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Algae - Science topic

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Classification and forms prepared in each season of the year
📷
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Kindly check the following link that examine the relation between submerged macrophytes, their epiphytic microalgae and bacterial communities as well as the variations in their distribution and species composition with respect to season and location.
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How and why is growth of benthic algae a disadvantage to the closed microalgal photobioreactor?? Basically also how benthic algae grows in closed pbr having a different algal strain
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Many benthic algae grow attached to the substrate, i.e. walls and other structures of PBRs, which makes them impossible to harvest, they may also release from the substrate in patches and clog the technology (pipes, pumps, filters).
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Recently, as a result of an increase in water temperature and a decrease in the concentration of dissolved oxygen in the water, whitening of corals has been recorded (death of algae in the symbiosis of algae and polyps). Why have colonies of algae colonized coral reefs in the Red Sea in recent years? What is the reason for this contradiction?
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I think the most important thing to consider is the type or species of algae colonising those coral reefs and the optimal growth conditions. Some (especially tropical) algae are highly invasive and can withstand highly hostile conditions. Moreover, corals are sensitive to the slightest changes in their environment.
Perhaps, the thriving species are invasive one that have been introduced through the common pathways, such as ships, accidental discharge and fishing gear.
Or maybe, the situation is similar to terrestrial weeds vs flowers or vegetables in a garden or farm. There is not only competition for nutrients; the death of the corals could be contributing to the aggressive growth of existing algae in the Red Sea.
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Currently, I use pure water as my eluate. I wanted to use NaN3, but our company won't allow me to use that due to safety issues, so I wonder if there is something else I can use to prevent algae growth?
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How long do you need to keep the mobile phase? If the chemicals are not expensive, make new solutions of the MP in clean bottles every 2 weeks. You may be able to use sodium benzoate. If you are concerned about algae, keep the MP in the dark.
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  • It is found in rock crevices
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Picture quality is poor. It may be some species of lichen.
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I am working in the green flagellated algae, I want to do live cell imaging for studying colocalization experiment. Please help me out with that.
Actually I am facing problem that cells are mobile but for imaging they should be fixed. I need to do colocalization experiment for two proteins in cell. So, kindly suggest me any suitable protocol or lab who can help me with that.
Thanks
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Thanks @ Carlos Maximiliano Szkope-Cobo for your help but I need to do live cell not after fixation of cells. I am already working in protein localization in algae using immunolocation after fixing algal cells.
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Hi everyone,
I hope all is well . I was wondering if anyone can recommend a solution that's safe for primary cells and iPSCs to add to the incubator water bath to aid in preventing microorganisms from growing. I have been using the one from RPI(algae inhibitor) but I am not 100% sure its safe for use in incubators. Anyone have other recommendations other than Aquaguard (can't find it anywhere to get in California)
Thank you !
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Just use dustilled water it's better than any reagent
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For the built environment, AgNPs have been mostly explored for their efficacy against fungal biodeteriogenic strains while for such kinds of algal strains, TiO2 NPs have been explored extensively. If both algae and fungi are eukaryotic, why is there a particular preference for an antibiofouling agent for the two different kinds of organisms?
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Kindly check the following link that may be useful:
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I want to learn as a species.
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I agree that Synedra is a likely ID but you may also want to consider Pinnularia. They are both pennate diatoms with a raphe and similar morphology. There are some slight differences in the shape particularly in the terminal morphology. When you encounter this taxa again carefully read the descriptions to tease out which genus you in fact have. A very good guide book to use is
Identifying Marine Phytoplankton Editor: Carmelo Tomas https://doi.org/10.1016/B978-0-12-693018-4.X5000-9
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Previous I used colchicine to induce polyploidy in eukaryotic algae but now I want to know is the same for prokaryotic algae such as spirulina or not?
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Dear Ms. Hakimeh,
I share a conference paper related to colchicine application on Spirulina platensis culture. The colchicine effect is evaluated by trichome diameter and Phycocyanin content, but a more precise aproach is needed to quantify the ploidy level, for example by Flow Cytometry.
Hope you find usefull.
Best regards.
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This algae invade my culture lately (Chlamydomonas). It will caused the culture to clump and eventually die. This contaminant was motile, with more than 4 flagella observed.
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Dear Jess,
probably it is Poterioochromonas, a golden algae. You can find some information here:
Best wishes
Eleftherios
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I had problems raising fresh water algae in Bold Basal Media. The chemical components in the media tend to react so much, even though they are mixed thoroughly after adding one by one. Moreover fresh water algae Chlorella vulgaris could not grow well in it. Even after making dilute and transparent media, the algae could not grow. I even doubt the quality of the algae I received.
I have raised marine algae and diatom in my previous research and even used them to treat textile effluent.
Please advice if I can use marine algae to treat domestic waste water (even though its source is fresh water) or should I use the sensitive fresh water algae? Please advice?
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Sri Hari depends on the salinity levels of the water if itwsw above 2.5 levels saline then go for marine or fresh algae is always a option
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Peace, mercy, and blessing of good. I am trying to blast algal nucleotide sequence (purified using primer target 18S rRNA) and the result did not show any alignments as well the search for the conserved domain didn't show any result. Any suggestion?
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Yes, the NCBI database includes algae. Make sure that you aren't accidentally choosing human or some other subset as a default.
What do your .ab1 files look like?
Good luck!
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During a group project, we work on a problem encountered by a farm is aquaponics. We need to develop a solution to reduce the nitrate level of the water leaving the system (100mg/L to 50mg/L), while producing value-added biomass (plants, algae?) that can be sold.
So, we are looking for a plant or algae that strongly assimilates nitrate and that can bring a benefit to the aquaponics farm. Do you have ideas for innovative solutions? techniques to optimize space and growth?
For now, we were thinking of using microalgae, Nelumbo nucifera, cress or pak choi. Are they interesting for our mission ?
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Thank you @ all of you.
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I'm working on determining the composition of algae after culturing it with wastewater.
I plan on using a lab-scale centrifuge for algae harvesting. What speed and time are suitable for a 40 ml algae culture?
Any protocol or procedure available will be helpful.
Thank you.
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1800 rpm / 4 min, i worked this condition in my project. Centrifuge model was Digtor 20-c / Orto Alresa, I wish you successes
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Hello all,
I have been trying to revive the algal (chlorella minutisimma) culture stored in the fridge having fungal contamination for the past 4 months. During all my attempts after 10 days, my culture is getting faded and losing its green color. I have added penicillin (622.5mg/L) streptomycin (250 mg/L) antimicrobials and bavistin (6mg/L) and fluconazole (100mg/L) antifungal agents, but still, fungal growth is there and no algae are growing. Kindly suggest some technique or enrichment strategy to boost the culture.
Thanks in advance
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If possible, the best is to buy a new standard culture of the algae. If buying a new culture is impossible, you can try single-cell isolation using sterile glass pipettes and a microscope in the sterile hoop, it will take time and effort.
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I recently cultured a Microcystis aeruginosa strain, however it was contaminated by bacteria. I have tried to use solid medium to purify it. I used 0.4% agarose as the gel, and sterilized the agarose and BG11 medium separately and combined them together after the temperature decreased to ~40 degrees centigrade. Culture of Microcystis aeruginosa was spread over the midium. Two weeks later, only some bacteria grew on the plate, while no algae grew. What went wrong? How to improve my solid medium?
Or are there any other methods to purify a bacteria-contaminated algae except using the solid medium?
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We have used an antibiotic method to produce axenic algae cultures. This might work with your one. If you talk to a microbiologist, they have a kit used to test sensitivity of bacteria to many antibiotics. Your culture is spread on agar and different paper disks containing different antibiotics are placed on the agar plate. Look for a clear zone around a paper disk where the bacteria are killed but your alga is not affected. This tells you which antibiotic to add to your culture medium. Our technique was published previously as “One step antibiotic disk method for obtaining axenic cultures of multicellular marine algae.” Bradley et al. 1988. Plant cell, tissue and organ culture 12: 55-60. Available on Peter Bradley’s ResearchGate place. Good luck with your work.
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I am dealing with the presence of Lemna Major in WWTP secondary settling, is it a positive or negative presence? I guess they are helping the reputation process but it can lead to full covering of the settling
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I would consult., "Standard Methods for the Examination of water and Waste water" available on line as well as from "American Water Works Association".
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There is a recent increase in temperature for which there might be a disbalance of fish and algae population in the pond ecosystem. I was wondering if anyone working on this problem based on population dynamics of fish and algae - interactive mathematical model.
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I'd like your advice on Pyrocystis Fusiformis growth. I purchased a culture a month ago and began cultivating it right away. Things, on the other hand, do not appear to be moving forward. There has been no bioluminescence observed yet, and the algae's growth has been poor. The culture was maintained at a pH of 8.2 and a temperature of 20 degrees Celsius with a 12-12 dark/light cycle. Cell counting under a microscope was used to monitor the algae's progress, but because I don't have much experience in the field of microbiology I wasn't sure of my count. I've attached a photo of what I see under the microscope, and I'm not sure if it's algae cells or not.
I'm hoping you could give me some suggestions on how to improve the growth of algae and its bioluminescence.
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Barry C Smith F/2 media was utilized, along with a quite bright LED light (5000 lux measured using a lux meter app on the inoculum surface). The culture volume is 4L, while the inoculum volume is around 6L.
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I have tried to extract nanocellulose fibres from algae powder and have yielded 15% of the total biomass taken. I want a suggestion for how to extract maximum yield of nanocellulose from it.
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This other research could be useful for you:
Best,
Fran
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Extracted fucoidan and alginate from algae and carried HPLC and FTIR. FTIR spectrum was matching with that of standard. But, for HPLC I didn't get any peaks for sample ( got peak for standard). Hydrolysis was carried in different ways ( TFA and H2So4) to get peaks but still no result. Column specification: Hypersil Gold Amino, 250 X 4.6 mm, Particle size 5 micro. Can any one help me get peak for monosaccharaides in my sample?
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I am currently working on culturing Chlorella vulgaris. If it was a contamination, the stock algae should have also been contaminated. But it wasn't, instead it has shown significant growth. I prepared BBM for Standard operating procedure. What would be the reasons?
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You may check the culture conductivity, too.
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I am currently working on cultivation of Chlorella vulgaris. I am just curious to know what happens. Thank you in advance.
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There will be pressure development inside the reactor; eventually, algae may die, sparging would be stopped, or the container would explode due to high pressure in the container.
Thanks
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I am currently working on culturing chlorella vulgaris. I have EDTA disodium salt instead of EDTA tetrasodium salt to prepare bold basal media. Does it affect the species?
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Dear Jayathraa Raman thank you for posting this very interesting technical question on RG. Unfortunately I'm not really an expert in this field of research as we work mainly in synthetic inorganic chemistry. However, I noticed that there are a few closely related technical questions which have been asked earlier on RG. It might be helpful reading the answers given to those questions. Thus please have a look at the following links:
Can I use EDTA (MW: 292) instead of Disodium EDTA (MV: 372)?
(6 ansers)
and
What is different of EDTA, K2 EDTA, and EDTA (disodium salt)?
(5 answers)
Also please check the following relevant article:
Difference Between Disodium EDTA and Tetrasodium EDTA
I hope this helps. Good luck with your work and best wishes, Frank Edelmann
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I am working with algae and i have to formulate diet for fish (commercial+algae) i calculated cell count which was 8.76x10^6 cells/mL, now i want to add dried form of this algae in fish diet keeping concentration 1x10^5 cells. How do i convert cells/mL into cells/mg
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Yes Hamza Khan is correct, or cells/mg = cells/ml x ml/mg :)
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My name is Stanley please help me with the name of the microscope that I can use to view the microalgae structure and able to isolate a single strain.
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I would recommend an inverted microscope with sedimentation chambers, so you won’t have to squeeze your algae between slide and cover glass. This also means you can use magnifications of 630 times, maybe even 1000 with immersion oil. Use both live samples and lugol preserved ones if your specimens are too mobile.
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Hi, when I DNA extract from sargassum (brown algae), I did not see any band on agarose gel or band is very weak. please help me.
thanks
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Maurissa Sakti .. Could you share the protocol you used to get the best result or at least the good one among all protocols you tried? Maybe someone here could give a suggestion to solve the problem by modifying the solution or some step to make it a better result.
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I have already identified chitosan, guar gum, and moringa oleifera seed powder as potential flocculation agents. However, the available information is sparse. Any help and advice is highly appreciated! :) Merry Christmas to everyone!
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Firstly, Spirulina (Arthrospira platensis) is a filamentous and multicellular blue-green alga. Eventually, it doesn't require too much effort for harvesting.
Secondly, using chemical flocculants for harvesting microalgae is not advisable for food/pharmaceutical applications because the chemicals might have toxic effects on the final products. On the other hand, physical harvesting, for instance, moving net, could be promising.
Please check the video link below:
Thank you
Best of luck
Chandan
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My Students are working on photo bioreactor to grow algae. Bu there are few challenges we are facing currently. Such leakage, how should CO2 be introduced to reactor and what amount? Other challenges solution can also be helpful.
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Dear Dr. Aftab Ahmed
First of all, we need to know the exact problems you are facing with the photobioreactor. For leakage, Teflon tape can be used as a thread sealant, or you can use chloroform to seal the leak permanently if the reactor is made of acrylic material. CO2 should be sparged from the bottom of the reactor. The amount of CO2, you will get from literature.
Thank you
Chandan
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Hello.
I have water and sediment samples collected in freshwater, brackish water and marine water environments. I want to keep these samples for a long time and use them in the future for taxonomic analysis of diatoms. These are not cleared samples. So If I replace most of the water by alcohol, how long can I keep these samples?
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algae index , water quality
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Jasim m. Salman experts have already nailed it particularly the formula shared by Uthirasamy S. and the links of Avinash Kumar and Omar Farouk Al Mashhour are the best ones and I liked the links and the data shared about the ALGAL index estimation and the assessment of ecological status using the phytoplankton inices,,,Thanks
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Can any one suggested an author processing fees free research journal for publishing the algae related work...The journal should not be a predatory one.
Example: comparative study on algal diversity in three different study sites.
Thank you
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Journal of Coastal Research
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Bioinsectisides are popular now for crop protection. The proponents would like to ask some recommendation for capturing VOC compounds from marine algae the is easy. What formulation of non-toxic aerosol type bioinsectiside can be compatible for VOC.
Hypothetical Material:
Active Ingridient - VOC from Marine Algae
Inert Ingridient - ???
Thank you.
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These plants also include Cinerarieliste Feverfew (Dalmatian chamomile), the extract of which served as the basis for a bio-insecticide. Such a seemingly harmless flower grows on the Balkan Peninsula off the coast of the Adriatic Sea, and is also cultivated in the North Caucasus, Central Asia and some European countries.
As a result of high-tech processing, Pyrethrum (pyrethrum) is obtained from the inflorescences of mountain chamomile - a contact poison that enters the insect's body through the chitinous cover or the respiratory system, and then spreads through the body through the cavity fluids, causing paralysis of the nervous system.
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Need to know what are the possibility of fixation of sodium (as algae is developed in samples).
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Dear Naresh, thank for posting this interesting technical question on RG. As a synthetic inorganic chemist I'm not a specialist in this field of research, but I think that the following relevant literature reference could help you in your analysis:
Determination of concentration of total sodium and potassium in surface and ground water using a flame photometer
This useful article is freely available on the internet as public full text (please see attached pdf file). Good luck with your work and best wishes!
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I know that this depends on the rheological factors of the medium, but if you know any, please let me know.
Best Regards,
Roberto Novais
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You have to calibrate according to your growth conditions.​ Make​ a​ standard​ caurve and​ compared batween OD and​ cell​ counting
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I am extracting oil from algae but all the procedures that I have seen call for a rotatory evaporator as the last step to evaporate the hexane. I don't have a rotatory evaporator so I was looking for other valid and accurate ways to evaporate the solvent. Can I use a water bath?
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You can, but you need to install a system to cool the evaporated solvent and collect it.
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Which culture media is suitable for cultivating symbiotic algae at laboratory condition and why it is highly prescribed. Can we get arsenic culture through the prescribed method??
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@Wei Ding thank you so much for your answer
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Hi Friend and colleague,
Anybody is working in field of algae can respond to me. I had query about Pamer index. 1969 and Shannon index .
Which index is better for studying water pollution in relation to algae
Because some of research article showed use of
Staub et. al. (1970) : shannon diversity is based on diversity index D
3.0 - 4.5 : Slight pollution
2.0 - 3.0 : Light pollution
1.0 - 2.0 : Moderate pollution
0.0 - 1.0 : Heavy pollution
Wilhm and Dorris (1968) proposed the following relationship between diversity index (D) and pollution status. D Condition
> 3 Clean water
1 - 3 Moderately polluted
< 1 Heavily polluted
Trivedi (1980 and 1981) on correlation of diversity index (D) and the degree of pollution as follows : Condition
> 4.0 : Clean water
3.0 - 4.0 : Very light pollution
2.0 - 3.0 : Moderate pollution
< 2.0 : Heavy pollution
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Shannon Weiner indicates the degree of better/stressful environment.
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Hello,
I recently have started in a new position analyzing seagrass and other submerged aquatic vegetation (SAV) within an aquatic preserve. Our data is collected using a modified Braun-Blanquet (BB) score where if a species is present in our quadrat we give it a score of1 (less than 5%), 2 (5-25%), 3 (25-50%), 4 (50-75%), and 5 (75-100%). Historically they had been analyzing this data using average BB scores and percent occurrence. In each system we collect four quadrats from 25 sites and then the system as a whole is analyzed. I have not used BB methods before so I was looking into ways to analyze and learned basically that averaging these scores is not a good method for analyzing BB scores. Does anyone have any suggestions or insight on the best way to analyze this data? I've been reading from many different sources that all suggest different methods. I was wondering if anyone can help point me in the right direction for starting out, we have large datasets with 5 species of seagrasses and multiple species of algae. Any advice or suggested reading is highly appreciated.
Thank you
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I have two questions. I would appreciate it if anyone can help me with these questions:
We have a raceway pond in which O2 is continuously produced due to the presence of algae. We know that the water can dissolve O2 up to its solubility which I think is 40 mg/L (under normal conditions). My question is that what would happen after the water is saturated with O2? What is the fate of O2 that is produced after the saturation point? Do they rise up to the surface and then diffuse into the air? Is there any chance of bubble formation in this case?
And the second question is: If the water is saturated with O2, does it prevent available CO2 in the air to be absorbed into the water? What is the reaction between the O2 and CO2 in this case?
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Kindly look at this experiment done at Harvard
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Respected sir/Madam expert working in field of algae and Diatom, I found following article on,
T. V. Ramachandra Malvikaa Solanki. 2007. "Ecological assessment of Lentic water bodies of Banglore. "
This is the formula for that, I found it, these is easy method for counting density of plankton. Can any body had reference of it.
i am using 25 (L) of water to collected in 50 ml bottler, and tanking 0.1 ml for sample for analysis on normal slide and cover slip.
Total plankton count per litre = A * (1/L) * (n/v) Where, A = number of organisms per drop             L = volume of original sample (l)             n = total volume of concentrated sample (ml)             v = volume of one drop (ml)
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Hi,
I'm trying to identify the alga I marked in the photo. Can you help? Thanks for your help.
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review genus Zygnema
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Can anyone explain the method in details
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Thank you so much, where is the attached paper?!
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Dear community,
He have observed several times in our reactors a contamination by an unknown filamentous algae.
It forms greens patches of biofilm at first, which grows to form green/brownish thick biofilm with time. Under the microscope (see pictures), it appears like thin pale green filaments, rarely in suspension (concentrated in biofilm however).
We cultivate our spirulina in Zarrouk medium, and this contaminant is a huge problem for us.
We think it may be Phormidium sp.
Do you have any hint on its nature, and how to get rid of it ? (or at least control it)
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Anh Nguyen: although the NaHCO3 is a good buffer it can not raise the pH. In fact, when algae use It as a carbon source It can turn into NaHCO3 and raise the pH but in low density, there isn't enough biomass. to increase the start pH the best option is NaCO3. If you don't have access to NACO3 don't be worry! you can heat NaHCO3 in the oven at 200 C for 2h or simply put the NaHCO3 in a pan and heat it until the color turns whiter and the particles pop up.
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We have the hatchings of Batagur Dhongka at the Rescue and Rehabilitation Centre. They are healthy and growing well, but the problem is that algae have been found on their carapace. We used to remove it manually, and the algaes are coming back next month. The water quality/aeration is perfect. We are changing it twice a week. We have other species as well, like Batagur kachuga, and they have never found these algae. Would you please tell me some tips on how to remove algae from the Batagur Dhongka carapace permanently?
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Animesh Talukdar Bernardo Antonio Perez Da Gama Kiprotich Kiptum Thank you for your suggestions. I will look into it.
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I have separate algal and zooplankton samples that I want to have stable isotope analysis of C and N performed on.
The samples were filtered onto 0.4 µm glass fibre filters (GF/F) that were then freeze-dried and stored at - 80 degrees. In error, the glass fibre filters were not pre-combusted or pre-weighed prior to having the zooplankton and algal samples filtered onto them.
Is there a way to still use these samples for stable isotope analysis of C and N? I have read that you can scape the material of the filter paper and then place in a tin foil boat for analysis but don't feel confident in the reliability of this approach.
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Hi !
If you get satisfactory results after checking the blank filters, you can use the scraping method for d15N. Just scrape off the layer of filter paper from behind without disturbing the top layer (avoid any contamination while doing so). For d13C, use a part of filter paper if the content is too high after acid fumigation.
Hope this helps !
Prachi
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Hello everyone, I am planning to do a 13C stable isotope labelling experiment in algae, it is going to be a pulse-chase experiment, where I will be feeding the algae with 13C isotope labelled substrate for 4 days, I will be keeping the cells at a metabolic steady-state by maintaining the cells at a constant optical density (like a chemostat), after maximum isotope labelling, I will be transferring the cells to an isotope free media and deprive the cells of nitrogen to track the fattyacid reshuffling into Triacylglycerol molecules.
What software packages do you people suggest for analysing my LC-MS and GC-MS data?
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Mr. Potlapalli,
In order to, obtain exact quantitative information about your analyte by mass spectrometry you need (i) instrumental setup software; (ii) free of charge AMDIS software [http://www.amdis.net/]; (iii) calculator; and (iv) Microsoft Excel, which is installed routinely on each computer, respectively.
Why?
Because of, accurate, presice, selective, sensitive and exact quantification by mass spectrometry, currently, can be carried out only within the framework of our (to me and my co-author's according to the shown authorship below) most recently developed stochastic dynamic theory and model formula, which show linear correlation coefficient between theory and experiment /r/ = 0.99997 - 1 at concentration levels of analytes pg.(mL)-1.
Please, consider works [1,2], in addition to, reference [3].
1] Stochastic Dynamic Mass Spectrometric Approach to Quantify Reserpine in Solution; Bojidarka Ivanova, Michael Spiteller Analytical Chemistry Letters, 10 (2020) 703-721; Received 13 Oct 2020, Accepted 16 Dec 2020, Published online: 28 Jan 2021 Download citation https://doi.org/10.1080/22297928.2020.1865834
[2] Steroids, 164 (2020) 108750 Stochastic dynamic mass spectrometric quantification of steroids in mixture — Part II; Bojidarka Ivanova, Michael Spiteller.
(The presentation is available on the Conference's web-page [https://ecmc2021.sciforum.net/] (section: General,) as well)
AMDIS software provides absolute intesity value per span of scan time according to the theoretical framework of our model equation. These intensity values are calculated according to the model equation by calculator. The linear correlation between the parameters of our formula and the analyte concentration in solution is carried out by Excel.
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We conducted experiments to measure the sponge bioerosion rate on coralline algae based on the alkalinity anomaly technique. From the experiment, we calculated the change in total alkalinity (ΔT) (µmol/l). Further, the formula for calculating the mass of Calcium carbonate dissolved by erosion is
M (CaCO3) = 0.5 (mol eq-1)x ΔT x 100 x Vsw x ρsw
Here, ΔT = change in alkalinity in eq kg-1
100 = molecular mass of CaCO3
Vsw = Volume of seawater (L)
ρsw = density of seawater
Looking forward to some method/formula to convert the Change in Total alkalinity value in µmol/l to Eq/kg.
Thanks in Advance.
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Hello,
As far as I remember 1 litre of seawater at 20°C and salinity 35 weights 1,025 kg (density) so to get your conversion you have to divide the value per liter by 1,025, but this works only if temperature and salinity are as above, for other values you have to look at hydrographic table to get the exact water density.
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Our team has an imaging PAM fluorometer (Handy fluorcam fc 1000-h (PSI) + Fluorcam 7 software) and we work with rock substrate (especially granite) colonized by phototrophic biofilms. However, we encountered the problem that the substrate generates erroneous readings and incorporates a lot of noise to the final results (either due to bare rock or an inhomogeneous coverage of phototrophs).
This generates histograms of maximum yield, NPQ, qP,...with negative values or with too large values. The software allows to remove these values outside the biological range (at least, the negative values and the over stimated positive values like +40 NPQ, qP,...), however we doubt that the final result will be significant given the elimination of points in the image or by keeping noise from the rock that has values within the biological range.
Any advice on how to get the best results? Either on how to prepare the samples, the settings of the apparatus or on the post-processing with the software. Due to the great complexity of the PAM technique (especially in imaging) we have doubts if the final data are valid or not since the device is designed for leaves, and there is not much information about PAM in rock substrate.
P.e. we tried to set a minimun value of Fm. In that way, we can (more or less) remove the rock part, but we are not sure if we are also removing part of the chlorophyll signal (we are talking about a minimun value of 70-100 of Fm)
The organism growing in rock are very difficult to study because its growth its not optimized (they use the rock as a nutrient source, water is not constant,...).
I wanted to start this discussion to try to create a debate among experts to help us and to interchange ideas to help better this particular research (i.e chlorophyll fluorometry on rock surfaces), because its a new field for our group and me and my collegue are phd students still learning a lot about this!!
Thank you all very much!
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We will try those recomendations! Thank you very much
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Hi Everyone
Could you please name journals which publish review article related to Algae Biotechnology for free within less duration.
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You can try Trends in Biotechnology (IF: 14.343)
Thanks
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I've been growing my strain on Solar System 1000 light on full power. Also I've added a CO2 injection system recently to help with growing. I'm growing it in a Proteose peptone medium and adding an Airstone to help with Oxygen. Is there any advice one of you could give me? Cause it doesn't seem to be working.
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Initially make sure a very pure nutrient medium (Autoclaved water, sterilized flasks, culture area, air balls, and optimum light intensity). Make a balanced nutrient medium (F/2 or Zurroks) considering major and trace elements including vitamins. Initially, interval aeration is recommended. Once you can easily grow your culture when your stock will be healthy.
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The aim is to extract cyclic peptides, alkaloids, diaminoacids,
And at the same time get rid of the pigments and phenolic compounds
We tried with 80% MeOH in water, but still there are lots of pigments hiding our analytes.
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First, you should investigate what polarity the metabolites of your interest have and according to that, you will look for solvents that have a similar polarity. You can also base on the extract you already have and perform liquid-liquid extractions with immiscible solvents to eliminate the compounds you are not interested in.
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sample is taken from freshwater pond .600×
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Eudorina is my suggestion. It could be correctly identified if photomicrographs with better resolution are provided.
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hi
I am looking for information about separate peridinium algae from water .
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thank you for your answer. unfortunately, in my opinion because of being as a micro algae, peridinium do not settle by gravity .
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I'm working on a research project to study the effect of light shading on algae control. I was wondering about what happens to the TOC values if the dead algae dissolves into the water, and if there is any research papers that explains this?
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Hi Mohammad
TOC, as it implies, is the total ORGANIC C which is in the structure of organic molecules (hydrocarbons). When the algae dies, it starts to be physically and chemically decomposed by the existing bacteria and other microorganisms in the water. Some of it also starts to decompose by the chemistry of the water (especially in acidic waters, pH<6.6). Therefore, the organic carbon gradually will be converted to CO2.
This process will result in a significant increase on TOC at the first phases when the physical decomposition is going on. But after chemical decomposition by bacteria and water chemistry starts, the water TOC starts to decrease. The rate of chemical decomposition of TOC by bacteria and chemical interaction in the water can be determined by measuring BOD (biological oxygen demand) and COD (chemical oxygen demand) in the water.
Hope it helps
Hanieh
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I found two different algae, both coming from freswater shallow ponds (not the same), and I do not know what species they belong to.
1. Regarding the first photo, I thought it was a fragment of Volvox, but I can't see any flagela, and there are not any connection among cells either. I thought of something similar to Tetraspora after, but in that case I would have expected the cells to be in tetrads.
2. Regarding the second photo, I think it may be Cladomonas but I am not sure at all.
I would be very grateful if you could help me to identify any of them.
Kind regards.
Gonzalo Martín
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Helpful
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In order to study the optimization of moroccan algae biomass, we're monotoring the growth of 4 different green algae in a laboratory setting in liquid medium under different LED light intensities.
As the culture progressed, we noticed severe discoloration and loss of integrity of one of the macroalgae cultures. However, the rest of the algae species were not affected and remained green.
  • What could be the cause/causes of these changes? Is it a fungal contamination or a sign that this algae reacted negatively to the light intensities chosen?
  • What would be the best approach to investigate the cause and to determine it?
Any suggestion would be highly appreciated.
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Decoloration est dur aux rzyons solaire ou n'importe quels paramètres qui peuvent dégrader les couleurs
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I am trying to program a model which indicates the health of mangrove forest in west coast of India and trying to find elements which might be working as indicators of health. Can anyone help me with such indicators and their relation with mangroves?
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This paper can give you a lead
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In a recent paper submitted for peer-review I made the statement that ... "Despite their ecological importance and their ubiquity they (the coralline algae) are still a comparatively poorly known group of marine organisms whose taxonomy has remained in flux".  A reviewer commented that this was not correct because “if you look at the number of papers on corallines covered in scientific abstracting databases, it is actually correct to say that in the last 10-15 years corallines have been one of the best-studied algal groups".  I am not disputing the increased number of papers or the numerous scientists that have extensively worked on this group in recent years, but what I am suggesting is that despite all our efforts, this group still remains a largely poorly understood group of algae. This is evidenced by the extensive work on this group in recent years in which much debate on their taxonomy and phylogeny still remains.  I do agree though that our understanding of their taxonomy is expected to improve as we better understand their molecular characterisations.  I would be happy for any comments on these statements.
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Looking at its wide global distribution in the marine systems, yes, the coralline algae are still poorly known and deserve lot more recognition. Complex taxonomy is an issue but I think its poor applicability in biostratigraphy (obvious due to their very long, persistent stratigraphic records) makes them less attractive to researchers in comparison to several other marine groups.
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I am currently advising a young student in the National University of El Salvador and he has an interest in working with remote sensing data to study freshwater quality (turbity, pollution, algae blooms, etc). The available data to use is the Copernicus/Sentinels open data from the European Space Agency. The university periodically conducts in-situ studies of the water and collects samples along with the National Ministry of the Environment and Natural Resources.
We think that exploring the correlations between temperature, suspended matter, clorophyll (algae) and data from Sentinel 2/3 (some initial exploration attached). But this is just a very initial/raw idea.
Therefore I would like to be advised on relevant topics in this field of research that are of interest to the global community and not just El Salvador. My ambition is to conduct a research task that can be contributed to international peer-reviewed journals and establish relationships with experts and research groups abroad.
Any suggestions, ideas, contacts will be greatly appreciated.
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Hola Napoleón, actualmente la UCA desarrolla una investigación interdisciplinaria con biólogos, expertos SIG en teledetección y expertos en ciencias de la computación, en un proyecto de monitoreo del crecimiento de algas y toxinas en el embalse del Cerrón Grande, puedes contactar con Metzi Aguilar que coordina la parte de teledetección maguilar@uca.edu.sv y Luis Cierra biólogo jsierra@uca.edu.sv , creo que pueden tener buena sinergia!
Saludos
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I'm looking to describe an algal community, so I need a method that can work to preserve both diatoms and other algae. I've been looking at glutaraldehyde, but I'm not sure if that is the best option. Any help is appreciated!
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Hi Willow;
Phytoplankton are fixed with a Lugol-glycerol solution (2-3 % glycerol). Glycerol is required for flagellates. Please look at European Committee for Standardization, 2015. According to the standard method (European Committee for Standardization 2006), phytoplankton enumerations were carried out under an inverted microscope (Olympus CKX41) at magnifications of 400 and 600X. For each sample, at least 350 settling units of the dominant species were counted. Phytoplankton biovolume was calculated by multiplying the cell density and mean biovolume of the taxon, which was taken approximating geometric shapes of the least 25 individuals (Sun and Liu 2003).
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I was wondering if algae with a lot of oil produced float to the top of their suspension culture based bioreactors - wouldn't this mess up the bioreactor system? (e.g algae all bunched to the top so there's less mass transport of nutrients and waste etc)
I've seen picture of algae production systems where there's a pond with algae floating on top, but I've also seen more conventional stirred suspension bioreactor tank systems that resemble what would be used for bacteria, CHO cells etc in biopharma applications!
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Shalini Gupta
Samridhi Rana I see! Thank you all for the insight!!
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I'm troubleshooting Fv/Fm measurements on dark-adapted snow algae samples in the field. We have a field season coming up in the North Cascade Mountains here in Washington State. I have an Opti-Sciences OS1p flourometer. If you have insight into the process, I would love to hear about your successes and struggles.
The unknown factors I forsee coming up are:
- Does the probe need to be submerged in the sample?
- What is the ideal "slushiness" of the sample? i.e. how much water vs. snow?
- How long to dark-adapt samples?
- How much algal tissue is necessary for accurate readings?
Thanks for your thoughts.
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Thanks for the thoughts! I agree that the PAM technology is deceptive at first. It's easy to get a number. The trick is getting those numbers to actually mean something.
I have a phytoplankton cuvette that our optical probe rests on, so maintaing distance from the sample is feasible. We have a bloom starting up just this week, so we'll put it to the test in a couple of days!
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I know different people will have different answers depending on their backgrounds.
Think in terms of Chemicals for a biorefinery
Initial value
Agriculture
Carbon
Transportation
Integration of the mass culture into the economy
Bioeconomy
life cycle assessments
etc...
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Promising biofuel should have higher calorific value, volatile matter, fixed carbon, and higher carbon and hydrogen content. The moisture content, ash content, oxygen, nitrogen, and sulfur content should be as low as possible.
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I have some green algae and red algae in Extract form and due to some reasons I can't use now, so I want to preserve that extract..
so anyone is here who worked with algae then please suggest to me any preservative. I Need That.
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Adding chemical preservatives will certainly confound your evaluation of antimicrobial efficacy. Freezing may be your best option but be aware - that too can affect chemical stability and solubility.
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Hi I am growing marine algae however i have lots of contaminants in my samples such as rotifers . I have attempted to remove these with sodium bicarbonate however the eggs still do remain. i have tried filtration with a 0.45um filter however the filter cloggs. Any suggestions on how i can remove these without losing the algae as the aim is to have a pure culture?
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Thank you all .@md.Hamidur Rahman I am currently undertaking my masters project based at ushaka seaworld if I am successful then i would look at commercial.
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I have to prepare a mixture of 7 antibiotics and test its toxicity on algae. My intent is to evaluate the toxicological effects in 8 different mixtures of antibiotics in which the concentrations of each compound will be arranged in a geometric series with a factor of 2.
Ex.
Ant: Ant1, Ant2, Ant3, … (mg/L)
Mix1: 10, 8, 12
Mix2: 5, 4, 6
Mix3: 2.5, 2, 3
...
My question is: it is possible, and make sense, to derive the value of EC50 for each individual compound?
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Try to separate the compounds and test each one alone, or the determined LD50 is LD50 of the mixture.
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Below ground ecology or below ground ecological functions and ecosystems roles are very crucial and highly significant for any ecosystem especially for the forest ecosystems. The interaction among soil, microbes(fungi, algae, bacteria etc.) and plant roots create the most suitable environment to survive where growth and productivity is more likely to be associated to above-ground factors
Microbial activities, mycorrhizal association, soil amelioration due to saprophytic organisms are an integral part of forest ecology .
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Manoj Chandran Sir, Is there any work prevailing on this from the Indian Subcontinent? I'm interested in this topic.
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We're working on identifying microalgae and macroalgae strains and we plan on doing a PCR later on but we need to exract the DNA first and i can't seem to find methods that don't require CTAB, Liquid nitrogen and phenol:chloroform:isoamylalcohol. We currently do not have these components in the lab.
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hey, Salma Chahid I think we are in the same university. I can share those compounds with you. P.M if you are interested
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I am DO levels in stagnant waters from a stream mesocosm experiment. During the non-flow periods, DO saturation measurements are reaching maximums of 300% (26 mg/L, 21 degrees C). The flumes contained high amounts of filamentous algae and high light inputs, which might partially explain these values. Nevertheless, we are afraid that oxygen bubbles might be generating an artefact.
We are wondering which are the maximums levels recorded by other researchers in similar conditions?
Thanks all for your help and time,
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Hi Gregoire,
Thanks a lot for the information and the links! Really helpful!
Cheers,
R
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I need some help with one observation of mine. I observed a pool of water full with algal blooms. The algal blooms of the pond appeared red in the afternoon but in the evening it turned green. What could be the possible reason behind this?
Thanks and regards
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Some green microalgae species have the protective red carotenoid for high light intensity during daytime (sometimes because of the nutrients content in water like salinity increasing when evaporation take place). When the microalgae get into the ideal condition, they will be in healthy green colour again.
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In my project, we are trying to mutate algae with artificially zeaxanthin accumulated.
I want more meaningful project, so I hope to apply bioinformatics method.
The abstract I think is if I get mutant algae with zeaxanthin accumulation, I will compare some other organism naturally posessing many zeaxanthin with that sequence.
Eventually, I can get which sequence will determine the zeaxanthin accumulation.
But it is my first time to sequence 'DNA', I am not sure how to start it.
Could you recommend me some idea or method about it??
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Hello, Lee Ha Jung.
You can use the EBI server to run some of the well-known tools to perform multiple sequence alignment.
Here it is:
Regards.
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I would like to ask some help if someone can please recommend a precise method in extracting Chloroplast DNA from algae or specifically from Alexandrium tamarense.
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Nice one dear, A better way to get research problem solved