Questions related to Animal Science
I would like to know how much long can I conserve serum samples in - 20°C for biochemical analysis : lipid profile (cholesterol, triglyceride...), protein profile (albumin, total protein...), enzymatic (ASAT, ALAT...), glucose .
Thank you in advance
I used the Latex template of JASB (Journal of Animal Science and Biotechnology) to submit a manuscript. The produce PDF using overleaf looks pretty well and the initial submission went well. For the revised version, the journal asked to upload the PDF and the latex files (e.g. .tex, .bib, .cst).
I realised that there were some discrepancies with the references and it took me a while to made some minor changes to the template but I finally succeeded to get it right (i.e., in some cases I needed to change the information of the .bib file, I needed to point to the vancouver.cst file in the \bibliographystyle command, and I edited the bmcart.csl file in order to use comas instead of semicolon between keywords of the abstract).
I compiled latex in overleaf and do not have any 'red' warnings and the PDF came out perfectly. But, I do get overfull and underfull warnings for hbox and vbox, for websites references says author is missing.
When I upload the files into the editorial system of the journal, the compilation does not work well and the built document does not really show my manuscript but errors.
Does anyone have recommendations/tips to get this submission using Latex right? Otherwise, it seems that I will need to make a word document, which would be a large waste of time.
Thank you in advance!
I need a list or site where I can easily find journals with no APC. The journals' scope should be Animal sciences, Biotechnology, Genetics and Molecular Biology.
This questionnaire is for dog owners. This is part of my disseration for animal science. I am looking to see if canines with anxiety are more at risk of becoming ill. The data will only be used for the purpose of this disseration. even if your dog does not have any anxiety please fill in as it may just prove anxiety has no effect on a dogs health.
This is a common practice to prefix Dr. After completing PhD in any discipline. May be science arts or commerce. This really creates confusion many times.
Peers plz suggest and if possible may something be done to eliminate this confusion . this is a personal query. nothing personal to anybody. Just enlight what you feel.
An extensive systematic search resulted in an overview of all the animal victims from covid litter found worldwide, including entanglements, ingestions, and entrapments. Thanks to nature photographers, animal rescue centers, birdwatchers and litter pickers, we learned a great deal about just how harmful PPE products can be. Monkeys, fishes, hedgehogs, penguins, crabs, and many more animals are affected, even pets like cats and dogs.
Share your observations!
We started a database where anyone can share further observations of interactions between animals and PPE litter. As we only searched for articles in English and Dutch and found mainly cases from the UK, USA, and The Netherlands, we expect that there must be many more examples! Feel free to add your own observations or add new examples - from your local or national news, or social media - that are currently missing in the global overview. Let us know!
Find the overview at: www.covidlitter.com
I am doing an allantoin assay in urine and all the protocols seem pretty vague. I am currently using this website which seems to be the most helpful:
It mentions that I use 15 mL tubes, but since I am boiling the samples should I use glass tubes with a cap to prevent escape? Or can I just use plastic tubes like the ones here:
I’m new in the field of measuring heart rate and heart rate variability. I’m particularly interested in measuring heart rate in dogs and sheep in connection to stress. I would be very grateful for some advice regarding the equipment for monitoring the heart rate-what monitors do you use, what do I have to pay attention to (there are so many heart beat monitors on the market-which one is good and why…)?
During publishing an article what is the permissible range of plagiarism.?.
Many times we publish more than one articles from a sinhle study where the study and materials and methods are almost same. In biological science clubbing more parameters creates confussion amongst the readers and also to draw the conclusion of the finding. And in most of the journals there is a set word limit. So mostly we cant place the whole study in one article. Thus we split into many depending the extent of study. But there cones the plagiarism while publishing. As most of the methods like feed preparation feeding procedures etc so automatically it seems to be high familiarity index but originally the study is novel. Hence please guide the researchers in this regards how this can be eliminated. .. and a request to editors can similarity index be considered for intro results and discussion only. Even references may give rise to higher similarity index.
Please guide us.
I've measured stable C and N isotopes in sequential segments of dingo vibrissae, and hope to determine whether there are any seasonal patterns in their diet. To really do this analysis justice I need to know the growth rates of vibrissae (preferably mystacial). Data from a Canis sp. would be best but another genus will do if they're not available. Thanks for your time.
I want to install GPS trackers on hare (Lepus timidus).
What I need is probably an GPS/GSM logger, weight approx. <50 g
· Is there someone here, having experience in this kinds of work?
· How works the GPS-collar – What is the price - How reliable are they?
· Is it some company/web page you can recommend?
Sometimes interpreting biological terms/pathways related to a particular phenotype might be very difficult and confusing. I have read many papers which only discussed a few selections of pathways related to their objectives of study and simply ignored the rest of pathways and terms (buried in supplementary materials!). This is an issue which needs an urgent solution. In Animal Science, we work with traits e.g. milk production or birth weight which are complex traits and multiple genes involved in the whole process. Although through GWAS studies we know most of genes involved in a complex trait. To my knowledge the "gap" is: there is no direct biological pathway (for example in Cytoscape or David) related to milk production.
I know "mammary gland development" or "cell-cell junctions" or other known terms, together are related to milk production but they need to be altogether in one term. This was only one example among many other important traits.
I would like to hear other peoples opinion.
The period of fertility in birds is correlated with the population of sperm-filled SSTs in the UVJ.
To achieve fertilization, the resident sperm must be released from the SSTs in order to migrate to the site of fertilization, which is infundibulum part of the oviduct.
A lot of factors can be related to litter quality such as, intestinal health, pododermatitis and growth performances. The question is how and if there was more factors which can be related to characteristics of litter.
I'm conducting an experiment on a flock of 600 chicks. Conditions of environment are controlled, feed and water are given ad libitum. Starting from the second day we noticed that there is a high rate of mortality. After we did some autopsies, we noticed that most of chicks have their vitelline sacs (that was the common abnormality).
I am currently in my final year of BSc in Zoo biology and as a part of my course I am hoping to interview people or organisations about the route they took to get to their current career. I am interested in animal conservation, research and laboratory-based fields however, I am open to learn about other fields of animal science as well. If you are interested or know someone who would be, please do not hesitate to contact me here. I will then be sending a short questionnaire (11 questions) to be filled and returned.
I would really appreciate your response and would be grateful for your help!
I know that some nutritional deficiency, entric diseases or viral infections could result in pica in chickens, but what is the most probable reason if it was not related to nutrition? and what we can do for these chickens?
Science and technology are complementary to each other. The ancient humans developed technologies to survive in earth through different raw materials and tools. They didn't understand the science behind those things. No research was conducted. Everything was generated by trial and error method. Even the animals also go on the same path. I am seeking a constructive discussion regarding arrival of science and technology in human civilization. Which came first?
Studies of several species reveal a wide spectrum of alterations in mitochondria, mainly during the aging. My interest is in investigating the changes of these alterations in plasma.
For my research I need to analyze phytohormome from manure. I am stuck with my procedure. Can anyone suggest best/standard method for quantitative determination of phtyohormone from manure?
I am looking for a reputable journal with a fast feedback from the Editors in Animal Science to publish a Review and my research project paper. I will be very grateful if you could provide me with a list.
Please, I want free journals without processing charges in toxicology, poultry science and animal sciences. Thanks.
If one wants to use a continuous sampling method for animal behavioural observations and the animal feeds for let's say 15 seconds in a row, then stops feeding for 3-5 seconds because it has been distracted, but then returns to its feeding activity, would one stop coding at 15s or use those 3-5s interruption as a 'tolerance', continue coding and include the 3-5s into the feeding activity?
I can not really find a reference to this and would appreciate if someone could help me on this.
Thanks a lot!
AWAY ! Unfortunately.
Nowadays, proximate explanations are, at least almost always, in terms that are neurobiological, endocrinological, or molecular-genetic . There usually appears to be absolutely no concept of a behavioral pattern or change in a behavioral pattern (either, of course, in response to aspects of the current environment) AS themselves a proximate cause of a new behavior pattern [change] -- I.E. a true observable behavior pattern phenomenon proceeding, and needed for, the key subsequent behavior pattern change. I believe there is a BIAS there , due to our philosophical cultural traditional-beliefs.
And, this is a problem.
THIS PROBLEM HAS NOT ALWAYS BEEN THE CASE, and certainly has not always been the case in ethology. The ethology Tinbergen and Lorenz were given a Nobel prize for often did have one behavior pattern as a proximate cause for certain behavior pattern(s) that followed. This is what needs to be re-learned and abided by or real ethology may be lost. Such a relationship between behavior patterns was a hallmark of classical ethology.
Modern ethologists failed to have the "backbone" to maintain that which was most distinctive and best about ETHOLOGY. They basically "caved in" to how others characterized them. (Now, the field is indistinguishable from comparative psychology and/or evolutionary psychology.)
Listen up, International Society for Human Ethology !
Real science, real biological science, the real biology of behavior DEPENDS on behavioral pattern(s), themselves, being seen as a major proximate cause of new behavior patterning [and of behavior pattern change]. Ethology must return to what it uniquely was OR THERE IS NO CHANCE OF BEHAVIORAL SCIENCE. I am sure, if I were a analytic philosopher, I could argue this. It really is logically and scientifically irrefutable. Behavioral sciences, of all "stripes", have been becoming more and more stupid -- there is no better word (since they defy biology and defy science). (Simply look for the lack of the words "behavior pattern" and you are on the way to seeing the whole problem.)
P.S. Consider this a big "kiss ...." to our philosophical cultural heritage; certainly the stupidity is a "love letter" to those arm-chair thinkers.
Are you aware of data related to the seasonal variation (i.e. weekly or monthly length) of goat hair growth rate. I found several references on cashmere but I'm interested on the normal main hair.
Many thanks in advance !
I'm starting a discipline on Ethnozootechnics for graduate students. I am looking for resources in this area. Let me give you some examples:
- Société d'Ethnozootechnie (http://www.ethnozootechnie.org/presentation) with their Newsletter (http://www.ethnozootechnie.org/publications/la-lettre-de-la-sez/), Magazine (http://www.ethnozootechnie.org/publications/revue-ethnozootechnie/) and others documents.
- McCORKLE, M. An Introduction to Ethnoveterinarian research and development. J. Ethnobiol., v.6, n.1, p.129-149, 1986.
- BOZZOLO, G. Appellations d’origine contrôlée et productions animales : références et guide pratique sur les A.O.C. Paris: Tech. et Doc. Lavoisier, 184p.
- DIGARD, J-P. L'homme et les animaux domestiques. Anthropologie d'une passion. Paris: Fayard, 2009.
I am searching an appropriate primer for diagnosis of vaginal cancers in female dromedary camels. I encountered some cases with adenocarcinoma, squamous cell carcinoma and some other types of vaginal and cervical cancers. From literature (About half of vaginal cancers in humans is associated with human papillomavirus (HPV) detection, in contrast to cervix cancer which is essentially all associated with HPV). Papillomaviruses have been detected in camels. I tried to test two samples by Prof Robert D Burk (Albert Einstein College of Medicine - NY-USA), but I had a problem in sample transportation. Now, I would like to test the other cases in home, but I do not know what is the appropriate primer that should be used.
Does anyone know if there is an effect of castration on obstructive urolithiasis development in young lambs/kids?
Or it is mainly related to feed and low calcium to phosphorus ratios?
Planning to monitor fluctuations in serum kisspeptin levels in periparturient cows and buffaloes. However, I do not know the best method to do this in each species and the most suitable price. Any help?
Hello, researchers. I'm studying feed hygiene. I am critically looking in the role of the toxic plant and how do I control for toxic plant in horse nutrition when a horse is using feed pasture.
I want to measure in vivo enteric methane emission, but I dont have money for direct tecnique of measure (sf6, infrared laser, etc). I can measure indirectly through equations models? (indirectly from milk production, dry matter intake, body weight... than this information was measure in the experiment). If I can do I it, I have compare diferent models, how select the best model?. I apreciate so much the help.
I've been trying to adapt the widely published MPO-DNA ELISA used to detect human NETs for dog plasma for quite a while without much success and was wondering if anyone had any advice?
I initially used the Roche cell death ELISA plates with the anti-histone antibody from that kit, but with an anti-MPO or anti-elastase as the detector. I got a lot of background, plus marked interference from hemolysis or icterus (which is a problem because I need to able to use this on plasma from sick patients).
I switched to using the same anti-histone clone but without the biotin conjugation, coated overnight onto NUNC ELISA plates. This works ok with the anti-DNA antibody from the Roche Cell death kit, so I can detect nucleosomes and the interference problem is greatly reduced. However, using anti-MPO, or an anti-elastase (sold as cross-reactive with dog, works on immunofluorescence) detected with an appropriate HRP-conjugated secondary and TMB I get a low signal for plasma (about 0.2AU) and even lower for positive controls from canine neutrophils stimulated with PMA (about 0.1AU). I have tried titrating antibodies over a wide range of concentrations and varying incubation times and wash steps. All the buffers are commercial and pH is correct.
Does anyone with experience of running this ELISA in other species have any advice? Thank you
I need to detect two plasma seminal proteins attached on sperm membrane with inmunofluorescence indirect technique. But doesn't exits any protocol with used of electro-static slides. In the Protocolos used poli-L-lisine slides.
Hi, I am asking about the maximum period of time which the blood sample still fresh and can be used for the RNA extraction, from the collection to the analysis procedure?
When a young ruminant is born, its rumen is considered a sterile environment that contains no bacteria or other microbial life. The young ruminant is naturally exposed to different microbes through the dam’s birth canal and vagina, saliva, skin and feces. now we asked about how diet content affect on rumen development.
What is the best way to measure fecal ETEC shedding in pigs after ETEC challenge from time-effective and result accurate standpoint?
How do I collect and store samples, eg, can I collect fresh fecals on the floor or has to be directly from rectum? Can I store the fecals at -20?
Are they concentrated in specific areas or widespread ? What is their main use? Bactrian camels are a livestock species uniquely able to provide milk and fiber under exceptionally harsh environmental conditions.
I have already looked at the following publications but the values are expressed differently and difficult to compare:
Am currently initiating a project in rabbit husbandry and processing of the harvested meat. Am therefore gathering as enough information as possible.
I ask this question because as you are likely well aware that holsteins are beginning to decline in pedigree depth, health, productive life, and other factors. In your project do you see the holstein breed losing the top number of dairy cows to another breed such as jerseys or even better, brown swiss? I feel between (at least in the U.S.) losing small family farms and more large dairies of 1,000+ cows traits such as productive life, longevity, and etc are becoming the factor that could cause this change.
I want to get information on a few topics;
firstly; How many million cattle in the world? dairy and beef cattle?
secondly: What is the number of cows applied artificial insemination in the cattle? dairy and beef catlle? therefore, What is artificial insemination success?
thirdly: Do you have experience in embryo transfer in cattle?
I inserted the gene into pGEX-4t-1 and used BL-21 cells for expression after inducing at 37 degree c with 0.1-1M IPTG conc. or 25 degree with 0.5M IPTG. interestingly, my control group can expressed GST tag normally, but there is nothing for my GST-fusion proteins. I sequenced my recombinant plasmid, the whole ORF (GST-thrombin-my ORF) was 100% correct. In consideration of the rare coden and protein toxicity, i also choosed Rosetta and pLsS cell, but the recombinant protein still didn't express.What can I do to solve this problem?
Looking for feedback, positive or negative, from anyone who has used the LSBio.com bovine prolactin ELISA kit.
Hi all. Is anybody of you experienced with measuring folate levels in mouse plasma? If yes, which method do you usually use and what levels do you expect in a healthy adult mouse? Thanks lots for your help!
Growsafe equipment is used for feedlot animals to measure individual animal's feed intake....I am wondering there is such a probable system for grazing animals
I analzysed 15 populations and 10 microsat loci with Arlequin ver 3. I have already got observed and expected heterozygosity and corresponding P-value.
How can I perform Bonferroni correction for those data? However, there was no option to fix P-value in Arlequin soft. for like 0.05/0.1 before analyzing data. So, which P-value i need to select for Bonferroni correction?
Another question is that
in some cases, the value for observed heterozygosity was greater than expected heterozygosity in some loci. Is that affect my result and how?
Please, give some explanation.
I have attached the HWE file here. Please explain the bonferroni correction method with my example data.
Is anyone aware of harmful effects of colostrum feeding to newborn lambs? Some of our clients are claiming that if they properly feed their pregnant ewes during the last month of gestation, most of those lambs would face with undigested and coagulated colostrum in abomasum, resulting in bloat, feebleness, and finally death in 2-3 days. Do you have any similar experience? Is it because of high fat or energy colostrum? Any comments?
I want to use Cryotop technique for embryo vitrification. Did you have any experience about this issue? I know that Kitazato has commercial kits for this aim. Have you used this product? Or can we prepare vitrification solutions or straws for this aim?
Thank you for your answers...
I have some cases of abortion in late pregnant ewes and others are still birth, some of them suffer from arthritis, I am thinking about chlamydial abortion could you tell me what are the most characteristic gross and post-mortem lesions in the dead foetuses caused by Chlamydia?
Does anyone know the most appropriate and affordable method of studying the motion of an entire or part of the body of an animal?
We are seeing gizzard erosions in very young broilers. They are less than 7 days old. What could be the cause of it? On the Elanco scoring system it will be mostly 1 and 2 scores.
I wanna ask if Metformin oral solutions such as glucophage sachet or riomet solution can be used for intraperitoneal injection in my In-vivo trial of hamsters infected with prion? and what if i crush the tablet and make solution in 0.9% saline? which one is better?
I'm looking to administer estriol over a long period of time (like more than a year). Multiple pellet implantations is not really feasible because it would be really bad for the mice. Also IP injections daily for more than a year would be tiring for me and stressful for the mice. So basically I'm curious if there is a way to continuously give the mice estriol through food or water.
Can anyone help me to found any article related with the Dairy Sheep Omega-3 Feeding and Body Conformation and body coundation score?
I am currently reviewing the diets of some primate species (starting with the callitrichids) in a zoological collection. I am curious to see how it compares to other diets (natural and in a captive situation).
Food safety is a concern for many consumers and adulteration of food of animal origin becomes a widespread practice in countries lacking food control authorities.