Anesthesia - Science topic

why do some rats die after anesthesia with Ketamine/Xylazine,maintained by isoflurane?

What may be the future of Thoracic Segmental Anesthesia in healthy cohorts?

Can somebody assist me with the intranasal inhalation protocol(detailed or standardized).

I am using isoflurane for anesthesia in Swiss albino mice. However, for the last few weeks, when I give isoflurane to the mice before starting the operation, the mice became slightly dizzy (not fully anaesthetized) before placing them in stereotaxic apparatus, but they suddenly threw their necks back and contracted, and stayed in this way for about 40-45 seconds as if they were having a seizure, then returned to normal. I've never encountered this kind of problem before. Does anyone have any idea about why this is happening or have experienced this before?

I am looking for experts in the field of perioperative hypothermia for a content validation study.

I am very happy if you support me here!

The inclusion criteria are:

I would be glad if you could support me! Please contact me on research gate or via email manuel.schwanda@fhstp.ac.at

Thank you very much for your valuable support!

Warm regards!

Manuel

I have a problem about rat anesthesia, the situation is that I have Zoletil 50 1mg/ml + xylazine hydrochloride 23.32mg/ml solution that I prepare at the same time from the same source (from the same stock)

I give it to two Wistar rats that both weight 470g, IP 500ul, one rat anesthetizes without problem, but the other one rat fails to anesthetize and stay awake.

This is happening more and more often; I don’t know the reason or how to solve it.

Thank you.

I would like to ask anesthesiologists or clinicians who have experienced anesthesia mumps. Was there a case in which the swelling of the parotid gland is associated with a retrograde insufflation of air in the Stensen’s duct and the parotid gland (pneumoparotid)? If you know the literature on anesthesia mumps confirmed by CT as pneumoparotid, I would appreciate it if you could let me know.

Drugs of Vedana Sthapana Mahakashya of Charaka Samhita are having Volatile oil contain and fragrance. What will be the applicability in the light of Sangyaharan –anesthesia?

Dear community,

I was wondering if anesthesia (most likely isofluran) of animals before euthanasia and sampling of internal organs (here reproductive tracts in lizards) for RNA seq might ater the mRNA expression profile?

Would you recommand to perfor the euthanasia without the anesthesia or would you anesthetized them?

Thanks a lot for your answers,

Morgane

Do C57 mice have to be anesthetized by inhalation before removing the eye to extract blood? Does anyone know the exact anesthesia procedure and is there any relevant literature to recommend? Since the lab is not equipped with an anesthesia machine, is it possible to use the beaker inversion method for anesthesia? Or is there another easy way?

what is suitable time for preanesthesia evaluation and preparation? 

I have a survey with dichotomous variables and need tetrachoric/polycoric analysis.  I used R module but tried to run it also in SPSS and cannot do polycoric analysis (keep getting a message - could not find function "hetcor").  I checked on extensions and it is telling me that extension HETCOR is installed.  Any suggestions? I am running out of options.  Thank you so much!!!

In a study there are some animal species need to implement a coronary stent from (femoral artery) and to undergo a laparotomy in order to place a telemetric transponder.

What are the recommended anesthetic drugs that induct anesthesia without affecting the cardiovascular system at the time of procedures for equine?

I tried to perform SSEP test in rat SCI model but I had the problems in anesthesia. I used the thiopental (that we had in lab.) 30 mg/kg IP, but the rats hadn't good anesthesia or died.

What is the suitable method to anesthesia in somatosensory evoked potential test in rat spinal cord injury model?

Using eugenol to anesthetize freshwater angelfish (Pterophyllum scalare), behavior is apparently affected not only during anesthesia, but also after anesthesia.

The study by Cooke et al. (2004) is interesting.

Cooke, S.J .; Suski, C.D .; Ostranda, K.G .; Tufts, B.L .; Wahl, D.H. Behavioral and physiological assessment of low concentrations of clove oil anesthetic for handling and transporting largemouth bass (Micropterus salmoides). Aquaculture, v.239, p.509-529, 2004.

Are there other studies that have evaluated the behavior of anesthetized fish?

Is anyone able to tell me why my anaesthetized rats often do a 'double breath', or diaphragmatic breath, like a hiccup (see video). This often begins after 1-2 hours under anesthesia, and can occur every 4th to 10th breath. The rats are anaesthetized with isoflurane (usually set to 1.5L/min). They are ventilated with a mixture of O2 and air (about 30:70). The ventilator is on volume control. When the rats 'double breathe' like this, I can sometimes stop it by turning the tidal volume right up, but this then leads to severe hypocapnia. There is no obvious cause for the development of this abnormal breathing (end tidal CO2 is in the normal range and the rats are not getting light in terms of anesthesia). Can anyone help?

I am trying to start a protocol involving bilateral cannula implantation in specific brain regions in mice (mPFC, hippocampus). For that, I need to buy an anesthesia system. I found many alternative companies to buy from, but I am particularly interested in MINERVE 1301303. I am also considering WPI EZ-B800 or Kent Scientific SomnoSuite® Low-Flow Anesthesia System. Can anyone give me feedback about these anesthesia systems? Maybe you have other recommendations? I will be grateful for all suggestions.

As an anesthesiologist and also perioperative management, is more concerned with the level of blood sugar at the time or during perioperative time. If that time level is ok, then, will it have an effect in management by knowing the last three months status (even if it was poorly controlled)?

Hi there, 

I am working right now with the urethane to anesthetize mice. When I started I felt that the anesthesia effect was very little reliable, as people commented that the time until the mice were completely anesthetized would vary from 45 min to -sometimes- even 2 or more hours. Some colleagues recommended me to apply the urethane (1.5 gr/kilo) in different dosis (50%-25%-25%, 15 min apart from one to another), which I am doing, although two hours later the animal still has reflexes. I have read studies were the urethane and ketamine are combined. Aproximately 1 hour and a half after the first urethane injection, ketamine is injected to start the surgery, but the problem is that there is nothing written for mice (just rats, at least that I know) and I am struggling because depending on the animal it goes ok, or bad or it has breathing problems only 30 min later (I perform tracheotomy by the way, so that it is why I want the animal to lose the reflexes as soon as possible); is it just me? So, I was wondering if anybody out there was working in a reliable way with urethane in mice. I.e., the dosis of ketamine you apply after the first dosis of urethane, how long after, how long does it take to anesthetize the animal completely, etc.. according your experience? 

I would really appreciate your help.

Thanks!

Hi,

Could we use the chloroform to anesthesia the mices before them euthanasia according to the ethical rules of experiments.

Best regards.

What is the roles If we want to use frequent Intrathecal administration for rat model (treatment)?

Especially that we need to inject the rats for 7 days under anesthesia?

Please include reference to your answer.

Thanks

I am looking for EEG dataset for anesthesia, please where can I find such dataset?

What are the best ways of anesthesia and euthanasia in laboratory rabbits?

What are the best ways of anesthesia and euthanasia in laboratory rabbits and other rodents?

I would like to ask if it's possible to use BIS Quatro Sensor together with a standard 64-channel EEG Cap? What would be the recommended montage in that case?

I don't seem to be able to find any complete and fully-featured methodology for dissection of holothuria in order to be able to isolate organs and tissues efficiently. I actually would also like more info regarding the process of anesthesia so that a clean incision may be possible.

Thank you in advance!!

The American Society of Anesthesiologists physical status classification has been modified a few times to increase the uniformity. The recent amendment was done in 2014 which has also incorporated some examples against each class. Still, sometimes I feel difficulty in assigning the class especially patients having a history of heavy smoking in the past, tobacco users, cancer patients, patients having multiple controlled or mild co-morbidities, etc. Do you also face the same situation? If so, in which cases?

The patient should consult which specialty if he wants to know, will he stop aspirin prior to the operation or not? Will he consult the Cardiologist, the surgeon or the anesthesiologist?

Several studies have shown that occurrence of Burst Suppression is associated with cognitive complications after surgery and anesthesia and sedation during Intensive Care Management. Recente studies present contradictory evidence...

But we know that BS is not a normal EEG pattern so... ??

Awake craniotomy and related anesthetic techniques in the operating theatre

Closed loop ansthesia delivery systems typically use TIVA, using propofol or other intra venous drugs. I want to now if there is any system which use inhalational anesthetics to alter the plane of anesthesia .

i want to observe an outcome difference between the two hand of the same patient. one hand is exposed to a routine intra-operative intervention while the other hand is not. i expect this outcome intra- operatively under anesthesia, and will a prospective cohort study be suitable to study the difference in outcome between the two hands during this short period of time, and if not, what is the more suitable type of observational study

Losing consciousness is always associated with concern of privacy. People often fear more of anesthesia than surgery, which is the primary objective. Anesthetists strive to allay anxiety & make people assured & happy. Share your experiences if you've ever encountered it, please.

Beyond the events on October 16th, 1846 ("Ether Day"), which stories are worth to be recognised about the history of Anesthesiology? Which are the most impressive, curious, or funny things we should remember?

In my postdoc I always used the anesthesic protocol of ketamine(80mg/kg)/xylazine (10mg/kg) injecting 0,1ml ip for balb-c mice. And it works very well.

However now using Swiss mice from another breeding this protocol is not working. We tryed a higher dose of 100mg/kg ketamine and 16mg/kg of xylazine but stil not working.

Did someone have another ketamine/xylazine protocol for swiss mice anesthesia? (I only have access of this drugs).

Thanks

54 Y Female who will undergo vaginal hysterectomy along with some issues in the anal canal and bladder.

Medical History:

Atrial Fibrillation (Managed by Bisoprolol 2.5 mg once daily)

Chronic venous insufficiency (Managed by Daflon one tablet once daily)

Allergic Rhinitis (Managed by Cetirizine 10 mg once daily)

Non alcoholic fatty liver disease

Past (maybe relevant history) - She went 2 years ago to the ICU because she lost abnormal amounts of blood along with AFib episode which required blood transfusion, during her stay in the ICU, some symptoms and abnormalities occurred like Fever and Superficial thrombophlebitis.

Beside this, some signs and symptoms that the cardiologist agreed not to investigate the cause and there is no need to (After he investigated the Echocardiogram, Blood pressure, ECG : Occassional dyspnea, some symptoms that mimic coronary artery disease like Chest pain.

The Anesthesiologist plan according to this history and CBC and some questions that I can post answers to if you asked them:

General Anesthesia and she shouldn't discontinue Daflon and should take Enoxaparin two days consequently prior to the surgery.

My Q is, is prophylaxis using Enoxaparin considered to be good? Also what about Daflon, should she continue using it? Final one, does she need to stop dietary garlic 7 days prior to the surgery because the Anesthesiologist forgot to mention this one ?

I wanted to induce bacterila infection to the mmice and we have alwaays performed it in normal conditions by restraining the mouse but would it make a difference when we give small dose of isoflurane and then inject via tail vein?

what type of anesthesia you do for patients with CSDH? Are you interested to contribute with an RCT for awake vs GA in patients with CSDH?

what type of anesthesia you do for patients with CSDH? Are you interested to contribute with an RCT for awake vs GA in patients with CSDH?

what type of anesthesia you do for patients with CSDH? Are you interested to contribute with an RCT for awake vs GA in patients with CSDH?

In spite of the metaanalysis (Does central venous pressure predict fluid responsiveness? A systematic review of the literature and the tale of seven mares). Published in the

Chest. 2008 Jul;134(1):172-8.

Marik PE, Baram M, Vahid B, with the Conclusion:

(This systematic review demonstrated a very poor relationship between CVP and blood volume as well as the inability of CVP/DeltaCVP to predict the hemodynamic response to a fluid challenge. CVP should not be used to make clinical decisions regarding fluid management.) CVP may still the most widely used monitor for fluid management worldwide, do think that is true? Do you think it is accepted practice? And why?

In the view, use of ultrasound has improved regional anesthesia technique, RA could be choice of Anesthesia for various surgical procedure!

General Anesthesia is ofcourse safe way to proctect airway.

What is current opinions for choice of anesthesia when NPO status is not adequate? GA vs RA?

In sleep, brain activity, specially in the default mode network (DMN) is reduced. For example, Amyloid beta production by neurons has a 70 % jump in wakefulness compared with sleep (Kress et al. 2018 J Exper Med). Can one assume that the same would happen in case of coma, or anaesthesia? Are they similar at all in terms of brain activity?

As I searched, it seems we are able to induce an artificial coma, like what we do for anaesthesia? If so, how long could it last at most?

The last but not least, have people taken effect systematic MRI during comma, anaesthesia, and sleep?

I thank you in advance for your help.

My group and I have developed a pre-anesthesia health assessment screening questionnaire. We would like to validate if the questionnaire can be administered by patients on their own before their interview with anesthetist. The gold standard method at the clinic involves the nurse anesthetist interviewing the patient using a questionnaire. The literature describes two methods: (1) the anesthesia caregiver interviews the patient but is blinded to the responses provided by the patient; (2) the anesthetist checks the patient's responses during the interview by reading out the question and confirming the answer. The published studies did not explain why the methods described above were used. For a questionnaire validation study, which method would be appropriate? Which would yield the least biased assessment and what advantages and disadvantages would it have? Which would be the most ethical?

Links to articles:

I am wondering if there is an article showing that the Modified Aldrete Scale is valid for use in children after anesthesia.

Though there are several animal studies but detailed method of anesthesia was not mentioned. So I need a method with proper reference.

I tried to find GAPDH from mice brain after anesthesia, Using Kit, I find RNA and then cDNA. However, I get the band but the thickness is not like that what I desired. So what to do to get a thick band.

Transesophageal echocardiography for mitral regurgitation

My animals are rat , p15-p20

Sometime it has been seen that surgical team approach the patient party / attendant for a radical procedure (for example - consent taken and planned for cystectomy and introp surgeon decided to do hysterectomy) in an general anaesthetized patient. Is it valid or acceptable? The operation is elective, patient is otherwise capable to give consent when awake and consent; taking consent from attendant / party isn't against patients autonomy?

What is the best way to anaesthetise small freshwater fishes (e.g. Gambusia) for quick recovery and minimum stress?

Ice water/ clove oil/ anything else?

Is there anyone who knows/or has made/written/done, and validated, a questionnaire for follow-up phone calls to parents of children who have undergone anesthesia in day surgery?

I think no other person knows a surgeon better than an anaesthesiologist as a professional especially about the surgical skill and quality. (because they closely observe different surgeons of same and different specialty). Many a time even a highly qualified surgeon is very poor in skill and delivers very poor for the patient. (The same may be true for anaesthesiologist too). This in turn leads to unwanted morbidity and even mortality. Anaesthesiologist is equally or may be more responsible for the well being of the patient during perioperative and especially intraoperative period. So, if the anaesthesiologist knows that the surgeon supposed to do the case is not good enough for the proposed surgery, can anaesthesiologist refuse to give (anaesthetize) the case?

When Stanley Malamed, in his books and many of his papers, writes that " IANB has the highest failure rate among all local anesthetic blocks in Medicine - not only in dental local anesthesia : are there any data in the medical literature backing this assertion, or do you think it is simply a fact that he personnaly observed or experienced as a dentist-anesthesiologist, without scientific evidence ? In the first case, are there references readily availa and if so, could anybody  let me know how and where I can get it ?

Thank you for your comments.

Is genomics in Anesthesia practice evolving? When will genomic testing be part of the pre-anesthesia work up?

Simon Body published an interesting paper in 2009 but I haven't seen the conversation continue. I'm wondering if anyone in the community is working in this area.

Standard precautions are meant to reduce the risk of transmission of bloodborne and other pathogens from both recognized and unrecognized sources. (WHO) It is also recommended to assume that every person is potentially infected or colonized with an organism that could be transmitted in the health-care setting and apply the following infection control practices during the delivery of health care. (Guideline recommendation). Personal Protective equipment are to be used as one such measure. But I am unable to find whether the OT table and floor should also be covered with plastic? Please give your opinion with logic (reasoning) and evidences.

Reference: http://www.who.int/csr/resources/publications/EPR_AM2_E7.pdf

https://www.ncbi.nlm.nih.gov/books/NBK305277/

Cost is always a concern in the present health care delivery, even in developed countries. The prevalence of such disease is quite low (in most of the area of the world). In such scenario, doing these tests in all patients costs billion. Is this cost-effective? if it should be done mandatorily, why? Or, should these tests be done based on history and examination?

Cost-effective yet quality health care delivery is one important objective worldwide now. Routine testing costs billion but without much impact. If a patient is not having pallore preop, planned for intermediate surgery; is it justified that only to know MABL and be prepared for unexpected hemorrhage, we should do preop Hb level? Hb level can even be done in point of care facility in such unexpected situations to decide transfusion...so, will it be a deficit if Hb not done in preop?

Research shows that medic legal apprehension is a big factor contributing towards the continued practice of routine preoperative testing. [doi:10.4103/ija.IJA_92_17] Whereas it is very much evident that routine preoperative laboratory testing is unnecessary and is not recommended.

Yes, urethane can induce a long lasting and deep level of anesthesia, but my problem is that it take a long time to induce the anesthesia, even if I use the dose 1,2 g/kg IP for rats. Does anyone have experience with this problem and maybe could help or give advice?

Thank you everyone for your answers!

8-16 mg codeine po q8h and 20 mg IM lidocaine HCl : are they absolutely contraindicated for patients with:

-hypertension

-arrhythmias with pacemaker. 

Or they can be given under medical advice in certain conditions?

i need 15 minutes of the animal in anesthetic condition prescribed dose of  ketamine is -80 mg/kg and for xylazine 10 mg/kg, why we can not use either ketamine or xylazine,  plz let me know ?

Non responsive geriatrics's pt after cardiac surgery-not waking from anesthesia .

Advise/article to assist in the waking of a 79 petite woman who underwent cardiac redo surgery of mitral valve over a week ago and has not woken from duty. Suffered renal damage from surgery and creating high at 2.7. Eeg and ct done nothing significant, neuro eval insignificant.  On fentanyl and ketamine- introducing  Seraquil. Pt flayles and is agitated and secured to bed but non responsive-any advise? Thanks 

i intent to know whether anesthesia is necessary or not. also what type of capillaries are best for the procedure. 

Dear Colleagues,

During electropysiological recordings from rat brain we observe that Evoked LFP responses can change based on anesthesia depth over time. Even if you keep the vaporizer flow at the same level, the anesthesia effect will change in time, decrease or increase and it will directly  effect  the recordings.

1) I need to, non-invasively, measure anesthesia depth, what do you think would be the best? 

This is what I know:

       Since we are recording LFP signals, we can make an estimate from LFP activities but I am not sure how to use LFPs for this. Would   measuring the frequency UP and DOWN states would be enough?

      Some people say EEG is best but I we dont want to use this if possible, our setup has some restriction for this.

      Heart rate and blood pressure could be good but which sensors  would be ideal?  We would connect the output of the sensor to our recording board for continuous recording.

Any help with references or from personal experience would be appreciated. 

Thank you

what the causes  that prevent from uses the local in this animal

We are doing survival surgery on the spinal cords of Sprague-Dawley rats but our animals are not surviving after surgery. We are using isoflurane for 5 minutes in the beginning to induce and then using an I.P injection of a ketamine/xylazine cocktail at 90mg/kg ketamine and 4mg/kg xylazine. We then move them to a cage with a heat lamp. We never had this issue before but now a majority of our rats are dying after the surgery is complete and we don't what we can adjust to keep them alive. 

Dear colleagues. I am interested in knowing methods and appares for measuring intraocular pressure using transportable and reliable devices that do not require anesthesia or connection to electric current. Someone could tell me, based on your experience, models or references of tonometers that meet these characteristics. Thank you very much in advance.

Good morning,

I would like to know which cocktail of anaesthesia do you use for the transgenic line 5xFAD Tg6799. We have tried to use a cocktail of ketamine/rompun but specially female do not perform well and males have sometimes epileptic seizures.

We do not have the infrastructure to use isofluorane, so we won't use it.

Any help is welcome. Thank you.

Ultrasound has been used for different functions, what is its role in the diagnosis, evaluation and evolution of intra-abdominal hypertension?

Noticing that the incidence of PONV in Laparoscopic gastric bypass and sleeve gastrectomy patients appears higher than other laparoscopic and gynecology cases. Has anyone else noticed this phenomenon? What are you using for prophylaxis?

I am looking for software (free or paid) that allows monitoring data to be extracted from the GE Carescape B850 anesthetic monitor. Ideally the data should be downloadable in a CSV or Excel file.

ETT size

Would it be bispectral index or exhausted anaesthetic gas from CPB circuit (analogue to end tidal anaesthetic gas)?

Many thanks for your time and atttention,

Ka

Is there any well documented study(ies) supporting the statement by John Meechan in his book “Practical dental anesthesia” that epinephrine added to dental local anesthetic formulations modifies the distribution of blood in the body and sends relatively more to the brain, so that epinephrine might increase the toxicity of local anesthetic molecules on the Central Nervous System ?