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Bioaccumulation - Science topic
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Questions related to Bioaccumulation
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by NaCl phase separation, final filtration or centrifugation, and counting them under the microscope?"
I have noticed that the micro and macro algae being studied to bioaccumulate Fukushima radiation aren't reported to uptake the Cesium and that Pacific plankton are increasingly reported as bioaccumulating radiation. I think there is a connection between Cesium, plankton and the extra oceanic carbonate (from higher CO2 levels). I wonder if any plankton has been tested for which types of radiation are present, since Strontium-90 mimics calcium and the Cesium-134 mimics potassium. Could there be shifts in the oceanic chemical equilibrium equations to favor radioactive plankton? Do diatoms outcompete other phytoplankton in the presence of extra potassium?
Actually I'm researching on factors influencing T-Hg bioaccumulation dynamics in neotropical fishes (axial muscle) from a main river basin in Colombia. A colleague and me have done an additive machine learning approach to describe more accurately this phenomenon. So we're needing researchers with knowledge in mercury pollution in this biota and (if it's posible) with GIS habilities to create appropiate maps. I must say this research is independent so we're not getting payed nor reciving any economic wealth. ¡Many thanks for your interest and time!
Hello everyone,
I want to calculate a ratio, but for some measurements, I have <LOD in the denominator
There is actually a range of possible numbers <LOD
One solution could be to consider: =LOD, or LOD/2, etc. ?
Are there methodological references which I can confront?
Thank you in advance
Culture media for Bioacclimitization of Cd and Cr by indigenous bacterial strains.
Many passive sampling techniques such as SPMD have been used to replace fish. I am looking for some information on why we should replace fish to measure the toxicants.
I hope I will get good guidelines.
Big thanks
I have come across numerous research papers that estimate bioaccumulation factor of heavy metals in fish muscle originating from its surrounding water or sediment as ratio of heavy metal content between fish and environment. Can anyone please elaborate how may i quantify bioaccumulation of heavy metals in farmed fish originating from the supplied feed that contains heavy metal above permissible limit?
I want to write a book chapter on Emerging Contaminants (ECs) concerning Bioaccumulation and remembering the Aquatic Biota. My objectives will be 1. Categorization of ECs, 2. source, fate, and mechanism, 3. research gaps, 4. effect on an aquatic ecosystem.
I am asking for help, in the Categorization part and effects on the aquatic ecosystem part.
If I want to portray ECs adverse effects how should I do it? random consideration of aquatic biota or describe every trophic level individually with some examples?
I hope I will get help.
Thanks in advance.
In fear of corona disease, the whole World has been used many disinfectants and chemicals. Thus it needs to think about their bioaccumulation effect and suitable remedy measure simultaneously to save the Earth from any type of environmental problem in the near future.
The mycelium biomass is used for heavy metal bioaccumulation in vitro settings, which will be subject to Atomic absorption spectroscopy. In order to get the dry/wet weight of mycelial biomass and to prepare samples for AAS analysis how can I separate and get clean mycelial biomass from PDB?
I'm looking for databases for (eco)toxicology data of chemicals. Specifically; biodegradability, aquatic toxicity, and bioaccumulation. A strong interest is also for data of ionic liquids. Please recommend both public and commercial databases.
I am currently proposing a study on the metal uptake of a particular plant. In the study, I am going to spike my pot experiments in treatments with varying concentration of the metal of interest. One of the parameters I want to find is BCF. I am aware that it is a basically a ratio between the concentration of metal in roots/concentration of metal in the soil. I am confused however with this concentration of soil. Am I supposed to use the concentration of soil after the remediation or the concentration of the soil before the process in solving for the BCF? Thank you and God Bless!
Environment, Environmental pollution, Bioaccumulation or Ocean pollution
Hello, everybody! In your opinion, in an environmental risk assessment, if exists, which is the most critical criterion: bioaccumulation potential, acute aquatic toxicity, or biodegradation? For example: which compound offers more risk to the environment and why?:
Compound 1: very high acute toxicity, low bioaccumulation, high biodegradability;
Compound 2: very high bioaccumulation, low acute toxicity; high biodegradability;
Compound 3: very low biodegradability; low acute toxicity; low bioaccumulation.
Thank you in advance!
Since its emergence in 2019 the SARS-CoV-2 remains a challenge for scientists, clinicians and the overall population to solve. Besides its rapid spread and harsh respiratory distress it did induce, further secondary outocomes are observed. In particular, recent works highlifhted a preferential accumulation of its spike protein in skin tissues, specifically sweet glands. It appears that skin epithelial cells could also be a secondary host-cell of the viron.
this instigates several questions including:
i. did the viron targets all epithelial cells, such as skin and gut epithelia?
ii. if so, does it really rely on angiotensin converting ezyme receptor abundance in these cells? would these cells act as reservor for the virus ARN?
iii. could spike protein modify the skin physico-chemical properties?
etc.
I really wish to have a deep discussion of this fact
Are there any indexes ( like Hazard Index) to calculate the potential health risk associated with consuming food (commercial fish species) which bioaccumulates medical substances like (paracetamol, amoxicillin, venlafaxine)???
In our country, a lot of water sources are found to be contaminated with arsenic (As). But, here, experts said that, using arsenic contaminated water is not harmful for us, we can use it in our daily needs except drinking.
My question is - using arsenic contaminated water for our daily needs, have any risk of bioaccumulation by surrounding biota? Does it have any associated human health risk?
Chromium is one of the main chemical ingredients of tannery industries. Those industries also produce a lot of chromium contaminated solid wastes. what are the biotransformation and bioaccumulation procedures it follows to expose in human body?
Hi, Scholars
I have needed the laboratory reports which covered the following topics
1) Visualize the process of bioaccumulation and biomagnification
2) Distinguish between these concepts using basic calculations
3) Estimate the amount of energy gained /lost through the energy transfers of a typical food chain
4) Review trophic level names and energy characteristics
I'm currently working on a project on heavy metal concentration in contaminanted soil in a mining site and and the toxicological processes governing heavy metal uptake from soils into plant tissue system. So far I have used some indices such as BCF and BAF in my evaluation. But I'm hoping I could do with more indices to validate my results.
Your inputs will be highly appreciated
Microalgae break down heavy metal through the mechanisms of bioaccumulation and biosorption. What mechanisms do microalgae use in breaking down petroleum hydrocarbon?
Bivalve specimens are preserved in 70% ethanol for about 6 months. What is possibility of being able to extract DNA/RNA of bacteria that was previously consumed?
I am currently workin on the evaluation regarding the bioaccumulation potential of the chemical substance bis (4-chlorophenyl) sulphone (substance evaluation under the REACH regulation).
My specfic question now is:
Are there any recent publications (any research) available who determined this substance especially in wildlife birds? It would be very helpful if there are monitoring data available?
The reason for that is that there concerns about this substance regarding its bioaccumulation potential espacially in bird species.
Thank you in advance!
Per- and polyfluoroalkyl substances (PFASs)are a ubiquitous group of environmental chemicals that cause numerous detrimental impacts on the environment and human health. According to performed studies, these metabolically inert chemicals enter the body of humans and animals and transfer into target tissues such as liver, kidney, bone, skin, etc. with the help of protein carriers in the bloodstream. From a dermatological point of view, what toxic consequences are most likely to occur in response to the dermal bioaccumulation of environmental contaminants such as PFASs? What are the differences between possible toxic effects caused by internal dermal exposure (dermal bioaccumulation) to a chemical and external dermal exposure (dermal absorption) to the same chemical?
Thanks in advance.
Bivalve specimens are preserved in 70% ethanol for about 6 months. What is possibility of being able to extract DNA/RNA of bacteria that was previously consumed?
I am working on pollution studies in India using sea urchin as the major candidate..could you tell me how important it is to also consider the sex-based difference in bioaccumulation of pollutants in this organism..is it mandatory unless there is plenty of samples here.
Bioaccumulation factor is calculated by dividing the amount of a specific metal in tissue with the amount of metal in water. I would like someone to explain to me how to interpret the values. Is it so, that BAF is inversely related to exposure concentrations – they are not an intrinsic property for metals, higher values mean less toxic metal? (read from random literature, ppt/pdfs). What is the basis of any interpretation related to BAF?
Conclusions: NTP concludes that Fluoride is presumed to be a cognitive neurodevelopmental hazard to humans. This conclusion is based on a consistent pattern of findings in human studies across several different populations showing that higher Fluoride exposure is associated with decreased IQ or other cognitive impairments in children.
Please will you share this dramatic news in your networks?
The draft NTP report, written and reviewed by a team of 51 scientists is here:
I have added Cadmium in the soil as three different concentration ranges (10, 50, 100 mg/kg) for my pot experiments. Can anyone suggest me the calculation for adding the chelating agents like EDTA, Citric Acid and Sodium sulphate to the contaminated soils?
Your help in this regards is highly appreciated.
Thank you.
Kind regards,
Anand.
Heavy metals accumlation in marine iveretbrates
Most of pesticides are organic and not water soluble. If it get absorb, there is a lot of chemical changes too. In such circumstances how it get accoumolated and contaminate food cycle? It is true or it is just a phobia created by researchers?
I find that I need a short self-tutored course in phytoremediation and wondered if you could suggest some books. I see that there is a professional society---any thought on joining? My main interest is in the irrigation of crops, especially those consumed raw, with recycled wastewater and thus which crops might bioaccumulate xenobiotics and toxins and how one might look at various plant species as accumulators.
Thanks
Edo McGowan
We measure heavy metals in each and every thing and compared it with standard Guidelines. If the total concentration in water, soil or crop is high, straight away we declared that as hazardous and recommend treatment. For example Chromium is present in the environment in several forms. Chromium III (Cr III) and Chromium VI (Cr VI) are common forms. Cr III is comparatvely safe, while Cr VI is hazardous. We discuss this irrespectively of it's oxidation state. The same is the case with other heavy metals also. If look into the present status of research, every thing is hazardous.
I am carrying out a research on developing toxicity ranking of heavy metals and the work involves evaluating bioaccumulation factor and determining Omics endpoints of the heavy metals using
microbs.
I'm working on the selenite removal from the waste water. In this case I'm using a fungus, which is synthesising the selenium nanoparticle by reducing the selenite from the waste water. The colour (pinkish/red) change in the fungal pellet signifying that the selenite reduction is occurring inside the fungus or on the surface, and it is forming the selenium nanoparticle over their. And, the problem is recovery of these nanoparticle.
Does anyone knows that, how can I recover the nanoparticle from these fungus without applying any mechanical operation (Sonication) means without breaking the whole fungal cell?
What are the possible methods to recover these nanoparticles? Any suggestions ?

A few months ago I saw an article which described a game to play with students to illustrate bioaccumulation in the aquatic food chain using pennies. Could someone please provide me with the reference?
Thanks,
Jena
On 8 March 2018, the Chief Executive Officer of the Australian National Health and Medical Research Council sent an "open letter" to selected local government Councillors who are being pressured to add Fluoride industrial waste to the water supplies of their ratepayers. In the letter it was stated:
"Some people ask if there is a cumulative effect of consuming fluoride over a lifetime. The toxicologists on our expert committee advised that fluoride is excreted regularly by the kidneys to achieve a ‘steady state’ that is safe for humans. This is different to lead which accumulates in the body."
That statement is demonstrably completely false as Fluoride accumulates in many human tissues, including bone, throughout life and never reaches a "steady state".
Are there any scientists who think action is required to correct this act of misinformation?
What are the differences between bioaccumulation and biosorption ?
Crappie and sunfish were caught in the pond. We are trying to decide if we should add percent lipids to the tissue analyses. We are looking at potential human and wildlife risks associated with fish consumption and may need to back-calculate to a sediment target concentration. This is for a series of small ponds at a state superfund site that may have received runoff from a wood treater.
Which one is more suitable to measure heavy metal uptake by aquatic plants.
Can anybody provide me these 2 equations with references?
Regards
Hello,
I'm working on the bioaccumulation of Organochlorines in saweeds and want to know about the analysis method.
Plz help meeeee
It is related to the research on
bioaccumulation of plastics.
Are there any established methods or techniques that have been used to test the concentration of polycyclic aromatic hydrocarbons (PAH) in the mushroom fruiting bodies of fungi? I am interested in a methods for extracting and cleaning a sample of raw mushroom tissue to assess PAH bioaccumulation.
If a soil sample has a number of pollutants which are identifiable and non detectable, is it possible to determine the bioaccumulation factor in an organism surviving in that particular soil?
The correlation between fish (freshwater) bioaccumulation to damage fish organs (gills, kidney, liver, mussels) it seen biopsy? if possible means why it occurred what is function happened in biological activity... can explained in detail to discussion of biological actions.
I need to know the protocol of the technique used in the mesure of the bioaccumulation of chemical fertilizers in the tissues of earthworms
My research is the nanoparticles bioaccumulation dynamics in freshwater food chain. Yet, most dynamic modes were focus on water exposure. I want any body give me some references for food chain.
Stedman had a design that took the solids off ahead of digestion and converted the removed solids to a fuel source. I liked this process as it pulled out a lot of material, which if it had gone through bacterial digestion, would have been converted into solution which is more difficult to control. Is anyone working along these lines? I ask because a lot of the pharmaceuticals are broken into more toxic daughter products either by bacterial digestion or later oxidative processes, and many are mitochondrial toxins (MT). Thus using the effluent as an irrigation source or drinking water augmentation sees these MTs bioaccumulating in irrigated crops or being chronically delivered with drinking water. Thanks------------Edo
I have a data matrix consisting on the water salinity (WS), ionic composition (IC, mg/L) and electric conductivity (EC, µS/cm) for the last 10 years for 2 main rivers in Central Asia (with one reference site and one impacted site at each river). I want to explore the relationship between WS, IC and EC in order to formulate water quality standards on salinization. Does anyone have any suggestions on well suited methods of statistical analyses for doing this? Thanks in advance!
I am interested in study of Bioaccumulation, Bioconcentration and Biomagnification of chemicals in fish through laboratory base experiments. Although I found some protocols/procedures which are exhaustive and lots of variation are there. I am looking for any standard protocol/flow chart procedure for this purpose especially for Biomagnification.
Does phenyl salicylate have the potential to bioaccumulate in animal tissues?
Specifically looking at mechanistic pathways of uptake and examinations of transport across epithelia?
What are the possible chemical/physical techniques that can be used to substantiate bioaccumulation if removal appears?
Exploring to see if such a test exists that would work in the field that do not require collection/storage/expensive lab tests. Wondering if multiplex colormetric assays exist for wildlife, particularly for birds of prey using blood, tracheal or cloacal samples, feces, etc...
so far I don't think it exists. Thanks.
The model is provided in the attached papers.
Dear friends, Hello, I have enjoyed the conversations very much and first of all I want to thank you for sharing. I am a PhD student in marine biology and decided to work in the subject of Microplastic ingestion by fishes in Southern Caspian Sea. At first part of my work, we are going to examine fish intestines to find any anthropocentric object and in second part, feed juvenile fish with mixtures of food and 0.5 to 5 micron Microplastic (MP) to find their possible cellular movements and effects on cell mechanisms of entrocytes. To find out, I want to use Transmission Electron Microscopy Technique to see weather MP is found in entrocytes of intestine or not and is there any effect on ultrastructure of entrocytes. I have written a proposal and presented it but some experts had doubt about efficiency and practical possibility of this method. I have used the Technique for normal tissues of different fish species but some people think in the EM images, MP will show up as empty spaces and they may be confused with other objects or artifacts. Therefore, I appreciate if you help me in this case. I need to know what you think about it. Happy 2016 and hope you have a nice holiday. Cheers, Zahra
I'm looking for data concerning the levels of trace elements in blood and hair of alpaca, for a bioaccumulation study, but the bibliography on the subject is scarce...
any suggestion is welcome
many thanks in advance
stefania
I am investigating the use of chelates in treating aquaponic iron deficiency.
1. Invasive species, clams are not an issue to sacrifice 2. Only found cyanide extractions for solids and water 3. Testing the runoff--cyanide levels--upstream, downstream and at site of dumping for a coal company 4. Also looks to test cyanide levels in plants, and sediment if you have access to additional protocols
Does anyone know of reliable label-free techniques for quantifying graphene and/or graphene oxide inside cells and tissues of organisms such as algae, worm, etc.?
The Tessier method has five basic fractions, can be added other?
I am wondering if anyone knows of research evaluating the use of earthworms to bioaccumulate and remove anthropogenic chemical contaminants from biosolids destined for land application? I am looking specifically at triclocarban, triclosan, and methyl triclosan bioaccumulation through vermicomposting biosolids. I have come across studies using earthworms as bioindicators of the presence of contaminants in soils previously treated with biosolids but nothing that looks at how efficient worms are at removing the contaminants from biosolids.
Once triclosan enters into the environment, it persists and has been found to be harmful for several species of fish and algae. I want to know what is its bioaccumulation factor in species of fish and algae.
Samples in dried (dead) padina and gracilaria
Light REEs (Yb)..Heavy REEs (La)
It seems that some fish species targeted by both commercial fishermen and amateurs feed on Caulerpa taxifolia. This algae contains nine different toxins including the alkaloid Caulerpina. These poisonous substances accumulate in the meat of the species that feed on Caulerpa taxifolia?
Great symbiotic association was found by investigators between rhizosphere of mangroves and microbial community in past few decades. But do the microbial community help in the process of bioaccumulation of certain heavy metals by mangroves from ambient water and sediment?
For different organisms, the permissible ranges of heavy metals are different. I need the ranges of the mentioned heavy metals as I am working on mangroves and associates.
We found high concentrations of As in P.lividus (maximum 47 mg / kg DW). We have no data concerning As levels in algae or P.oceanica in the same zone but we have As concentration in sediments (maximum similar to P.lividus maximum).
I had read some articles about pendimethalin, those are studied on water bodies only,
What kind of invertebrate would you suggest for monitoring (sporadic) PCB micro-contamination in a small stream (from waste dump soil leaching)? The stream bed is of fine gravel and sand, fishes are not available... Maybe freshwater mussels, warms, larvae of arthropods?
E.g. the uptake of trace elements in transplantated aquatic bryophytes (moss bags) occurs with passive more than active mechanisms in the short term (days-weeks), elevated ratios moss/water (10^3 - 10^5) and significative correlation with exposure time and total/dissolved concentration in the water. Saturation generally occurs after serveral (>4) weeks. Are DGT or other tools more performing with similar costs (transplantation, recovery, sample mineralization and chemical analysis by ICP)?
And can the test be used as a valid measure to determine treatment response effectiveness?
Recent presentation of a family requesting alternative treatment for suspected Chloracne. Conventional medical treatment was sought for over 5years yielding mild relief in symptoms. Cases are 3 adult males and 1 adult female, each with a 10year+ history of suspected chloracne.
I read the word and do not have any information regarding this.
In my current work I have found a decrease of some trace elements (with the exception of Al) in E. prunastri during the spring season, it is possible that within three months of exposure there has been a similar condition?
Give me your opinions.
It is often seen that while calculating concentration factors for biological uptake some researchers calculate dry weight concentration against the water/air concentration, which actually provides a much higher concentration.
Has this been noticed by many?
What fish species are more exposed to phthalate toxicity, pelagic or benthic? Please discuss in the light of the variety of phthalates present in the aquatic environment.