Ancient DNA Analysis - Science topic

RG

Could this 2mm Ancient diamondized embryo be Martian or remnants of a lost planet .

Ancient human related or could this be some type of Reptilian.

Ancient earth /Reptilian/ ET?

John Huntley and myself are currently guest editing two volumes of Topics in Geobiology on the evolution and fossil record of parasitism. We are aware of the importance of paleoproteomics, particularly aDNA, to reveal the evolution and extinction of ancient parasites. We are having trouble finding a researcher who might be willing to contribute a review chapter on this topic this year.

We would like a contribution highlighting the importance of paleoproteomics as a technique to reveal ancient parasitism, parasite-host evolution and extinction – key would be to review the potential of these techniques (aDNA, proteins, antigen tests, microbiome) to infer constraints on parasites evolution, host pathology/infection and highlight differences with other methods (investigations of fossil propagules in coprolites, etc.).

Please sent us a private message or e-mail with a potential outline or potential contributors.

This question concerns an ancient object of art. Could DNA analysis help giving an answer?

Even I have tried several kits and protocols, I almost always fail to extract DNA from the museal specimens (ca 10-20 years old) of beetles. I would need to amplify commonly used fragments like COI, 16S, 18S but failed. Does anybody have a good experience with this? If yes, please could you provide me with suggestions and protocols? Thanks   

Does anyone knows a reliable way for extracting DNA from historical specimens in bats?

I used DNeasy (Qiagen) and invitrogen DNA mini extraction kits with digestion modification, but I am not getting DNA's. So please anyone can give me the protocol or kit to extract DNA from 50 to 100 years old museum preserved/dried bird samples. Thanking you.

I'm looking for aDNA studies on the phylogeny of European bison (Bison bonasus). Preferably both mtDNA and Y-chromosome studies. Does anyone know of such studies having been published?

I have fossil ostrich eggshells from Upper Paleolithic sites of India, I am interested in Ancient DNA analysis from these eggshells. Is anyone interested in this collaborative project?

I have an .arp file containing around 22,000 mtDNA sequences, the sequences are 244bp in length. I have been trying to compute 'pairwise FST's' and the 'number of shared haplotypes' but this has not worked. 

Prior to this analysis I have rigorously screened the file for potential problems using the Arelquin log file as a guide. The .arp file is ready for analysis and WILL compute standard and molecular diversity indices (Intra-population analysis) but FAILS to compute the FST's and Number of shared haplotypes (Inter-population analysis). The log file states that there is not enough memory although this is unlikely to be the case for a number of reasons: a) the computer being used for the analysis has 128GB of RAM and over 10TB of hard drive space b) The amount of RAM allocated to each program for use is 128GB c) the sequences are only 244bp each in length.

I tried removing the structure in the .arp file to see whether this would help but it still failed. I then tried removing around 7000 sequences. This did work and the FST's computed.

The only thing would explain why the initial analysis did not work is that Arelquin has a limitation on the number of sequences/haplotypes that can be analysed at one time, although this is not stated in the Arlequin manual. The manual states 'the amount of data that Arlequin can handle mostly depends on the memory available on your computer'. 

So I am wondering whether anyone has had problems like this before? and how did you overcome it?

Thanks in advance.

I've been using genomic screen tape on our agilent tapestation to compare DNA length in different samples, but it's really inconsistent. I get wildly different results for the same sample. I was chatting to a guy at a conference and he said he was having the same trouble. Does anyone else have experience of this, or can suggest what's going wrong?

Is the spotting through histochemical stains is diagenetic?