Biological Control Of Plant Diseases - Science topic
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Since my graduation in 2006 as an Agriculture graduate, I have been witnessing the importance and benefits of biological control agents (BCAs) over chemicals in the management of plant diseases but even after two decades, I could not observe a significant implementation of this very impactful approach even worldwide. I wish but in doubt, how come it will be possible......
Looking hopeful for a data based discussion. Thank you
I'm working on a proposal in which I wish to demonstrate that there is a gene that I can use in a virus or vector of transference that is able to confer resistance to the trees of citrus fruits against the Citrus tristeza virus.
Do you have experiences with alternatives to synthetic pyrethroids in tea pest control? Tea is a very often per year sprayed plant with synthetic pyrethroids against damaging insects. So insectizide resistance has become high and pestizide residues in tea are possible. Which good alternatives methodes are there? Best thanks for answers! J HUMER
Due to the high environmental risk of chemical pesticides, biological control of plant diseases with bio-pesticides are highly encouraged and recommend. Chitosan and oligochitosan are the well-known biological control agent for its nontoxic, biodegradable and biocompatible properties.
Good day, all. In a biocontrol study, we can easily determine disease severity by using a disease scale developed for a particular plant disease.
On the other hand, disease incidence measures the percentage of plants infected by the disease.
So, I have completed a glasshouse experiment for 84 days and I want to determine the disease incidence. However, based on the disease scale, all my plants treated with biocontrol agents show mild wilting symptoms whereas plants challenged with pathogen alone (control treatment) show complete wilting.
Based on the definition of disease incidence, this means my biocontrol-treated plants are infected (still show mild wilting) and the disease incidence would be 100% which is the same as the control treatment.
I am not entirely sure if disease incidence can be utilized as a parameter in a biocontrol study. Can someone help to clarify this?
Thank you in advance.
Agrobacterium bv1 with cucumopine Ri plasmid causes root mat disease of cucumber and tomato in greenhouses. I cannot find any efficient way to reduce the disease on plants and remove the pathogen from mats and hydroponic tubes.
I need your help on how to calculate CFU/g soil. I would greatly appreciate if you could check if my calculation is correct.
5 g of soil was placed into 50 mL water. The solution was serial diluted 10x (1/10). 0.1 mL of the dilution was spread onto the agar plate.
30 bacterial colonies were obtained.
5 g of soil was placed in 50 mL water so I have 100 mg soil in 1 mL of water.
With a dilution of 1/10, I have 10 mg of soil in 1mL of water
Next, I spread 0.1 mL of this dilution -> 1 mg of soil
I have obtained 30 CFU so I have 30 CFU on 1 mg of soil. Consequently, I have 30 000 CFU on 1 g of soil
Is this correct?
Thanks in advance.
Pomegranate trees, fruits, leaves, stems, all parts are spoiled, due to bacterial blight, and checked it is xanthomonas axonopodis, what will the permanent solution or treatment be?
I am going to be working with Bemisia tabaci management in green house condition. What may be your suggestion in regards of bio-rational or eco-friendly treatments (including botanicals, biopesticides, safer chemicals etc.) to be added in my experiment.
Hello everybody, wilt disease in tomato plants, in particular that caused by Fusarium oxysporum f. sp. lycopersici, is the most important disease which causes serious economic loss in all tomato growing areas of the world. The management of this disease still complicated as the biological control for plant pathogenic fungi can work well as long as the control organism targets only the invasive species. Since, all the methods used have some restrictions’ no single technique can manage the disease effectively. We are looking for sustainable approaches for management this disease
Dose anyone know a bout wood vinegar, which part of this liquid can kill the pests?
Hi i am looking for bioagent(fungi )active against Hyacinth water,a very destructive water weed.
I am planning to carry out bio-control on crown rot of banana. Now my question is what scale of disease measurement can i use.
Which protocol i may follow to preserve plant pathogens at -20 and at -80 degree Celsius specially Rhizoctonia, Alternaria, Bipolaris, Curvularia, Sclerotium, Colletotrichum and Fusarium? we are not using liquid nitrogen based preservation more.
Thanks in advance
Can anyone introduce a sound formula to calculate additive, synergism and antagonism between two toxins against pests (a formula with sound references)?
i want to know bacterial and fungal strains present on the surface of black gram(vigna mungo)
It's taken for granted that agricultural wastes are contaminated by plant pathogens and pesticides. I would like to know the different techniques and methods you suggest to improve the supressiveness effect of composting against plant pathogens.
thank you and best regards
Different places/location have differing water pH. Do they have a direct effect on the efficacy of the biopecticide?
During production of bio-control agents such as metarhizium, trichoderma, there is addition of yeast which forms a major contamint at the end.Why is it added and it is not anaerobic fermentation as oxyg. is supplied?
We have many problems with spiroplasma and maize rayado fino in corn seeds production areas in central Brazil and did not find an efficient vector control.
These parameters shall be used for the in vivo evaluation of the effect of Citronella grass essential oil on Fusarium.
I found attached symptoms in 35 days old ricebean plants. The leaf started bit drying from margin followed by upward curling. Later on full curling and complete drying. Ultimately, total plant dries. I found, the symptoms are covering whole field within 3-4 days. It has not affected nearby Dolichos. Interestingly, initially 1-2 leaves showing symptom and other leaves normal, slowly covering all leaves.
What is the best way to keep long yellow rust uredospores?
While working on root-knot nematode's hatching rate, there are some isolates' egg-mass that did not hatch in some treatments while others did. We want to know if it is the effect of the treatment or something else. Is there anybody that could help with explaining egg-mass chemical structure and physiology?
Also, any information on the egg's and nematode's cell walls and molting physiology?
Does the availability of O2 affect the egg hatching? What affects the egg-mass?
Thank you so much in advance.
With respect to the botanicals, biological agents, and the chemicals.
I'm working with walnut rootstock. I noticed a lot of plants have white streaks/fuzzy white clouds around the callus. They made the plants grow slowly and made the callus turn dark brown/black. Sometimes, no callus form at all. Could you guys suggest some ways to control it? Would PPM or streptomycin help? Thanks for any thoughts.
Can anyone tell me what I could see from the value of N, P, K and Zn, pH to infer the change of available nutrients to plants or uptake of nutrients, etc? Please see the attachment.
Invasive species, most of which are exotic, have become significant threat to our environment, economy, health and well-being. The best way of control is to prevent establishment in the first place by adopting prompt eradication action. However, if an invasive species is established, options like chemical control or mechanical excavation for removal can cause environmental damage. Target specific biological control can work well; otherwise there is a risk that the control organism might also become an invasive species. Alternative, such as manipulating existing natural enemies and/or the environment to enhance biological control, are also on hand. Despite all these tackling the problem of invasive species remained a great challenge. Hence what should be the sustainable solution and practicable strategy to deal with the continually growing problem of invasive species?
I am designing a guide RNA for Crispr to target specific genes in rice that when silenced can have a good effect on the stability of rice, such as disease-resistance.
Erwinia amylovora is a new pathogen for Russia and other countries of the FSU. In Kazakhstan, about 60% of apple orchards are infected. But, in Russia, with cold climate, there are some evidence that the pathogen slows down, and infected trees stay alive for several years, without spreading disease to younger trees. Is there any examples on natural disappearing of the disease?
There is a possibility that due to some side effects of fungicide application certain active ingredients and potentially whole classes will be prohibited in future for use in agriculture. What are our options? What new resistant varieties, chemistries, biopesticides or biological control agents will be available for efficient disease control in cultivated plants? Which alternative approaches for plant protection will be pursued and employed to maintain the supply of raw food produce with the high standards of environmental protection?
I have read that intercropping is one of the approaches to control wilt diseases or dieback in a Guava tree. What about chemical controls?
I stumbled on a variety of papers from the nineties that claim the use of biological control agents against various diseases in Brazil and other subtropical regions. Since research of the underlying mechanisms of biocontrol is scarce for most traditionally used BCAs, I wonder if they are still in use today? Anyone up-to-date on what is currently used as BCA by farmers in warmer climates?
Pseudomonas syringae pv. syringae and pv. atrofaciens cause diseases of wheat and other cereals, most harmful at early spring on winter crops. It was a problem in the USA from 1960-70 (Otto, 1976, 1977).
What can we do to reduce the development of this disease?
I want to make protoplast fusion between different species of trichoderma.
To identify unique microbial strains a lot of methods have been studied, I want to know simple method to detect such strains.
Plant pathogens are very specific to their host . In the case of non host plant pathogen they will produce hypersensitive reaction like necrosis. If this is the case non host plant could be anything apart from the host. Then why is nicotiana tabacum alone used for performing that test?
I am working on the effects of fungi on the growth and biochemical aspects of forest trees.
Can anyone suggest me which hypothesis to test?
I know that these stimuli have to work together. I mean it is difficult to separate them and the bibliography about them is quite important. But if we combined in one experiment a relatively old plant, a virus infection and an aphid parasitoid, can these effects be additive? Virus infection sometimes changes the nutritional quality of a plant in order to beneficiate aphids. On the other hand, foraging behavior of parasitoids can increment aphid movement, but when the aphid is already parasitized, some aphid show lower movement capabilities. And an older plant has normally lower nutritional quality, inducing aphid to move out to more nutritional plants, but in presence of parasiotids, large plants may be a better place to hide from parasitization than younger ones, especially knowing that parasitoids females only expend a short period of time in each patch in order to increment their efficiency.
Are there any thoughts of these issues?
Fengycins also hasmany isoforms, i am intrested in Fengycin B, A, C, S, or any other
I have been working on quantifying genomic DNA extracted from leaf (sugar beet) which was inoculated with fungus. I am able to amplify sugar beet DNA while I am not able to amplify fungal DNA. The DNA from leaves were extracted 3, 5 and 7 days post inoculation. Now the strange part: I am able to amplify fungal DNA with a regular PCR but am unable to amplify fungal DNA in a qPCR (Taqman Probe). I would appreciate any comments, suggestions or any different protocol that I can follow.
I use CTAB method for DNA extraction and FAM and HEX probes for qPCR.
I am thinking about purchasing a Bioanalyzer 2100 or a QIAxcel Advanced System. Could anyone advice me which system is better/cheaper for use? Or if there is any alternative equipment which have less expensive consumables? Maybe a better idea would be to buy a regular CE machine? Our lab is starting a project which is aiming to develop a standardized protocol for multiplex detection of several potato viruses directly from tubers. We will be working in collaboration with other labs, and I was thinking that it would be cool to have a protocol based on the specific RIN value. However, I do not want to end up with equipment which is expensive in use and will be collecting dust after finishing this project. We do work also with proteins and DNA on other projects, and potentially I would have an application for a Bioanalyzer, especially in combination with OffGel 3100. But since I never had a chance to use a Bioanalyzer 2100 or QIAxcel, I am afraid that both may be too expensive to use in routine lab work.
I am concerned with the study of insecticidal efficacy of certain plant extracts, the plants chosen are mostly wild herbs and shrubs. I collect the plants for the experiment during their flowering stage, however recently it was suggested that it is important to determine the plant age before collection.
DNA does not amplify and it looks degraded on the gel
How to inoculate arbuscular mycorrhiza(AM) in pots for wheat crop.
During my exploration of the Saudi flora, I have found this Withania sominfera with a strange leaves colour, is it a fungal/bacterial or virus disease, dose this plant deserve a phytochemical study in order to isolate new secondary metabolites caused by this infection? Please note the spots as indicated with the red arrow.
Does anybody know any assay using extracts of a resistant plant extract to control disease in susceptible plants of the same species?