Questions related to Abiotic Stress Tolerance
I couldn't see much paper where plant breeders use biochemical such as proline content Malondialdehyde (MDA) and dyes such as NBT or DAB( for ROS detection) for screening stress-tolerant accession on a large scale (100-200 which I suppose is possible to do). Are not these methods better than phenotyping grain yield, biomass, plant height, NDVI, LAI, etc?
Is it that chlorophyll content and protein level are significantly affected under drought?
Do drought-tolerant genotypes produce specific secondary metabolites? If yes what are the secondary metabolites?
What should one look for (in terms of biochemical parameters) when studying drought tolerance or sensitivity in legume or cereal genotypes.
Please see the link below:
How to find the candidate genes to validate their role through functional genomics experiments such as cloning, transgenics, over-expression, localization, and its interaction with other proteins and DNA, etc.
1)Do we need to study a lot of literature and see which genes role is not deciphered in particular traits e.g. drought stress?
2.) Do we need to perform our own transcriptome or comparative genomic studies or analyze already published studies from literature?
3. ) Do we need to perform our own marker traits association(QTLs) study or already published studies?
4.) Some people functionally characterize already known genes(say arabidopsis) to plant of their interest (legumes). But is it a significant or novel research problem to work upon?
5. all of the above.
Many QTLs are being reported in rice for salinity tolerance using bi-parental populations and n number of traits. I am looking for QTLs with LOD more than 5 and variance more than 15-20 %
I believe through scientific development we can create technologies to tackle the climate related problems in agriculture. We have already achieved to some extent, for example with submergence tolerant rice varieties which can tolerant complete submergence. However, the main concern is that the development is not always positive, it also brings some drawback. In a changed scenario the results go in the opposite direction. The materials we develop with so much effort become non-performers compared to earlier ones. Is it possible to develop varieties which can tolerant all sort of vagaries? How?
The duration and intensity of drought is increasing worldwide largely due to climate change.
Development and genetic improvement of drought tolerance crop become very important for the future due the effect of climate change.
What the best method for screening and evaluation drought stress on crop plants especially "rice"?
Thank you for the answer
I need an updated (published in the last 5 years) book clearly and extensively explaining the concepts underlying developmental processes (embryogenesis, flower, root, shoot and leaf development) in plants and how plant endogenous signals and environmental signals are perceived and integrated into the initiation, proceeding and control of developmental processes.
Thanks in advance
Tobacco plants were subjected to 3 different nutritional treatments (A, B, C) for 3 weeks, and also were treated under two irrigation regimes during 2 weeks (Well-watered plants or Control plants, and Water deficit treated plants or drought plants). During drought plants treated with the treatments called "C" showed: (i) better water parameters, (ii) higher growth than control, (iii) higher water use efficiency and water saving, (iv) better recovery from extreme drought, etc.
- Model organism: Tobacco (Nicotiana tabacum L.)
- Pot size: 7.5 L pots (pot size 20 cm × 17 cm × 25 cm)
- Substrate: a mix of perlite:vermiculite (4:6)
- Treatments: Seeds were sown and two weeks later (15 days after sowing, DAS), seedlings were transplanted to 7.5 L pots. Then, plants were subjected to three different nutritional treatments. After 30 days (45 DAS), in addition to the three nutritional treatments, plants were also subjected to two irrigation treatments: optimal irrigation (control; CTR), in which pots containing tobacco plants were irrigated up to 100 % field capacity (3.5 mL g-1 substrate) throughout the experiment, and moderate sustained water deficit (WD), with pots irrigated every two days up to 60 % of field capacity (2.1 mL g-1 substrate) for 20 days (64-65 DAS).
Fresh biomass was collected in each treatment, and the following organic compounds were determined: MDA, H2O2, PROLINE, and PHENOLICS. Also, the PEROXISOME CATALASE activity was determined.
-Malonyl Dialdehyde (MDA) content, hydrogen peroxide content, and catalase activity are cellular oxidative stress biomarkers.
-Proline is a very important amino acid that which accumulation is correlated to plant stress tolerance
- Phenolics (phenolic compounds and flavonoids) are the largest group of phytochemicals that account for most of the antioxidant activity in plants.
In the attached figure there are the results of the ANOVA statistic in CTR or DROUGHT plants, and also is showing the logarithm with the base of 2 of the ratio between drought and control values to understand the decrease or increase in drought plants, in contrast, to control plants for each parameter.
What I see in this result is that there is no clear pattern related to a water deficit regarding the great results obtained in water parameters, plant biomass, water consumption/efficiency, photosynthetic activity, recovery from water stress deficit, etc.
To sum up there's complete nonsense in results in contrast to "C" treatment:
-No changes in MDA (reduction in the other nutritional treatments???????)
-Increase in CAT y H2O2???????
-a decrease in PROLINE????
-An increase in PHENOLIC compounds is logical due to the increase in H2O2, but it has no sense in plants that are more tolerant to drought.
I hope that someone might help me resolve this nonsense
I would like to get more information about naturally occuring osmoprotectants or compatible solutes (eg: glycine betaine, trehalose, sorbitol, fructan) in helping plants to cope with abiotic stress. Has anyone carried out related research or has good information to share?
Scientists are still not getting very much successful in designing stress tolerant crops even after applying conventional ,omics and transgenics approaches to stress tolerance . .What are the main reasons behind it .
I'm going to begin with determining the sublethal stress levels as low and high. The low, middle, and high levels of cold and osmotic stress, and short, medium, long time would be 3 independent variables with 3 levels.
Then I would like to use BBD to choose optimum stress adaptation conditions for the lactic acid bacteria. Does this sound applicable&reasonable?
I wonder whether you guys have included SA (salicylic acid) in your study or not? If yes, what are the methods of its application? will you apply it as a solution through roots or will you just spray it on the leaves? and what are you planning about the concentration of SA?
My apology in advance if SA isn't a part of the project.
we are working on improving salinity tolerance in plants using various strategies. one of the biochemical indicators of salinity tolerance in plants is the synthesis of the amino acid proline.
working on salinity stress and using bacteria but i want to compare the bacteria effect on salt stress and if someone know any chemical that reduce the severe effect of salt and enhance growth in saline soil.
Both Halophytes and glycophytes posses almost same genes that are mainly responsible for salt tolerance and halophyte can easily survive their. Infact the glycophytes contain the all the potential genes necessary for salinity tolerance in their genome and I can't understand why they are not survive.Can anyone clarify me, I'm very confused.
It is well reported that under salinity stress Na ion make harmful effect in crops while potassium ion (K+) maintains the homeostasis under salt stress.
Both these atoms are positive ions and falls under s-block element within same class 1A as per periodic table.
If the chemical features are same for both ions then how sodium make harmful effect while potassium helps for survival of plant under salinity stress.
What is the actual reason for the distinct effect of these two ions?????
I am a researcher working in plant abiotic stress tolerance. Recently, I carried out transcriptome profiling of a drought tolerant plant under salt (150mM) and drought stress (15 % PEG). I observed a large number of ribosomal proteins, translation initiation and elongation factors being downregulated (nearly 390/2500 odd annotated genes) under both stress, specifically in root tissue. Just about 5 genes in this category were DEGs in leaf tissue. It seems to me that there is a massive reduction in translation process in roots in response to stress, but I am not sure what conclusions to be drawn from this, especially with very few being downregulated in leaves
Any help is appreciated
Thanks in advance
we are going to use the PAM-2500 to measure chlorophyll fluorescence parameters in grapevine. The measurements will be conducted outside, therefore we want to keep it simple, but of course efficient in data aquisition. Therefore dark adaptation is no option for us, bu the instrument has many different functions to circumvent this problem.
I have the following questions:
1. the field screen with the Y(II) measurment provides no light acclimation step with standard actinic light, just a light puls. do you think a standardized acclimations to actinic light is necessary?
2. Fo´is measured under field sceen after the light sat puls and a short period of FR. Is this similar to the FR+Y option?
3. do you use default light intensities? we are working with grapevine
I measure 60 barley genotpes for chlorophyll content using spad CCM200 under three conditions control, drought and heat. I found most of genotypes the chlorophyll content under drought and heat is higher than control at flowering time . Do yo have an interpretation for this?
in each treatment
replications = 2
We are working with a number of genes that may confer abiotic stress resistance in Arabidopsis and are interested in any relatively straightforward procotols that we could use to screen Arabidopsis seedlings for tolerance to salt, temperature or osmotic stress.
in my study i found that hoeing treatment affects on cotton root length so i want to know the appropriate explanation for this strange matter.
We would like to identify the possible responsive genes for our target traits (abiotic stress tolerance). For this, we are planning to perform the RNA-Seq experiment. Can you please let me know which program/software (Free Version) will be better to figure out the closest/high responsive genes for our target trait in eukaryotic RNA-Seq data?
Can paclobutrazol (GA-biosynthesis inhibitor), as foliage spray may enhance total biomass in potato grown under heat stress and long days ?
Salt stress is similar to water stress but there are the toxicity of some ions like Na+. My question is how to differ between salt stress and water stress ? I know that water stress is a part of salt stress, but certainly there are biochemical differences . Is the process which make the plant wither is the same? Thanks.
Methyl Jasmonate is an endogenous and volatile compound. What would be the effect of exogenous applied methyl jasmonate on its volatile and endogenous effect?
Is there any related literature focusing on comparing drought resistance between Diversispora and Glomus genus?
Dear All colleagues
I'm researcher from Egypt. I'm interesting with , fruit trees physiology, fertilization , enhancing abiotic stress tolerance,. Recently, I'm collaborate with my colleagues to conduct out several experiments on bio-energetic crops (Jatropha and Jojoba) : micropropagation, cultivation these trees under high density to increase the productivity, and studying effect of some horticultural practices on yielding and oil parameters of bioenergetic crops....Is there someone from CZech to apply with me for Egypt-CZech cooperation program?
If abiotic stress is given for 1 week to any crop plants then wat alterations occur in level of soluble proteins?
The work upon the anatomy and ultrastructure of higher plant in connection to stress is scarce and I need to collect a number of papers in this field.
Under heat stress, in cotton crop, ethylene will produce and elevated co2 with AVG(aminoethoxyvinylglycine help as ethylene inhibitor. Thanks
I am teaching phytohormones to MSc students. For the practical part of the course we conducted some experiments related to the effect of ABA on germination, stomatal closure and leaf senescence. I would like to see whether ABA can be quantified from the extract of salt or drought-stressed leaves using a bioassay protocol. The extract can be the rude extract of the leaf or leaf extract in a buffer. I would be grateful if somebody provide me such protocols.
Quite often it is said that if we write studies to understand the drought tolerance of coconut or some other crop it is not correct. Please clarify this.
I need to find the newest researches in the global for waterlogging stress in different plants?
If it is possible please tell me or suggest the results after 2013 years and specially in Sesame cultivars.
Must we use stress conditions after planting, and on the first day, or should we use stress conditions after 4 days of planting?
Please explain more about your answer.
While abiotic stress which relates to natural factors like soil, climate etc. is difficult to change and manage, it is the types of policies adopted which can make the difference between success and failure. I would like to know what support the social scientists can offer and how. I also would like to know about the various tools and techniques which social scientists can use to help reduce the abiotic stress in aid of the crop/ animal scientists.
We know that proline is related to stress and there are many possible roles to proline on plants. However, I am wondering if high levels of proline in plants with no significant stress could naturally occurr. Perennial plants experience stressful conditions many times during their lives, many times in repeated cycles, then would it be possible produce proline prior another stress time to avoid damage? What do you think?
I want to transfer multiple genes for abiotic stress tolerance to rice. Which vector should I used? Which cloning method will be suitable for multi-gene cloning?
An ABA-insensitive arabidopsis mutant (screened by myself) is that which can produce green cotyledons on MS supplemented with 3, 5 and 10 uM ABA.
4-week old soil grown arabidopsis plants were brought under abiotic stress (salt and drought) for 2 weeks. Less accumulation of MDA and H2O2 was observed in mutants as compared to wild type. What would be the possible reason and mechanism for less accumulation of MDA and H2O2 under stresses?
In most papers it is reported that maximum absorbance of glycine betaine as 365nm, but even after several repetitions I am getting its maximum absorbance at some other wavelength (i.e 200-220nm).
I am working on frost tolerance in faba beans. I have two treatments (after and before frost). I measured the shoot fresh matter and dry matter in these two cases. The correlation between shoot fresh matter before and after frost was positive. Also, the correlation between shoot dry matter before and after frost was positive.
Do you have scientific interpretations supported from other literature?