Applied Microbiology - Science topic

Air is made up of enormous number of microorganisms mainly fungi and bacteria spore. Their estimation is important as an indication of cleanliness of any particular environment. The present study was carried out to assess microorganisms in indoor air of Applied Microbiology laboratory of Nnamdi Azikiwe University, Awka. Prepared plates of Sabouraud...

Air is made up of enormous number of microorganisms mainly fungi and bacteria spore. Their estimation is important as an indication of cleanliness of any particular environment. The present study was carried out to assess microorganisms in indoor air of Applied Microbiology laboratory of NnamdiAzikiwe University, Awka. Prepared plates of Sabouraud...

Mapping of research has become important across the world and any new technology requires a new institutional framework for mapping appropriate outcomes of research. It involves analyzing linkages between various actors, stakeholders, agencies, and institutions to map potential research domains. Over the years, Biotechnology Applied Microbiology ha...

As per the New Syllabus of Indian Nursing Council 2021 for B.Sc Nursing Students. This book also helps the GNM, ANM, Allied Health, and Medical Sciences Students.

Air is made up of enormous number of microorganisms mainly fungi and bacteria spore. Their estimation is important as an indication of cleanliness of any particular environment. The present study was carried out to assess microorganisms in indoor air of Applied Microbiology laboratory of Nnamdi Azikiwe University, Awka. Prepared plates of Sabouraud...

This work explores the degradation of xenobiotic compounds in aerobic and anaerobic batch reactors. Different inoculums were spiked with nine emerging contaminants at nominal concentrations ranging between 1 to 2 mg/L (ibuprofen, diclofenac, naproxen, acesulfame, sucralose, aspartame, cyclamate, linear alkylbenzene sulfonates, and secondary alkyl s...

Air is made up of enormous number of microorganisms mainly fungi and bacteria spore. Their estimation is important as an indication of cleanliness of any particular environment. The present study was carried out to assess microorganisms in indoor air of Applied Microbiology laboratory of Nnamdi Azikiwe University, Awka. Prepared plates of Sabouraud...

Heavy metals are elements that can have toxic effects even at low concentrations. Heavy metal pollution is one of the most concerning issues at the global level because of the health risks it causes and its negative effects on the ecosystem. Many studies have been carried out in the past years to reduce heavy metal emissions, pollution, and their e...

I was invited to present my work as key note speaker in “International Conference and Expo on Applied Microbiology” held Online during June 17-18, 2022

Harmful Microcystis blooms (HMBs) and microcystins (MCs) that are produced by Microcystis seriously threaten water ecosystems and human health. This study demonstrates an eco-friendly strategy for simultaneous removal of MCs and HMBs by adopting unique hyperoxic graphene oxides (HGOs) as carrier and pure microcystinase A (PMlrA) as connecting bridg...

The Main Aspects of End-to-End Standardisation of Herbal Drugs, Herbal Drug Preparations, and Herbal Medicinal Products of Eucalyptus viminalis 1 All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR), 7/1 Grin St., Moscow 117216, Russian Federation 2 Closed Joint Stock Company VIFITEH, State Research Center for Applied...

Purpose Wood decay fungi are the only forms of life capable of degrading wood to its initial constituents, greatly contributing to the soil ecosystem. This study summarizes the current research status and development characteristics of global wood decay fungi research, in order to better understand their role in soils. Methods A bibliometric analy...

In patients with acute respiratory failure, mechanical ventilation through an endotracheal tube (ET) may be required to correct hypoxemia and hypercarbia. However, biofilm formation on these ETs is a risk factor for infections in intubated patients, as the ET can act as a reservoir of microorganisms that can cause infections in the lungs. As severe...

Microalgae-based technology is an effective and environmentally friendly method for antibiotics-contaminated wastewater treatment. To assess the tolerance and removal ability of Chlorella sorokiniana to ciprofloxacin (CIP), this study comprehensively revealed the responses of C. sorokiniana to CIP exposure and its degradation processes through phys...

RESUMEN Apoyados en la microbiología aplicada, se buscará un microorganismo que pueda ayudar a solucionar el problema que actualmente vive el suelo agrícola. La necesidad que tienen las plantas respecto al elemento fósforo para expresar todas sus propiedades y genética, hace que los productores se vean en la obligación de adicionarlo al suelo, cree...

BOOK TITLE: INSIGHTS IN INDUSTRIAL MICROBIOLOGY Abstract This work was designed to give insights on the various key topics in industrial microbiology. The topics discussed include screening for microbes of industrial importance and industrial products, detection and assay of fermentation products, aspect of the biology of industrial culture techn...

This book covers recent advances and future trends in yeast synthetic biology, providing readers with an overview of computational and engineering tools, and giving insight on important applications. Yeasts are one of the most attractive microbial cell factories for the production of a wide range of valuable products, including pharmaceuticals, nut...

In the COVID-19 pandemic, the biotechnology sector has proved as boon to combat with certain circumstances, where most contribution of biotechnology sector has been found in chemotherapy and vaccination programme. Thereafter, the laymen too understood the scope and importance of biotechnology. The term biotechnology was coined by a Hungarian engine...

The first international seminar on pollution, health, environment and bio-monitoring, will be organized by the Department of Science and Nature and Life of the University of August 20, 1955-Skikda online (web meeting); this is in projection of the effect of Covid-19 pandemic. Public health, the appearance or disappearance of certain living species,...

In 2016, the United States Department of Agriculture (USDA) Food Safety and Inspection Service (FSIS) established guidelines which modified the Buffered Peptone Water (BPW) rinsate material to include additional compounds that would better neutralize residual processing aids and allow for better recovery of sublethal injured Salmonella spp. cells....

The spike glycoprotein of SARS-CoV-2 engages with human angiotensin-converting enzyme 2 (ACE2) to facilitate infection. Here, we describe an alpaca-derived heavy chain antibody fragment (VHH), saRBD-1, that disrupts this interaction by competitively binding to the spike protein receptor-binding domain. We further generated an engineered bivalent na...

The major transmission route for SARS-CoV-2 is airborne. However, previous studies could not elucidate the contribution between large droplets and aerosol transmission of SARS-CoV-2 and its variants. Here, we designed and validated an optimized transmission caging setup, which allows for the assessment of aerosol transmission efficiency at various...

Avant-propos Les microorganismes sont une composante essentielle de tout écosystème. Ils occupent des micro-habitats spécifiques convenant à leurs besoins. Dans ces micro-habitats, les microorganismes ont établi différents types de relations entre eux et avec d'autres organismes : neutres, symbiotiques, pathogènes et antagonistes. De par ces relati...

Ce rapport détaille les activités menées par les membres de l'URMAPha au titre de l'année 2021.

Atlantic salmon aquaculture is expanding, and with it, the need to find suitable replacements for conventional protein sources used in formulated feeds. Torula yeast ( Cyberlindnera jadinii ), has been identified as a promising alternative protein for feed and can be sustainably cultivated on lignocellulosic biomasses. The present study investigate...

Metarhizium anisopliae is a promising alternative to chemical pesticides against pine wilt disease caused by Bursaphelenchus xylophilus . Herein, we investigated the efficacy of modified atmosphere packaging (MAP) to prolong the shelf-life of the M. anisopliae conidia. The effects of various conditions on its stability were also examined. M. anisop...

Pseudomonas aeruginosa is an opportunistic bacterium causing several health problems and having many virulence factors like biofilm formation on different surfaces. There is a significant need to develop new antimicrobials due to the spreading resistance to the commonly used antibiotics, partly attributed to biofilm formation. Consequently, this st...

In recent years, white rot fungi (WRFs) have received tremendous attention as a biotechnological tool for environmental pollution control. In order to systematically and comprehensively describe the progress, trends, and hotspots of WRF biotechnology in the field of environmental pollution control, the 3967 related publications from 2003 to 2020 we...

The aims of this study were to synthesize highly positively charged chitosan nanoparticles (Ch-Np) using the electrospraying technique, and to test their antimicrobial activity against endodontic pathogens, and cytotoxicity against fibroblast cells. Ch-Np were synthesized from low molecular weight chitosan (LMW-Ch) using the electrospraying techniq...

The early-life modifications of intestinal microbiota may impact children's subsequent emotional and cognitive development. Studies show that some bacteria species in gut microbiota, and the lack of others, may play a key role in autism spectrum disorders (ASD) development. Fecal samples were obtained from three groups of children: 16 healthy, 24 w...

COVID-19 has restricted singing in communal worship. We sought to understand variations in droplet transmission and the impact of wearing face masks. Using rapid laser planar imaging, we measured droplets while participants exhaled, said ‘hello’ or ‘snake’, sang a note or ‘Happy Birthday’, with and without surgical face masks. We measured mean velo...

The role of probiotics in mitigating constipation, gut immunity, and gut microbiota has not been well studied. We aimed to evaluate the effects of probiotics on loperamide (LP)-induced constipation in Sprague–Dawley rats. Altogether, 150 male Sprague–Dawley rats (age 8 weeks) were used in the experiments following a 12-day acclimatisation period an...

The main objectives of this study were to isolate bacteria from soil chronically contaminated with polycyclic aromatic hydrocarbons (PAHs), develop an autochthonous microbial consortium, and evaluate its ability to degrade PAHs in their native contaminated soil. Strains with the best bioremediation potential were selected during the multi-stage iso...

Cruciferous vegetables are rich sources of glucosinolates (GSLs). GSLs are degraded into isothiocyanates, which are potent anticarcinogens, by human gut bacteria. However, the mechanisms and enzymes involved in gut bacteria-mediated GSL metabolism are currently unclear. This study aimed to elucidate the enzymes involved in GSL metabolism in lactic...

The demand for n-3 long-chain polyunsaturated fatty acids (n-3LC-PUFAs), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), will exceed their supply in the near future, and a sustainable source of n-3LC-PUFAs is needed. Thraustochytrids are marine protists characterized by anaerobic biosynthesis of DHA via polyunsaturated fatty aci...

Engineered living materials (ELMs) are a fast-growing area of research that combine approaches in synthetic biology and material science. Here, we engineer B. subtilis to become a living component of a silica material composed of self-assembling protein scaffolds for functionalization and cross-linking of cells. B. subtilis is engineered to display...

Optimisation of cultivation conditions in the industrial production of probiotics is crucial to reach a high-quality product with retained probiotic functionality. Flow cytometry-based descriptors of bacterial morphology may be used as markers to estimate physiological fitness during cultivation, and can be applied for online monitoring to avoid su...

Murine norovirus (MNV) was used as a surrogate for human viral pathogens (e.g., norovirus) to determine if toilet flushing resulted in the aerosolization of virus. A flushometer type toilet was seeded with a viral solution of 10⁵ and 10⁶ PFU mL⁻¹ of MNV and then flushed. Upon flushing, two bioaerosol samplers were activated to collect aerosolized M...

The objective was to evaluate the effect of dietary Bacillus altitudinis spore supplementation during day (D)0–28 post-weaning (PW) and/or D29–56 PW compared with antibiotic and zinc oxide (AB + ZnO) supplementation on pig growth and gut microbiota. Eighty piglets were selected at weaning and randomly assigned to one of five dietary treatments: (1)...

Accumulating evidence indicates that gut microbiota may regulate sex-hormone levels in the host, with effects on reproductive health. Very little is known about the development of intestinal microbiota during puberty in humans. To assess the connection between pubertal timing and fecal microbiota, and to assess how fecal microbiota develop during p...

Obesity and obesity-related metabolic disorders are linked to the intestinal microbiome. However, the causality of changes in the microbiome–host interaction affecting energy metabolism remains controversial. Here, we show the microbiome-derived metabolite δ-valerobetaine (VB) is a diet-dependent obesogen that is increased with phenotypic obesity a...

Gene clusters rich in carbohydrate-active enzymes within Flavobacteriia genera provide a competitiveness for their hosts to degrade diatom-derived polysaccharides. One such widely distributed polysaccharide is glucuronomannan, a main cell wall component of diatoms. A conserved gene cluster putatively degrading glucuronomannan was found previously a...

Managing postprandial glycemic response, or the increase in blood sugar following a meal, is a crucial component to maintaining healthy blood sugar in patients with diabetes. To test whether oral probiotics can impact postprandial glycemic response, E. coli Nissle 1917 (EcN) was evaluated in an oral glucose tolerance test. Oral gavage of EcN concur...

Sessile microorganisms were described as early as the seventeenth century. However, the term biofilm arose only in the 1960s in wastewater treatment research and was adopted later in marine fouling and in medical and dental microbiology. The sessile mode of microbial life was gradually recognized to be predominant on Earth, and the term biofilm bec...

Chemical decontamination during processing is used in many countries to mitigate the Campylobacter load on chicken meat. Chlorine is a commonly used sanitizer in poultry processing to limit foodborne bacterial pathogens but its efficacy is limited by high bacterial loads and organic material. Acidified sodium chlorite (ASC) is a potential alternati...

Diet is a key factor influencing gut microbiota (GM) composition and functions, which in turn affect host health. Among dietary regimens, time-restricted (TR) feeding has been associated to numerous health benefits. The impact of TR feeding on the GM composition has been mostly explored by means of metagenomic sequencing. To date, however, little i...

We designed two probiotic treatments to control chytridiomycosis caused by Batrachochytrium dendrobatidis (Bd) on infected Panamanian golden frogs (Atelopus zeteki), a species that is thought to be extinct in the wild due to Bd. The first approach disrupted the existing skin microbe community with antibiotics then exposed the frogs to a core golden...

Biofilms have several characteristics that ensure their survival in a range of adverse environmental conditions, including high cell numbers, close cell proximity to allow easy genetic exchange (e.g., for resistance genes), cell communication and protection through the production of an exopolysaccharide matrix. Together, these characteristics make...

In this work, we hypothesized that shifts in the food microbiome can be used as an indicator of unexpected contaminants or environmental changes. To test this hypothesis, we sequenced the total RNA of 31 high protein powder (HPP) samples of poultry meal pet food ingredients. We developed a microbiome analysis pipeline employing a key eukaryotic mat...

Maintaining functional stability of microbial electrolysis cell (MEC) treating wastewater depends on maintaining functional redundancy of efficient electroactive bacteria (EAB) on the anode biofilm. Therefore, investigating whether efficient EAB competing for the same resources (electron donor and acceptor) co-exist at the anode biofilm is key for...

This year, 2022, Microbial Biotechnology (MBT) celebrates its 15th birthday. In journal terms, 15 is adulthood. It has been a privilege to develop the idea, launch the journal, nurture it in its infancy and stimulate and support it during its adolescence and early adulthood. The success of MBT – its growth over the last 4 years averaging > 30%, and...

High cell density cultivation necessitates cell division and biomass formation, the mechanisms of which remain poorly understood, especially from the cellular energetics perspective. Specifically, the sensing of energy abundance and the channelling of nutritional energy into biomass formation and cell maintenance remains enigmatic at the sensory, e...

Raman microspectroscopy offers microbiologists a rapid and non-destructive technique to assess the chemical composition of individual live microorganisms in near real time. In this Primer, we outline the methodology and potential for its application to microbiology. We describe the technical aspects of Raman analyses and practical approaches to app...

Microbial populations are a promising model for achieving microbial cooperation to produce valuable chemicals. However, regulating the phenotypic structure of microbial populations remains challenging. In this study, a programmed lysis system (PLS) is developed to reprogram microbial cooperation to enhance chemical production. First, a colicin M -b...

As antibiotic resistance is being a threat to public health worldwide, bacteriophages are re-highlighted as alternative antimicrobials to fight with pathogens. Various wild-type phages isolated from diverse sources have been tested, but potential mutant phages generated by genome engineering or random mutagenesis are drawing increasing attention. H...

Yaks and Tibetan sheep are important and renowned livestock of the Qinghai-Tibetan Plateau (QTP). Both host genetics and environmental factors can shape the composition of gut microbiota, however, there is still no consensus on which is the more dominant factor. To investigate the influence of hosts and seasons on the gut microbiome diversity compo...