• Bruno Martin added an answer:
    Which is the most widely image processing technique in biological images? Can anyone review it briefly, and also why it has been adopted widely?

    Biological images mostly have blurred or low resolution. The challenge has been identifying a good technique in 2D and 3D processing. Please clarify it.

    Bruno Martin

    At last ICIP2015, Michael Unser gave a talk about "Challenges and Opportunities
    in Biological Imaging". In his talk, he explains some of the current challenges of biological imaging and the techniques used to address them.  Worth checking!

    + 1 more attachment

  • Saeideh Hosseini added an answer:
    How does the nanostructure of II-VI quantum dots determine their optoelectronic properties?

    I am doing a presentation in which I address the structure-property correlation for quantum dots used in bioimaging and biolabeling. I have found several articles on CdSe quantum dots specifically, but I am looking to understand the bigger picture on a more general level. What are the consistent structural factors that affect the properties of all II-VI compound quantum dots?

    Saeideh Hosseini

    you can see the attached links

    + 5 more attachments

  • Julien Gravier added an answer:
    Non luminescent particles in the batch and accounting for them in bioimaging?

    In case of fluorescent nanocrystals not all crystals fluorescence. This can be seen when we do a EM with cathodoluminescence that there are some crystals that dont emit while in fluorescence we dont see all the crystals and only ones that emit. If we are quantifying uptake using luminescence how does one account for the particles that dont luminesce?

    Julien Gravier

    I am afraid that there is no such paper or they must be relatively rare. As Thomas Nann pointed out, most of the time the assumption is made that dark and fluorescent nanoparticles have the same fate in vivo. To the best of my knowledge, fluorescent and radioactive nanoparticles are generally compared for in vivo biodistribution. At the cellular level however I guess your best bet would probably be to look for fluorescence + TEM papers. I'll let you know if I find any.

  • Jayampathi Basnayake added an answer:
    Is there any software for converting RGB fractional exposures to real wavelengths?

    I am using an IDS camera to identify RGB color ranges on some diseased leaves. It is easy to pick up the fraction of RGB (out of 255 grades) but I need to convert this into real wavelengths. Any ideas or advice would be appreciated.

    Jayampathi Basnayake

    I found an interesting paper by Brian Smits; An RGB to Spectrum Conversion for reflectances. This is more practical approach 

  • David S Gibson added an answer:
    Has any institution had issues with UVP imaging products?

    We've had a few issues with our UVP imaging system which seems specific to our camera. I'm just curious if any other institutions have had similar issues with this brand?

    David S Gibson

    Also problems connecting to camera; previous problems with lines across the CCD. Replacements do take some time which is not good for a busy lab.

  • Vladimir Bobic added an answer:
    Does anyone have any experience in imaging human Lamina Splendens?

    The uppermost layer of hyaline articular cartilage is often called Lamina Splendens. This film-like layer is seldom seen arthroscopically and various optical and electron microscopic imaging studies have shown conflicting morphology and structure of articulating surface. Considering its location there is no doubt that this zone, whatever the name, has extremely important articular (tribological) function as it provides a very low friction lubrication surface and contains lubricant and collagen fibrils which run parallel to the surface of articulation. This surface zone layer is likely to play a key role in maintaining the mechanical response of articular cartilage to load and wear and tear

    Vladimir Bobic

    Dr Elmesiry,

    Thank you for your reply. Do you have any images of LS, which you would be happy to share?

  • Yash Agarwal added an answer:
    How do you convert DICOM images to a 3D solid model (iges, step, etc) which needs to be input into Abaqus for further analysis?

    I currently have a set of 500 DICOM image of slides of a rhino's frontal snout and I am specifically interested in its frontal horn for further analysis.

    I have tried a lot of software for the conversion but have had no success yet. It would be great if I could use any free software which does the above conversion directly.

  • Olivia Harding added an answer:
    Does anyone have experience with 3D reconstruction and volume rendering of confocal z-axiz stacks?

    I'm analysing ER-mitochondria contact sites but I can not find a detailed protocol to obtain 3D reconstructed and surface rendered images with ImageJ.Thank you

    Olivia Harding

    If you have access to Imaris software, it provides very high quality 3D rendering for confocal images. Imaris also allows data analysis based on your 3D rendering parameters. 

  • Dennis Tobias Klier added an answer:
    What are the instruments and components are need for establish new Transient pump -prob or Transient Absorption Spectroscopy for bioimaging?

    I need clear each and everything about instrumentation setup from basic alignment  to final calibration of pump-prob spectrometer. I need all laser systems functional procedure and what are the safety measures to be taken and consider while in this experimental arrangements? Give some basics to advance ideas to setup this facility. Each and every aspects of ideas will be appreciated and make an excellent setup to be utilized many researchers  for their research works.

    Dennis Tobias Klier

    Is it possible to give some more detailed information on the samples you are interessted in. Because there are so many different laser-detector systems combinations possible without a deeper insight what you are trying to achieve.

  • Yoon-Soo Jang added an answer:
    What's the working principle of micro channel plates (MCP) Photomultiplier tube (PMT)?

    Why it's so special compared to traditional PMT? Why it can't respond to high repetition rate (tens of MHz) and need to be pulse picked to low down the repetition rate (~ tens of kHz)?

    Yoon-Soo Jang

    It takes a time to multiply photon in MCP.

  • Cao Guoyang added an answer:
    What is the refractive index of graphene oxide?

    Refractive index of graphene oxide in thin film.

    Cao Guoyang

    Hi!Did you have the detailed answer for your question? Could you share the answer for us? Thank you!

  • M. Ricky Ramadhian added an answer:
    How do you quantify fibrosis in an aorta wall with imageJ?
    I want to quantify fibrosis in an aorta wall with ImageJ but it seem difficult to analyze. I read there is journal to use polarization microscope, but in my laboratory there is no polarization microscope. Can ImageJ make images become polarization microscope?
    M. Ricky Ramadhian
    to adjust threshold,,,
  • Abhijeet Lale added an answer:
    Is there any optical properties difference between Graphene quantum dots and N-doped graphene quantum dots?
    Actually, graphene quantum dots (GQD) show different optical properties. Is there any change in the optical property after doping with nitrogen in the GQD?
    Abhijeet Lale
    Yes there is difference between optical properties of undoped GQD and N-doped GQD. The undoped GQD has a very high fluorescence while doped GQD have no detectable photo-luminescence. Please refer to paper by Q Li published in JACS. The following explanation is taken from the same.
    This is consistent with the absence of n→π* , the lowest-energy transitions, in
    the absorption spectra of doped GQD. The n→π* transitions are expected for the N-substituted aromatic heterocycles. The large size of doped GQD explains the diminishing oscillator strengths of the n→π* transitions, because of the decreasing orbital overlap between the increasingly delocalized π* electrons and the nonbonding electrons localized on the nitrogen atoms. In contrast, the lowest-energy transition in undoped GQD is π→π*, which is responsible for the emission and has a less size-dependent oscillator strength.
  • Arunas Ramanavicius added an answer:
    Any suggestions on resolving the "blinking" of quantum dots (CdSe/ZnS) for confocal microscopy?
    I am trying to image the distribution of quantum dots (QDs) in tissue using confocal laser scanning microscopy. I am having a hard time tracking them and it is well known that the blinking of QDs poses a problem in bioimaging. Does anyone have suggestions to resolve this issue?
    Arunas Ramanavicius
    You can encapsulate them within polymeric layr, one example is attache:
    • Source
      [Show abstract] [Hide abstract]
      ABSTRACT: Polypyrrole formation initiated by UV/VIS irradiation and stabilization of (CdSe)ZnS quantum dots is reported. Presented results demonstrate that UV/VIS irradiation is slowly destructing Q-dots that decreases quantum yield and shifts peak of photoluminescence (PL) spectra to short wavelength range. The same Q-dot solution under UV/VIS light irradiation in the presence of pyrrole is more stable, there is no PL band shifts only PL intensity of Q-dot decrease. UV/VIS induced Ppy formation process in the presence/absence of Q-dots dissolved in toluene and phosphate buffer saline (PBS) not containing Q-dots was investigated. The increase in the optical absorbance in the range of 400 nm-600 nm was exploited for the monitoring of polypyrrole formation process. Results obtained proved that in the presence of UV/VIS formed polypyrrole destruction of Q-dots is much slower if compared with bare Q-dots destruction under similar conditions. However, formed polypyrrole showed no positive effect on quantum yield of Q-dot, just the opposite-polypyrrole quenched Q-dot photoluminescence. Results presented and observation by other authors on pyrrole polymerization allow to predict that by UV/VIS induced polymerization formed polypyrrole is assembling polymeric layer on heterogeneous phase between Q-dot and toluene or water (in PBS solution). Established pi-pi conjugated chain of formed polypyrrole quenches photoluminescence of Q-dot because this polymer was formed in close proximity to Q-dot surface.
      Full-text · Article · Apr 2009 · Journal of Nanoscience and Nanotechnology
  • Sankalp Verma added an answer:
    How can we prevent photobleaching in fluorescent labeled protein stock solution?
    My protein samples are labeled and are kept in dark at -80. I am afraid they might undergo photobleaching if kept for long.
    Sankalp Verma
    thanks...I guess my samples were degrading because of some other reason...
  • Robert Göstl added an answer:
    Is it possible for a photochromic material to be both thermally reversible and photochemically reversible ?
    I am investigating a thermally reversible photochromic material, therefore, the back reaction is conducted in the dark. During normal room light conditions the forward reaction occurs readily (deep blue colour change). However, the back reaction also seems to occur by exposure to visible light (>490 nm). The back reaction is both thermally and photochemically induced.

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