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Dear, I have a question: why doesn’t this link work when I search for it on Google?
Enhancement of Breast Cancer Classification Using Bat Feature Selection with Recurrent Deep Learning
Why doesn’t this address the paperwork when I search for it on Google? Enhancement of breast cancer classification using bat feature selection with recurrent deep learning.
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Why, knowing that the page exists?
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how can the environment benefit from bat droppings
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I need any serious information on erythroblastic islets in bats (chiroptera)
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Thank you very much, this can really help us in writing our new article.
Best regards
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I am trying to conduct a systematic review for my dissertation at university. I am struggling to find the advanced search filter to add my desired words to narrow my results. My terms are: ("bat" OR "UK bats") AND ("UK") AND ("policy" OR "legislation") AND ("impact" OR "population change"). Would I just enter these key terms in the regular search bar or is there a specific section?
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If you're using Pubmed, the best way would be to use the search bar and follow the terms by [tiab] for title and abstract, [ti] for title only, [pt] for publication type, and [MeSH] for medical subject heading. You can use these with boolean operators OR, AND, NOT, however you think makes your search as sensitive and specific as you'd like.
For the purpose of a systematic review, you're going to need to make your search strategy more sensitive than specific, and that by using [tiab] instead of [ti], and using the synonyms of the terms as well.
For example: (bat[tiab] OR bats[tiab] OR chiroptera[tiab] OR megachiroptera[tiab] OR microchiroptera[tiab]) AND (UK[tiab] OR "united kingdom"[tiab] OR England[tiab] OR scotland[tiab] OR wales[tiab] OR "northern ireland"[tiab]) AND (policy[tiab] OR legislation[tiab] OR law[tiab] OR laws[tiab] OR regulations[tiab] OR legal[tiab]) AND (population*[tiab] OR impact*[tiab] OR change*[tiab] OR effect*[tiab] OR activity[tiab])
You can add words or remove others to make the strategy better suitable for your research question. However, if you're using a different database than pubmed, there are different codes if you will to different databases. You can find tables that can help you by googling the database (e.g: Embase search strategy)
This course can help you learn more about building a search strategy for your systematic review (module 3):
A few more tips in this page:
Best of luck!
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Hello,
I am trying to stimulate Brown Adipose Tissue cells by exposing to cold.
Previous research had shown that in-vivo, the optimal temperature exposure (of the whole organism) is 16°C for at least 2 hours. During the exposure the BAT cells start to to regulate energy expenditure through non-shivering thermogenesis.
I am trying to do it in-vitro.
Any ideas on an optimal temperature exposure?
Thanks in advance,
Alina.
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Alina Wiener did you manage to carry out your experiment?
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I have to perform hnRT-PCR for detection of rabies virus in different samples from bats. I intend to use the well known WHO protocol for pan-lyssaviruses, but I'm not sure about the quality of the RNA extraction. In the WHO protocol they use human beta actin as housekeeping gene for extraction control, but I don't think it will be suitable as the samples are from bats. Can anybody tell me what should I use to check my RNA extraction?
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HI
you can depend on research to choice the housekeep gene but best is GAPDH
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Hi Folks,
The attachments are 5 bat calls, recorded via Anabat detector, from Umluj, Tabuk Province, Kingdom of Saudi Arabia.
I analyzed them using Anabat Insight's BatClassify Plugin. On analysis, four of them are classified as Pip (precision >95% in all four cases), which means they belong to the genus Pipistrelle. However, only one out of the five recordings is classified as [NSL (96%) (1); Bbar (62%) (2)]. It is not clear as to which genera out of the Noctule, Serotine or Leisler’s they belong to. The fact that Barbastelle is not reported from this part of the globe makes it even more intriguing. Moreover, the plugin pertains to species from the UK only and therefore, I need to confirm from experts. I shall be grateful if someone working on this aspect of ecology can confirm the genera and, if possible, the species from the files attached.
Thanking in anticipation
Siraj
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Hello dear friend
what country and present your species sample
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Hi everyone
I am looking for gape sizes of the following species. I would be grateful if you have data or references that specifically mention these species (I am not tlooking for references to general papers on gape size).
Auriparus flaviceps (Verdin)
Brotogeris versicolorus (White-winged Parakeet)
Onychognathus tristramii (Tristram's grackle)
Psittacara holochlorus (Green Parakeet)
Psittacula krameri (Rose-ringed Parakeet)
Pycnonotus cafer (Red-vented Bulbul)
Pycnonotus jocosus (Red-whiskered Bulbul)
Pycnonotus xanthopygos (White-spectacled Bulbul)
Rousettus aegyptiacus (Egyptian Fruit bat )
Setophaga coronata (Yellow-rumped Warbler)
Sialia currocoides (Mountain Bluebird)
Any help is much appreciated
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Have you checked the public trait database AVONET?
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I want to study the emergence and return of the bats in a cave in relation to the duration of the night and day based on data associated with the moment of the sunset and the sunrise during a day in a place located in northern Madagascar. So, I would like to know the website to visit to obtain free and accurate data for my study.
Many thanks,
Riana
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I realize this is an older thread but I'm running into the same challenge and noticing that many of these apparently authoritative sites return different results. Of the four sites I've checked so far (for sunrise in Grunau Namibia October 1st for example) I have a range from 6:24 am to 6:39 am. That's a big difference! Any other thoughts on authoritative sites would be appreciated.
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Since the creation of Genetic Algorithms a number of people have created all sorts of optimisation algorithm inspired by nature and a variety of living creatures. Many of these algorithms are classified as swarm intelligence in opposition to genetic or evolutionary algorithms.
Over the years, my impression, is that people have lost the sense of why developing bio-inspired optimisation and rapidly diverged towards developing bio-inspired optimisation for the sake of it.
The insane proliferation of these algorithms has been accompanied by an equal proliferation of journal papers. The impression is that, as long as a new living thing is used as paradigm, one can publish an unbounded quantity of journal papers.
My impression is that, if one looks at the working principles of all these algorithms, the truth of the matter is that all these algorithms are fundamentally the same algorithm with some differences in the implementation details.
We now have: ant colonies, bee colonies, cuckoo birds (!!!?!!!), pigeons algorithms, fish schools, krill heads, bat algorithms, firefly algorithm, invasive weeds (scary), whale optimisation, grey wolf optimisation (wonder if by changing the colour of the wolf I can publish a new bunch of papers), etc. Try to randomly enter names of living things and add optimisation in Google.
Now my question is: does it make any sense? apart from allowing many grad students to graduate with some journal publications?
Or is it really the name that you give to an algorithm that does it?
Given the millions of species, each one with their own behaviour and ecosystem, I expect more algorithms and papers in the years to come but again: does it make any sense?
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This question and discussion are very important and interesting simultaneously. While reading the answers, an idea arrived, which can somehow help us to better understand the crux of this matter.
One of my current interests is the theory of computation of biological systems. When we compare it to our standard methodology applied to computation, we end up with a Turing machine, von Neumann architecture, and their variants.
The problem is—which was von Neumann very aware of—that biology is computing in massive-parallel ways. Let us take the John H. Conway’s 'Game of Life' (GoL) cellular automaton that is a perfect example of massive-parallel computations.
We found out that in the GoL we can create AND, OR, and NOT gates from glider guns emitting gliders. So far good. BUT! The concern is why we do not study the true information processing withing massive-parallel environments without defining our, human, computer-based logic, and do not explore and exploit 'emergent logic' and information processing in its natural form?
Details of what is just explained here is present in the paper dealing with robust (error-resilient) massive-parallel emergent structures observed in the generalized r-GoL.
Going back to our question. What I do propose to focus our attention on is to go back to biology and study its minute computations in greater detail and depth. From this point, we can try to work towards an emergent, self-adjusting computational approaches applied to optimization.
I do see a clear parallels in the r-GoL research and search of emergent optimization methodologies. It might be a way to avoid endless numbers of optimization techniques originating in a common ancestor.
Was it a sufficiently clearly put idea? Let me know. 
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I mean compiled data, table in an article, etc.
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Hi again.
For Scandinavian bats there is an interesting source of information about this subject:
BAAGOE, H. J. (1987). The Scandinavian bat fauna: adaptive wing morphology and free flight in the field, pp. 57-74 (in): Fenton, M. B. et al. (eds.), Recent advances in the study of bats. Cambridge University Press. Cambridge / London / New York / New Rochelle / Melbourne / Sydney.
Best regards.
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I am trying to use the Marantz model PMD661 to measure bat activity, but have been unsuccessful at attaining any data using the model, despite being able to see the bats present. I was hoping there was a way to adjust the settings so it could pick up on their calls. Does anyone have any experience with this model that could help?
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Te recomiendo contactes a la benemérita universidad autónoma de puebla, en la facultad de mecatrónica ya que ellos pueden desarrollar la tecnología adecuada para tu necesidad.
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Hi,
does anybody have information that can share about pain recognition in bats?
Citable sources will be especially appreciated.
Thank you
Javier
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Hi Carl
thank you for your suggestion. I am aware of these books, but I don't have access to them, unfortunately.
Javier
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One of my undergrads created a phylogeny based on few human hCov19 and few from other hosts including bat, panguline, and dog. He found two viral genomes one from an Australian and one from a Singaporean clustered with the one isolated from canine. This seems interesting! Any study to support this finding?
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Hi,
I am trying to run a vumat subroutine, but there is some abaqus-fortran linking issue.
I use
abaqus 6146
Micrasoft visual studio 14.0
parallel studio XE 2016
I added these command in my .bat file:
@echo off
call "C:\Program Files (x86)\IntelSWTools\parallel_studio_xe_2016.3.059\compilers_and_libraries_2016\windows\bin\ifortvars.bat" intel64 vs2015
call "C:\Program Files (x86)\IntelSWTools\parallel_studio_xe_2016.3.059\compilers_and_libraries_2016\windows\bin\ipsxe-comp-vars.bat" intel64 vs2015
call "C:\Program Files (x86)\Microsoft Visual Studio 14.0\VC\vcvarsall.bat"
"C:\Program Files\Abaqus\6.14-6\6.14-6\code\bin\abq6146.exe" %*
but I got the error message:
ifort: command line warning #10161: unrecognized source type 'Files\Abaqus\6.14-6\6.14-6\code\include"'; object file assumed
ifort: warning #10145: no action performed for file 'Files\Abaqus\6.14-6\6.14-6\code\include"'
End Compiling Single Precision Abaqus/Explicit User Subroutines
Begin Linking Single Precision Abaqus/Explicit User Subroutines
LINK : fatal error LNK1181: cannot open input file 'oldnames.lib'
Abaqus Error: Problem during linking - Single Precision Abaqus/Explicit User Subroutines.
This error may be due to a mismatch in the Abaqus user subroutine arguments.
These arguments sometimes change from release to release, so user subroutines
used with a previous release of Abaqus may need to be adjusted.
When I checked the system information of abaqus, it showed it is linked with intel fortran compiler 16.0, but when I ran the abaqus vertify, I got the sam error message as above for the subroutine linking.
If you have any idea what could be the problem, please help me, thanks!
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Genetic analysis of the COVID-19 virus shows that it is very similar to a coronavirus in bats but the receptor binding looks like the receptor for SARS. This could happen naturally with slow changes of one in ten thousand sequences each year. This appeared all at once. This gives support to the belief that the Wuhan lab took the bat virus and attached the SARS receptor. So, does the COVID-19 show antigenic shift and drift?

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Antigenic drift refers to the accumulation of genetic mutations that cause an alteration in the surface of the virus. This is one of the main reasons why a novel flu vaccine is required every year. Antigenic shift occurs when segments from the genome of two different viruses combine to make a novel strain. Coronaviruses are not prone to undergo antigenic drift or shift
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Hi everyone. We want to identify viruses in bat samples. The identification is enough for us (we do not need a whole-genome sequence). Most studies use K8N random primers for cDNA synthesis and random PCR amplification. Are K8N primers specific to viruses? I could not find any information about the origin of K8N primers. What would happen if we simply convert them into cDNA and sequence them by using shot-gun?
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From what I understand, K8N are just random primers (referenced here . This reference cites a previous paper (ref #7) here
Bw, Simon
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I work with bat's helminths taxonomy. The ideal is to work on fresh hosts, but some bats species are highly threatened and it's not possible to obtain fresh carcasses or other alternatives. For these cases, I'm thinking about recovering the parasites from bats in biological collections. However the helminths recovering is difficult because of the dehydration and stiffening of the viscera when stored in formaldehyde or alcohol solutions. I've been trying to store some samples in water for some hours or days, but the visceras didn't rehydrate at all. Does anyone have some tips?
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The process of fixation is irreversible - I agree to that.
But I am wondering, why you would like to rehydrate the viscera. Wouldn't it be easier to simply 'wash out' the helminths (I suspect that they are in the lumen of the intestines?), then make a cell block of the fluid and then look an it via microscope?
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We try to isolate viral RNA and DNA from bat oral, rectal, stool samples. We tried Zymo Quick-DNA/RNA MagBead and we got minus concentrations. Then we increased the bead-binding time and decreased the elution volume. Again, we could not get good results. Is there anyone who have used this kit before and can you please give us advice about how to increase our yield? Thanks!
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I never used Zymo Quick-DNA/RNA MagBead Kit. But, I have been using other magnetic bead-based DNA/RNA purification kits both in the manual and automatic extractor.
Manual bead-based purification steps are critical as there are many possibilities to lose or wash out your desired DNA/RNA without an expert hand.
You can follow the troubleshooting guide from the link below;
In essence, to improve your DNA/RNA recovery,
> Check the sample input amount
> Perform Proteinase K treatment to the sample prior to purification
> May increase bead binding time
> Check bead quality (bead can lose binding affinity)
> Remove residual ethanol during the dry step
> Do not over dry beads, it will decrease yields.
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I am not aware of any published key to the ultrasound calls of Western Australian bats. I am trying ton identify recorded calls in the Perth hills.
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I am interested in products of less than 1 g in weight, for bats of about 10 grams. Thank you for your recomendations in advance.
(Im aware that telemetric transimtors of such weight exist, yet am currently looking into other options)
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Hi
I am using the PSO code to find the minimum cost of energy consumed in a building. I wrote the program of strategy by using the if loop but I have a problem with the result so anyone could help me please by giving me an example of strategy program.
equation of power balancing : P_pv (k)+P_wt (k)-P_bat (k)+P_g (k)-P_load(k)=0
Best regards
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Dear Researcher
What parameters do you use and need to be improved, I can help you and cooperate with you in producing scientific research using original PSO optimization algorithms or hybrid algorithms from PSO versions
I wish the best for you with Regards
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Over 24,000 piglets died in farms with swine acute diarrhoea syndrome coronavirus #SADS-CoV transmitted from #horseshoebats in China in 2017.
A study published in Nature in 2018 [1] found SADS-related CoVs with 96–98% sequence identity in anal swabs collected from bats in Guangdong province during 2013–2016, predominantly in horseshoe bats (Rhinolophus spp.) - the known reservoirs of SARS-related CoVs.
Interestingly SADS-CoV only killed the piglet but older pigs survived the disease. It is likely that pigs were naturally immune to the family of coronaviruses, perhaps due to environment, historical exposures, etc. The piglets died but infected pigs sold off to the market (apparently they were not ill) and consumed by the public.
Is it possible that SADS-CoV mutated in the pig and transmitted to humans? Is pig the intermediate host between bat and human for the CoViD-19?
The genetic sequence for the SARS-CoV-1, SADS-CoV and SARS-CoV-2 (the novel coronavirus) is already published - can we compare the genetics and check if there is any evidence to support this hypothesis?
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This question, with a certain complexity, has come back onto the 'official' media and scientific radars. Read for example:
And also (among other items in what will now become a proliferation):
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Hi
I am working on the optimisation of the cost in a hybrid system consists of PV,Wind and battery.
I started my pso code but i have a problem with result please could anyone help me and told me if my function program of my power management strategy is right.
the power balance equation:
P_pv (k)+P_wt (k)-P_bat (k)+P_g (k)-P_l (k)=0
you can find attached the documents.
Best regards
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Dear Abir Hasnaoui
Have a look, these articles might be helpful:
Smart Microgrid Management: A Hybrid Optimisation Approach DOI:
  • 10.21203/rs.3.rs-118351/v1
Particle Swarm Optimization for Micro-Grid Power Management and Load Scheduling DOI:
  • 10.32479/ijeep.8568
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I am writing a short note about bats diversity in two sites (the sampling period for one site is of two days and in the other site is of six days) but besides the species and abundances, I also have information about the sexes of individuals. Any recommendations of papers or about what could I do with the sex information of the species?
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Just include all variables in your analysis and see what it shows. Also, a paper is better than a short note :)
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How many average genetic distance values of mtDNA control region Dloop for indicative conspecific populations or valid species in Chiroptera?. Thank you
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Hi Husni,
as far I could see, there is not a "magic number" for Chiroptera. Only three species have records in genbank for the dloop region. Therefore, it's really tricky to get a safe threshold, if we can say that exist such a thing.
I'm personally not a fan of thresholds, but you could try to explore the intraspecific genetic distance of the sequences on the database. It will give you a clue about how much this sequence is instraspecifically polimorphic as well as it should overlap the between species distances.
I should also recommend you to take a look at the coalesce analysis like GMYC or the PTP (Poisson's Process Tree) and their bayesian implementation.
Good luck
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Hi all.
One of my students and I are currently trying to assess cave bat vulnerability globally. If you have cave bat distribution data that you would be happy to share (and you will of course be fully acknowledged) it would be great to hear from you.
Thanks and Happy New year!
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yes! DM
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Hi people,
I am studying bat brains through endocranial casts and I cannot identify which structure could be present in sort of a canal located dorsally to the cribriform plate. It is like olfactory nerves exit the olfactory bulbs through the punctuated cribriform plate, while another thing exit the olfactory bulbs more dorsally, going over the cribriform plate. It's really bizarre because it's like a plate of space starting at the antero-dorsal top of the olfactory bulbs, and depending on the taxa it can variate in thickness (sometimes thinner at the center of the bulbs, sometimes the reverse).
I tried to search in the literature but I didn't find anything satisfying. I eliminated the possibility that these structures may be vomeronasal nerves (they originate at the dorsal top of the accessory olfactory bulb, so rather in the middle of the main olfactory bulb ; see Fig. 6 of for instance) or the terminal nerve (aka cranial nerve 0) that also runs more ventrally (see Fig. 7 of for instance).
Could anyone help me ? Even if you don't work on bats especially, but on other mammalian groups, any idea would be helpful.
Thanks a lot,
Jacob
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Hi Daniel,
Thanks for your answer, and for the references ! They are very interesting, beyond my question. I'll try to contact these authors.
That was a good idea, thank you ! ;)
Cheers
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Tropidacris collaris is a grasshopper. Adults, which can reach more than 15 of length, are strong flyers and actually they resemble small birds or bats. Most of the time T. collaris is found in treetops and tree trunks. No sampling technique has been proposed as far as I know. I am thinking on trying some known sampling technique tha has been applied for other insects or developing a specific one. Any suggestions? Could flight interception traps work (adapted for size)?
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The approach to sampling actually depends on the research question that you are attempting to answer. It this is a question of population size, then a modified sampling approach that is used for bats and birds (net interception) could be undertaken. If this is about insect behaviour associated with the environment then transect sampling/canopy sampling could be undertaken but you would need to design a new method
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It's generally recommended to use a different set of barcode when reusing flow cells due to issues of barcode carry over despite thorough washing of the flow cell when running another batch of samples. Does that limitation still apply when using different sample types?, i.e. say first run, I'll sequence SARS-CoV2 amplicons using barcode set1, then say for the next sequencing run, I'll do a shotgun metagenomic approach for say bat intestine nucleic acid extracts using the same set of barcodes. And if I do that, are there bioinformatics approaches to discriminate the reads carry over from the first sequencing run?
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Yes! You can do whatever you like, there are no rules :)
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Bat Conservation
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dear Anna,
in this issue "Migration Status of Bats in Ukraine" there is very interesting article by Tomasz Postawa "Migration activity of bats during hibernation"
Tomasz use loggers "OSNET". for details ask him: Tomasz Postawa
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Hello, I am a master student doing my master thesis on bats and zoonotic diseases, whereof we used Nobuto blood filter strips for a blood test.
I have found several articles which have used this method, but none who explain how the blood filter strips actually work. How are these paper strips able to preserve the RNA for up to 2 months in room temperature by drying the blood?
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Well check this out
Article Assessment of the RNASound RNA Sampling Card for the preserv... and https://aem.asm.org/content/aem/28/2/323.full.pdf and this might help to in case https://www.sterlitech.com/blog/post/nobuto-filter-strips-for-studying-wild-animal-populations
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Hi, I am working on an Industrial Process "Tennessee Eastman Process" and I need to train Neural Network model for its reactor part using BAT optimisation algorithm in MATLAB/Simulink.
MATLAB neural network app doesn't provide the option of selecting BAT learning algorithm while training the model.
Can anyone guide me or help me in finding the code that can show how to train ANN model using BAT algorithm?
I have attached the relevant paper which uses BAT algorithm to train ANN model.
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Hi,
I would like to record and analyze ultrasonic vocalization of rodent (rat and mouse) newborn pups, and I find Wildlife Acoustics Echo Meter Touch 2 bat detector and the Kaleidoscope Lite analyzer software. Do anybody tried this device to record rodent USV?
Thank you in advance!
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I do not have direct experience in that field, but do have experience using the Echo Meter Touch to record bats. And I have accidentally recorded rodent ultrasound in the past when monitoring for bats with other detectors. Assuming the newborn vocalizations are ultrasonic I see no reason you cannot detect them with the EMT. In the settings you would just want to set the low threshold filter low enough, and you would want to keep Noise files.
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Hi! I am working on bats. I am eager to mount low cost, light weight, long battery life navigation devices. I will also mount ibutton temperature and humidity sensor along with navigation device. The purpose of attaching navigation and temperature sensor to bat is to 1) measure temperature at the level of bat in the canopy of roost tree. 2). navigation device help recover temperature sensor in case temperature sensor is lost and also help track bats foraging areas. Requirement for temperature sensor and navigation device is 1) light weight (20-40gm) Measure temperature (-20 to+80) Humidity Measurement (95% RH to 100% RH) Extended battery life (>5 Years). I found out these devices Remora 2 (GPS 4G) https://www.digitalmatter.com/Devices/4G-GPS-Tracker-Devices/Remora2 and Ibutton Temperature Senor https://www.embeddeddatasystems.com/DS1923-F5--Hygrochron-Temperature-Humidity-iButton_p_101.html
Please suggest any better option for meeting my research objectives?
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Yeah! But they are quite expensive. I am looking for similar GSM/GPS devices with local network coverage.
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It is accepted that bats communicate with bats of the same species, and the frequency of this is indicative of the bat species. But can bats communicate with those from another species?
We are licensed to care for bats in the UK, and those who are in our care and that we are rehabilitating are common pipistrelles, apart from one other, which is a Natterer's bat. He is in a tank by himself but in the same room as the pipistrelles.
He will be aware of the presence of the pipistrelles, who clearly communicate between themselves, but it would be good to know if he could communicate with them to some degree. He will be with us for a bit longer; he still has a wing that is weaker than the other, and could not fly well enough when we tested him to allow him to survive in the wild. And winter (and hibernation) will be coming.
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follow
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I am doing an ectoparasitic diversity survey of bats in Texas, United States. Any taxonomic keys that might help me morphologically identify specimens is helpful.
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Is there anything here of help? We are licensed to work with bats in the UK, but although we see ectoparasites, they tend to reduce in number over time as we rehabilitate the bat and be not too problematic. Maybe you might find it helpful to contact the authors of the below publications?
This, by David Dodds, refers to the UK:
A Brief Guide to Bat Ectoparasites
This edition is dated 2018
Ritzi, C. M., Ammerman, L. K., Dixon, M. T., & Richerson, J. V. (2001). Bat ectoparasites from the Trans-Pecos region of Texas, including notes from Big Bend National Park. Journal of Medical entomology, 38(3), 400-404.
Reeves, W. K., Beck, J., Orlova, M. V., Daly, J. L., Pippin, K., Revan, F., & Loftis, A. D. (2016). Ecology of bats, their ectoparasites, and associated pathogens on Saint Kitts Island. Journal of Medical Entomology, 53(5), 1218-1225.
Very best wishes with this,
Mary
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It would be very appreciated if anyone can recommend a good identification key for ectoparasites in bats.
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I am doing a series of SDMs using MaxEnt for different species of bats, I am using different features and regularization values, in addition, I am using spatial partition and jackknife for cross validation.
I would like to start selecting the models according to their significance, and then continue with other metrics such as the omission rate and AIC in order to select one of the models.
I know that the partial ROC exists but I don't know if it can be done with the partitions that I am using (space partition and jackknife) or it can only be calculated with random non-space partitions.
That is the question
Thanks.
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You could just do AIC or BIC
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It is excellent news about the COVID-19 vaccination programme and the vaccination trials where both humoral and cellular immune responses to challenge, after vaccination are observed. These positive responses will be required to neutralise the virus before immune escape mechanisms can be triggered from viral mutation and evolution.
Another similar single stranded positive (+ve SS) virus (HCV) has a continuous emergence of resistant strains of virus in HCV, resulting from the quasispecies that evolve, resulting in escape mutations, due to a rapid change in viral proteins. In the case of HCV, the high level of genetic diversity of the virus hampers the vaccination progress, whereas in the case of SARSCoV-2, genetic variation has also been reported where the coronavirus accumulates about two changes per month but most do not affect the behaviour of the virus, however, one SNP causes a change on the spike from aspartic acid to glycine at position 641 of the coronavirus spike and this is thought to make the virus more transmittable or more severe. G641 variant is now more prevalent than the aspartic acid version, globally. This is a genetically distinct variant but closely related to other variants within the same genotype-designated quasi species. These successful members of the quasispecies arise as a result of weak conservation of sequence and high evolutionary selection. Highly changeable quasispecies, generated as a consequence of evolutionary pressure from human immune responses, such as from antibodies, should be taken into consideration when considering candidates for the vaccine trials.
Although we do not have any evidence that immune pressure causes RNA rearrangement or mutation of the COVID-19 genome, as often occurs in other virus species or between other species, however, it is clear that these events did take place zoonotically, resulting in the SARSCoV-2 emergence after jumping between different animal species. The existence of COVID-19 and ability to infect humans is evidence of this rearrangement through the process of zoonosis with bats, pangolin and civet cats. Frequent and rapid rearrangements in other chronic viral infections, such as, HCV often render therapeutics ineffective.
I am concerned that there may be selection pressure on the virus during the vaccination process resulting in escape variants that are even more resilient and transmittable than the current SARSCoV-2. Administering the vaccine to many immunologically naïve individuals could drive many more escape variants, forced to evolve due to immune pressure but I presume that virus genetic mutation will be monitored throughout the trials. (This process slightly reminds me of the RAG gene recombinant rearrangement activation of genes in antibody and T ell receptor differentiation but an unregulated mirror image of the process in the case of the virus, both achieving a ‘’best fit’ of the antigen). Thus, the immune response of entirely healthy individuals, that receives the vaccine, could drive viral mutation inducing unknown viral escape mechanisms that may impair recognition by human immune systems. The similar SS +ve RNA virus of HCV has rapid and frequent viral mutations.
Should scientists also be cautious about viral RNA rearrangement between different viruses and tread carefully as to which individuals are vaccinated, in case they have a chronic RNA virus that could rearrange with COVID-19? We do know that +ve SS RNA viruses mutate, like HIV and HCV and some of these viruses evolve through rearrangement of the RNA.
Could it be possible that the presence of HIV or HCV virus, as indeed many other virus strains, may enable a more dangerous evolution of escape quasi-species to arise? It was reported that the exclusion of HIV patients as vaccine recipients for COVID-19 has been described as discriminatory but perhaps it should be considered as cautionary, based on basic knowledge and understanding about the behaviour of viruses and its engagement with human immune systems. Anything that may possibly influence a high mutation rate, further enabling viral persistence by evading the innate, humoral and cellular immune responses, should be avoided. Scientists have already witnessed lymphopenia in severely affected patients due to the virus attaching to ACE2 on Th1 cytotoxic cells thus affecting the cellular response in severely affected patients. HIV attaches to CD4+ T cells via the CCR5 receptor also causing lymphopenia.
Should these factors be considered or endorsed in the selection of vaccine recipient candidates in this highly uncertain phase of experimentation, with the above considerations included in the exclusion criteria for the participants?
Do any others have any of these concerns, please discuss.
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Annwyne Houldsworth Thank you, agree with your points, this much high rate of mutation could possibly lead to antigenic sin, vaccination of protective immune system.
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I opened these results in Biovia Discovery Studio, bat I could get there only the length of the bond.
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As mention by @Martin Klvana you can use electrostatic force rule. Or you can cut out the interacting atom with ligand and save the structure and open this structure in gaussian. Then try to calculate. Give a try, I am not sure.
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Does anyone know a fast and simple method (apart of using tweezers) to sample ectoparasites (especially bat flies, Nycteribiidae, but also mites and ticks) on small bats (15 g)? We release the bats after sampling.
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Following
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Dear All,
The bat workers community in Belgium raised some questions on risks associated with bat work and Covid-19. These questions might be relevant to think about for many bat workers. Can you please comment on these or sent some references that deal with this matter?
The questions are:
Is there a risk that the Covid-19 causing CORONA virus will be transmitted from bat workers to local bat species? If yes, what are the potential consequences, towards bats or towards humans by creating a new reservoir?
Should we advise to temporarily stop all capturing of bats and if yes, for how long?
Should we advise to temporarily stop counting of summer roosts an eventually later on counting of hibernacula?
Will such measures have effects on research and monitoring / reporting schemes?
Thank you very much in advance for your time and for sharing your opinion with us,
Kind regards,
Ralf Gyselings
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Following.
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There is growing suspicion to the bats as the source of COVID-19 outbreak. It is also said that bats are the reservoirs of viruses and it may jump to humans at one point of time or the other. There are different cultures of eating bats to conservation across the globe. According to Bat Specialist Tigga Kingston, bats are important for human welfare and ecosystem health. They play critical roles worth billions of dollars as pollinators, seed dispersers and agents of pest suppression.
I am very much concerned about the practice of eating bats, rodents and ungulates as because I belong to an area where such practice is still prevalent. It is difficult to pass on such an message to the community who have age-old culture of eating these animals. At the same time looking at the facts that these animals are reservoirs of viruses I would say such practice should be stopped now. In fact, the boundary between humans and animals is diminishing nowadays due to destruction of habitats and the likelihood of viruses jumping from animals to humans will be remarkably high in the coming days. Inviting your opinions for this pertinent discussion.
I am uploading herewith the link of Down To Earth
COVID-19: ‘It is easy to blame bats than to look at ourselves’
Bat expert Tigga Kingston, Co-Chair of the Bat Specialist Group, at the International Union for Conservation of Nature’s Species Survival Commission, and professor, Department of Biological Sciences, Texas Tech University at Lubbock
Interview taken by By Rajat Ghai
Last Updated: Tuesday 05 May 2020
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Yes, because bats are known to harbour highly pathogenic viruses.
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Hello everybody!
I thought it would be interesting to start a discussion about bats and climate change. Thus, climate change belongs to the major threats and challenges to global bat conservation through increased temperatures, changes in precipitation patterns, more droughts and heat waves as well as stronger and more intense hurricanes.
It would be great if you could use this discussion to share your favorite or new publications on the topic!
Thank you in advance for your interest,
Yann
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Dear Dr.Yann,
Climate change particularly due to increasing temperature has impact of humans as well as animals including bats.We have published a review article on Climate change.
Dave, S., Dave, P. and Pal, M. 2015. The impact of climate change on emergence and re-emergence of human vector diseases. International Journal of Livestock Health 5: 1- 10.
One can easily download our publication from Research Gate and Academia.
With kind regards,
Prof.Dr.Mahendra Pal
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Recently a colleague raised a question in RG on the latest number of global bats, as a follow-up question to it, is there any existing database or list of the estimated number of bat species per country? I tried to scan the web many times and no luck for a complete list. I know it's challenging to have the exact number as there are taxa that remains unresolved and estimate numbers will do and will be useful in the future updating. Some colleagues have already provided their country database on the former question. I will compile the numbers (separated by families, genera) in single excel sheet and will provide on this thread. Thanks in advance!
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Honduras currently has 113 bat species, you can see our paper that came out last week here: https://zse.pensoft.net/article/51059/
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Determining exactly how SARS-CoV-2 transitioned from animals to humans is now a top priority for many researchers. Bats are known to be a reservoir host for SARS-CoV-2, but with no established means of transmission to humans. Pangolins are the most highly trafficked mammal in the world, with Malayan pangolins widely involved in illicit trade across Southeast Asia. The abundance of these animals illegally traded in wet markets across Wuhan (China) could account for the transmission to humans and the rapid initial spread of the SARS-CoV-2 virus.
Can Pangolins be the missing link in coronavirus transmission?
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Yes, pangolins are missing link in the transmission of SARS-CoV-2 from bats to humans.
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Currently GAC,PAC,resins and / or RO has been proved to help in disinfection by prodcuts control by reducing NOM prescursors in drinking water.
For the last 6 years we have been testing a method which apperas to be the BAT for TTHM control.
Using a ceramac membrane (0.1µ) in cross flow mode, we have found the the coagulant agent can be concentrated upto 1,500 ppm.
This enhanced enhanced coagulation scheme allows to reject a more broad range of dissolned organic content from the raw water
consequently less chlorine is used and less TTHM is formed, without he use of GAC,PAC,resins or RO.
Will appreciate your comments ,questions and annalysis in order to determine if this filtration technique has the potentia lto beconme the BAT for TTHM control in drinking water.
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Good Answer Amila Abeynayaka
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The other day I heard two professionals comment on vaccine development in India. One of them said that animals like hamsters, ferrets etc. usually used in Vaccine research are not available in India. This poses a problem to those who wish to do vaccine development.
I wonder if they have considered using bats. Someone has reported finding SARS-Cov-2 in bats in India.
Even if they are not accepted for approved testing of new vaccines, they could be helpful in the process of vaccine development in earlier stages.
As a first step, we could test bats for specific antibodies.
Srinivasan Ramani
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Bat models of SARS-CoV-2 are not suitable for developing a vaccine, and have not been used anywhere in the the world to date.
A look at this link tells you why?
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The evidence suggests that the Covid19 virus jumped from bats to humans.
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I agree with Dr. Sadanand Pandey
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I need to compare the biggest Chinese internet payment providers (we call them BAT - Baidu, Alibaba, Tecent) with their American counterparts (Google, Apple, Facebook, Amazon). Every suggestion will be helpful where can I find information describing on a regular basis market share in third-party payments, e-commerce of both groups. Some financial data ralated to both groups will be also very helpful. I need to compare both groups in as many aspects as possible.
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Within a span of 153 bases (positions 22925 to 23077), there are these six codons for the key amino acids of the Receptor Binding Domain (RBD, within the Spike protein) for COVID-19 (sequence MN908947) for its strong attachment into the ACE2 (Angiotensin-converting enzyme 2) of the lungs of humans and of cats. This is only one portion of some 80.23 % of similitude between the virus of bat that provided its backbone and the virus of pangolin (sequence MT084071) that provided the RBD and other sequences, plus another 5.23 % matching other viruses from other pangolins, to give a similitude of 85.46 % match of both viruses. More sequences will be analyzed, because some of them, like one that has only four matches other than itself in the Genbank (running from base 2164 to base 3393)...
Additional sequences to explore are:
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And, this is how the 3-D structure looks [putting they 5 of the 6 key Amino Acids, leaving out the sixth one that is the "Y" at position 505, why?], attaching itself both to the civet and to humans, with the statement of its authors saying: "Our decade-long structural studies on SARS-CoV have firmly shown that receptor recognition by SARS-CoV is one of the most important determinants of its cross-species and human-to-human transmissions", and twice in the two images, the authors also say: "Here, mutations were introduced to the RBD region... based on sequence differences between SARS-CoV and 2019-nCoV [the COVID-19]": https://web.archive.org/web/20200416213137/https://jvi.asm.org/content/94/7/e00127-20; so, here Chinese researchers from Wuhan say that they are doing these studies of the best ways of the RBD attaching to ACE2 for at least ten years [without reporting until after the fact, of course, the final version to go undetected, otherwise will be to shoot themselves on their own foot]!!! So, the morale of this history is that, if we all seek at all the rest of the engineered bio-weapons in the way that the authors of the previous article above, that of "The Proximal Origin of
SARS-CoV-2", which is the COVID-19, basically all can be passed as "natural" using their biased criteria. They basically are providing the way fo the manufacturers of biological weapons to pass "undetected" by the currently biased and "gullible" scientific community of the planet.
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I have attached the screen shot. In the table below It compares sequences between COVID-19, SARS-COV and Bat COV
Mismatch numbers 11 and 9, meaning that COVID-19 compared to the other two sequences has that many number of point mutations in the selected regions? and assays primers & probe are able to distinguish specifically those point mutations and fail to amplify incorrect template? if so why does the number decrease in other assay? those assays primers & probe give false positive?
Am I interfering it rightly.
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Xin Pin hi assays primers & probe is very sensitive and the errors are due to several reasons: 1. Lack of dedicated connection primers & probe ( no setup )2- Contamination in the reaction and.....
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I want to use the number of gunshots recorded near a bat roosting colony as a measure of poaching pressure on a tropical island environment. The units will be deployed in sets of 4-5 in a line around several locations. Hiden in trees to avoid detection. Ideally, the recorders would turn on at the first gunshot to record the other shots but continuous recording is also a possibility.
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In large cities, police have technology that can pinpoint gunshots, and even differentiate between fireworks and car backfires!
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I have data from bat colonies from 5 geographic locations. STRUCTURE has identified 4 clusters/populations and a reviewer for the manuscript I have written is requesting that I show Fst values for the populations suggested by STRUCTURE. As you will know STRUCTURE uses many runs and produces many Fst outputs. Does anyone know of an R package or other software, to collate the Fst values from multiple runs and calculate pairwise p-values please?
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I'm sorry, I'm quite late answering this, hope you already found a solution.
In my case I never used Structure Fsts, I prepare the dataset with an extra column with the Structure clusters (new populations suggested by Structure) to which each individual belongs. Then you can calculate plenty of statistics per clusters and check significant differences among them.
There is many software that will calculate the pair-wise Fsts with p-values, probably the easier to use (because it has GUI) is Arlequin.
You will need to transform the input file format, I recommend PGDSpider.
If you are going through R, the best package for that that I have found is StAMPP.
You will probably use Adegenet to import your input file to R, so take the chance and plot a PCR (color it by cluster) and calculate some other diversity indexes with DiveRsity, they can be very informative.
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There are various components that contribute to successful batsmanship. To name a few: psychology, performance optimization, nutrition, biomechanics, strength & conditioning, morphology, fitness, skills training, etc.
From a biomechanics / technical perspective, the batting technique in cricket consists of various elements such as the grip, stance, backlift, downswing, impact with the ball and follow through.
In your view, list three main components that define modern success of a batsman in cricket. What do you think contributes to successful batsmanship?
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Technique, concentration and mental toughness.
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Hi everyone
I'm trying to run a PERMANOVA to test if there's a significant difference in diet between pregnant/non-pregnant individuals of the Mauritian free-tailed bat. I've got an OTU-table (presence/absence, samples on the x-axis, OTUs on the y-axis) and a table with samples in the first row and pregnancy status (P/NP) in the second row. Both tables are in a tab-separated format.
I'm using the adonis function in the vegan package. I cannot make it work. My command line looks like this:
library(vegan)
otu_zeale <- read.delim("zeale_otu.txt")
pregnancy_zeale <- read.delim("zeale_pregnancy.txt")
adonis(otu_zeale ~ pregnancy_zeale, permutations = 1000)
Then it says "Error in model.frame.default(formula, data, drop.unused.levels = TRUE) :
invalid type (list) for variable 'pregnancy_zeale'"
What am I doing wrong?
Thank you!
Alex
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I am conducting hemi-nested One-Step PCR assay on rectal swabs from bats stored in RNAlater. In the first 300 samples, I got clear and beautiful gel. Recently, the gel picture became as the attached image. This was only after running the second PCR . I changed the primers, reagents, diluted the samples, but nothing succeeded.
Please, have any of you experienced such situation? and what I should do?
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What is your expected amplicon size? There is no band for the positive control. This suggests that some thing went wrong. Please check all your reagents and standardize your PCR with positive and negative controls.
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Dear researchers,
We are working in Colombia with the biological groups: primates and bats. We have been debating about landscape extent area/lenght, and also we have had read many articles about without knowing the exact criteria of researchers in this field.
We intend to measure focal primate species responses in landscape windows differentiated by configuration and composition. So it is very relevant to choose correctly the window size.
I really appreciate literature and fundamented opinions,
Regards!
Johana.
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Thank you, Andrew. ¿Do you have bibliography that can help us support your suggestion?. :)
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Hello everyone!
There is difference between songbird singing from different regions in the same country, and I guess, differences between songbirds from different countries.
BUT
Is there any difference, for a same bat species, between acoustic signals of two individuals living in separated region? Like, between a Spanish Pipistrellus pipistrellus and a Greek or Danish one?
This question may appears a bit dumb, but I don't find info about it. It may never have been discussed before, or already been discussed and determined, I don't know.
Hope you can enlighten me about this. :)
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Hi.
We know that in the Canary Islands there are some differences in the social calls of some bat species compared with nearby continental populations, like in the case of Hypsugo savii. Logically, this is a result of insular evolution.
Best regards.
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for its subsequent molecular and morphological identification.
Thank you
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Is anyone familiar with the Capillaria life cycle in bats? There are studies in bat endoparasites including Capillaria. However, it did not include the life cycle.
Thank you.
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I've conducted a bat survey at one of Tehran's Park in Iran, using Bat Logger. I use the own company software (BatExplorer). But I want to know if there are better softwares with higher accuracy and also what kind of analysis can be done with these softwares?
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Hi Danial, I think you can also use R for most purposes. I don't really know what kind of data you get from the bat logger, but if you are limited by license fees (and the embargo situation) and you get the proper data exports managed, you might want to look into using R. Jerome Sueur has also a book written for bioacoustic analyses in R (which he has been pioneering).
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Wonder why bats seem to be able to fight off Ebola virus without becoming ill themselves. Are EVEs involved in their special immune system?
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Please have a look at the following PDF attachment.
Thanks!
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Is the presence of ectoparasites common in European bats?
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Please take a look at the following reference.
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I am currently searching for an instrument for my thesis.
Im looking for the Biology Achievement Test.
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Piyush Kumar thank you..
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Using Matlab Simulink Model DC Controller and PID Model , I want to Implement BAT Algorithm First time in my Simulink Model , can anyone suggest the idea how to implement Design BAT Algorithm First time in Simulink Design?
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For the first time, you can search for the Bat Algorithm by Prof. Yang, and then call out the algorithm in Simulink using the Function Caller block or the MATLAB Function block. If you have trouble, you may contact the creator.
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Good day house.
Please I want to screen about 100 DNA samples extracted from bats' blood for Plasmodium and its relatives. For the PCR I need positive control. Is it right for me to extract DNA from a Malaria Parasite positive human blood sample as the positive control?
Thanks
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No, the species will be different, although some may be very close. A review of the literature may provide what you need, contact the authors and local zoology people. Good Luck with your research.
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This one does not require a red quale or a bat or any theory of thought. Consider raw, subjective pain (or pleasure). This is fundamentally, irreconcilably different from and unbuildable from information flows or any other cold, neutral concept. That is good news, because it quickly forces us to realize that pain may happen ultimately in tiny bits, but that those bits of pain are elemental in our universe, and 50 percent of consciousness, the affective part, from whence springs our will, comes to us quite directly from these elemental bits. As a large bonus, this then also suggests that our other basic feelings, that are not very negative or positive- the impact portion of perception and cognition that accompanies the information and turns it into a subjective experience- originates from a similar source.
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"long regarded most philosphical discussions as talking shops which are unlikely to produce much progress in understanding the mechansim of the mind until said phiosophers acquire a better understanding of physics"
Agree.
"...Leibnoz... amazoingly perceptive and well ahead"
Agree.
"advanced intuition"
Agree.
Regards, welcome.
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Hi,
I'm currently using Random Forests to classify acoustic data to corresponding bat species, however I was wondering if there is a way to also incorporate unsupervised cluster analysis into my classifier (such as K-Means or Hierarchical modelling)?
Whilst I have training data for ~40% of my target species, there is likely to be a large proportion of my field dataset which corresponds to species that haven't yet been described in the supervised model and therefore I would like for the classifier to be able to assign new clusters/call types to new classes (that hopefully can be matched to a species later).
I have been looking at consensus maximisation algorithms but I'm not sure if this would be the best method.
Any help is greatly appreciated!
(I'm not a computer scientist so please forgive me if this is a very straightforward question)
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What do you mean for semi-supervised learning?
Reinforcement learning?
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I am looking for a fractionation protocol for mouse adipose tissue (BAT and WAT)
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Hi Andrea,
i want to isolate protein from nuclei and cytoplasm of WAT and BAT. I need a fractionation protocol to seperate nuclei and cytoplasm and want to do a western blot with this samples. The problem is that with the protocol we use right now (which works nice for liver samples) we do not get enough protein (especially for the nuclei fraction) for WAT/BAT and we would need an adapted protocol to get a higher yield of protein and to get rid of lipids.
thanks a lot!
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I am actually using bat embryos, but they should behave similarly to mouse or other mammals. We have tried the staining before, but to no success. We think that the PFA may have created a barrier at the skin which prevents Alcian blue from entering the sample, because the stain works perfectly when it is sectioned.
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