Adhatoda - Science topic

PLEASE IS THAIR ANY WEBINAR TO LEARN AMIX SOFTWARE

Have you find any answer for your question? I would appreciate if you could share it with me.

Greetings Dear Huda

  Please, press on the following website link for more information.

With my good wishes

 It is difficult to identify species of Oryzias from photographs, as some internal characters are needed; I would suggest that you use the key of Parenti (2008: 542-544) for identification.

Parenti, L. R. 2008. A phylogenetic analysis and taxonomic revision of ricefishes, Oryzias and relatives (Beloniformes, Adrianichthyidae). Zoological Journal of the Linnean Society v. 154: 494-610.

happy to your honorable visit

I was wondering if the data were from, say, a home soil test kit --- similar to water test strips --- that have a colorimetric reaction that is interpreted only in rough numbers.  For example, for a water test strip for a "high range" nitrate test the values might be 0, 1, 2, 5, 10, 20, 50 mg/L.  If you are using this for, say, unpolluted waters, it is easy to get all values of 1 or all values of 2.  And even then, it can be difficult to determine between a value of say 1, or 2, or 5. For an egregious example, see https://i0.wp.com/preclaboratories.com/wp-content/uploads/2016/10/NAT-Nitrate-test-strip-label.jpg .  Some kits use multiple color spots on the strip to improve the precision of the measurement. And some have a choice of "low range" or "high range" kits.

In some cases, this data is of little value from a statistical point of view.  Practically, it may be useful, perhaps, to know that the nitrate in the lake is about 1 or 2 or 3.5 mg/L and not say 10 or 20 mg/L.  But statistically, the analyst may not be able to be more precise than saying "it's in the 1 to 3.5 color range."

If the data are of this type, caution should be used in doing anything statistical with the data, even treating it as categorical data.  For example, imagine measuring soil nitrate, and using a scale where < 10 mg/kg is insufficient for crop yield, >=21 mg/kg is sufficient, and in between is in between.  Maybe the the way you are measuring the soil nitrate, the values are being reported as all 25 mg/kg.  But maybe in reality the analyst would be unable to distinguish values corresponding to 10 or 20 or 25 mg/kg.  In this case it would be wrong to conclude that the test indicated all the soils had sufficient nitrogen.  In fact, this method of measuring soil nitrate would not be useful in this context.

20

Why cant it be Rutaceae?.  Check for Glycosmis species

thanks very much mam

I mean it is not possible - you have no standards for RT and mass and have no exact mass at your system (what about isobars?). You may work with ramping the collision energy and compare ramped spectra, if they are in library - it is real proof, but we use only standards - it is better, because in special cases for our analysis without exact mass MS/MS/MS on the trap is necessary.

Essentially all chemicals (synthetic and natural origin) receive a CAS Registry Number. In this case, these compounds can be considered as "known". With this number, you can make database searches, in which natural sources this compound has been identified already. One of the largest databases is the Chemical Abstracts Service with their Scifinder software, a commercial product of the American Chemical Society.

The terms "crude" and "true" are not scientific terminology but a sort of slang. Generally a "crude" protein refers to a mixture of the protein in question and other proteins: meaning an impure preparation of the protein. "True" protein is not a term with which I am familiar. A protein preparation can be "pure" meaning it is homogeneous in that protein and contains no other measureable contaminating other proteins. It is possible that other different proteins are still present but at concentration levels below that can be detected with available measuring procedures.

Hoping this will be helpful to your project

Hi Nelson! The code I used (previous post) solved it! It works!

The idea of making column names as numeric can be interesting. I will save this piece of code you sent and keep it in mind for another time! Thanks for the help man! 

Ok... Thanks Tuhin

yes ,i agree with bruce 

Dear Dr. Keekan,

Firstly, if you know the molecular weight and solubility of your compound, you should prepare a standard solution of 1 ppm from your compound in the suitable solvent and make manual tuning to get the optimum conditions for MS parameters and LC parameters with suitable mobile phase.

Secondly, if you don't know any information about your compound, you should inject it on scan mode and try to get the molecular weight of it and its mass spectra to identify it.

with my best wishes

M. H. Abdelwahed 

Dear Pushpak,

Dr. Tapan Seal <kaktapan65@yahoo.com> of our institution works on these aspects. If you like you can contact him.

Yes sds-page is probably the cheapest and easiest way. 

1. Use very variable extracts of of the plant of interest (different origins, plant parts, developmental states, extraction solvents etc.).

2. Measure bioactivity and LC-MS profile of each extract.

3. See which peaks are correlated with bioactivity, for example by using PLS.

4. The peaks should be isolated to confirm their bioactivity and their identity.

with a minority product you will have to screen a lot of colonies but you can mix colonies in each pcr reaction . say 5 colonies  per pcr reaction then any pcr showing 2 bands the mixed colonies can be replated and re screened.No purification of dna is necessary the colonies just lyse and amplify

Thank you Dr. Misra for putting your effort and time to write me in such a nice detail.

Look like the genus Cyclops.  As to species, there are over 400 so you will need a taxonomic key.

Order: Cyclopoida

Family: Cyclopidae

Genus: Cyclops

Pyrrolizidine alkaloids (PA) are a common contaminant in teas, herbs and even honey. Check out these papers:

and a good review: 

'Pyrrolizidine alkaloids: occurrence, biology, and chemical synthesis' in Nat. Prod. Rep., 2017, 34, 62

https://www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)