Aging - Science topic

We are a Chinese education company dedicated to helping our clients publish SCI, SCIE, SSCI WoS indexes quickly. We have a wide range of paper orders in various fields and we are actively looking for partners who can help us with our publishing work.

Please note that your service fee will be paid after acceptance and it is necessary for you to share your login information with me.

This precaution is taken to avoid any potential fraudulent activity, as unfortunately there are many dishonest people in the market and I am reluctant to work with them.

If you meet the above requirements, please feel free to contact me

Epigenetics refers to the study of changes in gene expression that do not involve alterations to the underlying DNA sequence. This field has garnered significant attention for its potential to influence aging, combat diseases, and mitigate unwanted side effects of genetic engineering.

Aging is associated with various epigenetic changes, such as DNA methylation and histone modifications, which can lead to altered gene expression and contribute to age-related diseases like cancer and neurodegenerative disorders. By targeting these epigenetic modifications, researchers believe it may be possible to reverse or slow down the aging process. For instance, interventions that modify epigenetic markers could potentially restore youthful gene expression patterns, thereby improving cellular function and longevity.

Epigenetic therapies hold promise for treating a range of diseases. By understanding the specific epigenetic alterations associated with conditions like cancer, researchers can develop targeted therapies that either activate or repress certain genes without changing the genetic code itself. This approach could lead to more effective treatments with fewer side effects compared to traditional genetic engineering methods, which often involve irreversible changes to the genomeOne of the significant concerns with genetic engineering is the potential for unintended consequences, such as off-target effects or the activation of harmful genes. Epigenetic modifications can provide a more flexible approach to gene regulation, allowing for temporary changes that can be reversed if necessary.

This flexibility could help in fine-tuning therapeutic interventions, reducing the risk of adverse effects associated with permanent genetic alterations.

In my view, the most accurate and practical theories in any scientific field, particularly gerontology, are heavily influenced by temporal, spatial, and cultural contexts. It is challenging to propose a definitive theory on gerontology, as the perspectives and experiences of elderly individuals vary across different decades.”

I write about ageing in these 3 articles:

*

*

*

Do let me know your thoughts.

Kind regards

Tiago

Yoga and being vegetarian has great impact. Its also proven. Further if a person sticks down to a routine work and maintains proper diet , it can be controlled. Further natural therapy increases youngness.

Hello Bree Barand

Yes, you are right. Animal cells increase in size as they age. Cells experience changes with aging. As cells near senescence, proliferation slows, but cell growth or the addition of cell mass continues relatively unchecked. The end result is a gradual increase in cell size with age. Old mammalian cells are often two or three times larger than young cells.

Attached below are a list of references that may help.

Best.

Could it be that cells with different ages express different levels of laminin-1? On this paper DOI: 10.1002/aja.1002030404, I have found that "Different temporal patterns of laminin alpha 1, beta 1, and gamma 1 subunit chain expression were observed" ...

Dear Doctor

Go To

Human population growth and the demographic transition

"ABSTRACT

The world and most regions and countries are experiencing unprecedentedly rapid demographic change. The most obvious example of this change is the huge expansion of human numbers: four billion have been added since 1950. Projections for the next half century expect a highly divergent world, with stagnation or potential decline in parts of the developed world and continued rapid growth in the least developed regions. Other demographic processes are also undergoing extraordinary change: women's fertility has dropped rapidly and life expectancy has risen to new highs. Past trends in fertility and mortality have led to very young populations in high fertility countries in the developing world and to increasingly older populations in the developed world. Contemporary societies are now at very different stages of their demographic transitions.

Global population growth will continue for decades, reaching around 9.2 billion in 2050 and peaking still higher later in the century. The demographic drivers of this growth are high fertility in parts of the South, as well as declining mortality and momentum. This large expansion in human numbers and of the accompanying changes in the age structure will have multiple consequences for society, the economy and the environment."

Head and neck cancer management and cancer stem cells implication

Identification and Characterization of Cancer Stem Cells from Head and Neck Squamous Cell Carcinoma Cell Lines

Cancer Stem Cells in Head and Neck Squamous Cell Carcinoma: Identification, Characterization and Clinical Implications

The best and safest artificial sweeteners are erythritol, xylitol, stevia leaf extracts, neotame, and monk fruit extract Md Rabiul Alam

Dear Umair Nisar

Differential voltage analysis has been applied to various lithium-ion cells, to study degradation reactions (side reactions) as a function of cycle number. Standard cycling data is used to calculate either dQ/dV or dV / dQ, which is then plotted against the voltage. In dQ / dV plots, the peaks represent phase equilibria, whereas, in dV / dQ plots, the peaks represent phase transitions. Differential capacity (dQ/dV) analysis allows you to observe what is happening in a battery (including degradation, failure mechanisms, changes in chemistry) in much greater detail than can be observed using aggregate statistics like capacity, energy, and efficiency per cycle.

Conceptually, dQ/dV describes the incremental capacity going into our out of a device over a given voltage increment (some also prefer to use the inverse, differential voltage, dV/dQ). Differential capacity can be derived from raw time series current and voltage data, or accessed directly using a Battery Intelligence platform such as Energsoft. The differential capacity plot displays dQ/dV on the vertical axis, plotted against potential on the horizontal axis, with the loop in this plot showing one charge-discharge cycle.

dQ/dV Differential capacity curve Where there is a peak on the curve meaning the charge and discharge curves have a voltage platform, and different peaks represent different electrochemical reactions.

Have a look at the following RG links.

U have to extend the aging duration by using RTFO, and correlate the data to define how long it should be extended.

Hi Anthony L, Yes: we often used the DNA-damaging drug etoposide to induce senescence. Soluble in DMSO. 10 uM was often ok, though the best concentration can vary with the cell type -- it gets toxic at higher concentrations (because extensive DNA damage is toxic). It was added to recently plated cells for 48 h, then removed, and the cells were left for 5 days to develop the senescent characteristics -- although this may also vary with cell type. So for a new cell type you might want to try lower and higher concentrations, and different timings. We published this method (used as a positive control) in Cairney CJ et al. 2017, PLoS Genetics. (See Supplementary File 2.)

NASA Ames Prognostics Center of Excellenc :

(published 12 November 2020)

Dear Mostafa Shaban

Many thanks for this. This is really useful!

P.J.

It feels great to share it. Hope you find it useful P.J. White

The Society for Integrative and Comparative biology is actually taking more abstracts until October 28th, 2020. And, we are still looking for people to participate in the complementary session to our canine science symposium, so your work is still most welcomed!

To submit your abstract, please go here: http://sicb.burkclients.com/meetings/2021/abstracts/late.php

Hello Tahir, can you please explain how you tested for occupation etc in AMOS?

Please take a look at these useful RG links.

Thank you, colleagues! Eventually I managed to fit the model by the algorithm described in the Appendix B to Ricklefs (1998), thanks to my mathematician friend Igor Sizov who made a working example of this tricky thing so that I understood how it is calculated. If you're curious, see the fitted curves in the attachment. Blue colour are males, red colour are females. Solid lines are the curves fitted by NLS (non-linear least squares), dashed lines are the curved fitted by ML (maximum likelihood).

It is clear for me that NLS curves better fit to the data, while ML curves overestimate the lifespan and do not pass through "older" points. Both procedures use unweighed points. If we use weights, both methods fit worse, and begin to ignore right side points since there are fewer number individuals in older age.

So, think that further I will focus on the unweighted NLS method.

Cheers!

i want to assess the attitude of my young university students attitude towards aging. that is why i am highly interested towards your standardized scale.

@ Liesbet De Bosschere

Please have a look at the following RG links.

Thanks!

Maybe this: could be a good entry point for finding the most appropriate way to solve this.

Thank you all! I have looked into senescence in cultured cells, and will likely use β-galactosidase (or possibly another factor for which I can borrow an antibody) for my long-term cultures.

I am interested in looking at mTOR signaling eventually, as well. This is very helpful, as I am the only one currently looking at aging in my lab!

Yes! That's one of his core areas! And Mathias Alemand (University of Zurich) together with Patrick Hill (Washington University in St. Louis, USA) are co-editing a book which will appear towards summer this year entitled "Personality and Healthy Aging".

I have similar question as Xinyi Cho. What is the reference weight a SD rat should reach to consider obese? Or how many percentage of increase in weight compared to the control rats is considered obese?

You would find its to do with agitation in cognitive disorders and those with dementia. Or related to coprolalia. This article provides an interesting insight:

Plus, I find for it to be classed as a genuine mental disorder there would have to be classification and information upon the current 5th edition of the Diagnostic and Statistical Manual of Mental Disorders.

yes i mostly used for more information you can see

This may be helpful....

It's a contentious question if consider it in human for which there is almost no evidence. In animal models there are certain reports, especially in Zebrafish. Just a lab have published a preprint about it. https://www.biorxiv.org/content/early/2016/06/08/057893

Christa,

Well, it's really a problem. I also asked a couple of companies but none of them has old DBA/2.

I'm just thinking probably we could collaborate. I need hematopoietic stem cells from bone marrow which you might not need. Or, if it works for you of course, is it possible that I buy a few aged DBA from you if you don't need them all...?

As you know, it takes time to age them by ourself so we don't really want to do that. I would appreciate it a lot if you can help us!

Daozheng

My email: d.yang@umcg.nl

I do really agree with David James views. You question revolves around yield regulation. There are some published works that can provide you some insights. However, its applicability depends upon the edapho-climatic regions of your country.

Hi,

Living systems is a result of thousands of consecutive biological reactions, catalyzed by specific enzymes, in orchasetrated manners. NAD/NADH & NADP/NADPH are very important biological cofactors, participating mainly in numerous cellular oxidation/reduction reactions, as an electron acceptor(to be reduced) or donner(to be oxidized). There is another biological cofactor known as FAD(Flavine Adenine Dinucleotide) also participates in biological reactions. And the particular enzyme involved in each reaction, has strong choice (specificity) for its cofactor of either NAD or NADP or FAD, to catalyze the reaction optimally. This specific choice for cofactors of the catalyzing enzymes, makes other analogues unsuitable for biological reaction. The specificity depends on many factors as three dimensional conformity of the subustrates, enzymes, cocfactors and redox(reduction/oxidation) potential of the reaction & reactants etc.

Although there may be some artificial or synthetic analogues that may somehow support a biological reaction in lab conditions, the reation rate may not be optimum as in biological system. So, if cellular reactions do not run optimally, there must be some traffic congestion, thus making the whole system go-slow or idle (ageing?). All These make NAD/NADP so important. Good luck for you.

Hi Sina,

There is the "DNA methylation clock", named "epigenetic clock": the degree of methylation of few CpG loci reflects the biological age. It keeps track of the "solar years", except for many diseased tissues which appear "epigenetically older" than healthy tissues, and has a different pace in different tissues.

Two reviews about this question: PMID: 25913071; PMID: 25341512. There are many recent papers about the epigenetic clock but not that many reviews. One founding paper: PMID: 24138928.

Dear Researchers

Thanks for the paper suggestions by you. Went through all of them and also a few more which took me a time of a couple of months to write back to you.

The idea developed from the papers are useful and quite interesting.

The mTOR pathway is not related to mechanism of natural aging, therefore it unable to be the basis for elaboration of radical remedy for senescence (see: Trubitsyn A.G. “The Lag of the Proliferative Aging Clock Underlies the Lifespan-Extending Effect of Calorie Restriction” Current Aging Science, 2015, 8, 220-226.) ;�};��Q*G��ȵK�9

The probability depending on the environment which is always diverse will undoubtedly create suspicion/paranoia/ and habit forming points of consumption where gateway and comfort leads to forensic psychology on a clinical and enhanced therapeutic level.

I believe there is a genetic component here. I found what I was looking for - thank you!

Hey Nazli,

It would depend on what polymers you are using but thermoplastics above glass transition temperature will undergo alignment of polymer chains when placed under load. This alignment strengthens and stiffens the material (in the direction of the load) and is used with some polymer threads to improve their mechanical properties. This process may be occuring with your samples with the first contractor.

Mat

i send you message, and lets start to work on this research,

I agree with Ron's assertion that aging is, to some degree, linear. It may nevertheless be possible to progressively increase activity throughout up to a point. Imagine, activity is increased slowly through one's 40s to 80s, e.g. then, life happens and an illness or injury impairs the individual's capacity to remain active and there's a sharp drop-off that persists over the remainder of their life. If that remainder is very brief, then sure, the goal of increasing activity throughout one's life was met. At the very least, it could be shown that activity was progressively increased more than had previously been demonstrated.

Thank you for your contributions.

Hello António Alberto da Silva Francisco 

please check the resources related to your topic

Looking forward to reading your paper Beatrice

Once the shock absorber seal gets damaged due to longer usage of shock absorber, the oil will come out. Otherwise the shock absorber will have maximum age

dear Aditya,

what are the specific temperatures ? do you apply any stresses? do the twin dislocations disappear at all conditions?

If you observe a significant grain growth it could be the cause for the twin reduction, since also the growth means a total energy reduction. As stale dislocations require a certain volume of material (max. dislocation density) a shrinking grain with a dislocation in it will contribute to further grain growth, respectively cause dislocation silp at some Point when the local energy reaches a certain Level.

Thank you Igor Kurochkin for the database. In this one you can select people without dementia, which is what I want. But you do not have the young people expression unfortunately.

Biomarkers? There is a rhenium-osmium clock

for bitumen. Carbon dating goes back only

a few thousand years.

Regards,

Joachim

In perennial trees, we usually measure the thickness of both scion and rootstock to see the response to inputs and scion girth just above the bud union will give the age of the trees, which comes through experience , not a concrete method at all...Dr Anshuman Singh ..

The question is quite old, but the answer for this question is yes. With simple tools the primary care physician may screen for frailty, and it is not very diffucult to perform. When we talk about frailty, we all know that frail older person you may easily recognise. As a nurse, I may point out that not only physician may screen for frailty,it may be done also by other health team members, eg. nurses.

Thank you so much!

I have not seen the article. If well written, it can be publishable, I can only pronounce that if I read it. 

Dear Youcef,

In general, the loss of stabilizer due to thermal ageing increases the conductivity of the PVC cable insulation. We had several investigations and publications in this filed. I think the attached is a good summary.

You also need to be concerned about the alignment of fMRI data to atlas space. Make sure that you generate a template that is representative of both younger and older adults. You can get group differences simply due to better spatial overlap of the signal in younger adults.

For a paper that touches on spatial overlap.

Another paper on neurovascular coupling

The best should be the hs-CRP which brings more information 

Six months

Hi Touqeer,

Some of the most expensive components of an in vivo and in vitro setup is the anti vibration table. You can avoid buying such system by putting a table, prefereabily a metal one and just the square stuff, over an old big truck tire or over four half tennis balls in the corners. The metal will help because most of the micromanipulator bases can be attached to the table with a magnet, helping to stability and giving flexibility in location. The table should weight enough to provide stability and bend a bit the rubber. This option is used in labs even for single-cell patch clamp recording and should provide stable recording conditions for extracellular field potentials both in vivo and in vitro.

Hope these solutions help!

Kind regards,

Marcel 

are we talking about ADs to older consumer or the use of ancient ads, repeated ad nauseum through diverse TV channels? For example, Mars via its Galaxy (Dove) chocolate ad delights in repeating the same old AD over years now. For me, the outcome is a complete turn-off for Galaxy itself, and an annoyance with Mars who do not seem to understand too frequent repetition of the same AD drives customers away from - not toward the brand.

Dear Soheil,

The best way to calculate thermodynamic parameters is TermoCalc software.

Yes, if you provide the brain region and the protocol of LFS you are using it will be easier to help you. Are you trying to induce LTD chemically and electrically?

Anyway in my experience LTD (I mean electrically, in cortex and in vitro) is not always easy to obtain, you need really a lot of patience....

My LFS protocol is: 900 stimulus pairs of 0.1 msec (50 Hz, i.e. 20 msec interval)  at 1 Hz.

I do not disagree with the opinions above, but motor behavior and weight can vary significantly even in inbred strains. Although some favor inbred strains for genetic homogeneity, it may also make results less generalizable and can occasionally produce unusual GxE interactions.. This is why we do clinical trials in humans with diverse genetic backgrounds.  When backcrossing, you may also find that fertility or pup survival suffer greatly.

Aging and BBB dysfunction is an interesting topic because its a high risk factor for developing neurodegenerative diseases. To my knowledge most of recent studies point at loss of tight junction proteins of the BBB with aging which is likely due to neuroinflammation. Infiltration of circulatory leukocytes and blood borne immunoglobines into the brain is a marker of BBB leakage that happens in aged humans. In aged mice however, only loss of TJ proteins of BBB was observed. I think its not clear which come first, neuroinflammation or BBB leakage. It could be systemic inflammation disrupts the BBB first and lead to infiltration of toxins to the brain that trigger neuroinflammation. Not sure if the brain itself with aging would autonomously initiate inflammation due to certain malfunctioning in metabolic processes and clearance pathways to remove waste byproducts !?.. I would love to here comments from experts in the field..

Recommended in 18-65 year olds. Not validated in older groups. So there are no normative values.

Smith MV, Calfee RP, Baumgarten KM, Brophy RH, Wright RW. Upper Extremity-Specific Measures of Disability and Outcomes in Orthopaedic Surgery. The Journal of Bone and Joint Surgery American volume. 2012;94(3):277-285. doi:10.2106/JBJS.J.01744.

I can't see the relevance of this study. If the client has the motivation to eat and the skills involved - what is the significance of grip strength after meals? How will your study provide information that is meaningful to clients healthcare outcomes? What are the assumptions/hypotheses underpinning your study?

Perls staining is pretty effective, easey and low cost.  We used it here.  The colour contrast is pretty good for image analysis.

Again, I am no expert but the way I understand it is that children are more susceptible to the effects of ionizing radiation.  They are still growing and developing and their cells tend to divide more rapidly.  During cell division there is an increased risk of mutation especially when external influences such as ionizing radiation play a part.  Also, children have longer to develop an accumulative effect than adults. 

 Dear   

Larissa Donatoni da Silva

National University of Ireland, Galway

   I am of the opinion that instead of asking for valid questionnaire it is better to develop it yourself by studying your own problem or project with which you will be dealing. The questionnaire developed can be tested and retested to increase its validity. This is bit laborious but it also trains up you. Yes you can ask for help and many interested and experienced people will do this.

I haven't published on this, but I have found in my clinical work that older people tend to be more comfortable on the whole with the idea of their own death, though perhaps not of the act of dying. They may fear suffering. They also may be less comfortable as they age with the deaths of friends and other family as their key networks then shrink. Those who live long are "successful" through their long life, but at times find it emotionally difficult to outlive their support systems.  Some evidence below.

Victor Cicirelli has worked in this area for a while.  Two of his publications are:

Cicirelli, V. (2011). Religious and Nonreligious Spirituality in Relation to Death Acceptance or Rejection. Death Studies, 35(2), 124-146.

Cicirelli, V. (2003). Older adults' fear and acceptance of death: A transition model. Ageing International, 28(1), 66-81.

This article below suggests that older people are more comfortable with death:

De Raedt, Rudi, Koster, Ernst H.W., & Ryckewaert, Ruben. (2013). Aging and attentional bias for death related and general threat-related information: Less avoidance in older as compared with middle-aged adults.(Author abstract). The Journals of Gerontology, Series B, 68(1), 41.

Hi Rick, can you please share the FG-NET Database with me? and MORPHII if you have. i am doing a research on facial age estimation. thank you.

You can control systematic error with the proper model of sequence evolution - use some model choice program to help you identify the best model. However, because sequence evolution follows a Poisson process, the random error (standard deviation) with increase with time back to the common ancestor (mean) and will always be quite large (a Poisson mean equals the standard deviation), Nevertheless you do have control over reducing the systematic error so do that at least.

DNA methylation age and telomere length have only a weak negative correlation after you correct for chronological age. As an aside I mention that it is important to correct for chronological age before one relates DNAm age to telomere length because chronological age confounds the relationship between the two variables.

We correlated DNAm Age with telomere length in *adipose* tissue (Horvath et al 2014, PNAS, Title: Obesity accelerates epigenetic aging of human liver") and only found a weak and insignificant negative correlation between the two variables after correcting for chronological age (e.g., r = −0.28, P = 0.22). We and others have seen similar correlation coefficients in blood tissue but these appear to be unpublished.

Bottom line: the epigenetic clock relates to a biological process that is largely independent of telomere attrition and cellular senescence. 

In the language of multivariate linear models:

if you fit the following regression model:

lm(DNAmAge~AgeAtBloodDraw+TelomereLength)  then TelomereLength will only have a marginally significant p value.

Conversely, if you fit

lm(TelomereLength~AgeAtBloodDraw+DNAmAge) then DNAmAge will only have a marginally significant p-value. Caveat: bmulti-collinearities between the 2 covariates will make the coefficient estimates unstable.

Best,

Steve

Hi Allaudin

Breaking toys after playing with them for few days may not have any hidden psychological predisposition except the 5 to 10 years old's curiosity in exploring the functioning of the toys! beacuse since they have already played with them for few days they might be exploring further possibility and in the process ends up breaking them

regards

Rathish

Have you seen this? Ted Garland, 2015. Diet-induced_obesity_resistance_of_adult_female_mice_selectively_bred_for_increased_wheel-running_behavior_is_reversed_by_single_perinatal_exposure_to_a_high-energy_diet?

P.S. I wonder if the question you ask has been addressed using monkeys by anyone, say, at the Oregon Primate Center? They do a lot of work on early nutrition (including prenatal) and diabetes risk. 

It depends which aspects of aging you're most interested in...