Questions related to Aquatic Ecotoxicology
Currently, microplastics (MPs) occurence researches in coastal and marine animals are performed in huge amounts, but their results concerning MPs abundances are not always given with the same unity.
- From what I can tell from studies I read, most provide data using MPs/individual. There are some studies that sample in pools, and then, after ending samplings, calculate the MPs/individual data. It would be an issue if we compare MPs/individual data of two different studies: one that sampled in each individual and another that sampled in pools?
- Should a specific study provide just the MPs/individual data? I think this study it would be sort of incomplete, considering that this data would not totally reflect the abundance of animals with different sizes, weights, and possibly ages, etc.
- What is the best approach between other unities, such as MPs/g of the whole sample, MPs/g of their dry or wet weight? That are some formulas to convert samples weight for dry and/or wet. This is applicable for MPs?
I know much of that depends on the study's biological samples, and objectives, but I would enjoy reading researchers opinion about it.
In many conservatories around the world, as well as in many organic-farms, insecticidal soaps (potassium salts of fatty acids) are widely used to combat aphids, mealybugs, mites etc. They are considered safe to mammalians and are prioritized instead of chemicals.
Very little to no information can actually be found whether the soaps may be toxic to amphibians. Can anyone help us on this matter? An eductaed guess would tell me that the thin film created by the sopa on aquatic enviroment as well as, presumably, on the skin of the amphibians would cause significan damage.
Hi everybody. I am facing an issue about reproduction data analysis in chronic toxicity test with C.dubia. US EPA Method 1002.0 ( https://www.epa.gov/sites/production/files/2015-08/documents/short-term-chronic-freshwater-wet-manual_2002.pdf) instructions tell:
" The response used in the statistical analysis is the number of young produced per adult female, which is determined by taking the total number of young produced until either the time of death of the adult or the end of the experiment, whichever comes first. An animal that dies before producing young, if it has not been identified as a male, would be included in the analysis with zero entered as the number of young produced "
Including dead animals in the count gives me back too high standard deviation values which don't fit well in my analysis. I would like to know if alternatives are available: feel free to link me to other papers, works, methods etc.. which could help. Thanks a lot to anyone will spend time to help me.
At what point (concentration) is sulphate lethal to phytoplankton?
Any relationship between sulphate concentration and algal growth and development?
How can one contruct an experiment to determine the effect of sulphate concentration on phytoplankton?
I would much appreciate if you could explain or suggest good research papers regarding the effects of ocean acidification on the chemical composition of the valves of mainly Mytilidae and Pectinidae species and damage, growth, development or biomarker assessment.
Thanks in advance
The purpose of this question is to bring researchers together to discuss and network the issue of plastic waste in the sea. Both large waste items and plastic particle waste the likes ingested by fish and subsequently transferred through the food chain.
What research are you working or on or what suggestions do you have for scientifically removing the plastic both large and particle from our Oceans. Please share your thoughts.
Obviously, there is a large political movement on this subject at the moment and suggestions like, could we all own 1 bottle and have drinks dispensed rather than distributed in bottles. But what are peoples scientific thoughts / projects around the removal of plastic from the sea, rather than root cause.
Different groups favor different methods; I would greatly appreciate more input on the debate. Assume all bottle types are cleaned thoroughly and samples are being analyzed in the lab for Methylmercuy (MeHg), Total mercury (THg), both filtered and/or unfiltered. Other considerations: remote work, trace-level contamination, freezing vs. acidification.
I am a Masters Student of Ecotoxicology at University of Koblenz-Landau Germany. I am entirely new in this field and gradually developing interest in aquatic ecotoxicology. I will remain grateful to receive materials that will help me develop in this area of interest. Thanks.
The pufferfish also referred to as fugufish, blowfish or globefish is said to be the second most poisonous vertebrae on earth next to the tiny golden poison frog from Colombia. The toxin responsible for the pufferfish's deadly character is tetrodotoxin.
Two members of a family have died in Ghana today because of consuming this fish. What do you know about this fish and it's toxic element? How do we intensify public health education? Kindly share your valuable views. Thanks in advance.
There is a directive in Ghana by the Ministry of Fisheries and Aquaculture Department to ban all imports on ornamental fishes and Tilapia species including gametes-eggs and milts from now to the close of the year as an immediate response to halt the spread of the virus which is said to be prevalent in Africa, South America and Asia, especially in farmed Tilapia. This is a blow against the global Tilapia industry.
Kindly share your views on the new virus, ways of preventing it and other references. Thanks in advance. Best regards
I've got concentration of Cu2+, H+, and CuOH in water solution (in Visual MInteQ). Is complex BL-CuOH created from free ions like [Cu] and [OH], or does CuOH+ react with BL?
If I know concentration of free ions of Cu nad CuOH+ ions - which of them should I use to calculate concentration of BL-CuOH.
Metal nanoparticles are showing promise in tackling different problems in different domains of environment. How they can be used in aquatic ecosystems? What are different likely interactions? What may the possible toxic effects on different ecosystem components (planktons, bacteria, macrophytes and fish)? How can we track the ecotoxicological linkages? How could we manage metal nanoparticles for positive (beneficial) uses and avert the adverse/untoward consequences?
RG friends and researchers you all are welcome to participate and help to promote a sustained brainstorming on pros and cons of apllyting metal nanoparticles in aquatic systems like, ponds, lakes, rivers, etc.
Toxicity values for Ni on Coastal and marine organisms especially diatoms and copepods from coastal waters of India through toxicological bioassays
There always have been disturbances occurring naturally in these types of ecosystems, but it would be good to know which is being more affected by human interference.
I m looking for some literature that describes the method to measure the trophic level transfer efficiency in marine ecosystems directly on the field in-situ.
Bioturbation is the soil/sediment reworking by animals. It has a definite role in ecosystem engineering too by modifying and modulating different physicochemibal transformations and biological/microbiological interactions. Toxicants' transfers may be affected and ecotoxic effects may be modified. Can bioturbation be manipulated in reducing toxic effects of some toxicants and how?
I have found ucc value for Fe (from Rudnick & Gao 2004) is 4.09 wt%. but how can I convert it into mg/kg unit?
advance thanks for response and precious time.
I am currently working on the bioaccumulation of nickel in Sargassum polycystum, but I can't seem to find the level limit of nickel in said species. Anyone who knows the level limit in accordance to WHO and NAOCC?
I am interested in marine fish (muscle vs whole body) relationships.
Two-way ANOVA on a dataset (codes and results given below) showed no interaction. However, the post hoc output from lsmean package showed a compact letter display which sounds like there is an interaction. The compact letter display for "treatment 2" at "week1" is "c" whereas it is "ab" at week2 and week3, respectively. Does this show an interaction (the effect of treatment changes with change in week)or am I misinterpreting the result? The codes given below.
read.table(textConnection("time treatment effect
week1 1 664
week1 1 617
week1 1 647
week1 2 732
week1 2 819
week1 2 843
week1 3 850
week1 3 670
week1 3 722
week2 1 561
week2 1 581
week2 1 586
week2 2 597
week2 2 669
week2 2 654
week2 3 747
week2 3 708
week2 3 705
week3 1 630
week3 1 630
week3 1 664
week3 2 576
week3 2 666
week3 2 716
week3 3 776
week3 3 773
week3 3 726
tk <- lsmeans(m1, pairwise ~ treatment*time,adjust="tukey",sort=F)
I find a lot of answers about aquatic toxicity on plants, but none on aquatic toxicity of plants (except one or two articles about plants used for fish poisoning, in fishing). My keywords are not very useful to distinguish these two subjects! So, I am looking for some general or review documents on the subject, in particular, if possible, about toxicity of plant extracts.
There are many research papers published on Potassium permanganate (KMnO4) bleaching which state that it is sustainable process to do bleaching with potassium permanganate.
I agree with conducted research regarding effectiveness and results obtained through use of potassium permanganate, but I have come to know recent news that KMnO4 is restricted in many countries and it is non-biodegradable at all.
CHT Bezema has introduced alternative of KMnO4 which is 99% biodegradable, you may visit following link to know about their product.
As per CHT report, Why KMnO4 is restricted?
Manganese is a heavy metal and not biodegradable. Potassium permanganate (KMnO4) belongs to the substances which are particularly dangerous to the environment with high fish toxicity. In many countries there are strict regulations or even an obligation to provide evidence to avoid any misuse of KMnO4.
Please share your valuable information and thoughts.
For fish species (e.g. Anabas testudineus) that can survive in stagnant water and certain levels of heavy metal contamination (of nickel and copper), could they have certain specializations that allow them to continue to live in these waters? Also, would the contaminants remain in their flesh (and prevent them from being viable from human consumption)?
i want find out the relationship between the ones that was found in oily polluted areas with the one of thee protozoan that i have in our lab that i used for bio-remediation studies.
Anyone tell me more about the chemical pathways of bioaccumulation of METALS in aquatic life? Interested in chemistry than toxic effects.
I am interested in how certain plankton or bivalves accumulate metals in their body, I am trying to understand the organometallic chemistry as well as the kinetics.
E.g.Wang et al 2008
or Emmanuel et al 2011 (on bacterial accumulation).
I am having trouble locating a general but detailed description of the anatomy of Lumbriculus variegatus, in particular information concerning anatomy and conditions in digestive organs.
I just want to estimate the contribution of algal dry weight to particulate organic carbon dry weight. I have calculated the ash free dry weight and Chl a of an eutrophic lake. Can you please suggest me a relatively better ratio, so that I can determine/estimate algal dry weight?
Unable to find papers on automated video tracking of aquatic invertebrates in multiwell plates, I was hoping to find individuals here that have experience in the laboratory doing this or related automated video tracking research. If so, can you give me a brief synopsis of your research questions/topic and what software you are using. Thank you, Lou
Louis Macovsky, DVM, MS
Dear friends, Hello, I have enjoyed the conversations very much and first of all I want to thank you for sharing. I am a PhD student in marine biology and decided to work in the subject of Microplastic ingestion by fishes in Southern Caspian Sea. At first part of my work, we are going to examine fish intestines to find any anthropocentric object and in second part, feed juvenile fish with mixtures of food and 0.5 to 5 micron Microplastic (MP) to find their possible cellular movements and effects on cell mechanisms of entrocytes. To find out, I want to use Transmission Electron Microscopy Technique to see weather MP is found in entrocytes of intestine or not and is there any effect on ultrastructure of entrocytes. I have written a proposal and presented it but some experts had doubt about efficiency and practical possibility of this method. I have used the Technique for normal tissues of different fish species but some people think in the EM images, MP will show up as empty spaces and they may be confused with other objects or artifacts. Therefore, I appreciate if you help me in this case. I need to know what you think about it. Happy 2016 and hope you have a nice holiday. Cheers, Zahra
We have tried to identify the influence of riparian vegetation on water quality and macroinvertebrates diversity. We have tried to correlate the vegetation type with the different variables however this doesn't answer the question. We made CCA using the vegetation species, water and environmental variables along with the sampling sites. We have observed different water quality characteristics along with the vegetation type.
What better statistical analyses do you recommend?
I' d like to calculate lethal concentration (LC50) or effect concentration (EC50) in our study with daphnids. The US EPA suggest to derive values using both the probit method and the trimmed Spearman-Karber method. Do you know if there is a (free) software or an R code to perform these analyses?
Can someone please recommend a software analysis tool that use water quality parameters (EC, SAR, pH, salt concentrations, etc) to evaluate quality of irrigation water and potential effect on soil health?
When I prepared a serial dilution for measuring lethal concentration of aluminum in fish, the water pH in higher concentrations will be reduced dramatically (i.e. from 7.2-7.4 in control reaches 5.4 for example).
NOTE: Aluminum chloride was used for preparing the stock solution.
Is there anyway to put water pH in a stable range during acute toxicity test of fish?
I have used utensil sponges as the filter in wastewater treatment. But now I wonder how to extract effectively antibiotic resistance gene from these sponge. Could you suggest me some ideas?
Does anyone known some record of these taxa in karstic water bodies?
There are some report of these taxa in freshwater or hyposaline water?
Thanks and greetings.
And what types of chemicals are used in the production process of silicon carbide?
Toxicity in aquatic organisms
How can calcalute the LC50 and ED50 of toxic metals exposed to aquatic organisms ?
There is a lot of literature on embryo and larval toxicity but there seems to be almost nothing on adults except for:
Bielmyer GK, Brix KV, Capo TR, Grosell M (2005) The effects of metals on embryo-larval and adult life stages of the sea urchin, Diadema antillarum. Aquatic Toxicology 74:254–263
I am especially looking for studies providing LC or EC50s
I am searching for an organism that is also easy to breed in the aquarium and reproduces relatively quickly (say up to 2 or 3 months is ok). I am open to both salt- and freshwater organisms!
I'm looking for articles with the top result of kindly Bivalve for bioindicators, biomarkers in pollution biomonitoring from your country. Any suggestions, please?
I already tried universal primers (Folmer et al., 1994) and others like COI-E and AnnCOIF used in published papers, with different temperature programs but without results. Does anyone has a PCR protocol that already used and worked sucessfully for these annelids?
I'm currently working on an assignment on the degradation of microplastics in the marine environment, I have found a good breadth of background knowledge and literature on the degradation of plastic debris to microplastics but i'm having trouble finding literature on the further degradation microplastics to nanoplastics and thir impacts etc. Any help would be greatly appreciated!
If we know the wavelenght absorption of the compounds that we would identify, it is possible to work with other types of detectors i.e., absorbance detectors?
Moreover, it is possible to work on metabolism with fluorescence detectors? It is possible to use labelled fluorescent precursors?
DDT and its metabolites are well known and while there is data available on the effects on frogs and other amphibians, I am struggling to find a paper describing the metabolic pathways followed when amphibians are exposed to DDT or other persistent pesticides.
I am want to using a population level endpoint to assessment the toxocity effect of EDs (e.g. EE2, NP ) to fish, daphina and algae. How can I use population level endpoint to deriving PNEC for these chemical when the toxicity data less than 5 or less?
Rope mussel aquaculture has reached its maximum potential of the west coast of Ireland but as a consequence does the reduction in phytoplankton species have an impact on wild fish (particularly salmonids) species down the food chain?
I am exploring technology that can result in a low cost, onsite assessment of aquatic invertebrate behavior (movement) as an endpoint for ecological risk assessment. If statistical analysis can confirm a difference between movements of individual aquatic invertebrates upstream, in, and below stream of point discharge, could this be of use for phase 1 or other studies of risk assessment? Thanks, Lou
Microbial communities can be affected by Cadmium, Mercury and others heavy metal. How selective pressure occur in this kind of habitat?
there are several conversation on restoration of a natural habitat; i wish to know what are the various ways to restore an flowing aquatic polluted system in to its natural state
I'm reviewing information on the issue of low-cost methods in ecotoxicology, and I have some studies on it, such us Mills CL et al. 2006 Development of a new low cost high sensitivity system for behavioural ecotoxicity testing Aquatic Toxicology 77: 197-201 I would like to know other studies on it.
there are several advancements in the analysis and monitoring of freshwater bodies in all over world; but i need to know the current advancements in the same. like modelling, analysis, prediction, statistical applications etc., can anyone help in this regard
We are in the process of building a mesocosm facility (freshwater). There is a lot of input from literature on dimensions of ponds etc. Yet recently we heard about the existence of a guidance document (or in development?). Does anyone know more?
I'm looking for a glasware (or similar) set up that allows cultivating 2 different cultures, whose growth medium is interconnected (eg membrane filter) which allows permeability of dissolved compunds from one flask to the other, but maintains both cultures separated. Any idea if this kind of set up is available and where?
We find that each pipette drop of brine shrimp in water deposits a highly variable amount. Does anyone use a tool or technique in their work that could potentially be co-opted to dispense a small predetermined number or weight of tiny brine shrimp?
Trying to make culturing more easier and wondering about advantages and disadvantages of dry algae as a food for Daphnia magna
I'm looking for a link between ETC activity measurement and DEB model parameters...