytoremediation of pesticide-polluted water and soils
Microbial degradation of complex pesticides
Nanoremediation: Lab to Land approach
Wood chips and bioreactors for sustainable treatment of pesticide contaminated water and Soils.
Interesting contributors can text their e mail ids so that we shall send them the official invitation.
2nd Springer Euro-Mediterranean Conference for Environmental Integration (EMCEI 2019: www.emcei.net), 10-13 October 2019 in Sousse, Tunisia
1. EMCEI-2019 has now opened to receive submissions until 15 May 2019. 2. Accepted papers will be published in the proceedings by Springer before the conference. 3. EMCEI-2017 proceedings by Springer was indexed in Web of Science (ISI). 4. Best extended papers of EMCEI-2019 will be published in Springer journals after the conference. 5. More details at: https://www.emcei.net/
I am working on isolation of heavy metals resistant bacteria that can be used as microbial inoculant in bioremediation of metals polluted sites and have been using LB agar as my isolation medium. But I read that LB medium is not a good medium to be used in isolation so I need clarification on this.
I am about to do PAH analysis on crude oil containing my fungal samples. I have tried methods such as using hexane to separate my fungal samples from the oil but to no avail because the organic matter refused to separate from the oil.
I am faced with the situation of possibly doing PAH analysis without separating the fungi sample from the oil. I am not sure if the presence of my fungal samples will have any effect on the results of the PAH analysis or if the PAH analysis would work. The PAH analysis method I would be doing is the IP 391.
Hello! Im working on my thesis on Fungal Bioremediation and I noticed that my subcultured fungi took on a different color compared to our initial inoculation of it on PDA. I would like to ask if it is possible that fungi morphology could change if the fungi is exposed to different environments or media?
Hi i am Dr. Zakuan from International Islamic University in Malaysia. I am a senior lecturer in the department of biotechnology. My research focus is on environmental remediation (bioremediation and phytoremediation). In our department, we do have a lot of underutilized research laboratories due to lack of basic research facilities for specific research. Current situations are not allowing us to purchase any equipments anymore. Even research grants are so limited and very small to be used to purchase basic lab facilities. Therefore, i am looking for potential international collaborators who would like to invest of facilities in our laboratory.
I have downloaded hundreds of materials online but none of them gives me the detailed methodology I see.
Most of the works do emphasize on sand/soil whereas I seek just for river body/ water column.
Essence of the methodology is to know the method to use and also to find out what (and why) the concentration of the white rot fungi to contaminated water used for the mycoremediation.
Please I red experts on this and also has materials.
A partner from the European side of the Mediterranean area is urgently needed for an international project in the following topics:
Explore and exploit new metabolites and biomolecules, enzymes and genes; promote biotechnological applications and patent deposits
Evaluate the potential of marine litter from macro and micro-pollutants, including nanomaterials and plastics; assess its impact on marine organisms and develop in situ bioremediation actions
Am working on bioremediation using some particular plants, I want to determine if there is a gene resoponsible for the effective remediation by the plants.
I am working on an experiment in which I used industrial effluent to grow water hyacinth.
I was just wondering if I could use an effluent stirring system (continuously) to facilitate maximum exposure of pollutants to the roots of plant?
What is the possibility of this process being it be helpful and feasible?
i am asking about the extraction of heavy metals from plant tissue which use in bioremediation of polluted soils by HM ?
also we are preparing fungal culture to assess its ability in HM removal in labs.
Thank you for Help
I am working on myco-remediation of heavy metals by using mushrooms and I want to demonstrate the metal removal during the growth phase of mushrooms, because certain mushroom species actively captures metal ions from their contaminated substrate.
My question is that can I use BET theory to validate and present my experimental data?
Then please could anyone tell me what are the steps to follow?
Then, why? and Which possible and exact model can work in my case?
The mechanism of removal is given in the attached image.
Thank you !
We have used microbes n the recent past with some positive results, We also need a salt water resistance one for use with our other applications at sea.
I have been doing my literature review on bioremediation but considering my non biological background, I am unable to decide which option should I be further going deeper: bacterial remediation or phytoremediation
Micro-bioaugmentation technique is used for heavy metal removal from soil. i need details about this process.
hi, everybody, I wondered if there is a fastest and sure method or protocol for determining the electroactivity of bacteria and yeast in few day other than potentiostat/galvanostat .
aerobic and anaerobic treatment during secondary treatment level is a part of bioremediation. I am looking for another methods of bioremediation of wastewater treatment??
I'm planning my Undergraduate Research Project and am very interested in the field of Bryology (I'm majoring in Botany), and Plant Soil Feedback, but am having a tough time coming up with a topic. I thought I wanted to do my project on the effectiveness of bryophytes in bioremediation of hydrocarbon contaminated soils, but my interests have since shifted. Thanks in advance!
I was more concerned about the industrial pollutants been contained in the sludge. While dealing with wastewater coming from whole city including both domestic as well as industrial wastewater is having huge concentrations of varied nature of chemicals. Should bioremediation be considered as a viable option or there is some better way out to deal with this scenario?
Dear all, we are currently working on Bioremediation of heavy metal by using fungi with the help of formula;
Qe= (Ci-Cf)V/1000 M.
Whereas Qe = mg of metal ions uptake per gram biomass (mg/g), Ci=initial concentration of the metallic ions (mg/L); Cf=final concentration of metallic ions (mg/L); M=dried mass of the biosorbent in the reaction mixture (g) and V=volume of reaction mixture (L).
While, We have following supposed values;
Ci= 381 mg/L
V= 50 mL
Dry Cell Weight (M)= 7g
Anyone having expertise in this formula Please help us. As we have tried but final result should be in mg/g which is quit contusive. Thanks
what are the current research topics in the field of bioremediation of contaminated soils ?
why is MG1655 used to conjugal transfer using as donor strain WM3064 ?
this mechanism is used in article= Toward Bioremediation of Methylmercury Using Silica Encapsulated Escherichia coli Harboring the mer Operon
What are your views on the use of OUR (oxygen uptake rate), SOUR (specific oxygen uptake rate), BOD (Biological Oxygen Demand) and DO Dissolved Oxygen) in measuring biodegradation and bioremediation of crude oil?