Questions related to Animal Dissection
I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around 0.2% in spleen but I could not find data about FRC. What is the expected frequency of FRC in mice spleen? Thanks.
I have whole mouse brains fixed in PFA that I need to microdissect specific regions: hippocampus, cerebellum, striatum,and frontal cortex for DNA methylation analysis. Is there a staining (or any other) technique I could use to verify that I have dissected the region I intended, and that there is not contamination with surrounding regions?
Actually, I want to know the possible way and procedure to sample CSF from cisterna magna of Rat brain without any surgery and opening the skin in this area.
I would like to perform immunofluorecence on human bone marrow biopsies which are snap frozen. I received bone marrow biopsies from the tibia (spongy material, 1x1 cm) which I embedded in OCT and froze in 2-methyl-butane. Unfortunately, cutting sections of this material seems to be impossible since the tissue ruptures very easily. Does someone know how to optimize this protocol and prepare the bone marrow sections?
I know that the needle size will affect disruption of the cells, but do cells still get disrupted at the larger size? TIA
I am trying to isolate total RNA from tadpoles for transcriptomics studies. I have to do for the first time so if someone has a protocol.please share with me
Hey. For osteology fish. Someone has a protocol or an approach to dissect and visualize the bone head?
I am trying to track sperm cells in the oviduct. However, I am facing some difficulties uncoiling the oviduct.I am preforming the dissection on a super-ovulated female a few hours after mating and I cannot seem to locate where to make the incision/the cut in order to remove the ovarian bursa without harming the oviduct itself.
I am working on a teleost fish from Cyprinidae family. I have read many procedures for head kidney (HK) isolation and maintaining its culture but if anyone could send me the links of videos it will be more helpful.
I'm attempting to carry out some experiments which require me to remove long stretches of intact spinal cord. I came across some literature which describes hydraulic extraction using a syringe to push the entire spinal cord out in one shot. I have tried a few times but the cord doesn't seem to come out as easily/cleanly as the literature suggests. Any advice on how to improve my extractions?
I am working on some tissues (Liver, Spleen, Pancreas) to isolate nuclei, every time I try to isolate the nuclei, the sample full of red blood cells and I can't distinguish between nuclei and RBC. Is there any way to eliminate the RBC?
I found out it's about prevention of damaging the nerve tissue, etc. from google but I would like further precise explanation for this please. Thank you.
Recently the University Grants Commission, New Delhi, issued a directive to all colleges and universities in the country to find alternatives to animal dissection and animal experimentation especially at undergraduate and post-graduate levels. I want to know if some is using such alternatives and if that could be shared?
I need to perform simultaneous hippocampal and striatal dissections, is this possible without disrupting the tissue? The mice are 12 months old. Thank you very much!
I want to micro dissect the brain of rats and mice to isolate hippocampus, prefrontal cortex and other brain parts. Can help me out to find the best video on micro dissection of brain either in youtube or any other website? Thank you.
I am trying to collect the hippocampus, prefrontal cortex and amygdala from adolescent rat brains (unfixed). Is there a way I could validate the accuracy of the dissection to rule out contamination from adjacent brain regions? For instance, I have been searching for molecular markers of the hippocampus that are absent in adjacent regions, and vice versa.
I am finding it difficult to identify the lymph node. I would like to remove then, paraffin embed and stain with H&E.
I am wondering if there is a method for dissecting adjacent normal and tumor samples from living mice without sacrificing the mouse? I hope to study the tumor evolution at multiple time points from the same mice. Is it possible?