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Did someone faced problem with clarity measurements with turbinometer?

We encountered a problem with the analysis of clarity of solutions. As per Ph.Eur. reference suspension I should have 3 NTU, reference II 6 NTU, however we measured around 4.7 and 9.6 respectively.

We prepared several solution, all the same.

We bought Primary opalescent suspension with Assay on CoA of 4094 NTU, obtained standard of opalescence is 79.4 NTU.

We use a 4 points calibration.

I cannot understand what more should be done?

Any suggestions? Thank you in advance!

Hello everyone,

The computer with the Clarity software driving a Biotek microplate luminometer crashed and we can not find the software disk to install it on another computer. Received no answer from Agilent, now owner of Biotek. Any ideas? Thank you

I'd like to try the CLARITY method on some of my knock-in mice with fluorescent reporter molecules attached to their post synaptic proteins. It is impractical for me to set up the entire method when I don't know if it will kill the fluorescence, so it would be excellent if I could come and borrow someone's set up for a short period to test the method on my samples.

Any leads to how this data can be transformed to distances? Transformation techniques? software? Currently reading on Mahalanobis distances and  Gowers (dis)similarity coefficient but there is still too much ground to cover. All productive detours are welcome. Thank you

Can't seem to find whether or not CLARITY is able to be performed on intact brains without quenching or washing away injected tracers (non-viral or genetic expression of fluorescence) such as dextrans or beads.

Does anyone know if any clearing method is compatible with tracer injections?

we are using the CLARITY technique to clear Rat brains and we would like to label astrocytes and neurons in the cerebral cortex and to be able to count them. We tried to stain it using NeuN and GFAP, but it didn’t stain. Do you have any recommendations/thoughts on why it did not work, or any specific steps we should follow? we followed steps on 

Some studies have found that fasting can help support recovery from brain injuries and strokes and lower the risk for developing neurodegenerative diseases like Alzheimer’s, dementia and Parkinson’s disease.

I just posted this in the CLARITY resource center forum, but posting it here too. We found this hydrogel perfused/embedded right hemisphere of a rat cortex to be nearly completely clear after sitting in clearing solution in 37 degree incubator for ~5 weeks completely unattended. Next to zero tissue damage and it looks by far better than any brain that we have run through our ETC chamber. Has anyone else seen this?

I want to image skin tissue in depth by confocal microscopy and my target is dermal-epidermal junction. So, thinking that optical clearing/clarity might help in this regard.

Thanks.

I am conduction a research on physico chemical characteristics of starch.  I have got this protocol online.  

Paste clarity was determined by the method of Singhal and

Kulkarni (1990) by measuring the percentage light transmitted

by different concentrations of starch (0.5–4.0%) at 660 nm on a

UV/visible spectrophotometer. Distilled water was used in the reference

cell.

Do we have to let the paste cool down ?