Hello everyone,
In the literature, it's very clear that when preparing otoliths for trace element analysis by LA-ICP-MS, people who section (cut) their otoliths subsequently polish them.
I am trying to determine if the polishing step is actually necessary, given logistical constraints in a current project that I'm participating in. Our current lab setup allows us to very accurately section otoliths and expose their core.
Cons of polishing are: (1) it's very time & labour intensive, (2) it potentially introduces surface contamination, either from the polishing surface or from other otoliths.
Pros of polishing are: (1) it evens out the otolith surface, which may be rough from the cutting process, (2) it increases the clarity of otolith microstructures (e.g. increments), (3) it makes sure the core is exposed (which we can already do using our cutting method).
What are your thoughts on this?