Questions related to Animal Nutrition
How this system works, how to apply, what kind of terms should be provide to suit the system? Thank you
milk, baby formula has an expiration date beyond which it is unfit for human consumption. Manufacturers, who must discard millions of pounds of outdated formula annually
I have run a western blot multiple times on the same samples and have gotten significant bands with a casein antibody in the wrong place. The molecular weight of b-casein is around 24 kDa, however my band is appearing around 125 kDa. I've tried to research if casein is often glycosylated or forms any dimers but I haven't had any success finding much. Can anyone help?
Here is the only thing I found so far that seems helpful.
"Modification of milk proteins by transglutaminase leads to their partial cross-linking and formation of high molecular weight polymers in the range of 55–200 kDa" from link:
Why would the beta-casein antibody detect milk proteins modified by transglutaminase?
Further, does this mean that casein is really in the samples. I assume yes since the band is strong.
We have had a few cases of mandibular osteodystrophy in adult dromedary and want to be sure we are balancing their ration properly. The basis of their diet is grass and home grown grass hay in Pennsylvania, USA supplemented with alpaca feed. We would like to know the recommended levels of Ca, P, Na, Cl, Vit D (and other nutrients to make a concentrated feed that will balance the nutrients provided in the feed)
We have a variety of Africa browse species for which we have forage nutrition data (NDF, ADF, mADF, ADL, CP, OM, ash). Boscia, Acacia, and Terminalia are just some of the species we've sampled.
We wish to estimate the energy and or digestibility of those samples, based on our nutritional data. Plenty of equations exist out there, however, such equations (e.g. Givens) are generally derived from grasses and not necessarily applicable to browse species.
If anyone knows of any browse specific equations or have any advice or ideas, it would be greatly appreciated.
I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker.
Does anyone know of a type of marker that can be used like this in Nile tilapia?
I will be quantifying amino acids from blood plasma via HPLC. I am planning to use leucine as internal standard. Is it already sufficient to profile all (20) amino acids or should I have more than one internal standard?
Methane production in ruminant had been reported to vary according to diet type and quality, its intake etc.
Can any one help me to put nutritional plan to produce at least 12 liter milk / day (8% fat) for at least 280 day with Egyptian buffalo?
We have 6 steers that we are considering re-vamping the diet for. Currently we get TMR from a local dairy farm which requires sending a person for 1 day twice a week to pick it up. We would like to make our own but it would require being able to store corn silage on site. I don't know of an easy way to store and feed such small quantities of corn silage. We would be feeding out maybe 100lbs of corn silage per day. Commercial storage options aren't small enough - the silage would spoil too quickly. Maybe some of you have dealt with this issue in research trials - did you find an easy way to store and feed the silage to a small number of animals? Thank you.
Please share the materials and views on canine and feline feeding. In recent times owners prefer to consult doctors more frequently regarding dog foods, quantity to be offered ingredients etc. And many myths are also associated like salt feeding sugar feeding even people dont like to offer milk to dogs. Please put some light and share your experiences in pet feeding.
Please suggest a balanced feedung schedule for dogs and cats.?
Thanks and regards
In China, India and Indonesia, where the bulk of aquaculture, globally, is practiced, supplementary feeding is a major feeding practice.
I have certain substances (complex macronutrients with minerals and vitamins) that would be registered as a supplement for avoiding stunting in humans. I have planned to make stunting offspring in the animal study but I have no idea with the procedure. Can I do that? what are the consideration and parameters so that it can be approved ethically?
thanks in advance for your answer
A lot of factors can be related to litter quality such as, intestinal health, pododermatitis and growth performances. The question is how and if there was more factors which can be related to characteristics of litter.
I would like to calculate the FCR for a diet-induced obesity experiment in rats. Since I have many nutrients/groups, I am calculating the food intake (FI) in kcal, not in grams of food. Has anybody ever calculated the FCR in kcal or knows a valid formula for it? I am not sure whether estimating the FCR as kcal/g of weight gain would be correct, since both the FI and the weight gain need to be in similar units. Some of the works I have been reading are not discerning between foods, and estimate the FCR as FI (g) / weight gain (g).
Many thanks in advance!
I recently read an article regarding the use of melatonin as a neuroprotective agent using acute ethanol exposure for 1 day and it had good results, I want to know if exists an article using functional food pre ethanol treatment
Gut microbes ferment carbohydrates and produce SCFA among other products. They can also feed on bile acids.
Are they also able to use fatty acids and triglycerides as sources of energy?
Thank you to the expert scientists that can help this uneducated PhD who is too busy to dive into literature at the moment.
I am currently trying out different binders that works well with molasses and/or CMS. So far I've chosen 1-2% sodium bentonite, 1-2%sodium lignosulfonate, and 1-2% guar meal. I was wondering if there is anything else I should try and what concentration should I use?
Oils, particularly palm and coco oils, are essentially regarded in terms of quality based on its Free Fatty Acid (FFA) Value, Iodine Number, and Peroxides Value.
What is the relationship between these three parameters? Does it affect one another? How?
What are the factors that may cause the change of value of each parameter, specifically during storage in bulk tanks?
In animal nutrition, specially for chickens and swines, what are the effects of the quantity of each parameter present in an animal feed?
I know that some nutritional deficiency, entric diseases or viral infections could result in pica in chickens, but what is the most probable reason if it was not related to nutrition? and what we can do for these chickens?
i am planning to do some research work on malnutrition model of rat, so i want to create a general malnutrition model on rat. for this can i develop the malnutrition model of rat by providing half the amount of normal diet given to the normal group of animal ?
It is known that higher tannin concentrations can be harmful or even toxic to animals. What happens when the Tannin-protein bounds are being loosened in the abomasum due to low ph influence? The tannins will be released and thus getting back their activity potential in the duodenum and Ileum, damaging the mucosa ... or is the tannin level too low for creating serious gut problems?
Hi, I'm doing my research about quail feed with micro-algae as feed additive(spirulina and chlorella). I'm using 7 day old quail as a subject until it become 35 day old. So, does anyone can help me with the reference of the correlation of growth in quail with fatty acid level in quail meat
which countries in europe are the exporting?
which countries in europe already consuming insects for human use?
which countries are already using insects for animal feed?
how much protein can be supplied by insects in broiler feed?
Tell me your views in this sense for both dairy and beef cattle.
I am currently designing a project to determine the diet of Bell Miners, Manorina melanophrys, and I am wondering whether anyone can recommend the simplest and most effective way of sampling insectivorous bird diet. To make it complicated, they also feed on psyllid lerp, the sugary coating produced by a herbivorous insect.
Looking for association between Mg intake and various psychological factors.
If we have the following fatty acid composition of C16:0, 80%, C18:0, 5%, C18:1, 10%, C18:2, 5%, in the form of triglycerides and in the form of free fatty acid. Which form will give higher digestibility as an animal feed?.
In other words, if we fed animals with free fatty acids instead of triglycerides, does this has a negative impact?.
Because treated bio-solid amount appear to be a big problem, what is its effect on forages and fodder shrubs yield and on livestock (sheep and goat). Is their any references? Thanks.
Food and protozoal N and residual N are normally calculated while knowing the effect of different feeds on rumen fermentation patterns
I want to understand if the existence and diversity of the strains within GIT microbiota in monogastrics is affected by genetic factors?
this is really a great study pertaining to animal nutrition at farmers level. but my doubt is, which will be better improvement of roughage quality by processing or improvement of rumen parameters. i feel combination of both may be even better?. and is it applicable at farmers level.
what are the possible economics?
please keep us updated as this relates to grass root level research. all the best
I am studying about digestibility of animals by using chromic oxide (Cr2O3) as an indicator in their diet and feces. To find the amount chromium in the diet and feces samples by FAAS, The amount of Cr2O3 was approximately 2-5 mg/g. I prepared the samples using acid digestion as described by EPA 3050B (https://www.epa.gov/sites/production/files/2015-06/.../epa-3050b.pdf) in order to make Cr in the samples to be soluble form. After the samples were digested with conc.HNO3, 30%H2O2, and conc.HCl, respectively, I got pale green suspended particles in some digested sample solutions after they were cooled to room temperature. Is it usual for this method? What is the green particle? Did the Cr turn to be solid form?
What is the best way to measure fecal ETEC shedding in pigs after ETEC challenge from time-effective and result accurate standpoint?
How do I collect and store samples, eg, can I collect fresh fecals on the floor or has to be directly from rectum? Can I store the fecals at -20?
i wan to know if the farming system have any effect on the worm load in sheep and goats. i want to know if the farming system have any direct link with albendazole resistance in small ruminants
The cyanogenic glycosides from different strains of sweet and bitter cassava tubers will be extracted and quantified. Graded doses of the extract would be added to the fly diet to assess their potential toxicity.
I've been reading a few things about adding salt to the grass silage, however, I was unsure if this addition will increase the cow's propensity for milk fever. I ask this because by adding Na (which is a cation) we are increasing the DCAB and elevated DCAB is supposedly related to the occurrence of milk fever.
I found that the grass silage has around 0.29% Na in DM and that in order to have a low risk of milk fever the Na content should be 17% in DM.
What can you say about this?
I am preparing a project of enrichment of captive Sun bear. There are two sun bears staying together in the zoo, both of them are 3 years old, one is female, another one is male. The female one sucks its hand all the time. I am wondering whether the feed is enough for them or not ( about pellet feed 1 kg per day for them, and some furits). I could not find the review paper about feed nutrition standards and feed intake of Sun bear. Is there any one could give me some suggestion? Thank you.
Hello every body
I have several articles about it. I think that there is a difference. Is this true? Please help me to understand it.
Does it make sense taking into account that humans need DHA and EPA and cows scarcely convert C18:3n3 into them?
I have body weight gain data. I need to estimate methionine requirement on the basis of maximum body weight gain obtained from this data. I need to use linear broken and quadratic broken line model to estimate methionine requirement.
We are doing an experiment where mice must eat a highly preferred food, which has been manipulated to have an unpleasant taste. We planned to use quinine but it may not arrive in time for our experiment.
Is there something else we can put on their food which will a) have no smell (so the mice will still be motivated to approach and try the food), but b) taste unpleasant to a mouse? Oh, and perhaps I should add, c) it should not kill the mice.
Thanks in advance for any ideas!
I need to feed the rat with powder high fat diet. We used to mix the powder high fat diet with coconut oil and make the powder diet into small balls. But, the rats are not eating these balls properly as compare with pellet. Is there any simple method for intragastric administration of powdered materials to rats?
Thanking you in advance for your valuable suggestions.
Drinking water, acid secretion and digestion process are inter-related. I am interested to know, when and in what quantity one should drink water. In monogastrics and ruminants what should be the rules for optimization of digestion and assimilation process. If food changes to meat based item what changed we should do and so also for fibre and concentrate ratio in ruminants. Can anyone help me on this aspect
Are these variables appropriate?
Forage species composition and biomass using remote sensing techniques.
Chemical composition (including lipids and tannins) of forages and supplements, forage: concentrate ratio, additives
Animal characteristics, including species, number, age, starting weight, weight gain, daily feed int
Excreted total nitrogen (NH4+, urine N, faecal N) and excreted volatile solids
Rumen pH and rumen microbes (quantity)
Environmental conditions, particularly, temperature and relative humidity
Methane and nitrous oxide fluxes from herds of ruminants using infra-red gas analysers
I'm looking to administer estriol over a long period of time (like more than a year). Multiple pellet implantations is not really feasible because it would be really bad for the mice. Also IP injections daily for more than a year would be tiring for me and stressful for the mice. So basically I'm curious if there is a way to continuously give the mice estriol through food or water.
In ruminants concentrates, oils inclusion is lower that in monogastrics' ones, where low durability and pasty textures can be found due to high inclusion of fats.
In a 8% oil inclusion (monogastrics), the partial substitution of oil for calcium salts of PFAD has shown to be benefitial. However, in ruminants concrentrates (i.e. 1,5% fat) there would be any possitive effect? Could you please provide with data in this sense (either with calcium soaps of fatty acids or hydrogenated fats)?
I have read numerous papers designed to determine the digestibility of whole proteins (ie fishmeal) using both external and inert markers such as chromic oxide and acid insoluble ash. However, no method that can be used to determine digestibility of amino acid supplements that are crystalline in structure. The diets will be fed to slow feeding aquatic organisms. The obvious concern here is the solubility of crystalline structures in water, and there are worries of supplemented crystalline amino acids leaching from the diet and faeces, which may skew results of digestibility. In this context could acid Insoluble ash be used as an inert marker? Are there any other methodologies to perhaps use?
I have prepared a ketogenic diet consisting of 70% fat.. I am facing problem in administering it in animals ad libitum because of its paste like consistency. Can anyone really help me solve this problem??
I am using XRF (Rigaku NEX-CG) to quantify nutritional elements in complex animal feed materials. In theory the Empirical approach to calibration (preparing your own standards by spiking a blank matrix) should be more accurate than the Matching Library approach (using wet chemistry data from real samples to adjust the sensitivity of the on-board XRF calibration). In your experience is this the case in practice? Is an Empirical calibration actually more accurate or not?
Secondly, has anyone had success in creating a single Matching Library for a range of different sample matrices? How have you defined the range of samples matrices which are included (similar ash contents? similar major constituents...)? How similar do these matrices need to be to fit within the one method? Any thoughts gratefully received.
in below link they mentioned in page 468 under the subtitle" the JECFA REVIEW"
the oral lethal dose of MSG in mouse and rat was between 10-20g/kg body weight and in this context , the palatable dose of MSG in humans approximately 60 mg/kg body weight?
please , i want to know how that approximation was done?
I noticed big ambiguity in literature regarding AMEn content of Jowar poultry. Hence please suggest me exact AMEn value that could be taken poultry ration formulation.