Science topic

Autophagy - Science topic

The segregation and degradation of damaged or unwanted cytoplasmic constituents by autophagic vacuoles (cytolysosomes) composed of LYSOSOMES containing cellular components in the process of digestion; it plays an important role in BIOLOGICAL METAMORPHOSIS of amphibians, in the removal of bone by osteoclasts, and in the degradation of normal cell components in nutritional deficiency states.
Filters
All publications are displayed by default. Use this filter to view only publications with full-texts.
Publications related to Autophagy (10,000)
Sorted by most recent
Article
Full-text available
Vein grafts are widely used for coronary artery bypass grafting and hemodialysis access, but restenosis remains the "Achilles' heel" of these treatments. An extravascular stent is one wrapped around the vein graft and provides mechanical strength; it can buffer high arterial pressure and secondary vascular dilation of the vein to prevent restenosis...
Article
Full-text available
Background and Objectives Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease of unknown etiology and poorly understood pathophysiology. There is no specific biomarker either for diagnosis or prognosis. The aim of our study was to investigate differentially expressed proteins in the CSF and serum from patients with ALS to determine t...
Article
Full-text available
Hydrogel patch-based stem cell transplantation and microenvironment-regulating drug delivery strategy is promising for the treatment of myocardial infarction (MI). However, the low retention of cells and drugs limits their therapeutic efficacies. Here, we propose a prefixed sponge carpet strategy, that is, aldehyde-dextran sponge (ODS) loading anti...
Article
Full-text available
A novel sprayable adhesive is established (ZnMet-PF127) by the combination of a thermosensitive hydrogel (Pluronic F127, PF127) and a coordination complex of zinc and metformin (ZnMet). Here we demonstrate that ZnMet-PF127 potently promotes the healing of traumatic skin defect and burn skin injury by promoting cell proliferation, angiogenesis, coll...
Article
Full-text available
MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are two relevant classes of non-coding RNAs (ncRNAs) that play a pivotal role in a number of molecular processes through different epigenetic regulatory mechanisms of gene expression. As a matter of fact, the altered expression of these types of RNAs leads to the development and progression of a...
Article
Full-text available
Macroautophagy/autophagy is a process that degrades unnecessary cellular components to maintain homeostasis. The Ca²⁺-sensing protein CALM (calmodulin) is needed for many cell functions yet is a limiting factor because of its insufficient availability for its target proteins. Although Ca²⁺ produces diverse actions in autophagy, the role of CALM in...
Article
Full-text available
The hippocampus is a vulnerable structure that can be affected by many damaging stimuli, contributing to the development of diabetic cognitive dysfunction. One of these stimuli is autophagic dysregulation. Although autophagy is a physiological process that occurs at a basal level and is considered a pro-survival mechanism, autophagic dysregulation...
Article
Full-text available
Phenolic compounds derived from olive oil have beneficial health properties against cancer, neurodegenerative, and metabolic diseases. Therefore, there are discrepancies in their impact on mitochondrial function that result in changes in oxidative capacity, mitochondrial respiration, and energetic demands. This review focuses on the versatile role...
Article
Full-text available
Macroautophagy/autophagy initiated by STING1 (stimulator of interferon response cGAMP interactor 1) is actively involved in viral infection; and it is known that interferon-inducible guanylate-binding proteins (GBPs) exploit autophagy to defend the host against bacterial infection. Here we showed that in the tree shrew (Tupaia belangeri), a close r...
Article
Full-text available
Context Yunpi Heluo (YPHL) decoction is a Chinese herbal formula with particular advantages for treating type 2 diabetes. Yet, its exact mechanism of action is not fully understood. Objective To examine the therapeutic effect of YPHL on ectopic lipid deposition (EDL) in Zucker diabetic fatty (ZDF) rats and the underlying mechanism. Materials and...
Article
Full-text available
Context Aucubin (AU), an iridoid glycoside that is one of the active constituents of Eucommia ulmoides Oliv. (EUO) (Eucommiaceae), a traditional Chinese medicine, has been extensively studied in the management of neurological diseases (NDs). However, a comprehensive review of its effects and mechanisms in this regard is currently not available. Ob...
Article
Full-text available
Context Cyanidin has been shown to have therapeutic potential in osteoarthritis. However, it is unclear whether cyanidin prevents the progression of intervertebral disc degeneration (IVDD). Objective This study evaluates the effects of cyanidin on IVDD in vitro and in vivo. Materials and methods Nucleus pulposus cells (NPCs) isolated from lumbar...
Article
Full-text available
The process of LC3-associated phagocytosis (LAP) combines elements of phagocytosis and autophagy. LAP is involved in clearance of pathogens and cell debris, antigen presentation, and immune signaling. However, the mechanistic role of LAP is incompletely understood. Based on observations from our recent study, we propose that LAP might serve as a so...
Article
Full-text available
Equilibration of phospholipids between the two monolayers of the lipid bilayer of cellular membranes is mediated by scramblases acting as phospholipid shuttling proteins that are critical for cellular function, particularly during inter-organelle contact. Recent work has identified several protein scramblases, including TMEM41B, VMP1 and ATG9 that...
Article
Full-text available
Dysfunction of macroautophagy/autophagy has been demonstrated to contribute to multiple fibrotic diseases. In a recent study, we show that lnc-PFAR, a fibrotic-related lncRNA, is upregulated in human chronic pancreatitis tissues and mouse models and can serve as a biomarker for pancreatic fibrosis detection in the clinic. Indeed, our data reveal th...
Article
Full-text available
DNA damage-inducible transcript 3 (DDIT3), a transcription factor, is typically involved in virus replication control. We are the first to report that DDIT3 promotes the replication of bovine viral diarrhea virus, an RNA virus, by inhibiting innate immunity. However, whether the DDIT3 gene participates in DNA virus replication by regulating innate...
Article
Full-text available
Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC), but the role of the RhoA-YAP1-autophagy pathway in HCC remains unclear. Due to the core role of liver cancer stem cells (LCSCs) in HCC metastasis and recurrence, we explored the RhoA-YAP1-autophagy pathway in LCSCs under FSS. Our results indicate that LCSCs have st...
Article
Full-text available
Chaperone-mediated autophagy (CMA) is a unique proteolytic pathway, in which cytoplasmic proteins recognized by HSPA8/Hsc70 (heat shock protein 8) are transported into lysosomes for degradation. LAMP2A (lysosomal-associated membrane protein 2A) recruits the substrate/chaperone complex to the lysosome membrane. The structure of LAMP2A comprises a la...
Article
Full-text available
The pathogenesis of intestinal inflammation involves a complex network of cell signaling pathways. CGAS (cyclic GMP-AMP synthase) is a cytoplasmic DNA sensor that is most known for upregulating interferon-mediated inflammation. In this punctum, we discuss our novel finding that in the intestinal epithelium, CGAS binds to BECN1 (beclin 1) to subsequ...
Article
Full-text available
In plants, macroautophagy/autophagy is an essential mechanism responsible for a large variety of processes throughout the plant’s lifecycle, including nutrient processing, immunity, stress responses and senescence. Previous studies had observed the presence of autophagosomes in an Arabidopsis sexual reproduction system that prevents self-fertilizat...
Article
Full-text available
Some cervical cancer patients show poor response to neoadjuvant chemotherapy. Although it has been demonstrated that macroautophagy/autophagy contributes to the poor chemosensitivity, the underlying mechanism has not yet been fully clarified. In this autophagy punctum, we discuss our recent findings about the mechanism of SOX6-induced autophagy and...
Article
Full-text available
The anticancer immune response is shaped by immunogenic cell stress and death pathways. Thus, cancer cells can release danger-associated molecular patterns that act on pattern recognition receptors expressed by dendritic cells and their precursors to elicit an antitumor immune response. Here, we investigated the impact of single nucleotide polymorp...
Article
Full-text available
The accumulation of misfolded proteins and fibrillar aggregates inside neurons is a hallmark of several neurodegenerative diseases. While the exact role of these aggregates is still controversial, they are part of a cascade of molecular events that underlies the neuronal dysfunction and degeneration observed in these disorders. In this line, multip...
Article
Full-text available
In our recent study, we reported the molecular mechanisms of SARS-CoV-2 assembly and budding. Envelope protein (E) and membrane protein (M) of SARS-CoV-2 form complexes that ensure the uniform size of viral particles for viral maturation and budding. The E3 ligase RNF5 mediates the ubiquitination of M at residue K15 and thus enhances M-E interactio...
Article
Full-text available
Many zinc transporters have been found to play important roles in human cancers, but the detailed regulatory mechanism have yet to be well identified. Recently, utilizing Drosophila tumor models, we showed that ZnT86D/dZnT7, a zinc transporter localized on the Golgi apparatus, functions as a negative regulator during tumorigenesis. ZnT86D/dZnT7 RNA...
Article
Full-text available
Cell survival depends on dynamic interactions among the signaling pathways that control the endoplasmic reticulum (ER) stress response, macroautophagy/autophagy and apoptotic cell death. Our recent study reported an association of cytoplasmic SQSTM1/p62-mediated autophagy with disease progression and therapy resistance in head and neck squamous cel...
Article
Full-text available
Macroautophagy/autophagy is an adaptable pathway involved in the degradation of very different targets that include proteins, organelles, or even invading intracellular microorganisms. The regulation of this complex pathway depends on a great number of proteins, some common for the majority of the processes and others specific for a particular auto...
Article
Full-text available
Macroautophagy/autophagy is coupled to a myriad of intracellular processes, among which vesicular trafficking is an important contributor of membranes and proteins required at various stages, from autophagosome formation to degradation. Hence, understanding how membrane trafficking is coupled to autophagy induction and how cells rewire trafficking...
Article
Full-text available
Human APOE4 (apolipoprotein E4 isoform) is a powerful genetic risk factor for late-onset Alzheimer disease (AD). Many groups have investigated the effect of APOE4 on the degradation of amyloid β (Aβ), the main component of plaques found in the brains of AD patients. However, few studies have focused on the degradation of APOE itself. We investigate...
Article
Full-text available
TFEB (transcription factor EB) is essential for the upregulation of gene expression required for macroautophagy/autophagy and lysosomal function. Under nutrient-rich conditions, TFEB is inactivated by MTOR (mechanistic target of rapamycin kinase) complex 1 (MTORC1)-dependent phosphorylation on the lysosome. This suppresses TFEB activity via prevent...
Article
Full-text available
TFEBexplorer is a comprehensive web-tool to study genes modulated by the transcription factor EB (TFEB), a master regulator of lysosomal biogenesis and autophagy, that integrates multiple datasets following genetic perturbation of TFEB with experimentally validated and bioinformatically-predicted binding sites across multiple species. Given one gen...
Article
Full-text available
Context Jiedutongluotiaogan formula (JTTF), a traditional Chinese medicine (TCM), could promote islet function. However, the potential effect of JTTF on endoplasmic reticulum stress (ERS) and autophagy have not been reported. Objective This study explores the potential effect of JTTF on ERS and autophagy in the pancreas. Materials and methods The...
Article
Full-text available
Inorganic phosphate (Pi) is one of the most important nutrients for plant growth. Under Pi starvation, intracellular components are degraded for Pi recycling in plant cells. Macroautophagy/autophagy is a process for vacuolar degradation of cytoplasmic components including organelles, but it is still unclear whether this process is involved in plant...
Article
Full-text available
A significant number of follicular lymphoma patients display recurrent mutations in subunits and regulators of the vacuolar-type H⁺-translocating ATPase (V-ATPase). Past studies focusing on the role of these mutations highlighted essential functions of macroautophagy/autophagy, amino-acid, and nutrient-sensing pathways in the pathogenesis of this d...
Article
Full-text available
Cartilage is a crucial component of the developing and functioning skeleton. It establishes a template for bone formation and comprises the articular cartilage for smooth joint movement. It is formed by dedicated matrix-secreting cells (chondrocytes) whose development and survival are negatively affected by disorders of crucial homeostatic and meta...
Article
Full-text available
Autophagic cell death is characterized by uncontrolled macroautophagy/autophagy overactivation. Yet, the key modulators involved in autophagic cell death remain underexplored. In this study, PURPL (p53 upregulated regulator of p53 levels) is identified to act as an oncogene in promoting cell proliferation, colony formation, migration, invasiveness...
Article
Full-text available
Autophagy (in the form of macroautophagy) is the major intracellular protein quality control system for removal of damaged organelles and abnormally aggregated proteins. We and others have shown that dysregulated autophagic pathways contribute to accumulation and spread of misfolded proteins in many neurodegenerative disorders, including Parkinson...
Article
Full-text available
Mammalian STX17 (syntaxin 17) plays different cellular roles, including in mitochondrial fission, lipid droplet expansion and macroautophagy/autophagy, depending on the nutritional status. STX17 has a long C-terminal hydrophobic domain (CHD) with a hairpin-like structure, flanked by a basic amino acid-enriched C-terminal tail (C-tail). STX17 is pre...
Article
Full-text available
In a recently published study, we described a genetic interaction and functional cooperation between Atg7, an essential autophagy gene, and Palb2, a breast cancer susceptibility gene, in the maintenance of mitochondrial function, redox homeostasis, and neuronal viability. Our studies uncovered a new function for PALB2 in mitochondrial regulation an...
Article
Full-text available
sABSTRACT TANK-binding kinase 1 (TBK1), a noncanonical member of the inhibitor-kappaB kinases (IKKs) family, plays a vital role in coordinating the signalling pathways of innate immunity, involving in the process of neuroinflammation, autophagy, and oncogenesis. In current study, based on rational drug design strategy, we discovered a series of 1H-...
Article
Full-text available
Cell detachment from the extracellular matrix (ECM) typically promotes cell death via a form of apoptosis known as anoikis. However, in tumor cells, detachment can also induce cell survival, utilizing a process known as macroautophagy/autophagy, which involves degradation and removal of apoptotic proteins as well as rewiring of metabolic pathways s...
Article
Full-text available
Background To prove the internal connection, we deciphered the effect of cinnamaldehyde on kidney senescence through establishing animal and cell models. Methods In vivo, a rat senescence model was constructed using D-galactose (D-gal), and the modeled rats were further treated with cinnamaldehyde. In vitro, rat renal tubular epithelial cells (NRK...
Article
Full-text available
Context Xinbao pill (XBW), a traditional Chinese herbal formula, is widely used in clinical treatment for cardiovascular diseases; however, the therapeutic effect of XBW on myocardial ischaemia–reperfusion injury (MI/RI) is unclear. Objective This study evaluates the cardioprotective effect and molecular mechanism of XBW against MI/RI. Materials...
Article
Full-text available
The elimination of organelles to create the lens organelle-free zone (OFZ) is a macroautophagy/autophagy-dependent mechanism that occurs without causing cell death. This process is essential to the ability of the lens to focus incoming images on the retina, as the presence of these organelles would interfere with lens refraction of light and impair...
Article
Full-text available
Large numbers of point mutations in SQSTM1/p62 have been identified in amyotrophic lateral sclerosis (ALS) and frontotemporal degeneration (FTD). SQSTM1 interacts with ubiquitinated proteins, undergoing liquid-liquid phase separation, and the resulting SQSTM1-droplets are degraded by macroautophagy/autophagy. SQSTM1 also serves as a multiple signal...
Article
Full-text available
Protein quality control (PQC) is a conformational surveillance system critical to maintaining native protein composition in the cell. However, PQC mechanisms at the endo-lysosomal pathway especially toward membrane proteins and during cumulative endo-lysosomal stress are incompletely understood. We recently identified the ubiquitin ligase UBR1 as a...
Article
Full-text available
Alzheimer disease (AD) is a devastating condition associated with loss of proteostasis control and autophagy failure, leading to progressive neuronal cell death. However, the relationship between the activity in macroautophagy (MA) and chaperone-mediated autophagy (CMA) in the context of cargo clearance remains poorly dissected. We have found that...
Article
Full-text available
In this study, we investigated the effect of Solanum nigrum aerial parts (SNAP) on macrophage activation and macrophage autophagy in RAW264.7 cells. SNAP increased the production of immunostimulatory factors and phagocytosis in RAW264.7 cells. TLR4 inhibition blocked SNAP-mediated production of immunostimulatory factors. In addition, the JNK inhibi...
Article
Full-text available
In the final critical step for autophagic degradation, lysosomes fuse with autophagosomes to form autolysosomes. Although recent research has suggested that soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins are important for lysosome-autophagosome fusion, neither the architecture of the prefusion state nor the r...
Article
Full-text available
Acetaminophen (APAP), a widely used analgesic drug, is safe at therapeutic doses, but can produce significant hepatotoxicity upon overdose. Emerging evidence suggested that endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) are key mechanisms in APAP-mediated hepatotoxicity. While ER stress-UPR and macroautophagy/autophagy ap...
Article
Full-text available
The ability to adapt the proteolysis rates based on fluctuations in essential amino acid (EAA) availability is essential for life. The GCN2-eIF2α-ATF4 signaling pathway is involved in the adaptive response to EAA deprivations. Our previous results demonstrated that activation of this pathway is involved in upregulating the expression of many autoph...
Article
Full-text available
Well, because you ask that question, we are going to attempt to explain exactly why we do indeed need another journal focused on autophagy. If you are reading this far, you presumably know what “autophagy” means, so we do not have to impress upon you the importance of this topic, and how autophagic dysfunction is associated with numerous diseases i...
Article
Full-text available
Tumor treating fields (TTFields) is a novel, noninvasive regional antimitotic treatment modality approved for use in newly diagnosed glioblastoma (GBM). Nowadays, it is an integral part of the standard management of GBM. TTFields therapy delivers intermediate frequency (100-300 kHz), low intensity (1-3 V/cm), alternating electric fields to the tumo...
Article
Full-text available
Cell growth and differentiation signals of insulin-like growth factor-1 (IGF-1), a key regulator in embryonic and postnatal development, are mediated through the IGF-1 receptor (IGF-1R), which activates several downstream pathways. The present study aims to address crucial organogenesis and development pathways including Akt, MAPKs, heat shock resp...
Article
Full-text available
Semicarbazide (SMC), also known as carbamoyl hydrazide, is a key intermediate for the organic synthesis of drugs, pesticides, and a panoply of other applications. It is also regarded as a landmark metabolite of nitrofurazone, a banned veterinary drug. SMC produced in different ways will eventually enter the ocean and become an emerging marine pollu...
Article
Full-text available
Lysosomes are known to influence cholesterol trafficking into endoplasmic reticulum (ER) membranes. Though intracellular cholesterol levels are known to influence the lipid biosynthetic responses in ER, the specific effects of lysosomal modulation on these outcomes is not known. To demonstrate this, C2C12 cells were treated with chloroquine, a lyso...
Article
Full-text available
Background: Rab37-mediated exocytosis of tissue inhibitor of metalloproteinase 1 (TIMP1), an inflammatory cytokine, under serum-depleted conditions which leads to suppression of lung cancer cell metastasis has been reported. Starvation is also a stimulus of autophagic activity. Herein, we reveal that starvation activates Rab37 and induces autophag...
Article
Full-text available
Breast cancer cells abnormally express vasopressin (AVP) and its receptors. The effect of AVP is largely orchestrated through its downstream signaling and by receptor-mediated endocytosis (RME), in which Dynamin 2 (Dyn2) plays an integral role in vesicle closure. In this work, luminal A breast cancer cells were treated with AVP, and then Dynasore (...
Article
Full-text available
Protein arginine N-methyltransferases (PRMTs) have emerged as important actors in the eukaryotic stress response with implications in human disease, aging, and cell signaling. Intracellular free methylarginines contribute to cellular stress through their interaction with nitric oxide synthase (NOS). The arginine-dependent production of nitric oxide...