Algae - Science topic

1. Is algae culture environment requirement different from typical PTC?

2. As per my reading of some of the papers, in case of freshwater algae, it is recommended to use glutaraldehyde, instead of formaldehyde and Lugol's solution. If I preserve my freshwater algae sample with glutaraldehyde, can this sample be used for algal culture afterwards, suppose within a span of 1-2 months?

1. Which institutes/labs in India (Gujarat and others) have facilities for culturing algae, especially freshwater algae or sending the samples for freshwater algae culture?

2. Is the requirement for algae culture separate from typical PTC lab? Do they need completely different lab from PTC lab to give them a particular environment?

3. Which books/research papers should I refer to understand Algae Culture?

As I delve deeper into marine biology, I am particularly interested in expanding my knowledge of the taxonomy, ecology, and physiology of algae, with a focus on macroalgae (seaweeds). Unfortunately, my university does not offer a dedicated course on these topics (Phycology). Therefore, I am seeking to educate myself independently.

Could you kindly recommend essential books, guides, scientific papers, or any other academic resources that would help me gain a thorough understanding of marine algae? Your suggestions would be greatly appreciated.

Thank you in advance for your assistance.

I am a PhD scholar at the Department of Botany, Gujarat University. My work revolves around collecting freshwater algae, and I will also collect water samples from selected freshwater habitats. Which institute, lab, or department in Gujarat can provide portable meters for measuring the physicochemical properties of water on-site? Or if anyone could give the names of some of the portable meters that are affordable to buy, it would also be great.

P.S. It would be helpful to me if there are researchers of Gujarat University or any institute of Gujarat which can provide this information.

The Zarrouk culture-medium was used，and cultured in an outdoor openpond for about half a month, and the picture was taken under 400 times the lens.

A few days ago, I saw a paper discussing the low temperature "vernalization" of microalgae (cyanobacteria). But in the paper, only low temperatures induced the growth effect of cyanobacteria was disscussed. That's an interesting topic. By definition, vernalization is the phenomenon by which certain higher plants must undergo a period of sustained hypothermia before they transition from vegetative to reproductive growth. But species of cyanobacteria have no clear reproductive growth. It made me wonder. Do algae, including macroalgae, have true vernalization like higher plants? If so, how does it work?

We're trying to find a way to test for Bacillus sp. uptake in cyanobacteria but can not determine a way to test if they were taken besides cause-and-effect tracking on fish species that eat the algae.

Perhaps gas chromatography, agar plate growth, etc?

Algae, Algal bloom, phycology

Good afternoon,

Can you please recommend protocols and/or kits for measuring lipids, starch, and proteins in algae (Chlamydomonas and cyanobacteria)?

I would be very grateful.

Hi. I am looking for articles that solely use chitosan as cell wall material without combining other wall materials to encapsulate my algae biomass. I saw mostly published articles use chitosan with different materials. Can you suggest a few papers I can refer to?

It looks like it has no flagela and was showing no movement and has a darker spot in the middle.

I grow spirulina in the lab with a 15L tank. Use aeration and light 24/24. Temperature from 30-36 degrees C. PH from 9.1-9.6

Recently I see that my algae fibers often clump together like moss, under the microscope the algae fibers are broken short. I don't understand why?

please help

I am currently working on culturing Chlorella vulgaris. If it was a contamination, the stock algae should have also been contaminated. But it wasn't, instead it has shown significant growth. I prepared BBM for Standard operating procedure. What would be the reasons?

i am comparing the growth performances of filamentous and non filamentous forms. in a case where I can not count the cells of the filamentous algae using a hemocytometer, is it okay to do a spectrophotometric reading for all algal species involved? and when the species cluster, how do i stabilize the figures on the spec?

Please I need suggestions. Thank you.

Hello, community,

Could you please clarify whether current legislation permits the reuse of algae biomass after it has been used to treat non-hazardous decontaminated laboratory organic waste?

Specifically, I want to understand any regulatory constraints or guidelines that might apply to this process, even if they are not directly concerned with using algae but other biological means (bacteria, yeast).

Additionally, are there particular conditions under which this reuse would be allowed or prohibited?

Cheers,

Gabriele

I collected these from a late Miocene lake deposit. The pictures are taken from thin sections with a monocular microscope. Any help super appreciated!

Thanks, John

Hi everyone,

I'm having trouble with my hydroponic cultures for Arabidopsis thaliana, since lately I've started having algae contamination on them.

I have tried using several sterilization procedures (ethanol+ chloride for seed sterilization; Autoclave for media and other equipment), however they still grow.

Can anyone give some advice on how to manage this problem??

Thanks!!!

Hey there. I'm a Science, Business and Innovation student and for my thesis project I'm currently doing research on different production methods for large-scale cultivation of spirulina. Specifically, I'm comparing raceway ponds with tubular photobioreactors. The comparison I'm drawing is mostly techno-economic, but I'm also interested in comparisons in terms of product quality, sustainibility and reliability. As of right now, most of my research is based on literature and other scientific articles. I would love to validate some of my findings and hear what others think about large-scale spirulina production through interviews. So please, if you are willing to do an interview with me or know someone that might, let me know as it would help me greatly. Thank you in advance

Next step is doing qPCR.

Thanks

Kelps need restoration, especially in such impacted by human being seas like the Black one.

Apart from IMPPAT, TIPDB, MeFSAT and Seaweed metabolite databases, could you please suggest me some more databases for virtual screening study. The target receptor is from Cancer/Diabetes/Spinocerebellar ataxia.

Thank you so much.

Dear Researchers

We are pleased to inform you that we have successfully contracted with Springer Nature to edit a book titled “Industrial and Biotechnological Applications of Algae”. This book aims to bridge the gap between scientific knowledge and practical applications by exploring the cutting-edge research, innovations, and emerging trends in the field of algal biotechnology.

If you have expertise in the field of Phycology, we cordially invite you to contribute a Chapter in this book.

If you and your research group are interested in contributing, then contact @ yadbotany@gmail.com before 31st March, 2024

Editors: Yadvinder Singh, J.I.S. Khattar, D.P. Singh and Rupinder Pal Singh

This image was taken for my research project. It will be a great help if someone helps me with its identification

Thank you!

Scenedesmus obliquus cells are positive or negatively charged?

Hello !

For my microbiology project i need to visualize living P.lunula under a lightmicroscope.

I saw that you can try using Toluidine blue stain, but have not found much research about it.

Algae aquatic ecotoxicity especially on diatoms.

I want to describe the abundance of several algae genera in the intertidal zone, on a mostly rock beach. I cannot use any destructive method (weighing or similar). Checking the percent cover in quadrats along the shore seems like the best option, but there are many details I'm not sure of.

I would appreciate answers for any of the following, and also let me know if there's a better method I'm missing...

1. How do I deal with unevenness of the rocks? My plot would be 2-dimensional, so doesn't the 3-dimensionality of the substrate distort my results?

2. What's better - to subdivide the plot into small quadrats and do the counting on site, or take a picture from above and analyze the plots back in the lab (with ImageJ or similar)? If I do take an image, how high should it be above ground so the image edges are not distorted?

3. How do I maintain the same height (relative to the tide) for all the plots? And not only for all plots in one day of sampling - I need to be able to return to the same site next year and conduct another survey comparable to the first one.

4. Is a 30x30cm plot ok? I've seen people using 50x10 or otherwise elongated plots to have no height differences within-plot. But is that crucial if my "high" and "low" are several meters apart?

Thanks.

Dear scientific community, does anyone know of a #taxonomy course for #microalgae and #cyanobacteria? I'm eager to continue learning and delving deeper into this fascinating field. Any recommendations would be greatly appreciated. Thank you!

1- What are the key factors influencing the efficiency of biohydrogen production by algae?

2-How does the metabolic pathway of algae contribute to biohydrogen production and what are the potential limitations?

We collected paddy field surface sediment that was submerged in irrigation water. We spread on BG-11 agar media, and after that, we incubated at 25 ℃. I think these are phytoplankton due to the PPL express green color. However, this is the first I've seen this PPL as like. Did you ever see phytoplankton like these?

Actually, I am trying to culture seaweeds inside lab for experimental purpose. I'm facing contamination in spore culture and also I can't get a proper growth response with juvenile algae. I m using commercial white fluorescent light, cotton filtered autoclave seawater, pH 7.8 with PES media in 5litre closed containers which having aeration from upside. I have a doubt that I need to do a cultures with opened containers or closed completely. Anyone pls tell me

Hello everyone

I need your help with a problem I can't seem to solven :

I'm planning to do some sequencing of freshwater algae. So I referred to the primer pair made by Stoeck et al. 2010 and Balzano et al. 2015, which is supposed to be general, according to several articles I've read, and quite effective:

Forward primer: V4F (5'-CCA GCA SCY GCG GTA ATT CC-3')

Reverse primer: V4RB (5'-ACT TTC GTT CTT GAT YRR-3')

However, after testing several different PCR cycles and checking on an agarose gel, I very rarely obtain a single band of ~400bp (the desired size).

Most of the time, I end up with either no migration band or several other non-specific bands, including one that is 300bp larger than the desired band.

I have used the cycles recommended by several articles using these primers (Salmaso et al 2020, Latz et al 2022, Balzano et al 2015...), but I don't get any satisfactory results.

I also carried out several tests with different hybridisation temperatures, reduced the proportion of DNA in the PCR mix, added DMSO and reduced the number of cycles, but these did not give satisfactory results.

But unlike most of the articles that use KAPA HiFi HotStart, the basic polymerase in the Swedish studies, I use pHusion HF HotStart Polymerase.

Thank you very much for any help you can give me.

Good luck with your research !

Thomas Charpentier

Throughout the literature, it is unclear whether algal turfs (i.e. dense assemblages of short, turf-forming algae) are a form of algae that occurs due to the effects of disturbance (waves, herbivory) or if they represent a morphological advantage that has evolved over time.

What is this Permian fauna? Is it Dasycladale algae? This speciemen is seen together with plenty of fusulinids.

I'm zoologist, but I would acknowledge very much if someone could supply me examples of known (references) complex species in plants, algae or fungi. Thanks a lot.

Juan Lucas Cervera.

Hi! I found these organisms on a woman's stockings, by rinsing the fragments with serum, and I suspect their presence is the result of friction with a wall covered with mold and/or algae. These are relevant in a forensic case. I think the first 2 represent a fungus, and the last 2 pictures are some unicellular algae. Does someone have a more specific idea of what kind of organisms are in these photos?

I need to know how the colored proteins react with BCA to give the appropriate color which is to be read by the spectrophotometer? I mean I have worked with colorless proteins up till now. How to go about with these colored protein estimation?

in order to be able to make a characterization NMR?

NA

Can anyone help me and recommend a specialist algae doctor to conduct research??

for animal nutrition/ruminant.goat kids

Hai everyone....

I have a medium with added Na2CO3 for algal growth...after somedays algal cells will utilize the Carbonate for its growth...i need to find out how much carbonate ion is consumed by algae after 15 days of growth...Any one kindly tell me the titration procedure for this...

Algae interact with bacteria in multiple ways, ranging from endosymbiosis, ectobiotic symbiosis (in phycosphere, attached on seaweed surface as epiphytes), and non-physically associated but functionally interactive via metabolites. The underlying genomic mechanisms, i.e. how the two partners express their genomes to establish and maintain the relationships, do not seem to be well studied. Any ideas about which genomes have been analyzed in the context of symbiosis with microbes and what the major findings are would be appreciated.

Dear colleagues

We are having a lot of difficulties removing green algae from a duckweed taken from a pond. We tried several attempts with sodium hypochlorite at different concentrations until the wild duckweed borders turned white. Also, we tried covering the growth flask with aluminum foil, and this prevented the algae growth, but after removing this cover, we noticed that the algae were still there, and it started increasing again...

I would appreciate it if you have any suggestions or recommendations.

Thanks in advance

What specific natural plant nutrient sources or plant growth-promoting sources, such as BIOSTIMULANTS, BIOFERTILIZERS, etc., would you use for starting cultivating tropical crops like corn, sorghum, millet, peanuts, tomatoes, and onions in a middle scale production in a tropical country as Simbabwe, where chemical fertilizers are economically not afordable or either unavailable, but where some animal dung is accessible?

How economically successful is it which commercially available mycorrhiza to use or other microorganisms of the soil microbiome with similar benefits such as PGPR (plant growth-promoting rhizobacteria), PGPF (plant growth promoting fungi), PGPM (plant-growth-promoting microorganisms), as well to use seaweed, algae stimulants or verimcompost?

For biofuel production from microalgae

The size differ little bit from one species to another, yet they have one size range. Also, the size of them in their native form so they don't lose their colour while isolation.

the best culture medium or media?

some specific steps to be considered and precautions and then culturing at a higher scale.

Green algae or blue green algae?

I am planning to cultivate algae-bacteria biofilms under greenhouse conditions using biofilm carriers. I have routinely come across Industrial Soft Carriers as frequently used substrates for biofilm development. What are these carriers commonly made of? Are they based on plastic polymers?

Additionally, suggest any specific type of biofilm carriers that are particularly helpful in cultivating biofilms for later use in plastic bioremediation processes.

Much thanks.

I would like to perform a multiple displacement amplification experiment on microalgal cells（possibly diatoms, dinoflagellates, cryptophytes, gold algae, ciliates, etc.）.Due to transportation issues with the samples, I fixed the microalgal cells with glutaraldehyde. Could you please advise on an effective method for cell lysis of these fixed algae cells to adapt to the subsequent MDA reaction?

In algae i care about cyanobacteria

salam alaikum

is this Raphidonema?

Previous I used colchicine to induce polyploidy in eukaryotic algae but now I want to know is the same for prokaryotic algae such as spirulina or not?

Hello. I am a post-graduate student and I am doing a research on the blooming of bioluminescent algae. I came across this organism in my sample and failed to identify it. If anyone of you know what is it, please let me know.

I will ferment ulva substrate with saccharomyces cerevisiae yeast. Can I add it directly or do I have to do the activation process first? If there is a need for the activation process, can you help me with the procedure?

Is algae fertilization considered biological or organic if the algae is dried?

Dear all,

I will really appreciate if someone could give me some suggestions to how to embed (fixation, postfixation, which resin) and in particular how to cut Diatoms algae, in order to investigate the plastids ultrastructure by transmission electron microscopy. Because their skeleton is made of silica, the diamond knive will be destroyed for sure, how to overcome this?

Many thanks!!!

Francesco

i'm looking for a good, general eukaryotic (?18s) primer that would amplify in most protists and eukaryotic single celled algae in pond samples. Can you suggest a good one?

Some freshwater species difficult to identify. Please help me find this species.

I want to do liminology of my fish pond. My major concern is with different types of algae present in the pond. ....kindly share your experience regarding sampling of algae and cell count per liter....

Samples from freshwater, surface water.

Some of the freshwater algae and diatoms we find. But this species identification not easy. Anyone expert help me to find the genus or species?

I am waiting for your support.

Thanking you!

By,

Vijayan

I am analyzing trace metal concentrations in algae pellets, which have been digested in HNO3. Some of the media used to grow the algae have very high concentrations of macro-level nutritional elements i.e. Na, Mg, P, S, and Ca. The range of concentrations is from 1000 ppm to 10,000 ppm. Can I run these samples directly on the ICP-MS without damaging the mass specs/multiplier?

I expect issues like signal suppression and deposits on the cone, but the main concern is not damaging the ICP-MS.

In a study in which we want to study the rhodophyte Gracilaria vermiculophylla with PAM fluorometry (diving PAM) we are facing the problem that this small tubular algae doesn't cover the complete area of the dark leaf clip. How can we correct the measurements?

Currently, I use pure water as my eluate. I wanted to use NaN3, but our company won't allow me to use that due to safety issues, so I wonder if there is something else I can use to prevent algae growth?