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ACT - Science topic

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Carpomyia vesuviana Costa is monophagous, destructive pests, and endogenous species infesting on Zizyphus spp.
I have tried many primers with gradient PCR for their molecular identification but they and not working properly.
Primers Names
X-F: 5′-ACG ATG ATG CGA TTG GTG AC-3 ′
X-R: 5′-TAT TGG TCG CGG AAC AAA CC-3 ′
COI CLepFolF 5'---ATT CAA CCA ATC ATAAAG ATA TTG G
CLepFolR 5'--TAA ACT TCT GGA TGT CCA AAA AAT CA
LCO1490 and HCO2198
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try temperature gradient and use DMSO at no more than 5% of the final volume.
as what is done for difficult PCR with low informations on genome sequence of the species you're working on.
all the best
fred
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I'm just wondering what peoples thoughts are on this. One meta-analysis I read Powers et al (2009) suggested it was more positive and outperformed CBT but not when treating depression and anxiety.
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Normally, the best and with the greatest and best scientific evidence is COGNITIVE BEHAVIORAL THERAPY (CBT o TCC)
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Hi,
Now I would like to model a simple system that a benzene on a Au(111) surface.
However, as I know the reaxFF parameter from the paper:
Jarvi, T.T., van Duin, A.C.T., Nordlund, K. and Goddard, W.A. (2011) Development of interatomic ReaxFF potentials for Au-S-C-H systems. Journal of Physical Chemistry C 115, 10315-10322
The force field was parameterized for sp3 carbon system. Does anyone have any experience to model the case for sp2?
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maybe this could work?
Susanna Monti, Vincenzo Carravetta, and Hans Ågren Simulation of Gold Functionalization with Cysteine by Reactive Molecular Dynamics J. Phys. Chem. Lett., 2016, 7 (2), pp 272-276
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Hello colleagues,
I have created an extension for Ansys workbench from a recorded script which has been recorded by Ansys ACT app builder from what I did manually in Workbench and CFD-Post. Since Ansys ACT app builder does not support CFD-Post as an stand alone product, I chose Ansys workbench as the product which I want to create the extension for it.
The extension works well and does what I did in Workbench and CFD-Post automatically but the problem is that I want to give some general inputs to it. Currently it works by the result file (.CDAT) and values which I gave in CFD-Post but I want to make the input result file (.CDAT) and some of the other parameters, general inputs for the extension.
There is a video below which shows how easy is to do this for an Ansys ACT extension which is based on a recorded script from Ansys AIM.
I want to do the same for my extension but it seems giving inputs and parameters to an Ansys ACT extension for Ansys Workbench is different and it can not be done in the same way.
I would be happy if you share your ideas with me about this problem.
Thank you in advance.
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Take a look at the Wizard examples and templates on the ACT Start Page in Workbench.
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Hi there,
I had an alarming e-mail from Clinicaltrial.gov, requesting the adding of the results in templates, just like a paper for publication, not a summary.
should I put them? and if so, should I talk to publisher first?
It was in 2016, and the completion date was before January 2017.
Part of e-mail:
FDA and NIH may take action against responsible parties if they do not submit required results information.  Failure to submit required results information is a prohibited act under the Federal Food, Drug, and Cosmetic Act, 21 U.S.C. 331(jj)(2), for which FDA could pursue civil monetary penalties under 21 U.S.C. 333(f)(3) against the ACT’s responsible party. 
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Thank you all.
I've contacted the publisher and I've got a written permission for presenting the results. They also mentioned that I should refer to the manuscript DOI when presenting the results.
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Hi All,
I am looking for recommended valid measures/questionnaires to measure a change in psychological flexibility. To be delivered pre and post intervention. Can either measure psychological flexibility as a whole, or measure the subcategories of the ACT hexaflex (or both). It is intended to be delivered to a population diagnosed with non-epileptic attacks.
All recommendations are warmly welcomed,
Thank you,
Charlotte
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This one popped up in my feed lately. Kashdan has been focusing on psych flex for a while now. https://www.toddkashdan.com/measures/
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I have a model. I made the definition of model material. After the mesh, I selected a group of elements and I would like to assign to these elements other material properties different from that of model. To be able to do this, you must create an extension with ANSYS ACT. How to create this extension please?
How to create extension of Material Assignment to element Named Selection using ansys workbench ?
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There is another way of doing this if you are going to be inserted in. I suggest that instead of having only one part in your model and dealing with defining two different types of elements to it, you can create two parts and a contact in between (like bonded contact). This way you can assign two different material to them.
You can either create the materials in workbench and then assign them to the parts or assign the material properties via command snippet in "Mechanical".
Hope this answer get you started!!
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I really need the extension for the Additive manufacturing for Ansys 19 , I can't get it from the store as I have an academic licence and can't create an account.
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Hi Yousef, did you get the ACT extension for additive manufacturing? If you did. Could you send me a copy to weihuaxian@yahoo.com?. I can't create an account as well. And I really need it. Thanks.
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What is the right logarithm to use to calculate how many people with serious mental illness need other services other than ACT or FACT?
Hello all out there _ have found calculations regarding ACT ans FACT but nothing beyond that re need and appropriateness for other services _ happy to have input from others _ Deb
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Thank you for your very quick response _ very helpful _ Deb
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Hello,
I am trying to create node coupling between two opposite faces, edges and vertices.
My geometry is cubic (or rectangular cuboid), with complex structure enclosed by smooth faces of the cube.
I understand that I need to start the mesh from the faces, couple opposite nodes, and then create volume mesh with 3D tetrahedron elements to mesh the rest of the enclosed geometry.
I tried to work through the Ansys ACT developer guide, and I do manage to write some customized files. However, I can't manage to find a way to control the mesh generation through the ACT scripting.
What will be the best way to generate this type of meshing?
Sincerely,
Gilad
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Divide the geometry into different section and on that individual section do meshing or face meshing or size meshing then you can easily customize the meshing on entire geometry.
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Hello,
I am trying to write a simple ACT script based on the user manual. 
I write both the .xml and .py files in Notepad++ and save them under the extension folder of Ansys. 
My problem is that I can't manage to see the scripted files inside ansys 'manage extension'.
My university has an academic license and I have an access to the Customer Portal. 
What should I do?
Sincerely,
Gilad 
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You get to be careful when you are writing script in Notepad++. Sometimes Notepad++ doesn't do a good job on indentation and for that reason the Python script can't be run properly. This thing happened to me before and I did not know what was really wrong until I realized that the problem is with the indentations. As far as I remember you can fix the indentation problem by doing the followings:
press Ctrl+H
in "Find what:" put four spaces
in "Replace with:" write "/t"
change the search mode to reflect "Extended"
heat "Replace All" button.
This should fix the indentation problem.
I hope you already looked that the link that Muhammad Ali put in his comment, I provide more information in there. Also, I attached the latest document that I have for ACT programming in Mechanical here.
This should get you started!!
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Just began a fellowship learning Intentive Short-Term Dynamic Therapy (ISDTP). My background is in contextual CBT's (like ACT). I'd love to know both well enough to integrate them. Does anyone have experience in this area?
Thanks,
-Dan
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@Millstein It is worth noting that those who came up with CBT were in psychoanalysis for years and the idea to shorten the frustrating process came into their mind. Cognitive-behavioral therapies and experiential dynamic therapies are based on different ways of thinking. When you come from psychodynamics it takes a while before you switch your thinking into a learning-based process even if you are very familiar with learning per se. A therapist is often eclectic nevertheless, but many think that psychodynamics and CBT are the same, they are not.
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I obtained my comparison file using blast, however when I load the comparison file on ACT it does not seem to work properly. When I select a feature (cds, inverted region) it seems to select the whole genome (everything goes yellow).. any insights?
thanks
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Hi,
Yes, the top genome is the reference. This particular region codes for some matabolism related enzymes but there´s also the presence of repetitive elements like some PE/PPE proteins, and that´s the nigthmare about mycobacterial genomes and de novo assembly when using short reads. I used ACT because I needed to detect possible re-arregements like inversions and deletions. With inversions there doesn´t seem to be many artifacts related to the de novo assembly, I think the main issue is with the possible deleted areas due to missasembly. I´m thinking about following this course of action:
For detection of Inversions: de novo assembly +ACT (this way I won´t influence the orientation of my contigs by forcing a synteny with the reference)
For detection of deletions: reference guided de novo assembly +ACT (this way I can detect the fragments that are truly missing from my genome without been influenced by missasemblies).
Again, thank you for your answers!
Carolina
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As a new professional, I will be working on an ACT Team as the only art therapy provider. Looking for any professionals in the art therapy field who are currently working or have recently worked on this type of team. Thank you in advance for any insight!
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Thank you so much Michael Uebel for your recommendation of information. Do you currently work with the ACT program?
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Dear Sir/Madam,
I need the ACT extension for ANSYS Workbench 15.0 tool [Its free software available in ANSYS Customer Portal] for selecting and exporting the X,Y,Z coordinates (3D geometry model) for particular node number. This ACT extension for ANSYS Workbench 15.0 tool can be downloaded from ANSYS customer portal by a licensed user.As I am not a licensed user I am not able to download the extension.Can anybody please download it and send it to my mail.I shall be ever grateful to him.My email id is : arbnitk@gmail.com.The link to the customer portal is - https://support.ansys.com/portal/site/AnsysCustomerPortal
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Hi
what is ACT name?
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Hi All
After creating geometry in SpaceClaim , to assign material NL hardening, how should I proceed ?
Should it definitely be created in another step ? and how should I use the same system name ?
When I use global systemname
Ansys gives me error that it is not defined , while I have created it in the step 1
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Yes. You need to use the library name. For example, if you want to call Structural Steel which is under "General Materials". then you need to change the name to "General Materials".
Create a new project and insert a static structural analysis, remove all the material that is deified (Structural steel) by default. and run the below code. This code will create
favorites1 = EngData.LoadFavoriteItems() library1 = EngData.OpenLibrary( Name="General Materials", Source="General_Materials.xml") system1 = GetSystem(Name="SYS") engineeringData1 = system1.GetContainer(ComponentName="Engineering Data") matl1 = engineeringData1.ImportMaterial( Name="Structural Steel", Source="General_Materials.xml")
You may realize that the "Source" is just a file name. This is because the file is embedded in ANSYS and there is not really a path to get access to this file. However, if you want to create your library and define your material then you need to define the path properly.
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Hello,
I am completing a data request to our State Education Agency for student level data from 2008-2017 and to our state higher education agency . The data at the public school level will contain by year, the annual testing record which includes variables such as sex, race, wealth, special education, annual test results from grade 6-8 (Reading, math, science) and high school English algebra, and biology and the ACT scores and the school last attended. I am merging this data with data from the state university system which will include application to public universities in the state, data will include variable on: application and acceptance, wealth, parent education, freshman GPA, high school GPA, school graduated from plus others.
My research questions focus on the effect of the state public schools mandating ACT testing beginning in 2011-12 and application and acceptance rates, persistence, and freshman grades (GPA) across sex, race, wealth. The data from the university system will have about 50-75 thousand students in it for each year in question.
I am considering using Regression Discontinuity design since I will have at least 4 years of data pre- and post the policy change and enough covariates to deal with any variation on samples. I also thought a Difference in Differences could work, but wanted to hear a few opinions on this before I finalized my request since I need to provide a basic description of the proposed data analysis.
Thanks for your time,
Ted
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Greg, tks
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I am trying to genotype mice from Jax (stock #: 021877) using the Sigma Kit (REDExtract-N-Amp™ Tissue PCR Kit) but I dont get bands.
I've tried the protocol suggested by Jax (https://www2.jax.org/protocolsdb/f?p=116:5:0::NO:5:P5_MASTER_PROTOCOL_ID,P5_JRS_CODE:24559,021877) and the one suggested by Sigma (https://www.sigmaaldrich.com/technical-documents/protocols/biology/redextract-n-amp.html). Changed the primers and template concentration already. My ladder works perfectly though. I got band once, but never more
These are my primers:
GCA TGT TTC CTG TTT CGT GA Wild type Forward
GCA TAT TTG GCT TTT ACT CTG G Common
TGG TGG CTG GAC CAA TGT Mutant Forward
Has anyone ever genotyped the same mice strain? Or someone who also had problems with the Sigma PCR kit?
Would appreciate any help!
Thank you.
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Hi guys, thank you for all the suggestions. I think my issue should be too much template and too little primer. Would this possibility make sense given the pic of the gel I provided? My template right now is about 300ng/uL. That is supposed to be enough, no?
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I am a firm believer in the potential benefits of ACT for a range of difficulties.
Do people have information about ACT for sleep?
Does anyone have clinical experience of using ACT-related interventions and a sense of how successful the interventions were?
Thanks!
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I've only read one study that looks at ACT for insomnia: https://www.karger.com/Article/Abstract/365173
I've used mostly CBT-I for sleep, but I imagine the mindfulness component of ACT would work quite well as would cognitive defusion for people who are kept up by thoughts... the only thing is that sleep hygiene and behavioral changes aren't inherent in the model but you could probably blend the behavioral components of CBT-I and ACT for sleep... would be an interesting interventions.
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Hi, I came across your iGuide challenge paper. I am a teacher in the ACT at a school with high number of indigenous students. As it happens I am aware of B. Bernstein. I am very influenced by Paulo Freire too. Pedagogies, school wellbeing and disadvantage is a key concern as well.
regards,
Peter
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Hi Peter, did you want to skype? Or if you're in Bris, feel free to make a time to chat. I don't have any more trips to Canberra this year, but maybe next year I might find myself in Canberra at some point. My direct email is b.exley@griffith.edu.au. Kind regards, Beryl
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I am looking for a relationship/difference between two sets of scores (state test vs. ACT). The problem is that one is on a 450-point scale and one is on a 36-point scale. I've tried the t-test, but I keep getting a straight-up "O" on it.
Can someone tell me a better test to use or a way to get these scores to be "compatible" for the t-test?
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Interested
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I conducted harmonic analysis using ANSYS workbench with memes and piezoelectric ACT extension trying to get an understanding of the behavior of the piezo material as the displacement varies harmonically. My question is about the term voltage frequency plot term mentioned in the results tab and what does it mean, and is the resulting plot represents the frequency spectrum of the output voltage that corresponds to the frequency spectrum of the input harmonic force and the resulting frequency spectrum of the displacement?
need help please to clarify things and any help regarding the piezo material simulation is also welcomed.
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As far as I understood, You are trying to create a Piezoresistive model where if you have displacement (as the results of a force or pressure) you will get different voltage. Yes. I think you got it correctly. The frequency spectrum of out put voltage corresponds to the frequency spectrum of input which can be either force or pressure. I suggest that you create an equivalent model in workbench using command snippet rather than usign ACT extension. The element you need are solid226 or solid227 (depends on what mesh you used with the keyopt of 101.
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We know the glial cells surround neurons and provide support for and insulation between them.Glial cells are the most abundant cell types in the central nervous system.But this definitions are underestimating them.
For over a century, it was believed that the glial did not play any role in neurotransmission. However 21st century neuroscience has recognized that glial cells do have some effects on certain physiological processes like breathing, and in assisting the neurons to form synaptic connections between each other. 
As I understand, glial function is self acting.A micro system to decide individually and act individually.
It recalls me to concept of  "Automata". The word automata (the plural of automaton) comes from the Greek word αὐτόματα, which means "self-acting".
The main question is if we could model the function of glial with automata?
If it's suitable even to ask this question or not?
please share your thoughts with me.
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Hi dear Mohammad Ali
by and large, cellular automata is kind of mathematical approach to solve problem like shark and fish, smart network and etc.however this approach at the basic level is determined by simple rule and it is just an assumption which the system is autonomous but we've different story in higher level. i want to refer you to spontaneous firing in the brain which a single and small fire caused a branch of firing.
last but not the least, the cellar automata decice based on the cell's neighborhood whereas the Glial act as an individual and single unit.it might possible there was a cause and effect but I've not seen in the literature yet.
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I've been trying to access safety factor result from ANSYS ACT through command lines on ACT Console. But, when i get the resultNames, no one is the safety factor result (rather than deformation, stress, etc, that appears). The image show my situation.
Please, help me. Thanks!
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Hi, Felipe.
I’m not working with ACT. I’m taking an interest in comparing the capabilities of various FE software and trying to remain abreast of their development. Your question helped me get to know something new.
Best regards.
Victor
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I am Solving a pressure vessel problem with internal pressure and the Covers bolted on to the housing. Currently all the contacts are defined as bonded (MPC). the assembly is placed on a plate which is in turn fixed, and bonded contact is defined between the vessel legs and the plate. The pretension in the bolts is 50000N given in 4 steps ( 1000N,15000, 30000, 50000). also each step is divided into minimum 50 substeps. But am getting the above error when the solver reaches the second step (15000). The UX direction in the error is along the axis of the bolt, i am unable to track the offending body as i was not able to track the pretension node through ansys ACT extension.
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There could be something wrong with the geometry of this bolt, such as two lines not meeting (where they should meet). You can try either to examine the geometry carefully or copy one of the other bolts and paste it instead of this bolt then mesh it and solve.
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We are looking at GAF and we know that HoNOS has been used for ACT before.
Looking to measure occupational functioning.
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To be effective in addictions treatment, outcomes need to be truly individualized; how deeply is the patient's addiction affectting his everyday functioning? Is he able to perform daily hygiene?
Does he need reminders? Is he able to shop and prepare meals? Does he eat 3 meals / day? measured by how much weight has been lost and is it gaining back? Is he able to maintain clean clothing, etc. Break the GAF down to parts that can be evaluated in this manner. You may want to put together a flow sheet that can be individualized. Good luck!! GBH
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I found some articles in different searches, but I have not texts or books in this field 
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For such topics, you usually can not find a specific text-book. Look at this paper and the references they have used:
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I am conducting a research focusing on the impact of maternal mental health in the childhood asthma control and I need to know which is the best test to be done in a Primary Care.
Thanks
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My pleasure. BJCP
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Some researchers said that Grain boundaries act as obstacles to slip dislocations causing dislocations to pile up on their slip planes behind the boundaries but others said that grain boundaries can become efficient sinks for dislocations. That is a contradiction for me. Can anyone help me to clarify this?
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 Dear Ahmad, Thank you very much; it was a very good explanation. Now I am more confident about grain boundaries.
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As a student of this profession, it is common to find in our work the appearance of ethical dilemmas, but I still do not understand how I could face them, besides being interested in knowing some experiences of people in that profession.
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there are many theories of ethics...find one which suit your understanding and capabilities then you can apply it in your social works....
I am a muslim so that we have our own code of ethics. however it does not mean we  reject other theories...
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Generally, data compression is the act of removing redundancy (a lack of distinction, also know as repetition or order), and as de Bruijn sequences are maximally disordered, it must be the task of data compressors to approximate de Bruijn sequences in their output.  With sufficient understanding of such translation processes, we might the decode existing de Bruijn sequences, and observe the hidden order of their message, as interpreted by decompression software.  The same goes for cryptographic processes.
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Robert: It certainly must be the case that the function of data compression is to remove redundancy.  By such removal, the yield must be an increase in disorder.  It is clear that de Bruijn sequences are maximally disordered.  Hence, it must be the case that either LZW does less than an optimal job of removing redundancy, or LZW can decompress de Bruijn sequences.  If LZW is, in fact, less than optimal, then that demonstrates an opportunity to improve compression algorithms.
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A waveform may get spikes due to lightning and other sources of surges. It is necessary to remove the spike from the waveform and compensate with an auxiliary supply.But for this the relay has to operate and reclose within few milliseconds 
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Rounak,
In the architecture of the relay, the first stage comprises of signal filtering. There are different filtering methods that are used. i.e Kalman filters, FIR filters, IRR filters etc. However, the overall challenge with these relay is that they employ Fourier Transform (FT) based methods for signal processing i.e SFT, FFT, DFT etc.  FT based methods cannot process transient signals.
One solution is to implement powerful signal processing tools such as i.e Wavelets transforms, Hilbert Huang transforms etc. together with anti aliasing filters. That way the relay may be able to effectively recognize and filter a spike. 
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I am wondering why some protein inhibitors increase the levels of target protein and still known as their inhibitors? For example MCL1 inhibitor S63845 increase the levels of MCL1 in tumor cells (Kotschy et al., Nature 538, 477–482). I understands that these inhibitors mostly act to inhibit protein-protein interactions. Like, in this case S63845 inhibits interaction of MCL1 with BAK and BAX, thereby facilitates the availability of BAK and BAX for downstream apoptotic pathway. But, It always confuses me why it is known as a inhibitor of MCL1? 
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The amount of the protein present in the cell does not equate with the amount of that protein's activity if there is an inhibitor present. The cell may respond to the lack of the target protein's activity in the presence of the inhibitor by upregulating the synthesis of that protein. Thus, more of it is made, but without effect because of the inhibitor.
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I am working on characterizing the function of a gene under stress. The T-DNA lines were obtained from SALK. The miRNA was prepared in lab and it shows very insignificant expression of my gene. Further, the 35S:over-expression lines were also generated in own lab with expression many fold to be qualified as a successful OX-lines. Currently, I am using the single T-DNA line (insertion at intron) and 3 each miRNA and Overexpression lines. I see a stress phenotype in all of them, but not in WT. Why is this weird phenotype? I understand the fact that sometimes due of presence of promoter enhancing sequences in the T-DNA, the T-DNA lines act as ox-line. But, the qRT data shows very low gene expression in my T-DNA mutant, removing the ambiguity of over-expression. Please provide your useful insight. 
Further, the miRNA can be distinguished from my OX-lines by different in root length on 1/2 MS media under normal conditions. miRNA is significantly longer than the OX-expression, WT and the T-DNA line. WT and T-DNA almost same root length. The Over-expression lines shows the shortest root lengths. 
Thanks!
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Check out incoherent feedforward loops.
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Anybody knows that when the adjuvant chemotherapy (ACT) of colon cancer was recommended for the first time in the guidelines?
The first evidence of the survival benefit of 5FU-based ACT over surgery alone in stage II-III colon cancer patients was reported from NSABP C-01 trial in 1988, which was afterward confirmed by later pooled analyses. Following these pioneering studies, 5FU deemed to be the standard control arm in newer CRTs which were consequently unable to investigate the effectiveness of the innovative surgery alone treatments (e.g. complete mesocolic excision) of colon cancer. Surprisingly, The disease-free and overall survival benefit of ACT reported in NSABP C-01 trial disappeared after ten years in an updated analysis, making a glass ceiling for the real effectiveness of ACT in clinical practice. I am working on this story and would like to know how the ACT came to the guidelines as a standard treatment of colon cancer.
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I think NSABP C-01 was the positive study showing some benefit from adjuvant chemotherapy:
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we want to to evaluated effect of acceptance commitment therapy on tolerating pain in patients with multiple sclerosis, so is there any protocol for this therapy in these patients?
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Here's what I found, from the Association for Contextual Behavioral Science website:
(see attached document)
Chronic Pain Treatment Protocol
Submitted by Kevin Vowles
An eight session protocol for chronic pain intended for use in outpatient settings. Includes preface, therapist guide, patient manual, and references.
Experimental tests to date:
Vowles, K. E., Wetherell, J. L., & Sorrell, J. T. (2009). Targeting acceptance, mindfulness, and values-based action in chronic pain: Findings of two preliminary trials of an outpatient group-based intervention. Cognitive and Behavioral Practice, 16, 49-58.
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  1. I would like to extract the S-parameter using ADS. However, i have been having some difficulties. Firstly, i cannot calculate the capacitor at the input and output of the circuit. (Both capacitor must act like a DC block to the 50 ohms termination). I have considered the Self Resonating Frequency (SRF). However, I get varying answers.
  2. What is the resolving this problem?
  3. Is the Self resonating frequency the same as the operating frequency?.
  4.  I also need to calculate the DC feed element. Do i still have to use the SRF?  Thanks for your assistance and suggestions. 
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Have a look at the attached file!
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We are studying Malaria drugs resistance, and we found that the % of early treatment failure in P. falciparum infected patients treated with ACT higher among children below 5 years.
Is this normal or explanable ?
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An echo to Dr. Kopfli
1. These under 5 yr children had relatively little acquired immunity compared with  the older individuals. 
2. Parasite density would be  higher in these young children.
Therefore, parasite clearance time might take longer in these group of children.
This means parasite clearance time at Day 3 is likely to be lower.
         By definition, ETF means inadequate decrease in parasitemia ( i.e parasitemia on day 3 ≥25% of count on day 0.)
 I feel that you have found ≥25% parasitaemina at Day 3.  
Thank you.
Thank you.
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I am curious to look at a trend in ACT data over the past 5 years or so. Is there a database that exist out there somewhere?
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I'm not sure you can directly find a database, but ACT has a research link on their website at http://www.act.org/content/act/en/research.html and may have a contact person or office there as well.
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I constructed a third-order reflective model of psychological empowerment (see model in attachment), which is composed by:
- 12 first-order latent variables: PC, ACT, CIE, OCE, PTR, PF, SI, CC, CP, OI, SN, and SB
- 3 second-order latent variables: CE (reflected in PTR, PF, and SI), RE (reflected in CC, CP, OI, SN, and SB), and BE (reflected in ACT, CIE, and OCE).
To establish the measurement model of higher-order variables, all the indicators from the lower-order variables were assigned to the higher-order variables in the form of a repeated indicators approach.
Then, CTA-PLS was used to investigate the directionality for indicators associated with the psychological empowerment construct (see CTA-PLS results in attachment)
1) Should I use significance test at p = 0.1 or 0.05 level?
2) Since I'm testing for multiple model-implied vanishing at the same time, should I assess the significance of the tetrads based on CI Low adj. and CI Up Adj. values?
3) Should I analyze both first and second-order LV results? Should I report all the results in the paper (it's a very long table...)
4) Taking into account the results, 3 first-order LV (SI, SB and SN) and 1 second-order LV (RE) are formative ([CI Low Adj., CI Up Adj.] don't include 0). Should I conclude that psychological empowerment model is better measured formatively?
Your help will be sincerely appreciated.
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Hello James,
Thank you very much for your answer. it is very pleaseful to know that you found my work interesting. I am writing a paper on this issue, I will share it with you once it is published.
Best regards,
Mariana
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Is it possible to measure the reduction of Cytochrome C by Complex III, by measuring OD at 550 nm in absence of ubiquinol? If yes, the what will act as the electron donor for Complex III?
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Thanks so much Anderson.  is there a protocol available that I can follow for using methylene blue?
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Hello everyone,
My qPCR results show that my PBMCs express pluripotency markers such as SOX2, NANOG, among others as much as my iPSCs. I found a couple of papers that discuss that a subset of PBMCs act as pluripotent stem cells.
Has anyone encountered this before?
Thanks in advance!
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When I was reading about quorum sensing mechanism, it seems to be that they are bacteriostatic. But, their are reports which are saying that QS agents can act as an antibacterial.
Can anybody suggest me the answer?
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The term antibacterial can apply to both bacteriocidal or bacteriostatic agents. So the reports that say the QS agents are antibacterial may have just not defined whether they are bacteriostatic or bacteriocidal.
Some QS inhibitors are bacteriocidal at higher concentrations, while quorum sensing inhibitory at lower concentrations. Given the nature of quorum sensing, I would also have thought that QS inhibitors (when acting as inhibitors, not bacteriocides) would be bacteriostatic. However, if the process that is regulated by QS is essential to the survival of the bacteria, then inhibition of this process would lead to mortality. However, this would mean that the bacteria would not be able to survive in sparse populations, which seems like something that would be selected out pretty quickly. Unless of course this was an inter species interaction that enhances competitiveness of one species (the QS inhibitor producer) over another (the species that needs the QS process for survival).
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The abstract says that training in use of ACT did not improve the right use. Right use dependent on general experience over time; nevertheless the conclusion ask for more training in use of ACT. I do not understand it .
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Why do you say so, when your study shows that training has little effect ? Have you examined if there is a reason that training had little effect ? "resistance to use" can well have reasons that needs to be discovered, because otherwise "training" may not address the reasons for the "resistance"
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How can i examine kinetics of ROS production?
Objective: To what extent or what amount ROS act as signalling compound and would not cause considerable oxidation and after that specific level, ROS starts oxidation.
Suggestion regarding protocols of finding kinetics of ROS
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Hi,
There are several different ways you can look at ROS production with a view to understanding to what extent the generated ROS has a signalling role and at what point ROS production results in oxidative damage. 
Probably the most common method to assess ROS production is via the use of ROS-sensitive dyes, such as DCF-DA or DHE (There are several proprietary ones also sold by several companies).  Although some of these dyes are claimed to be selective for a given ROS species when fluorescence detection is used (e.g. DHE being specific for superoxide) this is rarely the case, and at best these dyes can be used to estimate ROS levels.  However, these dyes can easily be used with plate readers/FACS/microscopy to measure ROS production over time.  This sort of approach would allow you to assess the kinetics of ROS production fairly easily.
To assess at what point oxidative damage starts occurring your best approach would be to assess several oxidative damage markers.  There are a range of different methods for assessing DNA, protein and lipid oxidative damage, and each of these techniques have associated limitations.  For example, there are commercial elisa assays for 8-oxo-dG (an oxidised DNA base) detection as well as protocols for LC-MS detection (the LC-MS methods are probably more reliable than the elisa assays).  Lipid peroxidation can be measured by TBARS, however this method does suffer from substantial artefacts.  It is also possible to assess lipid peroxidation using a dye called BODIPY C11 581/591 (this agent inserts into membranes and changes its fluorescence characteristics when oxidised). 
One final useful strategy for assessing early changes on the path to oxidative damage is the depletion of cellular antioxidants, such as glutathione.  Upregulation of enzymatic antioxidants such as SOD and CAT can also be useful markers.
Thus using a combination of the above approaches you should be able to have a good attempt at assessing ROS production and understanding at what point ROS production becomes damaging. Specific protocol information obviously depends on what equipment you have available and what your biological system is.
Hope this helps
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Hi, has anyone experience small litter size for mice. Basically I have a set of litter which are smaller than the usual size  (observed at 3 wk age) and also their tails are much shorter than normal. The male is CRE+; SEPT9:fl/ +; Act tom: mut/ mut. This is bred with female of similar genotype that is CRE+; SEPT9: fl/ +; Act tom: mut/ mut. The litters do look perfectly healthy and behave normal as other litters would at the age (3 wks). Has anyone faced small mice litter during breeding? If so, please could you share your experience as to why this happens and if they have any changes when they grow further. Thanks in advance... Susmita
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HI Sergey, many thanks for your reply. We did check the weight and the size of the litters at birth and they were usual (nothing different). We also thought that Act tom: mut/ mut for both parent mice might play a role.
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Prior to joining this site, I have found a great deal of literature on suicide prevention work with adolescents in both individual and group formats. I am familiar with ACT and DBT and I'm curious about research specifically on suicide prevention for adults. The CBT-SP model (individual and supplementary group) seems modifiable to suit adults, so I wonder if there is some reason I'm not getting that keeps us from implementing CBT-SP on a wider scale. Many troubled adults feel very "held" by structure. Regardless, the issues that must be dealt with are likely to be harrowing, and group support and experiential insight could be exponentially effective for post-crisis individuals. Aside from cohesion, mindfulness and grounding, what would you do with such a group? How can you work with the group to take hope out of the dissociative realm and make positive or rational thinking feel more real?
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Thanks for your questions, Corinna. I appreciated Tony, Melissa, and Eva's insightful comments and helpful resources.
To add to the discussion, I'd recommend an integration of psychoeducation and supportive group processes that would harness some of Yalom's therapeutic factors (e.g., universality, instillation of hope, altruism, group cohesiveness, existential factors, interpersonal learning, etc.). I've also found that "indirect compliments" from solution-focused brief therapy (SFBT) can facilitate group members' realization of their self-efficiency and self-confidence.
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After how many minutes, according to the protocol of your department, it is advisory to sample blood for ACT after heparin dose administration before the commencement of CPB?
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Once administered heparin has to mix with the blood completely and then trigger the augmentation of antithrombin and heparin cofactor II.  In my opinion the effect should be obvious if you draw a post blood sample in 2-3 minutes and perform the ACT.
Even in invitro study if you place blood in a tube containing heparin, the effect you see is immediate.  In order for the intravenously administered heparin to get completely mixed with the entire 5 litres of blood you at least require 2-4 cardiac samples and once it is mixed homogeneously with the blood it has to augment the activity of antithrombin and you may give another 1 minute so in about 2-3 minutes time there should be optimum ACT effect due to heparin.
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What is an acceptable ACT for you (PERFUSIONIST) to start the pump (cardiopulmonary bypass)?
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480 seconds
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My work aims to assess the the concept of a resurgent Keynesianism following the GFC with reference to the American Reinvestment Act and other suitable case study examples. The role of new theoretical approaches to local and regional development will also be discussed as part of this critical analysis.
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I found Clyde Prestowitz's analysis of the Post-crisis America (in his book, the Betrayal of American Prosperity) in relation to the rest of the world to be penetrating. Arguing for a return to the trajectory set in the pre-Reagan era.  Especially in terms of what type of globalization  is desirable and the areas of neglect (mostly institutional) if rectified that would put America back on a surer footing. Hailing from Asia, it gave me a peek into an alternative perspective - through the lens of an analyst that has his sights on a declining economic giant. Proving once again that decline, like prosperity, is an ever-present danger, prefiguring halts and hurdles to Asian growth if it doesn't learn from the lessons of economic history ( which may not be regional-centric and may be as relevant to all even though it concerns: America's, Europe, or even Japan for that matter). I believe we need to have a broader understanding of the underlying progression or policies and where this takes us in terms of the likely developmental trajectory. Clyde does a fantastic job of "cliometrics" while providing a running commentary on the relevant economic empirics...