Science topic
Bioluminescence - Science topic
Explore the latest publications in Bioluminescence, and find Bioluminescence experts.
Publications related to Bioluminescence (10,000)
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The serine/threonine protein kinase CK2, a tetramer composed of a regulatory dimer (CK2β2) bound to two catalytic subunits CK2α, is a well‐established therapeutic target for various pathologies, including cancer and viral infections. Several types of CK2 inhibitors have been developed, including inhibitors that bind to the catalytic ATP‐site, bival...
Bioterrorism also called biological attack, refers to the deliberate release of microbes with the potential to cause illness or death in humans, animals, or crops. These weapons can trigger large-scale epidemics with unprecedented lethality and both nation-states and terrorist organizations have employed them for devastating consequences. Medical p...
The occurrence and severity of periodontitis (PD) tend to increase with age, and yet the underlying mechanisms remain unclear. Immune senescence is known to be triggered in mice and humans as they age. Experimental PD in mice has been shown to induce senescence biomarkers p16 INK4a and p21, dysfunction of antigen-presenting cells (APCs), and activa...
This study investigated Rhodamine B (RhB) degradation by electro-Fenton (EF), anodic oxidation (AO), and their combination (EF/AO), using a carbon felt cathode coupled to a sub-stoichiometric titanium dioxide Magnéli phase (Ti4O7) anode or a platinized titanium (Ti/Pt) anode. The results indicated that operational parameters influenced the kinetics...
Oral transmission of parasites via environmentally resistant cyst stages in contaminated food or water is a common route of human infection, but there are no effective vaccines available for any enteric parasitic infection. Our knowledge of parasite cyst stage conversion and interaction with the intestinal tract is limited. Here, we investigate inf...
Plant‐pathogenic bacteria colonise their hosts using various strategies, exploiting both natural openings and wounds in leaves and roots. The vascular pathogen Xanthomonas campestris pv. campestris (Xcc) enters its host through hydathodes, organs at the leaf margin involved in guttation. Subsequently, Xcc breaches the hydathode–xylem barrier and pr...
Many marine organisms feature sensitive sensory‐perceptual systems to sense the surrounding environment and respond to disturbance with intense bioluminescence. However, it remains a great challenge to develop artificial materials that can sense external disturbance and simultaneously activate intense luminescence, although such materials are attra...
Cardiomyopathy is the most common pathology associated with Trypanosoma cruzi infection. Reports that statins have both cardioprotective and trypanocidal activity have generated interest in their potential as a therapeutic treatment. Using a highly-sensitive bioluminescent mouse model, we show that 5 days treatment with statins has no significant i...
Bioluminescent bacteria play a crucial role in marine ecosystems, often forming symbiotic associations with marine organisms. In the present study, Photobacterium leiognathi, a bioluminescent bacterium, was isolated and identified from marine squid. A total of 30 samples were collected, and initial screening revealed nine non-luminescent isolates a...
The high morbidity and mortality of colorectal cancer (CRC) is a major challenge in clinical practice. Although a series of alternative research models of CRC have been developed, appropriate orthotopic animal models that reproduce the specific clinical response as well as pathophysiological immune features of CRC are still lacking. In the current...
The mammalian cryptochrome proteins (CRY1 and CRY2) are transcriptional repressors most notable for their role in circadian transcriptional feedback. Not all circadian rhythms depend on CRY proteins, however, and the CRY proteins are promiscuous interactors that also regulate many other processes. In cells with chronic CRY deficiency, protein homeo...
New faster and more sensitive detection methods are required for food microbiological contamination quality control throughout the entire handling process. The adenosine triphosphate (ATP) bioluminescence detection method based on the firefly luciferin–luciferase system has been a widely used approach for decades due to its practicality, efficiency...
This study provides a molecular and phenotypic characterization of an entomopathogenic nematode-bacterium complex isolated from agricultural soil and nematode efficacy against Helicoverpa armigera. The nematode, identified as Steinernema anantnagense KP_CU, was characterized using the internal transcribed spacer (ITS) region of rRNA, revealing 100%...
Background
Chordoma is a slow-growing, primary malignant bone tumor that arises from notochordal tissue in the midline of the axial skeleton. Surgical excision with negative margins is the mainstay of treatment, but high local recurrence rates are reported even with negative margins. High-dose radiation therapy (RT), such as with proton or carbon i...
Succinate is a pivotal tricarboxylic acid cycle metabolite but also specifically activates the Gi‐ and Gq‐coupled succinate receptor 1 (SUCNR1). Contradictory roles of succinate and succinate‐SUCNR1 signaling include reports about its anti‐ or pro‐inflammatory effects. The link between cellular metabolism and localization‐dependent SUCNR1 signaling...
The highest unqualified cleaning rate of suction-type lumen instruments is a major challenge for a central sterile supply department (CSSD). However, A few comprehensive studies have analyzed the main factors affecting cleaning quality. In response, this study aimed to explore the current state and the factors affecting the cleaning quality of reus...
Background and purpose
Bone metastasis is common for breast cancer and associated with poor prognosis. Currently, radiotherapy (RT) serves as the standard treatment for patients exhibiting symptoms of bone metastasis to alleviate pain. Whether earlier application of RT will better control bone metastasis remains unclear.
Methods
We utilized a mous...
Background
During their nighttime shoaling, the flashlight fish Anomalops katoptron produce fascinating, bioluminescent blink patterns, which have been related to the localization of food, determination of nearest neighbor distance, and initiation of the shoal’s movement direction. Information transfer e.g., via alarm signals is an important aspect...
Background
In vivo evaluations of the antimicrobial efficacy of biomaterials often use bioluminescent imaging modalities based on bioluminescent bacteria to allow follow-up in single animals. Bioluminescence production by bacteria is dependent on their metabolic activity. It is well known that several factors can influence the metabolism of bacteri...
Controlling and maintaining the cleanliness of the working environment in restaurants is crucial. The aim of this study was to determine the level of contamination on food contact surfaces in restaurants. A total of 28 restaurants were surveyed, and 500 samples were taken from food contact surfaces that appeared clean and ready for use. The surface...
Functional studies of host-microbe interactions benefit from natural model systems that enable exploration of molecular mechanisms at the host-microbe interface. Bioluminescent Vibrio fischeri colonize the light organ of the Hawaiian bobtail squid, Euprymna scolopes , and this binary model has enabled advances in understanding host-microbe communic...
Fatty acids (FAs) are essential building blocks for all the domains of life, of which bacterial de novo synthesis, called type II FA synthesis (FAS II), is energetically expensive. The recycling of exogenous FAs (eFAs) partially relieves the FAS II demand and, therefore, compromises the efficacy of FAS II-directed antimicrobials. The versatile acyl...
The click beetles (Elateridae) represent the major and well-known group of the polyphagan superfamily Elateroidea. Despite a relatively rich fossil record of Mesozoic Elateridae, only a few species are described from the Upper Cretaceous Burmese amber. Although Elateridae spend most of their lives as larvae, our knowledge on immature stages of this...
Background
The association between the intestinal microbiota and colorectal cancer (CRC) has been extensively studied, with Fusobacterium nucleatum ( F. nucleatum , FN) being found in high abundance in colorectal cancer tissues. Previous research has emphasized the significant role of F. nucleatum in the occurrence of CRC. However, the impact of F....
Introduction
Intestinal lymphoma may be latent in some dogs with chronic inflammatory enteropathy. Mesenchymal stromal cells (MSCs) have potential therapeutic applications for refractory chronic inflammatory enteropathy, but their impact on the development of potential intestinal lymphomas has not yet been evaluated. Therefore, this study was perfo...
Background
Cancer immunotherapy using immune checkpoint blockade (ICB) has revolutionized cancer treatment. However, patients with multiple myeloma (MM) rarely respond to ICB. Accumulating evidence indicates that the complicated tumor microenvironment (TME) significantly impacts the efficacy of ICB therapy. Therefore, investigating how TME componen...
Firefly luciferases have been extensively used for bioanalytical applications, including their use as bioluminescent reporters, biosensors, and for bioimaging biological and pathological processes. Due to their intrinsic pH- sensitivity, in recent years we have demonstrated that firefly luciferases can also be harnessed as color- tuning sensors of...
Antibiotic resistance stands as the foremost post-pandemic threat to public health. The urgent need for new, effective antibacterial treatments is evident. Protein-protein interactions (PPIs), owing to their pivotal role in microbial physiology, emerge as novel and attractive targets. Particularly promising is the α-subunit/β-sliding clamp interact...
The fungal genus Omphalotus is noted for its bioluminescence and the production of biologically active secondary metabolites. We isolated 47 fungal strains of Omphalotus olivascens germinated from spores of a single mushroom. We first noted a high degree of variation in the outward appearances in radial growth and pigmentation among the cultures. R...
All known bioluminescent fungi are basidiomycetes belonging to the Agaricales. They emit 520–530 nm wavelength light 24 h per day in a circadian rhythm. The number of known bioluminescent fungi has more than doubled in the past 15 years from 64 to 132 species. We currently recognize five distinct lineages of bioluminescent Agaricales belonging to t...
Quorum sensing (QS) system in bacteria, mediated by a small signaling molecule called an autoinducer (AI), promotes biofilm formation and other virulence factors that contribute to reducing the susceptibility of treatment agents. Escherichia coli is considered bacteria contaminated in the food industry with their biofilm-forming ability which allow...
The bioluminescent European brittle star Amphiura filiformis produces blue light at the arm-spine level thanks to a biochemical reaction involving coelenterazine as substrate and a Renilla-like luciferase as an enzyme. This echinoderm light production depends on a trophic acquisition of the coelenterazine substrate. Without an exogenous supply of c...
Renewable resource use and sustainable designs are gaining importance for electrically sourced artificial lighting. At this point, the biomimicry approach offers innovative solutions to develop artificial systems, products, and processes inspired by nature. When the studies conducted in the field of biomimicry regarding lighting are examined, it is...
Campylobacter jejuni, a major cause of foodborne zoonotic infections worldwide, shows a paradoxical ability to survive despite its susceptibility to environmental and food-processing stressors. This resilience is likely due to the bacterium entering a viable but non-culturable state, often within biofilms, or even initiating biofilm formation as a...
The cyclic GMP-AMP synthase-stimulator of the interferon gene (cGAS-STING) signaling pathway is considered an essential pattern recognition and effector pathway in the natural immune system and is mainly responsible for recognizing DNA molecules present in the cytoplasm and activating downstream signaling pathways to generate type I interferons (IF...
Continued efforts to discover new antibacterial molecules are critical to achieve a robust pre-clinical pipeline for new antibiotics. Screening of compound or natural product extract libraries remains a widespread approach and can benefit from the development of whole cell assays that are robust, simple and versatile, and allow for high throughput...
Globally, prostate cancer is the second most common malignancy in males, with over 400 thousand men dying from the disease each year. A common treatment modality for localized prostate cancer is radiotherapy. However, up to half of high-risk patients can relapse with radiorecurrent prostate cancer, the aggressive clinical progression of which remai...
Salmonella enterica serotype Reading has recently been identified as a significant foodborne pathogen from contaminated poultry products. There is a critical need for close monitoring of this newly emerged pathogen. This study developed bioluminescent strains of S. Reading for real-time pathogen tracking using bioluminescence imaging. Two strains o...
The bioluminescence reaction of firefly luciferase with D-luciferin has become an indispensable imaging technique in modern biology and life science experiments, but the high cost of D-luciferin is limiting its further application. Here, we report a practical, one-pot synthesis of D-luciferin from p-benzoquinone (p-BQ), L-cysteine methyl ester and...
### tl'dr
A meta-cognitive exercise in humor generation, exploring the AI's capacity for abstract humor aligned with complex theoretical frameworks.
dyb interacts w AI personas to teach one of them Jerry humour from an abstract theoretical PoV
'"The punchline intentionally subverts our expectations by providing an absurd answer that has no logi...
Background: Circulating tumor cells and clusters (CTC) from soft-tissue sarcoma (STS) that become entrapped in the lung can form micro-metastases and lead to pulmonary metastatic disease. Many patients with localized high-risk STS later develop metastases. Radiation is effective at reducing local recurrence by eradicating microscopic infiltration a...
Pyrosomes represent a group of marine holozooplankton. They do not exist as individual entities but as intricate colonies comprising numerous zooids, well-known for their bioluminescent properties. Pyrosomes inhabit most oceanic waters spanning tropical to temperate regions. They serve as significant consumers of phytoplankton, playing a crucial ro...
We previously reported that mice immunized twice with a lipid nanoparticle vaccine comprising four monkeypox viral mRNAs raised neutralizing antibodies and antigen-specific T cells and were protected against a lethal intranasal challenge with vaccinia virus (VACV). Here we demonstrated that the mRNA vaccine also protects mice against intranasal and...
Os desreguladores endócrinos (DEs) são substâncias capazes de interferir no sistema endócrino dos seres vivos, sendo associados a consequências adversas tanto na biota quanto na saúde humana. Exemplos de DEs incluem pesticidas, fármacos, surfactantes e estrogênios (hormônios), encontrados em águas superficiais, subterrâneas e em estações de tratame...
The regulation of solution pH on the structural and optical properties of peptide self‐assemblies remains a critical yet unresolved issue in peptide research. This study investigates the heptapeptide Ac‐IHIHIQI‐NH2 and its intrinsic fluorescence across a range of pH levels, demonstrating that variations in pH lead to significant changes in the morp...
Biosensorics is a new branch of analytical biotechnology, one of the main directions of which is the development of electrochemical biosensors. Microorganisms capable of bioluminescence are often used in biosensor systems. Such microorganisms include marine bioluminescent bacteria Aliivibrio ficsheri, Vibrio campbellii, Vibrio harveyi, Photobacteri...
This review paper explores the realm of non-invasive methods for early cancer detection. Early identification is crucial for effective therapeutic intervention, and non-invasive techniques have emerged as promising tools to enhance diagnostic accuracy and improve patient outcomes. The paper thoroughly examines the advantages, limitations, and prosp...
The lunar cycle has a major influence on the daily structure of marine ecosystems. As the moon orbits the earth across an approximate 29.5 day cycle, it influences these systems by altering nighttime light availability and shaping the strength and timing of tides. Numerous studies have documented the effects of the lunar cycle on large epipelagic f...
Electrospun nanofibers offer a highly promising platform for the delivery of vaginal lactobacilli, providing an innovative approach to preventing and treating vaginal infections. To advance the application of nanofibers for the delivery of lactobacilli, tools for studying their safety and efficacy in vitro need to be established. In this study, flu...
Background
Bone is the most frequent site of metastasis for breast cancer (BC). Metastatic BC cells interact with bone cells, including osteoclasts and osteoblasts, creating a cancer niche where they seed and proliferate. MicroRNAs (miRNAs) are regulators of breast-to-bone metastasis progression. MiR-24-2-5p has previously been shown to have roles...
Background
Increasing yield will have major implications for vaccine manufacturers. Internal bacterial contamination of specific pathogen-free (SPF) eggs results in batches of vaccines being rejected and may negatively impact vaccine yield.
Objective
The objectives of this study were to analyze Germ-Free (GF) eggs microbiologically and evaluate th...
The reversal of phototaxis has been observed in a wide range of animal species. However, environmental chemicals that can cause a quick reversal of phototaxis have rarely been reported. Here we identified hypochlorous acid (HClO) as an inducer of phototactic reversal in Vargula hilgendorfii , also known as sea fireflies. This species shows innate p...
Background/Objectives: Anguillid herpesvirus 1 (AngHV-1) (recently renamed Cyvirus anguillidallo 1) is the etiologic agent of a lethal disease that affects several eel species. It is thought to be one of the main infectious agents causing a population decline in wild eels and economic loss within the eel aquaculture sector. To date, no vaccines are...
The brittle star Amphiura filiformis is a keystone species within the burrowing fauna assemblage in the fine, muddy sediments along the European coasts. With high regeneration and bioluminescence capabilities, it emerged as a model species for many studies. However, only a few studies have investigated the cellular components of its coelomic fluid,...
Circadian rhythms are important for maintaining homeostasis, from regulating physiological activities (e.g., sleep–wake cycle and cognitive performance) to cellular processes (e.g., cell cycle and DNA damage repair). Melatonin is a key regulator of circadian rhythms and exerts control by binding to melatonin receptor 1 (MT1), decreasing neuronal fi...
The paper presents the results of study on the luminescent system of the culture of the fungus Mycena gombakensis derived out of wood samples. The changes on samples stored for duration of 10 years in non-sterile conditions and the dynamics of luminescent signals are described. A phylogenetic approach was used to recognize fungal species. A number...
Background and Purpose
Orexins have important biological effects on the central and peripheral nervous systems. Their primary ability is to regulate the sleep–wake cycle. Orexins and their antagonists, via OX1 receptor have been shown to have proapoptotic and antitumor effects on various digestive cancers cell models. We investigated, (1) the abili...
Background
During mammalian gestation, fetal circadian rhythms are thought to be mainly controlled by maternal signals. In humans, the initiation and activity of central and peripheral circadian clocks is largely unknown. This study aimed to elucidate the developmental clock properties in human umbilical vein endothelial cells (HUVECs).
Methods
HU...