Questions related to Behavioral Testing
when i put the animals in the hot plate with a temp. of 55c animals didnoh pear the temp and give rabid respons is that normal or i have to decrease the temp.?
When I use hot plate device the center of the triangular glass box is higher than that at the edges and when rats were put in the center in give a rabid response and when placed in the edges it give very slow response what is the best way to over com this problem ?
And can I put the animal directly on the hot plate without the base of the glass box ?
And when the rat try to climb the wall of the box it was measured as positive response or not ?
We are currently using the accelerating rotarod test to assess vestibulomotor functioning following experimental brain injury in rats. In this regard, we have encountered that it can be very difficult to motivate animals to run on the rotarod: Some animals jump off the rod after a few seconds, whereas others seem to develop a strategy where they deliberately ‘fall’ off the rod in a more or less convincing manner after a relatively short time interval (e.g. 30 seconds). In both cases the performance/score does not appear to reflect the true vestibulomotor functioning of animals, and the performance of some animals vary significantly from trial to trial.
In order to establish a baseline performance level we have been giving animals 10 trials (5 days of 2 trials) before injury. Subsequently animals have been tested on the rotarod for 7 days (2 trials per day) post-injury. We are using a commercially available rotarod from PanLab/Harvard Apparatus (fall height 21 cm, rod diameter 60 mm, acceleration 4-40 rpm over 2 minutes).
If anyone has had similar experiences and has any ideas or advice on how to increase motivation in the rotarod test, it will be highly appreciated.
I am doing a baseline + 2 rounds of behavioural testing (NOR + NLR) - so total 3 rounds of testing, all 1 week apart in male SD rats. Is it necessary to habituate them before every test, or is habituating them once prior to baseline testing sufficient?
Also, do rats get bored if they perform too many tests in a short period of time? If so, how do you overcome this?
Is there a free software for virtual reality morris water maze tests for humans? Also, are there open arm or other tests in VR format?
I am aware of the issues and pitfalls of an online behavioral testing - i.e. collecting data in a cognitive experimental (visual search) task throughout the internet. Yet, I decided to do a little test:
I want to assess the same visual search task in a controlled lab setting and using an online platform. I am not planning to do a repeated measures setting.
In the lab setting, around 30 participants will do (based on my previous experiments).
Here's the big question: If I want to validate the online method how many participants do I need online? Shall I go with the same sample size or should I aim for as many respondents as possible?
Thanks in advance!
Anyone working on behavioral neurology or using mouse models for behavioral testing, could you please share the valid detailed protocols regarding below mentioned tests;
1) Grip Strength
2)Using Treadmill to measure endurance and speed
3) Treadmill exercise protocol
4) Rota Rod
I have been searching through web to find step by step protocol to perform in vitro whole blood stimulation with LPS (or any other mitogen) using mouse blood.
I do see a few papers describing this method but I was wondering if I could find a step-by-step protocol. I also see many protocols using human blood but I am not sure if it's okay to just follow those protocol, probably changing the LPS dose and incubation time.
The reason I am planning to do in vitro stimulation rather than in vivo is that I want to measure immune function of mice before I do behavioral testing and does not want to prime mice for stress.
Thank you in advance for your help,
It has been suggested that AD is more common in human female population and disease progression is more aggressive in female 3xTg mouse model. However 3xTg males seem to be used quite often in studies as well.
I'm planning to do some behavioral tests on 3xTg mice, there are reports on hyperactivity in aged females, and that estrus cycling may confound assessment of disease-related behavioral dysfunction. I'm not sure if there are any other special concerns with this model regarding the choice of gender.
Also Jax warned that male 3xTg transgenic mice may not exhibit the phenotypic traits originally described, thus I might have to use females since I’m going to purchase from Jax.
Does anyone have experience with this model? How are the females doing in behavioral tests and what are the concerns?
Can someone please explain why it's so common in the literature using the Barnes Maze to have a 15-minute intertrial interval? Protocols for other methods of measuring spatial memory have significantly lower ITIs but for most of the Barnes literature, 15-minute ITIs are always common.
I'm just wondering if I could run Barnes Maze with mice (40 hole set up) and use a shorter ITI and it still be ok in terms of publishing?
Trying to get zebrafish prepped for microct. We want to have the fish suspended in 25% Lugol Solution while they're still alive in order for the solution to better penetrate. I can't find any protocols on how long to incubate the fish for or at what concentration when fish are still alive. I want to induce minimal stress. Thought about adding a small amount of Tricaine to the solution during the process. The fish will be fixed afterwards in 95% EtOH. Protocols or ideas anyone?
I am looking for software to conduct behavioral experiments. I plan to use auditory stimuli and gather responses via a touch-screen Windows computer. I have some experiance in programming (ANSI C, Java, Pearl, Ruby). I am looking for software that is well-documented (user tutorials, well-explained scripting language, helpful support community) that handles touch screen input with ease. Any suggestions?
I am planning to create a mouse AD infusion model by introcerebroventricularly injecting aggregated Abeta42. I have been unable to find information on the differences between aggregating Abeta42 for 3, 5, or 7 days. What is the minimum number of days during which I can aggregate Abeta so that the Abeta injected mice show AD behavioral characteristics? Thank you for your help.
I am currently researching a book on stalking. It is primarily based on my own experience as a case study. The UK law changed recently making stalking a criminal offence, however it is apparent that not all police authorities recognise stalking as a problem outside of domestic violence and this presents a major problem for mental health workers who are one of the most likely professions to be experience this very distressing and anti social behaviour. I am looking for contemporary literature/empirical data ideally from the UK on non-domestic violence related stalking.
I know that mice are disqualified if they don't interact for at least 30 seconds during the test. I am wondering if there is literature on rats and what is considered too little interaction?
I am interested in doing a subchronic drug paradigm and would like to look at protein levels. How long after the last drug treatment is given should I sacrifice the animals and look at protein levels?
Dear all - Are you aware of any study in which authors have tested the same mice in different behavioral set ups? More specifically, I'm interested in studies in which mice were tested for anxiety-, depression-like behaviors followed by ethanol intake or conditioned-place preference experiments?
Thanks in advance for your input and very best regards
I want to test 2 ( High Crowding * Low Crowding) * 2 (Deception* Not Deception) on emotions of consumers (experimental survey study design). I have four vignettes representing (Crowding and Deception, Crowding Non-Deception, Non-Crowding and Deception, Non-Crowding No Deception) vignettes. If I give one vignette for one respondent rather than all, is it ok...
If I want to test all four from one respondent how should I include all four vignette to test emotions of respondents. What is the way that I should include them to test dependent variable. Appreciate early help
Although the importance of these external and internal factors have been shown in the dropout decision of online learners, external factors are the factors which cannot be controlled by the instructor or the program provider (Packham et al., 2004).
As the comparison between online and conventional teaching methods has attracted the attention of previous research, I wish to explore the difference in experience of online dropout and completer, in terms of motivation, satisfaction and isolation, as an attempt to assist online course administrator reconstruct their online courses, and provide information and directions for further studies.
We are conducting an experiment about the impact of physical exercise (running) on neurogenesis in the adult brain. We use a transgenic mice model and so far, we encountered some problems with the mice because they don't want to run...we have tried to attract them towards the running wheel with some food placed on the running wheel. However, this method didn't work so far. Therefore, I want to ask you whether you have any suggestion about what could we do in this situation.
Many thanks and I look forward to your help :)
SILS, Center for Neuroscience
University of Amsterdam
I am currently experimenting with the elevated plus maze (EPM) and radial arm maze (RAM) to test the functionality of the hippocampus after the injection of neurotoxic chemicals such as kanic acid. I test with the EPM on the first day and start habituating for RAM the next day. I habituate the rats for 3 days then start recording. My professor was concerned that the EPM may have an interference effect on the RAM. I wasn't able to find references on it either. Are there possibilities that the experiences on the EPM prior to the RAM affects the performances of the rats on the RAM?
Specifically, there are two main types of settings for mice: 2mA, 2s and 0.3 mA, 5 s. What is the difference? Which is more sensitive? How Passive Avoidance test results may be different between out bread white mice and C57Bl/J mice?
Hello everyone, I am trying to set up a "nondestructive testing lab" for casting metal. Please suggest any nondestructive testing equipment. If possible, then send me the supplier address.
I have performed some preliminary behaviour experiments and I would like to do some power calculations to determine how many more samples I'll need. I've been trying to use an online calculator which asks for mu1 and mu2 values but I'm not entirely sure what these are. Can anyone provide information on this?
Many thanks in advance,
I would like to know whether environmental enrichment in a cage in the study of chronic toxicity affects the results of behavioral tests. Maybe you know the literature on this topic?
I would like to test the cognitive decline in a mutant mouse line. However, when I have run Morris Water Maze with using visible platform, it shows higher latency compared to the wild-type. It appears that they are not blind, but may have bad vision or other problems that may affect the latency. Are there any other behavioral tests I could use to determine that if they have memory loss?
I am planning to conduct research on competitive traits and its effect on competitive states. I would appreciate if someone could recommend me some instrument to evaluate pessimistic trait and cognitive bias consequences. Thank you in advance
We have been using the Morris Water Maze, but I was wondering if there are any more reliable experiments to test for deficits in ACC function in rats.
There are many tools like Peabody Individual Achievement Test, Wechsler Individual Achievement Test, Woodcock Johnson Psychoeducational Battery to assess the school performance in children. However, none of these tests have normative data on Indian children. Hence, I am looking for a tool useful in a Indian setting.
How do you check intellectual ability in laboratory animals using behavior tests, biochemical markers and the molecular mechanisms behind that (gene, protein levels)?
During the animal training behavior test like Eight arm radial maze, I used to wear gloves during animal training some of my friends suggest that don't use gloves, if you gloves the animal doesn't feel your body temperature and it will take time to get habituate animals and the glove's smell also will affect the behavior.
Please suggest me which is best way to train the animal with or without using gloves
As described in "Behavioral Tests After Intracerebral Hemorrhage in the Rat" (Ya Hua et Al. 2002). I cannot find a performance show of that test anywhere.
When using the test validity, perhaps you may face this question: how can I be sure of my test validity? Yes , there are a number of casual methods to measure test validity like content validity, referee validity, external criteria test validity, discrimination test validity and factor analysis for testing validity. Some researchers use the internal consistency as a measure for test validity. In this way we measure the co relationship between the item response and the total score of its dimension and also the co relationship between this dimension and the total score of the test. As long as the correlation is positive and highly significant it says that it is valid score... What do you think?