Science topics: MedicinePathologyAnatomic Pathology
Anatomic Pathology - Science topic
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Questions related to Anatomic Pathology
I'd like to determine microvessel dimensions in various tumor groups and am looking for an online database to draw pathology slides from.
I work with bat's helminths taxonomy. The ideal is to work on fresh hosts, but some bats species are highly threatened and it's not possible to obtain fresh carcasses or other alternatives. For these cases, I'm thinking about recovering the parasites from bats in biological collections. However the helminths recovering is difficult because of the dehydration and stiffening of the viscera when stored in formaldehyde or alcohol solutions. I've been trying to store some samples in water for some hours or days, but the visceras didn't rehydrate at all. Does anyone have some tips?
The HE stain we use is very unstable. When a slide is stained in the morning and the same slide (at a deeper cut) is stained in the afternoon there is a major difference in the color. The one from the afternoon is much lighter than the one from the morning. Please help?
Can biofilms be seen by pathologists when they do lab studies? For example, if a human has a biofilm in their colon and does a colonoscopy with biopsies sent to the pathologist, could the doctor doing the scope and/or the pathologist reviewing the biopsy actually be able to identify the biofilm? Or is the biofilm too microscopic for even today's advanced imaging.
I have a question for unbiased quantification method for tumor section staining.
I am currently doing TUNEL assay on primary breast tumor (Triple negative breast cancers) sections from mice. I have notice that the quantification data is very sensitive to where I take an image of.
In my case, the size of primary tumors were more than 400mm3 when they were collected. And all the tumors, regardless of sham or treatment groups, had varying degrees of necrotic or apoptotic core, which may be attributed to restricted oxygen and nutrition transport?
1.I assume that you should exclude those apoptotic or necrotic core tissues? Then, can I assume that event of necrotic or apoptotic core in primary cancer is completely independent of treatment (chemotherapy)?
2. For TUNEL assay, I observe the TUNEL staining of necrotic tissues that does not colocalize with DAPI staining. And these necrotic tissues, outside of core necrotic tissue, are more frequently observed in drug -treatment group. Would you still consider including those regions without DAPI colocalization?
If anybody give me general guideline for quantification of TUNEL staining or IHC staining, it would be great. I would like to get an experts opinion and be consistent and unbiased in quantification of primary tumor tissue staining.
What could we change in preparing tissue samples prior cryosections(cryostat) method for more convenience and results? ( I mean cryo-embedding) The second question is in which areas of research do you use this one and why is it a pretty rare method of imaging? It is interesting to get answers from researchers who are working currently with this method or those who worked with that recently.
Thank All in advance for any ideas.
Hello, I am currently researching a spectral computed tomography system for medical applications. The system excels in delineating the elemental composition of materials, providing a 3D representation of the each element's distribution. This has shown excellent results in determining the composition of calcifications but I'm wondering what other diseases researchers are aware of that might benefit from this technique.
I incidentally discovered intratumoral foci of extramedullary hematopoiesis in extrapleural solitary fibrous tumor recently. It seems to be exceedingly rare (2 cases reported to date?) according to my brief literature search. Is this truly rare or just under-reported?
Having worked in the Africa, I woke up late to publishing because I did not have money to process histological/histopathological specimens. I had to force myself to start with gross anatomical/pathological manuscripts. I have succeeded to some extent but lack of sophisticated techniques such as serology has seriously curtailed my work.
All I can seem to find is that DQ, DP and DR are involved in antigen presentation, whilst DM and DO are involved in peptide loading. And it seems that DR is found in equal numbers to DQ in the human intestine. Therefore, I don't really understand why you would choose to stain for DR instead of DQ?
Seem that a lack of common standards about studies of Nanoparticles cytotoxicity and others nanomateriais for interaction with living cells has been one of the limitations of the current research, mainly in neurosciences, which invoves diifferent cell lines, exposure times, and colorimetric assays that usec in different studies. For this reason, I think that it is necessary we beginning a discussion about this theme so that will be possible to compare cytotoxic effect among these results, such as single standard meets all conditions for obtaining information on nanomaterials cytotoxicity. What do you say about this theme?
I want to study human serum properties in the absence of albumin. In order to do that i need to use a protocol that will deplete most(if not all) of albumin from my serum samples but in the same time, leave rest of serum "intact" in order to be used for further experiments.
I tried to use a protocol with NaCl and Et-OH (95%) precipitation but it doesn't seem to work for me.
Anyone can help me? :)
I would like to use a vibratome in order to cut fresh (and not fixed!) heart tissue. I need the heart to be "alive" for my experiment.
Has anyone tried this option? In which buffer do I need to use it?
Many articles have been written concerning the presence of the golden number or the numbers of Fibonacci in architecture or botanical sciences. Are there any publications in the field of human anatomy or histology? Thank you!
The intent is to capture moderately cellular CSF in paraffin-embedded tissue to perform immunohistochemistry or in-situ hybridization.
I want to do cryosections of pancreas, but the tissue is really troublesome. I'm giving the animal (mouse) an injection with a hormone and following harvesting the pancreas. Before the harvest the pancreas is perfused with saline to remove the blood and any trace of unbound hormone. I've tried to snap freeze it in Isopentane and embeddet in OCT and in PFA 4% overnight followed by 30% succrose before embedding. But my sections looks like crap. I've cut them at -22 - -16 degrees.
I am trying to stain tangles in human brain frozen sections. Thioflavin T has been widely used by the people in paraffin sections, however in frozen section I am seeing lots of non specific binding. The image lights up like Christmas. Any suggestion would be appreciated.
I tried 0.5% and 0.1 % Thioflavin in 0.1N HCl.
I have only got access to certain cell lines and need to find out which CD markers they express to be able to order certain labels for testing.
....like intestine, cervic, pullmo etc.? And, can anyone suggest some references to help me learn about that?
Thanks for response :)
We are interested in locating scientific papers related to mortuary photographs or postmortems, approached from the perspective of the history of childhood.
An example of this type of work can be found in:
BORRAS LLOP, J. Mª. Fotografía/monumento. Historia de la infancia y retratos post-mortem, Hispania, Vol 70, 234 (2010):101-136.
Is it better to use one over the other, or do they produce similar results?
Mostly soft tissues (liver, spleen) and bone marrow need to be homogenized prior to DNA extraction. Fitting into a 1.5ml tube is a must.
Do membranaous inclusions in cytoplasm or destruction of cytoplasmic membranes indicate necrosis or apoptosis?
I have found several articles and reviews talking about this but none of them states it precisely.
What is the best method for forensic autopsy dissection? (Virchow, Letulle or Gohn) and what was the point in reference that the Rokinansky method was confused for the Letulle.
In hospital cases were the anatomical orientation is being learned or vital due to the disease the Letulle method is best for the training pathologist. The Virchow and Gohn are more for forensic cases due to anatomical relationships and time saving dissections.
Rokinansky method is an in-situ examination of viscera with removal of notable organs
Virchow method is an organ by organ removal.
Letulle method is the En Mass removal of all the viscera
Gohn method is En Bloc removal of viscera into Thoracic, intestines, Upper abdominal, Lower abdominal, Brain and neck
We have a group of observers scoring estrogen receptor on a 1-100% scale with known correct answers. The challenge is that the distribution of true answers is binomial and bounded, with many on the low 0-1% end and many on the high 95-100% end. I'd like to know when two observers are significantly different. Thank you.
Performance of an autopsy is “…the single most informative, most instructive, most revealing, most important and mostly costly procedure in the practice of medicine, not merely in the dollars and the cents needed for its proper performance, but most costly from the inescapable fact that every autopsy is carried out at the cost of human life, whether death was the result of natural disease or violence” Dr. Lester Adelson a forensic pathologist once summed up the concept of an autopsy. The autopsy has been used to further medicine and our own understanding. Why has this once great practice faded? What killed the desire to further our understanding of the human body? Are we becoming complacent and feel that we have reached the end of our understanding?