Abiotic Stress - Science topic

Recently I complete my MS biotechnology and study was focused on abiotic stress and plant physiological response. Know in PhD I want to continue my MS work and explore molecular mechanisms behind physiology response again abiotic stress. In this project I want to screen genes associated with this particular abiotic stress and which metabolic pathways associated with these genes

And want to do my PhD degree with this project in china on CSC or any other scholarship.

So please suggest me the best universities or supervisors having this research topic or interests. Thank you.

I having a problem regarding the formula and calculation of H2O2 content estimation in plant sample. I follow methods of (Velikova et al., 2000).

Can anyone suggest some monoembryonic rootstock of mango with abiotic or biotic resistance?

Dear colleagues,

The registration for the next International Plant Cold Hardiness Seminar is now open. If you intend to join us, please register under:

Even if you are not willing to come, could you please help us to spread the information to anyone who may be interested in the effect of frost on plants from any perspective (eg. physics of ice formation, cellular and molecular response to cold, shift in distribution due to cold temperature, yield loss due to freezing events) in your own community (lab, country, discipline)?

Many thanks,

Guillaume Charrier for the IPCHS organizing committee

For Zea mays up to the 4-leaf stage

For Phaseolus vulgaris up to the fourth trifoliate leaf stage

Factor 1: Abiotic stresses (such as heavy metals, drought, salinity, etc.)

Factor 2: Foliar spray treatment with a plant resistance inducer solution

...

Please need help of all electrochemistry experts.

For example, if a biparental mapping population is screened for abiotic stress tolerance (drought/salt) with physiological parameters.

Biochemical prospects

Hello Researchers,

Definition: a sterile vestigial pistil remaining in a staminate flower

Inference: Most common causal factor for phyllody is abiotic stress, if phyllody is effected by stresses, plant can attend into normal stage after stabilization of hormonal flow in a favorable environmental condition. Phyllody caused by genetic factor has been reported to be inheritable in many crops (Primula, prunus..)

Is it that chlorophyll content and protein level are significantly affected under drought?

Do drought-tolerant genotypes produce specific secondary metabolites? If yes what are the secondary metabolites?

What should one look for (in terms of biochemical parameters) when studying drought tolerance or sensitivity in legume or cereal genotypes.

Please see the link below:

I have a gene of interest (assume X) which plays role in many developmental events in plants. But I am trying to find its role in abiotic stress sepcifically drought stress. I have checked the expression of X in few different tissues where X express abundantly under drought stress (data available in database and reports) which is significant.

I was thinking to explore how X gene controls the photosystem when the plants are under stress. X gene is already reported to be involved in the regulation of photosynthetic genes using trangenics and other interaction assays.

Now, I am stucked in what way I can proceed. Could anyone provide me any suggestions?

Molecular prospects

We all are facing the problem of increasing climatic change and abiotic stress is increasing day by day and organic agriculture is getting challenged so how the science community will face this all.

Dear Researchers,

I am studying genome-wide identification and expression analysis of a gene family in passion fruit, which has a role in biotic and abiotic stresses, In previous reports mostly researchers used seedling to apply stresses for the analysis of the expression levels, but I was considering using the passion fruit peel or pulp as material after biotic and abiotic stresses for qPCR expressions,

1- which material will be recommended, Peel or Pulp or both?

2- For biotic stress, I considered applying different pathogenic fungi on peel and will collect the peel samples after rotten occurs (5-7 dpi), and control samples will be without fungus infection.

So that will be fine?

2- And for abiotic stresses which parameters are more suitable - low or high temperature, or should be both? according to reports, the storage temperature of passion fruit is 3-5 C, so for abiotic stress should be used <3-5 C> temperatures? For example; 0, 2, 4, 6, 8 C, and 4 C will be considered as control, so is this all right?

Your suggestions will be welcome

Regard

Rizwan

Methods that can help to find out the plant ABA content under abiotic stress.

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Why these transcription factors have fused name from two different protein For e.g AP2 play their role in flower development and ERF is ethylene response factor, But in ABA signalling in abiotic stress. We write them together as a single transcription factor protein.

One answer could be that this is different protein but shared the conserved domain or motif of AP2 and ERF.

Second answer could be that this is the superfamily name which consist both transcription factors in it. But why we mentioned it like a single protein (see in figure )in ABA signalling.

I need some clarity.

Journals submitting short communication (Plants related research)

Hello everyone, I'm doing some experiments with plants by exposing them to abiotic stress. I have estimated the DPPH inhibition percentage but don't know about what should be the trend (either decreasing or increasing)? Even I have read the literature but still confused.

I work in apricot research and we have a breeding program for abiotic stress tolerance. I collect data from the cultivars i could use in this program individually, but there are several hundred cultivars in Europe.

I would ask, if there is any database where I can find complete descriptions which is searchable? I mean if i am looking for a special trait, I can select by that criteria.

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management, biotic and abiotic stress, etc.

Which housekeeping gene is better for both biotic and abiotic stress in wheat? should we use two or more housekeeping gene? Thanks for answer.

Hydrogen gas has effects on a range of physiological events in plants. It has been shown to have effects on seed germination, plant growth, and development. It has also been found to be involved in plant stress responses and to be protective against pathological abiotic stress challenges. Similarly, it also has beneficial effects during the post-harvest storage of crops. Therefore, its use in the agricultural setting has great potential as it appears to be safe, with no toxicity or harm to the environment.

This Special Issue aims to bring together a body of papers that focus on the current state-of-play of the molecular biology and possible uses of molecular hydrogen with plants. It is hoped that this Special Issue will highlight the future work which may be undertaken in this field and help to encourage researchers to investigate this exciting field further.

For more information please follow the URL, below.

I want to establish hairy roots of my plant for abiotic stress treatment, but I want to do it without involvement of Agrobacterium. In every previously done research works, researchers have used the bacterium A. tumefaciens for infection and generation of roots. is there any other simple method which doesnt involve the bacteria?

Kindly request to experts / scientists to share opinions on spongy tissue formation causes and remedies to overcome the problem.

I have gene analysis experiment in Arabidopsis thaliana using qRT-PCR, I'm wondering which reference genes i can use knowing that i subjected the plants to abiotic stress ( abscisic acid, H2O2, HNE ( 4-hydroxynonenal)) Thanks in advance

Hello all,

I hope this question finds you well !

I'm currently working on development of indicators mixing sustainability and economy....so my question is the following:

Is the costs of resources are only affected by the market volatility of supply/demand, or it is more linked to the resource's depletion ?

If not, what could be the other indicators (global politics...) that could impact the resource price ?

Thanks,

Regards,

Nacef

Hi,

Transposable element/ Transposons are one of the main targets of genetic engineering nowadays due to their ability to alter gene expression. My query is whether TE can help plants to cope up with biotic/ abiotic stress?

In my study I have exposed the volunteers to 4 separate 45 second stressors that are a mixture of modalities (audio/visual) and task types (emotion-evoking/cognitive) with 3 minute baselines in between. A continuous ECG is trace is taken throughout the experiment, from the gross heart rate I hope to work out heart rate variability. My aim is to test the validity of heart rate variability as an objective stress assessment method for psychophysiological stress. My question is at what point on the ECG trace for each volunteer would I analyse the gross heart rate to work out Heart Rate variability for each of the four stressors i.e. pre-stressor, post-stressor and why ?

I work on some stress tolerance genes from plants. I clone those genes in pYES2 yeast expression vector and transform in to INVSc1 yeast strain to perform stress tolerance assay using different abiotic stress factos. Some months back I lost the viability of the yeast strain and now need those INVSc1 strain. Is any one know about yeast strain which I can use like INVSc1 yeast strain for stress tolerance experiment? I would really helpful to know about this.

Thanks

I urgently require fresh seeds of true and registered accessions of IR29, Pokkali, Nonabokra, N-22 and IR64 for germination and some research work on abiotic stress. I would like to ask what is the procedure to get those from central seed repositories in India like NRRI. Is it also possible to get some stocks from any lab currently working on these varieties?

Under the condition of abiotic stress，how to determine or judge the changes of photosynthetic rate are caused by stomatal factors or non-stomatal factors?

When the plant is subjected to abiotic stress, physiological, anatomical and phenotypic changes occur in the plant, as well as free radicals or the so-called reactive oxygen species (ROS) are released.

The duration and intensity of drought is increasing worldwide largely due to climate change.

Development and genetic improvement of drought tolerance crop become very important for the future due the effect of climate change.

What the best method for screening and evaluation drought stress on crop plants especially "rice"?

Thank you for the answer

Best Regards.

We are trying to increase the abiotic stress tolerance in rice by introducing certain stress tolerant genes via Agrobacterium-mediated transformation. The question I have is related to our plasmid construct. It has two selective markers, a kanamycin resistance gene (Npt1) and a glufosinate resistant gene (bar) as bacterial and plant selective markers, respectively. A CAMV35s has been used as the promoter for bar gene. Can 35s promoter function in Agrobacterium as well?

I need an updated (published in the last 5 years) book clearly and extensively explaining the concepts underlying developmental processes (embryogenesis, flower, root, shoot and leaf development) in plants and how plant endogenous signals and environmental signals are perceived and integrated into the initiation, proceeding and control of developmental processes.

Thanks in advance

Dear all scientists,

I want to do RNA-seq study on a medicinal herb which grows in wild nature. I am new in this field and I don’t how to do sampling for my experiment. I should mention each individual plant of the herb has different genotype and they are not the same genotype as a variety. I should also say that I want to use an abiotic stress to study the plant major biochemical compounds and corresponding pathways and moreover I can just afford to pay two samples to do RNA-seq. My question is: I) Should I use some genotypes for normal condition (for example the genotype A, B, C, D, E and F) and some other genotypes for treated condition (for example the genotypes  G, H, I, J, K and L). Then collect one sample from each genotypes. After forward pool samples “A” to “F”  together and also pool samples “G” to “L” together to have one pooled sample for normal condition and one pooled sample for treated condition? OR II) Should I use just ONE plant (for example, a plant from genotype “A”) for normal condition and ONE plant for treated condition (for example, a plant from genotype “G”)? OR III) Should I make some clones (vegetative propagation) from genotype “A” (for example, 6 clones from genotype “A”) and genotype “G” (for example 6 clones from genotype G), then collect samples from each plant. After forward pool 6 clones from genotype “A” together and also pool 6 clones from genotype “G” together to have one pooled sample for normal condition and one pooled sample for treated condition?   I wonder if I take solution number II or III. Since, I just use two genotypes (one genotype for normal and one genotype for stress condition), is there possibility that I miss some genes? With taking to account that it is an herb which grows in mountain and each individual plant of the herb has different genotype. Therefore the nature of each genotype is not known.

As a result, we can just choose genotypes by random. Just imagine that a sensitive genotype to the studied stress is chosen by chance. So, I will miss expression of some resistance genes in my dataset. The solution number I, is not better? Thanks in advance

In South Kalimantan, we have many rice cultivar that is potential to use as genetic resoures for breeding progam.

Some of this cultivar was cultivated in tidal swampland in South Kalimantan. Based on this fact, i have a hypotesis that this germplasm can adapted to salt stress due the wide range salinity as effect of tides.

What the best method for Screening and evaluation salt stress on rice?

Can anyone share a research paper about salt stress on rice?

Thank you for the answer

Best Regards.

Tobacco plants were subjected to 3 different nutritional treatments (A, B, C) for 3 weeks, and also were treated under two irrigation regimes during 2 weeks (Well-watered plants or Control plants, and Water deficit treated plants or drought plants). During drought plants treated with the treatments called "C" showed: (i) better water parameters, (ii) higher growth than control, (iii) higher water use efficiency and water saving, (iv) better recovery from extreme drought, etc.

Updated information:

Fresh biomass was collected in each treatment, and the following organic compounds were determined: MDA, H2O2, PROLINE, and PHENOLICS. Also, the PEROXISOME CATALASE activity was determined.

-Malonyl Dialdehyde (MDA) content, hydrogen peroxide content, and catalase activity are cellular oxidative stress biomarkers.

-Proline is a very important amino acid that which accumulation is correlated to plant stress tolerance

- Phenolics (phenolic compounds and flavonoids) are the largest group of phytochemicals that account for most of the antioxidant activity in plants.

In the attached figure there are the results of the ANOVA statistic in CTR or DROUGHT plants, and also is showing the logarithm with the base of 2 of the ratio between drought and control values to understand the decrease or increase in drought plants, in contrast, to control plants for each parameter.

What I see in this result is that there is no clear pattern related to a water deficit regarding the great results obtained in water parameters, plant biomass, water consumption/efficiency, photosynthetic activity, recovery from water stress deficit, etc.

To sum up there's complete nonsense in results in contrast to "C" treatment:

-No changes in MDA (reduction in the other nutritional treatments???????)

-Increase in CAT y H2O2???????

-a decrease in PROLINE????

-An increase in PHENOLIC compounds is logical due to the increase in H2O2, but it has no sense in plants that are more tolerant to drought.

I hope that someone might help me resolve this nonsense

I want to know the procedure how to give light treatment in case of transient expression studies. Like - to study the expression of one construct that lacks the light responsive element in its promoter.

If 2 plants belonging to the same species for, example 2 lettuce plants, are cultivated in 2 soils with different organic matter content, will their responses to abiotic stress vary?

As we know there are two types of stress biotic stress and abiotic stresses. Of which salinity is an abiotic stress and Na:K ratio is one of the factor to overcome abiotic stress.

Abiotic stress include:

- the light (excesss or not enough light)

- the air or soil humidity

- the temperature (too high or too low)

cause nutrient deficiency on plant ?

We will be testing the alleviation of salt stress in rice using Bacillus subtilis. I was wondering if a greenhouse will be necessary for this experiment.

Hi everyone,

I will be study the gene expression related to drought stress in woody plants, and I'm confused for the method to apply the stress. It Can anybody tell me what is the best way to applicate water stress on the woody seedling.

Knowing that woody seedlings are planted in soil pots.

I would like to get more information about naturally occuring osmoprotectants or compatible solutes (eg: glycine betaine, trehalose, sorbitol, fructan) in helping plants to cope with abiotic stress. Has anyone carried out related research or has good information to share?

Research is going on to increase rice yields per unit of land. Simply increase the yield will not improve the income of farmers unless the total production is properly utilized? Distress sale of paddy is very common. Just like abiotic stress, distress sale is also a stress now to rice farmers. Rice is grown under different situations such upland, irrigated, rainfed lowland i.e. shallow (water-stagnation up to 30 cm), medium depth (31-50 cm), deep water (>50 to 100 cm) etc. We need technology and proper planning to improve the income of the farmers. What they are? Any technology, which tell about only to increase yield will not increase farmers income.

What molecular mechanisms adopted by rice plants to overcome or avoid abiotic stress in relation to salt stress.

I will use gel staining to investigate the response of some plant to water stress. such plants known to accumulate terpenoid Glycosides, what enzymes should I stain?

what are the research laboratories that work on bacteria and plant interactions under environmental constraints and who accept students and researchers for training courses?

I have done some luminol based ROS assays, mainly using this protocol:

However, I only get a ROS response using FLG22. I have also tried to expose my leaf discs to high salt (150mM), sorbitol (300mM) cold (putting the 96 well plates in ice water), but none have given any ROS response.

I was wondering if anybody has any idea why this doesn't work? Or how I could make this work. I'd prefer the luminol based assays. But I will probably start using different methods if is not possible to test abiotic stresses this way.

How can efficiency of photosynthesis be increased in paddy to increased yield with increasing abiotic stress .

i am working on role of different sources of nitrogen in overcoming abiotic stress in plants. Could you provide me best sources of nitrogen in the form of nitrates used by farmers or researchers now a days?

LC-MS of rice root under abiotic stress grown under hydroponic condition.

What's the best way to measure stomatal conductance in grasses?

We are interested to carry out our research on molecular aspect of abiotic stress tolerance using mung bean as target crop.

Especially oxidative stress like UV

My work is on transcriptome analysis of plant under abiotic stress condition. I had perform RNA-seq analysis on non-treated samples (0-day), 7-days and 28-days treated samples. I'm wondering is it appropriate if I perform comparison using single-point control? or better with parallel control for each treatment time point?

Before I go for LC-MS i wish to do initial check and have a view of probable metabolites which would help me in getting better result.

As of H202 localisation studies in plant cells can we apply DAB staining for priliminary identifying of H2O2 in yeast cells under abiotic stress condition.?

Why Epigenetic Mechanisms are the key players in installing plant memory, while the genetic mechanisms comes second?

Thanks in advance.

This question is to be looked in particularly context of plants grown in induced abiotic stress

Is there any role of abiotic stress like changing temperature? or may be the deficiency of any nutrient?

There are many researchs about abiotic stress affect plant in recent years but not many project about the low light stress.

So

How low light stress affect plant physiology ?

and how we can do to improve plant resistance again low light stress ?

Good day fellow researchers,

I am planning to find some methods on how to prevent or mitigate flood stress on plants/crops physically, chemically, biologically or anything that can be suggested? Just want to familiarize some researches on flood stress. Feel free to suggest research works. Thank you!

It is well known that proline accumulates significantly as an adaptative response under stress conditions exerting many valuable functions as osmotic adjustment, protein stabilization, ROS scavenging, buffering capacity...

I am wondering if there is any decline in proline content reported under abiotic stess conditions?

Dear Colleagues,

I would like to know what is the best duration (days) to applicate salinity stress on tree seedlings aged of 60 days? i am confuse, some works said one week, 2 weeks, one month....

Thank you

please guide me. what is optimum level of SOD, MDA, Proline and Protein in rice leave? and what is their relation under salt stress conditions?

Many of us as scholars and academicians spend countless hours, sometimes consciously or unconsciously without food, exercise and so forth. Continual sitting, glued to our technological research devices leaves many of us with stress from work. Thus, there must be some efficient means of managing these stress for scholars to function properly in their research endeavors while avoiding needless ailments that can retard research progression.

we are working on improving salinity tolerance in plants  using various strategies. one of the biochemical  indicators of salinity tolerance in plants is the synthesis of the amino acid proline.

hi

im studing salt stress. i need to determine cl concentration in roots and leaves.

please help me to know a correct method.

I m interested to know adaptive features of plants in abiotic stress conditions

I am working on Drought stress tolerance on wheat, and it is found that polyamines play an important role in stress tolerance by modulating different physiological and biochemical process. i want to analyze polyamines content in plant tissues, (leaves, seed, and root). if anybody has protocol. how to measure polyamines content in plant tissues by spectrophotometer.