Questions related to Bioengineering
may I ask about if there is any human gene expression only depends on core promoter,which no need upstream any enhance and regulator ?
As I am wondering, if there are some gene, they are in modulation function, which also they are in very upstream of signal for a cell, therefore their core promoter 's binding affinity is strong enough stablizing the transcription complex, and so no need enhancer and upstream regulatory protein .Thank you so much
I have some lists of gene IDs from multi species, I want to have their compiled FASTA format files for each species. it looks tedious to copy each accession and collect FASTA seqs.
Batch Entrez is giving me error, may be because the identifier is related to other database.
Logistics seeks both to address real-world problems and research questions and to unite terminological, conceptual and methodological research aspects. In the last 20 years, biological research has developed extremely powerful methods and tools for fundamental and applied research. There is furthermore an increasing desire to build integrative research platforms that combine interdisciplinary and multi-level data to the structural complexity of biological systems. How much attention do you give to logistics for your research plans? Do you think logistics supports research methods, innovation, quality, value, and impact as well as training and participation opportunities for students as well as for practitioners? If so how and if not why not.
Dear researchers, Can tell me about modern, rapid and reliable technique for isolating macromolecules from plant sample ?
Metabolic rewiring and epigenetic remodeling, which are closely linked and reciprocally regulate each other, are among the well-known cancer hallmarks. Studies have reported use of Onco-metabolites to metabolically reprogram the epigenetic of cancer. I was wondering what might be major limitations of such techniques?
I am looking for a research work that implemented an uncertainty or statistical framework to study the impact of the geometric parameters on the fracture response.
I appreciate any help.
Thank you in advance,
Heart Rate Variability is a well known and useful concept in Biomedical Engineering and Medical Sciences. Breath Rate is a lesser researched field and a newer measure Breath-Rate Variability is introduced recently to quantify meditation effect.
It is gaining attention of researchers as BRV has a number of novel applications. What could they be.
I know that this depends on the rheological factors of the medium, but if you know any, please let me know.
For example, DNA has functional groups such as methyl groups and nanoparticles can interact with them and alter them. I was wondering if we have any technique by which we can quantify them or maybe do a qualitative analysis. Any leads would be great, Thank :)
I would like to get some pointers as to how DST-INSPIRE faculty fellowship proposal needs to be written. If anyone has a sample copy or an approved (old but shareable) it'd be great to get some information about the format.
I need practical protocol in detail. I do not know when endogenous respiration reached. The concentration of oxygen changes during time, and the oxygen uptake rate is dissolved oxygen variation per time. This quantity (dissolve oxygen variation per time) changes During time and is not constant, so when can I measure exogenous and endogenous respiration rate correctly? How do I measure the endogenous respiration rate? I think when endogenous respiration was reached; the concentration of oxygen did not change. So how can I measure changes in oxygen during the time?
I'm doing a survey as part of an Audacious program (https://www.startupdunedin.nz/audacious), which essentially is a StartUp initiative at Otago University. I'm curious to understand what level of programming do biologists these days need during their day to day research.
For all the biologists out there here are some questions to start the discussion on this topic:
1) Have you done any programming till date? If so which language did you use and for what purpose?
2) How have to overcome programming limitations? For example, did you get the work done through bioinformaticians, or sought help from your programming friend, etc?
3) Have you used online biological databases for your research? If so, which one?
4) How much of artificial intelligence have you used in your research? Do you see AI potential in your current work?
If you have anything else to add, please feel free.
I am Yacob Mathai
I have discovered The Purpose of Temperature of fever and Reason,and why our body acts against Facts of Physics in fever, which is not known to medical science.
This discovery is a life saving discovery.
As a result of my discoveries and researches on fever, two of my abstracts have been selected worldwide in more than 275 conferences for Oral Presentation.
I was not able to attend majority of these scientific conferences due to lack of money and sponsorship.
I have no personal benefits from these discoveries, but I take it as a service for the wellness of mankind to avoid millions of deaths every year due to fever and unscientific treatments done to cure it.
Due to my poor financial status I have not been able to attend several scientific conferences and meetings which I have been invited since 2006.
Review Article published in Advances in Bioengineering & Biomedical Science Research journal (Adv Bioeng Biomed Sci Res, 2018 Volume 1Issue 1) and Mini Review published in Journal of Nursing & Healthcare (JNur Healthcare, 2018 Volume 3, Issue 3, ISSN 2475-529X)
I was a keynote speaker in 4 prestigious international medical conferences.
1. 23rd Annual Congress on Paediatrics & Neonatology, November 05-06, 2018
2. 7th International Conference on Public Health and Community Nursing,
September 19-20, 2018 Singapore.
3. 2nd Global Summit on Paediatrics & Neonatology on June 25-26, 2018, Kuala Lumpur, Malaysia.
4. World Immunology Congress-December 14-15 ,2017 Dubai, UAE
The participated delegates found the Fever hypothesis presented by myself as the
most logical and analytical method for understanding and treating fever.
I am not a medically qualified person.But I have so many papers about fever,Inflammation and Back pain .In this situation How I will get travel grants.
I measured optical density using Bioscreen C (labsystems)
I inoculated sludge from sewage treatment plant to the 3 different substrates and added nutrients. And I found some problems in my data..
At the whole wells, the OD increased sharply simultaneously, and decreased after the log phase.
As I know, when the bacteria stop growing, the OD values are maintained, but not decreases.
Is this kind of phenomenon can happen?
Interested in knowing molecules with two or more sulfonic/phosphonic acid groups without a common endpoint (excluding phytic acid) and are available from natural resources or utmost commercially available.
Any relevant reading suggestions are also much appreciated. Thanks.
I am specifically aiming to study the nanomaterial-nucleus interaction and having a hard time finding good softwares which can help me in the same. Any suggestions would be helpful. Thanks.
According to Kokubo's instuction for preparing SBF (Simulated Body Fluid) pH should be increased from 2.0 to 7.4 after adding Tris to the solution. I added all additives one by one, and the pH became 1.8-1.9 right before adding Tris. But then after adding Tris it didn't increased.
Does it mean the Tris composition is wrong? or Should I consider something especial in adding Tris?
(The Kukobo's paper has been attached.)
Thank you so much in advance for your replies.
Does the term "contrast bath" imply any random time-temperature combination of hot and cold water or it is a specific combination of repetitive alternate spells of dipping the affected body part in cold water at a specific temperature for a specified duration followed by dipping in hot water at a specific temperature for a specified duration?
Glass microfiber filter is chemically stable, stable at 500ºC, mechanical strength etc.,. wouldn't that make glass microfiber unsuitable for VSS. While Ashless filter paper leaves no ash after firing at 500ºC. which one of these is the ideal one.
Kindly provide the details about this as it is a bit confusing matter.
We know HRT of fresh cow manure to produce biogas is more than 25 days(Ambient temperature around 30 degree centigrade) . But biogas can be produced in around 2-3 days during activation period (initial period of bio-digester activation). I installed the system, put fresh cow manure in the system and start to get gas in 2-3 days.
I've used phyre2 to model the protein for the simulation using Gromacs, but later I found that 2/3 proportion of the structure ( except for IDR ) had been already determined by X-ray crystallography.
The known structure contains Zn2+ to stabilize the structure of the entire protein, so I doubt phyre2 can predict decent structure. How should I model the structure of proteins using a known structure as a part of it?
Whether Acoustic Vibrations can be used to damage / fracture the CORONA VIRUS (COVID-19) structure ?
Acoustics with resonance frequency of the COVID-19 can be used to prevent this Pandemic ?
Hi, I am wondering that if variable X is directly proportional to Y and when graphed together have a significant linear correlation of R2=0.97-0.99.
Where, let's say:
Where a, c are constants. Although b(t) is not a constant the relationship between X and Y remains linear.
If Z= ln(X2/X1)/t2-t1
Would it be correct to assume ln(Y2/Y1)/t2-t1can be used to get values for Z as well?
Are there any papers that follow this sort of idea in engineering/mathematics and have been used in science such as bioprocess engineering?
Thank you very much for spending time reading this.
We are looking for a biocompatible immiscible liquid (with water) with low viscosity & surface tension to enable flow in a microchannel system. Most such liquid tend to have some limited mixing at interface with water based solutions at varying temperature and pressure conditions. We are looking for something that provides least mixing at such varying conditions.
Thanks in Advance.
What do you think about the balance between exploring widely different designs vs. local optimization at different levels of biology (genomics, transcriptomics, proteomics, anatomy, etc.)? Which levels are more or less modular or plastic?
In the endocrine system, for example, one feels that having tropic hormones (i.e., those controlling the release of other signaling hormones at other glands) may offer a finer and perhaps more robust regulation, compared to a being where all hormones were non-tropic. However, the anatomic location of elements in these networks is not trivial. For example, in the renin-angiotensin-aldosterone system, renin is produced in the kidney, and aldosterone eventually exerts its effects in the kidney as well. However, the intermediate step by angiotensin-converting enzyme (ACE) mainly occurs in the lungs, which could introduce a delay in the regulation.
Do we have good explanations for the sites of production and action of different hormones in the body? Are there common principles to be learned as optimized by evolution in this respect? Or are happenstances/contingent evolution stronger determinants?
Thank you for sharing your thoughts!
I am looking for the efficient protocol to transform D. hansenii either chemical transformation or electroporation. I have already tried a protocol described by Minhas et al. 2009 (10.21769/BioProtoc.3352), but it did not work. It will be really appreciated if any suggestion or opinion will come.
Thanks in advance.
Looking forward for the discussion.
I would like to know what are the protocols, assays and theory to study a nanomaterial interaction with cancer stem cells (and not normal cancer cells).
I am not a graphic designer!
But now, I am looking for a Windows-based software to help me in the easy drawing of sophisticated 3D illustrations in the field of biomedical engineering and biomedicine.
* The most important criteria for me are to be user-friendly, as well as to be easy to learn and work.
* Moreover, that would be great if systematic tutorial videos of this specific software will be available on the web (Lynda, Udemy, etc.) to learn how to design complex structures (DNA, blood vessels, etc.) and organs (lungs, heart, etc.).
I do appreciate your useful comments and wonderful suggestions in advance! especially those who have previous experiences in illustration of images in the field of Bioengineering.
What are different coil shapes used in Transcranial Magnetic Stimulation? What are their differences (in induced current)? Do they have different applications?
Several G- bacteria including Salmonella Typhimurium and E coli, have been engineered to treat cancers. One of the main things been found is that those bacteria can specifically colonize tumor tissues at a ratio as high as10000:1 over health organs. Bacteria also proliferate selectively throughout tumor tissue. What makes those bacteria specifically target tumor cells?
Hello. I am currently working on a project which involves silk fibroin. The problem is , I'm facing gelation in different steps almost each time. I made 4 attempts till today. At the first attempt I was able to obtain %8 silk fibroin but on other 3 attempts I faced gelation problems twice at dialysis stage and once at autoclave. I'm sharing the protocol which I use. I hope you can give me some advice on the issue.
1. Prepare 5 grams of cocoon shell on a petri dish. (Cut them into small pieces)
2. Add 4.24 mg NaCO3 in 2 liters distilled water, dissolve the NaCO3 and heat the solution to 90 celcius degrees.
3. Add the cocoons to the hot distilled water and wait 30 minutes while stirring.
4. Take out the silk and put into a new beaker inside 2 liters of distilled water (at room temperature) for washing.
5. Wash at least 3 times for 20 minutes. (I'm washing it 4 to 6 times and I rinse the silk with distilled water with squeezing it between water changes) (I make sure that the soapy feeling is lost)
6. Put the silk into a petri dish.
7. Let the silk dry at 37 C for 24/36 hours.
8. Take the silk out and weigh it. Cut the dry silk into pieces.
9. Prepare lithium bromide (9.3M) solution and put 4x of the silk's weight:
-The amount of LiBr: [(86.65x9.3)/1000]x4x(Silk's weight)
-The amount of distilled water: 4x(Silk's weight)
10. Dissolve LiBr in distilled water with magnetic stirrer and add it onto the silk in a 50mL beaker.
11. Put the solution into 60 C incubator for 4 hours. (I waited 4 hours for first 3 attempts, then I waited 6 hours at my final attempt which solidified while autoclaving)
12. Put the liquid solution into dialysis sacks ( Sigma-Aldrich D6191-25EA; 12,000 Da MWCO) and tie the sack from up and down. Leave a little space on top.
13. Hang the dialysis sack in 2 liters of distilled water and open the stirrer.
14. Change the water in regular intervals (after 1h, 3h, 6h, 10, 20h) and rinse the LiBr for 2 days.
15. Open the sack and put silk fibroin into a glass bottle.
17. Take 1 mL of autoclaved silk fibroin and put into a centrifuge tube without closing the cap. (Note the weight of centrifuge tube and the silk fibroin)
18. Put the tube into the incubator (60 C degrees) overnight.
19. Weigh the tube. Calculate the amount of dry matter and then calculate the percentage of silk fibroin.
-The lab's temperature is around 26 C degrees.
- I use different stirrers to avoid heating the water at step 5.
-The liquid I obtain after LiBr application is very viscous compared to the videos I watched.
-The cocoons are present at the lab for 4 years and they are being kept in room temperature in a drawer inside a plastic bag.
I want know what type of PPE and mask has been used by various region and why that particular Region using these particular PPE. And in future what type of PPE they are expecting to use. For example in Bangladesh we are seeking a PPE that can be reuse upto 10 time Against COVID-19.
THANKS in advance for your cordial answer.
I want to detect relative movement between fingers with wearable sensors. Which could be an appropriate technology for this?
As we know that some researcher are suggesting the diffusion of corona virus by nanomaterials. it may be that corona virus can be deactivated by spinel [AB2O4] nanoparticles. i want know some points, which is given below-
- What will happen, if we put corona virus in the magnetic field created by the nanoparticles?
- Corona Virus size is similar to nanoscale particles size, so, Is there any probability to change the structure of corona virus by attaching spinel nanomaterials ?
- can spinel nanoparticles stop the reproduction of corona virus?
I want to design a circuit that can record mice brain's signals.
The signal to be recorded includes the local field potential and the action potential of rat brain nerve cells.
I am a bio-engineer student looking to do an internship with a biotechnology company but i am out of options and less experienced on how I should go about it. I will to be put in the right direction and path which will favor my career.
I am planning on performing expansion microscopy on relatively thick (300 um) tissue slices and doing a post-expansion stain with some nanoparticles. It seems that the expansion process should make the tissue more permeable, but I am having trouble finding a direct description in the literature which supports or goes against this intuition. Does anyone know whether or not a post-expanded tissue has greater permeability than an untreated fixed tissue sample? Note: my nanoparticles are rod shaped and have dimensions of 27x60 nm, but I could also try spherical 5 nm nanoparticles if needed.
We always remember the most important research in our life since we find it was the critical step toward successful academic and scientific career. For me it was the first paper published in 1991 while I was a student in PhD program. Actually, it was published in a very important international journal as shown below. I do not know if you remember that important paper you published?
Zakaria Al-Hassan (Al-Qodah), Viara Ivanova, Elena Dobreva, Ivan Penchev ., Non-porous magnetic supports for cell immobilization, Journal of fermentation and bioengineering 71 (2), 114-117, 1991
I want an initial direction, like how can I use IMU sensors on knees (e.g upper leg and lower leg ) to estimate body state e.g running, walking, climbing, etc. The basic idea I have is to use 2 IMU sensors on knee position (upper and lower leg) , and to get data or make a data set of it. Then process it using deep learning e.g CNN or ANN etc. But point is..are there data sets available on which I can test CNN etc to see either it works or not. Need guidance about data sets, from where could I get IMU knee based data sets, so that I can focus on my algorithm only.
I was wondering if commonly used tagging systems (like FLAG, Streptag or His) can be used as a docking site for knocking down -or even knocking out- tagged proteins
The goal would be to add a third-party molecule/fusion protein that, upon recognition of the tag, could deplete the tagged protein.
Thus, we could have a potential conditionnal KO model by repurposing old/unused transgenic model/cell lines.
I read some stuff about targeted proteolysis and degron systems for example, but i haven't find anything that could be used with common tags.
I'm thinking about using it in mamal primary cell culture or human transformed celle lines.
Does anyone have an idea about it ?
Can someone give me a reference where I can find the definition of log reduction of bacteria (due to UV-C irradiation) in terms of disinfection? In other words, how much log reduction is required for disinfection?
I was trying to model my Dean flow fractionation system by using Comsol. As it is known there are 2 main effects at such a system for particle separation: Dean vortices and net inertial forces. I modelled my microchannel and first I solved it for only fluid flow, then I added my particles by using particle trajectories module. However, I realized that Comsol does not really takes inertial effects into account. For this reason, separation never occurs in such a model. To solve this problem, I think it is necessary to introduce the inertial effects as an ODE. At this point I have some problems:
1) How I can model a force which changes throughout the geometry? I think it is necessary to define a coordinate system in the microchannel to reference distribution of intertial forces throughout the microchannel crossection. There are so little tutorial about this topic at Comsol's website.
2) We still do not know the exact formula of the net inertial forces. In such a situation how one can write a ODE to model this forces?
I have asked a similar question to the Comsol Forum several months ago, but I could not get any answer. I appreciate any kind of help (any tutorial, example, comsol file etc.).
Thank you in advance.
I'm using abaqus dynamic implicit solver to analyze hip joint. There is not problem like this with static step. but i have to use time depended loads. so when i use dynamic implicit; there are stresses only in force applied area but no stress or displacement in anywhere else. i also tried with dynamic explicit step and concentrated and pressure loads but the problem didnt solve.
i've used frictionless surface to surface contact and fixed a region far from load applied area. and material properties defined by mimics software.
i'll also add a photo and abaqus files fore details. thanks alot for your help.
As the Guest Editor of the special issue of the flagship publication of IEEE Computer Society, IEEE Computer, on "The Next Wave of Machine Learning Applications," I invite submissions of path-breaking uses of ML. Details and CFP below.
Keywords: Deep Learning, Artificial Intelligence, Big Data, Biotech, Quantum Computing, Social Innovation
Please also let me know if you wish to serve as a reviewer for this special issue.
I'm setting up a new temperature system using PTC-10 with RG-01, however, I couldn't find any useful information on the internet about it. I would like to find out how to extract temperature information from sensor and how to control the system from a software (ex. using NIS elements).
Autopsies show that there are two abnormal structures in the brain with AD called plaques and tangles. Plaques are made from a protein known as Beta-amyloid and tangles are made of Tau protein. These proteins also exist in a healthy brain. What make them to lose their normal function and cause the cell death?
Some of you must have read about the new FDA approved genetically engineered salmon (they call it bioengineered salmon). As they mentioned, it can grow twice faster than the natural ones and it reach the commercial size in 18 instead of 36 months. I will not start the debate about if it is safe for human to consume it or not specially on the long run (of course many will say it is approved by FDA), I would rather prefer to discuss its impact on the environment. You may know that the salmon fish consumes 5 kg of fish/1kg to produce 1 kg and it takes 36 month to reach the commercial weight. With the new bioengineered salmon, it means that the double amount of fish will be consumed in half of the normal production cycle (18 months). Wouldn't that harm the environment and contribute more to the depletion of the marine resources of other species? Some will say it will be grown in special fish farms...but what if some of the eggs of such bioengineered salmon were leaked by somehow to the sea, what is the impact of such leakage on the natural balance of marine environment??????????????????
I've been researching strategies for the humane genetic engineering of a freshwater seahorse for some time now. I've found some research into developing an artificially convergently evolved specimen based on freshwater pipefish with scoliosis, however, it seems this is a dead project. These animals will likely suffer severe health issues and other unknown consequences.
I'm now curious if it's possible to approach this from the opposite direction. The H. kuda is a euryhaline organism, capable of surviving in dilute seawater ~15 ppt [G. V. Hilomen‐Garcia 2003]. This same study showed it was feasible to push this down to even 10ppt and 5ppt. Is it possible to selectively breed specimens which show adaptations to the lower salinity (most likely represented by a lower increase in total body water) while simultaneously decreasing the salinity to < 2.5%?
If possible, has this been attempted in a controlled environment? It seems vastly more feasible to prepare a saltwater species for a more dilute environment than vice versa.
Moon Jellies have adapted to survive in completely freshwater lakes (which is counterintuitive when their organs rely so strongly on a proper salinity).
In addition, I'm curious the biological affects of different salts on saltwater fish: particularly between Marine Salt vs Aquarium Salt. It would be interesting to know if a euryhaline would function the same in a saltwater environment comprised of Aquarium Salt, rather than Marine Salt. If they do, then a very slightly brackish salinity (which several freshwater fish can thrive in) could bridge the gap between a hybrid estuary environment and freshwater. (I recognize these are vastly different and Marine Salt has other components besides NaCl.)
This thought came from calculating the salinity (ppt) of slightly more than the recommend 1tbsp Aquarium Salt per 5gal -- which comes out to about 1ppt (1.25tbsp per 5gal).
I need to learn cmc value of popc but ı did not any informantion about cmc of popc if any one who know value of popc , ı will request your helps.
in order to prove the veracity of an microrganism aggregation, I need to know if some substances around the cells are in fact polymeric extra-cellular substances (EPS), so id like some kind of methodological where I can easily prove, the ideal is something like a substance that reacts to SPE and visually can be proved.
There are different ways to produce IgG.
One is using intact IgG from blood/plasma donors. The other one is using genetically engineered/recombinant methods which use yeast cell as host.
Which one is better? What are the ads/disads? Which one is more common? and why?
I would appreciate it if you could refer me to some helpful papers.
I have a fluid mechanics questions. Suppose that we a submillimeter needle that is already inserted in the soft tissue. water can run through this needle if the pump is on. suppose we turn the pump on. Waterjet with initial velocity of v0 starts to cut a channel in soft tissue. There is also backflow as this cuts the tissue (backflow from the same cut channel). my question is what happen to the velocity of the waterjet when it hits tissue and goes through backflow. Is it time dependent? Is it depth dependent? Experiments showed that it is velocity dependent since the depth of cut reached from 0 to say 4 mm in 30 second and after that it increases with the order of like 0.01 mm. So it got me thinking that velocity is depth and time dependent. My pump provided volumetric flow rate Q ml/min so the average velocity of waterjet at the nozzle is v = Q/A.
I have developed a mechanics based model for waterjet based on tissue properties and waterjet needle properties including velocity of waterjet. However for Q = 50 ml/min and 0.32 mm needle the velocity is approx. 10 m/s. including this velocity in model predicts a depth of cut in the order of meter which it should be in the order of mm. How can I include the effect of depth and time in my velocity. Any help is greatly appreciated.
Please also see the attached cartoon for a simple demonstration.
Thank you for all your support.
Thank you chandra mohan , Christian Janiesch and Ramin Sedaghat.
Looking for more published projects where students can get benefited by referring these documents.
Please share the docs directly into firstname.lastname@example.org or reply me here.
- Relationship between crude protein content and keratin
- Any existing mathematical expression or equation relating these two?
- Hair keratin extraction
- Crude protein
Can I fabricate same height of main channel(h=5um) and narrow channels(5um) which is joint between two main channel(5um) through multlayer lithography?.kindly give valuable suggestions.thanks
I have studied a series of articles which are related to increasing memory or focus.
I am going to design a mixed drug containing various supplements including DHA (Docosahexaenoic acid), Citicoline , Acetyl-l-Carnitine (ALC) , Phosphatidylserine (PS) , Vinpocetine , L-alpha-glycerylphosphorylcholine , vitamins, minerals, nanomaterials, traditional nutrients like Bacopa Monnieri , Huperzine A , Ginkgo Biloba , so on.
I need to know, is it possible to mix all (or some) of them or not?
My main aim in this study is to increase memory by different methods such as:
Increasing Blood Brain Barrier permeability to nutrients.
Increasing cell respiration of brain mitochondria.
Improving nerve cells which are damaged in Nerve-racking events.
Improving Gut Microbiota balance connecting to the gut-brain axis.
Increasing blood pressure to brain.
I am looking for a motivated supervisor and researcher to start this project. Necessarily, I need your valuable comments about the project.
Is it a novel plan?
Is there any obstacle on the way of my proposal?
Can I start the animal trial on mice or rat?
Would be the drug useful for Alzheimer patients or other mental disorders?
Any helpful comments will be appreciated.
With Kind regards,
In telecommunications, a transmission system is a system which transmits a signal from one place to another. The signal can be an electrical, optical or radio signal.
Can we consider some of the human body systems as transmission systems and then model it using telecommunications' concepts for better understanding?
If we do, can someone please provides some examples of these systems and determines their basic elements(message, transmitter, medium and receiver)?
To get an idea about the resistance change due to deflection and debonding effects (of the gauge from the substrate) due to low adhesion, I need some analytical models.
Generally speaking, for the deflection-resistance part there are some geometrical models that consider classical beam theory and debonding can be modeled with Coherent Zone Model (I think).
I would appreciate, if you could give your opinion and suggest more concrete models for those objectives. Also which software (COMSOL, Ansys..) would be the best to simulate such structures?
I build a chamber (10ul) in a PDMS for PCR application but when I heat the chamber (after 5-6 cycles), evaporation occurred and vanish the liquid in the chamber.
It seems the inlet and outlet of the chamber have leakage.
Is it possible that the vapor leak from above of the chamber (2mm PDMS)?
- i am very interested in your lung bioengineering project. have you performed any animal lung transplants using a bioengineered lung?
- would you direct me to the 2 pilot studies that you mentioned in the description of the lung bioengineering project?
Ihab Abdelfattah, MD
Cardithoracic surgery dept, cairo university hospitals
hi everybody .. I just want to know what is the best way to extract protoplast from wheat plant and wil be so thankful if you provide me a previous certified article or study.