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Goal is to test existing methods of gait recognition based on structured light feed...

As far as I know, confocal scanning microscope is the product of illumination PSF and detection PSF in the spatial domain, while structural illumination microscope is the product of illumination pattern of periodic structure and detection object.

If the trajectory of the point scan has a periodic structure, would the image be similar to SIM's image?

Can such images be recovered by SIM's algorithm?

From XRD data the nano particle structure is hexagonal. How can I calculate a,c and volume if my two theta value is slightly deviated from standard value?

Given the ideal case, in the optically cleared sample there must be no light scattering, hence the polarisation and coherence are preserved. Would it be possible to interfere the lighting within the optically cleared sample?

Or in case of patterned light, how far the pattern would survive inside the sample?

Can you pls recommend me some good article/ sites/ videos on how to interpret Structured Equation Model SEM regression? results/ models?

I'm using covariance-based SEM software. Do I need to normalize the data before I run the SEM model? The SEM model is fine and the data is large (744).

1. Do I need to normalize the data before I run SEM

2. Would the central limit theorem not apply and so I need not normalize the data

3. Normalizing would change the basic characteristics.Would the findings still be valid

Current devices in market has 1 color sensor, 1 IR sensor & IR illumination.

I think color sensor is only for color data.

So can I get depth inform from only 1 IR camera & 1 Structured Light illumination?

Is there a sufficient way to mount my bacterial samples (harvested from a liquid culture and fixed in glutaraldehyde) without using a filter to run them through? Because of difficulties workspace-wise I will not be able to put them on a filter and then prepare them for SEM. Would it be feasible (after further washing, sec.fixing and dehydrating) to put some of the suspension onto a round cover slip for mounting on a stub? I'm very grateful for some insights or ideas! Thanks!

I am interested in studying biofilm formation as a function of multiple variables, and would like to go beyond spectrophotometric/colorimetric/microplate readings and to analyze biofilm structures microscopically as well. Unfortunately, fluorescent microscopy and confocal laser scanning microscopy, tools that are routinely used by well-funded research groups for this purpose, are currently not at my disposal.

To this end, I am considering to utilize traditional staining (e.g., crystal violet) of these structures followed by simple light microscopy. May I ask for recommendations on objectively/quantitatively analyzing the images that can be generated this way (perhaps through a specific software)? Thank you!