Science topic
Biomaterials - Science topic
A biomaterial is any matter, surface, or construct that interacts with biological systems.
Questions related to Biomaterials
I need to apply for presentation in international conference in asea on March-April 2025, and i focus on China, firstly. Due to many websites on the internet are fake conference, and i am afraid of being deceived. So, please suggest me a reliable website for find the international conference. I am interested in apply chemistry or biomaterial or food packaging or polymer, or related those topics.
Thank you for your suggestion.
I am looking for possible reason why some organic acids are successful for collagen electrospinning and others are not exploited at all, as food grade solvent is a concern. Some explanation for possible criteria of selection of solvent.
After PVA and Boric acid are cross-linked, some gel formation occureated, but a cloudy residual liquid remain. How can we utilize this remaining liquid to get maximum efficiency?
Dear Researchers, Industry Professionals, Scientists, and Academicians,
We are editing a Book with ASME. This book delving into the specific applications of nanotechnology in biomaterials manufacturing is required to capitalize on the revolutionary promise of this technology while navigating the accompanying obstacles It can be a significant resource for engineers, researchers, and industry experts working on the cutting edge of materials science and biomedical engineering It is essential to address the challenge of creating environmentally friendly methods for synthesizing nanoparticles with specific characteristics. Nanoparticles encounter issues such as aggregation, contamination, and low yields, which affect their economic viability Accurate characterization and monitoring of nanomaterials during manufacturing at the nanoscale are crucial for advancement High resolution imaging techniques like scanning electron and atomic force microscopes are instrumental in this process On the other side concerns persist regarding the potential health and environmental risks associated with engineered nanoparticles in manufacturing Extensive research is necessary to comprehend their impact on human health and the environment The integration of nanotechnology into current manufacturing processes presents challenges such as high costs, scalability issues, and the complexity of converting nanomaterials into practical products The book offers valuable insights into how biomaterials can facilitate a smoother integration of nanotechnology into future manufacturing processes Unraveling the Potential of Nanotechnology in Next-Gen Manufacturing Processes A Perspective of Biomaterials" offers a thorough analysis of how nanotechnology is expected to transform the
manufacturing industry, especially with the creation of sophisticated biomaterials that improve productivity, sustainability, and product quality.

I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations for the second time. Like the highest quantity of a biomaterial is having lower concentration of protein than that of the lower quantity. For example, 2mg of biomaterial have 302.3 ug/ml conc. while 2.5mg has 344.2ug/ml conc. of protein and so on.
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations. Tried twice but similar inconsistency. Like the highest quantity of a biomaterial is having lower concentration of protein than that of the lower quantity. For example, 2mg of biomaterial have 302.3 ug/ml conc. while 2.5mg has 344.2ug/ml conc. of protein and so on.
I'm currently working with gelatin sponges which have a tendency to float, however, ideally I would like them to remain at the bottom of the well plate as intend to vulture cells on them. Is there an adhesive that I can use in this instance? I would also like to be able to take the sponges out relatively intact for analysis so the adhesive shouldn't be too strong I think.
What are the mechanical properties of the maxillofacial material Tera Harz TC-80DP? What works contain such information? Thank you!
What kind of material is Tera Harz TC-80DP for maxillofacial surgery? What is its composition? Where can I view SEM images of this material? Thank you!
Biomaterial Question
Hi. I have been trying to synthesising gelatin microparticles using double emulsion method. After adding the oil-gelatin mixture in the ethanol with stirring, the particle seemed to formed but when I washed it with acetone and centrifuge, the precipitates seemed to formed big ball and when I tear it apart it seems like gelatin fiber (instead of microparticle).. Just curious does high speed centrifugation affects microparticle formation? Thanks 🙏
I checked the literature in detail to compare different cell viability, cytotoxicity studies to check the biocompatibility of biomaterials. In some articles, they use the 'leachable-conditioned medium' method to check the biocompatibility. For this purpose first, they incubate biomaterials in cell culture medium without FBS after that they use this 'conditioned medium' with FBS in cell seeding. I am wondering in this first step why they don't add FBS directly to cell culture media and is it important for this procedure. I also saw different methods including FBS.
I am waiting for your response. Thanks:))
Please advise the works (articles, dissertations) that describe the principles and methods of controlling the structure of polymer-ceramic bone substitutes, the requirements for the structure of such products, the principles for choosing such substitutes for use, the pros and cons of using these products. Thank you!
What 3D polymers with carbon, oxygen and phosphorus, but without hydrogen, are used in maxillofacial surgery?
I have observed opposite response from gram negative and gram positive bacterial cells on plasma modified polymeric surface in terms of adhesion and biofilm formation.
gram negative cells show above 90% reduction in adhesion and gram positive show only 9% reduction. What could be the reason behind this?
Which property of bacterial cell type might be playing a role?
Please explain.
Thanks in advance.
Dear all,
I am interested in using NQR as a method for detecting the presence of people or animals in certain areas. My questions are as follows,
1)Whether there are suitable molecules or nuclei (some candidate nuclei including N and Cl) in these biological materials with NQR characteristics when a suitable radiofrequency field is applied, NQR characteristic spectra can be generated.
2)If such a compound exists, and whether it can be detected. Because some are products of the process, or not in solid form.
Thanks.
I am referring to the method of Kokubo and Takadama (2006) to prepare SBF solution, but I am not sure what is the ion-exchanged used in the method.
Nanoindentation is a attachment with AFM or it is a separate testing procedure? Nanoindentation gives property at nanolevel? Young's modulus, Hardness, Stiffness, Load vs Depth, Load Vs Hardness properties alone cane be obtained using nanoindentation or any other properties can also be known using nanoindentation? Where I can get all these things done in India? Please share your suggestions. Many of the prestigious institutions saying machine under maintenance, machine not working or operator not available.
Which works contain information about the mechanical as well as biological properties of the cerabone AW biomaterial, namely biodegradation. bioactivity, biocompatibility, osteoconduction, osteoinduction? Thank you!
Please tell me a scientific source, where there is information about some kind of biomaterial based on hydroxyapatite, namely: compressive hardness, Vickers hardness, information about biodegradation, biocompatibility, bioactivity, osteoinduction.
Thank you!
Tell me the sources where there is information about all mechanical parameters, as well as about biodegradation, biocompatibility, bioactivity, osteoinduction, osteoconduction of any biomaterial based on hydroxyapatite. Thank you!
I have quaternized the polymer to confer/enhance the antibacterial properties of the biomaterial. I need help whether increasing the amount or degree of quaternization of the polymer has better effect of antibacterial properties. I have achieved 6.5% of degree of substitution. Is this enough or do I need to increase this?
Over here oven drying method is commonly used and it requires you dry for a certain number of hours to determine the moisture content.
Is it that I will commence the moisture content determination at various times interval after oven drying?
Dear all
Hope you are doing well!
What are the best books in Materials Science and Engineering (Basics and Advanced)? Moreover, what are the best skills (or materials topic related) that materials scientists have to develop and to acquire?
Thanks in advance
^_^
My current focus on research is HAp production from biologically waste materials. I need to dissolve HAp for further characterization studies, however i got problems to dissolve it. so pls tell me any method or reference or solvent to dissolve HAp?
Hello everybody! :)
I apologize for the non-technical questions, but I hope you could help me! I feel like after the covid era I am still a bit "slowed down". Today I realized, that I forgot to check whether there are any conferences in tissue engineering, biomaterials, nanofibrous etc. during this summer 2022. I would truly like to FINALLY go somewhere, talk to other scientists in the field, present my research or at least to have a poster. Unfortunately, I wasn't able to find any conferences this summer, which are still open to applications. :( Could you, please, help me with this? Maybe you would have an idea about some local conference or summer school at your university (or research centre) in this field.
Thanks for your help and hopefully let's see each other during some summer appointment. :)
Marketa
I want to use a biological tissue for the SEM but I don't know how can I prepare the sample before drying?
I have electrospun cross-linked polystyrene fibres on a glass substrate. I am looking for chemical or physical methods to generate porosity (to increase its surface area).
Thank You.
I have carried out quaternization of PVA for antimicrobial activity of the polymer. The sequence of reaction is PVA preparation followed by addition of KOH and then quaternary ammonium salt. Washed with anhydrous ethanol. First time at 0.1 g PVA the precipitates were formed. But at 1 g PVA, after 3 hrs of adding quaternary ammonium salt the curds were formed before the addition of ethanol. Molar ratio of PVA to QAS is 1:2.
Is it due to self-condensation?
Thanks for your response.
Only glass tubes can be used for Diethyl ether as far as I know. But i'm not sure how safe it is to centrifuge fish eggs in diethyl ether at 10000g. This is a step in the cortisol extraction.
Hi everyone,
I am PhD student and the area of my research work is Biomaterials. I am looking for a supervisor for Erasmus Exchange Scholarship Program for a year.
The main area of my research is preparation of nanocomposite membranes and their applications. Can you please suggest me supervisor from European region with research facilities like cell lines and animal model?
Thank you so much for your response.
I am looking for a literature demonstration of the cornerstone understanding that quantitatively links adsorbed protein composition to a measured biological response to a materials surface.
Could this be possible based on the nature of the biological material or preprocessing condition, such as osmotic dehydration? Because normally samples with higher effective moisture diffusivity should have faster drying rate.
in some journals it is given as density increases? is it rite
Need to do strain controlled fatigue test on ASTM E606 specimen. What I have referred from a research literature they have given strain ratio R, of 0.5 and a frequency of 0.2 Hz and strain levels of 0.7%–3.0%, 70% decline of peak load could anyone tell me how to decide this values ? From where they used the values there is no reference paper they have used in that particular research article.
I want to know what is the required or the enough period to check the cytocompatibility of the decellularized biomaterials to the seeded stem cells
For example, DNA has functional groups such as methyl groups and nanoparticles can interact with them and alter them. I was wondering if we have any technique by which we can quantify them or maybe do a qualitative analysis. Any leads would be great, Thank :)
hello every body
I am a PhD student in biomaterial course. what kind of job or carrier I can do after I graduate?
do not hesitate to express any idea about self-employment or doing for others.
I am specifically aiming to study the nanomaterial-nucleus interaction and having a hard time finding good softwares which can help me in the same. Any suggestions would be helpful. Thanks.
Kindly share the paper and method too. Thank you.
Dear everyone
I will process a biological material (polychaetes) to perform SEM. I am in doubt if after dehydration in alcohol the specimens can be stored in absolute alcohol for later drying at a critical point , and how long can I store the material for?
Best regard
Looking for small diameter wire that could be used to pattern channels within a biomaterial.
Dear colleagues,
I am a researcher working with bovine hydroxyapatite. I want to reduce the percentage of carbonate on the sample. Is there any suggested techniques for this? I am measured the carbonate from EDX (SEM-EDX). Thank you
Thank you.
As i know , if we can realize the DNA structure , we can simulate it in computer . then we can try to rebuild it if possible .
so Given the technological progress, is it possible in the future?
I am working with polyamine surface coating on bio-materials. I have seen in literature that microBCA detects the primary amine in proteins. Similarly, mostly peoples are using it for Dopamin's catechole amine estimation. I am wonder that the same way can I use microBCA for detection of polyamines coated on surface. However, I couldn't find the relevant literature.. I have checked my polyamine changes the color with microBCA and detected their OD values after coating but I am not sure this will be proper to show or not......
Your feedbacks will be highly appreciated
Thanks in advance
~Taufiq
Hi everyone, l have a question.
I made cell cultivation on a biomaterial containing chitosan and gelatin, fixed my cell culture samples on the 14th day with 4% paraformaldehyde and performed H&E staining. However, while the scaffold has cross-sectional images, the cell is not in the environment. why could this be caused?
Note: there is a picture attached.
Thanks.

can anyone help me about the standards?
I mean the mass of the biomaterial and volume of the PBS in one vial.
Main characteristic feature of hydrogel is the capable of holding large amounts of water in their three-dimensional networks. For nanoemulgel which known as the formulation of nanoemulsion based on hydrogel system and the medium is aqueous. Please explain me what is the similarity and dissimilarities between nanoemulgel and hydrogel?
Phalloidin stained filaments and dapi stained nucleus
Dear Researchers,
Please, which papers, sites, tools, or programs you recommend to use in the design and selection of the best materials for prosthetic leg liners?
Regards,
Akram
I would like to know what are the protocols, assays and theory to study a nanomaterial interaction with cancer stem cells (and not normal cancer cells).
In investigating collagen I got quite puzzled. Could you please offer me some advice? Here are some of my questions
1. In heterogenous tissue engineering, collagen seems to be potentially the backbone material, as PCL and hydroxyapatite for osteo-regeneration. How is the comparison of collagen with other alternatives (Poly(ethylene glycol), fibronectin, other protein and synthesized peptide like BiogelX)?
2. For the core issue in collagen biomaterial study, actually existed crosslinker (EDC/NHS, genepin, TG2) already meets most basic needs (stability, mechanics, cytotoxicity), aren’t they? The core issue has swifted to realise reconstruction of better microenvironment of different tissue, has it?
3. What are the critical requirements for collagen product (stability/rentation of triple helix, chain length, bound water, solubility and ?)? and how are the influences of these properties on biomaterial application? (swelling, porosity, mechanic property and fidelity, immuno-rejection, biodegradation and etc)
Thank you very much!
Some studies point that crosslinking increase the inflammatory reaction of collagen[1-3]. while some other studies suggests that Cross-linking also can be introduced to reduce the antigenicity. The cross-link formation can shield or modify major antigenic sites (tellopeptide) and, thus, reduce their capacity to interact with antibodies[4].
So does crosslinking actually increase or decrease the antigenicity of collagen as biomaterial?
1. Badylak SF. Decellularized allogeneic and xenogeneic tissue as a bioscaffold for regenerative medicine: factors that influence the host response. Ann Biomed Eng. 2014;42:1517–27. https ://doi.org/10.1007/s1043 9-013-0963-7.
2. Rothamel D, Schwarz F, Sager M, Herten M, Sculean A, Becker J. Biodegradation of differently cross-linked collagen membranes: an experimental study in the rat. Clin Oral Implants Res. 2005;16:369–78. https ://doi.org/10.111 1/j.1600-0501.2005.01108 .x.
3. Schwarz F, Rothamel D, Herten M, Sager M, Becker J. Angiogenesis pattern of native and cross-linked collagen membranes: an immunohistochemical study in the rat. Clin Oral Implants Res. 2006;17:403–9. https ://doi.org/10.1
111/j.1600-0501.2005.01225 .x.
4. Chevallay, B.; Herbage, D. Collagen-based biomaterials as 3D scaffold for cell cultures: Applications for tissue engineering and gene therapy. Med. Biol. Eng. Comput. 2000, 38, 211–218. [CrossRef] [PubMed]
Composition of Silica in composite bio materials
Dear All,
I'm looking for substance, probably 4-CMC analogue (precursor/metabolite). The product ion scan on ESI-UHPLC-QqQ-MS/MS is: 214>196>181>168>151>130>125>115>103>91>56 m/z (the highest intensitivity is 196 and 115 m/z) and probably the isotope: 216>198>183>170>155>130>127>115>91>56 m/z (the highest intensitivity is 198 and 115 m/z).
Ions are only in biological materials, not in evidences.
Thank you for any advice!
Regards,
Karolina Nowak
Is there any difference between the peptide bonds of fish gelatin, porcine and bovine gelatin?
Hello. I am currently working on a project which involves silk fibroin. The problem is , I'm facing gelation in different steps almost each time. I made 4 attempts till today. At the first attempt I was able to obtain %8 silk fibroin but on other 3 attempts I faced gelation problems twice at dialysis stage and once at autoclave. I'm sharing the protocol which I use. I hope you can give me some advice on the issue.
1. Prepare 5 grams of cocoon shell on a petri dish. (Cut them into small pieces)
2. Add 4.24 mg NaCO3 in 2 liters distilled water, dissolve the NaCO3 and heat the solution to 90 celcius degrees.
3. Add the cocoons to the hot distilled water and wait 30 minutes while stirring.
4. Take out the silk and put into a new beaker inside 2 liters of distilled water (at room temperature) for washing.
5. Wash at least 3 times for 20 minutes. (I'm washing it 4 to 6 times and I rinse the silk with distilled water with squeezing it between water changes) (I make sure that the soapy feeling is lost)
6. Put the silk into a petri dish.
7. Let the silk dry at 37 C for 24/36 hours.
8. Take the silk out and weigh it. Cut the dry silk into pieces.
9. Prepare lithium bromide (9.3M) solution and put 4x of the silk's weight:
-The amount of LiBr: [(86.65x9.3)/1000]x4x(Silk's weight)
-The amount of distilled water: 4x(Silk's weight)
10. Dissolve LiBr in distilled water with magnetic stirrer and add it onto the silk in a 50mL beaker.
11. Put the solution into 60 C incubator for 4 hours. (I waited 4 hours for first 3 attempts, then I waited 6 hours at my final attempt which solidified while autoclaving)
12. Put the liquid solution into dialysis sacks ( Sigma-Aldrich D6191-25EA; 12,000 Da MWCO) and tie the sack from up and down. Leave a little space on top.
13. Hang the dialysis sack in 2 liters of distilled water and open the stirrer.
14. Change the water in regular intervals (after 1h, 3h, 6h, 10, 20h) and rinse the LiBr for 2 days.
15. Open the sack and put silk fibroin into a glass bottle.
16. Autoclave.
17. Take 1 mL of autoclaved silk fibroin and put into a centrifuge tube without closing the cap. (Note the weight of centrifuge tube and the silk fibroin)
18. Put the tube into the incubator (60 C degrees) overnight.
19. Weigh the tube. Calculate the amount of dry matter and then calculate the percentage of silk fibroin.
Details:
-The lab's temperature is around 26 C degrees.
- I use different stirrers to avoid heating the water at step 5.
-The liquid I obtain after LiBr application is very viscous compared to the videos I watched.
-The cocoons are present at the lab for 4 years and they are being kept in room temperature in a drawer inside a plastic bag.
Hi,
I have UltiMate 3000 GPC working on methylene chloride. I would like to change solvent to THF for analyzing glycerol polyesters (not soluble in methylene chloride). My poliesters have molecular weight about 1-5kDa, maybe I could reach in future 30kDa.I need help with right choosing pre column, column and also correct method and standard substances for calibration. I don't have any experience with GPC. Thanks for help.
How biological materials (Animal carcasses, excrements) collected in field can be best stored for a later DNA and RNA extraction? what is the protocol to follwo and what are the storing equipements needed.?
3D printing in many settings is indicated to be a 'hobby'. However, if one envisions how 3D printing can contribute to re-shaping our day to day lives, one possibility is to make use of materials for common uses or that contribute to circular use cycles. Organic materials are available in many forms around our collective living environments. Therefore, understanding what materials are available and common products that are used could help to identify paths of research that could utilize 3D printing on larger scales and more common settings.
We are trying to evaluate tje host response to implantation of a biomaterial in a tissue defect.
So the antibodies should work on paraffin tissue sections
I'm trying to make PCL aligned fibers.
I've tried to adjust voltage, flow rate and needle-collector distance.
Parameters :
- 15% PCL in acetone (weight/volume)
- Needle-collector distance: 5 to 20 cm
- Voltage: 5 to 20 kV
- Flow rate : 1 to 10 mL/h
- Rotating speed: 500 to 2400 rpm
I manage to get fibers (at the lowest rotation speed) but they are not aligned.
I've tried to reduce flow rate, but still the rotation speed is too low and thus fibers are not aligned enough.
Above 500 rpm I can't manage to get a Taylor cone, and the droplet/jet constantly moves and solidifies.
I've read publications using similar parameters to mine and getting aligned fibers, what am I missing ?
why bio-material should not be crystalline in nature ? why we try to make sample amorphous for tissue engineering application
I have received the results from LCMS/MS instrument as intensity, ion number per second (cps). I have also similar data for my standards in the instrument. I could not use internal standard because I have to test biological material (fungi). How can I calculate analyte concentration?
If you send me a formula.
There are some analytical programs and numerical modeling software, including COMSOL, 3DSIM, ABAQUS, MARC, ANSYS (using stress analyses), and many other 3D or finite element based methods, which have already been used to find a relation between the parameters of AM processes (e.g. hatch distance, scan speed, and device power) and the properties of final products. It seems that previous researchers could already partially simulate some characteristics of manufactured products by the help of design of experiments/DOE methods (e.g. TAGUCHI) as well as microstructural studies (considering droplet geometry, phases characteristics, grain size, defects, etc.), thermal evaluations (CTE, heat capacity, conductivity, molten pool temperature distribution, etc.), or stress analyses. However, there is still no comprehensive method to precisely evaluate the influence of AM parameters and also the magnitude of their impact on the mechanical properties of the products.
Please let me know what method you think is the most complete, practical, and reliable approach which can best simulate the final properties of the additively-manufactured products using the process parameters.
Hi everyone. I would like to ask your opinion about storage conditions of biological material such as blood parts (platelet lysate). Our ultra freezer has dropped its temperature due to power loss from -80 to -60 for about 12 hours. Do you think this could affect stored biological material such as blood parts i.e platelet lysate?
Thank you in advance.
For my master's thesis, I grow cells on nano/micro-structured surfaces. I have some questions regarding the hydrophilic/hydrophobic nature of cell growth substrates.
I have read that some surfaces, such as pillars, have hydrophobic properties (as shown by water contact angle measurements).
What is the idea behind measuring the water contact angle of surfaces of which topography is changed (but not chemistry)?
For the cells, is the hydrophobicity of the substrate coming from topography change, the same as the hydrophobicity coming from chemical modifications (as for example, coating with hydrophobic molecules)?
For example, is the protein adsorption affected differently?
I am doing surface modification of polymer films using plasma in order to improve anti bacterial property.
Thickness of polymer film is 60 micron to 1 mm for different polymers.
I want to test it against E-Coli species.
What are the testing methods suitable to such substrates?
This work is in the direction of developing antimicrobial catheter surfaces.
Thank you in advance.
With kind regards,
Purvi
We can use SPD processes for producing products with many applications. One of them is the Bio application. Do you know about these processes applications in Biomaterials?
Hi! I am curious about the perspectives of bioresorbable Fe-based biomaterials. Are you aware of its real applications in medicine? What are the key issues to be solved before it comes to the market?
Do explain in term of BET based parameters such as BET Surface Area, Langmuir surface area, Adsorption-desorption volume of pores and pore diameter.
For our ongoing research project " Research on Biomaterials" we need a bulk amount of collagen.
for my research am in search of better biomateril to be used for drug delivery to eye
it has to be bio compatible,
need to be in hydrogel form
transparent
Hey just wondering if anyone has any experience with synthesising 58S bioglass as I'm running into the same problem each time I try. When I'm adding the CaN to the H2O, HCl, TEOS and TEP solution it solidifies to a waxy consistency before I've even added about a tenth of it. I've tried adding the CaN very slowly, or adding more HCl at the beginning etc . I have to synthesise the bioglass for a research project I'm doing as part of my integrated masters degree but I've not studied chemistry or done any chemistry experiments since high school so am pretty lost (I am studying Biomedical Engineering). Any help or pointers welcome. I plan to create different combinations of 58S Bioglass by replacing the Calcium with increasing levels of Strontium. I can provide more experimental details if required. Thanks in advance!