- Justin Mattam asked a question:NewSurface roughness measurement, which is better and faster laser scanning microscopy or white light interference microscopy?
I wanted to know which methodology is faster without much loss of data, white light intereference microscopy, or laser scanning microscopy? The target area is 2x2 sqmm ablated with laser creating surfaces with Rz ranging from 2,3microns to 26 microns. Any leads would be helpful
- Oliver Krohn added an answer:6Does anyone have experience with 3D reconstruction and volume rendering of confocal z-axiz stacks?
I'm analysing ER-mitochondria contact sites but I can not find a detailed protocol to obtain 3D reconstructed and surface rendered images with ImageJ.Thank youFollowing
- Chiranjib Banerjee added an answer:4How to immobilize a vesicle under optical microscope to get a good 3D image?
Thank you!!!!!!! Peter, David and Lira, all the techniques are very interesting and definitely it will help my research.Following
- R. Schirhagl added an answer:7Non luminescent particles in the batch and accounting for them in bioimaging?
In case of fluorescent nanocrystals not all crystals fluorescence. This can be seen when we do a EM with cathodoluminescence that there are some crystals that dont emit while in fluorescence we dont see all the crystals and only ones that emit. If we are quantifying uptake using luminescence how does one account for the particles that dont luminesce?
You can look at the particles on a slide and determine the percentage of particles that are fluorescent and then correct the number that you get from uptake as suggested above. You can also use irradiated diamonds then you can have batches where they all fluoresce. I think you might also be able to see diamonds with raman imaging.Following
- Peter Kapusta added an answer:1Do you think that QD blinking can affect biosensing applications when measuring fluorescence for detection of very diluted analytes??
Or is it just a problem for bioimaging and single molecule analysis or when performing ultra-fast measurements?
Thanks for any help!
QD blinking is not a problem. It is a well understood natural phenomenon. Whether it "affects biosensing applications when measuring fluorescence" depends on what that application is and what is the time scale of the actual measurement.Following
- Klaus Buttinger asked a question:OpenSuperb Microvascular Imaging (SMI): Is it a reliable tool for evaluating thyroid nodules?
I'm trying to identify suspicious lesions by using Superb Microvascular Imaging (SMI). Has anybody experience in this field?Following
- Bhushan Patil added an answer:2Is there any optical properties difference between Graphene quantum dots and N-doped graphene quantum dots?Actually, graphene quantum dots (GQD) show different optical properties. Is there any change in the optical property after doping with nitrogen in the GQD?
Recent studies shows after doping nitrogen fluorescence of graphene quantum dots is enhanced. see this paper
- Bruno Martin added an answer:4Which is the most widely image processing technique in biological images? Can anyone review it briefly, and also why it has been adopted widely?
Biological images mostly have blurred or low resolution. The challenge has been identifying a good technique in 2D and 3D processing. Please clarify it.
At last ICIP2015, Michael Unser gave a talk about "Challenges and Opportunities
in Biological Imaging". In his talk, he explains some of the current challenges of biological imaging and the techniques used to address them. Worth checking!Following
- Closed account added an answer:4How does the nanostructure of II-VI quantum dots determine their optoelectronic properties?
I am doing a presentation in which I address the structure-property correlation for quantum dots used in bioimaging and biolabeling. I have found several articles on CdSe quantum dots specifically, but I am looking to understand the bigger picture on a more general level. What are the consistent structural factors that affect the properties of all II-VI compound quantum dots?
you can see the attached linksFollowing
- Jayampathi Basnayake added an answer:5Is there any software for converting RGB fractional exposures to real wavelengths?
I am using an IDS camera to identify RGB color ranges on some diseased leaves. It is easy to pick up the fraction of RGB (out of 255 grades) but I need to convert this into real wavelengths. Any ideas or advice would be appreciated.
I found an interesting paper by Brian Smits; An RGB to Spectrum Conversion for reflectances. This is more practical approachFollowing
- David S Gibson added an answer:2Has any institution had issues with UVP imaging products?
We've had a few issues with our UVP imaging system which seems specific to our camera. I'm just curious if any other institutions have had similar issues with this brand?
Also problems connecting to camera; previous problems with lines across the CCD. Replacements do take some time which is not good for a busy lab.Following
- Vladimir Bobic added an answer:5Does anyone have any experience in imaging human Lamina Splendens?
The uppermost layer of hyaline articular cartilage is often called Lamina Splendens. This film-like layer is seldom seen arthroscopically and various optical and electron microscopic imaging studies have shown conflicting morphology and structure of articulating surface. Considering its location there is no doubt that this zone, whatever the name, has extremely important articular (tribological) function as it provides a very low friction lubrication surface and contains lubricant and collagen fibrils which run parallel to the surface of articulation. This surface zone layer is likely to play a key role in maintaining the mechanical response of articular cartilage to load and wear and tear
Thank you for your reply. Do you have any images of LS, which you would be happy to share?Following
- Yash Agarwal added an answer:9How do you convert DICOM images to a 3D solid model (iges, step, etc) which needs to be input into Abaqus for further analysis?
I currently have a set of 500 DICOM image of slides of a rhino's frontal snout and I am specifically interested in its frontal horn for further analysis.
I have tried a lot of software for the conversion but have had no success yet. It would be great if I could use any free software which does the above conversion directly.Following
- Dennis Tobias Klier added an answer:1What are the instruments and components are need for establish new Transient pump -prob or Transient Absorption Spectroscopy for bioimaging?
I need clear each and everything about instrumentation setup from basic alignment to final calibration of pump-prob spectrometer. I need all laser systems functional procedure and what are the safety measures to be taken and consider while in this experimental arrangements? Give some basics to advance ideas to setup this facility. Each and every aspects of ideas will be appreciated and make an excellent setup to be utilized many researchers for their research works.
Is it possible to give some more detailed information on the samples you are interessted in. Because there are so many different laser-detector systems combinations possible without a deeper insight what you are trying to achieve.Following
- Yoon-Soo Jang added an answer:3What's the working principle of micro channel plates (MCP) Photomultiplier tube (PMT)?
Why it's so special compared to traditional PMT? Why it can't respond to high repetition rate (tens of MHz) and need to be pulse picked to low down the repetition rate (~ tens of kHz)?
It takes a time to multiply photon in MCP.Following
- Cao Guoyang added an answer:7What is the refractive index of graphene oxide?
Refractive index of graphene oxide in thin film.
Hi！Did you have the detailed answer for your question? Could you share the answer for us? Thank you!Following
- M. Ricky Ramadhian added an answer:2How do you quantify fibrosis in an aorta wall with imageJ?I want to quantify fibrosis in an aorta wall with ImageJ but it seem difficult to analyze. I read there is journal to use polarization microscope, but in my laboratory there is no polarization microscope. Can ImageJ make images become polarization microscope?to adjust threshold,,,Following
- Sankalp Verma added an answer:6How can we prevent photobleaching in fluorescent labeled protein stock solution?My protein samples are labeled and are kept in dark at -80. I am afraid they might undergo photobleaching if kept for long.thanks...I guess my samples were degrading because of some other reason...Following