Bacillaceae - Science topic

Honey is a sugar-rich product produced by Apis mellifera bees, with significant variability in properties due to the influence of geographic and climatic conditions and the predominant flora in the production region. Economically, beekeeping is an activity that generates profit and fulfills environmental and social functions, reinforcing the pillar...

Plant-associated bacteria play a crucial role in protecting plants from pathogens, yet the diversity and antagonistic potential of these bacteria across different plant species remain underexplored, especially in central Asia. To investigate the competitive dynamics between phytopathogenic fungi and plant-associated bacteria, we collected stem and...

We isolated seven endospore-forming bacteria from campus woodland and sequenced their genomes using Illumina NextSeq. We share the draft genome assemblies for strains Bacillus wiedmanii_SC129, Bacillus pseudomycoides_SC131, Bacillus pumilis_SC133, Peribacillus butanolivorans_SC135, Bacillus thuringiensis_SC136, Priestia megaterium_SC138, and Bacill...

Background Fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), larvae have the potential to significantly reduce maize yields. The FAW has become resistant to pesticides and environmental pollutants, making chemical management of the insect useless. Safe and efficient substitutes are desperately needed to manage FAW c...

Interrow cover crop implementation is considered a promising and sustainable agronomic practice for enhancing crop performance and soil health in agroecosystems, mediated by improvement of the soil microbiome. This study assessed how interrow cover crop implementation affected the microbial community in the soil, and may enhance its suppressiveness...

The present study aimed to investigate the diversity of bacterial communities in seleniferous soils using Illumina Mi-Seq Next-Generation Sequencing. This study also compared seleniferous soils (SE) with non-seleniferous (NS) soils to evaluate Selenium (Se) impact on microbial communities and soil properties. Metagenomic analysis identified Proteob...

The impact of short-term feeding of three distinct diets containing insect meals on the intestinal microbiota of African catfish hybrid (Clarias gariepinus × Heterobranchus longifilis) juveniles was examined. The animals received experimental diets containing 30% insect meals derived from black soldier-fly larvae (BSL) (Hermetia illucens), yellow m...

The intestinal microbiome contributes to colorectal carcinogenesis, disease progression, and response to therapy. Pathologic complete response is the therapeutic goal of neoadjuvant chemoradiation in rectal carcinoma. Nonoperative management has become an accepted strategy, and markers of complete treatment response are needed. Intestinal commensal...

Some amphibian species adapted to urban ecosystems may have altered cryptic interactions with microorganisms. In anurans, the skin bacterial microbiota plays a fundamental role in host protection, yet little is understood about its composition in relation to habitat conditions. The Venezuelan Snouted Treefrog, Scinax x-signatus, occurs in both natu...

The live microbiota of tea has not been extensively investigated. This study aimed to identify the live, culturable microbiota from four types of tea with varying oxidation levels, before and after brewing. Tea leaves and brews from oolong and fermented teas were analyzed for total viable counts of aerobic bacteria, lactobacilli, fungi, and Enterob...

This study is aimed at exploring the relationship between maternal weight categories, including pre-pregnancy body mass index (P-BMI) and gestational weight gain (GWG), and the composition of the infant gut microbiome in the early days of life. We recruited 71 mother-infant pairs from Kangwon National University Hospital and Bundang CHA Hospital, c...

Mangrove forests are intertidal ecosystems that harbor diverse microbial communities essential for biogeochemical cycles and energy flow. This study investigated the seasonal and spatial patterns of bacterial communities in the artificially introduced mangrove sediments of the Ao River estuary using 16S rRNA gene amplicon high-throughput sequencing...

Sustainable agriculture relies on effective soil management, making it crucial to assess soil health, especially in areas of agricultural expansion, such as the Cerrado in the MATOPIBA region. Sustainable strategies, such as integrated production systems (crop–livestock–forestry), are essential to mitigate these impacts. However, little is known ab...

Fermentation of organisms for recycling is important for the efficient cycling of nitrogen and phosphorus resources for a sustainable society, but the functionality of fermented products needs to be evaluated. Here, we clarify the anti-pathogenic properties for fish of a compost-type feed additive fermented by thermophilic Bacillaceae using non-edi...

Enantiopure (S)-2-methylbutanoic acid [(S)-2-MBA] is a high-value chiral compound with applications in fragrances, pharmaceuticals, and agrochemicals. However, conventional chemical synthesis lacks stereoselectivity, while existing biosynthetic methods suffer from low yield and purity. Here, we report a novel microbial process using Bacillus spiziz...

In vitro shoot culture and cryopreservation (CP) are techniques essential for the ex situ preservation of genetic resources and the production of plant propagation material of clonally propagated horticultural crops. Changes in plant-associated microbiota diversity and composition induced by in vitro cultivation and CP treatment could have a negati...

Background Accumulation of antibiotics in crops threatens human health. However, the mechanisms and effects of microorganisms on the uptake and accumulation of antibiotics in crops remain poorly understood. This study aimed to investigate the impact and underlying mechanisms of seed-borne microbiota in root on ciprofloxacin (CIP) accumulation in tw...

The yellow stemborer, Scirpophaga incertulas, is a monophagous pest of rice, attacking the crop from its vegetative to reproductive stages. Microorganisms are crucial in influencing the insect’s life cycle, evolution, and ecology, presenting an avenue for understanding and improving management strategies. Present research employed advanced next-gen...

Two Gram-stain-positive, aerobic, rod-shaped, and motile strains FJAT-53684 T and FJAT-54145 T were isolated from the mangrove sediment. They optimally grew at pH 8.0 and could tolerate NaCl up to 5% (w/v). The optimum temperature for growth of strains FJAT-53684 T and FJAT-54145 T were 35 and 40 ºC, respectively. The 16S rRNA gene sequence similar...

The gut microbiota of insects plays a pivotal role in digesting food, supplying nutrients, and synthesizing enzymes, particularly those capable of degrading lignocellulosic biomass—a key factor in waste management. In Karnataka, India, the larvae of Holotrichia serrata and Leucopholis coneophora are major crop pests. However, the potential of their...

Sclerotinia stem rot, caused by the necrotrophic fungus Sclerotinia sclerotiorum poses a significant threat to Egyptian clover production, especially in Northern India's growing regions. To explore sustainable management strategies, thirty Bacillus spp. isolates were screened in vitro for their antagonistic activity against S. sclerotiorum. Bacillu...

Sariyanti M, Adri PM, Wibowo RH, Nasution AA, Hidayah T, Sipriyadi. 2025. Molecular identification and antimicrobial activities of Bacillus subtilis MS-01 isolated from Indonesian ethnic food lemea. Biodiversitas 26: 1069-1074. The community in Bengkulu Province has a variety of traditional foods produced through fermentation, one of which is a bam...

Fluoride contamination in water sources represents a persistent and grave global issue with severe health implications. Microorganisms are known to demonstrate tolerance to various pollutants, enabling their survival in diverse and adverse environments. In this study, we aimed to identify potential bacterial isolates capable of resisting and removi...

El objetivo de la presente investigación fue caracterizar microbiológicamente seis biopreparados que se producen en la de Granja Agrobiológica Sol Vida en Pamplona a los que se les atribuyen efectos biofertilizantes, bioestimulantes y antagonistas. La investigación se desarrolló en los laboratorios de microbiología y bacteriología de la Facultad de...

A Gram-stain positive, long-rod-shaped, motile, and aerobic bacterial strain, designated 16A2ET, was isolated from mangrove sediment sample. The 16S rRNA gene sequence similarity analysis indicated that strain 16A2ET exhibited high similarity to Ornithinibacillus contaminans CCUG 53201T (98.2%), Ornithinibacillus bavariensis WSBC 24001T (98.1%), an...

Microorganisms linked to the grapevine, Vitis vinifera, can impact crop quality, plant growth, and human and plant health. This study examined the bacterial community structures of brined grapevine leaves (n = 56) taken from seven distinct regions in Türkiye using next‐generation sequencing technology. Investigations were also conducted into the sa...

Serangga Black Soldier Fly (Hermetia illuscens Linnaeus, 1758) telah lama dikenal sebagai organisme yang dipergunakan dalam pengolahan sampah organik dengan metode biokonversi. H. illuscens adalah spesies lalat berpenampilan ramping yang sering disalahartikan sebagai tawon. H. illucens diketahui dapat mencerna bahan organik menjadi sumber nutrisi y...

To support the role of insects as sustainable feed and food ingredients, evaluating their potential microbiological risk and safety is crucial. In this study, we investigated the presence of antimicrobial resistance (AMR) genes in selected live opportunistic pathogenic bacteria isolated during the rearing process from clinically healthy farm-reared...

High-temperature Daqu is crucial to Jiang-flavor Baijiu production in the Southern Sichuan–Northern Guizhou region of China. However, the complex interplay among microorganisms, enzymes, and metabolites in the Daqu from this region requires further investigation. This study compared four high-temperature Daqu samples from this region, analyzing the...

Bacillibactin (BB) is a microbial siderophore produced by Bacillus species. BB is biosynthesized from 2,3‐dihydroxybenzoic acid (2,3‐DHB), Gly, and L‐Thr by nonribosomal peptide synthetase (NRPS) enzymes DhbE, DhbB, and DhbF. The biosynthetic gene cluster (dhb) is also conserved in some strains of thermophilic genera, Geobacillus, Anoxybacillus and...

Background Constipation is a common digestive system disorder, which is closely related to the intestinal flora. Zhishi Daozhi decoction (ZDD) is a traditional Chinese medicine prescription used to treat constipation caused by indigestion. This study is to evaluate the efficacy of ZDD in treating constipation and to elucidate the underlying mechani...

Marine resources are attractive for screening new useful bacteria. From a marine sediment sample, we performed isolation and screening of bacterial strains in search of new bioactive compounds. HPLC and ESI-MS analysis indicated that the new bacterium, Lysinibacillus sp. CTST325 (NBRC 116944), produced a new peptidic compound, lysinibacin. Genome s...

Gut mucosal immunity of teleost is mainly governed by mucosa-associated lymphoid tissues (MALT) and indigenous microbiota on mucosal surfaces of gut tract, which can confer protection against pathogenic invasion. However, the probiotic features of bacterial isolates from gut tract of triploid cyprinid fish (TCF) were largely unclear. In this study,...

Microbes of low abundance (i.e., rare biosphere) are involved in the ability of the coral as holobiont, to respond and acclimatize to local and global environmental disturbances. However, no complete characterization of these important microbial assemblages has been conducted. Outlining these assemblages would greatly benefit our understanding of t...

Background and aims Numerous studies have demonstrated the enhancement effects of organic amendment additions on soil organic carbon (SOC) accumulation in agroecosystems. However, the effects of different organic amendment types on stable SOC formation through belowground inputs remain poorly understood, especially under stress conditions. This stu...

This study aimed to assess the impact of radish sprouts on the gut microbiota of healthy individuals. Radish sprout additives, subjected to short-term storage and steam treatment, were used to intervene in an in vitro culture of human gut microbiota. The influence of radish sprouts on the gut microbiota was evaluated by monitoring short-chain fatty...

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Background: Although many sugars are known antibacterial in higher concentrations (> 50% W/V) and a few at low concentrations too (≤ 1 mg/mL), most of the studies are limited to only a few reference isolates, therefore no concrete conclusion can be drawn. Methods: This study evaluated the antimicrobial potential of 19 sugars against 8000 isolates o...

This review synthesizes key findings from the past five years of experimental literature, elucidating the gut microbiome’s significant influence on the pathogenesis of thyroid diseases. A pronounced shift in the gut microbiota composition has been consistently observed, with a significant reduction in bacteria such as Bifidobacterium, Bacillaceae,...

Spores of Bacillaceae , ubiquitous in soil, can withstand extreme conditions virtually indefinitely. The spore coat, one of the most sophisticated multiprotein complexes built by bacteria, protects the spore from environmental stresses and predators. In Bacillus subtilis , assembly of the multilayered coat initiates at the forespore pole and is per...

Dibutyl phthalate (DBP) with teratogenicity, carcinogenesis, and mutagenesis, is a ubiquitous endocrine disruptor in the environment. The widespread usage of plastic mulch has resulted in a severe DBP pollution problem in agricultural soil. One of the most vital ways to mitigate the DBP pollution problem is to use DBP degrading bacteria to reduce t...

The diversity of gut microbiota is an important ecological resource that plays a key role in maintenance of the host homeostasis. It is extremely important to preserve the existing gut microbiota diversity, which ensures its resistance to the negative effects of abiotic factors, while the study of the antibiotic role in the disturbance of microbiot...

Sustainable management of organic waste, such as food waste (FW) and livestock manure (LS), is essential for reducing pollution and promoting resource recycling. This study investigated the effects of Bacillus sp. inoculation and biochar addition on composting efficiency, microbial dynamics, and physicochemical properties. Bacillus sp. accelerated...

Arboviruses transmitted by Aedes aegypti, such as dengue, Zika, and chikungunya, pose significant public health threats. The biological agent Bacillus thuringiensis (Bt) has been explored as an effective, safe, and economically sustainable method to reduce mosquito populations. This study aimed to identify Bt strains with potent larvicidal activity...

The study of the effect of the mechanism of urea addition to sewage sludge and sawdust-composting substrates on methane production is still limited. In the present study, the systematic investigation of the effect of urea addition (0.18, 0.9 and 1.8 kg) on methane production is discussed through the dynamics of physical properties, enzymes, and the...

Considering the dangerous effects on the environment of commonly used packaging materials like low-density polyethylene (LDPE), finding a practical and environmentally safe method for decomposing plastics is crucial. In this study, a bacterial strain (HMBC3) was identified in plastic-contaminated soil samples from eastern Saudi Arabia and showed po...

Aim. A comprehensive study on the antimicrobial properties of new 1,2,4-triazole derivatives using the tools of in silico and in vitro studies. Materials and methods. Computer search techniques were used to find a compound with a strong antibacterial activity; in silico molecular docking (receptors for class A (PDB id: 1n9b) and class A SHV-1 (PDB...

The use of microbial agents is an environmentally friendly approach to mosquito control, wherein natural enemies of mosquitoes are employed to control mosquito populations. A novel mosquitocidal bacterium was isolated from clay soil from the Union Territory of Puducherry, India, and was identified as Bacillus thuringiensis serovar israelensis (Bti)...

Aims Mixed cropping systems such as intercropping and crop rotation have been proven to be sustainable agronomic tools that provide agro-ecological services and improve crop yield through soil physical, chemical and biological changes in the soil. In this study, we aimed to assess the impact of different mixed cropping systems on a crop well-adapte...

Background In vitro cultivation and cryopreservation techniques are essential tools for genetic diversity conservation and pathogen-free plant propagation of horticultural crops. The optimisation of cryopreservation protocols typically focuses on minimising the negative effects of pretreatment with cryoprotectors (CPs), cryogenic freezing (CF) trea...

A comprehensive study on the antimicrobial properties of new 1,2,4-triazole derivatives using the tools of in silico and in vitro studies. Computer search techniques were used to find a compound with a strong antibacterial activity; in silico molecular docking (receptors for class A (PDB id: 1n9b) and class A SHV-1 (PDB id: 2zd8) beta-lactamase) an...

Uncultured microorganisms represent a promising and untapped source of antibacterial compounds, crucial in the fight against the significant threat of antimicrobial resistance (AMR). In this study, both traditional and isolation chip (iChip) cultivation techniques were employed to enhance the recovery of known and unknown microorganisms from soils...

Plant microbiomes represent a reservoir of adaptive traits that can enhance plant resilience and productivity. In this study, we investigated the potential of a synthetic microbial consortium (SynCom), composed of five nitrogen-fixing Bacillaceae derived from the phyllosphere of Pistacia lentiscus L., to promote the growth of Lactuca sativa L. unde...

Flowering plants can be introduced in modern agroecosystems to support resident natural enemies in the context of Conservation Biological Control (CBC). Buckwheat (Fagopyrum esculentum) (Polygonales: Polygonaceae) has been shown to enhance the longevity of several parasitoids through the provision of high quality and easily accessible floral nectar...

Background and aims Selenium (Se) deficiency in soil and human diets may worsen with elevated atmospheric carbon dioxide (eCO2). However, current research focused on essential nutrient elements, such as nitrogen, phosphorus and potassium, the effects of eCO2 on Se accumulation in staple crops are understudied. Here, the Se-hyperaccumulator Cardamin...

Heavy metal pollution, particularly cadmium, poses severe environmental and health risks due to its high toxicity and mobility, necessitating effective remediation strategies. While both microbially induced carbonate precipitation (MICP) and humic acid adsorption are promising methods for heavy metal mitigation, their combined effects, particularly...

Microbially induced calcite precipitation (MICP) offers promising solutions for sustainable, low-cement infrastructure materials. While it is known how urea catabolism leads to biomineralization, the non-ureolytic pathways of MICP are less clear. This limits the use of the latter in biotechnology, despite its clear benefit of avoiding toxic ammonia...

Background: Foil, cellophane, and disposable plastic containers are commonly employed in food packaging, namely as coatings on food items. Nevertheless, the existence of microbes on the surface and inside the internal composition of these materials gives rise to concerns, as they have the capacity to multiply under ideal circumstances and spread to...

Topik ini membahas terkait kelompok bakteri Bacillus (selain B. anthracis). Bakteri Bacillus yang dibahas yakni B. cereus (paling utama), kemudian B. subtilis dan B. thurigiensis. Pembahasan B. cereus dibuat lebih mendalam dengan pembahasan meliputi data epidemiologi, faktor virulensi, spektrum klinis, diagnosis laboratorium hingga terapi.

Black soldier fly larvae (BSFL) have attracted attention due to their ability to upcycle various biological side streams into valuable biomass, such as proteins, lipids, and chitin. In this study, we investigated the impact of high-fiber diets on larval growth performance and the shift of microbes in the gut. We tested empty fruit bunches (EFB), po...

An isolate of a rod-shaped, endospore-forming Gram-positive facultative anaerobe was recovered from infected orthopaedic implant samples collected from a hospital in Islamabad, Pakistan. The isolate was motile and displayed optimal growth at 37°C (pH 7.0). The whole genome sequence of strain showed 97% similarity with the Bacillaceae family and 90%...

Domibacillus sp. 8LH is a whitish bacterium isolated from the pools of the Cuatro Cienegas Basin (CCB) in the state of Coahuila and belongs to the Bacillaceae family. It grows in circular colonies of about 6 mm in diameter and is capable of forming biofilms. This strain was identified because, in previous experiments in our laboratory, it presented...

Background Issues associated with proton pump inhibitor (PPI) usage have been documented. PPIs affect the gastrointestinal microbiome, as well as the saliva microbiota of healthy individuals. However, the alterations in the saliva microbiota of laryngopharyngeal reflux (LPR) patients remain unclear. This study aims to examine the composition of sal...

Utilizing coastal land for agriculture presents challenges such as low water content, high soil salinity, and low organic compound content. To support plant growth under these conditions, biofertilizers composed of plant growth promoting Rhizobacteria (PGPR), especially those inhabiting coastal areas, are needed. The Parangkusumo sand dunes on the...

The diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae), is a key pest of Brassicaceae. Chemical insecticides are the main method used for P. xylostella management, however, the moth has developed resistance to many insecticides, including biological agents such as Bacillus thuringiensis Berliner (Bacillales: Bacillaceae) (Bt) produ...

Microbially induced calcium carbonate precipitation (MICP) is a soil remediation method that has emerged as a viable and long-term solution for enhancing soil mechanical qualities. The technique of MICP that has been extensively researched is urea hydrolysis, which occurs naturally in the environment by urease-producing bacteria as part of their fu...

Tunicate maize (Zea mays var. tunicata A. St. Hil) is a landrace that constitutes a fundamental aspect of the socio-cultural identity of Ixtenco, Tlaxcala (Mexico) and represents an exotic phenotype whose kernels are enclosed in leaflike glumes. Despite multiple studies conducted worldwide on plant growth-promoting-rhizobacteria (PGPR) in commercia...

Purpose This study describes a pseudo-outbreak of Bacillaceae spp. bloodstream infections that spanned five months starting in May 2023 and the infection prevention measures implemented to control it. Methods This retrospective study was conducted at a tertiary infectious disease hospital in Bucharest, Romania. An observational audit of the blood...

Across the United States, melons are a high demand crop reaching a net production of 2.7 million tons in 2020 with an economic value of $915 million dollars. The goal of this study was to characterize the bacterial diversity of cantaloupe rinds and soil from commercial melon fields at the point of harvest from two major production regions, Arizona,...

Metagenome-assembled genomes (MAGs) have contributed to identifying non-culturable microorganisms and understanding their ecological functions. MAGs offer an advantage in investigating sporulation-associated genes, especially given the difficulty of isolating many species residing in the gut microbiota of multiple hosts. Bacterial sporulation is a...

Background Along bacteria, yeasts are common in forages and forage fermentations as spoilage microbes or as additives, yet few studies exist with species-level data on these fungi’s occurrence in feedstuff. Active dry yeast and other yeast-based products are also common feed additives in animal husbandry. Here, we aimed to characterize both ferment...

One bacterial strain, designated as C22-A2 T , was isolated from Lake LungmuCo in Tibet. Cells of strain C22-A2 T were long rod-shaped, Gram-stain-negative, non-spore-forming, with positive catalase and oxidase activity. Optimal growth occurred at 20–25 °C, pH 8.0 and with 3.0–7.0% (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene and whole genome...

The gut microbiota of insects plays a crucial role in digesting food, providing nutrients, and synthesizing enzymes. This approach is particularly relevant for degrading lignocellulosic biomass and managing waste. In Karnataka, the larvae of Holotrichia serrata and Leucopholis canephora are major crop pests, but the role of their bacterial communit...

Pectinase accelerates hemp retting. An elevated temperature during the enzymatic action is favorable for bacterial colonization. Industrial hemp (Cannabis sativa L.) bast fiber was retted in a 40 °C water bath under four different conditions: water retting, pectinase retting, bacterial retting, and bacterial retting with the presence of pectinase....

The Lactobacillales (LB) stand apart among bacterial orders, using manganese (Mn) instead of iron to support their growth and swiftly ferment complex foods while acidifying their environment. The present work investigates whether a shift in the use of Mn could mark the origin of LB. Transmembrane carriers of the ubiquitous Slc11 family play key rol...

We present the genomic sequences of 10 spore-forming bacteria from the Bacillaceae family isolated from fecal samples of mice residing in the Tisch Family Biblical Zoo, Jerusalem. These isolates suggest Bacillus bacteria are a native component of rodent gut flora, facilitating further research into gut colonization and microbiome diversity.

Soil microbiomes are key regulators of biogeochemical cycles and possess essential roles in ecosystem functions, particularly in arid environments. One beneficial function of various edaphic microbes is the ability to participate in Microbial Induced Calcite Precipitation (MICP). MICP is a biomineralization process extensively investigated as a soi...