Questions related to Assisted Reproductive Technology
Recent evidences suggest that mitochondria play important roles in determining the quality and fate of the oocytes in several mammalian species. Changes in shape, number as well as the distribution pattern of mitochondria alter oocyte physiology. More scientific discussion and suggestions are needed in order to develop a new biomarker for for the improvement of assisted reproductive technology outcome.
I want to culture oocytes in vitro and analyze gene expression from primordial follicles and primary follicles in mice, but am unsure how to collect isolated oocytes and granulosa cells. So far I've only seen articles with protocols involving hyaluronidase and mechanical methods for denuding the oocyte. I want to isolate the oocytes from granulosa cells in mice. Does anyone have any particular methods that they could recommend to me please
As we know researches are being rapidly evolved in most majors. I would like to know where animal reproduction physiology researches will go to in future. where do animal reproduction physiologist see themselves in 5 to 10 years later?
In my opinion, it is vital for researchers in this field to find out which aspects of their major define future studies and accordingly researchers should empower their knowledge in growing areas.
It is my pleasure to know your idea about the future of animal reproduction physiology researches and related areas.
I would appreciate if any related papers, sites and other stuffs be suggested.
Does anyone know a reliable methodology to estimate the initial values of theta1 (or A) and theta2 (or B) in a non linear model? For example, in a logistic model describing scrotal circumference growth:
SC = A/[1 + B exp(-kt)]
How to reliably estimate the initial values of "A", "B" and "k"?
I'm using NLIN Procedure on SAS.
Hello everyone! I'm now a biomedical senior and get stuck selecting a research idea for my Bachelor thesis, especially, I'm very enthused in the field of Assisted Reproductive Technology. Actually, I don't know how to start, I mean the first steps of getting an idea. I would be grateful for all of your supports, thank you!!
India is becoming a hub of suurogacy outsourcing capitaL OF THE wORLD.Commercial surrogacy,egg donor programmes are becoming popular services provided by the fertility industry.Through lights on deeply regarding the issues.Well articulTED Suugestions are highly accepted.
Md Osim Aquatar
Could the transfer of embryos (or oocytes and zygotes) between different culture media induce alterations in their cytoskeletal structure leading to cell division failure?
Thanks in advance.
We are performing a superovulation and embryo collection programme in goats. We use commercial porcine FSH (Folltropin) and after dilution we store it for a maximum of 4 days at 4C. Could we freeze this FSH dilution remaining its stability?
Any advice on the following would be greatly appreciated
(1) cryopreservation solution, which is best for long term storage?
(2) removal of contaminating somatic cells, before or after freezing?
(3) cryo tubes or straws, which is best?
The last update of haploidization and its future perspectives was written by Drs Jan Tesarik and Carmen Mendoza in 2002 (Somatic cell haploidization: an update, Reproductive BioMedicine Online, 2002, 6(1):60–65) . Is there any new update or report of live births from this approach?
How can you predict and prevent infertile women who undergo IVF of having ovarian hyperstimulation syndrome after IVF?
I'm planning to perform epigenetic study on offspring born following Assisted Reproductive Technologies. I do not have an extensive experience on mouse model and I know that some strains are not so good for this kind of study. thus, which one should I use?
Thank you for your suggestion.
This is a cross match test to determine the presence of anti- lymphocyte antibody in female partners suffering from immune mediated infertility, Like that developed by Dr Alan Beer and currently offered in his Lab and Rosalind Franklin Lab, Chicago. Would also be used in transplantation studies
How Acridine Orange stain is used in semen evaluation?
I want to know procedure and name of stain (Source of procurement-company name) used to detect DNA integrity in Bovine spermatozoa.
I am injecting rRNA transcribed by mMESSAGE mMACHINE T3 Transcription Kit into Xenopus oocyte for 2 electrode recording. I am injecting 1ul per egg. What concentration is can be a start for this type of injection?
During artificial Insemination in Honeybee queens, someone uses chloroform to help endophallus eversion process in drones. Does anybody know if there is any paper concerning chloroform affecting sperm vitality (in any species.. not honeybee only)?
I am interested on legal and ethical aspects of human assisted reproduction in different cultures. Your opinion about the most questionable aspects is also welcome!
I want to develop fluorescent assays to evaluate the membrane integrity, capacitation status and acrosome reaction in bull and stallion semen. So, what are the possible results of this assay, and what sperm populations can I obtain after this analysis?
Due to assisted reproductive technologies and hormonal therapy there is higher risk of thromboembolism especially in cases with complicated course (for example with ovarian hyperstimulation syndrome)
I am developing a robotic system for cell injection that could be applied for ICSI. I would like to choose an IT platform and a language for programming. What kind of software is suitable for real time programming and control of different hardware devices as piezo-actuators, digital camera, microscope, micro-pumps. There are robotic toolbox, modules for image processing and many other modules for automation and control of robotic systems in real time in Labview and Matlab. I am not sure which language is better. In Matlab I can buy a specific hardware for control in opposite of Matlab, par example. The other possibility is to use linux OS, Qt4 programming language and OpenCV vision library as open source technology. The last option is to use Microsoft Visual Studio as a programming environment and selecting any programming language as C++ or C#. Could you please give me advice on what kind of a programming technology is best suited for development of software applications in real time? Thanks.
I treated the mature cumulus-free oocytes with pronase 0.3% (w/v in PBS), the zona pellucida was digested within 60-90 seconds however when the oocytes were first electrically activated (parthenogenesis) the digestion time became more longer (120-180 seconds or even more). Is there any explanation?
I am developing microfluidic devices for immobilization of biological cells. It is necessary to create hydrophilic surfaces. I saw in the literature that there are many plasma cleaning equipment that is very expensive. I would like to ask do you know any technology to create hydrophilic surfaces with home made cheaper equipment?
I have a percoll solution from sigma, and I want to prepare 80% 60% 40% and 20% percoll solutions to obtain pure sperm. How to prepare it and what is the buffer or other solution to be used for its preparation?
I would like to know which genes are considered the best progesterone marker in endometrial cell line. If I treat the cells with progesterone, an expression in which gene or protein is the best to proof the treatment.
Implantation of bone marrow stem cells in the testes is a hope for the treatment of males with primary testicular failure in humans. If this technique proves successful it will transform the treatment of male infertility. Basic research have shown the success of this technique in mouses.
I have a young patient, 31 years with amenorrhoea and very high LH and FSH. She wants to conceive. She bled when I challenged her with progesterone.
The principal idea of this technique is to have more GM-CSF and other growth factors, but the critical thing is how to know the concentrations precisely to avoid any contamination, alteration or apoptosis? How can we evaluate that? Any suggestions?
I mean does someone think that this cut off is the same for poor responders, normal responders and high responders?