Questions related to Animal Physiology
My SD rats have fasting blood sugar (16-25 mmol/l) after 6 weeks of STZ-induced diabetes (60 mg/kg) . Can anyone give me the range for FBG in STZ-induced rats?
What are the Minimum required samples for RNA-seq Analysis in poultry diseases (for example, ascites)?
Hello everyone, I have encountered some problems in research, I hope someone can help me. In the blood biochemical analysis of my broiler experiment, the AST (aspartate aminotransferase) of the treatment group was significantly higher than that of the positive control group, but there was no significant difference in GGT, LDH and CK. There was no significant difference in growth traits. Therefore, it seems that the treatment group did not cause physiological adverse effects. In the carcass traits, the breasts relative weight of the treatment group was significantly higher than that of the positive control group. Then can I infer whether AST can be used as a positive indicator of animal growth?
I read a few articles related infant & dog's auditory preference. They both mentioned "baby talk" could be more attractive to infant & dog. And my team and I want to find out the reason from evo-psych. We already know one of necessary condition is high pitch, and had some data about high pitch preference for dogs (we took gaze duration to be parameter).
Our project focus on evo-psych puzzle, so we want to exclude the physical or physiological reason to explain why dog prefer high pitch. I tried to do some reviews to know physical trait of high pitch. But I almost found nothing help because I barely know the domain knowledge of acoustics. So if physical trait of high pitch could explain why mammal like human and dog has such preference, then we should reschedule our project.
Thank you for response such fundamental question.
As we know researches are being rapidly evolved in most majors. I would like to know where animal reproduction physiology researches will go to in future. where do animal reproduction physiologist see themselves in 5 to 10 years later?
In my opinion, it is vital for researchers in this field to find out which aspects of their major define future studies and accordingly researchers should empower their knowledge in growing areas.
It is my pleasure to know your idea about the future of animal reproduction physiology researches and related areas.
I would appreciate if any related papers, sites and other stuffs be suggested.
I’m new in the field of measuring heart rate and heart rate variability. I’m particularly interested in measuring heart rate in dogs and sheep in connection to stress. I would be very grateful for some advice regarding the equipment for monitoring the heart rate-what monitors do you use, what do I have to pay attention to (there are so many heart beat monitors on the market-which one is good and why…)?
Hello, I am looking for a publication that would allow me to estimate the metabolic mass of young mice, from their birth to adulthood.
Do you know of any publications?
Please share the materials and views on canine and feline feeding. In recent times owners prefer to consult doctors more frequently regarding dog foods, quantity to be offered ingredients etc. And many myths are also associated like salt feeding sugar feeding even people dont like to offer milk to dogs. Please put some light and share your experiences in pet feeding.
Please suggest a balanced feedung schedule for dogs and cats.?
Thanks and regards
I work with female mice and I have this doubt. If they are all put together on the same cage, are all of them on the same estrous cycle stage?
If so, is there any literature that supports this?
I do not measure and/or calibrate estrous cycle because I do a protocol where I don't want the animals to be stressed by it.
The period of fertility in birds is correlated with the population of sperm-filled SSTs in the UVJ.
To achieve fertilization, the resident sperm must be released from the SSTs in order to migrate to the site of fertilization, which is infundibulum part of the oviduct.
Dear RG Colleagues,
When we start a research project with the purpose of publishing and sharing the results in a scientific journal, we are often asked to give the geographical coordinates of our study site where the species has been observed and sampled. The problem is here, when the study concerns a very rare species (animal or plant). My question is: How can we publish works (including the sampling site) on a protected species by preventing it from being annihilated forever by poachers (collector, destructive study, herbalist ...). Lately, I've started to observe and photograph every plant and animal species I found in my region (Mediterranean basin: aquatic and terrestrial environment). I asked myself this question a long time ago because there are endangered species but they continue to survive in secret where people do not have access to find them. Do you agree with me ?!, to be a SELFISH, to admire these species in secret, to never divulge their existence … and avoid seeing them disappear forever…
Thank you for your attention
I am looking for a book about the concept of transition period in dairy animals and its related metabolic and oxidative challenges.
Any help will be greatly appreciated.
I'm trying to do research into Behavioural Endocrinology, as I am considering it for my final project for my masters. But I can't seem to find information anywhere, regarding equipment etc. Any help would be appreciated!
I have been perfusing isolated hearts with KHB supplemented with 4% BSA. With normal KHB I never encountered problems but with BSA some kind of circulatory blockage seems to set in after a few minutes (coronary flow rates drop at constant pressure; pressure rises at constant flow) and the heart enters asystole within ~20min. I have excluded bubbles and other common problems with these preparations as causes.
The only factor determining failure seems to be BSA. I am using plain molecular biology grade one. Some papers I read use fatty acid free BSA, others not. Is purity or other factors an important consideration?
Thank you for all your help.
I know it is a carbocyanine-based Mitotracker. Unlike other MTKs like MTK Orange, it doesn't become fluorescent upon oxidation or else. Does anybody have any information regarding how this molecule becomes fluorescent?
I'm trying to install it but it seems the installation process cannot be completed. I follow all the instructions in the .txt doc but still, the .exe file does not run. Has anyone succesfully installed and tested it?
I would like to start a new research project in which, according to recent articles, I should administer FA through drinking water. I have read a lot of articles that have administered FA through that protocol, but they did not report details. I know the theory from literature, but practical tips would help a lot definitely.
On a footnote, the drinking water protocol is different from gavage!
I hope you could help me with the methodology.
This is one aspect of reproduction that has no symptoms and is hard to diagnose or detect.
I am going to treat the myocardial infarction rats with spironolactone for 8 weeks/56 days. As you know, there are several ways to administer this drug, such as oral (gavage and mixed in rat chow), intraperitoneal and subcutaneous injection. We currently cannot mix it with rat chow.
Which technique do you think is more prior?
How to make the solution of it? Can we make the solution with physiological serum?
Your advice and suggestions will be much appreciated and welcomed.
It is possible to determine some markers of oxidative stress or generally animal condition for single gammarid? If yes could you suggest some method?
We want to determinate electrolytes (among other things) in rats with diabetes insipidus secondary to the removal of the neurohypophysis but we are having problems with urine Na and Cl electrolytes.
I am not a researcher investigating animal behavior, but I need some data and impression from the field.
From evolutionary perspective, weak individuals, for example these who are injured have less chance to survive.
Is it the case, that in some species (I am not talking about humans now), others take care about injured, weakened individuals thus increasing the chances to survive? Which species exhibit such a behavior?
To simplify, are there examples in animal world that some representatives of e.g primates take care about injured, weakened individuals?
I am going to induce diabetes in C57/BL6 mice using a high dose of (150 mg/Kg) STZ. I should be able to keep them for 16 weeks after induction of diabetes.
May my potential dose have a lot of mortality rate?
Is it better to have anesthetized mice at the time of intraperitoneal injection?
What are the solutions to prevent the death of mice over the project? I know the theory of literature, but practical tips would help a lot definitely.
I would be grateful if you could give me anything about the induction of diabetes by a single dose of STZ.
I am going to induce acute myocardial infarction in rats by LAD ligation. We do not have access to ECHO and ECG to confirm the MI model. Does the observation of damaged area (color changes) after LAD ligation confirm our model?
I am just looking for MI-induced fibrosis.
I would be very grateful if you could give me some advice.
I am looking for a rat somatostatin (SST) 96 well plate ELISA with a required sample (plasma) volume of 10-20 µl, preferably from a US/Canada based supplier. All SST ELISA kits that I have been working with so far require a sample volume of 50µl in duplicates.
I know that in the case of small volume samples, original samples can be diluted to solve the problem, but I'm trying to avoid dilutions as much as possible, so instead I'm hoping to find an alternative ELISA with the minimum undiluted sample volume required.
Thank you in advance,
We are interested to show evolutionary functionality of the circadian system. Specifically we are now interested whether timing of male display (both seasonal and daily timing) might be generate more attraction for females and possibly lead to more offspring with higher survival. We think about systems like morning song in birds (dawn chorus) or roaring behaviour in red deer. There may be many more examples out there, but the literature is not easily disclosed. We would love to obtain insights from specialists in these behaviours.
In the Paxinos & Watson mouse atlas, each page/slice has a coordinate grid and is referenced to some landmark (for coronal, A/P relative to bregma).
The reference mouse atlas from the Allen Institute is available as segmented 2D figures, and also in 3D (Brain Explorer 2).
Is there a way to determine the stereotaxic coordinates of a point in the Allen mouse atlas (either in the 2D slice viewer, or the 3D viewer), referenced to anatomical landmarks (such as bregma)?
Hi all. Is anybody of you experienced with measuring folate levels in mouse plasma? If yes, which method do you usually use and what levels do you expect in a healthy adult mouse? Thanks lots for your help!
I need to know how long after ovariectomy in rodents eliminates the physiological effect of estrogens and if I recommend some paper about this.
I am working on a behavioral and molecular study in a transgenic mice model. I want to evaluate the improvement in cognitive ability among mice after using certain agents. I want to know if it is necessary to test Morris maze among both male and female?
Please, if possible provide me with an article to read more about this.
I intend to use the Callaway approach with (EnvA-deltaG) rabies virus for monosynaptic retrograde tracing. My idea is to inject, at the same time as the rabies, the 'classical' retrograde tracer Fast Blue, as a kind of positive control. Does anyone have experience with that?? I'm worried that Fast Blue might somehow disturb the action of the rabies virus..
I'm working on intestinal protozoans of poultry. I have an objective to estimate the impact of damage of parasites on the productivity of the poultry I'm working with. I am not completely sure I know what parameters to measure and how to measure them. Thanks.
While working in Ghana, I came across a number of severely abnormal marine turtle hatchlings, including ones with only one eye (in the middle of the head), deformed heads (skull deformations), deformed spines, and co-joined twins.
It seems very odd that this was common on one beach, but not on any other beach I've worked on.
How to distinguish between the scats of Cats ( Leopard, clouded leopards, jungle cat, marbled cat, leopard cat), civets (small and large indian civets) and canids (Dhole, golden jackel)?
I am currently working with triton x-100 and i was just wondering whether I could make a 10% stock solution of triton x-100 to induce hyperlipidemia in rats. another one is that I don't unerstand how i can make a 200 mg/kg dosage of this solution if it is in liquid form.
I would like this information, preferably from reliable data collection, in order to carry out FAR dung survey methods to estimate camel populations in Dhofar, Oman. It could save me a lot of time!
I am recently doing experiments on CA1 pyramidal neurons from adult mice to induce the GABA tonic current by bicuculline in the presence of TTX (0.2uM), APV (50uM) and CNQX (20uM), but the tonic current is always downward not upward as previously reported. Does anyone have same experience or any suggestion. I excluded the contamination of bicucullline.
Birkhead in his book "Bird Sense" (2012) writes that unfortunately there is very little published about touch receptors in the bills of small birds.
He also refers to the work of Herman Berkhoudt (1980, 1985) who has studied this issue and who found several touch receptors including Merkel cell receptors, double-column Merkel cell receptors and many Herbst corpuscles, all those in a zebra finch bill (Birkhead 2012). Other species beaks may contain even more receptors. I also tend to believe that both cormorants and pelicans may well have lots of such yet unstudied receptors in their beaks or pouches or other parts of their front bodies which help them locate prey. Unfortunately, I suppose there is no or very little research done in such anatomical and physiological issues on birds right now, so we may discover them after many years.
I would like to know the precise caloric value of the average daily food intake for an adult vervet monkey (or cercopithecines). I would like to study them in the wild but I haven't been successful so far in finding literature on the species caloric value for an average day.
Animals have mitochondial where oxidative phosphorylation can occur but what happens in bacteria; how do they carry out this process?
I was told that females of Atlantic Spotted Dolphin tend to get more spots after going through pregnancy, due to hormonal variation, in the same way some women notice changes in pigmentation during pregnancy.
Any opinions on this?
I´ve been looking for papers regarding this topic but found none myself...
I just found a tooth in the zygomatic arch in a marsupial skull, I have never seen that before. I want to know if anyone already seen that too.
It is known that women have higher brain perfusion than men, but I'm strugling on finding reportes about mice in physiological conditions. I'm using 8-10 month mice and DCE-MRI, but evidence from any other technique or rodent would be of great value. Thanks in advance!
I want to knwo I if there is an assay kit that is easy to be used and specific for quantifying the levels of different cytokines in the blood of dogs.
I want to work on pesticides and heavy metal contamination in egret, which is a least concerned bird in red data book, but is an important bird in agricultural system.
i need 4-5 ml blood from this bird and some eggs (4-5). what are the laws that i shall follow before perusing my experiment.
if i need to take permission from some government agency or some thing like that?
The calcium-sensing receptors (CaSR), are receptors senses levels of calcium ion in the human body, where are they located? blood vessels? parathyroid gland? or where?
I need know the mechanism of action BD1063. Is antagonist of sigma 1 receptor but I can't find a paper whit mechanism.
Thanks for your time.
I'm working on snail project but there is some numbers I just can't find such as the substitution rate, mortality rate, and fertility rate. I need those rates to plan the total number of animals.
The specie is the Helix Aspersa Maxima.
If anyone knows something about this species, please, share! Thanks
I have recorded meerkats in zoo, and need exact discrimination of call types, becacuse i cant sort them from behavioural context. I will be glad if someone cant help me. I can send wave files of calls. Thanks!
I would like to use Spike2 to compare evoked potentials from two signals recorded with a Neuralynx Cheetah Data Acquisition System with different Input Range values. As I need to compare the amplitude of the responses I am not sure if I need to apply some correction in the voltage scale or if the input range does not affect these values.
Thank you very much in advance!
I need to estimate (with power calculations) how many animals would be needed for my experiments with primary mouse hepatocytes. Assuming good yeilds.
If using large fish, I can use its blood to assess osmolality, electrolyte content, stress indicator (such as blood glucose), hormone, etc, what if i use small fish? is whole body measurement reliable enough?
Dose anybody know the collagen level in the testis of animals, specifically mice? And if there is any difference in the level between human and mice testis?