Questions related to Amide Synthases
How can I remove excess amine from these reaction products? I did the reaction which may formed mono and di adduct and also present some excess amine.
I am doing an experiment functionalize CNT via 3 steps: oxidation, acyl-chlorination, and amidation. At step 2, carboxylated CNT was treated with a mixture of SOCl2 and DMF (20:1) at 70oC for 24h. Then the acyl-chlorinated CNT was centrifuged and washed with THF for 5 times before going to step 3. However, I suppose the COCl groups be moisture-sensitive and could easily be reversed to COOH groups when exposing to the atmosphere.
What is the method to treat the acyl-chlorinated CNTs without making the impact on COCl groups?
Most the processes of amino-functionalization of CNTs include 3 steps: carboxylation, acyl-chlorination, and amidation. Why don't we directly do the amidation reaction of carboxylated CNTs with amines? Is it because carboxylated CNTs are less reactive, harsh condition, or low conversion?
I want to hydrolyse the -cyno group selectively into the Amide as well as Acid functionality, I have already tried in Basic madium but no positive result.
I synthesised an amide compound from the reaction between (3-aminophenol and isophthaloyl chloride) in the presence of N,N'-dimethylacetamide as a solvent. After i washed the product with ice-cold deionised water several times to remove the generated HCl, and then recrystallised from hot ethanol and precipitated into ice-cold water again, i got a really pure product, and also a very high yield. However, i need an explanation for the mechanism.
Although I did not use any base to react with the HCl by product, to shift the reaction to product and make it irreversible, the reaction was successful. So what is a reason behind this?
I am now trying to make amide linkage between primary amine and carboxylic groups. My amine is aniline derivative which is less reactive, so I use strong coupling agent TBTU / DIEA. But it gave bad yield. Does anyone have some recommendation about my case?
And I let my reaction happen in normal environment, not Nitrogen or Argon atmosphere. Does it cause serious affect when using TBTU/DIEA?