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Gullnut extract (which contains primary tannic acid ) turns green upon raising pH using sodium carbonate and leaving it for 2-3 mins
why does this happens ?
the decomposition components of tannic acids do have such colors ?
We are conducting an active measurement experiment of Beta-hexosaminidase (B-hexo) through RBL-2H3 cells. The courses are as follows: 1. Seed RBL-2H3 cells to 96 well plates at 500 ng/ml with a final concentration of anti-DNP-IgE of 8 x 10^4/well and incubate for a day. 2. Remove the top liquid and wash it twice with DPBS. 3. 2 hr pre incubation with Siraganian buffer 4. Remove the supermatamt and culture the DNP-BSA dissolved in the Siraganian buffer for 30 minutes. (Final concentration: 150ng/ml) 5. Transfer 60ul of the supernatant to a new 96 well plate and react 3 hr at 37°C with a 10mM
P-NAG (in 0.1M Citrate buffer) of 40ul. 6. Stop the reaction with 0.05M sodium carbonate 100ul and measure absorbance at 405nm.
However, no B-hexo was created in positive control. What's wrong with my process?
Please if you have a source about the phase equlibria fundamentals would be greatly appreciated? and how to construct them/
Does Sodium Carbonate (Na2CO3) decompose completely at >900ºC (1000ºC or 1100ºC) to Sodium Oxide? Or it´s a partial decomposition?
To produce >95% pure Sodium Oxide what salt is better to use: Sodium Carbonate or Sodium Nitrite, or other?
Need complete answer.
Thanks for all answers.