Questions related to Biophotonics
1. The necessity of a polarization controller for single-mode fiber. Is a polarization controller necessary for single-mode fibers? What happens when you don't have a polarization controller?
2. Optical path matching problem. How to ensure that the two arms of the optical path difference match, any tips in the adjustment process? If the optical path difference exceeds the imaging distance, will interference fringes fail to appear?
Only these questions for the time being, if there are more welcome to point out.
There are some interesting challenges for DAS systems in fields of agri-biophotonics and/or biophotonics - from vibration impact studies on the roots of growing plants to sea fauna acoustics monitoring, but researchers usually prefer array of single sensors or quasi-distributed sensors. Or maybe you know the examples with the DAS application? Thank you!
I have recorded hologram is best on digital in-line holography technique. The distance between the objects and the sensor (CMOS sensor,taking from web cam) is typically 5 mm. The illumination source is LED ,that is placed at a distance of 5 cm from the sample and is filtered through a large pinhole having a diameter of 0.1 mm .
I'm searching a valid tutorial book to understand the principle of machine learning theory in order to apply directly to solve some problem in the field of bio-photonics to classify some species. I use Matlab and so I will prefer to use it as solver.
If I am interested in lasers, optical communication, nanomaterials, electro-optics, and biophotonics, what organizations should I join as a student?
Edit: I am seeking an organization that would help me in my research.
there is a lot of data, opinions and the like available about Biophotons. Many papers reveal, that organic matter may emit photons and it is argued that cells communicate by this.
If this is so, than there should be evidence that cells also ABSORB biophotons which other cells emitted.
How has this been proved?
what does optical output scaled by photon number mean? what is the difference from normal optical output calculations, for example if we are calculating optical efficiency or relative optical efficiency for an optical system(CPV), what does scaling by photon number mean and how does the results change from the regular calculations ?
I do not have access in the ICRU report 46 and I am not able to retrieve complete information regarding the tissue composition of prostate, urethra and rectum for a Monte Carlo simulation of brachytherapy that I want to do. Is there any knowledge regarding the composing elements, their ratios and the density of each of these three structures? Any bibliographic reference is also well appreciated.
Thank you in advance,
I am working on 'Quantification glistenings in IOL's using Scheimpflug images &ImageJ software', for the further analysis I need to use a macro(in Excel) to calculate the light scattering against the light scattering in the aqueous humor. Anybody can help me with the macro or any advise for analyzing?
After time lapse imaging of live cells, I need to track single cells to analyze motility from a series of bright field images in stack. Looking for a software which does that. I found a plug in called Trackmate in Fiji (an extended ImgaeJ version) but wonder whether that plug in can track cells in images other than those obtained from fluorescence imaging.
Hello guys as we know Bessel Beam Microscopy (BBM) is a near to far field microscopy, Can anyone explain its limitations and possible solutions???
Hello dear researchers, I have a question as we all know that the evanescent field is a non-propagating field it die quickly with distance which means photon counting decrease , I want to ask that is polarization status still there in those photons in other words is polarization continue keeping its persistency even after 2 to 3 microns even when the field is much low. if that then it can give us the pattern of the near field? Am i right? Please give your comments? if we go with polarization status then can we get more information about near field signals and to a larger range?
I am currently working on non-invasive blood glucose measurement using photoacoustic spectroscopy in near IR region(905nm). While using the laser source, what optical power is advisable? Is there a limit on usage of laser on skin?
It is obvious that biophotonic emission rate depends on various factors like age, growth factor, etc etc and the question may seem a little vague.
But I would like to know
#1: what would be the average biophotonic emission rate of a single human cell? I am mentioning it to be average, so as to temporarily compromise on the various sizes of cells in human body
#2: Available literatures broadly classify the spectral range to be 300 - 650 nm. Please let me know if data on spectrum is available on a single cell level.
Hi , I want to ask that if one want to study the ocean animals under the polarization microscope, then what are the animals whose skin has more anisotropic structure and one can study them( I mean their cell imaging, the scattering from their cells, imaging their cell etc.) better under polarization microscope???
Are there any recent investigations carried out in connection with biophoton emissions from a dying person? or, at least, do we have investigations on various bio-emissions (which includes EM spectral emissions over various bandwidths) associated with a dying condition? Also, any recent Gas Discharge Visualization (GDV) studies on a dying person?
Suppose, if we are able to detect certain bio-emissions (having the specific bandwidth) other than in the Infra-red (IR) region (which is usually connected to the metabolic activity), then we can associate these emission frequencies to the fundamental oscillations or modes at which communication (both inter- and intra-) happens. These modes can then act as the interface between mind (non-material aspect), body (material aspect) and the environment.
If we want to understand how a material brain could have probably constructed a non-material mind, then we need to aim at studies of field emissions from various parts of the body at the dying condition, this in a way helps in understanding location of the self or consciousness in the body (which may or may not be fundamental to the brain). This will give a glimpse as to how one's consciousness is connected to biofield/bioelectromagnetic phenomena and its probable location.
Is there any recommended standard samples for performing tracking measurements (2D)? Before going to any complicated measurements, we want to run some known samples.
I am wondering what is the importance and how to interpret these experiments. Also, i noticed that i can't have background corrected (No blank subtraction) for these experiments using perkin elmer equipment. How to solve this problem?!!!! Besides, I appreciate if you could send me some resources to read about 3D experiments.
Thanks in advance,
I performed a FCS experiment on DMPC bilayer tagged with BODIPY C12 HPC.
When I increase the power of excitation laser, I observe reduction in transit times.(see Fig 1) It is effectively like I am attaining smaller and smaller PSFs with increase in excitation laser power. We are using a LEICA TCS SP5 II microscope where power can be increased/decreased by adjusting AOTF.
I am not able to understand the reason behind this trend. Is it any form of artifact? Please give your comments.
I would like to make clot of EDTA added blood sample using CaCl2. I have 1 ml of blood with EDTA. What proportion of CaCl2 solution I should add to make a clot? I have added one drop of (2.5 ml water + 0.5M CaCl2) solution in 1 ml of blood but clot was not formed. Please let me know any possible procedure related to CaCl2 in order to form clot.
Hello, as a newbie in THz measurement, I encountered something strange when I tried to remove the absorption peaks in the THz measurement by pumping nitrogen into the chamber.
I found that most of the absorption peaks can be removed, such as the ones around 0.55, 0.75, 1 THz, 1.22 THz and 1.42 THz; however, the ones around 1.11 and 1.3 THz could not be removed clean even though other peaks were already unnoticeable and they just stayed there no matter how long the nitrogen pumping was. Does anyone have similar experience and how would you solve the problem? I was surprised since if these peaks are all from water vapor in the air, why they don't disappear simultaneously?
It seems that light propagation in tissue like skin can be modeled using ray tracing softwares, though in most of references Monte Carlo method has been used for this purpose.
I need to obtain spatial intensity of light (as well as angular intensity) over the skin when it is lightened using a light source.
So need to know how it is possible?
If possible what softwares are available?
Thank you very much in advance.
When the frequency of light is equal to the natural frequency of a molecule, molecule absorbs light.
My question is whether the molecule can have only one natural frequency? If thats the case why the natural frequency should match the energy gap between the ground and excited state.
If so, how? Do I need to divide the volume into two parts instead?
For instance, if I need to use a sphere in a sequential mode, I should use two hemispheres in contact. For an ellipsoid, rotationally symmetric for easiness, should we also divide it in two parts in contact, with one surface in respect to the anterior vertex and the second one in respect to the posterior vertex?
Any advice is welcome.
I am modelling the oblique incident reflectance profiles using a diffusion model, but would like to examine the range in which it is valid.
What is the reason that requires waveguide propagating the light used in OCT imaging to be single mode? What are the disadvantages to use multimode waveguide for OCT?
I am interested in the reasonable alternative to Qdot® 655 from Invitrogen Life Science Technologies.
Applications: Two Photon Intravital Microscopy of blood vessels (Organic dyes are not optimal).
I would appreciate any suggestions: quantum dots supplier or commercially available quantum dots for in vivo applications.
Has anyone defined a theoretical lower limit for the concentration of bonds that CARS is able to image?