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Hello,
I would like to know how much long can I conserve serum samples in - 20°C for biochemical analysis : lipid profile (cholesterol, triglyceride...), protein profile (albumin, total protein...), enzymatic (ASAT, ALAT...), glucose .
Thank you in advance
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Answer: Prior to shipment or for a maximum of 48 hours, serum should be kept between 4 and 8°C. 7 days. Serum samples held for longer periods of time should be frozen at or below 20°C and sent to the testing facility on frozen ice packs.
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Is there any publication that contains this data?
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Hello!
Ascites in broiler chickens results from an imbalance between the amount of oxygen available and the amount needed to maintain rapid growth rates and high feeding efficiency. Ascites is influenced and/or caused by variables such growth rate, altitude (hypoxia), and ambient temperature because of the link to oxygen requirement.
Toxins from plants like Crotalaria or aflatoxin may harm the liver in chicken. Obstructive cholangiohepatitis, which is brought on by an infection with Clostridium perfringens, is the most typical cause of liver damage and ascites in broiler chickens. Ascites can result from hepatic amyloidosis in breeders as well as meat-type ducks.
For a more detailed explanation, please refer to this article:
Hargis, B. M. (2022, August 4). Ascites syndrome in poultry - poultry. MSD Veterinary Manual. from https://www.msdvetmanual.com/poultry/miscellaneous-conditions-of-poultry/ascites-syndrome-in-poultry
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Dear Scholars/Scientists/Researchers,
What is the genus name of white leg shrimp? Some authors state species name as Litopenaeus vannamei and some as Penaeus vannamei? Are these two names of the same species?
Thank you for your answers.
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Whiteleg shrimp (Litopenaeus vannamei, synonym Penaeus vannamei), also known as Pacific white shrimp or King prawn, is a species of prawn of the eastern Pacific Ocean commonly caught or farmed for food.
Whiteleg shrimp - Wikipedia
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Hi,
I used the Latex template of JASB (Journal of Animal Science and Biotechnology) to submit a manuscript. The produce PDF using overleaf looks pretty well and the initial submission went well. For the revised version, the journal asked to upload the PDF and the latex files (e.g. .tex, .bib, .cst).
I realised that there were some discrepancies with the references and it took me a while to made some minor changes to the template but I finally succeeded to get it right (i.e., in some cases I needed to change the information of the .bib file, I needed to point to the vancouver.cst file in the \bibliographystyle command, and I edited the bmcart.csl file in order to use comas instead of semicolon between keywords of the abstract).
I compiled latex in overleaf and do not have any 'red' warnings and the PDF came out perfectly. But, I do get overfull and underfull warnings for hbox and vbox, for websites references says author is missing.
When I upload the files into the editorial system of the journal, the compilation does not work well and the built document does not really show my manuscript but errors.
Does anyone have recommendations/tips to get this submission using Latex right? Otherwise, it seems that I will need to make a word document, which would be a large waste of time.
Thank you in advance!
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What errors do you get from the journal compiler? I assume you've already perused the "Additional information for TeX/LaTeX users" here: https://jasbsci.biomedcentral.com/submission-guidelines/preparing-your-manuscript
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I need a list or site where I can easily find journals with no APC. The journals' scope should be Animal sciences, Biotechnology, Genetics and Molecular Biology.
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You have really put up a very important current issue as large number of existing highly reputed journals in the field of animal science have now been shifted to open access mode and so called paid journals. In addition, large numbers of new open access journals are emerging rapidly with APC. Its really challenging for low income and middle income countries scientists to find out the source of funding for publishing their high quality science in internationally reputed journals. No need to mention, but several highly reputed international journals are now charging in the name of making published article full PDF available online/download and to increase its accessibility/visibility globally.
However, scientists from major part of the world (except few countries where fund is allocated/available for research publications or the member of some or other organizations) are having similar issues that have resulted them to keep on searching appropriate highly reputed international journals (with high impact) that do not charge for publication (no publication charges) or do not have APC.
Coming to your point, although not all journals but still several journals in the field of animal science from major publishers like Elsevier, Springer nature, Wiley Online Library etc are accepting manuscript without any charges/APC.
Although I am not giving you the name of the journals herewith, you can go through these publishers website and I am sure you can find out several highly reputed internationals journals in the field of animal science for your research publications.
Go through the google search by adding "list of animal science journals with impact factor" .
You get a list then see the journals which are not showing open lock (indicative of open access, most of the open access journals are paid except few).
Search the journals that deals with the field of your research interest and see their impact factor. Once you are satisfied that they do not charge and having international reputation and indexing, available on major search engine like PubMed/PubMed Central etc., submit your piece of research article for publication.
There are several journals that give option of open choice: For examples, The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health.
Authors who choose to publish open access in Journal of Assisted Reproduction and Genetics are required to pay an article-processing charge.
Best
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This questionnaire is for dog owners. This is part of my disseration for animal science. I am looking to see if canines with anxiety are more at risk of becoming ill. The data will only be used for the purpose of this disseration. even if your dog does not have any anxiety please fill in as it may just prove anxiety has no effect on a dogs health.
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I'll be interested to see the results, with the proviso that owners are not always the best judge of their dogs! Also, "gender" should be "sex".
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semen cryobiology and poultry
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- The cylindrical-shaped head of cock spermatozoa and unique lipid composition causes plasma membrane damage during cryopreservation.
- Glycerol is the cryoprotectant used in cryopreservation and its large molecular weight causes low permeability. glycerol needs to be removed after cryopreservation and this is costly, difficult, and work-intensive.
- osmotic intolerance of chicken spermatozoa
- loss of lipid from membrane and cause damage to the membrane
- expensive to make more number of straw with very less quantity of semen
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Dear all.
This is a common practice to prefix Dr. After completing PhD in any discipline. May be science arts or commerce. This really creates confusion many times.
Peers plz suggest and if possible may something be done to eliminate this confusion . this is a personal query. nothing personal to anybody. Just enlight what you feel.
Responses awaited?
Regards
Partha
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The 'D' in PhD stands for Doctor so all PhDs can use the title of Doctor by the original latin usage going back many centuries. Thus academic PhDs are the real doctors by definition. The common usage of Doctor as a title in the medical profession has come about much more recently, but strangely surgeons insist on being called Mr, Ms, etc. Thus the real question is - should medical practitioners without a PhD (most of them) be entitled to be referred to as Doctor?
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An extensive systematic search resulted in an overview of all the animal victims from covid litter found worldwide, including entanglements, ingestions, and entrapments. Thanks to nature photographers, animal rescue centers, birdwatchers and litter pickers, we learned a great deal about just how harmful PPE products can be. Monkeys, fishes, hedgehogs, penguins, crabs, and many more animals are affected, even pets like cats and dogs.
Share your observations!
We started a database where anyone can share further observations of interactions between animals and PPE litter. As we only searched for articles in English and Dutch and found mainly cases from the UK, USA, and The Netherlands, we expect that there must be many more examples! Feel free to add your own observations or add new examples - from your local or national news, or social media - that are currently missing in the global overview. Let us know!
Find the overview at: www.covidlitter.com
Thanks.
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It affected badly.
Thank yu for this platform
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Hi everyone,
I am doing an allantoin assay in urine and all the protocols seem pretty vague. I am currently using this website which seems to be the most helpful:
It mentions that I use 15 mL tubes, but since I am boiling the samples should I use glass tubes with a cap to prevent escape? Or can I just use plastic tubes like the ones here:
Thank you!
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Nice question
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I’m new in the field of measuring heart rate and heart rate variability. I’m particularly interested in measuring heart rate in dogs and sheep in connection to stress. I would be very grateful for some advice regarding the equipment for monitoring the heart rate-what monitors do you use, what do I have to pay attention to (there are so many heart beat monitors on the market-which one is good and why…)?
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Polar is the best way to go. The chest belt is worth trying. I recommend contacting directly Polar, they have the longest experience with measuring ECG recordings.
It would be worth to try microcomputer-based devices (Raspberry Pi and similar ones). What you need is a microcomputer, wifi, and electrodes on a chest belt. It can cost in the order of tens of dollars.
Some colleagues from electronic faculty can put it together. 12 bits wide potential recordings exported into the CSV file will serve as a satisfactory input for any software evaluating ECG recordings.
When you got ECG, you can use complexity measures to follow the complexity of the ECGs. It gives much more data than HRV.
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Dear all.
During publishing an article what is the permissible range of plagiarism.?.
Many times we publish more than one articles from a sinhle study where the study and materials and methods are almost same. In biological science clubbing more parameters creates confussion amongst the readers and also to draw the conclusion of the finding. And in most of the journals there is a set word limit. So mostly we cant place the whole study in one article. Thus we split into many depending the extent of study. But there cones the plagiarism while publishing. As most of the methods like feed preparation feeding procedures etc so automatically it seems to be high familiarity index but originally the study is novel. Hence please guide the researchers in this regards how this can be eliminated. .. and a request to editors can similarity index be considered for intro results and discussion only. Even references may give rise to higher similarity index.
Please guide us.
Thank you
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Indeed, Prof. Deepak K. Goyal gave a complete answer.
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I've measured stable C and N isotopes in sequential segments of dingo vibrissae, and hope to determine whether there are any seasonal patterns in their diet. To really do this analysis justice I need to know the growth rates of vibrissae (preferably mystacial). Data from a Canis sp. would be best but another genus will do if they're not available. Thanks for your time.
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I want to install GPS trackers on hare (Lepus timidus).
What I need is probably an GPS/GSM logger, weight approx. <50 g
·        Is there someone here, having experience in this kinds of work?
·        How works the GPS-collar – What is the price - How reliable are they?
·        Is it some company/web page you can recommend?
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HI, I am interested in experience regarding brown hare (Lepus europaeus) tracking devices.
When is the best time to set up a tracking device for brown hare that need to be released from the rearing farm in to the hunting ground.
It is clear before we release him, but there is a fear that he will under huge stress during fastening on. Is there any safe way to do this?
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Hello everyone
Sometimes interpreting biological terms/pathways related to a particular phenotype might be very difficult and confusing. I have read many papers which only discussed a few selections of pathways related to their objectives of study and simply ignored the rest of pathways and terms (buried in supplementary materials!). This is an issue which needs an urgent solution. In Animal Science, we work with traits e.g. milk production or birth weight which are complex traits and multiple genes involved in the whole process. Although through GWAS studies we know most of genes involved in a complex trait. To my knowledge the "gap" is: there is no direct biological pathway (for example in Cytoscape or David) related to milk production.
I know "mammary gland development" or "cell-cell junctions" or other known terms, together are related to milk production but they need to be altogether in one term. This was only one example among many other important traits.
I would like to hear other peoples opinion.
Cheers
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Hello,
Thanks for sharing this mind overwhelming discussion.
Obviously, there is no way to solve natural problems except by simplifying them. But if we want to have the closest answer to reality, we should not simplify the matter too much. In my opinion, when we discuss a trait in a single form, we have simplified the issue too much. Because traits are dependent tightly through the different sources, including the genetic correlation, the physical correlation of genes, the LD between several genes based on assortative mattings, and so on. In order that a complex network of the biological pathways will emerge. So just as we are recently dividing the genes that affect a trait into core genes and peripherals, accordingly we can consider pathways to more highlighted and weaker biological pathways, not just ignore them.
Presumably, any simplification and any isolation in biology lead us far from the reality of nature and we know we can not refuse it in our studies.
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The period of fertility in birds is correlated with the population of sperm-filled SSTs in the UVJ.
To achieve fertilization, the resident sperm must be released from the SSTs in order to migrate to the site of fertilization, which is infundibulum part of the oviduct.
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Thanks for your reply. But each of these sources points to several mechanisms. But none of them have identified the most important mechanism. In other words, it is still unclear what is the main mechanism of sperm retention for 14-7 days in the bird's oviduct.
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A lot of factors can be related to litter quality such as, intestinal health, pododermatitis and growth performances. The question is how and if there was more factors which can be related to characteristics of litter.
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Moisture content , texture and pH of litter material are quality indices that will determine the suitability of such materials for chicken litter. Diseases such as coccidiosis, foot pad dermatitis, E.coli can be severe with chicks housed on poor litter materials.
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Would be helpful for writing my dissertation
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Yes, pigs have been used to clear bracken in England.
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I'm conducting an experiment on a flock of 600 chicks. Conditions of environment are controlled, feed and water are given ad libitum. Starting from the second day we noticed that there is a high rate of mortality. After we did some autopsies, we noticed that most of chicks have their vitelline sacs (that was the common abnormality).
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I agree with yahaya
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I am currently in my final year of BSc in Zoo biology and as a part of my course I am hoping to interview people or organisations about the route they took to get to their current career. I am interested in animal conservation, research and laboratory-based fields however, I am open to learn about other fields of animal science as well. If you are interested or know someone who would be, please do not hesitate to contact me here. I will then be sending a short questionnaire (11 questions) to be filled and returned.
I would really appreciate your response and would be grateful for your help!
Bonnie Ikonen
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Hello all,
I have now finished gathering interviews for my final year project, thank you to all who participated in this. This has been a great help for me and I appreciate the time and effort people made!
Kind regards,
Bonnie.
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Animal Science Journals
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Scopes database will be of help
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Call for papers: Aceh Journal of Animal Sciences (free of charges): www.jurnal.unsyiah.ac.id/ajas
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Thank you so much Zainal A. Muchlisin for this valuable information
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I know that some nutritional deficiency, entric diseases or viral infections could result in pica in chickens, but what is the most probable reason if it was not related to nutrition? and what we can do for these chickens?
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Birds start eating materials which are not fit for consumption, such as feathers, litter material, threads, etc. It is less commonly found in modern poultry farm. Phosphorus deficiency, parasitic infestation, new litter material etc. may predispose the birds to pica. Good manage- mental care and balanced diet are recommended for the prevention of pica .
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Science and technology are complementary to each other. The ancient humans developed technologies to survive in earth through different raw materials and tools. They didn't understand the science behind those things. No research was conducted. Everything was generated by trial and error method. Even the animals also go on the same path. I am seeking a constructive discussion regarding arrival of science and technology in human civilization. Which came first?
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Science exists since the day on which universe was created. We may have found the answers to many questions related to science later. But it does not mean that it was not there before. In 1687, Newton presented his theory of universal gravitation and three laws of motion. Does it mean that law of gravity didn't exist before it was demonstrated by Newton?
Science is investigated but technology is created. In the words of Albert Einstein, “Scientists investigate that which already is; engineers create that which has never been.”
Everything can be explained by science. We have not created science, it was there before us and before everything. As per Collins English dictionary, "Technology refers to methods, systems, and devices which are the result of scientific knowledge being used for practical purposes".
So, application of science to develop new devices, systems or methods is called technology.
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Studies of several species reveal a wide spectrum of alterations in mitochondria, mainly during the aging. My interest is in investigating the changes of these alterations in plasma.
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For activity, you may want to look at O2 consumption as mentioned by another comment. For quantifying functional mitochondria, I would recommend using TMRE and mitotracker/JC-1 dyes. TMRE is a dye that is bright under high mitochondiral membrane potential (read: healthy mitochondria) but dim under low mitochondrial membrane potential (read: unhealthy/dying mitochondria). Performing a ratio of TMRE to mitotracker/JC-1 (either will work) will allow you to quantify mitochondrial membrane potential for the total mitochondrial mass.
The linked paper provides a really good summary of different assays to asses mitochondria function and health.
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For my research I need to analyze phytohormome from manure. I am stuck with my procedure. Can anyone suggest best/standard method for quantitative determination of phtyohormone from manure?
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for GA en easy spectrophotmetric method using ammonium amolybdate is easily available on the internet
tell me if you can't find it
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Dear all
I am looking for a reputable journal with a fast feedback from the Editors in Animal Science to publish a Review and my research project paper. I will be very grateful if you could provide me with a list.
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You can try American Journal of Animal and veterinary Science. Fast and reliable in terms of time and credibility
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Please, I want free journals without processing charges in toxicology, poultry science and animal sciences. Thanks.
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Iranian Journal of Applied Animal Science; Genetic Engineering and Biotechnology; Saudi Journal of agricultural sciences
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Hi!
If one wants to use a continuous sampling method for animal behavioural observations and the animal feeds for let's say 15 seconds in a row, then stops feeding for 3-5 seconds because it has been distracted, but then returns to its feeding activity, would one stop coding at 15s or use those 3-5s interruption as a 'tolerance', continue coding and include the 3-5s into the feeding activity?
I can not really find a reference to this and would appreciate if someone could help me on this.
Thanks a lot!
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Following
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AWAY ! Unfortunately.
Nowadays, proximate explanations are, at least almost always, in terms that are neurobiological, endocrinological, or molecular-genetic . There usually appears to be absolutely no concept of a behavioral pattern or change in a behavioral pattern (either, of course, in response to aspects of the current environment) AS themselves a proximate cause of a new behavior pattern [change] -- I.E. a true observable behavior pattern phenomenon proceeding, and needed for, the key subsequent behavior pattern change. I believe there is a BIAS there , due to our philosophical cultural traditional-beliefs.
And, this is a problem.
THIS PROBLEM HAS NOT ALWAYS BEEN THE CASE, and certainly has not always been the case in ethology. The ethology Tinbergen and Lorenz were given a Nobel prize for often did have one behavior pattern as a proximate cause for certain behavior pattern(s) that followed. This is what needs to be re-learned and abided by or real ethology may be lost. Such a relationship between behavior patterns was a hallmark of classical ethology.
Modern ethologists failed to have the "backbone" to maintain that which was most distinctive and best about ETHOLOGY. They basically "caved in" to how others characterized them. (Now, the field is indistinguishable from comparative psychology and/or evolutionary psychology.)
Listen up, International Society for Human Ethology !
Real science, real biological science, the real biology of behavior DEPENDS on behavioral pattern(s), themselves, being seen as a major proximate cause of new behavior patterning [and of behavior pattern change]. Ethology must return to what it uniquely was OR THERE IS NO CHANCE OF BEHAVIORAL SCIENCE. I am sure, if I were a analytic philosopher, I could argue this. It really is logically and scientifically irrefutable. Behavioral sciences, of all "stripes", have been becoming more and more stupid -- there is no better word (since they defy biology and defy science). (Simply look for the lack of the words "behavior pattern" and you are on the way to seeing the whole problem.)
P.S. Consider this a big "kiss ...." to our philosophical cultural heritage; certainly the stupidity is a "love letter" to those arm-chair thinkers.
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I agree with Mr.MacGregor
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Dear all,
Are you aware of data related to the seasonal variation (i.e. weekly or monthly length) of goat hair growth rate. I found several references on cashmere but I'm interested on the normal main hair.
Many thanks in advance !
Rémi
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thank you all for your answers. I found two references mentioning that in both hemisphere guard hair growth more during the warmest months (spring-early autumn) than in the coldest ones (especially winter). However there is no quantitative data on the growth rate difference between the two seasons
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I'm starting a discipline on Ethnozootechnics for graduate students. I am looking for resources in this area. Let me give you some examples:
- McCORKLE, M. An Introduction to Ethnoveterinarian research and development. J. Ethnobiol., v.6, n.1, p.129-149, 1986.
- BOZZOLO, G. Appellations d’origine contrôlée et productions animales : références et guide pratique sur les A.O.C. Paris: Tech. et Doc. Lavoisier, 184p.
- DIGARD, J-P. L'homme et les animaux domestiques. Anthropologie d'une passion. Paris: Fayard, 2009.
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Our journal Ethnoentomology specialises on ethnoentomology and cultural entomology. www.ethnoentomology.cz
The Group of Eastern-European Ethnobiologists is also very interesting. They organise Eastern European Ethnobiology Workshops every couple of years.
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Dear colleagues,
I am searching an appropriate primer for diagnosis of vaginal cancers in female dromedary camels. I encountered some cases with adenocarcinoma, squamous cell carcinoma and some other types of vaginal and cervical cancers. From literature (About half of vaginal cancers in humans is associated with human papillomavirus (HPV) detection, in contrast to cervix cancer which is essentially all associated with HPV). Papillomaviruses have been detected in camels. I tried to test two samples by Prof Robert D Burk (Albert Einstein College of Medicine - NY-USA), but I had a problem in sample transportation. Now, I would like to test the other cases in home, but I do not know what is the appropriate primer that should be used.
Thank you
Ali
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Try FAP59/64 consensus primers or CP4/5 primers.  they are often used to amplify also unknown PVs. I would recommend to perform rolling Circle amplification before subjecting DNa to PCr analysis to enrich circular DNa in the Background of liniear host DNA.
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Is there any available artificial diet for rearing Riptortus pedestris? If yes, could anyone give me a feed formulation and the procedure details for rearing this bug?
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Does anyone know if there is an effect of castration on obstructive urolithiasis development in young lambs/kids?
Or it is mainly related to feed and low calcium to phosphorus ratios?
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Dear Amanda
Please, read on page nine in the following link:
I hope will be useful for you
Good Luck
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Planning to monitor fluctuations in serum kisspeptin levels in periparturient cows and buffaloes. However, I do not know the best method to do this in each species and the most suitable price. Any help?
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Dear Sir, with my respect, the answer is not related to the question.
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Hello, researchers. I'm studying feed hygiene. I am critically looking in the role of the toxic plant  and how do I control for toxic plant  in horse nutrition when a horse is using feed pasture.
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Hello, 
Please see the following article in title Common Toxins in
EQUINE FEEDSTUFFS
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Professor, Associate Professor, Assistant Professor, Lecturer, Assistant Lecturer, Researcher.......
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Thank you very much Dr. Monyer Abd Al-Fatlawi for your help. It's really very useful paper. I appreciate a lot. I worked on "Large-Scale Glycomics of Livestock: Discovery of Highly Sensitive Serum Biomarkers Indicating an Environmental Stress Affecting Immune Responses and Productivity of Holstein Dairy Cows", and would like to continue within the same scope - Thank you! 
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I want to measure in vivo enteric methane emission, but I dont have money for direct tecnique of measure (sf6, infrared laser, etc). I can measure indirectly through equations models? (indirectly from milk production, dry matter intake, body weight... than this information was measure in the experiment). If I can do I it, I have compare diferent models, how select the best model?. I apreciate so much the help.
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Hello Wilson,
There are relatively large number of publications on this topic and few recently published papers are listed below.
As it is clear from these papers, you could use some of the common variables generally recorded in feeding experiments involving cattle (e.g. feed dry matter intake, energy corrected milk yield, body weight, and some key parameters related to feed nutrient composition such as NDF, ADF, EE) to predict enteric CH4 production, provided that original data used for the development of these equations closely represent the management conditions, feeding practices, and productivity of the animals in your experiment. Still, there may always be some errors associated with these predictions (usually, errors in predictions could be up to + or – 30%).
The paper by Escobar-Bahamondes et al (2017) explain well, the methodology you could potentially follow in evaluating an appropriate equation for your condition.
I selected this list, assuming that they might represent your production system.
Although there are large number of equations in the literature, as they have been developed using data from specific regions, you need to be cautious in selecting a suitable equation. Hope this would be helpful for your work. If you can not access any of the papers below, I could send them to you directly. Good luck with your research.
Moraes et al. 2014 Prediction of enteric methane emissions from cattle. http://onlinelibrary.wiley.com/doi/10.1111/gcb.12471/abstract
Patra (2017) Prediction of enteric methane emission from cattle using linear and non-linear statistical models in tropical production systems https://link.springer.com/article/10.1007/s11027-015-9691-7
Escobar-Bahamondes et al. (2017) An evaluation of the accuracy and precision of methane prediction equations for beef cattle fed high-forage and high-grain diet DOI: https://doi.org/10.1017/S175173111600121X
Stergiadis et al (2016) Equations to predict methane emissions from cows fed at maintenance energy level in pasture-based systems http://www.sciencedirect.com/science/article/pii/S0167880915301973
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I would like to measure veterinary antibiotic residues in chicken and swine manure.
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Antibiotic residues in edible animal products are of great concern to regulatory agencies and consumers, so reliable screening methods for rapid, selective and sensitive detection of these residues are necessary to ensure food safety. 
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I've been trying to adapt the widely published MPO-DNA ELISA used to detect human NETs for dog plasma for quite a while without much success and was wondering if anyone had any advice?
I initially used the Roche cell death ELISA plates with the anti-histone antibody from that kit, but with an anti-MPO or anti-elastase as the detector. I got a lot of background, plus marked interference from hemolysis or icterus (which is a problem because I need to able to use this on plasma from sick patients).
I switched to using the same anti-histone clone but without the biotin conjugation, coated overnight onto NUNC ELISA plates. This works ok with the anti-DNA antibody from the Roche Cell death kit, so I can detect nucleosomes and the interference problem is greatly reduced. However, using anti-MPO, or an anti-elastase (sold as cross-reactive with dog, works on immunofluorescence) detected with an appropriate HRP-conjugated secondary and TMB I get a low signal for plasma (about 0.2AU) and even lower for positive controls from canine neutrophils stimulated with PMA (about 0.1AU). I have tried titrating antibodies over a wide range of concentrations and varying incubation times and wash steps. All the buffers are commercial and pH is correct.
Does anyone with experience of running this ELISA in other species have any advice? Thank you
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ttps://www.researchgate.net/.../Tips_for_producing_an_ELISA_for_...
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I need to detect two plasma seminal proteins attached on sperm membrane with inmunofluorescence indirect technique. But doesn't exits any protocol with used of electro-static slides. In the Protocolos used poli-L-lisine slides. 
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Thanks for you help Drs. Mohamed Mahdy, Esraa Tahir  and Massar Saieb. Thanks to you, I was able to make IFI.
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Hi, I am asking about the maximum period of time which the blood sample still fresh and can be used for the RNA extraction, from the collection to the analysis procedure?
Thank you
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mr hussain & Al-sa'aidi, thanks for your comments
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When a young ruminant is born, its rumen is considered a sterile environment that contains no bacteria or other microbial life. The young ruminant is naturally exposed to different microbes through the dam’s birth canal and vagina, saliva, skin and feces. now  we asked about  how diet content affect on rumen development.
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Dear Dr. Hamada
Ruminant livestock are important sources of human food and global greenhouse gas emissions. Feed degradation and methane formation by ruminants rely on metabolic interactions between rumen microbes and affect ruminant productivity. Rumen and camelid foregut microbial community composition was determined in 742 samples from 32 animal species and 35 countries, to estimate if this was influenced by diet, host species, or geography. Similar bacteria and archaea dominated in nearly all samples, while protozoal communities were more variable. The dominant bacteria are poorly characterised, but the methanogenic archaea are better known and highly conserved across the world. This universality and limited diversity could make it possible to mitigate methane emissions by developing strategies that target the few dominant methanogens. Differences in microbial community compositions were predominantly attributable to diet, with the host being less influential. There were few strong co-occurrence patterns between microbes, suggesting that major metabolic interactions are non-selective rather than specific.
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I am studying the vascular adaptive  responses to stroke
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may be ,but in humans more complex , but in the animals specially rodent its an visible.( I  think)  
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What is the best way to measure fecal ETEC shedding in pigs after ETEC challenge from time-effective and result accurate standpoint?
How do I collect and store samples, eg, can I collect fresh fecals on the floor or has to be directly from rectum? Can I store the fecals at -20? 
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from rectum.
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I want to know the best age of insemination of heifer buffaloes?
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Hi
In my opinion, reaching optimal physical condition is more important than age. I agree with Mr. Munir. You should consider all items
Good luck
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buffalo production.
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Hi, I think the same machine  that are used in cows can you use in buffaloes.
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Are they concentrated in specific areas or widespread ? What is their main use? Bactrian camels are a livestock species uniquely able to provide milk and fiber under exceptionally harsh environmental conditions.
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yes Dr Kshash you are right. In early 2014 Dr Bellezza told me that there are rock arts of wild bactrian camels and of domestic bactrian camels in Tibet. Domestic Bactrians camels seems to have been present up to the early historic period 650–1000 AD. 
My interest is on the utilisation of Bactrian camels in today time. Apparently this is not happening......yet !  
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I have already looked at the following publications but the values are expressed differently and difficult to compare:
10.1046/j.1365-2095.1998.00095.x
10.1006/jfbi.1994.1076
10.1023/a:1007757321695
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Am  currently initiating a project in rabbit husbandry and processing of the harvested meat. Am therefore gathering as enough information as possible.
 Thank you!
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Yes it is halal food
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Dr. Britt, 
I ask this question because as you are likely well aware that holsteins are beginning to decline in pedigree depth, health, productive life, and other factors. In your project do you see the holstein breed losing the top number of dairy cows to another breed such as jerseys or even better, brown swiss? I feel between (at least in the U.S.) losing small family farms and more large dairies of 1,000+ cows traits such as productive life, longevity, and etc are becoming the factor that could cause this change. 
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We have many factors affecting the  consistency of milk yield in holstein freisan, Pure breed of holstein freisan will still maintain its productivity provided it is in its natural habitat, but researches exist in order to get better breed, nothing is constant in research, change and discoveries are made daily. 
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I want to get information on a few topics;
firstly; How many million cattle in the world? dairy and beef cattle?
secondly: What is the number of cows applied artificial insemination in the cattle? dairy and beef catlle? therefore, What is artificial insemination success?
thirdly: Do you have experience in embryo transfer in cattle?
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  Dear Dr. Qassim Kshash,
thank you for your link.  I will look at the link (FAO web site)
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I inserted the gene into pGEX-4t-1 and used BL-21 cells for expression after inducing at 37 degree c with 0.1-1M IPTG conc. or 25 degree with 0.5M IPTG. interestingly, my control group can expressed GST tag normally, but there is nothing for my GST-fusion proteins. I sequenced my recombinant plasmid, the whole ORF (GST-thrombin-my ORF) was 100% correct. In consideration of the rare coden and protein toxicity, i  also choosed Rosetta and pLsS cell, but the recombinant protein still didn't express.What can I do to solve this problem?
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group can expressed GST tag normally, but there is nothing for my GST-fusion proteins. I sequenced my recombinant plasmid, the whole ORF (GST-thrombin-my ORF) was 100% correct. In consideration of the rare coden and protein toxicity, i also choosed Rosetta and pLsS cell, but the recombinant protein still didn't express.What can I do to solve this problem
Why don't my zebrafish proteins express when transforming my cDNA-pGEX vector in BL21 cells? - ResearchGate. Available from: https://www.researchgate.net/post/Why_dont_my_zebrafish_proteins_express_when_transforming_my_cDNA-pGEX_vector_in_BL21_cells [accessed Mar 21, 2017].
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There are 4 views that should be taken of an equine thorax: 1) craniodorsal, 2) caudodorsal, 3) cranioventral, and 4) caudoventral.
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There are 4 views that should be taken of an equine thorax: 1) craniodorsal, 2) caudodorsal, 3) cranioventral, and 4) caudoventral.
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animal science
animal nutrition
biochemist
chemical engineer
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Assuming that methylesters wil be split to liberate the fatty acids, which seems a reasonable assumption as esters can be split rather easily, there is no reason to assume that the fate of the fatty acids in the intestine (and the body) will be any different than when feeding free fatty acids.
Young animals will be more susceptible to high fat levels in the diet, so that might be an important limitation. The attached file gives some (older) information on e.g. feeding values of fats in poultry
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in commercial markets there is product contain kanamycine and colestin suitable for ruminant , can I give it to horse as label off item?
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it  nephorotoxic 
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None
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Thanks Dr Ashraf
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Looking for feedback, positive or negative, from anyone who has used the LSBio.com bovine prolactin ELISA kit.
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We opted to try a kit from Aviva Systems.  Standards seem to be working well for us but we are having some issues with the sample matrix.  We're now in the process of working out some hook effect issues that we've been having.   Once we hit the correct dilution we should be good to go. I appreciate all the info and assistance.
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Hi all. Is anybody of you experienced with measuring folate levels in mouse plasma? If yes, which method do you usually use and what levels do you expect in a healthy adult mouse? Thanks lots for your help!
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Dear Dr. Ulrike Weber ,, this link can help you
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Growsafe equipment is used for feedlot animals to measure individual animal's feed intake....I am wondering there is such a probable system for grazing animals
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Dear Robert,
This is not an easy task. The sward methods are often not accurate, mainly with multispecies prairies. Please have a look at publications on the use of n-alkanes as internal markers to estimate diet composition, intake and digestibility. The technique has its odds, but could be used under well defined conditions.
Best regards
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Hi
I analzysed 15 populations and 10 microsat loci with Arlequin ver 3. I have already got observed and expected heterozygosity and corresponding P-value.
How can I perform Bonferroni correction for those data? However, there was no option to fix P-value in Arlequin soft. for like 0.05/0.1 before analyzing data. So, which P-value i need to select for Bonferroni correction?
Another question is that
in some cases, the value for observed heterozygosity was greater than expected heterozygosity in some loci. Is that affect my result and how?
Please, give some explanation.
I have attached the HWE file here. Please explain the bonferroni correction method with my example data.
Thanks
Rakeb
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Hi Daniel
Thank you very much for your help. From my data I got the result as follows after running both.
p.adjust(c(0.14057,0.04561,0.0309,0.02863,0.07182,0.00066,0.21414,0.02691,0.00006,0.06448,0.0002),method="bonferroni")
[1] 1.00000 0.50171 0.33990 0.31493 0.79002 0.00726 1.00000
[8] 0.29601 0.00066 0.70928 0.00220
> p.adjust(c(0.14057,0.04561,0.0309,0.02863,0.07182,0.00066,0.21414,0.02691,0.00006,0.06448,0.0002),method="holm")
[1] 0.28114 0.22805 0.21528 0.21528 0.25792 0.00594 0.28114
[8] 0.21528 0.00066 0.25792 0.00200
However, my P-value for this data was 0.05. So, How do I know that which is significant? Is that the value which is less than 0.005?
Please let me know
Thanks
Rakeb
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Hi everybody,
Is anyone aware of harmful effects of colostrum feeding to newborn lambs? Some of our clients are claiming that if they properly feed their pregnant ewes during the last month of gestation, most of those lambs would face with undigested and coagulated colostrum in abomasum, resulting in bloat, feebleness, and finally death in 2-3 days. Do you have any similar experience? Is it because of high fat or energy colostrum? Any comments?
Ehsan 
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Dear Ehsan
Long time ago, I saw calves dying because of their mothers eating ammonia-treated grass, which as a result produced 4-metylimidazole in the milk... as far as I remember. Maybe the same issue here?
Kind regards Lene
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EHV: Equine Herpes Virus
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Wikipedia: EHV may stand for
Education on Human Values, Education on Human Values a methodology for inculcating Values in Human .
Extra high voltage, a type of power supply
Equine herpesvirus, a group of viruses that affect horses
and much more
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I need some tlr mutant strains of mice. Can anyone help me find these mice in India?
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TLR 2 ko mice are present in ICGEB, you can contact them.
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I want to use Cryotop technique for embryo vitrification. Did you have any experience about this issue? I know that Kitazato has commercial kits for this aim. Have you used this product? Or can we prepare vitrification solutions or straws for this aim?
Thank you for your answers... 
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Hello
We used Cryotop of open-type. we prepared solutions ourselves but used Kitazato Cryotop. you can vitrified 5 embryos for each straw  for animal embryos. your skill is very important and affected on viability and developmental rate of embryos.
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Dear researchers,
Does anyone have any reference or an article (review) about the types of intestinal amino acid transporter and its actions in poultry other than article entitled absorption of amino acids and peptides by C. R. Krehbiel and J. C. Matthews).
Regards
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Dear Khalid Hassan,
Thank you for your suggestions.
But as I say I need review about amino acid transporters.
Best regards
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Excuse me,
I have some cases of abortion in late pregnant ewes and others are still birth, some of them suffer from arthritis, I am thinking about chlamydial abortion could you tell me what are the most characteristic gross and post-mortem lesions in the dead foetuses caused by Chlamydia?
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To add to the comments above, 1.It is fairly common practice to inject all the pregnant ewes that are left with long acting tetracycline immediately. This "in the face of an outbreak" treatment  is surprisingly effective. The ewes must be handled with care , use sterile needles new antibiotic etc 2. It is a zoonosis that is quite efficient at spreading . Thought must be given if any women of child bearing age are involved in any way. https://www.gov.uk/guidance/chlamydophila-abortus
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Does anyone know the most appropriate and affordable method of studying the motion of an entire or part of the body of an animal?
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Dear Miriam,
Good day. I have contacted the company you referred me to. I saw their product called equimetrix. However, it is very expensive (12, 500 euros). Please, can you suggest any other alternative which may be cheaper and affordable? 
Kind regards,
Shade.
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We are seeing gizzard erosions in very young broilers. They are less than 7 days old. What could be the cause of it? On the Elanco scoring system it will be mostly 1 and 2 scores.
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Dear Joline,
On corn-soybean meal the most frequent cause under my experience is soybean meal quality: mainly underheated soybean meal. However, overheated soybean meal has also shown to induce gizzard erosion.
Regards 
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It is not fresh pit, Re-inoculation is done
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It could be because of function of Azolla eubacteria and some chemicals (especially KOH) in the water. To get rid of that maybe use of DD water could be helpful.
For better understanding take a look @ attached file.
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I wanna ask if Metformin oral solutions such as glucophage sachet or riomet solution can be used for intraperitoneal injection in my In-vivo trial of hamsters infected with prion? and what if i crush the tablet and make solution in 0.9% saline? which one is better? 
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well thanks alot morton
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I'm looking to administer estriol over a long period of time (like more than a year). Multiple pellet implantations is not really feasible because it would be really bad for the mice. Also IP injections daily for more than a year would be tiring for me and stressful for the mice. So basically I'm curious if there is a way to continuously give the mice estriol through food or water. 
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Another feature is oral bio-availability vs implant bio-availability of hormones.
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Can anyone help me to found any article related with the Dairy Sheep Omega-3 Feeding and Body Conformation and body coundation score?
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Article: AENSI Journals Advances in Environmental Biology Corresponding Author: Growth Performance and Conjugated Linoleic acid (CLA) Content on Meat of Growing Lambs Fed Diets Containing Vegetable Oils
Eman H El-Sabaawy · Sawsan M Gad · T M El-Bedawy · [...] · A M Abd El-Gawad
RESEARCHGATE : Taha Elbedawy
Full-text Article · Aug 2015 · Advances in Environmental Biology
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How to estimate the age of rodents and carnivores?
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There are several methods that can be applied to dead individuals or museum specimens (skeletons). In the latter case, the analyses of tooth wear or baculum (os penis) in carnivores, for instance. The analysis of the dry weight of the eye lens or the analysis of the growth of the long bones are others.
This review is old, but recent literature on this topic, as to my knowledge, is scanty: 
Morris, P. (1972). A review of mammalian age determination methods. Mammal review, 2(3), 69-104. 
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Theoretically yes, but it is very risky to depend only on lab determination. like if your materiel has high value of phenolic compounds,high EE% even high NDF, all of them get high value of gross energy and did not have positive correlation with ME. in my work i determined gross energy in rice straw  and barely grain  as 3.10 and 3.44 Mcal/Kg respectively. So at least you can using in vitro test (using enzymes). good luck    
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I am currently reviewing the diets of some primate species (starting with the callitrichids) in a zoological collection. I am curious to see how it compares to other diets (natural and in a captive situation).
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Nutrition chapter, in Non humans primates in biomedical research will give you a nutrition review in pirmates and multiple references to follow
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Food safety is a concern for many consumers and adulteration of food of animal origin becomes a widespread practice in countries lacking food control authorities.
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I apologize for my insistence, but in such circumstances the techniques based on polymerase chain reaction (PCR) is effective...
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