Abscisic Acid - Science topic

What role does abscisic acid play in regulating transpiration and water loss during drought stress?

Is it that chlorophyll content and protein level are significantly affected under drought?

Do drought-tolerant genotypes produce specific secondary metabolites? If yes what are the secondary metabolites?

What should one look for (in terms of biochemical parameters) when studying drought tolerance or sensitivity in legume or cereal genotypes.

Please see the link below:

Is there a text book or paper that explained what happens after corn receives exogenous abscisic acid (ABA) foliar application, how it's absorbed and transferred? where does it go and what does it do? what are the mechanisms and what changes happens in the plant (up or down regulations for genes, biosynthesis pathways etc) that lead to increase root hydraulic condtivity, stomatal closure and so on?

Methods that can help to find out the plant ABA content under abiotic stress.

I have gene analysis experiment in Arabidopsis thaliana using qRT-PCR, I'm wondering which reference genes i can use knowing that i subjected the plants to abiotic stress ( abscisic acid, H2O2, HNE ( 4-hydroxynonenal)) Thanks in advance

I'm studying the levels of abscisic acid in a few designated plants. It’s widely accepted that (S)-(+)-cis-ABA is the naturally occurring form in plant. However, I wondered have anyone experimentally identify R-(−)- abscisic acid in plant. Much appreciated if someone can share their insights on this.

Regards,

Wong

I want to Quantify plant hormones (ABA, JA and SA) by LCMS/MS.In most of the hormone extraction and quantification protocol  isotope labelled standard of the corresponding hormones are used as Internal standard in LCMS. However in my case I don't have isotope labelled Internal standards. I have only normal standards of the hormones. So, In such a situation, can I use Leucine Enkephalin as internal standards for the Quantification of plant hormones (ABA, JA and SA) by LCMS/MS. Actually I am saying so in reference to a protocol which I got in internet and they suggested to use Leucine Enkaphalin as internal standards for the Relative Quantification plant hormones (ABA, JA and SA) by LCMS/MS.

Reference Link

Does anyone have a list/a link to a publication with a list of the best solvents for ABA, specifically S-(+)-ABA?

Do you know any example where abscisic acid or salicylic acid induce tissue-specific or age-specific transcription factors?

Abscisic acid hormone can be estimated by HPLC on dry tissue not a fresh tissue of maize leaves??

Abscisic acid (ABA) concentrations in tissues.

Hello, everyone. Recently I performed an experiment to investigate the dynamics of stomatal movement, guard cells on the intact leaf were applied with ABA (Abscisic acid) and monitored the stomatal aperture by the camera. The stomata started to close after the treatment. I obtained the stack containing 100 images. Now I want to analyze the stomatal aperture by Fiji/imageJ. General speaking, most of people use the “straight line” function to measure it. But the stomatal aperture changed all the time, from large to small when the guard cells are given ABA. That means we need to adjust the length of the straight line by hand all the time. It is inevitable to make an error that leading to an unreliable result. So, does anyone know how to measure the dynamics of stomatal aperture automatically by Fiji/ImageJ?

I'm studying the expression of some genes involved in drought response. I would like to set up an experiment in durum wheat in which plants will be treated with different concentration of exogenous ABA.

Is it necessary to use the (+)-ABA or I could use the (+/-)ABA for the treatment for this purpose?

Regards

Valentina

Could anyone suggest a protocol to estimate Abscisic acid (ABA) in plants using spectrometer ?

Is there a simplified but accurate measurement by spectrophotometer?

Our HPLC instrument has C18 column and gets choked as soon as we use any buffer system to maintain pH so we cannot technically use any buffer or acid for lowering  pH. 

I need some HPLC method for analysis of vitamins without using buffer. 

I need procedures to quantify ABA in wheat leaves. But we doesn't has a HPLC/ GC-MS, so may be there is some alternative methods, it will be useful.

Thanks

I am doing so R&D on role of Abscisic acid to improve coloure & texture in grape, so please share some knowledge about it.

This is for PCR amplification.

My understanding is that ABA is more sensitively detected under negative mode (263>153), but I failed to obtain any peaks from my standard and samples. Under positive mode, on the other hand, we have got a peak from both the standard and samples (265>247). Since the majority of publications are showing data of ABA analyzed in negative mode, we are also eager to do so.

Why do you think we are failing to obtain results in negative mode? What are the possible reasons for getting results only from positive mode but not from negative mode?

Thank you very much

Xanthoxin is a part of ABA biosynthesis. Is there anyone who has a protocol for its quantification?

I am looking for d6-ABA standards and also looking for the supply of ABA analogs listed below;

 ·         (+)-8'-acetylene ABA

·         (-)-2',3'-dihydroacetylenic abscisyl alcohol

·         (+)-8[prime]-Methylene ABA

·         RCA-7a [l-(3-carboxyl-5-methylphenyl)-l-hydroxy-2,6,6-trimethyl-4-oxo-2-cyclohexene]

·         2',3'-dihydroABAs

·         (+)-(2Z,4E)-(1S,6S)-5-(1-hydroxy-2,2,6-trimethyl-4 oxocyclohexyl)-3-methyl-2,4-pentadienoic acid)

·         Methyl (me)-ABA

·         ABA aldehyde

·         Dihydro ABA

·         Acetylenic divinyl me-ABA

In stomatal activity, does ABA has transcriptional effect or just only the ion channels?

What is the difference of ABA function in stomatal activity vs. seed germination?

How does ABA react when GA is present during germination?

What are the precursors of the major plant hormones (auxin, gibberellin, cytokinin, ethylene, and abscisic acid)? What are the major genes that control/interact with them?

I have my leaf samples already freeze dried and stored in the silica desiccator, for some of the samples have been ground into powder and stored in glass bottles at room temperature. I am going to use these samples to measure free sugars, proline, MDA and abscisic acid, is this the proper way to store the samples?

Thanks. 

Protoplast extraction, plant hormones, mechanical injury induced hormones. Assays on cellular levels.

Is it possible to carry out plant phytohormone (Jasmonic acid, Abscisic acid, Salicylic acid) Quantification in LCMS/MS without Internal standard?

And if yes how far the quantification is acceptable?